MD10G1319400.v1.1

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014398
Physical Location & Seq
Reverse (-)
40169759 .. 40170934
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1319400.v1.1.491

Sequence Viewer

Length: 411 bp
ATGGAGATCCCTGCCATCCATCCTTACCAATCTGTCTTCCCTTCTCATCTCATCTATCCATACCATTTGGTCCCAGAAAACTATGTGCACTGGACTGAGACCCCTGAATCTCACATTTTCTCGGCTGACATCCCTGGTGTGAGGAAAGAGGACATAAAAGTAGAAGTGGAGGACTCAGTGTACCTCATAATTCGAACAGAGAGGATCGAGGAGGAGTCCACGGAGCAGCCTAGCAGCAGGACCTTCATGAGGAAGTTCCGGCTTCCGGGCCGGGTCGATATCGAACGGATTTCAGCCAGATATGAAGACGGAGTGTTGACAGTCACGGTGCCGAGATCTCTGAGGAGGGCTTTCTTCATTGAGCCAGCTGATGTGCCAGAAAGGCTTGAAGTTCTTGCAAGGGCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

137

Amino Acids

15.88

Weight (kDa)

5.39

Isoelectric Point (pI)

72.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 31 - 114 1.2e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 328
AclWI GGATC 1 cut(s) 212
AfaI GTAC 1 cut(s) 182
AfiI CCNNNNNNNGG 2 cut(s) 249, 265
AgsI TTSAA 1 cut(s) 389
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 1 cut(s) 368
AluI AGCT 1 cut(s) 368
Alw21I GWGCWC 1 cut(s) 90
Alw26I GTCTC 1 cut(s) 92
Alw44I GTGCAC 1 cut(s) 86
AlwI GGATC 1 cut(s) 212
AoxI GGCC 1 cut(s) 268
ApaLI GTGCAC 1 cut(s) 86
ApeKI GCWGC 3 cut(s) 226, 234, 404
AspS9I GGNCC 3 cut(s) 70, 240, 268
AsuC2I CCSGG 2 cut(s) 267, 272
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 2 cut(s) 70, 240
BaeGI GKGCMC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 28, 312
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 3 cut(s) 238, 246, 391
BccI CCATC 2 cut(s) 23, 27
BciT130I CCWGG 1 cut(s) 135
BcnI CCSGG 2 cut(s) 267, 272
BcoDI GTCTC 1 cut(s) 92
BfaI CTAG 1 cut(s) 231
BglI GCCNNNNNGGC 1 cut(s) 382
BglII AGATCT 1 cut(s) 335
BisI GCNGC 3 cut(s) 227, 235, 405
BlsI GCNGC 3 cut(s) 228, 236, 406
Bme1390I CCNGG 3 cut(s) 135, 267, 272
Bme18I GGWCC 2 cut(s) 70, 240
BmgT120I GGNCC 3 cut(s) 70, 240, 268
BmiI GGNNCC 2 cut(s) 72, 330
BmrFI CCNGG 3 cut(s) 135, 267, 272
BpiI GAAGAC 2 cut(s) 28, 312
Bpu14I TTCGAA 1 cut(s) 193
BpuMI CCSGG 2 cut(s) 267, 272
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 2 cut(s) 133, 219
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 265
Bse1I ACTGG 1 cut(s) 95
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 133, 219
BseGI GGATG 3 cut(s) 15, 19, 129
BseLI CCNNNNNNNGG 2 cut(s) 249, 265
BseMII CTCAG 3 cut(s) 87, 189, 332
BseNI ACTGG 1 cut(s) 95
BseRI GAGGAG 3 cut(s) 224, 227, 358
BseSI GKGCMC 1 cut(s) 90
BseXI GCAGC 3 cut(s) 238, 246, 391
BshFI GGCC 1 cut(s) 270
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 3 cut(s) 259, 266, 271
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 249, 265
BsmAI GTCTC 1 cut(s) 92
BsmFI GGGAC 1 cut(s) 56
BsnI GGCC 1 cut(s) 270
Bso31I GGTCTC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 3 cut(s) 6, 204, 335
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 3 cut(s) 88, 188, 333
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 72, 330
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 193
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 1 cut(s) 95
BssECI CCNNGG 2 cut(s) 133, 219
BssMI GATC 3 cut(s) 6, 204, 335
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 2 cut(s) 322, 328
BstAPI GCANNNNNTGC 1 cut(s) 404
BstBI TTCGAA 1 cut(s) 193
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 4 cut(s) 96, 175, 341, 408
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 247
BstF5I GGATG 3 cut(s) 15, 19, 129
BstKTI GATC 3 cut(s) 9, 207, 338
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 3 cut(s) 6, 204, 335
BstMWI GCNNNNNNNGC 2 cut(s) 382, 404
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 3 cut(s) 133, 265, 270
BstSLI GKGCMC 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 238, 246, 391
BstV2I GAAGAC 2 cut(s) 28, 312
BstX2I RGATCY 2 cut(s) 6, 335
BstYI RGATCY 2 cut(s) 6, 335
BsuRI GGCC 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 3 cut(s) 15, 19, 129
BtsIMutI CAGTG 2 cut(s) 88, 183
Cac8I GCNNGC 1 cut(s) 366
CciI TCATGA 1 cut(s) 246
Cfr13I GGNCC 3 cut(s) 70, 240, 268
Csp6I GTAC 1 cut(s) 181
CviAII CATG 1 cut(s) 247
CviQI GTAC 1 cut(s) 181
DdeI CTNAG 4 cut(s) 96, 175, 341, 408
DpnI GATC 3 cut(s) 8, 206, 337
DpnII GATC 3 cut(s) 6, 204, 335
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 2 cut(s) 70, 240
EcoNI CCTNNNNNAGG 1 cut(s) 247
EcoO109I RGGNCCY 1 cut(s) 240
EcoRII CCWGG 1 cut(s) 133
EcoRV GATATC 1 cut(s) 280
FaeI CATG 1 cut(s) 250
FaiI YATR 6 cut(s) 61, 84, 155, 188, 248, 303
FalI AAGNNNNNCTT 1 cut(s) 391
FaqI GGGAC 1 cut(s) 56
FatI CATG 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 227, 235, 405
FokI GGATG 3 cut(s) 2, 6, 116
Fsp4HI GCNGC 3 cut(s) 227, 235, 405
FspBI CTAG 1 cut(s) 231
GluI GCNGC 3 cut(s) 227, 235, 405
HaeIII GGCC 1 cut(s) 270
HapII CCGG 3 cut(s) 259, 266, 271
Hin1II CATG 1 cut(s) 250
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 3 cut(s) 107, 173, 215
HpaII CCGG 3 cut(s) 259, 266, 271
Hpy166II GTNNAC 4 cut(s) 88, 181, 219, 318
Hpy188I TCNGA 1 cut(s) 342
Hpy188III TCNNGA 1 cut(s) 247
Hpy8I GTNNAC 4 cut(s) 88, 181, 219, 318
HpyAV CCTTC 2 cut(s) 51, 253
HpyCH4III ACNGT 2 cut(s) 322, 328
HpyCH4V TGCA 2 cut(s) 88, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 404
HpyF3I CTNAG 4 cut(s) 96, 175, 341, 408
Hsp92II CATG 1 cut(s) 250
Kzo9I GATC 3 cut(s) 6, 204, 335
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 3 cut(s) 238, 246, 391
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 322
MalI GATC 3 cut(s) 8, 206, 337
MboI GATC 3 cut(s) 6, 204, 335
MboII GAAGA 3 cut(s) 28, 317, 346
MflI RGATCY 2 cut(s) 6, 335
MhlI GDGCHC 1 cut(s) 90
MluCI AATT 1 cut(s) 189
MlyI GAGTC 2 cut(s) 167, 224
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 259, 266, 271
MspR9I CCNGG 3 cut(s) 135, 267, 272
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 382, 404
NciI CCSGG 2 cut(s) 267, 272
NdeII GATC 3 cut(s) 6, 204, 335
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 2 cut(s) 72, 330
NmeAIII GCCGAG 2 cut(s) 101, 357
NmuCI GTSAC 1 cut(s) 322
NspV TTCGAA 1 cut(s) 193
PagI TCATGA 1 cut(s) 246
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 3 cut(s) 228, 236, 406
PleI GAGTC 2 cut(s) 167, 223
PpsI GAGTC 2 cut(s) 167, 223
PpuMI RGGWCCY 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 72, 330
PspPI GGNCC 3 cut(s) 70, 240, 268
PspPPI RGGWCCY 1 cut(s) 240
PsuI RGATCY 2 cut(s) 6, 335
PvuII CAGCTG 1 cut(s) 368
RsaI GTAC 1 cut(s) 182
RsaNI GTAC 1 cut(s) 181
SatI GCNGC 3 cut(s) 227, 235, 405
Sau3AI GATC 3 cut(s) 6, 204, 335
Sau96I GGNCC 3 cut(s) 70, 240, 268
SchI GAGTC 2 cut(s) 167, 224
ScrFI CCNGG 3 cut(s) 135, 267, 272
SduI GDGCHC 1 cut(s) 90
SetI ASST 3 cut(s) 186, 245, 370
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 2 cut(s) 70, 240
Sse9I AATT 1 cut(s) 189
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 133, 265, 270
TaaI ACNGT 2 cut(s) 322, 328
TaqI TCGA 4 cut(s) 193, 207, 276, 282
TasI AATT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 2 cut(s) 95, 183
TseFI GTSAC 1 cut(s) 322
TseI GCWGC 3 cut(s) 226, 234, 404
Tsp45I GTSAC 1 cut(s) 322
TspDTI ATGAA 3 cut(s) 235, 318, 346
TspGWI ACGGA 3 cut(s) 236, 301, 324
TspRI CASTG 2 cut(s) 95, 183
VneI GTGCAC 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 70, 240
XagI CCTNNNNNAGG 1 cut(s) 247
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.