AT4G21590

endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007730
Physical Location & Seq
Forward (+)
11480321 .. 11482765
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21590.1

Sequence Viewer

Length: 885 bp
ATGGGTTGGTCGTTGAGAATGTGGATTGTGAGTATACTCGTGCTTACACAGCTCGTTAATGGAGCTCTCTGTTGGGGAGACGCTGGTCATTACGCTGTCTGCAAAATAGCTCAGAGTTATTTTGAGGAAGATACTGTAGTTGCTGTGAAGAAACTTCTGCCTGAATCTGCAAATGGTGAGCTAGCAGCTGTTTGCTCATGGCCTGATGAAATCAAAAAGCTCCCGCAATGGCGATGGACTAGTGCTTTGCATTTTGCTGATACGCCTGATTACAAATGCAACTATGAGTACTCTCGGGACTGTCCTAAAGACTGGTGTGTGACAGGGGCAATCTTCAATTACACTAACCAACTAATGTCTACTTCTGAAAACTCACAGAGTATAGTCCACTATAACTTAACAGAGGCTCTCATGTTCTTATCACATTATATGGGAGATATCCATCAGCCTTTACATGAAGGTTTTATTGGAGATCTAGGTGGTAACAAGATAAAAGTTCACTGGTACAATCAGGAAACTAATTTGCACCGTGTGTGGGATGATATGATAATTGAGTCTGCTCTAGAAACATACTACAATTCAAGCCTTCCACGCATGATTCATGAACTTCAAGCCAAACTCAAGAACGGCTGGTCAAACGATGTACCTTCATGGGAGTCATGTCAACTTAACCAAACGGCCTGCCCAAATCCGTATGCTTCTGAAAGCATTGATCTTGCCTGCAAGTATGCTTACAGGAACGCCACCGCAGGGACTACTTTAGGAGATTACTATTTCGTCTCTCGGTTACCGGTGGTAGAGAAGAGACTTGCACAAGGCGGAATTCGCTTGGCAGGGACTCTTAACCGAATATTTTCTGCAAAACGGAAGCTTGCTAGAGCATGA

Protein Analysis

294

Amino Acids

33.42

Weight (kDa)

6.3

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 25 - 285 3.5e-86 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 34, 359
AciI CCGC 3 cut(s) 224, 747, 819
AcsI RAATTY 1 cut(s) 822
AdeI CACNNNGTG 1 cut(s) 532
AfaI GTAC 3 cut(s) 290, 506, 645
AfiI CCNNNNNNNGG 2 cut(s) 535, 750
AgeI ACCGGT 1 cut(s) 790
AgsI TTSAA 3 cut(s) 337, 582, 611
AhlI ACTAGT 1 cut(s) 239
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AluBI AGCT 7 cut(s) 52, 65, 110, 181, 188, 220, 871
AluI AGCT 7 cut(s) 52, 65, 110, 181, 188, 220, 871
Alw21I GWGCWC 1 cut(s) 67
Alw26I GTCTC 3 cut(s) 72, 784, 799
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 2 cut(s) 200, 678
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 1 cut(s) 822
ArsI GACNNNNNNTTYG 2 cut(s) 229, 261
AsiGI ACCGGT 1 cut(s) 790
Asp700I GAANNNNTTC 1 cut(s) 853
AsuHPI GGTGA 1 cut(s) 188
AsuNHI GCTAGC 1 cut(s) 181
AvaI CYCGRG 1 cut(s) 294
BaeI ACNNNNGTAYC 2 cut(s) 123, 156
BanII GRGCYC 1 cut(s) 67
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 67
BbvI GCAGC 1 cut(s) 197
BccI CCATC 2 cut(s) 228, 450
BceAI ACGGC 2 cut(s) 643, 693
BcoDI GTCTC 3 cut(s) 72, 784, 799
BcuI ACTAGT 1 cut(s) 239
BfaI CTAG 5 cut(s) 182, 240, 476, 563, 876
BfmI CTRYAG 1 cut(s) 135
BglII AGATCT 1 cut(s) 472
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
BmcAI AGTACT 1 cut(s) 290
BmeT110I CYCGRG 1 cut(s) 294
BmtI GCTAGC 1 cut(s) 185
BoxI GACNNNNGTC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 605
BsaBI GATNNNNATC 1 cut(s) 441
BsaWI WCCGGW 1 cut(s) 790
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 750
Bse118I RCCGGY 1 cut(s) 790
Bse1I ACTGG 2 cut(s) 317, 506
Bse3DI GCAATG 1 cut(s) 233
Bse8I GATNNNNATC 1 cut(s) 441
BseGI GGATG 1 cut(s) 544
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 2 cut(s) 535, 750
BseMI GCAATG 1 cut(s) 233
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 2 cut(s) 317, 506
BseXI GCAGC 1 cut(s) 197
BshFI GGCC 2 cut(s) 202, 680
BshTI ACCGGT 1 cut(s) 790
BsiHKAI GWGCWC 1 cut(s) 67
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 3 cut(s) 311, 766, 850
BslI CCNNNNNNNGG 2 cut(s) 535, 750
BsmAI GTCTC 3 cut(s) 72, 784, 799
BsmBI CGTCTC 2 cut(s) 72, 784
BsmFI GGGAC 3 cut(s) 311, 766, 850
BsnI GGCC 2 cut(s) 202, 680
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 67
Bsp143I GATC 2 cut(s) 472, 712
BspACI CCGC 3 cut(s) 224, 747, 819
BspANI GGCC 2 cut(s) 202, 680
BspCNI CTCAG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 601
BspOI GCTAGC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 790
BsrI ACTGG 2 cut(s) 317, 506
BssAI RCCGGY 1 cut(s) 790
BssMI GATC 2 cut(s) 472, 712
BssNAI GTATAC 1 cut(s) 35
BssSI CACGAG 1 cut(s) 38
Bst1107I GTATAC 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 38
Bst4CI ACNGT 3 cut(s) 136, 302, 530
Bst6I CTCTTC 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 183, 682, 721, 873
BstDEI CTNAG 1 cut(s) 111
BstEII GGTNACC 1 cut(s) 786
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 2 cut(s) 475, 715
BstMAI GTCTC 3 cut(s) 72, 784, 799
BstMBI GATC 2 cut(s) 472, 712
BstMWI GCNNNNNNNGC 3 cut(s) 49, 591, 825
BstPAI GACNNNNGTC 1 cut(s) 84
BstPI GGTNACC 1 cut(s) 786
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 472
BstYI RGATCY 1 cut(s) 472
BstZ17I GTATAC 1 cut(s) 35
BsuRI GGCC 2 cut(s) 202, 680
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 4 cut(s) 183, 682, 721, 873
CciI TCATGA 1 cut(s) 601
Cfr10I RCCGGY 1 cut(s) 790
CseI GACGC 1 cut(s) 89
Csp6I GTAC 3 cut(s) 289, 505, 644
CspAI ACCGGT 1 cut(s) 790
CviAII CATG 8 cut(s) 198, 412, 455, 595, 602, 651, 660, 882
CviQI GTAC 3 cut(s) 289, 505, 644
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 474, 714
DpnII GATC 2 cut(s) 472, 712
DraIII CACNNNGTG 1 cut(s) 532
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
EciI GGCGGA 1 cut(s) 834
Ecl136II GAGCTC 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 67
Eco32I GATATC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 294
Eco91I GGTNACC 1 cut(s) 786
EcoICRI GAGCTC 1 cut(s) 65
EcoO65I GGTNACC 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 822
EcoRV GATATC 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 67
Esp3I CGTCTC 2 cut(s) 72, 784
FaeI CATG 8 cut(s) 201, 415, 458, 598, 605, 654, 663, 885
FaqI GGGAC 3 cut(s) 311, 766, 850
FatI CATG 8 cut(s) 197, 411, 454, 594, 601, 650, 659, 881
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 2 cut(s) 34, 359
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 551
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 5 cut(s) 182, 240, 476, 563, 876
GluI GCNGC 1 cut(s) 186
HaeIII GGCC 2 cut(s) 202, 680
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 89
Hin1II CATG 8 cut(s) 201, 415, 458, 598, 605, 654, 663, 885
HincII GTYRAC 1 cut(s) 665
HindII GTYRAC 1 cut(s) 665
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 5 cut(s) 164, 554, 598, 656, 838
HpaII CCGG 1 cut(s) 791
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 5 cut(s) 35, 360, 388, 499, 665
Hpy188I TCNGA 3 cut(s) 114, 367, 703
Hpy188III TCNNGA 5 cut(s) 296, 512, 563, 602, 622
Hpy8I GTNNAC 5 cut(s) 35, 360, 388, 499, 665
HpyAV CCTTC 3 cut(s) 452, 596, 657
HpyCH4III ACNGT 3 cut(s) 136, 302, 530
HpyCH4V TGCA 8 cut(s) 102, 170, 250, 279, 526, 723, 812, 860
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 591, 825
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 8 cut(s) 201, 415, 458, 598, 605, 654, 663, 885
Kzo9I GATC 2 cut(s) 472, 712
LmnI GCTCC 2 cut(s) 62, 225
Lsp1109I GCAGC 1 cut(s) 197
MaeI CTAG 5 cut(s) 182, 240, 476, 563, 876
MaeIII GTNAC 3 cut(s) 319, 482, 786
MalI GATC 2 cut(s) 474, 714
MboI GATC 2 cut(s) 472, 712
MboII GAAGA 4 cut(s) 140, 160, 325, 814
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 67
MluCI AATT 5 cut(s) 337, 520, 549, 577, 822
MlyI GAGTC 3 cut(s) 563, 665, 832
MnlI CCTC 2 cut(s) 118, 397
MroXI GAANNNNTTC 1 cut(s) 853
MseI TTAA 4 cut(s) 57, 398, 669, 843
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 791
MwoI GCNNNNNNNGC 3 cut(s) 49, 591, 825
NdeII GATC 2 cut(s) 472, 712
NheI GCTAGC 1 cut(s) 181
NlaIII CATG 8 cut(s) 201, 415, 458, 598, 605, 654, 663, 885
NmuCI GTSAC 1 cut(s) 319
PagI TCATGA 1 cut(s) 601
PdmI GAANNNNTTC 1 cut(s) 853
PfeI GAWTC 2 cut(s) 164, 598
PinAI ACCGGT 1 cut(s) 790
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 3 cut(s) 562, 664, 832
PpsI GAGTC 3 cut(s) 562, 664, 832
PshAI GACNNNNGTC 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 67
PspEI GGTNACC 1 cut(s) 786
PsuI RGATCY 1 cut(s) 472
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 3 cut(s) 290, 506, 645
RsaNI GTAC 3 cut(s) 289, 505, 644
SacI GAGCTC 1 cut(s) 67
SaqAI TTAA 4 cut(s) 57, 398, 669, 843
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 472, 712
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 3 cut(s) 563, 665, 832
SduI GDGCHC 1 cut(s) 67
SfcI CTRYAG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
SpeI ACTAGT 1 cut(s) 239
Sse9I AATT 5 cut(s) 337, 520, 549, 577, 822
SsiI CCGC 3 cut(s) 224, 747, 819
SspI AATATT 1 cut(s) 852
SspMI CTAG 5 cut(s) 182, 240, 476, 563, 876
SstI GAGCTC 1 cut(s) 67
TaaI ACNGT 3 cut(s) 136, 302, 530
TasI AATT 5 cut(s) 337, 520, 549, 577, 822
TatI WGTACW 1 cut(s) 288
TfiI GAWTC 2 cut(s) 164, 598
Tru1I TTAA 4 cut(s) 57, 398, 669, 843
Tru9I TTAA 4 cut(s) 57, 398, 669, 843
TscAI CASTG 1 cut(s) 506
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 5 cut(s) 222, 471, 590, 618, 639
TspGWI ACGGA 2 cut(s) 681, 880
TspRI CASTG 1 cut(s) 506
XapI RAATTY 1 cut(s) 822
XbaI TCTAGA 1 cut(s) 562
XmiI GTMKAC 2 cut(s) 34, 359
XmnI GAANNNNTTC 1 cut(s) 853
XspI CTAG 5 cut(s) 182, 240, 476, 563, 876
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.