AT4G22270

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007624
Physical Location & Seq
Forward (+)
11773221 .. 11776039
2819 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22270.2

Sequence Viewer

Length: 1314 bp
ATGGCGACGAACGGATCGTCGGAGCATCTTCTCCCGGAGACGACAAGACCAAGCGCGATCATCAACAAACAACAACCGGAGTTTCCTGCATCACGATTCACGTTCATGTCCCTCGTGCTCTGGTTCGACCAGTCAAATTTCGGTACGGCTCTGCTTTCGTGGTCCGTTTTCTTCCTTCTCGTCGTCATCGTTCCTTTGATCTCTCACTTCTTGCTCGTTTGCTCCGACTGCGATTTCCATCACCGACGTCCGTACGACGTTATTGTGCAGCTTTCGTTATCGATTTTCGCAGGAATCTCGTTCGTTAGCTTATCGATTTGGTCGAGAAAATTTGGGATGCGACGGTTTTTGTTCCTTGATAAGCTTTGGGATGTTAGCGACAAAGTTAGAATCGAATACGAAGCTGAAATTCAGAGATCACTAAAACGGCTAATGATCTTCGTCCTCCCATCACTAACACTCGAAGCAACCTACAGAATCTGGTGGTACATCTCTGGCTTCAACCAGATTCCTTACATCATCAATCCTATTCTTAGCCACGTCGTCGCTTGCACTCTCCAGCTCTCTTCTTGGCTTTACCGTAACTCCTTATTCATCATTGTCTGCATTCTCTACAAAATCACATGTCATCTACAAACTCTTCGTCTTGATGACTTCGCTCGTTGCTTCGCTTCTGAGATCACTGACGTTAGATCAGCTCTCGGCGAGCATCAAAAGATTCGTCGTAATTTGAGGATTGTTAGTCATCGATTCAGGAGATTTATTCTTTTGTCTTTGATTCTTGTTACTGCTACTCAGTTCATGGCTTTGCTTACTACTACGAGAGCTAGTGTTGCTGTTAATATCTATGAAGTTGGCGAACTCGCGTTATGTTCGTTGAGTTTGGTTACAGGAGTATTCATATGTTTGAGAAGTGCAACGAAGATAACTCACAAAGCTCAATCTGTAACCAGCCTTGCAGCTAAATGGAACGTTTGCGCAACAGTTGACTCGTTTGATCATCTTGATGGCGAAACTCCTACTGGTTCCATTATTGAATCTCAAGTTTCCCTACGCGGCAATGCGATAGAGACGTCTGATGATGAAGAAGGAGAAGGAGACGATGATCTCGATAATACCAAGATACATCCCATCTATGCCAATACAATTTCTTACCAAAAACGTCAAGCTCTAGTGACGTATCTAGAGAACAACAAAGCTGGGATCACTGTGTATGGATTCCTTGTAGATAGATCATGGTTGAATACGATTTTCGGGATTGAGCTCGCTCTTCTACTATGGTTGCTCAATAAAACAATCGTGAATATACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

437

Amino Acids

49.79

Weight (kDa)

7.61

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 38 - 420 1.4e-158 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 250, 1076
Acc16I TGCGCA 1 cut(s) 979
AccII CGCG 3 cut(s) 56, 866, 1056
AciI CCGC 1 cut(s) 1056
AclI AACGTT 1 cut(s) 972
AclWI GGATC 2 cut(s) 22, 1211
AcsI RAATTY 3 cut(s) 136, 329, 408
AcyI GRCGYC 2 cut(s) 247, 1073
AfaI GTAC 3 cut(s) 145, 254, 488
AflIII ACRYGT 1 cut(s) 623
AgsI TTSAA 3 cut(s) 502, 1037, 1243
AjiI CACGTC 1 cut(s) 541
Alw21I GWGCWC 2 cut(s) 120, 1266
Alw26I GTCTC 3 cut(s) 32, 1064, 1092
AlwI GGATC 2 cut(s) 22, 1211
AlwNI CAGNNNCTG 1 cut(s) 480
ApeKI GCWGC 2 cut(s) 268, 959
ApoI RAATTY 3 cut(s) 136, 329, 408
ArsI GACNNNNNNTTYG 5 cut(s) 34, 628, 660, 706, 738
AspLEI GCGC 2 cut(s) 56, 980
AspS9I GGNCC 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 162
BanII GRGCYC 1 cut(s) 1266
BauI CACGAG 1 cut(s) 113
Bbv12I GWGCWC 2 cut(s) 120, 1266
BbvI GCAGC 2 cut(s) 280, 971
BccI CCATC 4 cut(s) 246, 457, 1001, 1139
BceAI ACGGC 2 cut(s) 162, 443
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BclI TGATCA 1 cut(s) 997
BcnI CCSGG 1 cut(s) 35
BcoDI GTCTC 3 cut(s) 32, 1064, 1092
BfaI CTAG 3 cut(s) 828, 1172, 1184
BfmI CTRYAG 1 cut(s) 472
BisI GCNGC 3 cut(s) 269, 960, 1057
BlsI GCNGC 3 cut(s) 270, 961, 1058
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 162
BmgBI CACGTC 1 cut(s) 541
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 1027
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 4 cut(s) 34, 98, 327, 718
BpmI CTGGAG 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 1026
BpuMI CCSGG 1 cut(s) 35
Bsa29I ATCGAT 3 cut(s) 281, 314, 748
BsaBI GATNNNNATC 1 cut(s) 237
BsaHI GRCGYC 2 cut(s) 247, 1073
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 569, 599
Bse1I ACTGG 2 cut(s) 130, 1027
Bse3DI GCAATG 1 cut(s) 1066
Bse8I GATNNNNATC 1 cut(s) 237
BseCI ATCGAT 3 cut(s) 281, 314, 748
BseGI GGATG 3 cut(s) 342, 376, 1126
BseJI GATNNNNATC 1 cut(s) 237
BseMI GCAATG 1 cut(s) 1066
BseMII CTCAG 2 cut(s) 666, 809
BseNI ACTGG 2 cut(s) 130, 1027
BseXI GCAGC 2 cut(s) 280, 971
BseYI CCCAGC 1 cut(s) 1199
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 3 cut(s) 56, 866, 1056
BshVI ATCGAT 3 cut(s) 281, 314, 748
BsiHKAI GWGCWC 2 cut(s) 120, 1266
BsiSI CCGG 2 cut(s) 35, 77
BsiWI CGTACG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 94
BsmAI GTCTC 3 cut(s) 32, 1064, 1092
BsmBI CGTCTC 3 cut(s) 32, 1064, 1092
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 606
Bsp1286I GDGCHC 2 cut(s) 120, 1266
BspACI CCGC 1 cut(s) 1056
BspCNI CTCAG 2 cut(s) 667, 808
BspDI ATCGAT 3 cut(s) 281, 314, 748
BspFNI CGCG 3 cut(s) 56, 866, 1056
BspLI GGNNCC 1 cut(s) 1027
BspPI GGATC 2 cut(s) 22, 1211
BspQI GCTCTTC 1 cut(s) 1275
BsrDI GCAATG 1 cut(s) 1066
BsrI ACTGG 2 cut(s) 130, 1027
BssNI GRCGYC 2 cut(s) 247, 1073
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 4 cut(s) 345, 581, 985, 1210
Bst6I CTCTTC 3 cut(s) 571, 645, 1275
BstACI GRCGYC 2 cut(s) 247, 1073
BstC8I GCNNGC 3 cut(s) 550, 707, 1266
BstDEI CTNAG 3 cut(s) 533, 675, 795
BstF5I GGATG 3 cut(s) 342, 376, 1126
BstFNI CGCG 3 cut(s) 56, 866, 1056
BstHHI GCGC 2 cut(s) 56, 980
BstMAI GTCTC 3 cut(s) 32, 1064, 1092
BstMWI GCNNNNNNNGC 2 cut(s) 228, 833
BstNSI RCATGY 1 cut(s) 627
BstSCI CCNGG 1 cut(s) 33
BstSFI CTRYAG 1 cut(s) 472
BstUI CGCG 3 cut(s) 56, 866, 1056
BstV1I GCAGC 2 cut(s) 280, 971
Bsu15I ATCGAT 3 cut(s) 281, 314, 748
BsuTUI ATCGAT 3 cut(s) 281, 314, 748
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 3 cut(s) 342, 376, 1126
BtsIMutI CAGTG 2 cut(s) 681, 1206
Cac8I GCNNGC 3 cut(s) 550, 707, 1266
CaiI CAGNNNCTG 1 cut(s) 480
CfoI GCGC 2 cut(s) 56, 980
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 3 cut(s) 281, 314, 748
Csp6I GTAC 3 cut(s) 144, 253, 487
CviAII CATG 5 cut(s) 106, 624, 802, 1236, 1311
CviQI GTAC 3 cut(s) 144, 253, 487
DdeI CTNAG 3 cut(s) 533, 675, 795
Eam1104I CTCTTC 3 cut(s) 571, 645, 1275
EarI CTCTTC 3 cut(s) 571, 645, 1275
Ecl136II GAGCTC 1 cut(s) 1264
Eco24I GRGCYC 1 cut(s) 1266
Eco47I GGWCC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 1264
EcoICRI GAGCTC 1 cut(s) 1264
EcoT38I GRGCYC 1 cut(s) 1266
Esp3I CGTCTC 3 cut(s) 32, 1064, 1092
FaeI CATG 5 cut(s) 109, 627, 805, 1239, 1314
FaqI GGGAC 1 cut(s) 94
FatI CATG 5 cut(s) 105, 623, 801, 1235, 1310
FauNDI CATATG 1 cut(s) 902
FbaI TGATCA 1 cut(s) 997
Fnu4HI GCNGC 3 cut(s) 269, 960, 1057
FokI GGATG 3 cut(s) 349, 383, 1113
FriOI GRGCYC 1 cut(s) 1266
Fsp4HI GCNGC 3 cut(s) 269, 960, 1057
FspBI CTAG 3 cut(s) 828, 1172, 1184
FspI TGCGCA 1 cut(s) 979
GlaI GCGC 2 cut(s) 55, 979
GluI GCNGC 3 cut(s) 269, 960, 1057
GsaI CCCAGC 1 cut(s) 1203
GsuI CTGGAG 1 cut(s) 542
HapII CCGG 2 cut(s) 35, 77
HhaI GCGC 2 cut(s) 56, 980
Hin1I GRCGYC 2 cut(s) 247, 1073
Hin1II CATG 5 cut(s) 109, 627, 805, 1239, 1314
Hin6I GCGC 2 cut(s) 54, 978
HinP1I GCGC 2 cut(s) 54, 978
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HindIII AAGCTT 1 cut(s) 362
HpaII CCGG 2 cut(s) 35, 77
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 988
Hpy188I TCNGA 5 cut(s) 22, 226, 414, 676, 1078
Hpy188III TCNNGA 9 cut(s) 93, 324, 647, 754, 1004, 1109, 1184, 1255, 1300
Hpy8I GTNNAC 1 cut(s) 988
Hpy99I CGWCG 9 cut(s) 10, 22, 185, 249, 260, 345, 545, 548, 726
HpyAV CCTTC 3 cut(s) 185, 1082, 1088
HpyCH4III ACNGT 4 cut(s) 345, 581, 985, 1210
HpyCH4IV ACGT 9 cut(s) 101, 247, 258, 540, 687, 972, 1073, 1162, 1178
HpyCH4V TGCA 6 cut(s) 89, 268, 552, 606, 917, 959
HpyF10VI GCNNNNNNNGC 2 cut(s) 228, 833
HpyF3I CTNAG 3 cut(s) 533, 675, 795
HpySE526I ACGT 9 cut(s) 101, 247, 258, 540, 687, 972, 1073, 1162, 1178
Hsp92I GRCGYC 2 cut(s) 247, 1073
Hsp92II CATG 5 cut(s) 109, 627, 805, 1239, 1314
HspAI GCGC 2 cut(s) 54, 978
Ksp22I TGATCA 1 cut(s) 997
LguI GCTCTTC 1 cut(s) 1275
LmnI GCTCC 2 cut(s) 22, 227
Lsp1109I GCAGC 2 cut(s) 280, 971
LweI GCATC 4 cut(s) 34, 98, 327, 718
MaeI CTAG 3 cut(s) 828, 1172, 1184
MaeII ACGT 9 cut(s) 101, 247, 258, 540, 687, 972, 1073, 1162, 1178
MaeIII GTNAC 5 cut(s) 581, 784, 886, 946, 1174
MboII GAAGA 8 cut(s) 20, 163, 430, 558, 632, 934, 1097, 1262
MhlI GDGCHC 2 cut(s) 120, 1266
MluCI AATT 5 cut(s) 136, 329, 408, 727, 1146
MlyI GAGTC 1 cut(s) 983
MmeI TCCRAC 1 cut(s) 249
MnlI CCTC 3 cut(s) 122, 455, 726
MseI TTAA 1 cut(s) 840
MslI CAYNNNNRTG 2 cut(s) 104, 1005
MspI CCGG 2 cut(s) 35, 77
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 606
MvnI CGCG 3 cut(s) 56, 866, 1056
MwoI GCNNNNNNNGC 2 cut(s) 228, 833
NciI CCSGG 1 cut(s) 35
NdeI CATATG 1 cut(s) 902
NlaIII CATG 5 cut(s) 109, 627, 805, 1239, 1314
NlaIV GGNNCC 1 cut(s) 1027
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 1 cut(s) 1174
NsbI TGCGCA 1 cut(s) 979
NspI RCATGY 1 cut(s) 627
PciI ACATGT 1 cut(s) 623
PciSI GCTCTTC 1 cut(s) 1275
PcsI WCGNNNNNNNCGW 5 cut(s) 14, 186, 222, 320, 1107
PctI GAATGC 1 cut(s) 606
Pfl23II CGTACG 1 cut(s) 252
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 3 cut(s) 270, 961, 1058
PleI GAGTC 1 cut(s) 983
PpsI GAGTC 1 cut(s) 983
PscI ACATGT 1 cut(s) 623
Psp124BI GAGCTC 1 cut(s) 1266
Psp1406I AACGTT 1 cut(s) 972
PspFI CCCAGC 1 cut(s) 1199
PspLI CGTACG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 1027
PspPI GGNCC 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 480
RsaI GTAC 3 cut(s) 145, 254, 488
RsaNI GTAC 3 cut(s) 144, 253, 487
RseI CAYNNNNRTG 2 cut(s) 104, 1005
SacI GAGCTC 1 cut(s) 1266
SapI GCTCTTC 1 cut(s) 1275
SaqAI TTAA 1 cut(s) 840
SatI GCNGC 3 cut(s) 269, 960, 1057
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 1 cut(s) 983
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 2 cut(s) 120, 1266
SfaNI GCATC 4 cut(s) 34, 98, 327, 718
SfcI CTRYAG 1 cut(s) 472
SinI GGWCC 1 cut(s) 162
SmiMI CAYNNNNRTG 2 cut(s) 104, 1005
SmlI CTYRAG 1 cut(s) 1041
SmoI CTYRAG 1 cut(s) 1041
Sse9I AATT 5 cut(s) 136, 329, 408, 727, 1146
SsiI CCGC 1 cut(s) 1056
SspMI CTAG 3 cut(s) 828, 1172, 1184
SstI GAGCTC 1 cut(s) 1266
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 4 cut(s) 345, 581, 985, 1210
TaiI ACGT 9 cut(s) 104, 250, 261, 543, 690, 975, 1076, 1165, 1181
TaqI TCGA 8 cut(s) 126, 281, 314, 323, 393, 462, 748, 1110
TasI AATT 5 cut(s) 136, 329, 408, 727, 1146
TauI GCSGC 1 cut(s) 1059
Tru1I TTAA 1 cut(s) 840
Tru9I TTAA 1 cut(s) 840
TscAI CASTG 2 cut(s) 688, 1213
TseFI GTSAC 1 cut(s) 1174
TseI GCWGC 2 cut(s) 268, 959
Tsp45I GTSAC 1 cut(s) 1174
TspDTI ATGAA 6 cut(s) 94, 583, 790, 864, 889, 1098
TspGWI ACGGA 3 cut(s) 27, 154, 240
TspRI CASTG 2 cut(s) 688, 1213
VpaK11BI GGWCC 1 cut(s) 162
XapI RAATTY 3 cut(s) 136, 329, 408
XbaI TCTAGA 1 cut(s) 1183
XceI RCATGY 1 cut(s) 627
XspI CTAG 3 cut(s) 828, 1172, 1184
ZraI GACGTC 2 cut(s) 248, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.