Rh5CG033700

15.4 kDa class V heat shock

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0014398
Physical Location & Seq
Forward (+)
2180353 .. 2180868
516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG033700.1

Sequence Viewer

Length: 435 bp
ATGGAGCTCCCAAACCAATACTTCCAGCTTTACCGATATGTCTTCCCCTCTCATCTTGTTTATCCATATCACTTGACCCCAGAAAACTATGTGCACTGGACTGAAACCCCAGAGTCTCACATTTTCTCAGCTGACCTCCCTGGTGTGAGGAAGGAGGAGATAAAAGTGGAAGTGGAGGACTCAATATACCTGATAATCAGAACACAGAGACTTGATGGTGATGGTGATGGTGATGGTGATGAGGAGTCCACAGAGGCTAGGAGGACCTTCACGAGGAAGTTTCGGCTTCCGGGTATGGTCGATATCGAAAGAATCTCAGCTGCATATGAAGATGGTGTGTTGACAATCACAGTGCCAAGGTCATTGAGGAGGAGTTTCTTTATTGAACCAGCTGACATGCCTGAAAGGCTTGATGTTCTTGCAAGAGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009408 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

144

Amino Acids

16.78

Weight (kDa)

4.9

Isoelectric Point (pI)

60.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 33 - 122 8.5e-18 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21870
fragaria_vesca FvH4_3g02330
malus_domestica MD05G1343700.v1.1 MD10G1319400.v1.1
prunus_persica Prupe.4G023100_v2.0.a1
pyrus_communis pycom05g31310 pycom10g27030
rosa_chinensis RchiOBHm_Chr5g0003551
rosa_laevigata RLG00000031151
rosa_multiflora Rmu_sc0004279.1_g000008 Rmu_sc0009787.1_g000003
rosa_roxburghii Rroxscaffold_1G00072410
rosa_rugosa Rorug04G0404100
rosa_samantha Rh5AG031200 Rh5CG033700 Rh5DG030900
rosa_wichuraiana Rw5G002910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 273
AgsI TTSAA 1 cut(s) 386
AjnI CCWGG 1 cut(s) 139
AluBI AGCT 6 cut(s) 7, 28, 131, 320, 392, 428
AluI AGCT 6 cut(s) 7, 28, 131, 320, 392, 428
Alw21I GWGCWC 2 cut(s) 9, 96
Alw26I GTCTC 2 cut(s) 120, 202
Alw44I GTGCAC 1 cut(s) 92
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 320, 428
AspS9I GGNCC 1 cut(s) 264
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 230, 236, 242, 248
AvaII GGWCC 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 9
BauI CACGAG 1 cut(s) 271
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 9, 96
BbvI GCAGC 2 cut(s) 307, 415
BccI CCATC 5 cut(s) 209, 215, 221, 227, 326
BciT130I CCWGG 1 cut(s) 141
BcnI CCSGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 120, 202
BfaI CTAG 1 cut(s) 258
BglI GCCNNNNNGGC 1 cut(s) 406
BisI GCNGC 2 cut(s) 321, 429
BlsI GCNGC 2 cut(s) 322, 430
Bme1390I CCNGG 2 cut(s) 141, 291
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 264
BmrFI CCNGG 2 cut(s) 141, 291
BpiI GAAGAC 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 139, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse1I ACTGG 1 cut(s) 101
BseBI CCWGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 139, 356
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 141, 330
BseNI ACTGG 1 cut(s) 101
BseRI GAGGAG 4 cut(s) 170, 257, 382, 385
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 307, 415
BsiHKAI GWGCWC 2 cut(s) 9, 96
BsiSI CCGG 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 2 cut(s) 120, 202
Bsp1286I GDGCHC 2 cut(s) 9, 96
BspCNI CTCAG 2 cut(s) 140, 329
BsrI ACTGG 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 139, 356
BssSI CACGAG 1 cut(s) 271
BssT1I CCWWGG 1 cut(s) 356
Bst2BI CACGAG 1 cut(s) 271
Bst2UI CCWGG 1 cut(s) 141
Bst4CI ACNGT 1 cut(s) 352
BstAPI GCANNNNNTGC 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 127, 316
BstENI CCTNNNNNAGG 1 cut(s) 271
BstMAI GTCTC 2 cut(s) 120, 202
BstMWI GCNNNNNNNGC 2 cut(s) 406, 428
BstNI CCWGG 1 cut(s) 141
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 139, 289
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 307, 415
BstV2I GAAGAC 1 cut(s) 34
BtsIMutI CAGTG 2 cut(s) 94, 357
Cfr13I GGNCC 1 cut(s) 264
CviAII CATG 1 cut(s) 397
CviJI RGCY 9 cut(s) 7, 28, 131, 257, 286, 320, 392, 409, 428
CviKI_1 RGCY 9 cut(s) 7, 28, 131, 257, 286, 320, 392, 409, 428
DdeI CTNAG 2 cut(s) 127, 316
Ecl136II GAGCTC 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 1 cut(s) 9
Eco32I GATATC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 7
EcoICRI GAGCTC 1 cut(s) 7
EcoNI CCTNNNNNAGG 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 264
EcoRII CCWGG 1 cut(s) 139
EcoRV GATATC 1 cut(s) 304
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 1 cut(s) 400
FaiI YATR 8 cut(s) 39, 67, 90, 187, 296, 325, 327, 398
FalI AAGNNNNNCTT 1 cut(s) 415
FatI CATG 1 cut(s) 396
FauNDI CATATG 1 cut(s) 325
Fnu4HI GCNGC 2 cut(s) 321, 429
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 2 cut(s) 321, 429
FspBI CTAG 1 cut(s) 258
GluI GCNGC 2 cut(s) 321, 429
HapII CCGG 1 cut(s) 290
Hin1II CATG 1 cut(s) 400
HincII GTYRAC 1 cut(s) 342
HindII GTYRAC 1 cut(s) 342
HinfI GANTC 4 cut(s) 113, 179, 245, 312
HpaII CCGG 1 cut(s) 290
HphI GGTGA 4 cut(s) 230, 236, 242, 248
Hpy166II GTNNAC 3 cut(s) 94, 249, 342
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 3 cut(s) 94, 249, 342
HpyAV CCTTC 2 cut(s) 145, 277
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4V TGCA 3 cut(s) 94, 323, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 406, 428
HpyF3I CTNAG 2 cut(s) 127, 316
Hsp92II CATG 1 cut(s) 400
LmnI GCTCC 2 cut(s) 4, 12
Lsp1109I GCAGC 2 cut(s) 307, 415
MaeI CTAG 1 cut(s) 258
MboII GAAGA 2 cut(s) 34, 341
MhlI GDGCHC 2 cut(s) 9, 96
MlyI GAGTC 3 cut(s) 122, 173, 254
MspA1I CMGCKG 3 cut(s) 131, 320, 392
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 141, 291
MvaI CCWGG 1 cut(s) 141
MwoI GCNNNNNNNGC 2 cut(s) 406, 428
NciI CCSGG 1 cut(s) 291
NdeI CATATG 1 cut(s) 325
NlaIII CATG 1 cut(s) 400
NspI RCATGY 1 cut(s) 400
PfeI GAWTC 1 cut(s) 312
PkrI GCNGC 2 cut(s) 322, 430
PleI GAGTC 3 cut(s) 121, 173, 253
PpsI GAGTC 3 cut(s) 121, 173, 253
PpuMI RGGWCCY 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 139
PspGI CCWGG 1 cut(s) 139
PspPI GGNCC 1 cut(s) 264
PspPPI RGGWCCY 1 cut(s) 264
PvuII CAGCTG 3 cut(s) 131, 320, 392
SacI GAGCTC 1 cut(s) 9
SatI GCNGC 2 cut(s) 321, 429
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 3 cut(s) 122, 173, 254
ScrFI CCNGG 2 cut(s) 141, 291
SduI GDGCHC 2 cut(s) 9, 96
SinI GGWCC 1 cut(s) 264
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 139, 289
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 1 cut(s) 352
TaqI TCGA 2 cut(s) 300, 306
TfiI GAWTC 1 cut(s) 312
TscAI CASTG 2 cut(s) 101, 357
TseI GCWGC 2 cut(s) 320, 428
TspDTI ATGAA 1 cut(s) 342
TspRI CASTG 2 cut(s) 101, 357
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 271
XceI RCATGY 1 cut(s) 400
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.