Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0003447

Physical Location & Seq

Genomic Coordinates Forward (+)

30377633 .. 30385417

Gene Structure 7785 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1236000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2610 bp

ATGGAGTTTTTGAAGTTTAATCCCCACAAGAATCTTGATCATCCGTATCACCAACCAGCAGCAGCTGCCTCTTACAACATGAAGAAGGTCTGCAGCTGGAATAATCATACGCTTGAGCAGCACCACCAGCAGGTGCAGCAGCATAAAAAAAGTCATGAAAACGTGAACCCTTCGTCGTCGTCGTCTTCGTCGGAGCTTGATTTAAGAAACATTTCAAAGCTTATCCTTCCTCCCTTGGGTGCTTCGAGTTACAACCAAACTCCTATCCAGTCAAATGGATGGATTGTCTCGCCCATGGATTCAAGATACAGGTGTTGGGAAACATTCATGGTGGTTCTGGTGGCATACTCGGCGTGGGTTTACCCTTTTGAGGTTGCATTTCTTAACACGTCCAAAAGCAGGCCACTATACATTGCAGACAACGTCGTCGATCTATTTTTTGCCGCAGATATTATCTTGACATTCTTCGTCGCATATGTTGATCCAAGAACTCAGTTACTTGTTCATGACTCTAAAAAAATCGCCATGAGATACCTTACGACATGGTTTTTGATGGATTTGGCATCAACACTGCCATTTGAGGCACTTTTTTTAGCACTGGGAAAAAACAAATTGAGTGCCTCTTACTCTCTATTGGGATTGCTCAGATTCTGGCGACTAAGACGAGCAAAGCAACTCTTCACAAGACTGGAAAAGGATATCAGGTTTAGCTATTTTTGGGTCAGATGTGCAAGGCTTTTATCTGTAACAGTGTTTTCAGTACACTGTGCTGGATGCCTGTACTACCTGCTCGCAGATCGATATCCACACCGAGGGAAGACGTGGATTGGAACTGTGTTTCCAAATTTCAGAGAAACAGGCCTCTGGATCCGATACATCTCAGCCCTATACTGGTCTATGACCACCATGAGCACCGTCGGCTACGGCGATCTCCACGCAGTTAACACCGTCGAAATGATCTTCATTATTTTCTACATGCTCTTCAACCTTGGCCTCACTGCTTACTTGATCGGCAACATGACCAACTTGGTTGTTGAAGGCACTCGTCGTACCATGGAATTCAGAAGTAGCATTGAAGCAGCATCAAGTTTTGTGTGCCGGAATCGGTTGCCTCAACGGCTGAGGGACCAAATATTGGCATATATTTGCTTGAGATTTAAGGCGGAGAGCTTGAACCAGCAGCAGATGCTTGAACAGTTTCCCAAATCCATTTGCAAAAGCATATGCCAACATCTGTTTTTGCCAATTGTGGAAAAAGTATATCTTTTCAGAGGTGTTTCAAGGGAAACACTTCTGCATCTGGTTGCGAAGATGAAGGCAGAGTACATACCTCCTAGAGAAGATGTTGTGCTGCAAAGCGAAGCGCCGGAGGATGTTTATATCATTGTTTCTGGAGAAGTGGAAATAATTGATTGTGAGATGGAGAGAGAACGAGCTGTTGGGACTTTGCAATCTGGAGACATGGTTGGAGAAGTTACTGCATTTTGTGGTATAGAAGTTGGTAAATTATGCAGACCTCAAACCTTTACGTACCGAACCAAAACACTGTCACAGCTCTTGAGAATAAAAACCACTGCCCTAGTAGAAGCAATGCAGACCAAACCAGAGGACAAAGTACAAATGCTTAAAAACTTCAACCAGCATCACAAAAAGCTTAGGGATCTGAGAGTCGGAGAAATACTGGTTGAGAGTGGAGAGGACGAAGGTGATGACCCAAAGGCGGCATTCAACATGTTGACTGCTGCCAGCAAAGGCGATGCTACTCTTCTTGATGAGCTTCTCAAGGCAAAGTTGGATCCCGATATTGGAGACTTCAAGGGAAGAACTCCCCTGCACATAGCAGCATCAAAAGGCCACTACGAATGTGTTTTGGTACTCCTGAAGTATGCTTGTAACGTACATGTAAGAGATACAAATGGTAACACAGCGTTATGGGATGCCATTGCATCAAAGCACCATTCCATATTCCGAAGTCTCTACTTTTGTGCAGCCATATCTGATCCATACACCGCTGGCGATCTTCTATGCACTGCTGCCAAGAGAAATAATCTAACCGTAATGGAGGAACTCTTGAAGCAGGGATTAAATGTCGACTCAAAGAATCAGCTTGGGAAAACAGCCGTACAAATAGCCATGGAAGAAAACAATGCAGACATGGTAAATTTGCTAGTGATGAACGGTGCAGATGTTACCAATGCACAGAACTGCTTATTTCCTTCACAAAGTTTGACTGAAATGCTACAAAAACGAGAGGTTGGACACCGGATTACAGTTCCAGACAGCACAATAAATGAAGTGCCCTTGATGAAGAATGGAGAGCGTAATTCTGGGAGAGAATCCTATGGAGTAAATTGTCCAAGAGTGAGCATATACAGAGGCCATCCTATGGTTAGGAAAACAACATGTTGCAGGGAAGCTGGTAGAGTAATTAGATTGCCTCGTTCGCTAGAGGAACTCCGAAACGTTGCAGGTGTAAAGTTTGGATTTGATTCAGGAAATATATTGGTTACAGATGAAGGAGGAGCGGAAATTGACTCAATAGAAGTGATTCGAGATAATGACAAACTTTTCCTAGTTGAACTTGAAGACCCAAAGTCTGTCAAACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009506 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0012505 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0031984 GO:0033993 GO:0034220 GO:0042175 GO:0042221 GO:0042391 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0097305 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 Ion_trans KHA cNMP_binding

Protein Analysis

870

Amino Acids

98.68

Weight (kDa)

8.42

Isoelectric Point (pI)

34.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	103 - 351	1.7e-44	Ion transport protein
Ion_trans_2	PF07885	277 - 345	2.3e-13	Ion channel
cNMP_binding	PF00027	443 - 533	1.2e-09	Cyclic nucleotide-binding domain
Ank_2	PF12796	580 - 660	1.6e-14	Ankyrin repeats (3 copies)
Ank_4	PF13637	580 - 627	7.8e-06	Ankyrin repeats (many copies)
Ank_5	PF13857	593 - 644	1.7e-10	Ankyrin repeats (many copies)
Ank	PF00023	607 - 637	5.9e-08	Ankyrin repeat
Ank_2	PF12796	674 - 731	1e-07	Ankyrin repeats (3 copies)
KHA	PF11834	787 - 858	6.2e-21	KHA, dimerisation domain of potassium ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22200 AT4G22200
fragaria_vesca	FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980 FvH4_5g28980
malus_domestica	MD08G1236000.v1.1
prunus_persica	Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1 Prupe.1G572200_v2.0.a1
pyrus_communis	pycom08g20420
rosa_chinensis	RchiOBHm_Chr7g0221301 RchiOBHm_Chr7g0222541
rosa_laevigata	RLG00000002163
rosa_multiflora	Rmu_co8307231.1_g000001 Rmu_sc0000324.1_g000021
rosa_roxburghii	Rroxscaffold_3G00238430
rosa_rugosa	Rorug07G0202000
rosa_samantha	Rh6BG194600 Rh7AG341200 Rh7AG352200 Rh7BG332200 Rh7CG359100 Rh7CG369500 Rh7DG339700
rosa_wichuraiana	Rw7G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	121, 2459
AasI	GACNNNNNNGTC	1 cut(s)	425
Acc36I	ACCTGC	3 cut(s)	121, 795, 2459
AccB7I	CCANNNNNTGG	2 cut(s)	1135, 2386
AccBSI	CCGCTC	1 cut(s)	2525
AccI	GTMKAC	1 cut(s)	2091
AciI	CCGC	5 cut(s)	444, 1163, 1721, 2010, 2525
AclI	AACGTT	1 cut(s)	2463
AclWI	GGATC	7 cut(s)	476, 862, 875, 1668, 1790, 1803, 1994
AcsI	RAATTY	3 cut(s)	844, 1058, 2161
AcuI	CTGAAG	1 cut(s)	1901
AfaI	GTAC	9 cut(s)	762, 782, 1051, 1325, 1532, 1617, 1875, 1899, 2124
AflIII	ACRYGT	4 cut(s)	387, 1731, 1900, 2402
AhdI	GACNNNNNGTC	1 cut(s)	2593
AjiI	CACGTC	2 cut(s)	390, 822
AjuI	GAANNNNNNNTTGG	2 cut(s)	1422, 1454
AloI	GAACNNNNNNTCC	2 cut(s)	823, 855
Alw21I	GWGCWC	1 cut(s)	914
Alw26I	GTCTC	4 cut(s)	292, 1452, 1803, 1979
AlwI	GGATC	7 cut(s)	476, 862, 875, 1668, 1790, 1803, 1994
AlwNI	CAGNNNCTG	2 cut(s)	65, 651
AoxI	GGCC	5 cut(s)	401, 859, 991, 1852, 2377
ApoI	RAATTY	3 cut(s)	844, 1058, 2161
ArsI	GACNNNNNNTTYG	2 cut(s)	532, 564
Asp700I	GAANNNNTTC	4 cut(s)	211, 1197, 1290, 2547
AspLEI	GCGC	1 cut(s)	1366
AspS9I	GGNCC	1 cut(s)	1126
AsuHPI	GGTGA	2 cut(s)	41, 1718
AvaII	GGWCC	1 cut(s)	1126
BaeGI	GKGCMC	1 cut(s)	2301
BaeI	ACNNNNGTAYC	2 cut(s)	298, 331
BamHI	GGATCC	2 cut(s)	867, 1795
BarI	GAAGNNNNNNTAC	2 cut(s)	1307, 1339
BbsI	GAAGAC	3 cut(s)	177, 824, 2592
Bbv12I	GWGCWC	1 cut(s)	914
BbvCI	CCTCAGC	1 cut(s)	1121
BccI	CCATC	4 cut(s)	273, 547, 1414, 2388
BceAI	ACGGC	3 cut(s)	940, 1133, 2105
BclI	TGATCA	1 cut(s)	37
BcoDI	GTCTC	4 cut(s)	292, 1452, 1803, 1979
BfaI	CTAG	5 cut(s)	1335, 1580, 2168, 2447, 2573
BfmI	CTRYAG	1 cut(s)	91
BfoI	RGCGCY	1 cut(s)	1367
BfuAI	ACCTGC	3 cut(s)	121, 795, 2459
BglI	GCCNNNNNGGC	1 cut(s)	1117
Bme18I	GGWCC	1 cut(s)	1126
BmeRI	GACNNNNNGTC	1 cut(s)	2593
BmgBI	CACGTC	2 cut(s)	390, 822
BmgT120I	GGNCC	1 cut(s)	1126
BmiI	GGNNCC	3 cut(s)	869, 1127, 1797
BmrI	ACTGGG	1 cut(s)	608
BmuI	ACTGGG	1 cut(s)	608
BpiI	GAAGAC	3 cut(s)	177, 824, 2592
BpmI	CTGGAG	2 cut(s)	1413, 1476
Bpu10I	CCTNAGC	2 cut(s)	1121, 1655
BpuEI	CTTGAG	4 cut(s)	134, 1171, 1579, 1766
Bsa29I	ATCGAT	1 cut(s)	799
BsaAI	YACGTR	1 cut(s)	1530
BsaBI	GATNNNNATC	1 cut(s)	801
BsaJI	CCNNGG	6 cut(s)	234, 294, 811, 988, 1053, 2133
BsaWI	WCCGGW	1 cut(s)	2262
BsaXI	ACNNNNNCTCC	2 cut(s)	1449, 1479
Bse1I	ACTGG	5 cut(s)	268, 603, 693, 896, 1686
Bse3DI	GCAATG	3 cut(s)	411, 1596, 1941
Bse8I	GATNNNNATC	1 cut(s)	801
BseCI	ATCGAT	1 cut(s)	799
BseDI	CCNNGG	6 cut(s)	234, 294, 811, 988, 1053, 2133
BseGI	GGATG	6 cut(s)	40, 284, 779, 1378, 1942, 2380
BseJI	GATNNNNATC	1 cut(s)	801
BseMI	GCAATG	3 cut(s)	411, 1596, 1941
BseMII	CTCAG	5 cut(s)	506, 658, 894, 1112, 1655
BseNI	ACTGG	5 cut(s)	268, 603, 693, 896, 1686
BseRI	GAGGAG	1 cut(s)	2535
BseSI	GKGCMC	1 cut(s)	2301
BsgI	GTGCAG	4 cut(s)	155, 1817, 2007, 2202
BshFI	GGCC	5 cut(s)	403, 861, 993, 1854, 2379
BshVI	ATCGAT	1 cut(s)	799
BsiHKAI	GWGCWC	1 cut(s)	914
BsiSI	CCGG	3 cut(s)	1099, 1367, 2263
BslFI	GGGAC	2 cut(s)	1139, 1456
BsmAI	GTCTC	4 cut(s)	292, 1452, 1803, 1979
BsmFI	GGGAC	2 cut(s)	1139, 1456
BsmI	GAATGC	1 cut(s)	1724
BsnI	GGCC	5 cut(s)	403, 861, 993, 1854, 2379
Bsp1286I	GDGCHC	2 cut(s)	914, 2301
Bsp19I	CCATGG	3 cut(s)	294, 1053, 2133
BspACI	CCGC	5 cut(s)	444, 1163, 1721, 2010, 2525
BspANI	GGCC	5 cut(s)	403, 861, 993, 1854, 2379
BspCNI	CTCAG	5 cut(s)	505, 657, 893, 1113, 1656
BspDI	ATCGAT	1 cut(s)	799
BspHI	TCATGA	2 cut(s)	154, 505
BspLI	GGNNCC	3 cut(s)	869, 1127, 1797
BspMAI	CTGCAG	1 cut(s)	95
BspMI	ACCTGC	3 cut(s)	121, 795, 2459
BspPI	GGATC	7 cut(s)	476, 862, 875, 1668, 1790, 1803, 1994
BspQI	GCTCTTC	1 cut(s)	986
BsrBI	CCGCTC	1 cut(s)	2525
BsrDI	GCAATG	3 cut(s)	411, 1596, 1941
BsrI	ACTGG	5 cut(s)	268, 603, 693, 896, 1686
BssECI	CCNNGG	6 cut(s)	234, 294, 811, 988, 1053, 2133
BssT1I	CCWWGG	5 cut(s)	234, 294, 988, 1053, 2133
Bst6I	CTCTTC	3 cut(s)	683, 986, 1770
BstAPI	GCANNNNNTGC	2 cut(s)	65, 1186
BstBAI	YACGTR	1 cut(s)	1530
BstC8I	GCNNGC	4 cut(s)	401, 792, 1747, 2014
BstDEI	CTNAG	7 cut(s)	492, 644, 659, 880, 1121, 1655, 1664
BstDSI	CCRYGG	3 cut(s)	294, 1053, 2133
BstF5I	GGATG	6 cut(s)	40, 284, 779, 1378, 1942, 2380
BstH2I	RGCGCY	1 cut(s)	1367
BstHHI	GCGC	1 cut(s)	1366
BstMAI	GTCTC	4 cut(s)	292, 1452, 1803, 1979
BstMWI	GCNNNNNNNGC	9 cut(s)	65, 118, 127, 136, 350, 918, 1117, 1186, 2443
BstNSI	RCATGY	4 cut(s)	979, 1735, 1904, 2406
BstSFI	CTRYAG	1 cut(s)	91
BstSLI	GKGCMC	1 cut(s)	2301
BstSNI	TACGTA	1 cut(s)	1530
BstV2I	GAAGAC	3 cut(s)	177, 824, 2592
BstX2I	RGATCY	3 cut(s)	867, 1660, 1795
BstXI	CCANNNNNNTGG	1 cut(s)	275
BstYI	RGATCY	3 cut(s)	867, 1660, 1795
Bsu15I	ATCGAT	1 cut(s)	799
BsuRI	GGCC	5 cut(s)	403, 861, 993, 1854, 2379
BsuTUI	ATCGAT	1 cut(s)	799
BtgI	CCRYGG	3 cut(s)	294, 1053, 2133
BtgZI	GCGATG	1 cut(s)	1770
BtrI	CACGTC	2 cut(s)	390, 822
BtsCI	GGATG	6 cut(s)	40, 284, 779, 1378, 1942, 2380
BtsI	GCAGTG	4 cut(s)	569, 996, 1572, 2028
BtsIMutI	CAGTG	8 cut(s)	569, 596, 756, 763, 996, 1544, 1572, 2028
BveI	ACCTGC	3 cut(s)	121, 795, 2459
Cac8I	GCNNGC	4 cut(s)	401, 792, 1747, 2014
CaiI	CAGNNNCTG	2 cut(s)	65, 651
CciI	TCATGA	2 cut(s)	154, 505
CfoI	GCGC	1 cut(s)	1366
Cfr13I	GGNCC	1 cut(s)	1126
ClaI	ATCGAT	1 cut(s)	799
Csp6I	GTAC	9 cut(s)	761, 781, 1050, 1324, 1531, 1616, 1874, 1898, 2123
CviQI	GTAC	9 cut(s)	761, 781, 1050, 1324, 1531, 1616, 1874, 1898, 2123
DdeI	CTNAG	7 cut(s)	492, 644, 659, 880, 1121, 1655, 1664
DrdI	GACNNNNNNGTC	1 cut(s)	425
DriI	GACNNNNNGTC	1 cut(s)	2593
DseDI	GACNNNNNNGTC	1 cut(s)	425
Eam1104I	CTCTTC	3 cut(s)	683, 986, 1770
Eam1105I	GACNNNNNGTC	1 cut(s)	2593
EarI	CTCTTC	3 cut(s)	683, 986, 1770
EciI	GGCGGA	1 cut(s)	1178
Eco105I	TACGTA	1 cut(s)	1530
Eco130I	CCWWGG	5 cut(s)	234, 294, 988, 1053, 2133
Eco147I	AGGCCT	1 cut(s)	861
Eco32I	GATATC	2 cut(s)	700, 803
Eco47I	GGWCC	1 cut(s)	1126
Eco57I	CTGAAG	1 cut(s)	1901
EcoRI	GAATTC	1 cut(s)	1058
EcoRV	GATATC	2 cut(s)	700, 803
EcoT14I	CCWWGG	5 cut(s)	234, 294, 988, 1053, 2133
ErhI	CCWWGG	5 cut(s)	234, 294, 988, 1053, 2133
FalI	AAGNNNNNCTT	4 cut(s)	662, 694, 1248, 1280
FaqI	GGGAC	2 cut(s)	1139, 1456
FauNDI	CATATG	2 cut(s)	475, 1223
FbaI	TGATCA	1 cut(s)	37
FblI	GTMKAC	1 cut(s)	2091
FokI	GGATG	6 cut(s)	27, 291, 786, 1385, 1949, 2367
FspBI	CTAG	5 cut(s)	1335, 1580, 2168, 2447, 2573
GlaI	GCGC	1 cut(s)	1365
GsuI	CTGGAG	2 cut(s)	1413, 1476
HaeII	RGCGCY	1 cut(s)	1367
HaeIII	GGCC	5 cut(s)	403, 861, 993, 1854, 2379
HapII	CCGG	3 cut(s)	1099, 1367, 2263
HhaI	GCGC	1 cut(s)	1366
Hin6I	GCGC	1 cut(s)	1364
HinP1I	GCGC	1 cut(s)	1364
HincII	GTYRAC	3 cut(s)	943, 1737, 2092
HindII	GTYRAC	3 cut(s)	943, 1737, 2092
HindIII	AAGCTT	2 cut(s)	218, 1652
HpaI	GTTAAC	1 cut(s)	943
HpaII	CCGG	3 cut(s)	1099, 1367, 2263
HphI	GGTGA	2 cut(s)	41, 1718
Hpy166II	GTNNAC	6 cut(s)	166, 361, 763, 943, 1737, 2092
Hpy8I	GTNNAC	6 cut(s)	166, 361, 763, 943, 1737, 2092
HpyAV	CCTTC	8 cut(s)	79, 180, 236, 1031, 1309, 1697, 2226, 2510
HpyCH4IV	ACGT	7 cut(s)	162, 389, 423, 821, 1529, 1896, 2463
HpyF10VI	GCNNNNNNNGC	9 cut(s)	65, 118, 127, 136, 350, 918, 1117, 1186, 2443
HpyF3I	CTNAG	7 cut(s)	492, 644, 659, 880, 1121, 1655, 1664
HpySE526I	ACGT	7 cut(s)	162, 389, 423, 821, 1529, 1896, 2463
HspAI	GCGC	1 cut(s)	1364
Ksp22I	TGATCA	1 cut(s)	37
KspAI	GTTAAC	1 cut(s)	943
LguI	GCTCTTC	1 cut(s)	986
LmnI	GCTCC	2 cut(s)	193, 2522
MaeI	CTAG	5 cut(s)	1335, 1580, 2168, 2447, 2573
MaeII	ACGT	7 cut(s)	162, 389, 423, 821, 1529, 1896, 2463
MaeIII	GTNAC	9 cut(s)	248, 495, 745, 1474, 1548, 1892, 1919, 2188, 2506
MbiI	CCGCTC	1 cut(s)	2525
MfeI	CAATTG	1 cut(s)	1245
MflI	RGATCY	3 cut(s)	867, 1660, 1795
MhlI	GDGCHC	2 cut(s)	914, 2301
MlyI	GAGTC	4 cut(s)	503, 1677, 2087, 2528
MmeI	TCCRAC	5 cut(s)	171, 1447, 1651, 1773, 2236
MroXI	GAANNNNTTC	4 cut(s)	211, 1197, 1290, 2547
MseI	TTAA	7 cut(s)	18, 203, 384, 942, 1158, 1626, 2084
MspA1I	CMGCKG	3 cut(s)	65, 96, 2012
MspI	CCGG	3 cut(s)	1099, 1367, 2263
MunI	CAATTG	1 cut(s)	1245
Mva1269I	GAATGC	1 cut(s)	1724
MwoI	GCNNNNNNNGC	9 cut(s)	65, 118, 127, 136, 350, 918, 1117, 1186, 2443
NcoI	CCATGG	3 cut(s)	294, 1053, 2133
NdeI	CATATG	2 cut(s)	475, 1223
NlaIV	GGNNCC	3 cut(s)	869, 1127, 1797
NmeAIII	GCCGAG	1 cut(s)	329
NmuCI	GTSAC	1 cut(s)	1548
NspI	RCATGY	4 cut(s)	979, 1735, 1904, 2406
PagI	TCATGA	2 cut(s)	154, 505
PaqCI	CACCTGC	2 cut(s)	121, 2459
PceI	AGGCCT	1 cut(s)	861
PciI	ACATGT	3 cut(s)	1731, 1900, 2402
PciSI	GCTCTTC	1 cut(s)	986
PcsI	WCGNNNNNNNCGW	3 cut(s)	179, 185, 924
PctI	GAATGC	1 cut(s)	1724
PdmI	GAANNNNTTC	4 cut(s)	211, 1197, 1290, 2547
PfeI	GAWTC	8 cut(s)	31, 299, 648, 1102, 2101, 2336, 2489, 2548
PflFI	GACNNNGTC	1 cut(s)	422
PflMI	CCANNNNNTGG	2 cut(s)	1135, 2386
PleI	GAGTC	4 cut(s)	503, 1676, 2087, 2528
PpsI	GAGTC	4 cut(s)	503, 1676, 2087, 2528
Ppu21I	YACGTR	1 cut(s)	1530
PscI	ACATGT	3 cut(s)	1731, 1900, 2402
Psp1406I	AACGTT	1 cut(s)	2463
PspN4I	GGNNCC	3 cut(s)	869, 1127, 1797
PspPI	GGNCC	1 cut(s)	1126
PstI	CTGCAG	1 cut(s)	95
PstNI	CAGNNNCTG	2 cut(s)	65, 651
PsuI	RGATCY	3 cut(s)	867, 1660, 1795
PsyI	GACNNNGTC	1 cut(s)	422
PvuII	CAGCTG	2 cut(s)	65, 96
RsaI	GTAC	9 cut(s)	762, 782, 1051, 1325, 1532, 1617, 1875, 1899, 2124
RsaNI	GTAC	9 cut(s)	761, 781, 1050, 1324, 1531, 1616, 1874, 1898, 2123
SalI	GTCGAC	1 cut(s)	2090
SapI	GCTCTTC	1 cut(s)	986
SaqAI	TTAA	7 cut(s)	18, 203, 384, 942, 1158, 1626, 2084
Sau96I	GGNCC	1 cut(s)	1126
SchI	GAGTC	4 cut(s)	503, 1677, 2087, 2528
SduI	GDGCHC	2 cut(s)	914, 2301
SfcI	CTRYAG	1 cut(s)	91
SinI	GGWCC	1 cut(s)	1126
SmlI	CTYRAG	4 cut(s)	113, 1150, 1558, 1781
SmoI	CTYRAG	4 cut(s)	113, 1150, 1558, 1781
SnaBI	TACGTA	1 cut(s)	1530
SseBI	AGGCCT	1 cut(s)	861
SsiI	CCGC	5 cut(s)	444, 1163, 1721, 2010, 2525
SspI	AATATT	1 cut(s)	1134
SspMI	CTAG	5 cut(s)	1335, 1580, 2168, 2447, 2573
StuI	AGGCCT	1 cut(s)	861
StyI	CCWWGG	5 cut(s)	234, 294, 988, 1053, 2133
TaiI	ACGT	7 cut(s)	165, 392, 426, 824, 1532, 1899, 2466
TaqI	TCGA	6 cut(s)	245, 429, 799, 951, 2091, 2551
TatI	WGTACW	4 cut(s)	760, 780, 1323, 1615
TauI	GCSGC	2 cut(s)	446, 1724
TfiI	GAWTC	8 cut(s)	31, 299, 648, 1102, 2101, 2336, 2489, 2548
Tru1I	TTAA	7 cut(s)	18, 203, 384, 942, 1158, 1626, 2084
Tru9I	TTAA	7 cut(s)	18, 203, 384, 942, 1158, 1626, 2084
TscAI	CASTG	8 cut(s)	576, 603, 756, 770, 1003, 1551, 1579, 2035
TseFI	GTSAC	1 cut(s)	1548
Tsp45I	GTSAC	1 cut(s)	1548
TspGWI	ACGGA	1 cut(s)	33
TspRI	CASTG	8 cut(s)	576, 603, 756, 770, 1003, 1551, 1579, 2035
Tth111I	GACNNNGTC	1 cut(s)	422
Van91I	CCANNNNNTGG	2 cut(s)	1135, 2386
VpaK11BI	GGWCC	1 cut(s)	1126
XapI	RAATTY	3 cut(s)	844, 1058, 2161
XceI	RCATGY	4 cut(s)	979, 1735, 1904, 2406
XmiI	GTMKAC	1 cut(s)	2091
XmnI	GAANNNNTTC	4 cut(s)	211, 1197, 1290, 2547
XspI	CTAG	5 cut(s)	1335, 1580, 2168, 2447, 2573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.