MD10G1328800.v1.1

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007624
Physical Location & Seq
Forward (+)
40780525 .. 40782197
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1328800.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGGAAGAACAGATGATAGCAAAGCAGAAAGCCAATAGCTCAGACTACTTAGGCCGCGAAGGAAGTGAATTCAAGAGCTTCGGAGTATGCCTCAAATGGGTGTTCGTGGATCAGTCCAGCATATGGAGGACCGGACTTTCATGGTCCGTCTTCTTCGTCTTAGCCATTGGTGTACCACTCGCGTCTCACTTTCTCCTCGCCTGCTCCGACTGCGATACCAATCACAGCCGCCCTTACCATGTTGTCTCCCAACTTTCGCTTTCCTTGTTCTCGGTGCTCGCATTCGTCAGCCTCTCTATCTGGACTCACAGATACGGTTTAAGAAAGTTTCTCTTCCTTGACAGGTTATATGAATCAAGCGAGAAAGTTCGCCATGGATATAAACAACAGTTCCAGCTAAAGTTATTGAAACTTCGATCTCTAATCTCGAACTTGGGATTGCAGAATTCAATGAAGCTCCTTTGCTTGATCGTCATCCCCTGTTTTGTGTTAGAGTGTGTCTACAAAATATGGTGGTACGTCTCGGGAGCAAGAGAATTGCCGTACTACGGCAACGTCTATTTAAGCAACACCATTTTGTGCATGTTGGAGCTGATTTCATGGCTCTATCGAACCACGATTTTCTTCCTCGTTTGTGTTCTGTTCCGGCTCGTTTGCCATCTTCAAATACTTAGGCTACAAAATTTTGCACAAGTTTTCCAAGAGGAGACCGAGGTCGAGTTGATCTTGATGGAACACCTCAAGATCAGACGGAACCTCCGGTCGATGAGCCATCGGTTTCGCATATTCATCTTGTTTTCTCTGATCATGGTTACAGCAAGTCAGCTCACTTCTCTGGTGTTGGTAACAAGGTCTAGTGCTCATGTCAACATCTACAAGGCCGGGGAGCTTGCGTTATGTTCTATTAGCCTAGTGACTAGCCTTTTCATTTGCTTGAGAAGTGCAACCAAAATAACTCACAAGGCACAGTCAATTACAGGCCTTGCTACAAAGTGGCATGTGTGCGCCACATTACACTCTTTTGATTACACGGAAACTGAGACTCCGATGGCTCCGATTTCTTCAACTCCGGCGTTTCCTTTCGGTGTTGACATGGAATCGGAAGATGAAGAAGGTGTAGAAGATGACGATTTGGACAGCACGAAGTTAGTACCAATTTATAGTCAAACAATCTCGTTCCAGAAAAGACAGGCTCTAGTGACATATTTGGAGAACAATAGAGCAGGGATAACAGTTTTCGGGTTCACGGTGGATCGAATGTGGCTTCACTCCATTTTTGCAATTCAACTGGCTCTTCTGCTATGGCTGCTGAACAAGACGCTGGGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

444

Amino Acids

50.87

Weight (kDa)

8.52

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 23 - 428 1.5e-157 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 123
AccI GTMKAC 1 cut(s) 501
AccII CGCG 2 cut(s) 57, 182
AciI CCGC 2 cut(s) 55, 229
AclWI GGATC 2 cut(s) 117, 1260
AcsI RAATTY 3 cut(s) 68, 445, 682
AfaI GTAC 4 cut(s) 174, 518, 545, 1152
AfiI CCNNNNNNNGG 3 cut(s) 97, 123, 548
AgsI TTSAA 6 cut(s) 73, 409, 450, 665, 1065, 1286
AluBI AGCT 7 cut(s) 39, 78, 397, 457, 592, 826, 889
AluI AGCT 7 cut(s) 39, 78, 397, 457, 592, 826, 889
Alw21I GWGCWC 2 cut(s) 279, 862
Alw26I GTCTC 5 cut(s) 189, 250, 526, 701, 1034
AlwI GGATC 2 cut(s) 117, 1260
Ama87I CYCGRG 1 cut(s) 523
AoxI GGCC 3 cut(s) 52, 879, 979
ApeKI GCWGC 1 cut(s) 1306
ApoI RAATTY 3 cut(s) 68, 445, 682
AspLEI GCGC 1 cut(s) 1007
AspS9I GGNCC 2 cut(s) 129, 144
AsuC2I CCSGG 1 cut(s) 883
AvaI CYCGRG 1 cut(s) 523
AvaII GGWCC 2 cut(s) 129, 144
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 2 cut(s) 279, 862
BbvI GCAGC 1 cut(s) 1293
BccI CCATC 4 cut(s) 666, 724, 780, 1042
BceAI ACGGC 2 cut(s) 526, 565
BclI TGATCA 1 cut(s) 804
BcnI CCSGG 1 cut(s) 883
BcoDI GTCTC 5 cut(s) 189, 250, 526, 701, 1034
BfaI CTAG 4 cut(s) 855, 911, 918, 1196
BisI GCNGC 3 cut(s) 55, 229, 1307
BlsI GCNGC 3 cut(s) 56, 230, 1308
Bme1390I CCNGG 1 cut(s) 883
Bme18I GGWCC 2 cut(s) 129, 144
BmeT110I CYCGRG 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 129, 144
BmiI GGNNCC 2 cut(s) 755, 1053
BmrFI CCNGG 1 cut(s) 883
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 1 cut(s) 142
BplI GAGNNNNNCTC 2 cut(s) 75, 107
BpuEI CTTGAG 2 cut(s) 725, 955
BpuMI CCSGG 1 cut(s) 883
BsaBI GATNNNNATC 2 cut(s) 219, 473
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 3 cut(s) 373, 711, 882
BsaWI WCCGGW 2 cut(s) 131, 759
BsaXI ACNNNNNCTCC 6 cut(s) 741, 771, 878, 908, 1027, 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 123, 548
Bse1I ACTGG 1 cut(s) 1293
Bse8I GATNNNNATC 2 cut(s) 219, 473
BseDI CCNNGG 3 cut(s) 373, 711, 882
BseGI GGATG 1 cut(s) 474
BseJI GATNNNNATC 2 cut(s) 219, 473
BseLI CCNNNNNNNGG 3 cut(s) 97, 123, 548
BseMII CTCAG 2 cut(s) 54, 1029
BseNI ACTGG 1 cut(s) 1293
BseRI GAGGAG 2 cut(s) 185, 719
BseXI GCAGC 1 cut(s) 1293
BseYI CCCAGC 1 cut(s) 1321
Bsh1236I CGCG 2 cut(s) 57, 182
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 3 cut(s) 54, 881, 981
BsiEI CGRYCG 1 cut(s) 764
BsiHKAI GWGCWC 2 cut(s) 279, 862
BsiHKCI CYCGRG 1 cut(s) 523
BsiSI CCGG 5 cut(s) 132, 646, 760, 882, 1070
BslI CCNNNNNNNGG 3 cut(s) 97, 123, 548
BsmAI GTCTC 5 cut(s) 189, 250, 526, 701, 1034
BsmBI CGTCTC 2 cut(s) 189, 526
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 3 cut(s) 54, 881, 981
Bso31I GGTCTC 1 cut(s) 701
BsoBI CYCGRG 1 cut(s) 523
Bsp1286I GDGCHC 2 cut(s) 279, 862
Bsp143I GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 2 cut(s) 55, 229
BspANI GGCC 3 cut(s) 54, 881, 981
BspCNI CTCAG 2 cut(s) 53, 1030
BspFNI CGCG 2 cut(s) 57, 182
BspLI GGNNCC 2 cut(s) 755, 1053
BspPI GGATC 2 cut(s) 117, 1260
BspQI GCTCTTC 1 cut(s) 1299
BspTNI GGTCTC 1 cut(s) 701
BsrI ACTGG 1 cut(s) 1293
BssECI CCNNGG 3 cut(s) 373, 711, 882
BssMI GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
BssT1I CCWWGG 1 cut(s) 373
Bst4CI ACNGT 5 cut(s) 317, 390, 969, 1234, 1249
Bst6I CTCTTC 2 cut(s) 338, 1299
BstC8I GCNNGC 3 cut(s) 202, 279, 891
BstDEI CTNAG 5 cut(s) 40, 49, 160, 671, 1038
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 1 cut(s) 474
BstFNI CGCG 2 cut(s) 57, 182
BstHHI GCGC 1 cut(s) 1007
BstKTI GATC 7 cut(s) 112, 419, 471, 726, 747, 807, 1255
BstMAI GTCTC 5 cut(s) 189, 250, 526, 701, 1034
BstMBI GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 2 cut(s) 210, 1306
BstNSI RCATGY 2 cut(s) 586, 1001
BstPAI GACNNNNGTC 1 cut(s) 713
BstSCI CCNGG 1 cut(s) 881
BstUI CGCG 2 cut(s) 57, 182
BstV1I GCAGC 1 cut(s) 1293
BstV2I GAAGAC 1 cut(s) 142
BsuRI GGCC 3 cut(s) 54, 881, 981
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 1 cut(s) 474
Cac8I GCNNGC 3 cut(s) 202, 279, 891
CfoI GCGC 1 cut(s) 1007
Cfr13I GGNCC 2 cut(s) 129, 144
CseI GACGC 2 cut(s) 171, 1327
Csp6I GTAC 4 cut(s) 173, 517, 544, 1151
CviAII CATG 9 cut(s) 141, 239, 374, 583, 600, 808, 863, 998, 1093
CviQI GTAC 4 cut(s) 173, 517, 544, 1151
DdeI CTNAG 5 cut(s) 40, 49, 160, 671, 1038
DpnI GATC 7 cut(s) 111, 418, 470, 725, 746, 806, 1254
DpnII GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
Eam1104I CTCTTC 2 cut(s) 338, 1299
EarI CTCTTC 2 cut(s) 338, 1299
Eco130I CCWWGG 1 cut(s) 373
Eco147I AGGCCT 1 cut(s) 981
Eco31I GGTCTC 1 cut(s) 701
Eco47I GGWCC 2 cut(s) 129, 144
Eco88I CYCGRG 1 cut(s) 523
EcoRI GAATTC 2 cut(s) 68, 445
EcoT14I CCWWGG 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 373
Esp3I CGTCTC 2 cut(s) 189, 526
FaeI CATG 9 cut(s) 144, 242, 377, 586, 603, 811, 866, 1001, 1096
FalI AAGNNNNNCTT 2 cut(s) 317, 349
FatI CATG 9 cut(s) 140, 238, 373, 582, 599, 807, 862, 997, 1092
FauNDI CATATG 1 cut(s) 122
FbaI TGATCA 1 cut(s) 804
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 55, 229, 1307
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 55, 229, 1307
FspBI CTAG 4 cut(s) 855, 911, 918, 1196
GlaI GCGC 1 cut(s) 1006
GluI GCNGC 3 cut(s) 55, 229, 1307
GsaI CCCAGC 1 cut(s) 1325
HaeIII GGCC 3 cut(s) 54, 881, 981
HapII CCGG 5 cut(s) 132, 646, 760, 882, 1070
HgaI GACGC 2 cut(s) 171, 1327
HhaI GCGC 1 cut(s) 1007
Hin1II CATG 9 cut(s) 144, 242, 377, 586, 603, 811, 866, 1001, 1096
Hin6I GCGC 1 cut(s) 1005
HinP1I GCGC 1 cut(s) 1005
HincII GTYRAC 2 cut(s) 868, 1090
HindII GTYRAC 2 cut(s) 868, 1090
HinfI GANTC 4 cut(s) 304, 353, 1042, 1097
HpaII CCGG 5 cut(s) 132, 646, 760, 882, 1070
Hpy166II GTNNAC 5 cut(s) 173, 502, 868, 1090, 1245
Hpy188I TCNGA 8 cut(s) 43, 83, 208, 749, 804, 1047, 1056, 1102
Hpy188III TCNNGA 7 cut(s) 73, 301, 427, 525, 727, 742, 1180
Hpy8I GTNNAC 5 cut(s) 173, 502, 868, 1090, 1245
HpyAV CCTTC 2 cut(s) 53, 1106
HpyCH4III ACNGT 5 cut(s) 317, 390, 969, 1234, 1249
HpyCH4IV ACGT 2 cut(s) 519, 555
HpyCH4V TGCA 5 cut(s) 442, 582, 689, 944, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 210, 1306
HpyF3I CTNAG 5 cut(s) 40, 49, 160, 671, 1038
HpySE526I ACGT 2 cut(s) 519, 555
Hsp92II CATG 9 cut(s) 144, 242, 377, 586, 603, 811, 866, 1001, 1096
HspAI GCGC 1 cut(s) 1005
Ksp22I TGATCA 1 cut(s) 804
Kzo9I GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
LguI GCTCTTC 1 cut(s) 1299
LmnI GCTCC 6 cut(s) 209, 462, 527, 589, 886, 1057
Lsp1109I GCAGC 1 cut(s) 1293
MaeI CTAG 4 cut(s) 855, 911, 918, 1196
MaeII ACGT 2 cut(s) 519, 555
MaeIII GTNAC 4 cut(s) 811, 844, 913, 1198
MalI GATC 7 cut(s) 111, 418, 470, 725, 746, 806, 1254
MboI GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
MhlI GDGCHC 2 cut(s) 279, 862
MluCI AATT 7 cut(s) 68, 445, 536, 682, 972, 1155, 1281
MlyI GAGTC 2 cut(s) 298, 1036
MmeI TCCRAC 2 cut(s) 231, 567
MnlI CCTC 9 cut(s) 101, 120, 206, 302, 638, 697, 706, 749, 767
MseI TTAA 2 cut(s) 320, 563
MspI CCGG 5 cut(s) 132, 646, 760, 882, 1070
MspR9I CCNGG 1 cut(s) 883
Mva1269I GAATGC 1 cut(s) 281
MvnI CGCG 2 cut(s) 57, 182
MwoI GCNNNNNNNGC 2 cut(s) 210, 1306
NciI CCSGG 1 cut(s) 883
NcoI CCATGG 1 cut(s) 373
NdeI CATATG 1 cut(s) 122
NdeII GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
NlaIII CATG 9 cut(s) 144, 242, 377, 586, 603, 811, 866, 1001, 1096
NlaIV GGNNCC 2 cut(s) 755, 1053
NmuCI GTSAC 2 cut(s) 913, 1198
NspI RCATGY 2 cut(s) 586, 1001
PceI AGGCCT 1 cut(s) 981
PciSI GCTCTTC 1 cut(s) 1299
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 353, 1097
PflMI CCANNNNNTGG 1 cut(s) 123
PkrI GCNGC 3 cut(s) 56, 230, 1308
PleI GAGTC 2 cut(s) 298, 1036
PpsI GAGTC 2 cut(s) 298, 1036
PshAI GACNNNNGTC 1 cut(s) 713
PspFI CCCAGC 1 cut(s) 1321
PspN4I GGNNCC 2 cut(s) 755, 1053
PspPI GGNCC 2 cut(s) 129, 144
RsaI GTAC 4 cut(s) 174, 518, 545, 1152
RsaNI GTAC 4 cut(s) 173, 517, 544, 1151
SapI GCTCTTC 1 cut(s) 1299
SaqAI TTAA 2 cut(s) 320, 563
SatI GCNGC 3 cut(s) 55, 229, 1307
Sau3AI GATC 7 cut(s) 109, 416, 468, 723, 744, 804, 1252
Sau96I GGNCC 2 cut(s) 129, 144
SchI GAGTC 2 cut(s) 298, 1036
ScrFI CCNGG 1 cut(s) 883
SduI GDGCHC 2 cut(s) 279, 862
SinI GGWCC 2 cut(s) 129, 144
SmlI CTYRAG 2 cut(s) 740, 934
SmoI CTYRAG 2 cut(s) 740, 934
Sse9I AATT 7 cut(s) 68, 445, 536, 682, 972, 1155, 1281
SseBI AGGCCT 1 cut(s) 981
SsiI CCGC 2 cut(s) 55, 229
SspMI CTAG 4 cut(s) 855, 911, 918, 1196
StuI AGGCCT 1 cut(s) 981
StyD4I CCNGG 1 cut(s) 881
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 5 cut(s) 317, 390, 969, 1234, 1249
TaiI ACGT 2 cut(s) 522, 558
TaqI TCGA 6 cut(s) 415, 428, 610, 717, 764, 1255
TaqII GACCGA 1 cut(s) 725
TasI AATT 7 cut(s) 68, 445, 536, 682, 972, 1155, 1281
TauI GCSGC 2 cut(s) 57, 231
TfiI GAWTC 2 cut(s) 353, 1097
Tru1I TTAA 2 cut(s) 320, 563
Tru9I TTAA 2 cut(s) 320, 563
TseFI GTSAC 2 cut(s) 913, 1198
TseI GCWGC 1 cut(s) 1306
Tsp45I GTSAC 2 cut(s) 913, 1198
TspDTI ATGAA 7 cut(s) 129, 366, 467, 588, 778, 916, 1122
TspGWI ACGGA 3 cut(s) 136, 766, 1046
Van91I CCANNNNNTGG 1 cut(s) 123
VpaK11BI GGWCC 2 cut(s) 129, 144
XapI RAATTY 3 cut(s) 68, 445, 682
XceI RCATGY 2 cut(s) 586, 1001
XmiI GTMKAC 1 cut(s) 501
XspI CTAG 4 cut(s) 855, 911, 918, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.