Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003447

Physical Location & Seq

Genomic Coordinates Reverse (-)

20082145 .. 20089839

Gene Structure 7695 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g28980.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGCAGAAAAAGGTTTGTAGCTGGAAATTAGGCCTAAATGGGCAGCAGAGGATGATGAGAGGTCATCATGAAGCTGATCAACATCACAGCAATTACAAGAACAATAATGATCAAATGACATCGTCTGATGAGCTCAACTTGAGAAACCTCTCAAAGCTTATACTCCCTCCTCTGGGCGCCTCAAGCTACAACCAGACTCAAATGCAATCAAAAGGCTATATTATTTCCCCCATGGACTCCAGATACAGGAGTTGGGAGGTGTTTATGGTGCTTTTGGTGGCTTACTCGGCCTGGGTTTACCCGTTTGAGGTTGCATTTCTCAACTCGACGACGGGAACAAGCATCAATCCTCTCTACTTAGCAGACTACATTGTTGATCTGTTTTTCGGCATCGATATCGTACTGACATTCTTCGTTGCGTACATCGATCCCAGAACTCAGTTGCTTGTTCTCGACTCCAATAAAATCGCAATAAGGTATCTTTCGACATGGTTCTTTATGGATTTGGCATCAACCCTCCCCTTCGAGGTTATAGGCAGACACAAATTGGGTCTCTCCTACTCTCTCTTGGGATTGCTCCGATTTTGGCGATTAAGACGAGCAAAGAAACTCTTCACAAGGCTTGAGAAGGACATCAGGTTTAGCTACTTCTGGGTCAGATGCGCCAGGCTTTTATCTGTGACACTGCTTTCAGTACATTGTGCTGGATGCCTGTACTACCTGCTAGCAGACCGATACCCACACGAAGGGAGGACATGGATTGGAGCTGTTATTCCAAATTTCAGAGAGACGAGTTTGTGGATTCGCTACATTTCAGCCCTCTACTGGTCCATCACCACCATGTCCACCGTCGGTTATGGTGACCTCCATGCAGTTAACACCATGGAAATGATCTTCATCATCTTATACATGCTTTTCAACCTTGGCCTTACTGCTTACCTAATCGGCAACATGACAAACTTAGTTGTGGAAGGCACTCGTCGTACCATGGAATTTAGAAACAGCGTTGAAGCAGCATCAAACTTTGTTAGCCGCAATCGCTTGCCTCCAAGGTTGAAGGATCAAATTCTGGCCTATATTTGCTTGAGATTCAAGGCTGAGAGCTTGAACCAGCAGCAAATAATTGAACAGCTCCCCAAGTCAATTTGTAAAAGCATCTGTCAATATTTATTCCTGCCAACAGTTGAGAAAGTTTATCTTTTCAAAGGTGTCTCCCGGGAAATACTTCTAATTCTGGTTGCAAACATGAAGGCAGAGTACATTCCACCAAGAGAAGATGTTGTAATGCAAAACGAAGCACCCGATGATATATACATCATTGTTTCTGGAGAAGTGGAAATCATTGATTGTGAGATGGATAAAGAGCTAGTCGTCGGGACTTTGAAATCTGGGGACATGTTTGGAGAAGTAGGTGCACTTTGTTGCCGAGAAGTTGGTGCACTTTGCTGTAGGCCTCAAAGCTTTACCTATCGAACGAAAACACTTTCACAACTCTTGAGACTCAAAACCACTGCACTAATAGAAGCAATGCGGACCAAACAAGAAGACAATGCTCAAATGCTTAAGAACTTCAAGCAGCATCACAGAAAGCTCAGGGTTCTGAAGCTTGGAGAAATATTGGTTGAGAGTGGGGAAGAAGAGGCCATGGGTGATTCGAATTTGGCATTCGACTTAATGACAGTTGCCAGCAAAGGGAATGCTGCACTTCTTGAAGAGCTTCTCAAGTCAAAGTTGGATCCTGACATTGGAGACTCCAAAGGAAGAACACCTCTGCATGTAGCAGCATCAAACGGACATGAAGAATGTGTCTTGGTACTCCTCAAGTATGCTTGTAACATTCATGTAAGAGCTTATATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0031984 GO:0033993 GO:0034220 GO:0042175 GO:0042221 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

620

Amino Acids

70.9

Weight (kDa)

8.66

Isoelectric Point (pI)

26.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	82 - 329	5e-42	Ion transport protein
Ion_trans_2	PF07885	252 - 323	1.2e-13	Ion channel
cNMP_binding	PF00027	420 - 512	8.1e-11	Cyclic nucleotide-binding domain
Ank_2	PF12796	558 - 639	5.5e-16	Ankyrin repeats (3 copies)
Ank_4	PF13637	562 - 607	1.4e-06	Ankyrin repeats (many copies)
Ank_5	PF13857	578 - 624	5.3e-08	Ankyrin repeats (many copies)
Ank	PF00023	587 - 617	9.5e-08	Ankyrin repeat
Ank_2	PF12796	654 - 711	1.3e-08	Ankyrin repeats (3 copies)
KHA	PF11834	770 - 841	6.1e-22	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22200 AT4G22200
fragaria_vesca	FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980
malus_domestica	MD08G1236000.v1.1
prunus_persica	Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1
pyrus_communis	pycom08g20420
rosa_chinensis	RchiOBHm_Chr7g0221301 RchiOBHm_Chr7g0222541
rosa_laevigata	RLG00000002163
rosa_multiflora	Rmu_co8307231.1_g000001 Rmu_sc0000324.1_g000021
rosa_roxburghii	Rroxscaffold_3G00238430
rosa_rugosa	Rorug07G0202000
rosa_samantha	Rh6BG194600 Rh7AG341200 Rh7AG352200 Rh7BG332200 Rh7CG359100 Rh7CG369500 Rh7DG339700
rosa_wichuraiana	Rw7G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	729
AccB1I	GGYRCC	1 cut(s)	176
AciI	CCGC	2 cut(s)	1033, 1531
AclWI	GGATC	4 cut(s)	422, 1068, 1730, 1743
AcsI	RAATTY	4 cut(s)	778, 992, 1065, 1657
AcuI	CTGAAG	1 cut(s)	1622
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	7 cut(s)	402, 422, 696, 716, 985, 1259, 1815
AfiI	CCNNNNNNNGG	9 cut(s)	172, 173, 246, 307, 526, 746, 825, 1692, 1745
AflII	CTTAAG	1 cut(s)	1562
AflIII	ACRYGT	1 cut(s)	1395
AjnI	CCWGG	2 cut(s)	290, 665
Alw21I	GWGCWC	3 cut(s)	135, 1417, 1441
Alw26I	GTCTC	5 cut(s)	557, 782, 1216, 1492, 1743
Alw44I	GTGCAC	2 cut(s)	1413, 1437
AlwI	GGATC	4 cut(s)	422, 1068, 1730, 1743
Ama87I	CYCGRG	1 cut(s)	1215
AoxI	GGCC	6 cut(s)	31, 288, 925, 1071, 1451, 1641
ApaLI	GTGCAC	2 cut(s)	1413, 1437
ApeKI	GCWGC	6 cut(s)	43, 1013, 1114, 1576, 1700, 1781
ApoI	RAATTY	4 cut(s)	778, 992, 1065, 1657
Asp700I	GAANNNNTTC	3 cut(s)	611, 1224, 1716
AspLEI	GCGC	2 cut(s)	179, 665
AspS9I	GGNCC	2 cut(s)	828, 1533
AsuC2I	CCSGG	2 cut(s)	1216, 1217
AsuHPI	GGTGA	3 cut(s)	826, 872, 1661
AsuII	TTCGAA	1 cut(s)	1655
AsuNHI	GCTAGC	1 cut(s)	724
AvaI	CYCGRG	1 cut(s)	1215
AvaII	GGWCC	2 cut(s)	828, 1533
BaeGI	GKGCMC	2 cut(s)	1417, 1441
BaeI	ACNNNNGTAYC	2 cut(s)	235, 268
BamHI	GGATCC	1 cut(s)	1735
BanI	GGYRCC	1 cut(s)	176
BanII	GRGCYC	1 cut(s)	135
BarI	GAAGNNNNNNTAC	2 cut(s)	1241, 1273
BbsI	GAAGAC	1 cut(s)	1551
Bbv12I	GWGCWC	3 cut(s)	135, 1417, 1441
BbvI	GCAGC	6 cut(s)	55, 1025, 1126, 1588, 1687, 1793
BccI	CCATC	2 cut(s)	839, 1348
BcgI	CGANNNNNNTGC	2 cut(s)	379, 413
BciT130I	CCWGG	2 cut(s)	292, 667
BclI	TGATCA	2 cut(s)	76, 109
BcnI	CCSGG	2 cut(s)	1216, 1217
BcoDI	GTCTC	5 cut(s)	557, 782, 1216, 1492, 1743
BfaI	CTAG	2 cut(s)	725, 1367
BfmI	CTRYAG	1 cut(s)	1447
BfoI	RGCGCY	1 cut(s)	180
BfrI	CTTAAG	1 cut(s)	1562
BfuAI	ACCTGC	1 cut(s)	729
BisI	GCNGC	7 cut(s)	44, 1014, 1033, 1115, 1577, 1701, 1782
BlsI	GCNGC	7 cut(s)	45, 1015, 1034, 1116, 1578, 1702, 1783
Bme1390I	CCNGG	4 cut(s)	292, 667, 1216, 1217
Bme18I	GGWCC	2 cut(s)	828, 1533
BmeT110I	CYCGRG	1 cut(s)	1215
BmgT120I	GGNCC	2 cut(s)	828, 1533
BmiI	GGNNCC	2 cut(s)	178, 1737
BmrFI	CCNGG	4 cut(s)	292, 667, 1216, 1217
BmsI	GCATC	9 cut(s)	351, 399, 518, 650, 698, 1025, 1164, 1588, 1793
BmtI	GCTAGC	1 cut(s)	728
BpiI	GAAGAC	1 cut(s)	1551
BpmI	CTGGAG	2 cut(s)	223, 1347
Bpu10I	CCTNAGC	1 cut(s)	1592
Bpu14I	TTCGAA	1 cut(s)	1655
BpuEI	CTTGAG	7 cut(s)	160, 166, 644, 1105, 1516, 1706, 1805
BpuMI	CCSGG	2 cut(s)	1216, 1217
Bsa29I	ATCGAT	2 cut(s)	393, 426
BsaBI	GATNNNNATC	2 cut(s)	81, 896
BsaHI	GRCGYC	1 cut(s)	177
BsaI	GGTCTC	1 cut(s)	557
BsaJI	CCNNGG	8 cut(s)	231, 291, 882, 922, 987, 1049, 1215, 1644
Bsc4I	CCNNNNNNNGG	9 cut(s)	172, 173, 246, 307, 526, 746, 825, 1692, 1745
Bse1I	ACTGG	1 cut(s)	830
Bse3DI	GCAATG	1 cut(s)	1533
Bse8I	GATNNNNATC	2 cut(s)	81, 896
BseBI	CCWGG	2 cut(s)	292, 667
BseCI	ATCGAT	2 cut(s)	393, 426
BseDI	CCNNGG	8 cut(s)	231, 291, 882, 922, 987, 1049, 1215, 1644
BseGI	GGATG	2 cut(s)	57, 713
BseJI	GATNNNNATC	2 cut(s)	81, 896
BseLI	CCNNNNNNNGG	9 cut(s)	172, 173, 246, 307, 526, 746, 825, 1692, 1745
BseMI	GCAATG	1 cut(s)	1533
BseMII	CTCAG	3 cut(s)	452, 1089, 1606
BseNI	ACTGG	1 cut(s)	830
BseRI	GAGGAG	2 cut(s)	159, 1808
BseSI	GKGCMC	2 cut(s)	1417, 1441
BseXI	GCAGC	6 cut(s)	55, 1025, 1126, 1588, 1687, 1793
BsgI	GTGCAG	2 cut(s)	1497, 1686
BshFI	GGCC	6 cut(s)	33, 290, 927, 1073, 1453, 1643
BshNI	GGYRCC	1 cut(s)	176
BshVI	ATCGAT	2 cut(s)	393, 426
BsiHKAI	GWGCWC	3 cut(s)	135, 1417, 1441
BsiHKCI	CYCGRG	1 cut(s)	1215
BsiSI	CCGG	1 cut(s)	1216
BslFI	GGGAC	2 cut(s)	1390, 1406
BslI	CCNNNNNNNGG	9 cut(s)	172, 173, 246, 307, 526, 746, 825, 1692, 1745
BsmAI	GTCTC	5 cut(s)	557, 782, 1216, 1492, 1743
BsmBI	CGTCTC	1 cut(s)	782
BsmFI	GGGAC	2 cut(s)	1390, 1406
BsmI	GAATGC	2 cut(s)	1664, 1702
BsnI	GGCC	6 cut(s)	33, 290, 927, 1073, 1453, 1643
Bso31I	GGTCTC	1 cut(s)	557
BsoBI	CYCGRG	1 cut(s)	1215
Bsp119I	TTCGAA	1 cut(s)	1655
Bsp1286I	GDGCHC	3 cut(s)	135, 1417, 1441
Bsp143I	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
Bsp19I	CCATGG	4 cut(s)	231, 882, 987, 1644
BspACI	CCGC	2 cut(s)	1033, 1531
BspANI	GGCC	6 cut(s)	33, 290, 927, 1073, 1453, 1643
BspCNI	CTCAG	3 cut(s)	451, 1090, 1605
BspDI	ATCGAT	2 cut(s)	393, 426
BspHI	TCATGA	1 cut(s)	67
BspLI	GGNNCC	2 cut(s)	178, 1737
BspMI	ACCTGC	1 cut(s)	729
BspOI	GCTAGC	1 cut(s)	728
BspPI	GGATC	4 cut(s)	422, 1068, 1730, 1743
BspQI	GCTCTTC	1 cut(s)	1707
BspT104I	TTCGAA	1 cut(s)	1655
BspT107I	GGYRCC	1 cut(s)	176
BspTI	CTTAAG	1 cut(s)	1562
BspTNI	GGTCTC	1 cut(s)	557
BsrDI	GCAATG	1 cut(s)	1533
BsrI	ACTGG	1 cut(s)	830
BssECI	CCNNGG	8 cut(s)	231, 291, 882, 922, 987, 1049, 1215, 1644
BssMI	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
BssNI	GRCGYC	1 cut(s)	177
BssT1I	CCWWGG	6 cut(s)	231, 882, 922, 987, 1049, 1644
Bst2UI	CCWGG	2 cut(s)	292, 667
Bst4CI	ACNGT	3 cut(s)	850, 1183, 1681
Bst6I	CTCTTC	3 cut(s)	617, 1632, 1707
BstACI	GRCGYC	1 cut(s)	177
BstAFI	CTTAAG	1 cut(s)	1562
BstBI	TTCGAA	1 cut(s)	1655
BstC8I	GCNNGC	3 cut(s)	726, 1043, 1687
BstDEI	CTNAG	5 cut(s)	358, 438, 961, 1098, 1592
BstDSI	CCRYGG	4 cut(s)	231, 882, 987, 1644
BstEII	GGTNACC	1 cut(s)	860
BstF5I	GGATG	2 cut(s)	57, 713
BstH2I	RGCGCY	1 cut(s)	180
BstHHI	GCGC	2 cut(s)	179, 665
BstKTI	GATC	7 cut(s)	79, 112, 379, 430, 894, 1063, 1738
BstMAI	GTCTC	5 cut(s)	557, 782, 1216, 1492, 1743
BstMBI	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
BstMWI	GCNNNNNNNGC	3 cut(s)	183, 287, 1038
BstNI	CCWGG	2 cut(s)	292, 667
BstNSI	RCATGY	3 cut(s)	913, 1399, 1778
BstPI	GGTNACC	1 cut(s)	860
BstSCI	CCNGG	4 cut(s)	290, 665, 1214, 1215
BstSFI	CTRYAG	1 cut(s)	1447
BstSLI	GKGCMC	2 cut(s)	1417, 1441
BstV1I	GCAGC	6 cut(s)	55, 1025, 1126, 1588, 1687, 1793
BstV2I	GAAGAC	1 cut(s)	1551
BstX2I	RGATCY	1 cut(s)	1735
BstYI	RGATCY	1 cut(s)	1735
Bsu15I	ATCGAT	2 cut(s)	393, 426
BsuRI	GGCC	6 cut(s)	33, 290, 927, 1073, 1453, 1643
BsuTUI	ATCGAT	2 cut(s)	393, 426
BtgI	CCRYGG	4 cut(s)	231, 882, 987, 1644
BtsCI	GGATG	2 cut(s)	57, 713
BtsI	GCAGTG	2 cut(s)	683, 1509
BtsIMutI	CAGTG	2 cut(s)	683, 1509
BveI	ACCTGC	1 cut(s)	729
Cac8I	GCNNGC	3 cut(s)	726, 1043, 1687
CciI	TCATGA	1 cut(s)	67
CfoI	GCGC	2 cut(s)	179, 665
Cfr13I	GGNCC	2 cut(s)	828, 1533
Cfr9I	CCCGGG	1 cut(s)	1215
ClaI	ATCGAT	2 cut(s)	393, 426
Csp6I	GTAC	7 cut(s)	401, 421, 695, 715, 984, 1258, 1814
CviQI	GTAC	7 cut(s)	401, 421, 695, 715, 984, 1258, 1814
DdeI	CTNAG	5 cut(s)	358, 438, 961, 1098, 1592
DinI	GGCGCC	1 cut(s)	178
DpnI	GATC	7 cut(s)	78, 111, 378, 429, 893, 1062, 1737
DpnII	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
Eam1104I	CTCTTC	3 cut(s)	617, 1632, 1707
EarI	CTCTTC	3 cut(s)	617, 1632, 1707
Ecl136II	GAGCTC	1 cut(s)	133
Eco130I	CCWWGG	6 cut(s)	231, 882, 922, 987, 1049, 1644
Eco147I	AGGCCT	2 cut(s)	33, 1453
Eco24I	GRGCYC	1 cut(s)	135
Eco31I	GGTCTC	1 cut(s)	557
Eco32I	GATATC	1 cut(s)	397
Eco47I	GGWCC	2 cut(s)	828, 1533
Eco53kI	GAGCTC	1 cut(s)	133
Eco57I	CTGAAG	1 cut(s)	1622
Eco88I	CYCGRG	1 cut(s)	1215
Eco91I	GGTNACC	1 cut(s)	860
EcoICRI	GAGCTC	1 cut(s)	133
EcoO65I	GGTNACC	1 cut(s)	860
EcoRII	CCWGG	2 cut(s)	290, 665
EcoRV	GATATC	1 cut(s)	397
EcoT14I	CCWWGG	6 cut(s)	231, 882, 922, 987, 1049, 1644
EcoT38I	GRGCYC	1 cut(s)	135
EgeI	GGCGCC	1 cut(s)	178
EheI	GGCGCC	1 cut(s)	178
ErhI	CCWWGG	6 cut(s)	231, 882, 922, 987, 1049, 1644
Esp3I	CGTCTC	1 cut(s)	782
FalI	AAGNNNNNCTT	4 cut(s)	596, 628, 1182, 1214
FaqI	GGGAC	2 cut(s)	1390, 1406
FbaI	TGATCA	2 cut(s)	76, 109
Fnu4HI	GCNGC	7 cut(s)	44, 1014, 1033, 1115, 1577, 1701, 1782
FokI	GGATG	2 cut(s)	64, 720
FriOI	GRGCYC	1 cut(s)	135
Fsp4HI	GCNGC	7 cut(s)	44, 1014, 1033, 1115, 1577, 1701, 1782
FspBI	CTAG	2 cut(s)	725, 1367
GlaI	GCGC	2 cut(s)	178, 664
GluI	GCNGC	7 cut(s)	44, 1014, 1033, 1115, 1577, 1701, 1782
GsuI	CTGGAG	2 cut(s)	223, 1347
HaeII	RGCGCY	1 cut(s)	180
HaeIII	GGCC	6 cut(s)	33, 290, 927, 1073, 1453, 1643
HapII	CCGG	1 cut(s)	1216
HhaI	GCGC	2 cut(s)	179, 665
Hin1I	GRCGYC	1 cut(s)	177
Hin6I	GCGC	2 cut(s)	177, 663
HinP1I	GCGC	2 cut(s)	177, 663
HincII	GTYRAC	1 cut(s)	877
HindII	GTYRAC	1 cut(s)	877
HindIII	AAGCTT	3 cut(s)	155, 1459, 1604
HinfI	GANTC	8 cut(s)	196, 236, 455, 802, 1089, 1500, 1652, 1751
HpaI	GTTAAC	1 cut(s)	877
HpaII	CCGG	1 cut(s)	1216
HphI	GGTGA	3 cut(s)	826, 872, 1661
Hpy166II	GTNNAC	5 cut(s)	298, 846, 877, 1415, 1439
Hpy188I	TCNGA	5 cut(s)	127, 581, 659, 785, 1602
Hpy188III	TCNNGA	8 cut(s)	68, 240, 452, 1326, 1375, 1495, 1709, 1739
Hpy8I	GTNNAC	5 cut(s)	298, 846, 877, 1415, 1439
Hpy99I	CGWCG	5 cut(s)	331, 334, 854, 984, 1376
HpyAV	CCTTC	6 cut(s)	532, 622, 740, 965, 1051, 1243
HpyCH4III	ACNGT	3 cut(s)	850, 1183, 1681
HpyF10VI	GCNNNNNNNGC	3 cut(s)	183, 287, 1038
HpyF3I	CTNAG	5 cut(s)	358, 438, 961, 1098, 1592
Hsp92I	GRCGYC	1 cut(s)	177
HspAI	GCGC	2 cut(s)	177, 663
KasI	GGCGCC	1 cut(s)	176
Ksp22I	TGATCA	2 cut(s)	76, 109
KspAI	GTTAAC	1 cut(s)	877
Kzo9I	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
LguI	GCTCTTC	1 cut(s)	1707
LmnI	GCTCC	3 cut(s)	582, 764, 1137
Lsp1109I	GCAGC	6 cut(s)	55, 1025, 1126, 1588, 1687, 1793
LweI	GCATC	9 cut(s)	351, 399, 518, 650, 698, 1025, 1164, 1588, 1793
MaeI	CTAG	2 cut(s)	725, 1367
MaeIII	GTNAC	3 cut(s)	679, 860, 1832
MalI	GATC	7 cut(s)	78, 111, 378, 429, 893, 1062, 1737
MboI	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
MflI	RGATCY	1 cut(s)	1735
MhlI	GDGCHC	3 cut(s)	135, 1417, 1441
Mly113I	GGCGCC	1 cut(s)	177
MlyI	GAGTC	5 cut(s)	190, 230, 449, 1494, 1745
MmeI	TCCRAC	1 cut(s)	1713
MroXI	GAANNNNTTC	3 cut(s)	611, 1224, 1716
MseI	TTAA	4 cut(s)	593, 876, 1563, 1673
MslI	CAYNNNNRTG	2 cut(s)	839, 887
MspCI	CTTAAG	1 cut(s)	1562
MspI	CCGG	1 cut(s)	1216
MspR9I	CCNGG	4 cut(s)	292, 667, 1216, 1217
Mva1269I	GAATGC	2 cut(s)	1664, 1702
MvaI	CCWGG	2 cut(s)	292, 667
MwoI	GCNNNNNNNGC	3 cut(s)	183, 287, 1038
NarI	GGCGCC	1 cut(s)	177
NciI	CCSGG	2 cut(s)	1216, 1217
NcoI	CCATGG	4 cut(s)	231, 882, 987, 1644
NdeII	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
NheI	GCTAGC	1 cut(s)	724
NlaIV	GGNNCC	2 cut(s)	178, 1737
NmeAIII	GCCGAG	2 cut(s)	266, 1451
NmuCI	GTSAC	2 cut(s)	679, 860
NspI	RCATGY	3 cut(s)	913, 1399, 1778
NspV	TTCGAA	1 cut(s)	1655
PagI	TCATGA	1 cut(s)	67
PceI	AGGCCT	2 cut(s)	33, 1453
PciI	ACATGT	1 cut(s)	1395
PciSI	GCTCTTC	1 cut(s)	1707
PcsI	WCGNNNNNNNCGW	1 cut(s)	1299
PctI	GAATGC	2 cut(s)	1664, 1702
PdmI	GAANNNNTTC	3 cut(s)	611, 1224, 1716
PfeI	GAWTC	3 cut(s)	802, 1089, 1652
PflFI	GACNNNGTC	1 cut(s)	121
PkrI	GCNGC	7 cut(s)	45, 1015, 1034, 1116, 1578, 1702, 1783
PleI	GAGTC	5 cut(s)	190, 230, 449, 1494, 1745
PluTI	GGCGCC	1 cut(s)	180
PpsI	GAGTC	5 cut(s)	190, 230, 449, 1494, 1745
PscI	ACATGT	1 cut(s)	1395
Psp124BI	GAGCTC	1 cut(s)	135
Psp6I	CCWGG	2 cut(s)	290, 665
PspEI	GGTNACC	1 cut(s)	860
PspGI	CCWGG	2 cut(s)	290, 665
PspN4I	GGNNCC	2 cut(s)	178, 1737
PspPI	GGNCC	2 cut(s)	828, 1533
PsuI	RGATCY	1 cut(s)	1735
PsyI	GACNNNGTC	1 cut(s)	121
RsaI	GTAC	7 cut(s)	402, 422, 696, 716, 985, 1259, 1815
RsaNI	GTAC	7 cut(s)	401, 421, 695, 715, 984, 1258, 1814
RseI	CAYNNNNRTG	2 cut(s)	839, 887
SacI	GAGCTC	1 cut(s)	135
SapI	GCTCTTC	1 cut(s)	1707
SaqAI	TTAA	4 cut(s)	593, 876, 1563, 1673
SatI	GCNGC	7 cut(s)	44, 1014, 1033, 1115, 1577, 1701, 1782
Sau3AI	GATC	7 cut(s)	76, 109, 376, 427, 891, 1060, 1735
Sau96I	GGNCC	2 cut(s)	828, 1533
SchI	GAGTC	5 cut(s)	190, 230, 449, 1494, 1745
ScrFI	CCNGG	4 cut(s)	292, 667, 1216, 1217
SduI	GDGCHC	3 cut(s)	135, 1417, 1441
SfaNI	GCATC	9 cut(s)	351, 399, 518, 650, 698, 1025, 1164, 1588, 1793
SfcI	CTRYAG	1 cut(s)	1447
SfoI	GGCGCC	1 cut(s)	178
SfuI	TTCGAA	1 cut(s)	1655
SinI	GGWCC	2 cut(s)	828, 1533
SmaI	CCCGGG	1 cut(s)	1217
SmiMI	CAYNNNNRTG	2 cut(s)	839, 887
SmlI	CTYRAG	8 cut(s)	139, 181, 623, 1084, 1495, 1562, 1721, 1820
SmoI	CTYRAG	8 cut(s)	139, 181, 623, 1084, 1495, 1562, 1721, 1820
SseBI	AGGCCT	2 cut(s)	33, 1453
SsiI	CCGC	2 cut(s)	1033, 1531
SspDI	GGCGCC	1 cut(s)	176
SspI	AATATT	2 cut(s)	1166, 1617
SspMI	CTAG	2 cut(s)	725, 1367
SstI	GAGCTC	1 cut(s)	135
StuI	AGGCCT	2 cut(s)	33, 1453
StyD4I	CCNGG	4 cut(s)	290, 665, 1214, 1215
StyI	CCWWGG	6 cut(s)	231, 882, 922, 987, 1049, 1644
TaaI	ACNGT	3 cut(s)	850, 1183, 1681
TaqI	TCGA	9 cut(s)	326, 393, 426, 453, 485, 525, 1471, 1655, 1668
TaqII	GACCGA	1 cut(s)	747
TatI	WGTACW	3 cut(s)	694, 714, 1257
TauI	GCSGC	1 cut(s)	1035
TfiI	GAWTC	3 cut(s)	802, 1089, 1652
Tru1I	TTAA	4 cut(s)	593, 876, 1563, 1673
Tru9I	TTAA	4 cut(s)	593, 876, 1563, 1673
TscAI	CASTG	2 cut(s)	690, 1516
TseFI	GTSAC	2 cut(s)	679, 860
TseI	GCWGC	6 cut(s)	43, 1013, 1114, 1576, 1700, 1781
Tsp45I	GTSAC	2 cut(s)	679, 860
TspDTI	ATGAA	5 cut(s)	84, 886, 1262, 1812, 1829
TspGWI	ACGGA	1 cut(s)	1806
TspMI	CCCGGG	1 cut(s)	1215
TspRI	CASTG	2 cut(s)	690, 1516
Tth111I	GACNNNGTC	1 cut(s)	121
Vha464I	CTTAAG	1 cut(s)	1562
VneI	GTGCAC	2 cut(s)	1413, 1437
VpaK11BI	GGWCC	2 cut(s)	828, 1533
XapI	RAATTY	4 cut(s)	778, 992, 1065, 1657
XceI	RCATGY	3 cut(s)	913, 1399, 1778
XmaI	CCCGGG	1 cut(s)	1215
XmnI	GAANNNNTTC	3 cut(s)	611, 1224, 1716
XspI	CTAG	2 cut(s)	725, 1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.