pycom05g32280

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0013927
Physical Location & Seq
Reverse (-)
31739709 .. 31742176
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32280.3

Sequence Viewer

Length: 627 bp
ATGGAGGGAGTTGGGGCCCGACTCGGGCGCACCTCGACCCGGCACGGACCGGCAACGGTGTTTACTGGGCCGGTGAGGAAGTGGAAGAAGAAGTGGGTCCACGTAGCTCCTCCCTCCAACCACCACACCTCCATCCATCACTCTCACCAGATTAACGGTTCCTCTAACGGTAATAACGGCTCGCATCTCCTGCTTTTCAAGTGGACCCCGATTGCCCAAAGCCAAAACACTGCCATTAGTAATGGCGCAGTCGCCAATGGAGGGGACAGGGACAAGGTTTCTGCCAAGGACGACGGCGCGTTGGCAGCGAGCGAGGAACCGCCCCGCCGAAAATTCAAATACATTCCGATTGCTTTACTAGAAGAAGAGCAAAATGAGGATGAAGAGCAGGTTGAAGATGAAGGTAACACTATCGATAATGATGCAGTTGACACAGAGGCAACCCCCAAGAATCAGGGTTTAGATGAAAAACCCGATATTAATGACGTTCCTATGGAAGAAAATCAGGAGAATAATCAGGTGGTATGCCAAGATCTGAATGAAACGTTGGAGTTAAGTTTGGGCTCGGATGGGCATGAAAATGACAACGATTCTGGTCTGAAAGCTGAACAAACCAGAAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.72

Weight (kDa)

4.87

Isoelectric Point (pI)

25.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22320 AT4G22320
fragaria_vesca FvH4_3g01090
malus_domestica MD05G1356800.v1.1 MD10G1329900.v1.1
prunus_persica Prupe.4G012100_v2.0.a1
pyrus_communis pycom05g32280
rosa_chinensis RchiOBHm_Chr5g0001661
rosa_laevigata RLG00000030952
rosa_multiflora Rmu_ssc0000237.1_g000027
rosa_roxburghii Rroxscaffold_1G00074670
rosa_rugosa Rorug04G0391600
rosa_samantha Rh5AG013100 Rh5BG016400 Rh5CG014100 Rh5DG014200
rosa_wichuraiana Rw5G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 379
AccII CGCG 1 cut(s) 299
AciI CCGC 2 cut(s) 320, 325
AclI AACGTT 1 cut(s) 545
AcsI RAATTY 1 cut(s) 332
AfiI CCNNNNNNNGG 3 cut(s) 24, 39, 261
AgsI TTSAA 3 cut(s) 199, 337, 395
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 2 cut(s) 107, 605
AluI AGCT 2 cut(s) 107, 605
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 2 cut(s) 15, 68
ApaI GGGCCC 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 1 cut(s) 332
AseI ATTAAT 1 cut(s) 480
AspLEI GCGC 3 cut(s) 30, 248, 299
AspS9I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 85, 137
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 3 cut(s) 47, 97, 204
BaeGI GKGCMC 1 cut(s) 19
BanII GRGCYC 2 cut(s) 19, 566
BbvI GCAGC 1 cut(s) 317
BccI CCATC 3 cut(s) 140, 144, 563
BceAI ACGGC 2 cut(s) 193, 310
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BcnI CCSGG 1 cut(s) 40
BfaI CTAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 532
BisI GCNGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 3 cut(s) 47, 97, 204
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
BmiI GGNNCC 6 cut(s) 16, 17, 98, 160, 206, 318
BmrFI CCNGG 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 193, 412
BmuI ACTGGG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 414
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 39, 261
Bse118I RCCGGY 2 cut(s) 49, 70
Bse1I ACTGG 1 cut(s) 70
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 3 cut(s) 132, 385, 574
BseLI CCNNNNNNNGG 3 cut(s) 24, 39, 261
BseNI ACTGG 1 cut(s) 70
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 1 cut(s) 317
Bsh1236I CGCG 1 cut(s) 299
BshFI GGCC 2 cut(s) 17, 70
BshVI ATCGAT 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 3 cut(s) 40, 50, 71
BslFI GGGAC 2 cut(s) 278, 284
BslI CCNNNNNNNGG 3 cut(s) 24, 39, 261
BsmFI GGGAC 2 cut(s) 278, 284
BsnI GGCC 2 cut(s) 17, 70
BsoBI CYCGRG 1 cut(s) 23
Bsp120I GGGCCC 1 cut(s) 15
Bsp1286I GDGCHC 2 cut(s) 19, 566
Bsp143I GATC 1 cut(s) 532
BspACI CCGC 2 cut(s) 320, 325
BspANI GGCC 2 cut(s) 17, 70
BspDI ATCGAT 1 cut(s) 414
BspFNI CGCG 1 cut(s) 299
BspLI GGNNCC 6 cut(s) 16, 17, 98, 160, 206, 318
BspMI ACCTGC 1 cut(s) 379
BspQI GCTCTTC 2 cut(s) 360, 378
BsrFI RCCGGY 2 cut(s) 49, 70
BsrI ACTGG 1 cut(s) 70
BssAI RCCGGY 2 cut(s) 49, 70
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 58, 158, 170
Bst6I CTCTTC 2 cut(s) 360, 378
BstAPI GCANNNNNTGC 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 182, 310
BstF5I GGATG 3 cut(s) 132, 385, 574
BstFNI CGCG 1 cut(s) 299
BstHHI GCGC 3 cut(s) 30, 248, 299
BstKTI GATC 1 cut(s) 535
BstMBI GATC 1 cut(s) 532
BstMWI GCNNNNNNNGC 2 cut(s) 190, 305
BstSCI CCNGG 1 cut(s) 38
BstSLI GKGCMC 1 cut(s) 19
BstUI CGCG 1 cut(s) 299
BstV1I GCAGC 1 cut(s) 317
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 2 cut(s) 17, 70
BsuTUI ATCGAT 1 cut(s) 414
BtsCI GGATG 3 cut(s) 132, 385, 574
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 2 cut(s) 182, 310
CfoI GCGC 3 cut(s) 30, 248, 299
Cfr10I RCCGGY 2 cut(s) 49, 70
Cfr13I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
ClaI ATCGAT 1 cut(s) 414
CpoI CGGWCCG 1 cut(s) 47
CspI CGGWCCG 1 cut(s) 47
CviAII CATG 1 cut(s) 575
CviJI RGCY 7 cut(s) 17, 70, 107, 180, 222, 564, 605
CviKI_1 RGCY 7 cut(s) 17, 70, 107, 180, 222, 564, 605
DpnI GATC 1 cut(s) 534
DpnII GATC 1 cut(s) 532
Eam1104I CTCTTC 2 cut(s) 360, 378
EarI CTCTTC 2 cut(s) 360, 378
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 2 cut(s) 19, 566
Eco47I GGWCC 3 cut(s) 47, 97, 204
Eco88I CYCGRG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 2 cut(s) 19, 566
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 1 cut(s) 578
FaiI YATR 3 cut(s) 494, 526, 576
FaqI GGGAC 2 cut(s) 278, 284
FatI CATG 1 cut(s) 574
FauI CCCGC 1 cut(s) 332
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 3 cut(s) 119, 392, 581
FriOI GRGCYC 2 cut(s) 19, 566
Fsp4HI GCNGC 1 cut(s) 306
FspBI CTAG 1 cut(s) 359
GlaI GCGC 3 cut(s) 29, 247, 298
GluI GCNGC 1 cut(s) 306
HaeIII GGCC 2 cut(s) 17, 70
HapII CCGG 3 cut(s) 40, 50, 71
HhaI GCGC 3 cut(s) 30, 248, 299
Hin1II CATG 1 cut(s) 578
Hin6I GCGC 3 cut(s) 28, 246, 297
HinP1I GCGC 3 cut(s) 28, 246, 297
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 3 cut(s) 21, 451, 590
HpaII CCGG 3 cut(s) 40, 50, 71
HphI GGTGA 2 cut(s) 85, 137
Hpy166II GTNNAC 4 cut(s) 63, 100, 204, 430
Hpy188I TCNGA 4 cut(s) 348, 537, 568, 600
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 4 cut(s) 63, 100, 204, 430
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 1 cut(s) 395
HpyCH4III ACNGT 3 cut(s) 58, 158, 170
HpyCH4IV ACGT 3 cut(s) 102, 486, 545
HpyCH4V TGCA 1 cut(s) 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 305
HpySE526I ACGT 3 cut(s) 102, 486, 545
Hsp92II CATG 1 cut(s) 578
HspAI GCGC 3 cut(s) 28, 246, 297
Kzo9I GATC 1 cut(s) 532
LguI GCTCTTC 2 cut(s) 360, 378
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 1 cut(s) 317
LweI GCATC 2 cut(s) 193, 412
MaeI CTAG 1 cut(s) 359
MaeII ACGT 3 cut(s) 102, 486, 545
MaeIII GTNAC 1 cut(s) 404
MalI GATC 1 cut(s) 534
MboI GATC 1 cut(s) 532
MboII GAAGA 7 cut(s) 97, 100, 374, 377, 395, 407, 509
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 19, 566
MluCI AATT 1 cut(s) 332
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 2 cut(s) 141, 528
MseI TTAA 3 cut(s) 153, 480, 554
MslI CAYNNNNRTG 1 cut(s) 579
MspI CCGG 3 cut(s) 40, 50, 71
MspR9I CCNGG 1 cut(s) 40
MvnI CGCG 1 cut(s) 299
MwoI GCNNNNNNNGC 2 cut(s) 190, 305
NciI CCSGG 1 cut(s) 40
NdeII GATC 1 cut(s) 532
NlaIII CATG 1 cut(s) 578
NlaIV GGNNCC 6 cut(s) 16, 17, 98, 160, 206, 318
PciSI GCTCTTC 2 cut(s) 360, 378
PfeI GAWTC 2 cut(s) 451, 590
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 480
Psp1406I AACGTT 1 cut(s) 545
PspN4I GGNNCC 6 cut(s) 16, 17, 98, 160, 206, 318
PspOMI GGGCCC 1 cut(s) 15
PspPI GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
PsuI RGATCY 1 cut(s) 532
RseI CAYNNNNRTG 1 cut(s) 579
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 360, 378
SaqAI TTAA 3 cut(s) 153, 480, 554
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 1 cut(s) 532
Sau96I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 2 cut(s) 19, 566
SfaNI GCATC 2 cut(s) 193, 412
SinI GGWCC 3 cut(s) 47, 97, 204
SmiMI CAYNNNNRTG 1 cut(s) 579
Sse9I AATT 1 cut(s) 332
SsiI CCGC 2 cut(s) 320, 325
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 3 cut(s) 58, 158, 170
TaiI ACGT 3 cut(s) 105, 489, 548
TaqI TCGA 2 cut(s) 35, 414
TasI AATT 1 cut(s) 332
TfiI GAWTC 2 cut(s) 451, 590
Tru1I TTAA 3 cut(s) 153, 480, 554
Tru9I TTAA 3 cut(s) 153, 480, 554
TscAI CASTG 1 cut(s) 235
TseI GCWGC 1 cut(s) 305
TspDTI ATGAA 5 cut(s) 396, 414, 480, 555, 591
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 3 cut(s) 47, 97, 204
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 332
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.