MD05G1343700.v1.1

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014398
Physical Location & Seq
Reverse (-)
46361886 .. 46362915
1030 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1343700.v1.1.491

Sequence Viewer

Length: 414 bp
ATGGAGATCCCAGCCTTCCACCATTACCAATATTTCTTCCCTTCTCACCTCATCTACCCATATCATTTGGCACCAGAAAACTATTTGCACTGGACTGAGACCCCTGTTTCTCACATTTTCTCCGTTGACCTCCCCGGTGTGAGGAAAGAGGAGATAAAAGTACAAGTGGAGGACTCAGCATACCTTATAATTCGAACACAGAGGATTGAGGAGGAGGAGGAGTCCACAGAGCAGCCTAGCAGGACCTTCATGAGGAAGTTCCGGATTCCGGGTCAGGCCGATATCGAACGGATTTCAGCCAGATACGAAGCCGGAGTGTTGACGGTCACAGTGCCGAGATCTTTGAGGAGGGCTTTCTTCATTGAGCCAGCTGATGTGCCAGAGAGGCTTCAAGTTCTTGCAAGGGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

138

Amino Acids

16.11

Weight (kDa)

5.86

Isoelectric Point (pI)

73.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 115 1.3e-17 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 70
AccIII TCCGGA 1 cut(s) 261
AfaI GTAC 1 cut(s) 162
AfiI CCNNNNNNNGG 3 cut(s) 141, 252, 268
AgsI TTSAA 1 cut(s) 392
AluBI AGCT 1 cut(s) 371
AluI AGCT 1 cut(s) 371
Alw26I GTCTC 1 cut(s) 92
Aor13HI TCCGGA 1 cut(s) 261
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 2 cut(s) 232, 407
AspS9I GGNCC 1 cut(s) 243
AsuC2I CCSGG 2 cut(s) 135, 270
AsuHPI GGTGA 1 cut(s) 38
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 243
BanI GGYRCC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 244, 394
BcnI CCSGG 2 cut(s) 135, 270
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 237
BglI GCCNNNNNGGC 1 cut(s) 385
BglII AGATCT 1 cut(s) 338
BisI GCNGC 2 cut(s) 233, 408
BlsI GCNGC 2 cut(s) 234, 409
Bme1390I CCNGG 2 cut(s) 135, 270
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 2 cut(s) 135, 270
Bpu14I TTCGAA 1 cut(s) 193
BpuMI CCSGG 2 cut(s) 135, 270
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 261
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 206, 236
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 252, 268
Bse1I ACTGG 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 141, 252, 268
BseMII CTCAG 2 cut(s) 87, 189
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 6 cut(s) 164, 224, 227, 230, 233, 361
BseXI GCAGC 2 cut(s) 244, 394
BseYI CCCAGC 1 cut(s) 10
BshFI GGCC 1 cut(s) 278
BshNI GGYRCC 1 cut(s) 70
BsiSI CCGG 4 cut(s) 135, 262, 269, 312
BslI CCNNNNNNNGG 3 cut(s) 141, 252, 268
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 1 cut(s) 278
Bso31I GGTCTC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 193
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 2 cut(s) 6, 338
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 2 cut(s) 88, 188
BspEI TCCGGA 1 cut(s) 261
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 72
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 70
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 2 cut(s) 6, 338
Bst4CI ACNGT 2 cut(s) 325, 331
BstAPI GCANNNNNTGC 1 cut(s) 407
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 2 cut(s) 96, 175
BstENI CCTNNNNNAGG 1 cut(s) 250
BstKTI GATC 2 cut(s) 9, 341
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 2 cut(s) 6, 338
BstMWI GCNNNNNNNGC 2 cut(s) 385, 407
BstSCI CCNGG 2 cut(s) 133, 268
BstV1I GCAGC 2 cut(s) 244, 394
BstX2I RGATCY 2 cut(s) 6, 338
BstYI RGATCY 2 cut(s) 6, 338
BsuRI GGCC 1 cut(s) 278
BtsIMutI CAGTG 2 cut(s) 88, 336
Cac8I GCNNGC 1 cut(s) 369
CciI TCATGA 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 243
Csp6I GTAC 1 cut(s) 161
CviAII CATG 1 cut(s) 250
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 2 cut(s) 96, 175
DpnI GATC 2 cut(s) 8, 340
DpnII GATC 2 cut(s) 6, 338
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 243
EcoNI CCTNNNNNAGG 1 cut(s) 250
EcoO109I RGGNCCY 1 cut(s) 243
EcoRV GATATC 1 cut(s) 283
FaeI CATG 1 cut(s) 253
FaiI YATR 4 cut(s) 61, 181, 188, 251
FalI AAGNNNNNCTT 1 cut(s) 394
FatI CATG 1 cut(s) 249
Fnu4HI GCNGC 2 cut(s) 233, 408
Fsp4HI GCNGC 2 cut(s) 233, 408
FspBI CTAG 1 cut(s) 237
GluI GCNGC 2 cut(s) 233, 408
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 1 cut(s) 278
HapII CCGG 4 cut(s) 135, 262, 269, 312
Hin1II CATG 1 cut(s) 253
HincII GTYRAC 2 cut(s) 127, 321
HindII GTYRAC 2 cut(s) 127, 321
HinfI GANTC 3 cut(s) 173, 221, 265
HpaII CCGG 4 cut(s) 135, 262, 269, 312
HphI GGTGA 1 cut(s) 38
Hpy166II GTNNAC 3 cut(s) 127, 225, 321
Hpy188III TCNNGA 2 cut(s) 250, 262
Hpy8I GTNNAC 3 cut(s) 127, 225, 321
HpyAV CCTTC 3 cut(s) 25, 51, 256
HpyCH4III ACNGT 2 cut(s) 325, 331
HpyCH4V TGCA 2 cut(s) 88, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 385, 407
HpyF3I CTNAG 2 cut(s) 96, 175
Hsp92II CATG 1 cut(s) 253
Kpn2I TCCGGA 1 cut(s) 261
Kzo9I GATC 2 cut(s) 6, 338
Lsp1109I GCAGC 2 cut(s) 244, 394
MaeI CTAG 1 cut(s) 237
MaeIII GTNAC 1 cut(s) 325
MalI GATC 2 cut(s) 8, 340
MboI GATC 2 cut(s) 6, 338
MboII GAAGA 2 cut(s) 28, 349
MflI RGATCY 2 cut(s) 6, 338
MluCI AATT 1 cut(s) 189
MlyI GAGTC 2 cut(s) 167, 230
MroI TCCGGA 1 cut(s) 261
MseI TTAA 1 cut(s) 412
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 4 cut(s) 135, 262, 269, 312
MspR9I CCNGG 2 cut(s) 135, 270
MwoI GCNNNNNNNGC 2 cut(s) 385, 407
NciI CCSGG 2 cut(s) 135, 270
NdeII GATC 2 cut(s) 6, 338
NlaIII CATG 1 cut(s) 253
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 325
NspV TTCGAA 1 cut(s) 193
PagI TCATGA 1 cut(s) 249
PfeI GAWTC 1 cut(s) 265
PkrI GCNGC 2 cut(s) 234, 409
PleI GAGTC 2 cut(s) 167, 229
PpsI GAGTC 2 cut(s) 167, 229
PpuMI RGGWCCY 1 cut(s) 243
PsiI TTATAA 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 1 cut(s) 243
PspPPI RGGWCCY 1 cut(s) 243
PsuI RGATCY 2 cut(s) 6, 338
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 412
SatI GCNGC 2 cut(s) 233, 408
Sau3AI GATC 2 cut(s) 6, 338
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 2 cut(s) 167, 230
ScrFI CCNGG 2 cut(s) 135, 270
SetI ASST 5 cut(s) 51, 132, 186, 248, 373
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 1 cut(s) 243
Sse9I AATT 1 cut(s) 189
SspI AATATT 1 cut(s) 32
SspMI CTAG 1 cut(s) 237
StyD4I CCNGG 2 cut(s) 133, 268
TaaI ACNGT 2 cut(s) 325, 331
TaqI TCGA 2 cut(s) 193, 285
TasI AATT 1 cut(s) 189
TatI WGTACW 1 cut(s) 160
TfiI GAWTC 1 cut(s) 265
Tru1I TTAA 1 cut(s) 412
Tru9I TTAA 1 cut(s) 412
TscAI CASTG 2 cut(s) 95, 336
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 2 cut(s) 232, 407
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 2 cut(s) 238, 349
TspGWI ACGGA 2 cut(s) 112, 304
TspRI CASTG 2 cut(s) 95, 336
VpaK11BI GGWCC 1 cut(s) 243
XagI CCTNNNNNAGG 1 cut(s) 250
XspI CTAG 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.