AT4G22320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013927
Physical Location & Seq
Reverse (-)
11792813 .. 11794778
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22320.2

Sequence Viewer

Length: 717 bp
ATGGAAGGAGTTGGTGCACGGTTAGGTAGGTCCTCGACACGGTACGGACCGGCAACGGTTTTCACCGGACCGGTGAGGAAGTGGAAGAAGAAGTGGGTACACGTCTCTCCTTCCTCTAAGAAAGACAATAATAATAGCTCCTCCGGTTCCGCCGCTGCTGCAGCTTCCGTCGTTAACGGTGGTTCGAATTCTGACGGTAGTAATGGATCGCATTTGTTGCTGTATAAGTGGGCACCATTGTCTCAGAATGGTAACGGCAATGAAGATGGTAAAAGTGAGAGTAATTCTCCGAGCGAGGATACGGTGGCAACGGTGGCAGAAGATCCTCCACGGCGGAGATTCAAATACGTTCCGATAGCAGTACTTGAGGAACAGAAGAAGGAAATTACAGAAATTGAGGAAGATGATAAGATTGAGGAGGATGACAAGATTGATGAGGATAATAAGGTTGAGCAGGAAGACAAGGTTGATGAGGACAAAACTGTAGAGGAGTCGAGCGAGAAGAAAGCGGAAGTGGAAGTGGAGGAAAAGCCTGACATCAATGATGTTCCGATGGAAGATATTCAGCAGGTTGAAGAAAAAATAGTACAGGATGATGAAGAAAAAGTAGTGCGACAAGATTTGAACGAAAGCACTGTGGATTTAGGACTGAACTTAAATGCAAACGATGCTGATGCAGAAAACGACCCGAAAGAGGACAAGCCATTAGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

26.28

Weight (kDa)

4.41

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22320 AT4G22320
fragaria_vesca FvH4_3g01090
malus_domestica MD05G1356800.v1.1 MD10G1329900.v1.1
prunus_persica Prupe.4G012100_v2.0.a1
pyrus_communis pycom05g32280
rosa_chinensis RchiOBHm_Chr5g0001661
rosa_laevigata RLG00000030952
rosa_multiflora Rmu_ssc0000237.1_g000027
rosa_roxburghii Rroxscaffold_1G00074670
rosa_rugosa Rorug04G0391600
rosa_samantha Rh5AG013100 Rh5BG016400 Rh5CG014100 Rh5DG014200
rosa_wichuraiana Rw5G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccB1I GGYRCC 1 cut(s) 232
AciI CCGC 4 cut(s) 150, 153, 334, 509
AclWI GGATC 2 cut(s) 214, 317
AcsI RAATTY 1 cut(s) 187
AfaI GTAC 4 cut(s) 44, 99, 363, 588
AfiI CCNNNNNNNGG 2 cut(s) 39, 694
AflIII ACRYGT 1 cut(s) 100
AgeI ACCGGT 1 cut(s) 70
AgsI TTSAA 3 cut(s) 343, 575, 625
AjiI CACGTC 1 cut(s) 103
AluBI AGCT 2 cut(s) 138, 164
AluI AGCT 2 cut(s) 138, 164
Alw21I GWGCWC 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 109, 246
Alw44I GTGCAC 1 cut(s) 15
AlwI GGATC 2 cut(s) 214, 317
ApaLI GTGCAC 1 cut(s) 15
ApeKI GCWGC 3 cut(s) 155, 158, 161
ApoI RAATTY 1 cut(s) 187
AsiGI ACCGGT 1 cut(s) 70
Asp700I GAANNNNTTC 1 cut(s) 561
AspS9I GGNCC 3 cut(s) 30, 47, 68
AsuHPI GGTGA 2 cut(s) 55, 85
AsuII TTCGAA 1 cut(s) 185
AvaII GGWCC 3 cut(s) 30, 47, 68
BaeGI GKGCMC 2 cut(s) 19, 235
BanI GGYRCC 1 cut(s) 232
BbsI GAAGAC 1 cut(s) 465
Bbv12I GWGCWC 1 cut(s) 19
BbvI GCAGC 3 cut(s) 142, 145, 173
BccI CCATC 2 cut(s) 260, 547
BceAI ACGGC 2 cut(s) 271, 347
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciVI GTATCC 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 109, 246
BfmI CTRYAG 2 cut(s) 159, 483
BfuAI ACCTGC 1 cut(s) 559
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 4 cut(s) 153, 156, 159, 162
BlsI GCNGC 4 cut(s) 154, 157, 160, 163
BmcAI AGTACT 1 cut(s) 363
Bme18I GGWCC 3 cut(s) 30, 47, 68
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 30, 47, 68
BmiI GGNNCC 2 cut(s) 148, 234
BmsI GCATC 2 cut(s) 658, 664
BpiI GAAGAC 1 cut(s) 465
BplI GAGNNNNNCTC 2 cut(s) 271, 303
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 1 cut(s) 386
BsaJI CCNNGG 1 cut(s) 329
BsaWI WCCGGW 3 cut(s) 65, 70, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 694
Bse118I RCCGGY 2 cut(s) 49, 70
Bse3DI GCAATG 1 cut(s) 265
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 2 cut(s) 427, 598
BseLI CCNNNNNNNGG 2 cut(s) 39, 694
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 257
BseRI GAGGAG 3 cut(s) 130, 431, 503
BseSI GKGCMC 2 cut(s) 19, 235
BseXI GCAGC 3 cut(s) 142, 145, 173
BshNI GGYRCC 1 cut(s) 232
BshTI ACCGGT 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 19
BsiSI CCGG 4 cut(s) 50, 66, 71, 144
BslI CCNNNNNNNGG 2 cut(s) 39, 694
BsmAI GTCTC 2 cut(s) 109, 246
BsmBI CGTCTC 1 cut(s) 109
Bsp119I TTCGAA 1 cut(s) 185
Bsp1286I GDGCHC 2 cut(s) 19, 235
Bsp143I GATC 2 cut(s) 206, 322
BspACI CCGC 4 cut(s) 150, 153, 334, 509
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 148, 234
BspMAI CTGCAG 1 cut(s) 163
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 2 cut(s) 214, 317
BspT104I TTCGAA 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 2 cut(s) 49, 70
BssAI RCCGGY 2 cut(s) 49, 70
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 2 cut(s) 206, 322
Bst4CI ACNGT 9 cut(s) 21, 42, 58, 179, 197, 304, 313, 484, 637
BstAPI GCANNNNNTGC 2 cut(s) 217, 668
BstBI TTCGAA 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 117, 243
BstDSI CCRYGG 1 cut(s) 329
BstF5I GGATG 2 cut(s) 427, 598
BstKTI GATC 2 cut(s) 209, 325
BstMAI GTCTC 2 cut(s) 109, 246
BstMBI GATC 2 cut(s) 206, 322
BstMWI GCNNNNNNNGC 5 cut(s) 158, 161, 217, 314, 668
BstSFI CTRYAG 2 cut(s) 159, 483
BstSLI GKGCMC 2 cut(s) 19, 235
BstV1I GCAGC 3 cut(s) 142, 145, 173
BstV2I GAAGAC 1 cut(s) 465
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuI GTATCC 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 329
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 2 cut(s) 427, 598
BtsIMutI CAGTG 1 cut(s) 633
BveI ACCTGC 1 cut(s) 559
Cfr10I RCCGGY 2 cut(s) 49, 70
Cfr13I GGNCC 3 cut(s) 30, 47, 68
CpoI CGGWCCG 2 cut(s) 47, 68
Csp6I GTAC 4 cut(s) 43, 98, 362, 587
CspAI ACCGGT 1 cut(s) 70
CspI CGGWCCG 2 cut(s) 47, 68
CviJI RGCY 4 cut(s) 138, 164, 532, 703
CviKI_1 RGCY 4 cut(s) 138, 164, 532, 703
CviQI GTAC 4 cut(s) 43, 98, 362, 587
DdeI CTNAG 2 cut(s) 117, 243
DpnI GATC 2 cut(s) 208, 324
DpnII GATC 2 cut(s) 206, 322
EciI GGCGGA 2 cut(s) 139, 349
Eco47I GGWCC 3 cut(s) 30, 47, 68
EcoO109I RGGNCCY 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 109
FaiI YATR 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 153, 156, 159, 162
FokI GGATG 2 cut(s) 434, 605
Fsp4HI GCNGC 4 cut(s) 153, 156, 159, 162
GluI GCNGC 4 cut(s) 153, 156, 159, 162
HapII CCGG 4 cut(s) 50, 66, 71, 144
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 2 cut(s) 339, 491
HpaI GTTAAC 1 cut(s) 175
HpaII CCGG 4 cut(s) 50, 66, 71, 144
HphI GGTGA 2 cut(s) 55, 85
Hpy166II GTNNAC 3 cut(s) 17, 100, 175
Hpy188I TCNGA 5 cut(s) 193, 246, 291, 354, 552
Hpy8I GTNNAC 3 cut(s) 17, 100, 175
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 120, 373
HpyCH4III ACNGT 9 cut(s) 21, 42, 58, 179, 197, 304, 313, 484, 637
HpyCH4IV ACGT 2 cut(s) 102, 348
HpyCH4V TGCA 4 cut(s) 17, 161, 662, 677
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 161, 217, 314, 668
HpyF3I CTNAG 2 cut(s) 117, 243
HpySE526I ACGT 2 cut(s) 102, 348
KspAI GTTAAC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 206, 322
LmnI GCTCC 1 cut(s) 143
LpnPI CCDG 8 cut(s) 63, 79, 84, 157, 440, 546, 554, 575
Lsp1109I GCAGC 3 cut(s) 142, 145, 173
LweI GCATC 2 cut(s) 658, 664
MaeII ACGT 2 cut(s) 102, 348
MaeIII GTNAC 1 cut(s) 251
MalI GATC 2 cut(s) 208, 324
MboI GATC 2 cut(s) 206, 322
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 19, 235
MluCI AATT 4 cut(s) 187, 283, 384, 393
MlyI GAGTC 1 cut(s) 500
MroXI GAANNNNTTC 1 cut(s) 561
MseI TTAA 2 cut(s) 174, 656
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 50, 66, 71, 144
MwoI GCNNNNNNNGC 5 cut(s) 158, 161, 217, 314, 668
NdeII GATC 2 cut(s) 206, 322
NlaIV GGNNCC 2 cut(s) 148, 234
NspV TTCGAA 1 cut(s) 185
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 1 cut(s) 339
PinAI ACCGGT 1 cut(s) 70
PkrI GCNGC 4 cut(s) 154, 157, 160, 163
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PpuMI RGGWCCY 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 148, 234
PspPI GGNCC 3 cut(s) 30, 47, 68
PspPPI RGGWCCY 1 cut(s) 30
PstI CTGCAG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 4 cut(s) 44, 99, 363, 588
RsaNI GTAC 4 cut(s) 43, 98, 362, 587
Rsr2I CGGWCCG 2 cut(s) 47, 68
RsrII CGGWCCG 2 cut(s) 47, 68
SaqAI TTAA 2 cut(s) 174, 656
SatI GCNGC 4 cut(s) 153, 156, 159, 162
Sau3AI GATC 2 cut(s) 206, 322
Sau96I GGNCC 3 cut(s) 30, 47, 68
ScaI AGTACT 1 cut(s) 363
SchI GAGTC 1 cut(s) 500
SduI GDGCHC 2 cut(s) 19, 235
SetI ASST 9 cut(s) 28, 32, 105, 140, 166, 351, 450, 468, 573
SfaNI GCATC 2 cut(s) 658, 664
SfcI CTRYAG 2 cut(s) 159, 483
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 3 cut(s) 30, 47, 68
SmlI CTYRAG 1 cut(s) 365
SmoI CTYRAG 1 cut(s) 365
Sse9I AATT 4 cut(s) 187, 283, 384, 393
SsiI CCGC 4 cut(s) 150, 153, 334, 509
TaaI ACNGT 9 cut(s) 21, 42, 58, 179, 197, 304, 313, 484, 637
TaiI ACGT 2 cut(s) 105, 351
TaqI TCGA 3 cut(s) 35, 185, 494
TasI AATT 4 cut(s) 187, 283, 384, 393
TatI WGTACW 2 cut(s) 361, 586
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 2 cut(s) 174, 656
Tru9I TTAA 2 cut(s) 174, 656
TscAI CASTG 1 cut(s) 640
TseI GCWGC 3 cut(s) 155, 158, 161
TspDTI ATGAA 2 cut(s) 276, 612
TspGWI ACGGA 2 cut(s) 60, 157
TspRI CASTG 1 cut(s) 640
VneI GTGCAC 1 cut(s) 15
VpaK11BI GGWCC 3 cut(s) 30, 47, 68
XapI RAATTY 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 561
ZrmI AGTACT 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.