RchiOBHm_Chr5g0003551

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014398
Physical Location & Seq
Forward (+)
2226328 .. 2227774
1447 bp
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UTR
Exon/CDS
Intron
PRQ28486

Sequence Viewer

Length: 420 bp
ATGGAGCTCCCAAACCAAAACTTCCAGCTTTACCAATATGTCTTCCCCTCTCATCTTGTTTATCCATATCACTTGACCCCAGAAAACTATGTGCACTGGACTGAAACCCCTGAGTCTCACATTTTCTCAGCTGACCTCCCTGGTGTGAGGAAGGAGGAGATAAAAGTGGAAGTGGAGGACTCAATATACCTGATAATCAGAACACAGAGACTTGATGATGGTGATGAGGAGTCCACAGAGGCTAGGAGGACCTTCACGAGGAAGTTTCGGCTTCCGGGTACGGTCGATATCGAAAGAATCTCAGCTGCATATGAAGATGGTGTGTTGACAATCACAGTGCCAAGGTCATTGAGGAGGAGTTTCTTTATTGAACCAGCTGACATGCCTGAAAGGCTTGATGTTCTTGCAAGAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

139

Amino Acids

16.27

Weight (kDa)

4.98

Isoelectric Point (pI)

62.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 117 2.5e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 1 cut(s) 371
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 6 cut(s) 7, 28, 131, 305, 377, 413
AluI AGCT 6 cut(s) 7, 28, 131, 305, 377, 413
Alw21I GWGCWC 2 cut(s) 9, 96
Alw26I GTCTC 2 cut(s) 120, 202
Alw44I GTGCAC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 305, 413
AspS9I GGNCC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 249
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 9, 96
BbvI GCAGC 2 cut(s) 292, 400
BccI CCATC 2 cut(s) 212, 311
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 120, 202
BfaI CTAG 1 cut(s) 243
BglI GCCNNNNNGGC 1 cut(s) 391
BisI GCNGC 2 cut(s) 306, 414
BlsI GCNGC 2 cut(s) 307, 415
Bme1390I CCNGG 2 cut(s) 141, 276
Bme18I GGWCC 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 249
BmrFI CCNGG 2 cut(s) 141, 276
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 139, 341
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 139, 341
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 3 cut(s) 102, 141, 315
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 4 cut(s) 170, 242, 367, 370
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 292, 400
Bsh1285I CGRYCG 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 9, 96
BsiSI CCGG 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 2 cut(s) 120, 202
Bsp1286I GDGCHC 2 cut(s) 9, 96
BspCNI CTCAG 3 cut(s) 103, 140, 314
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 139, 341
BssSI CACGAG 1 cut(s) 256
BssT1I CCWWGG 1 cut(s) 341
Bst2BI CACGAG 1 cut(s) 256
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 2 cut(s) 283, 337
BstAPI GCANNNNNTGC 1 cut(s) 413
BstDEI CTNAG 3 cut(s) 111, 127, 301
BstENI CCTNNNNNAGG 1 cut(s) 256
BstMAI GTCTC 2 cut(s) 120, 202
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 2 cut(s) 391, 413
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 139, 274
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 292, 400
BstV2I GAAGAC 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 94, 342
Cfr13I GGNCC 1 cut(s) 249
Csp6I GTAC 1 cut(s) 279
CviAII CATG 1 cut(s) 382
CviJI RGCY 9 cut(s) 7, 28, 131, 242, 271, 305, 377, 394, 413
CviKI_1 RGCY 9 cut(s) 7, 28, 131, 242, 271, 305, 377, 394, 413
CviQI GTAC 1 cut(s) 279
DdeI CTNAG 3 cut(s) 111, 127, 301
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 256
EcoO109I RGGNCCY 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 341
FaeI CATG 1 cut(s) 385
FaiI YATR 7 cut(s) 39, 67, 90, 187, 310, 312, 383
FalI AAGNNNNNCTT 1 cut(s) 400
FatI CATG 1 cut(s) 381
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 2 cut(s) 306, 414
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 306, 414
FspBI CTAG 1 cut(s) 243
GluI GCNGC 2 cut(s) 306, 414
HapII CCGG 1 cut(s) 275
Hin1II CATG 1 cut(s) 385
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HinfI GANTC 4 cut(s) 113, 179, 230, 297
HpaII CCGG 1 cut(s) 275
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 3 cut(s) 94, 234, 327
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 256
Hpy8I GTNNAC 3 cut(s) 94, 234, 327
HpyAV CCTTC 2 cut(s) 145, 262
HpyCH4III ACNGT 2 cut(s) 283, 337
HpyCH4V TGCA 3 cut(s) 94, 308, 407
HpyF10VI GCNNNNNNNGC 2 cut(s) 391, 413
HpyF3I CTNAG 3 cut(s) 111, 127, 301
Hsp92II CATG 1 cut(s) 385
LmnI GCTCC 2 cut(s) 4, 12
Lsp1109I GCAGC 2 cut(s) 292, 400
MaeI CTAG 1 cut(s) 243
MboII GAAGA 2 cut(s) 34, 326
MhlI GDGCHC 2 cut(s) 9, 96
MlyI GAGTC 3 cut(s) 122, 173, 239
MspA1I CMGCKG 3 cut(s) 131, 305, 377
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 2 cut(s) 141, 276
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 391, 413
NciI CCSGG 1 cut(s) 276
NdeI CATATG 1 cut(s) 310
NlaIII CATG 1 cut(s) 385
NspI RCATGY 1 cut(s) 385
PfeI GAWTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 307, 415
PleI GAGTC 3 cut(s) 121, 173, 238
PpsI GAGTC 3 cut(s) 121, 173, 238
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 249
PspPPI RGGWCCY 1 cut(s) 249
PvuII CAGCTG 3 cut(s) 131, 305, 377
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SacI GAGCTC 1 cut(s) 9
SatI GCNGC 2 cut(s) 306, 414
Sau96I GGNCC 1 cut(s) 249
SchI GAGTC 3 cut(s) 122, 173, 239
ScrFI CCNGG 2 cut(s) 141, 276
SduI GDGCHC 2 cut(s) 9, 96
SinI GGWCC 1 cut(s) 249
SspMI CTAG 1 cut(s) 243
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 139, 274
StyI CCWWGG 1 cut(s) 341
TaaI ACNGT 2 cut(s) 283, 337
TaqI TCGA 2 cut(s) 285, 291
TfiI GAWTC 1 cut(s) 297
TscAI CASTG 2 cut(s) 101, 342
TseI GCWGC 2 cut(s) 305, 413
TspDTI ATGAA 1 cut(s) 327
TspRI CASTG 2 cut(s) 101, 342
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 249
XagI CCTNNNNNAGG 1 cut(s) 256
XceI RCATGY 1 cut(s) 385
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.