Rh5DG030900

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014398
Physical Location & Seq
Forward (+)
2197899 .. 2198409
511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG030900.1

Sequence Viewer

Length: 429 bp
ATGGAGCTCCCAAACCAAAACTTCCAGTTTTACCAATATGTCTTCCCCTCTCATCTTGTTTATCCATATCACTTGACCCCAGAAAACTATGTGCACTGGACTGAAACCCCAGAGTCTCACATTTTCTCAGCTGACCTCCCTGGTGTGAGGAAGGAGGAGATAAAAGTAGAAGTGGAGGACTCAATATACCTGATAATCAGAACACAGAGACTTGATGATGATGGTGATGGTGATGAGGAGTCCACAGAGGCTAGGAGGACCTTCACGAGGAAGTTTCGGCTTCCGGGTATGATCGATATCGAAAGAATCTCAGCTGCATATGAAGATGGTGTGTTGACAATCACAGTGCCAAGGTCATTCAGGAGGAGTTTCTTTATTGAACCAGCTGACATGCCTGAAAGGCTTGATGTTCTTGCAAGAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

142

Amino Acids

16.67

Weight (kDa)

4.81

Isoelectric Point (pI)

63.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 119 4.3e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 5 cut(s) 7, 131, 314, 386, 422
AluI AGCT 5 cut(s) 7, 131, 314, 386, 422
Alw21I GWGCWC 2 cut(s) 9, 96
Alw26I GTCTC 2 cut(s) 120, 202
Alw44I GTGCAC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 314, 422
AspS9I GGNCC 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 2 cut(s) 236, 242
AvaII GGWCC 1 cut(s) 258
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 265
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 9, 96
BbvI GCAGC 2 cut(s) 301, 409
BccI CCATC 3 cut(s) 215, 221, 320
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 2 cut(s) 120, 202
BfaI CTAG 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 400
BisI GCNGC 2 cut(s) 315, 423
BlsI GCNGC 2 cut(s) 316, 424
Bme1390I CCNGG 2 cut(s) 141, 285
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmrFI CCNGG 2 cut(s) 141, 285
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 285
Bsa29I ATCGAT 1 cut(s) 294
BsaBI GATNNNNATC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 139, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 25, 101
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 1 cut(s) 141
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 139, 350
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMII CTCAG 2 cut(s) 141, 324
BseNI ACTGG 2 cut(s) 25, 101
BseRI GAGGAG 3 cut(s) 170, 251, 379
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 301, 409
BshVI ATCGAT 1 cut(s) 294
BsiHKAI GWGCWC 2 cut(s) 9, 96
BsiSI CCGG 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 2 cut(s) 120, 202
Bsp1286I GDGCHC 2 cut(s) 9, 96
Bsp143I GATC 1 cut(s) 291
BspCNI CTCAG 2 cut(s) 140, 323
BspDI ATCGAT 1 cut(s) 294
BsrI ACTGG 2 cut(s) 25, 101
BssECI CCNNGG 2 cut(s) 139, 350
BssMI GATC 1 cut(s) 291
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 1 cut(s) 350
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 422
BstDEI CTNAG 2 cut(s) 127, 310
BstENI CCTNNNNNAGG 1 cut(s) 265
BstKTI GATC 1 cut(s) 294
BstMAI GTCTC 2 cut(s) 120, 202
BstMBI GATC 1 cut(s) 291
BstMWI GCNNNNNNNGC 2 cut(s) 400, 422
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 2 cut(s) 139, 283
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 301, 409
BstV2I GAAGAC 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 94, 351
Cfr13I GGNCC 1 cut(s) 258
ClaI ATCGAT 1 cut(s) 294
CviAII CATG 1 cut(s) 391
CviJI RGCY 8 cut(s) 7, 131, 251, 280, 314, 386, 403, 422
CviKI_1 RGCY 8 cut(s) 7, 131, 251, 280, 314, 386, 403, 422
DdeI CTNAG 2 cut(s) 127, 310
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 258
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 258
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 350
FaeI CATG 1 cut(s) 394
FaiI YATR 8 cut(s) 39, 67, 90, 187, 290, 319, 321, 392
FalI AAGNNNNNCTT 1 cut(s) 409
FatI CATG 1 cut(s) 390
FauNDI CATATG 1 cut(s) 319
Fnu4HI GCNGC 2 cut(s) 315, 423
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 315, 423
FspBI CTAG 1 cut(s) 252
GluI GCNGC 2 cut(s) 315, 423
HapII CCGG 1 cut(s) 284
Hin1II CATG 1 cut(s) 394
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 4 cut(s) 113, 179, 239, 306
HpaII CCGG 1 cut(s) 284
HphI GGTGA 2 cut(s) 236, 242
Hpy166II GTNNAC 3 cut(s) 94, 243, 336
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 2 cut(s) 265, 361
Hpy8I GTNNAC 3 cut(s) 94, 243, 336
HpyAV CCTTC 2 cut(s) 145, 271
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4V TGCA 3 cut(s) 94, 317, 416
HpyF10VI GCNNNNNNNGC 2 cut(s) 400, 422
HpyF3I CTNAG 2 cut(s) 127, 310
Hsp92II CATG 1 cut(s) 394
Kzo9I GATC 1 cut(s) 291
LmnI GCTCC 2 cut(s) 4, 12
Lsp1109I GCAGC 2 cut(s) 301, 409
MaeI CTAG 1 cut(s) 252
MalI GATC 1 cut(s) 293
MboI GATC 1 cut(s) 291
MboII GAAGA 2 cut(s) 34, 335
MhlI GDGCHC 2 cut(s) 9, 96
MlyI GAGTC 3 cut(s) 122, 173, 248
MspA1I CMGCKG 3 cut(s) 131, 314, 386
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 2 cut(s) 141, 285
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 400, 422
NciI CCSGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 319
NdeII GATC 1 cut(s) 291
NlaIII CATG 1 cut(s) 394
NspI RCATGY 1 cut(s) 394
PfeI GAWTC 1 cut(s) 306
PkrI GCNGC 2 cut(s) 316, 424
PleI GAGTC 3 cut(s) 121, 173, 247
PpsI GAGTC 3 cut(s) 121, 173, 247
PpuMI RGGWCCY 1 cut(s) 258
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 258
PspPPI RGGWCCY 1 cut(s) 258
PvuII CAGCTG 3 cut(s) 131, 314, 386
SacI GAGCTC 1 cut(s) 9
SatI GCNGC 2 cut(s) 315, 423
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 1 cut(s) 258
SchI GAGTC 3 cut(s) 122, 173, 248
ScrFI CCNGG 2 cut(s) 141, 285
SduI GDGCHC 2 cut(s) 9, 96
SetI ASST 9 cut(s) 9, 133, 138, 192, 263, 316, 356, 388, 424
SinI GGWCC 1 cut(s) 258
SspMI CTAG 1 cut(s) 252
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 139, 283
StyI CCWWGG 1 cut(s) 350
TaaI ACNGT 1 cut(s) 346
TaqI TCGA 2 cut(s) 294, 300
TfiI GAWTC 1 cut(s) 306
TscAI CASTG 2 cut(s) 101, 351
TseI GCWGC 2 cut(s) 314, 422
TspDTI ATGAA 1 cut(s) 336
TspRI CASTG 2 cut(s) 101, 351
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 258
XagI CCTNNNNNAGG 1 cut(s) 265
XceI RCATGY 1 cut(s) 394
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.