Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003447

Physical Location & Seq

Genomic Coordinates Forward (+)

26583664 .. 26589785

Gene Structure 6122 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002163

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2580 bp

ATGCAGAACAAGGTTTGTGGCTGGAAATTAGGCCAAAATGGGCAGCAGGTGATCAAAGGTCGTCATGAAGCATCAGATGGTGATCAATCACCTCATGATCAACATCACAATTACAAGAATAATGATCGAATGATGTACTCAAACATATCATCGTCGTCGGATCTCAACTTGAGAAACCTCTCAAAGCTTATACTTCCTCCTTTGGGTGCCTCGAGCTACAACCAGCCGACTCAAATGCACTCAAAAGGCTGGATTATTTTACCCATGGACTCCAGATACAGGAGTTGGGAGACATTTATGGTGCTTTTGGTGGCTTACTCAGCCTGGGTTTACCCGTTTGAGGTTGCTTTTCTCATGTCGTCGTCGGGAAAAAGCAGCAAGCCTCTCTACTTAGCAGACTACATTGTTGATCTATTTTTCGGCATCGATATCATATTGACATTCTTCGTCGCATATATTGATCCCAGAACTCAATTACTTGTTCTCGACTCCAAAAAGATCGCTACAAGATATCTTTCGACATGGTTTGTTATGGATTTGGCATCAACCCTCCCCTTTGAGATTATAGGCAAACACAAACTCGGTCTCTCCTACTCTCTCTTGGGATTGCTCCGATTTTGGCGATTGAGACGAGCAAAGCAACTCTTCACAAGGCTTGAGAAGGACATCAGGTTTAGCTACTTCTGGGTCAGATGCGCCAGGCTTTTATCTGTGACACTGTTTTCAGTACATTGTGCTGGGTGCCTGTACTACCTGCTAGCAGACCGATACCCGCACCAAGGGAGGACATGGATTGGAGCTGTTATTCCCAATTTCAGAGAGACGAGTCTGTGGATCCGCTACATTTCAGCCGTCTACTGGTCCATCACCACCATGTCCACCGTTGGTTATGGTGACCTCCATGCAGTGAACACCATGGAAATGATCTTCATCATTTTATACATGTTTTTCAACCTCGGCCTTACTGCTTACCTAATTGGCAACATGACAAACTTAGTTGTTGAAGGCACTCGCCGTACCATGGAATTTAGAAACAGCGTTGAAGCAGCATCAAACTTTGTTGACCGCAATCACTTGCCTCTAAGGTTGAAGAATCAAATTCTGGCCTATATTTGCTTGAGATTTAAGGCTGAGAGCTTGAACCAGCAGCAAATAATTGAACAGCTCCCCAAGTCAATTTGTAAAAGCATCTGTCAACATTTGTTCCTGCCAACAGTAGAGAAAGTTTATCTTTTCAAGGGTGTCTCAAGCGAAATACTTCTAATTCTGGTTGCAAACATGAAGGCAGAGTACATACCACCAAGAGAAGATGTTGTCATGCAAAACGAAGCACCGGATGATATATACATCATTGTTTCTGGAGAAGTGGAAATCATTGATTGTGAGATGGATAAAGAGCTAGTCGTCGGGACTTTGAAGTCTGGGGACATGTTTGGAGAAGTAGGTGCACTTTGTTGCCGAGAAGTTGGTGCACTTTGTTGTAGGCCTCAAAGCTTTACATATCGGACAAAAACACTTTCACAACTCTTGAGACTCAAAACCACTGCACTAATAGAAGCAATGCAGATCAAACAAGAAGACAATGCTCAAATGCTTAAGAACTTCAAGCAGCATCACAAAAAGCTCAGGGATCTGAGAATTGGAGAAATATTGGCTGAGAGTGGGGAAGATGAGGCCATGGGTGATCCAAGTTTAGCATTAGACTTATTGACTATTGCCAGCAAAGGGAATGCTGCACTTCTTGAAGAGCTCCTCAAGTCAAAGTTGGATCCTGACATTGGAGACTCCAAAGGAAGAACACCTCTGCATGTAGCAGCATCAAACGGGCATGAAGAATGTGTCTTGGTACTCCTCAAGTATGCTTGTAACATACATGCAAGAGACACTAATGGTAACACGGCAATATGGGAAGCAATTGCAGCAAAGCACCATTCCATATTCAAGATCCTCTACTATTGCGCTGCCTTTTCTTATCCGTACACTGCCGGTGATCTTCTATGCACAGCTGCCAGAAGAAACGATCTGACTGTGATGAAGGAACTGTTGAAGCATGAATTGCATGTTGACTCAAAGGATTTCGACGGAAAAACAGCAGTACAAGTAGCCATGGAAGAAAACAATGTAGACATGGTAAACTTGCTGGTGATGAATGGAGCAGATGTCACCAATGTTTCACATGACTACTTATTTCCTTCACAAACCTTAAGCGATATGCTGCAAAAACGAGAGGTTGGACACCGGATTACAGTGCCAGACCCTACACTGAATGAAGTGATTCTGTTCAGAAATGATGGAGAGAAAAATGTTGATTGGAGAAAATCCAATGGAATGAATTCTCCAAGAGTGAGCATATATAGAGGCCATCCTGTGATGAGGAGAAACACCTGTTGCACTGAAGCCGGGAGGTTGATTAGATTGCCTTATTCACTAGAGGAGCTCAAAAATATTGCAGGTGAAAAGTTTGGGTTCGATCCAGGGAATGTAATATTGACGGATGAGGAAGGAGCAGAAATAGACTGTATCGAAGTGATTAGAGATAATGACAAGCTTTTCATATATATAGTGGAGGACACAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0031984 GO:0033993 GO:0034220 GO:0042175 GO:0042221 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

860

Amino Acids

97.74

Weight (kDa)

6.56

Isoelectric Point (pI)

32.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	93 - 340	8.3e-42	Ion transport protein
Ion_trans_2	PF07885	265 - 334	4.6e-13	Ion channel
cNMP_binding	PF00027	431 - 522	9.7e-11	Cyclic nucleotide-binding domain
Ank_4	PF13637	571 - 618	1.1e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	572 - 652	9e-15	Ankyrin repeats (3 copies)
Ank_5	PF13857	589 - 635	1.5e-08	Ankyrin repeats (many copies)
Ank	PF00023	598 - 628	4.5e-08	Ankyrin repeat
Ank_2	PF12796	665 - 722	4.5e-08	Ankyrin repeats (3 copies)
KHA	PF11834	781 - 852	1.1e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22200 AT4G22200
fragaria_vesca	FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980
malus_domestica	MD08G1236000.v1.1
prunus_persica	Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1
pyrus_communis	pycom08g20420
rosa_chinensis	RchiOBHm_Chr7g0221301 RchiOBHm_Chr7g0222541
rosa_laevigata	RLG00000002163
rosa_multiflora	Rmu_co8307231.1_g000001 Rmu_sc0000324.1_g000021
rosa_roxburghii	Rroxscaffold_3G00238430
rosa_rugosa	Rorug07G0202000
rosa_samantha	Rh6BG194600 Rh7AG341200 Rh7AG352200 Rh7BG332200 Rh7CG359100 Rh7CG369500 Rh7DG339700
rosa_wichuraiana	Rw7G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	37, 2441
AasI	GACNNNNNNGTC	1 cut(s)	1417
Acc36I	ACCTGC	3 cut(s)	37, 762, 2441
AccB1I	GGYRCC	2 cut(s)	206, 741
AccI	GTMKAC	2 cut(s)	855, 2124
AciI	CCGC	3 cut(s)	773, 838, 1066
AcsI	RAATTY	3 cut(s)	1025, 1098, 2332
AcuI	CTGAAG	1 cut(s)	2415
AfaI	GTAC	8 cut(s)	137, 729, 749, 1018, 1292, 1848, 1979, 2097
AfiI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1725, 1778
AflII	CTTAAG	2 cut(s)	1595, 2203
AflIII	ACRYGT	2 cut(s)	942, 1428
AjnI	CCWGG	3 cut(s)	323, 698, 2473
Alw21I	GWGCWC	4 cut(s)	1450, 1474, 1752, 2439
Alw26I	GTCTC	8 cut(s)	284, 590, 622, 815, 1249, 1525, 1776, 1875
Alw44I	GTGCAC	2 cut(s)	1446, 1470
Ama87I	CYCGRG	1 cut(s)	211
AoxI	GGCC	6 cut(s)	31, 958, 1104, 1484, 1674, 2359
ApaLI	GTGCAC	2 cut(s)	1446, 1470
ApoI	RAATTY	3 cut(s)	1025, 1098, 2332
Asp700I	GAANNNNTTC	3 cut(s)	1257, 2274, 2332
AspLEI	GCGC	2 cut(s)	698, 1961
AspS9I	GGNCC	1 cut(s)	861
AsuC2I	CCSGG	1 cut(s)	2401
AsuNHI	GCTAGC	1 cut(s)	757
AvaI	CYCGRG	1 cut(s)	211
AvaII	GGWCC	1 cut(s)	861
BaeGI	GKGCMC	2 cut(s)	1450, 1474
BaeI	ACNNNNGTAYC	2 cut(s)	268, 301
BamHI	GGATCC	2 cut(s)	834, 1768
BanI	GGYRCC	2 cut(s)	206, 741
BanII	GRGCYC	2 cut(s)	1752, 2439
BarI	GAAGNNNNNNTAC	2 cut(s)	1274, 1306
BbsI	GAAGAC	1 cut(s)	1584
Bbv12I	GWGCWC	4 cut(s)	1450, 1474, 1752, 2439
BccI	CCATC	5 cut(s)	71, 872, 1381, 2285, 2370
BceAI	ACGGC	3 cut(s)	836, 999, 1914
BcgI	CGANNNNNNTGC	2 cut(s)	217, 251
BciT130I	CCWGG	3 cut(s)	325, 700, 2475
BclI	TGATCA	3 cut(s)	51, 82, 97
BcnI	CCSGG	1 cut(s)	2401
BcoDI	GTCTC	8 cut(s)	284, 590, 622, 815, 1249, 1525, 1776, 1875
BfaI	CTAG	3 cut(s)	758, 1400, 2429
BfrI	CTTAAG	2 cut(s)	1595, 2203
BfuAI	ACCTGC	3 cut(s)	37, 762, 2441
Bme1390I	CCNGG	4 cut(s)	325, 700, 2401, 2475
Bme18I	GGWCC	1 cut(s)	861
BmeT110I	CYCGRG	1 cut(s)	211
BmgT120I	GGNCC	1 cut(s)	861
BmiI	GGNNCC	4 cut(s)	208, 743, 836, 1770
BmrFI	CCNGG	4 cut(s)	325, 700, 2401, 2475
BmsI	GCATC	8 cut(s)	80, 432, 551, 683, 1058, 1197, 1621, 1826
BmtI	GCTAGC	1 cut(s)	761
BpiI	GAAGAC	1 cut(s)	1584
BpmI	CTGGAG	2 cut(s)	256, 1380
Bpu10I	CCTNAGC	1 cut(s)	1625
BpuEI	CTTGAG	7 cut(s)	190, 677, 1138, 1231, 1549, 1739, 1838
BpuMI	CCSGG	1 cut(s)	2401
Bsa29I	ATCGAT	1 cut(s)	426
BsaBI	GATNNNNATC	3 cut(s)	81, 102, 929
BsaI	GGTCTC	1 cut(s)	590
BsaJI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1677, 2106, 2474
BsaWI	WCCGGW	2 cut(s)	1333, 2238
BsaXI	ACNNNNNCTCC	2 cut(s)	2145, 2175
Bsc4I	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1725, 1778
Bse118I	RCCGGY	1 cut(s)	1985
Bse1I	ACTGG	1 cut(s)	863
Bse3DI	GCAATG	1 cut(s)	1566
Bse8I	GATNNNNATC	3 cut(s)	81, 102, 929
BseBI	CCWGG	3 cut(s)	325, 700, 2475
BseCI	ATCGAT	1 cut(s)	426
BseDI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1677, 2106, 2474
BseGI	GGATG	3 cut(s)	1342, 2362, 2500
BseJI	GATNNNNATC	3 cut(s)	81, 102, 929
BseLI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1725, 1778
BseMI	GCAATG	1 cut(s)	1566
BseMII	CTCAG	5 cut(s)	333, 1122, 1625, 1639, 1647
BseNI	ACTGG	1 cut(s)	863
BseRI	GAGGAG	4 cut(s)	1742, 1841, 2389, 2447
BseSI	GKGCMC	2 cut(s)	1450, 1474
BseYI	CCCAGC	1 cut(s)	737
BsgI	GTGCAG	2 cut(s)	1530, 1719
BshFI	GGCC	6 cut(s)	33, 960, 1106, 1486, 1676, 2361
BshNI	GGYRCC	2 cut(s)	206, 741
BshVI	ATCGAT	1 cut(s)	426
BsiHKAI	GWGCWC	4 cut(s)	1450, 1474, 1752, 2439
BsiHKCI	CYCGRG	1 cut(s)	211
BsiSI	CCGG	4 cut(s)	1334, 1986, 2239, 2400
BslFI	GGGAC	2 cut(s)	1423, 1439
BslI	CCNNNNNNNGG	8 cut(s)	203, 279, 340, 779, 858, 1021, 1725, 1778
BsmAI	GTCTC	8 cut(s)	284, 590, 622, 815, 1249, 1525, 1776, 1875
BsmBI	CGTCTC	2 cut(s)	622, 815
BsmFI	GGGAC	2 cut(s)	1423, 1439
BsmI	GAATGC	1 cut(s)	1735
BsnI	GGCC	6 cut(s)	33, 960, 1106, 1486, 1676, 2361
Bso31I	GGTCTC	1 cut(s)	590
BsoBI	CYCGRG	1 cut(s)	211
Bsp1286I	GDGCHC	4 cut(s)	1450, 1474, 1752, 2439
Bsp19I	CCATGG	5 cut(s)	264, 915, 1020, 1677, 2106
BspACI	CCGC	3 cut(s)	773, 838, 1066
BspANI	GGCC	6 cut(s)	33, 960, 1106, 1486, 1676, 2361
BspCNI	CTCAG	5 cut(s)	332, 1123, 1626, 1638, 1648
BspDI	ATCGAT	1 cut(s)	426
BspHI	TCATGA	2 cut(s)	64, 94
BspLI	GGNNCC	4 cut(s)	208, 743, 836, 1770
BspMI	ACCTGC	3 cut(s)	37, 762, 2441
BspOI	GCTAGC	1 cut(s)	761
BspQI	GCTCTTC	1 cut(s)	1740
BspT107I	GGYRCC	2 cut(s)	206, 741
BspTI	CTTAAG	2 cut(s)	1595, 2203
BspTNI	GGTCTC	1 cut(s)	590
BsrDI	GCAATG	1 cut(s)	1566
BsrFI	RCCGGY	1 cut(s)	1985
BsrI	ACTGG	1 cut(s)	863
BssAI	RCCGGY	1 cut(s)	1985
BssECI	CCNNGG	9 cut(s)	264, 324, 778, 915, 955, 1020, 1677, 2106, 2474
BssT1I	CCWWGG	6 cut(s)	264, 778, 915, 1020, 1677, 2106
Bst2UI	CCWGG	3 cut(s)	325, 700, 2475
Bst4CI	ACNGT	7 cut(s)	720, 883, 1216, 2029, 2043, 2248, 2519
Bst6I	CTCTTC	2 cut(s)	650, 1740
BstAFI	CTTAAG	2 cut(s)	1595, 2203
BstAPI	GCANNNNNTGC	1 cut(s)	2056
BstC8I	GCNNGC	3 cut(s)	380, 759, 1720
BstDEI	CTNAG	8 cut(s)	319, 391, 994, 1082, 1131, 1625, 1634, 1656
BstDSI	CCRYGG	5 cut(s)	264, 915, 1020, 1677, 2106
BstEII	GGTNACC	1 cut(s)	893
BstF5I	GGATG	3 cut(s)	1342, 2362, 2500
BstHHI	GCGC	2 cut(s)	698, 1961
BstMAI	GTCTC	8 cut(s)	284, 590, 622, 815, 1249, 1525, 1776, 1875
BstMWI	GCNNNNNNNGC	3 cut(s)	320, 1919, 2056
BstNI	CCWGG	3 cut(s)	325, 700, 2475
BstNSI	RCATGY	5 cut(s)	946, 1432, 1811, 1877, 2063
BstPI	GGTNACC	1 cut(s)	893
BstSCI	CCNGG	4 cut(s)	323, 698, 2399, 2473
BstSLI	GKGCMC	2 cut(s)	1450, 1474
BstV2I	GAAGAC	1 cut(s)	1584
BstX2I	RGATCY	5 cut(s)	160, 834, 1630, 1768, 1944
BstYI	RGATCY	5 cut(s)	160, 834, 1630, 1768, 1944
Bsu15I	ATCGAT	1 cut(s)	426
BsuRI	GGCC	6 cut(s)	33, 960, 1106, 1486, 1676, 2361
BsuTUI	ATCGAT	1 cut(s)	426
BtgI	CCRYGG	5 cut(s)	264, 915, 1020, 1677, 2106
BtsCI	GGATG	3 cut(s)	1342, 2362, 2500
BtsI	GCAGTG	3 cut(s)	912, 1542, 1980
BtsIMutI	CAGTG	7 cut(s)	716, 912, 1542, 1980, 2253, 2261, 2391
BveI	ACCTGC	3 cut(s)	37, 762, 2441
Cac8I	GCNNGC	3 cut(s)	380, 759, 1720
CciI	TCATGA	2 cut(s)	64, 94
CfoI	GCGC	2 cut(s)	698, 1961
Cfr10I	RCCGGY	1 cut(s)	1985
Cfr13I	GGNCC	1 cut(s)	861
ClaI	ATCGAT	1 cut(s)	426
Csp6I	GTAC	8 cut(s)	136, 728, 748, 1017, 1291, 1847, 1978, 2096
CspCI	CAANNNNNGTGG	1 cut(s)	33
CviQI	GTAC	8 cut(s)	136, 728, 748, 1017, 1291, 1847, 1978, 2096
DdeI	CTNAG	8 cut(s)	319, 391, 994, 1082, 1131, 1625, 1634, 1656
DrdI	GACNNNNNNGTC	1 cut(s)	1417
DseDI	GACNNNNNNGTC	1 cut(s)	1417
Eam1104I	CTCTTC	2 cut(s)	650, 1740
EarI	CTCTTC	2 cut(s)	650, 1740
Ecl136II	GAGCTC	2 cut(s)	1750, 2437
Eco130I	CCWWGG	6 cut(s)	264, 778, 915, 1020, 1677, 2106
Eco147I	AGGCCT	1 cut(s)	1486
Eco24I	GRGCYC	2 cut(s)	1752, 2439
Eco31I	GGTCTC	1 cut(s)	590
Eco32I	GATATC	2 cut(s)	430, 512
Eco47I	GGWCC	1 cut(s)	861
Eco53kI	GAGCTC	2 cut(s)	1750, 2437
Eco57I	CTGAAG	1 cut(s)	2415
Eco88I	CYCGRG	1 cut(s)	211
Eco91I	GGTNACC	1 cut(s)	893
EcoICRI	GAGCTC	2 cut(s)	1750, 2437
EcoO65I	GGTNACC	1 cut(s)	893
EcoRI	GAATTC	1 cut(s)	2332
EcoRII	CCWGG	3 cut(s)	323, 698, 2473
EcoRV	GATATC	2 cut(s)	430, 512
EcoT14I	CCWWGG	6 cut(s)	264, 778, 915, 1020, 1677, 2106
EcoT38I	GRGCYC	2 cut(s)	1752, 2439
ErhI	CCWWGG	6 cut(s)	264, 778, 915, 1020, 1677, 2106
Esp3I	CGTCTC	2 cut(s)	622, 815
FalI	AAGNNNNNCTT	4 cut(s)	629, 661, 1215, 1247
FaqI	GGGAC	2 cut(s)	1423, 1439
FauI	CCCGC	1 cut(s)	780
FbaI	TGATCA	3 cut(s)	51, 82, 97
FblI	GTMKAC	2 cut(s)	855, 2124
FokI	GGATG	3 cut(s)	1349, 2349, 2507
FriOI	GRGCYC	2 cut(s)	1752, 2439
FspBI	CTAG	3 cut(s)	758, 1400, 2429
GlaI	GCGC	2 cut(s)	697, 1960
GsaI	CCCAGC	1 cut(s)	741
GsuI	CTGGAG	2 cut(s)	256, 1380
HaeIII	GGCC	6 cut(s)	33, 960, 1106, 1486, 1676, 2361
HapII	CCGG	4 cut(s)	1334, 1986, 2239, 2400
HhaI	GCGC	2 cut(s)	698, 1961
Hin6I	GCGC	2 cut(s)	696, 1959
HinP1I	GCGC	2 cut(s)	696, 1959
HincII	GTYRAC	3 cut(s)	1063, 1196, 2065
HindII	GTYRAC	3 cut(s)	1063, 1196, 2065
HindIII	AAGCTT	3 cut(s)	185, 1492, 2546
HinfI	GANTC	9 cut(s)	229, 269, 488, 826, 1093, 1533, 1784, 2066, 2275
HpaII	CCGG	4 cut(s)	1334, 1986, 2239, 2400
Hpy188I	TCNGA	9 cut(s)	76, 160, 614, 692, 818, 1506, 1635, 2025, 2285
Hpy99I	CGWCG	7 cut(s)	157, 160, 364, 367, 452, 1409, 2084
HpyAV	CCTTC	6 cut(s)	655, 998, 1276, 2029, 2202, 2495
HpyCH4III	ACNGT	7 cut(s)	720, 883, 1216, 2029, 2043, 2248, 2519
HpyF10VI	GCNNNNNNNGC	3 cut(s)	320, 1919, 2056
HpyF3I	CTNAG	8 cut(s)	319, 391, 994, 1082, 1131, 1625, 1634, 1656
HspAI	GCGC	2 cut(s)	696, 1959
Ksp22I	TGATCA	3 cut(s)	51, 82, 97
LguI	GCTCTTC	1 cut(s)	1740
LmnI	GCTCC	7 cut(s)	615, 797, 1170, 1755, 2153, 2434, 2504
LweI	GCATC	8 cut(s)	80, 432, 551, 683, 1058, 1197, 1621, 1826
MaeI	CTAG	3 cut(s)	758, 1400, 2429
MaeIII	GTNAC	5 cut(s)	712, 893, 1865, 1892, 2161
MfeI	CAATTG	1 cut(s)	1914
MflI	RGATCY	5 cut(s)	160, 834, 1630, 1768, 1944
MhlI	GDGCHC	4 cut(s)	1450, 1474, 1752, 2439
MlyI	GAGTC	7 cut(s)	223, 263, 482, 835, 1527, 1778, 2060
MmeI	TCCRAC	3 cut(s)	138, 1746, 2212
MroXI	GAANNNNTTC	3 cut(s)	1257, 2274, 2332
MseI	TTAA	4 cut(s)	1125, 1596, 2204, 2578
MslI	CAYNNNNRTG	2 cut(s)	872, 920
MspA1I	CMGCKG	1 cut(s)	2006
MspCI	CTTAAG	2 cut(s)	1595, 2203
MspI	CCGG	4 cut(s)	1334, 1986, 2239, 2400
MspR9I	CCNGG	4 cut(s)	325, 700, 2401, 2475
MunI	CAATTG	1 cut(s)	1914
Mva1269I	GAATGC	1 cut(s)	1735
MvaI	CCWGG	3 cut(s)	325, 700, 2475
MwoI	GCNNNNNNNGC	3 cut(s)	320, 1919, 2056
NciI	CCSGG	1 cut(s)	2401
NcoI	CCATGG	5 cut(s)	264, 915, 1020, 1677, 2106
NheI	GCTAGC	1 cut(s)	757
NlaIV	GGNNCC	4 cut(s)	208, 743, 836, 1770
NmeAIII	GCCGAG	2 cut(s)	936, 1484
NmuCI	GTSAC	3 cut(s)	712, 893, 2161
NspI	RCATGY	5 cut(s)	946, 1432, 1811, 1877, 2063
PaeR7I	CTCGAG	1 cut(s)	211
PagI	TCATGA	2 cut(s)	64, 94
PaqCI	CACCTGC	2 cut(s)	37, 2441
PceI	AGGCCT	1 cut(s)	1486
PciI	ACATGT	2 cut(s)	942, 1428
PciSI	GCTCTTC	1 cut(s)	1740
PctI	GAATGC	1 cut(s)	1735
PdmI	GAANNNNTTC	3 cut(s)	1257, 2274, 2332
PfeI	GAWTC	2 cut(s)	1093, 2275
PleI	GAGTC	7 cut(s)	223, 263, 482, 834, 1527, 1778, 2060
PpsI	GAGTC	7 cut(s)	223, 263, 482, 834, 1527, 1778, 2060
PscI	ACATGT	2 cut(s)	942, 1428
Psp124BI	GAGCTC	2 cut(s)	1752, 2439
Psp6I	CCWGG	3 cut(s)	323, 698, 2473
PspEI	GGTNACC	1 cut(s)	893
PspFI	CCCAGC	1 cut(s)	737
PspGI	CCWGG	3 cut(s)	323, 698, 2473
PspN4I	GGNNCC	4 cut(s)	208, 743, 836, 1770
PspPI	GGNCC	1 cut(s)	861
PspXI	VCTCGAGB	1 cut(s)	211
PsuI	RGATCY	5 cut(s)	160, 834, 1630, 1768, 1944
PvuII	CAGCTG	1 cut(s)	2006
RsaI	GTAC	8 cut(s)	137, 729, 749, 1018, 1292, 1848, 1979, 2097
RsaNI	GTAC	8 cut(s)	136, 728, 748, 1017, 1291, 1847, 1978, 2096
RseI	CAYNNNNRTG	2 cut(s)	872, 920
SacI	GAGCTC	2 cut(s)	1752, 2439
SapI	GCTCTTC	1 cut(s)	1740
SaqAI	TTAA	4 cut(s)	1125, 1596, 2204, 2578
Sau96I	GGNCC	1 cut(s)	861
SchI	GAGTC	7 cut(s)	223, 263, 482, 835, 1527, 1778, 2060
ScrFI	CCNGG	4 cut(s)	325, 700, 2401, 2475
SduI	GDGCHC	4 cut(s)	1450, 1474, 1752, 2439
SfaNI	GCATC	8 cut(s)	80, 432, 551, 683, 1058, 1197, 1621, 1826
Sfr274I	CTCGAG	1 cut(s)	211
SinI	GGWCC	1 cut(s)	861
SlaI	CTCGAG	1 cut(s)	211
SmiMI	CAYNNNNRTG	2 cut(s)	872, 920
SseBI	AGGCCT	1 cut(s)	1486
SsiI	CCGC	3 cut(s)	773, 838, 1066
SspI	AATATT	3 cut(s)	1650, 2446, 2487
SspMI	CTAG	3 cut(s)	758, 1400, 2429
SstI	GAGCTC	2 cut(s)	1752, 2439
StuI	AGGCCT	1 cut(s)	1486
StyD4I	CCNGG	4 cut(s)	323, 698, 2399, 2473
StyI	CCWWGG	6 cut(s)	264, 778, 915, 1020, 1677, 2106
TaaI	ACNGT	7 cut(s)	720, 883, 1216, 2029, 2043, 2248, 2519
TaqI	TCGA	8 cut(s)	127, 212, 426, 486, 518, 2079, 2469, 2523
TaqII	GACCGA	2 cut(s)	572, 780
TatI	WGTACW	5 cut(s)	135, 727, 747, 1290, 2095
TfiI	GAWTC	2 cut(s)	1093, 2275
Tru1I	TTAA	4 cut(s)	1125, 1596, 2204, 2578
Tru9I	TTAA	4 cut(s)	1125, 1596, 2204, 2578
TscAI	CASTG	7 cut(s)	723, 912, 1549, 1987, 2253, 2268, 2398
TseFI	GTSAC	3 cut(s)	712, 893, 2161
Tsp45I	GTSAC	3 cut(s)	712, 893, 2161
TspGWI	ACGGA	3 cut(s)	1965, 2097, 2507
TspRI	CASTG	7 cut(s)	723, 912, 1549, 1987, 2253, 2268, 2398
Vha464I	CTTAAG	2 cut(s)	1595, 2203
VneI	GTGCAC	2 cut(s)	1446, 1470
VpaK11BI	GGWCC	1 cut(s)	861
XapI	RAATTY	3 cut(s)	1025, 1098, 2332
XceI	RCATGY	5 cut(s)	946, 1432, 1811, 1877, 2063
XhoI	CTCGAG	1 cut(s)	211
XmiI	GTMKAC	2 cut(s)	855, 2124
XmnI	GAANNNNTTC	3 cut(s)	1257, 2274, 2332
XspI	CTAG	3 cut(s)	758, 1400, 2429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.