FvH4_3g02330

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014398
Physical Location & Seq
Forward (+)
1182925 .. 1183445
521 bp
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UTR
Exon/CDS
Intron
FvH4_3g02330.t1

Sequence Viewer

Length: 426 bp
ATGGAGCTACCAAACCAAAACTTTCAGCTTTACCAGTATGTCTTCCCCTCTCATCTTGTTTATCCATATCACTTAGCCCCAGAAAACTATGTGCACTGGACTGAAACCCCAGAAACTCACATTTTCTCAGCTGATCTCCCTGGTGTGAGGAAGGAGGAGATAAAGGTGGAAGTGGAGGACTCAATATACCTGATAATCAGAACACACAGACTTGATGATGGTGATGGTGATGAGTCTTCCACAGAGGCTAGGAGGACCTTCATGAGAAAGTTTCGGCTTCCAAGTCAAGTCGACATCGAGAGAATCTCAGCTGGATATGAAGATGGAATCTTGACAATCACAGTGCCAAGGTTGTTGAGGAGGAGTTTCTTCATTGAACCAGCTGACATGCCAAAAAGGCTTGAAGTTCTTGCAAGAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

142

Amino Acids

16.52

Weight (kDa)

5.36

Isoelectric Point (pI)

61.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 118 1.3e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 291
AgsI TTSAA 2 cut(s) 377, 404
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 6 cut(s) 7, 28, 131, 311, 383, 419
AluI AGCT 6 cut(s) 7, 28, 131, 311, 383, 419
Alw21I GWGCWC 1 cut(s) 96
Alw44I GTGCAC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 2 cut(s) 233, 239
AvaII GGWCC 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 96
BbsI GAAGAC 2 cut(s) 34, 228
Bbv12I GWGCWC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 406
BccI CCATC 3 cut(s) 212, 218, 317
BciT130I CCWGG 1 cut(s) 141
BfaI CTAG 1 cut(s) 249
BglI GCCNNNNNGGC 1 cut(s) 397
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 1 cut(s) 255
BmgT120I GGNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 141
BpiI GAAGAC 2 cut(s) 34, 228
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BsaJI CCNNGG 2 cut(s) 139, 347
Bse1I ACTGG 2 cut(s) 34, 101
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 139, 347
BseMII CTCAG 2 cut(s) 141, 321
BseNI ACTGG 2 cut(s) 34, 101
BseRI GAGGAG 3 cut(s) 170, 373, 376
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 96
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 140, 320
BspHI TCATGA 1 cut(s) 261
BsrI ACTGG 2 cut(s) 34, 101
BssECI CCNNGG 2 cut(s) 139, 347
BssMI GATC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 347
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 343
BstAPI GCANNNNNTGC 1 cut(s) 419
BstDEI CTNAG 3 cut(s) 73, 127, 307
BstKTI GATC 1 cut(s) 136
BstMBI GATC 1 cut(s) 133
BstMWI GCNNNNNNNGC 2 cut(s) 397, 419
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 406
BstV2I GAAGAC 2 cut(s) 34, 228
BtsIMutI CAGTG 2 cut(s) 94, 348
CciI TCATGA 1 cut(s) 261
Cfr13I GGNCC 1 cut(s) 255
CviAII CATG 2 cut(s) 262, 388
DdeI CTNAG 3 cut(s) 73, 127, 307
DpnI GATC 1 cut(s) 135
DpnII GATC 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 255
EcoO109I RGGNCCY 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 2 cut(s) 265, 391
FaiI YATR 7 cut(s) 39, 67, 90, 187, 263, 318, 389
FalI AAGNNNNNCTT 1 cut(s) 406
FatI CATG 2 cut(s) 261, 387
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 1 cut(s) 420
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 1 cut(s) 249
GluI GCNGC 1 cut(s) 420
Hin1II CATG 2 cut(s) 265, 391
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 4 cut(s) 179, 233, 303, 327
HphI GGTGA 2 cut(s) 233, 239
Hpy166II GTNNAC 2 cut(s) 94, 292
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 3 cut(s) 262, 298, 331
Hpy8I GTNNAC 2 cut(s) 94, 292
HpyAV CCTTC 2 cut(s) 145, 268
HpyCH4III ACNGT 1 cut(s) 343
HpyCH4V TGCA 2 cut(s) 94, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 397, 419
HpyF3I CTNAG 3 cut(s) 73, 127, 307
Hsp92II CATG 2 cut(s) 265, 391
Kzo9I GATC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 4
LpnPI CCDG 9 cut(s) 47, 82, 93, 123, 126, 153, 203, 297, 393
Lsp1109I GCAGC 1 cut(s) 406
MaeI CTAG 1 cut(s) 249
MalI GATC 1 cut(s) 135
MboI GATC 1 cut(s) 133
MboII GAAGA 4 cut(s) 34, 228, 332, 361
MhlI GDGCHC 1 cut(s) 96
MlyI GAGTC 2 cut(s) 173, 242
MnlI CCTC 8 cut(s) 58, 141, 148, 169, 238, 246, 351, 354
MspA1I CMGCKG 3 cut(s) 131, 311, 383
MspR9I CCNGG 1 cut(s) 141
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 397, 419
NdeII GATC 1 cut(s) 133
NlaIII CATG 2 cut(s) 265, 391
NspI RCATGY 1 cut(s) 391
PagI TCATGA 1 cut(s) 261
PfeI GAWTC 2 cut(s) 303, 327
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 2 cut(s) 173, 241
PpsI GAGTC 2 cut(s) 173, 241
PpuMI RGGWCCY 1 cut(s) 255
Psp5II RGGWCCY 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 255
PspPPI RGGWCCY 1 cut(s) 255
PvuII CAGCTG 3 cut(s) 131, 311, 383
SalI GTCGAC 1 cut(s) 290
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 1 cut(s) 133
Sau96I GGNCC 1 cut(s) 255
SchI GAGTC 2 cut(s) 173, 242
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 96
SinI GGWCC 1 cut(s) 255
SspMI CTAG 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 139
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 1 cut(s) 343
TaqI TCGA 2 cut(s) 291, 297
TfiI GAWTC 2 cut(s) 303, 327
TscAI CASTG 2 cut(s) 101, 348
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 3 cut(s) 250, 333, 361
TspRI CASTG 2 cut(s) 101, 348
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 255
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.