AT4G21870

heat- shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014398
Physical Location & Seq
Reverse (-)
11603481 .. 11604637
1157 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21870.1

Sequence Viewer

Length: 405 bp
ATGGATTTTCAGACAATTCAAGTGATGCCATGGGAATATGTTCTAGCTTCTCAATCTCTTAATAACTATCAAGAGAATCATGTTCGTTGGTCTCAGTCTCCAGATTCTCACACTTTCTCTGTTGATCTTCCTGGGTTAAGGAAAGAAGAAATAAAAGTTGAGATCGAAGATTCGATATACTTAATCATACGAACGGAGGCAACCCCTATGTCGCCTCCGGATCAGCCTTTGAAGACTTTTAAGAGGAAATTTCGGTTGCCGGAATCGATAGATATGATCGGAATATCAGCTGGCTACGAAGATGGTGTGTTGACTGTGATTGTACCCAAGAGGATTATGACAAGGAGGCTCATTGATCCTTCTGATGTTCCTGAAAGTCTTCAACTTCTTGCTAGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

134

Amino Acids

15.39

Weight (kDa)

5.17

Isoelectric Point (pI)

73.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 30 - 112 3.7e-15 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 217
AclWI GGATC 2 cut(s) 228, 350
AcsI RAATTY 1 cut(s) 248
AfaI GTAC 1 cut(s) 324
AgsI TTSAA 3 cut(s) 20, 232, 383
AjnI CCWGG 1 cut(s) 130
AluBI AGCT 3 cut(s) 47, 290, 398
AluI AGCT 3 cut(s) 47, 290, 398
Alw26I GTCTC 2 cut(s) 96, 102
AlwI GGATC 2 cut(s) 228, 350
Aor13HI TCCGGA 1 cut(s) 217
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 1 cut(s) 248
Asp700I GAANNNNTTC 2 cut(s) 39, 378
BbsI GAAGAC 2 cut(s) 239, 371
BbvI GCAGC 1 cut(s) 385
BccI CCATC 1 cut(s) 296
BciT130I CCWGG 1 cut(s) 132
BcoDI GTCTC 2 cut(s) 96, 102
BfaI CTAG 2 cut(s) 44, 393
BisI GCNGC 1 cut(s) 399
BlsI GCNGC 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 1 cut(s) 15
BpiI GAAGAC 2 cut(s) 239, 371
BpmI CTGGAG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 266
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 29, 131
BsaWI WCCGGW 1 cut(s) 217
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 1 cut(s) 132
BseCI ATCGAT 1 cut(s) 266
BseDI CCNNGG 2 cut(s) 29, 131
BseMII CTCAG 1 cut(s) 107
BseXI GCAGC 1 cut(s) 385
BshVI ATCGAT 1 cut(s) 266
BsiSI CCGG 2 cut(s) 218, 260
BsmAI GTCTC 2 cut(s) 96, 102
Bso31I GGTCTC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 5 cut(s) 124, 162, 220, 276, 355
Bsp19I CCATGG 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 106
BspDI ATCGAT 1 cut(s) 266
BspEI TCCGGA 1 cut(s) 217
BspPI GGATC 2 cut(s) 228, 350
BspTNI GGTCTC 1 cut(s) 96
BssECI CCNNGG 2 cut(s) 29, 131
BssMI GATC 5 cut(s) 124, 162, 220, 276, 355
BssT1I CCWWGG 1 cut(s) 29
Bst2UI CCWGG 1 cut(s) 132
Bst4CI ACNGT 1 cut(s) 316
BstC8I GCNNGC 1 cut(s) 292
BstDEI CTNAG 1 cut(s) 93
BstDSI CCRYGG 1 cut(s) 29
BstKTI GATC 5 cut(s) 127, 165, 223, 279, 358
BstMAI GTCTC 2 cut(s) 96, 102
BstMBI GATC 5 cut(s) 124, 162, 220, 276, 355
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstNI CCWGG 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 130
BstV1I GCAGC 1 cut(s) 385
BstV2I GAAGAC 2 cut(s) 239, 371
Bsu15I ATCGAT 1 cut(s) 266
BsuTUI ATCGAT 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 292
ClaI ATCGAT 1 cut(s) 266
Csp6I GTAC 1 cut(s) 323
CviAII CATG 2 cut(s) 30, 80
CviJI RGCY 6 cut(s) 47, 226, 290, 294, 349, 398
CviKI_1 RGCY 6 cut(s) 47, 226, 290, 294, 349, 398
CviQI GTAC 1 cut(s) 323
DdeI CTNAG 1 cut(s) 93
DpnI GATC 5 cut(s) 126, 164, 222, 278, 357
DpnII GATC 5 cut(s) 124, 162, 220, 276, 355
Eco130I CCWWGG 1 cut(s) 29
Eco31I GGTCTC 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 29
FaeI CATG 2 cut(s) 33, 83
FaiI YATR 8 cut(s) 31, 39, 81, 178, 188, 209, 275, 338
FatI CATG 2 cut(s) 29, 79
Fnu4HI GCNGC 1 cut(s) 399
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 2 cut(s) 44, 393
GluI GCNGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 84
HapII CCGG 2 cut(s) 218, 260
Hin1II CATG 2 cut(s) 33, 83
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HinfI GANTC 4 cut(s) 76, 104, 170, 263
HpaII CCGG 2 cut(s) 218, 260
Hpy166II GTNNAC 1 cut(s) 312
Hpy188I TCNGA 3 cut(s) 12, 281, 364
Hpy188III TCNNGA 4 cut(s) 71, 101, 218, 371
Hpy8I GTNNAC 1 cut(s) 312
HpyAV CCTTC 1 cut(s) 369
HpyCH4III ACNGT 1 cut(s) 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
HpyF3I CTNAG 1 cut(s) 93
Hsp92II CATG 2 cut(s) 33, 83
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 5 cut(s) 124, 162, 220, 276, 355
LpnPI CCDG 7 cut(s) 114, 117, 144, 231, 273, 276, 384
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 1 cut(s) 15
MaeI CTAG 2 cut(s) 44, 393
MalI GATC 5 cut(s) 126, 164, 222, 278, 357
MboI GATC 5 cut(s) 124, 162, 220, 276, 355
MboII GAAGA 6 cut(s) 119, 158, 179, 244, 311, 371
MluCI AATT 2 cut(s) 15, 248
MnlI CCTC 5 cut(s) 190, 225, 237, 324, 339
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 2 cut(s) 39, 378
MseI TTAA 5 cut(s) 60, 137, 182, 240, 403
MspA1I CMGCKG 1 cut(s) 290
MspI CCGG 2 cut(s) 218, 260
MspR9I CCNGG 1 cut(s) 132
MvaI CCWGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 398
NcoI CCATGG 1 cut(s) 29
NdeII GATC 5 cut(s) 124, 162, 220, 276, 355
NlaIII CATG 2 cut(s) 33, 83
PdmI GAANNNNTTC 2 cut(s) 39, 378
PfeI GAWTC 4 cut(s) 76, 104, 170, 263
PkrI GCNGC 1 cut(s) 400
Psp6I CCWGG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 1 cut(s) 324
RsaNI GTAC 1 cut(s) 323
SaqAI TTAA 5 cut(s) 60, 137, 182, 240, 403
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 5 cut(s) 124, 162, 220, 276, 355
ScrFI CCNGG 1 cut(s) 132
SetI ASST 3 cut(s) 49, 292, 400
SfaNI GCATC 1 cut(s) 15
Sse9I AATT 2 cut(s) 15, 248
SspMI CTAG 2 cut(s) 44, 393
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 316
TaqI TCGA 3 cut(s) 165, 173, 266
TasI AATT 2 cut(s) 15, 248
TfiI GAWTC 4 cut(s) 76, 104, 170, 263
Tru1I TTAA 5 cut(s) 60, 137, 182, 240, 403
Tru9I TTAA 5 cut(s) 60, 137, 182, 240, 403
TseI GCWGC 1 cut(s) 398
TspGWI ACGGA 1 cut(s) 209
XapI RAATTY 1 cut(s) 248
XmnI GAANNNNTTC 2 cut(s) 39, 378
XspI CTAG 2 cut(s) 44, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.