FvH4_3g04350

Endonuclease 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007730
Physical Location & Seq
Forward (+)
2486427 .. 2490004
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04350.t1

Sequence Viewer

Length: 891 bp
ATGGGTTTGTTTGGGGTGTGGTGGAGTGGAAGTGTGGTAGGGTTTCTTGTGTTGGTGCCAGGAATTCTGGGTTGGGGCAAAGAAGGCCACTATGCAGTTTGCAAGATAGCAGAGGGATTTCTTAGTGAAGATGCTTTAGCTGCTGTGAAAGAATTACTACCAGAATCTGCTGGAGGTGATCTTGCTGCAGTTTGCTCCTGGCCTGATGAGATTCGCTTTCATTATCGGTGGAGTAGTGCTTTGCACTATGTTGACACACCGGATTTTCTGTGTAACTACAATGACAGCAGAGACTGCCATGACTCTGCTGGACATAAAGGTAGATGTGTAACTGCAGCAATTAACAACTATACGATGCAGCTTTTGACTTATCAGGATTCCATCTCAAAATCAAAATACAACTTAACAGAGGCACTTCTTTTCTTGTCTCACTTTATTGGGGATATCCATCAGCCCCTACATGTTGGTTTCACTGGAGACCTAGGGGGGAACACAATAATAGTCCGTTGGTATCACAGGAAGACAAATCTTCACCATGTATGGGATAATATGATCATTGAATCTGCTGTAAAGACAATATATGGGAACAATCTTGAACTCATGATAGAGACTATTCAGAGGAATATTACAGATGCTTGGTTCAGTGATTTGTCATCATGGCAGTTTTGTGCAAATAATTGGAGTACTGTATGTCCAAATGAGTATGCTTCTGAAAGCATTCGTTTGGCATGCAAATTTGCTTACAGGAATGCCACTCCCGGAAGCACTCTGAATGATGATTACTTTGTTTCTCGGCTGCCTATAGTGGAGAAGCGGCTAGCTCAAAGTGGGGTCCGCTTGGCTGCTACCCTCAATCATATCTTCTCTGAACCAAAAATTGCTGGAGCATGA

Protein Analysis

297

Amino Acids

33.12

Weight (kDa)

5.92

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 25 - 288 1.5e-83 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AciI CCGC 2 cut(s) 814, 835
AcsI RAATTY 2 cut(s) 63, 734
AfaI GTAC 1 cut(s) 685
AfiI CCNNNNNNNGG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 2 cut(s) 560, 596
AjnI CCWGG 2 cut(s) 58, 197
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 3 cut(s) 140, 361, 821
AluI AGCT 3 cut(s) 140, 361, 821
Alw26I GTCTC 4 cut(s) 285, 432, 471, 602
AlwNI CAGNNNCTG 2 cut(s) 167, 294
AoxI GGCC 2 cut(s) 85, 200
ApeKI GCWGC 6 cut(s) 140, 185, 335, 358, 796, 842
ApoI RAATTY 2 cut(s) 63, 734
Asp700I GAANNNNTTC 1 cut(s) 717
AspA2I CCTAGG 1 cut(s) 481
AspS9I GGNCC 1 cut(s) 832
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 2 cut(s) 188, 524
AsuNHI GCTAGC 1 cut(s) 817
AvaII GGWCC 1 cut(s) 832
AvrII CCTAGG 1 cut(s) 481
BanI GGYRCC 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 527
BbvI GCAGC 6 cut(s) 127, 172, 347, 370, 783, 829
BccI CCATC 2 cut(s) 389, 456
BciT130I CCWGG 2 cut(s) 60, 199
BclI TGATCA 1 cut(s) 552
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 4 cut(s) 285, 432, 471, 602
BfaI CTAG 2 cut(s) 482, 818
BfmI CTRYAG 3 cut(s) 186, 333, 801
BisI GCNGC 7 cut(s) 141, 186, 336, 359, 797, 815, 843
BlnI CCTAGG 1 cut(s) 481
BlsI GCNGC 7 cut(s) 142, 187, 337, 360, 798, 816, 844
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 3 cut(s) 60, 199, 759
Bme18I GGWCC 1 cut(s) 832
BmgT120I GGNCC 1 cut(s) 832
BmiI GGNNCC 2 cut(s) 57, 833
BmrFI CCNGG 3 cut(s) 60, 199, 759
BmsI GCATC 3 cut(s) 121, 345, 622
BmtI GCTAGC 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 527
BpmI CTGGAG 2 cut(s) 192, 495
BpuMI CCSGG 1 cut(s) 759
BsaBI GATNNNNATC 1 cut(s) 447
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 478
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 2 cut(s) 60, 199
BseDI CCNNGG 1 cut(s) 481
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 541
BseNI ACTGG 1 cut(s) 478
BseXI GCAGC 6 cut(s) 127, 172, 347, 370, 783, 829
BshFI GGCC 2 cut(s) 87, 202
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 2 cut(s) 260, 759
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 4 cut(s) 285, 432, 471, 602
BsmI GAATGC 2 cut(s) 717, 754
BsnI GGCC 2 cut(s) 87, 202
Bso31I GGTCTC 1 cut(s) 471
Bsp143I GATC 2 cut(s) 178, 552
BspACI CCGC 2 cut(s) 814, 835
BspANI GGCC 2 cut(s) 87, 202
BspHI TCATGA 1 cut(s) 600
BspLI GGNNCC 2 cut(s) 57, 833
BspMAI CTGCAG 2 cut(s) 190, 337
BspOI GCTAGC 1 cut(s) 821
BspT107I GGYRCC 1 cut(s) 55
BspTNI GGTCTC 1 cut(s) 471
BsrI ACTGG 1 cut(s) 478
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 2 cut(s) 178, 552
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 2 cut(s) 60, 199
Bst4CI ACNGT 1 cut(s) 688
BstAPI GCANNNNNTGC 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 730, 819
BstDEI CTNAG 1 cut(s) 122
BstKTI GATC 2 cut(s) 181, 555
BstMAI GTCTC 4 cut(s) 285, 432, 471, 602
BstMBI GATC 2 cut(s) 178, 552
BstMWI GCNNNNNNNGC 3 cut(s) 84, 140, 294
BstNI CCWGG 2 cut(s) 60, 199
BstNSI RCATGY 2 cut(s) 464, 732
BstSCI CCNGG 3 cut(s) 58, 197, 757
BstSFI CTRYAG 3 cut(s) 186, 333, 801
BstV1I GCAGC 6 cut(s) 127, 172, 347, 370, 783, 829
BstV2I GAAGAC 1 cut(s) 527
BsuRI GGCC 2 cut(s) 87, 202
BtsIMutI CAGTG 2 cut(s) 471, 649
Cac8I GCNNGC 2 cut(s) 730, 819
CaiI CAGNNNCTG 2 cut(s) 167, 294
CciI TCATGA 1 cut(s) 600
Cfr13I GGNCC 1 cut(s) 832
Csp6I GTAC 1 cut(s) 684
CviAII CATG 7 cut(s) 299, 461, 536, 601, 657, 729, 888
CviJI RGCY 9 cut(s) 87, 140, 202, 361, 454, 796, 817, 821, 842
CviKI_1 RGCY 9 cut(s) 87, 140, 202, 361, 454, 796, 817, 821, 842
CviQI GTAC 1 cut(s) 684
DdeI CTNAG 1 cut(s) 122
DpnI GATC 2 cut(s) 180, 554
DpnII GATC 2 cut(s) 178, 552
Eco130I CCWWGG 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 471
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 832
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 58, 197
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 7 cut(s) 302, 464, 539, 604, 660, 732, 891
FatI CATG 7 cut(s) 298, 460, 535, 600, 656, 728, 887
FbaI TGATCA 1 cut(s) 552
Fnu4HI GCNGC 7 cut(s) 141, 186, 336, 359, 797, 815, 843
Fsp4HI GCNGC 7 cut(s) 141, 186, 336, 359, 797, 815, 843
FspBI CTAG 2 cut(s) 482, 818
GluI GCNGC 7 cut(s) 141, 186, 336, 359, 797, 815, 843
GsuI CTGGAG 2 cut(s) 192, 495
HaeIII GGCC 2 cut(s) 87, 202
HapII CCGG 2 cut(s) 260, 759
Hin1II CATG 7 cut(s) 302, 464, 539, 604, 660, 732, 891
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 5 cut(s) 164, 211, 302, 377, 560
HpaII CCGG 2 cut(s) 260, 759
HphI GGTGA 2 cut(s) 188, 524
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 4 cut(s) 618, 712, 771, 868
Hpy188III TCNNGA 3 cut(s) 374, 593, 601
Hpy8I GTNNAC 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4V TGCA 8 cut(s) 95, 102, 188, 244, 335, 358, 671, 732
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 140, 294
HpyF3I CTNAG 1 cut(s) 122
Hsp92II CATG 7 cut(s) 302, 464, 539, 604, 660, 732, 891
Ksp22I TGATCA 1 cut(s) 552
Kzo9I GATC 2 cut(s) 178, 552
LmnI GCTCC 2 cut(s) 200, 884
Lsp1109I GCAGC 6 cut(s) 127, 172, 347, 370, 783, 829
LweI GCATC 3 cut(s) 121, 345, 622
MaeI CTAG 2 cut(s) 482, 818
MaeIII GTNAC 2 cut(s) 272, 328
MalI GATC 2 cut(s) 180, 554
MboI GATC 2 cut(s) 178, 552
MboII GAAGA 4 cut(s) 140, 521, 532, 853
MluCI AATT 6 cut(s) 63, 152, 339, 676, 734, 876
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 5 cut(s) 106, 167, 403, 612, 860
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 2 cut(s) 342, 404
MspI CCGG 2 cut(s) 260, 759
MspR9I CCNGG 3 cut(s) 60, 199, 759
Mva1269I GAATGC 2 cut(s) 717, 754
MvaI CCWGG 2 cut(s) 60, 199
MwoI GCNNNNNNNGC 3 cut(s) 84, 140, 294
NciI CCSGG 1 cut(s) 759
NdeII GATC 2 cut(s) 178, 552
NheI GCTAGC 1 cut(s) 817
NlaIII CATG 7 cut(s) 302, 464, 539, 604, 660, 732, 891
NlaIV GGNNCC 2 cut(s) 57, 833
NmeAIII GCCGAG 1 cut(s) 772
NspI RCATGY 2 cut(s) 464, 732
PaeI GCATGC 1 cut(s) 732
PagI TCATGA 1 cut(s) 600
PciI ACATGT 1 cut(s) 460
PctI GAATGC 2 cut(s) 717, 754
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 4 cut(s) 164, 211, 377, 560
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 7 cut(s) 142, 187, 337, 360, 798, 816, 844
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PscI ACATGT 1 cut(s) 460
Psp6I CCWGG 2 cut(s) 58, 197
PspGI CCWGG 2 cut(s) 58, 197
PspN4I GGNNCC 2 cut(s) 57, 833
PspPI GGNCC 1 cut(s) 832
PstI CTGCAG 2 cut(s) 190, 337
PstNI CAGNNNCTG 2 cut(s) 167, 294
RsaI GTAC 1 cut(s) 685
RsaNI GTAC 1 cut(s) 684
SaqAI TTAA 2 cut(s) 342, 404
SatI GCNGC 7 cut(s) 141, 186, 336, 359, 797, 815, 843
Sau3AI GATC 2 cut(s) 178, 552
Sau96I GGNCC 1 cut(s) 832
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 1 cut(s) 296
ScrFI CCNGG 3 cut(s) 60, 199, 759
SetI ASST 6 cut(s) 142, 178, 322, 363, 483, 823
SfaNI GCATC 3 cut(s) 121, 345, 622
SfcI CTRYAG 3 cut(s) 186, 333, 801
SinI GGWCC 1 cut(s) 832
SphI GCATGC 1 cut(s) 732
Sse9I AATT 6 cut(s) 63, 152, 339, 676, 734, 876
SsiI CCGC 2 cut(s) 814, 835
SspI AATATT 1 cut(s) 625
SspMI CTAG 2 cut(s) 482, 818
StyD4I CCNGG 3 cut(s) 58, 197, 757
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 1 cut(s) 688
TasI AATT 6 cut(s) 63, 152, 339, 676, 734, 876
TatI WGTACW 1 cut(s) 683
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 4 cut(s) 164, 211, 377, 560
Tru1I TTAA 2 cut(s) 342, 404
Tru9I TTAA 2 cut(s) 342, 404
TscAI CASTG 2 cut(s) 478, 649
TseI GCWGC 6 cut(s) 140, 185, 335, 358, 796, 842
TspDTI ATGAA 1 cut(s) 209
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 2 cut(s) 478, 649
VpaK11BI GGWCC 1 cut(s) 832
XapI RAATTY 2 cut(s) 63, 734
XceI RCATGY 2 cut(s) 464, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XmaJI CCTAGG 1 cut(s) 481
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 2 cut(s) 482, 818
ZrmI AGTACT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.