Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003447

Physical Location & Seq

Genomic Coordinates Reverse (-)

11746490 .. 11750698

Gene Structure 4209 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G22200.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGACCTCAAGTATTCAGCATCTCATTGCAACTTATCCTCAGACATGAAGCTCAGGCGTTTTCATCAGCATCGAGGAAAAGGAAGAGAAGAAGAGTATGATGCTTCTTCTCTCAGCTTGAACAATCTGTCAAAACTTATTCTTCCTCCACTTGGTGTTGCTAGCTATAACCAGAATCACATCAGGTCTAGTGGATGGATCATCTCACCTATGGACTCAAGATACAGGTGCTGGGAATTTTATATGGTGCTTTTAGTGGCATACTCTGCGTGGGTTTACCCTTTTGAAGTTGCATTTCTGAATTCATCACCAAAGAGAAACCTTTGTATCGCGGACAACATCGTAGACTTGTTCTTCGCGGTTGACATTGTCTTGACGTTTTTCGTTGCTTACATAGACGAAAGAACACAGCTTCTTGTCCGTGAACCTAAACAGATTGCAGTGAGGTACCTATCAACATGGTTCTTGATGGATGTTGCATCAACTATACCATTTGACGCTATTGGATACTTAATCACTGGCACATCCACGTTAAATATCACTTGTAATCTCTTGGGATTACTTAGATTTTGGCGACTTCGAAGAGTTAAACACCTCTTCACTAGGCTCGAGAAGGACATAAGATATAGCTATTTCTGGATTCGCTGCTTTCGACTTCTATCAGTGACATTGTTTCTAGTGCACTGTGCTGGATGCAGTTATTACCTAATAGCAGACAGATATCCACACCAAGGAAAGACATGGACTGATGCGATCCCTAATTTCACAGAGACAAGTCTTTCCATCAGATACATTGCAGCTATATATTGGTCTATCACTACAATGACCACAGTGGGATATGGAGATCTTCATGCAAGCAACACTATTGAAATGGTATTCATAACAGTCTACATGTTATTCAATCTTGGCCTCACTGCTTACCTTATTGGTAACATGACTAATTTGGTCGTGGAAGGGACTCGTCGTACCATGGAATTTAGGAATAGCATTGAAGCAGCGTCAAACTTTGTTAACAGAAACAGATTGCCTCCTAGATTAAAAGACCAGATATTAGCTTACATGTGTTTAAGGTTTAAAGCAGAGAGCTTAAATCAGCAACATCTTATTGACCAGCTCCCAAAATCTATCTACAAAAGCATTTGTCAACATCTTTTTCTTCCATCTGTTGAAAAAGTTTACCTCTTCAAAGGCGTCTCAAGAGAAATACTTCTTCTTCTGGTTTCAAAAATGAAGGCTGAGTATATACCACCAAGAGAGGATGTCATTATGCAGAACGAAGCGCCGGATGATGTTTACATAATTGTGTCAGGAGAAGTTGAGATCATTGATTCAGAGATGGAGAGAGAGTCTGTTTTAGGCACTCTACGTTGTGGAGACATATTTGGAGAAGTTGGAGCACTTTGTTGCAGACCACAAAGCTACACTTTTCAAACTAAGTCTTTATCACAGCTTCTCCGACTCAAAACATCTTTCCTTATTGAGACAATGCAGATTAAACAACAAGACAATGCCACAATGCTCAAGAACTTCTTGCAGCATCACAAAAAGCTGAGTAATTTAGACATTGGTGATCTAAAGGCACAACAAAATGGCGAAAACACCGATGTTGTTCCTCCTAACATTGCCTCAAATCTCATCGCTGTGGTGACTACAGGCAATGCAGCTCTTCTTGATGAGCTACTTAAGGCTAAGTTAAGCCCTGACATTACAGATTCCAAAGGAAAAACTCCATTGCATGTAGCAGCTTCTAGAGGATATGAAGATTGTGTTTTAGTACTCTTAAAGCACGGTTGCAACATCCACATAAGAGATGTGAATGGTAATAGTGCTCTATGGGAAGCAATAATATCGAAGCATTACGAGATATTCAGAATCCTTTATCATTTCGCAGCCATATCGGATCCACACATAGCTGGAGATCTTCTATGTGAAGCAGCGAAACAGAACAATGTAGAAGTCATGAAGGCTCTTTTAAAACAGGGGCTTAACGTCGACACAGAGGATCACCATGGCGTCACAGCTTTACAGGTCGCTATGGCGGAGGATCAGATGGACATGGTGAATCTCCTGGCGACGAACGGTGCAGATGTAGTTTGTGTGAATACACATAATGAATTCACACCATTGGAGAAGTTAAGAGTTGTGGAAGAAGAAGAAGAAGAAGAACGAGGAAGAGTGAGTATTTACAGAGGACATCCATTGGAGAGGAGAGAAAGAAGTTGCAATGAAGCTGGGAAGCTTATTCTTCTTCCTCCTTCACTTGATGACCTCAAGAAAATTGCAGGAGAGAAGTTTGGGTTTGATGGAAGTGAGACGATGGTGACGAATGAAGATGGAGCTGAGATTGACAGTATTGAAGTGATTAGAGATAATGACAAACTCTACTTTGTCGTAAACAAGATAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0031984 GO:0033993 GO:0034220 GO:0042175 GO:0042221 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

802

Amino Acids

91.31

Weight (kDa)

6.09

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	75 - 323	6.8e-45	Ion transport protein
Ion_trans_2	PF07885	225 - 317	1.1e-12	Ion channel
cNMP_binding	PF00027	414 - 497	1.1e-14	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22200 AT4G22200
fragaria_vesca	FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980
malus_domestica	MD08G1236000.v1.1
prunus_persica	Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1
pyrus_communis	pycom08g20420
rosa_chinensis	RchiOBHm_Chr7g0221301 RchiOBHm_Chr7g0222541
rosa_laevigata	RLG00000002163
rosa_multiflora	Rmu_co8307231.1_g000001 Rmu_sc0000324.1_g000021
rosa_roxburghii	Rroxscaffold_3G00238430
rosa_rugosa	Rorug07G0202000
rosa_samantha	Rh6BG194600 Rh7AG341200 Rh7AG352200 Rh7BG332200 Rh7CG359100 Rh7CG369500 Rh7DG339700
rosa_wichuraiana	Rw7G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	447
AccB1I	GGYRCC	1 cut(s)	447
AccI	GTMKAC	3 cut(s)	345, 888, 1992
AccII	CGCG	2 cut(s)	332, 359
AciI	CCGC	3 cut(s)	332, 359, 2039
AclWI	GGATC	6 cut(s)	206, 748, 1895, 1908, 2010, 2052
AcsI	RAATTY	4 cut(s)	236, 301, 974, 2114
AcyI	GRCGYC	2 cut(s)	1191, 2013
AdeI	CACNNNGTG	1 cut(s)	155
AfaI	GTAC	3 cut(s)	449, 967, 1776
AfiI	CCNNNNNNNGG	2 cut(s)	152, 731
AflII	CTTAAG	1 cut(s)	1682
AflIII	ACRYGT	2 cut(s)	891, 1059
AjnI	CCWGG	1 cut(s)	2067
Alw21I	GWGCWC	3 cut(s)	684, 1399, 1831
Alw26I	GTCTC	5 cut(s)	764, 1198, 1368, 1475, 2306
Alw44I	GTGCAC	1 cut(s)	680
AlwI	GGATC	6 cut(s)	206, 748, 1895, 1908, 2010, 2052
AlwNI	CAGNNNCTG	1 cut(s)	231
Ama87I	CYCGRG	1 cut(s)	608
AoxI	GGCC	1 cut(s)	907
ApaLI	GTGCAC	1 cut(s)	680
ApeKI	GCWGC	8 cut(s)	645, 797, 995, 1534, 1661, 1742, 1889, 1934
ApoI	RAATTY	4 cut(s)	236, 301, 974, 2114
ArsI	GACNNNNNNTTYG	2 cut(s)	338, 370
Asp700I	GAANNNNTTC	1 cut(s)	1206
Asp718I	GGTACC	1 cut(s)	447
AspLEI	GCGC	1 cut(s)	1282
AspS9I	GGNCC	1 cut(s)	4
AsuHPI	GGTGA	7 cut(s)	198, 300, 1580, 1657, 1997, 2071, 2332
AsuII	TTCGAA	1 cut(s)	580
AsuNHI	GCTAGC	1 cut(s)	161
AvaI	CYCGRG	1 cut(s)	608
AvaII	GGWCC	1 cut(s)	4
BaeGI	GKGCMC	1 cut(s)	684
BaeI	ACNNNNGTAYC	2 cut(s)	310, 343
BamHI	GGATCC	1 cut(s)	1900
BanI	GGYRCC	1 cut(s)	447
Bbv12I	GWGCWC	3 cut(s)	684, 1399, 1831
BbvI	GCAGC	8 cut(s)	632, 809, 1007, 1546, 1673, 1754, 1901, 1946
BccI	CCATC	9 cut(s)	189, 463, 791, 1168, 1330, 2044, 2297, 2311, 2327
BcgI	CGANNNNNNTGC	4 cut(s)	1830, 1864, 1878, 1912
BciT130I	CCWGG	1 cut(s)	2069
BciVI	GTATCC	1 cut(s)	500
BcoDI	GTCTC	5 cut(s)	764, 1198, 1368, 1475, 2306
BfaI	CTAG	6 cut(s)	162, 189, 603, 677, 1032, 1749
BfmI	CTRYAG	1 cut(s)	1650
BfoI	RGCGCY	1 cut(s)	1283
BfrI	CTTAAG	1 cut(s)	1682
BfuI	GTATCC	1 cut(s)	500
BglII	AGATCT	2 cut(s)	844, 1918
BisI	GCNGC	8 cut(s)	646, 798, 996, 1535, 1662, 1743, 1890, 1935
BlsI	GCNGC	8 cut(s)	647, 799, 997, 1536, 1663, 1744, 1891, 1936
BmcAI	AGTACT	1 cut(s)	1776
Bme1390I	CCNGG	1 cut(s)	2069
Bme18I	GGWCC	1 cut(s)	4
BmeT110I	CYCGRG	1 cut(s)	608
BmgT120I	GGNCC	1 cut(s)	4
BmiI	GGNNCC	2 cut(s)	449, 1902
BmrFI	CCNGG	1 cut(s)	2069
BmsI	GCATC	7 cut(s)	29, 79, 91, 488, 683, 739, 1546
BmtI	GCTAGC	1 cut(s)	165
BpmI	CTGGAG	1 cut(s)	1935
Bpu10I	CCTNAGC	1 cut(s)	53
Bpu14I	TTCGAA	1 cut(s)	580
BpuEI	CTTGAG	4 cut(s)	202, 1180, 1505, 2255
BsaHI	GRCGYC	2 cut(s)	1191, 2013
BsaJI	CCNNGG	3 cut(s)	730, 969, 2008
BsaXI	ACNNNNNCTCC	4 cut(s)	1331, 1361, 1437, 1467
Bsc4I	CCNNNNNNNGG	2 cut(s)	152, 731
Bse1I	ACTGG	1 cut(s)	523
Bse3DI	GCAATG	6 cut(s)	25, 792, 1620, 1663, 1730, 2230
BseBI	CCWGG	1 cut(s)	2069
BseDI	CCNNGG	3 cut(s)	730, 969, 2008
BseGI	GGATG	8 cut(s)	200, 478, 524, 698, 1264, 1291, 1797, 2194
BseLI	CCNNNNNNNGG	2 cut(s)	152, 731
BseMI	GCAATG	6 cut(s)	25, 792, 1620, 1663, 1730, 2230
BseMII	CTCAG	6 cut(s)	54, 67, 127, 1227, 1541, 2331
BseNI	ACTGG	1 cut(s)	523
BseRI	GAGGAG	1 cut(s)	2221
BseSI	GKGCMC	1 cut(s)	684
BseXI	GCAGC	8 cut(s)	632, 809, 1007, 1546, 1673, 1754, 1901, 1946
BseYI	CCCAGC	2 cut(s)	231, 2231
BsgI	GTGCAG	1 cut(s)	2103
Bsh1236I	CGCG	2 cut(s)	332, 359
BshFI	GGCC	1 cut(s)	909
BshNI	GGYRCC	1 cut(s)	447
BsiHKAI	GWGCWC	3 cut(s)	684, 1399, 1831
BsiHKCI	CYCGRG	1 cut(s)	608
BsiSI	CCGG	1 cut(s)	1283
BslFI	GGGAC	1 cut(s)	970
BslI	CCNNNNNNNGG	2 cut(s)	152, 731
BsmAI	GTCTC	5 cut(s)	764, 1198, 1368, 1475, 2306
BsmBI	CGTCTC	2 cut(s)	1198, 2306
BsmFI	GGGAC	1 cut(s)	970
BsnI	GGCC	1 cut(s)	909
BsoBI	CYCGRG	1 cut(s)	608
Bsp119I	TTCGAA	1 cut(s)	580
Bsp1286I	GDGCHC	3 cut(s)	684, 1399, 1831
Bsp143I	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
Bsp19I	CCATGG	2 cut(s)	969, 2008
BspACI	CCGC	3 cut(s)	332, 359, 2039
BspANI	GGCC	1 cut(s)	909
BspCNI	CTCAG	6 cut(s)	53, 66, 126, 1228, 1542, 2332
BspFNI	CGCG	2 cut(s)	332, 359
BspHI	TCATGA	1 cut(s)	1959
BspLI	GGNNCC	2 cut(s)	449, 1902
BspOI	GCTAGC	1 cut(s)	165
BspPI	GGATC	6 cut(s)	206, 748, 1895, 1908, 2010, 2052
BspQI	GCTCTTC	1 cut(s)	1671
BspT104I	TTCGAA	1 cut(s)	580
BspT107I	GGYRCC	1 cut(s)	447
BspTI	CTTAAG	1 cut(s)	1682
BsrDI	GCAATG	6 cut(s)	25, 792, 1620, 1663, 1730, 2230
BsrI	ACTGG	1 cut(s)	523
BssECI	CCNNGG	3 cut(s)	730, 969, 2008
BssMI	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
BssNI	GRCGYC	2 cut(s)	1191, 2013
BssT1I	CCWWGG	3 cut(s)	730, 969, 2008
Bst2UI	CCWGG	1 cut(s)	2069
Bst4CI	ACNGT	6 cut(s)	686, 832, 886, 1790, 2081, 2351
Bst6I	CTCTTC	7 cut(s)	79, 87, 577, 602, 1187, 1671, 2167
BstACI	GRCGYC	2 cut(s)	1191, 2013
BstAFI	CTTAAG	1 cut(s)	1682
BstAPI	GCANNNNNTGC	1 cut(s)	266
BstBI	TTCGAA	1 cut(s)	580
BstC8I	GCNNGC	2 cut(s)	163, 856
BstDEI	CTNAG	9 cut(s)	40, 53, 113, 563, 1236, 1434, 1550, 1689, 2340
BstDSI	CCRYGG	2 cut(s)	969, 2008
BstF5I	GGATG	8 cut(s)	200, 478, 524, 698, 1264, 1291, 1797, 2194
BstFNI	CGCG	2 cut(s)	332, 359
BstH2I	RGCGCY	1 cut(s)	1283
BstHHI	GCGC	1 cut(s)	1282
BstKTI	GATC	9 cut(s)	201, 756, 847, 1323, 1573, 1903, 1921, 2005, 2047
BstMAI	GTCTC	5 cut(s)	764, 1198, 1368, 1475, 2306
BstMBI	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
BstMWI	GCNNNNNNNGC	1 cut(s)	266
BstNI	CCWGG	1 cut(s)	2069
BstNSI	RCATGY	3 cut(s)	895, 1063, 1739
BstSCI	CCNGG	1 cut(s)	2067
BstSFI	CTRYAG	1 cut(s)	1650
BstSLI	GKGCMC	1 cut(s)	684
BstUI	CGCG	2 cut(s)	332, 359
BstV1I	GCAGC	8 cut(s)	632, 809, 1007, 1546, 1673, 1754, 1901, 1946
BstX2I	RGATCY	3 cut(s)	844, 1900, 1918
BstYI	RGATCY	3 cut(s)	844, 1900, 1918
BsuI	GTATCC	1 cut(s)	500
BsuRI	GGCC	1 cut(s)	909
BtgI	CCRYGG	2 cut(s)	969, 2008
BtgZI	GCGATG	1 cut(s)	1621
BtsCI	GGATG	8 cut(s)	200, 478, 524, 698, 1264, 1291, 1797, 2194
BtsI	GCAGTG	2 cut(s)	447, 912
BtsIMutI	CAGTG	6 cut(s)	447, 516, 669, 682, 837, 912
Cac8I	GCNNGC	2 cut(s)	163, 856
CaiI	CAGNNNCTG	1 cut(s)	231
CciI	TCATGA	1 cut(s)	1959
CfoI	GCGC	1 cut(s)	1282
Cfr13I	GGNCC	1 cut(s)	4
CseI	GACGC	4 cut(s)	506, 987, 1180, 2002
Csp6I	GTAC	3 cut(s)	448, 966, 1775
CviQI	GTAC	3 cut(s)	448, 966, 1775
DdeI	CTNAG	9 cut(s)	40, 53, 113, 563, 1236, 1434, 1550, 1689, 2340
DpnI	GATC	9 cut(s)	200, 755, 846, 1322, 1572, 1902, 1920, 2004, 2046
DpnII	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
DraI	TTTAAA	2 cut(s)	1075, 1974
DraIII	CACNNNGTG	1 cut(s)	155
Eam1104I	CTCTTC	7 cut(s)	79, 87, 577, 602, 1187, 1671, 2167
EarI	CTCTTC	7 cut(s)	79, 87, 577, 602, 1187, 1671, 2167
EciI	GGCGGA	1 cut(s)	2054
Eco130I	CCWWGG	3 cut(s)	730, 969, 2008
Eco32I	GATATC	1 cut(s)	722
Eco47I	GGWCC	1 cut(s)	4
Eco88I	CYCGRG	1 cut(s)	608
EcoRI	GAATTC	2 cut(s)	301, 2114
EcoRII	CCWGG	1 cut(s)	2067
EcoRV	GATATC	1 cut(s)	722
EcoT14I	CCWWGG	3 cut(s)	730, 969, 2008
ErhI	CCWWGG	3 cut(s)	730, 969, 2008
Esp3I	CGTCTC	2 cut(s)	1198, 2306
FalI	AAGNNNNNCTT	4 cut(s)	1408, 1440, 1514, 1546
FaqI	GGGAC	1 cut(s)	970
FblI	GTMKAC	3 cut(s)	345, 888, 1992
Fnu4HI	GCNGC	8 cut(s)	646, 798, 996, 1535, 1662, 1743, 1890, 1935
FokI	GGATG	8 cut(s)	207, 485, 511, 705, 1271, 1298, 1784, 2181
Fsp4HI	GCNGC	8 cut(s)	646, 798, 996, 1535, 1662, 1743, 1890, 1935
FspBI	CTAG	6 cut(s)	162, 189, 603, 677, 1032, 1749
GlaI	GCGC	1 cut(s)	1281
GluI	GCNGC	8 cut(s)	646, 798, 996, 1535, 1662, 1743, 1890, 1935
GsaI	CCCAGC	2 cut(s)	235, 2235
GsuI	CTGGAG	1 cut(s)	1935
HaeII	RGCGCY	1 cut(s)	1283
HaeIII	GGCC	1 cut(s)	909
HapII	CCGG	1 cut(s)	1283
HgaI	GACGC	4 cut(s)	506, 987, 1180, 2002
HhaI	GCGC	1 cut(s)	1282
Hin1I	GRCGYC	2 cut(s)	1191, 2013
Hin6I	GCGC	1 cut(s)	1280
HinP1I	GCGC	1 cut(s)	1280
HincII	GTYRAC	4 cut(s)	364, 1012, 1145, 1993
HindII	GTYRAC	4 cut(s)	364, 1012, 1145, 1993
HindIII	AAGCTT	1 cut(s)	2237
HpaI	GTTAAC	1 cut(s)	1012
HpaII	CCGG	1 cut(s)	1283
HphI	GGTGA	7 cut(s)	198, 300, 1580, 1657, 1997, 2071, 2332
Hpy188I	TCNGA	8 cut(s)	43, 300, 788, 1333, 1457, 1871, 1900, 2049
Hpy99I	CGWCG	3 cut(s)	966, 1994, 2077
HpyAV	CCTTC	5 cut(s)	607, 947, 1225, 1957, 2265
HpyCH4III	ACNGT	6 cut(s)	686, 832, 886, 1790, 2081, 2351
HpyCH4IV	ACGT	4 cut(s)	377, 530, 1366, 1989
HpyF10VI	GCNNNNNNNGC	1 cut(s)	266
HpyF3I	CTNAG	9 cut(s)	40, 53, 113, 563, 1236, 1434, 1550, 1689, 2340
HpySE526I	ACGT	4 cut(s)	377, 530, 1366, 1989
Hsp92I	GRCGYC	2 cut(s)	1191, 2013
HspAI	GCGC	1 cut(s)	1280
KpnI	GGTACC	1 cut(s)	451
KspAI	GTTAAC	1 cut(s)	1012
Kzo9I	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
LguI	GCTCTTC	1 cut(s)	1671
LmnI	GCTCC	3 cut(s)	1119, 1394, 2336
Lsp1109I	GCAGC	8 cut(s)	632, 809, 1007, 1546, 1673, 1754, 1901, 1946
LweI	GCATC	7 cut(s)	29, 79, 91, 488, 683, 739, 1546
MaeI	CTAG	6 cut(s)	162, 189, 603, 677, 1032, 1749
MaeII	ACGT	4 cut(s)	377, 530, 1366, 1989
MaeIII	GTNAC	5 cut(s)	664, 929, 1645, 2014, 2320
MalI	GATC	9 cut(s)	200, 755, 846, 1322, 1572, 1902, 1920, 2004, 2046
MboI	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
MflI	RGATCY	3 cut(s)	844, 1900, 1918
MhlI	GDGCHC	3 cut(s)	684, 1399, 1831
MlyI	GAGTC	4 cut(s)	209, 952, 1355, 1452
MmeI	TCCRAC	2 cut(s)	1372, 1480
MroXI	GAANNNNTTC	1 cut(s)	1206
MslI	CAYNNNNRTG	3 cut(s)	821, 1301, 1640
MspCI	CTTAAG	1 cut(s)	1682
MspI	CCGG	1 cut(s)	1283
MspR9I	CCNGG	1 cut(s)	2069
MvaI	CCWGG	1 cut(s)	2069
MvnI	CGCG	2 cut(s)	332, 359
MwoI	GCNNNNNNNGC	1 cut(s)	266
NcoI	CCATGG	2 cut(s)	969, 2008
NdeII	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
NheI	GCTAGC	1 cut(s)	161
NlaIV	GGNNCC	2 cut(s)	449, 1902
NmuCI	GTSAC	4 cut(s)	664, 1645, 2014, 2320
NspI	RCATGY	3 cut(s)	895, 1063, 1739
NspV	TTCGAA	1 cut(s)	580
PaeR7I	CTCGAG	1 cut(s)	608
PagI	TCATGA	1 cut(s)	1959
PciI	ACATGT	2 cut(s)	891, 1059
PciSI	GCTCTTC	1 cut(s)	1671
PcsI	WCGNNNNNNNCGW	2 cut(s)	649, 2321
PdmI	GAANNNNTTC	1 cut(s)	1206
PfeI	GAWTC	6 cut(s)	175, 640, 1328, 1712, 1872, 2062
PflFI	GACNNNGTC	1 cut(s)	368
PkrI	GCNGC	8 cut(s)	647, 799, 997, 1536, 1663, 1744, 1891, 1936
PleI	GAGTC	4 cut(s)	209, 952, 1354, 1452
PpsI	GAGTC	4 cut(s)	209, 952, 1354, 1452
PscI	ACATGT	2 cut(s)	891, 1059
Psp6I	CCWGG	1 cut(s)	2067
PspFI	CCCAGC	2 cut(s)	231, 2231
PspGI	CCWGG	1 cut(s)	2067
PspN4I	GGNNCC	2 cut(s)	449, 1902
PspPI	GGNCC	1 cut(s)	4
PstNI	CAGNNNCTG	1 cut(s)	231
PsuI	RGATCY	3 cut(s)	844, 1900, 1918
PsyI	GACNNNGTC	1 cut(s)	368
RsaI	GTAC	3 cut(s)	449, 967, 1776
RsaNI	GTAC	3 cut(s)	448, 966, 1775
RseI	CAYNNNNRTG	3 cut(s)	821, 1301, 1640
SalI	GTCGAC	1 cut(s)	1991
SapI	GCTCTTC	1 cut(s)	1671
SatI	GCNGC	8 cut(s)	646, 798, 996, 1535, 1662, 1743, 1890, 1935
Sau3AI	GATC	9 cut(s)	198, 753, 844, 1320, 1570, 1900, 1918, 2002, 2044
Sau96I	GGNCC	1 cut(s)	4
ScaI	AGTACT	1 cut(s)	1776
SchI	GAGTC	4 cut(s)	209, 952, 1355, 1452
ScrFI	CCNGG	1 cut(s)	2069
SduI	GDGCHC	3 cut(s)	684, 1399, 1831
SfaNI	GCATC	7 cut(s)	29, 79, 91, 488, 683, 739, 1546
SfcI	CTRYAG	1 cut(s)	1650
Sfr274I	CTCGAG	1 cut(s)	608
SfuI	TTCGAA	1 cut(s)	580
SinI	GGWCC	1 cut(s)	4
SlaI	CTCGAG	1 cut(s)	608
SmiMI	CAYNNNNRTG	3 cut(s)	821, 1301, 1640
SmlI	CTYRAG	7 cut(s)	8, 217, 608, 1195, 1520, 1682, 2270
SmoI	CTYRAG	7 cut(s)	8, 217, 608, 1195, 1520, 1682, 2270
SsiI	CCGC	3 cut(s)	332, 359, 2039
SspMI	CTAG	6 cut(s)	162, 189, 603, 677, 1032, 1749
StyD4I	CCNGG	1 cut(s)	2067
StyI	CCWWGG	3 cut(s)	730, 969, 2008
TaaI	ACNGT	6 cut(s)	686, 832, 886, 1790, 2081, 2351
TaiI	ACGT	4 cut(s)	380, 533, 1369, 1992
TaqI	TCGA	6 cut(s)	73, 580, 609, 652, 1850, 1992
TatI	WGTACW	1 cut(s)	1774
TfiI	GAWTC	6 cut(s)	175, 640, 1328, 1712, 1872, 2062
TscAI	CASTG	6 cut(s)	447, 523, 669, 689, 837, 919
TseFI	GTSAC	4 cut(s)	664, 1645, 2014, 2320
TseI	GCWGC	8 cut(s)	645, 797, 995, 1534, 1661, 1742, 1889, 1934
Tsp45I	GTSAC	4 cut(s)	664, 1645, 2014, 2320
TspGWI	ACGGA	1 cut(s)	410
TspRI	CASTG	6 cut(s)	447, 523, 669, 689, 837, 919
Tth111I	GACNNNGTC	1 cut(s)	368
Vha464I	CTTAAG	1 cut(s)	1682
VneI	GTGCAC	1 cut(s)	680
VpaK11BI	GGWCC	1 cut(s)	4
XapI	RAATTY	4 cut(s)	236, 301, 974, 2114
XbaI	TCTAGA	1 cut(s)	1748
XceI	RCATGY	3 cut(s)	895, 1063, 1739
XhoI	CTCGAG	1 cut(s)	608
XmiI	GTMKAC	3 cut(s)	345, 888, 1992
XmnI	GAANNNNTTC	1 cut(s)	1206
XspI	CTAG	6 cut(s)	162, 189, 603, 677, 1032, 1749
ZrmI	AGTACT	1 cut(s)	1776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.