MD05G1323700.v1.1

Endonuclease 4-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007730
Physical Location & Seq
Reverse (-)
45084052 .. 45087091
3040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1323700.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGGTGGGACTGGGTTTTGGTGGACTTGGAGGGTGCTTGGGTCTATGCTATTGGTGCCAGGAATTCTGGGTTGGGGCAAAGAAGGCCACTACACAATTTGCAAGATTGCAGAGGGATTTCTGAGTGAAGAGGCTCTCGCTGCGGTGAAAGAGTTGCTACCGGCATCTGCCAAAGGTGATCTTGCAGAAGTTTGCTCCTGGCCTGATGAGATTCGTCATAGCTACCATTGGCGATGGAGTGGTCCTTTGCACTATGTTGACACACCGGATTTTAAGTGTAACTACAAATACTGCAGAGACTGCCATGACTCTGCTGGACATAAAGATAGGTGTGTCACTGCAGCGATTTACAACTATTCGATGCAACTCTCTTCGGGTTACCATGAGTCCATCTCAGAATCAGGATACAACTTAACAGAGGCACTTATGTTCTTGTCTCATTTTATTGGGGATGTGCATCAGCCCCTACATGTTGGCTTCACTGGAGATGAAGGCGGGAACACAATAATAGTCCGATGGTATCGCAGGAAGACGAATCTCCACCATGTCTGGGATAATATGATCATTGAATCTGCTGTAAAGACATACTACAGTAAGGATCTTGCAATCATGATAAAGGCCATTGAAAGGAATATTACGGATGGTTGGTTCAATGTTATACCGTCATGGAAGATTTGTGCAAATAATCAGACTGTATGTCCGAACGAGCATGCCTCTGAAAGCATTAGTTTGGCATGCAAATATGCTTACAGAAATGCCACACCTGGAAGCACACTGACAGATGACTACTTCCTCTCTCGGCTACCTGTTGTGGAGAAGAGGCTAGCTCAAAGTGGGGTCCGCCTGGCTGCTACCCTCAATCGTATTTTCTCTTCCCAAGCAAAACAAATTGCTGATGCATGA

Protein Analysis

301

Amino Acids

33.85

Weight (kDa)

6.66

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1-P1_nuclease PF02265 25 - 290 1.5e-84 S1/P1 Nuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 55
AciI CCGC 3 cut(s) 143, 495, 841
AclWI GGATC 1 cut(s) 606
AcsI RAATTY 1 cut(s) 63
AfiI CCNNNNNNNGG 2 cut(s) 550, 627
AflIII ACRYGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 569, 626, 652
AhdI GACNNNNNGTC 1 cut(s) 696
AjnI CCWGG 4 cut(s) 58, 197, 763, 843
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AloI GAACNNNNNNTCC 2 cut(s) 632, 664
AluBI AGCT 2 cut(s) 222, 827
AluI AGCT 2 cut(s) 222, 827
Alw26I GTCTC 2 cut(s) 291, 441
AlwI GGATC 1 cut(s) 606
AlwNI CAGNNNCTG 1 cut(s) 300
AoxI GGCC 3 cut(s) 85, 200, 618
ApeKI GCWGC 3 cut(s) 140, 341, 848
ApoI RAATTY 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 242, 838
AsuHPI GGTGA 2 cut(s) 157, 188
AsuNHI GCTAGC 1 cut(s) 823
AvaII GGWCC 2 cut(s) 242, 838
BanI GGYRCC 1 cut(s) 55
BarI GAAGNNNNNNTAC 2 cut(s) 856, 888
BbsI GAAGAC 1 cut(s) 536
BbvI GCAGC 3 cut(s) 127, 353, 835
BccI CCATC 4 cut(s) 228, 398, 510, 635
BciT130I CCWGG 4 cut(s) 60, 199, 765, 845
BciVI GTATCC 1 cut(s) 398
BclI TGATCA 1 cut(s) 561
BcoDI GTCTC 2 cut(s) 291, 441
BfaI CTAG 1 cut(s) 824
BfmI CTRYAG 3 cut(s) 292, 339, 589
BfuI GTATCC 1 cut(s) 398
BisI GCNGC 3 cut(s) 141, 342, 849
BlsI GCNGC 3 cut(s) 142, 343, 850
Bme1390I CCNGG 4 cut(s) 60, 199, 765, 845
Bme18I GGWCC 2 cut(s) 242, 838
BmeRI GACNNNNNGTC 1 cut(s) 696
BmgT120I GGNCC 2 cut(s) 242, 838
BmiI GGNNCC 2 cut(s) 57, 839
BmrFI CCNGG 4 cut(s) 60, 199, 765, 845
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 4 cut(s) 173, 351, 466, 886
BmtI GCTAGC 1 cut(s) 827
BmuI ACTGGG 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 536
BplI GAGNNNNNCTC 6 cut(s) 377, 409, 698, 730, 811, 843
BpmI CTGGAG 1 cut(s) 504
BsaBI GATNNNNATC 1 cut(s) 456
BsaWI WCCGGW 1 cut(s) 265
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 627
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 16, 487
Bse8I GATNNNNATC 1 cut(s) 456
BseBI CCWGG 4 cut(s) 60, 199, 765, 845
BseGI GGATG 2 cut(s) 457, 646
BseJI GATNNNNATC 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 550, 627
BseMII CTCAG 2 cut(s) 113, 408
BseNI ACTGG 2 cut(s) 16, 487
BseXI GCAGC 3 cut(s) 127, 353, 835
BshFI GGCC 3 cut(s) 87, 202, 620
BshNI GGYRCC 1 cut(s) 55
BsiSI CCGG 2 cut(s) 161, 266
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 550, 627
BsmAI GTCTC 2 cut(s) 291, 441
BsmFI GGGAC 1 cut(s) 22
BsnI GGCC 3 cut(s) 87, 202, 620
Bsp143I GATC 3 cut(s) 178, 561, 598
BspACI CCGC 3 cut(s) 143, 495, 841
BspANI GGCC 3 cut(s) 87, 202, 620
BspCNI CTCAG 2 cut(s) 114, 407
BspHI TCATGA 1 cut(s) 609
BspLI GGNNCC 2 cut(s) 57, 839
BspMAI CTGCAG 2 cut(s) 296, 343
BspOI GCTAGC 1 cut(s) 827
BspPI GGATC 1 cut(s) 606
BspT107I GGYRCC 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 16, 487
BssAI RCCGGY 1 cut(s) 160
BssMI GATC 3 cut(s) 178, 561, 598
Bst2UI CCWGG 4 cut(s) 60, 199, 765, 845
Bst4CI ACNGT 3 cut(s) 593, 663, 694
Bst6I CTCTTC 4 cut(s) 123, 376, 812, 877
BstAPI GCANNNNNTGC 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 711, 736, 825
BstDEI CTNAG 2 cut(s) 122, 394
BstEII GGTNACC 1 cut(s) 377
BstF5I GGATG 2 cut(s) 457, 646
BstKTI GATC 3 cut(s) 181, 564, 601
BstMAI GTCTC 2 cut(s) 291, 441
BstMBI GATC 3 cut(s) 178, 561, 598
BstMWI GCNNNNNNNGC 4 cut(s) 55, 84, 140, 300
BstNI CCWGG 4 cut(s) 60, 199, 765, 845
BstNSI RCATGY 3 cut(s) 473, 713, 738
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 4 cut(s) 58, 197, 763, 843
BstSFI CTRYAG 3 cut(s) 292, 339, 589
BstV1I GCAGC 3 cut(s) 127, 353, 835
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuI GTATCC 1 cut(s) 398
BsuRI GGCC 3 cut(s) 87, 202, 620
BtgZI GCGATG 1 cut(s) 247
BtsCI GGATG 2 cut(s) 457, 646
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 336, 480, 773
Cac8I GCNNGC 3 cut(s) 711, 736, 825
CaiI CAGNNNCTG 1 cut(s) 300
CciI TCATGA 1 cut(s) 609
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 242, 838
CviAII CATG 9 cut(s) 305, 383, 470, 545, 610, 666, 710, 735, 900
DdeI CTNAG 2 cut(s) 122, 394
DpnI GATC 3 cut(s) 180, 563, 600
DpnII GATC 3 cut(s) 178, 561, 598
DriI GACNNNNNGTC 1 cut(s) 696
Eam1104I CTCTTC 4 cut(s) 123, 376, 812, 877
Eam1105I GACNNNNNGTC 1 cut(s) 696
EarI CTCTTC 4 cut(s) 123, 376, 812, 877
EciI GGCGGA 1 cut(s) 830
Eco47I GGWCC 2 cut(s) 242, 838
Eco91I GGTNACC 1 cut(s) 377
EcoO65I GGTNACC 1 cut(s) 377
EcoRI GAATTC 1 cut(s) 63
EcoRII CCWGG 4 cut(s) 58, 197, 763, 843
EcoT22I ATGCAT 1 cut(s) 901
FaeI CATG 9 cut(s) 308, 386, 473, 548, 613, 669, 713, 738, 903
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 1 cut(s) 22
FatI CATG 9 cut(s) 304, 382, 469, 544, 609, 665, 709, 734, 899
FauI CCCGC 1 cut(s) 488
FbaI TGATCA 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 141, 342, 849
FokI GGATG 2 cut(s) 464, 653
Fsp4HI GCNGC 3 cut(s) 141, 342, 849
FspBI CTAG 1 cut(s) 824
GluI GCNGC 3 cut(s) 141, 342, 849
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 3 cut(s) 87, 202, 620
HapII CCGG 2 cut(s) 161, 266
Hin1II CATG 9 cut(s) 308, 386, 473, 548, 613, 669, 713, 738, 903
HincII GTYRAC 1 cut(s) 259
HindII GTYRAC 1 cut(s) 259
HinfI GANTC 6 cut(s) 211, 308, 386, 398, 535, 569
HpaII CCGG 2 cut(s) 161, 266
HphI GGTGA 2 cut(s) 157, 188
Hpy166II GTNNAC 2 cut(s) 24, 259
Hpy188I TCNGA 6 cut(s) 123, 397, 515, 690, 702, 718
Hpy188III TCNNGA 2 cut(s) 402, 610
Hpy8I GTNNAC 2 cut(s) 24, 259
HpyAV CCTTC 2 cut(s) 77, 485
HpyCH4III ACNGT 3 cut(s) 593, 663, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 84, 140, 300
HpyF3I CTNAG 2 cut(s) 122, 394
Hsp92II CATG 9 cut(s) 308, 386, 473, 548, 613, 669, 713, 738, 903
Ksp22I TGATCA 1 cut(s) 561
Kzo9I GATC 3 cut(s) 178, 561, 598
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 3 cut(s) 127, 353, 835
LweI GCATC 4 cut(s) 173, 351, 466, 886
MaeI CTAG 1 cut(s) 824
MaeIII GTNAC 3 cut(s) 278, 334, 377
MalI GATC 3 cut(s) 180, 563, 600
MboI GATC 3 cut(s) 178, 561, 598
MboII GAAGA 6 cut(s) 140, 363, 541, 682, 829, 864
MflI RGATCY 1 cut(s) 598
MluCI AATT 3 cut(s) 63, 96, 888
MlyI GAGTC 2 cut(s) 302, 395
MnlI CCTC 8 cut(s) 24, 106, 124, 412, 724, 803, 813, 866
Mph1103I ATGCAT 1 cut(s) 901
MseI TTAA 2 cut(s) 273, 413
MspI CCGG 2 cut(s) 161, 266
MspR9I CCNGG 4 cut(s) 60, 199, 765, 845
MvaI CCWGG 4 cut(s) 60, 199, 765, 845
MwoI GCNNNNNNNGC 4 cut(s) 55, 84, 140, 300
NdeII GATC 3 cut(s) 178, 561, 598
NheI GCTAGC 1 cut(s) 823
NlaIII CATG 9 cut(s) 308, 386, 473, 548, 613, 669, 713, 738, 903
NlaIV GGNNCC 2 cut(s) 57, 839
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 3 cut(s) 473, 713, 738
PaeI GCATGC 2 cut(s) 713, 738
PagI TCATGA 1 cut(s) 609
PciI ACATGT 1 cut(s) 469
PfeI GAWTC 4 cut(s) 211, 398, 535, 569
PkrI GCNGC 3 cut(s) 142, 343, 850
PleI GAGTC 2 cut(s) 302, 394
PpsI GAGTC 2 cut(s) 302, 394
PscI ACATGT 1 cut(s) 469
Psp6I CCWGG 4 cut(s) 58, 197, 763, 843
PspEI GGTNACC 1 cut(s) 377
PspGI CCWGG 4 cut(s) 58, 197, 763, 843
PspN4I GGNNCC 2 cut(s) 57, 839
PspPI GGNCC 2 cut(s) 242, 838
PstI CTGCAG 2 cut(s) 296, 343
PstNI CAGNNNCTG 1 cut(s) 300
PsuI RGATCY 1 cut(s) 598
SaqAI TTAA 2 cut(s) 273, 413
SatI GCNGC 3 cut(s) 141, 342, 849
Sau3AI GATC 3 cut(s) 178, 561, 598
Sau96I GGNCC 2 cut(s) 242, 838
SchI GAGTC 2 cut(s) 302, 395
ScrFI CCNGG 4 cut(s) 60, 199, 765, 845
SetI ASST 6 cut(s) 178, 224, 332, 766, 808, 829
SfaNI GCATC 4 cut(s) 173, 351, 466, 886
SfcI CTRYAG 3 cut(s) 292, 339, 589
SinI GGWCC 2 cut(s) 242, 838
SphI GCATGC 2 cut(s) 713, 738
Sse9I AATT 3 cut(s) 63, 96, 888
SsiI CCGC 3 cut(s) 143, 495, 841
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 824
StyD4I CCNGG 4 cut(s) 58, 197, 763, 843
TaaI ACNGT 3 cut(s) 593, 663, 694
TaqI TCGA 1 cut(s) 359
TasI AATT 3 cut(s) 63, 96, 888
TfiI GAWTC 4 cut(s) 211, 398, 535, 569
Tru1I TTAA 2 cut(s) 273, 413
Tru9I TTAA 2 cut(s) 273, 413
TscAI CASTG 3 cut(s) 343, 487, 780
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 140, 341, 848
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 504
TspGWI ACGGA 1 cut(s) 653
TspRI CASTG 3 cut(s) 343, 487, 780
VpaK11BI GGWCC 2 cut(s) 242, 838
XapI RAATTY 1 cut(s) 63
XceI RCATGY 3 cut(s) 473, 713, 738
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.