MD10G1329900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013927
Physical Location & Seq
Reverse (-)
40836960 .. 40839979
3020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1329900.v1.1.491

Sequence Viewer

Length: 597 bp
ATGGAGGGAGTTGGGGCCCGACTCGGGCGGACTTCGACCCGGCACGGACCGGCCACGGTGTTCACTGGGCCGGTAAGGAAGTGGAAGAAGAAGTGGGTCCACGTAGCTCCTCCCTCCAACCACAACACCTCCACCCATCACTCTCACCAGATTAACGGTTCCTCTAACGGTATTAACGGCTCCCATCTCTTGCTTTACAAGTGGACCCCAATTGCCCAAAGCCAAAACGCTACCATTAGTAATGGTGCAGTCGCCAATGGAGGGGACAGGGACAAGGTTTCTGCCAAGGACGACGGCCTTTTGGCTGCGAGCGATGAGCCGCCCCGCCGAAAATTCAAATACATTCCGATTGCTTTACTAGAAGAACAGCAAAATGAGGCTGCAGAGCAGGTTGAAGATGACGGAAACTCTATCGATGATGAGCCAGTTGAAACACAGGCAACCCCAAAGAATGATGGTTTAGATGAAAAACCTGACATTAATGACGTTCCTATGGAAGAAAATCAGGAGAGTAATCAGGTAGTATGCCAAGATCTGAATTTAAGTTTGGGCTTGAATGGGCATGAAAACGATAACGATTCTGGTCTGAAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

21.47

Weight (kDa)

5.1

Isoelectric Point (pI)

24.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22320 AT4G22320
fragaria_vesca FvH4_3g01090
malus_domestica MD05G1356800.v1.1 MD10G1329900.v1.1
prunus_persica Prupe.4G012100_v2.0.a1
pyrus_communis pycom05g32280
rosa_chinensis RchiOBHm_Chr5g0001661
rosa_laevigata RLG00000030952
rosa_multiflora Rmu_ssc0000237.1_g000027
rosa_roxburghii Rroxscaffold_1G00074670
rosa_rugosa Rorug04G0391600
rosa_samantha Rh5AG013100 Rh5BG016400 Rh5CG014100 Rh5DG014200
rosa_wichuraiana Rw5G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 379
AciI CCGC 3 cut(s) 28, 320, 325
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 332, 538
AfiI CCNNNNNNNGG 2 cut(s) 24, 261
AgsI TTSAA 4 cut(s) 337, 395, 431, 556
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 2 cut(s) 107, 593
AluI AGCT 2 cut(s) 107, 593
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 4 cut(s) 15, 51, 68, 295
ApaI GGGCCC 1 cut(s) 19
ApeKI GCWGC 2 cut(s) 305, 380
ApoI RAATTY 2 cut(s) 332, 538
AseI ATTAAT 1 cut(s) 480
AspS9I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 1 cut(s) 137
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 3 cut(s) 47, 97, 204
BaeGI GKGCMC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 19
BbvI GCAGC 2 cut(s) 292, 367
BccI CCATC 3 cut(s) 144, 192, 449
BceAI ACGGC 2 cut(s) 193, 310
BcnI CCSGG 1 cut(s) 40
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 532
BisI GCNGC 3 cut(s) 306, 320, 381
BlsI GCNGC 3 cut(s) 307, 321, 382
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 3 cut(s) 47, 97, 204
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
BmiI GGNNCC 6 cut(s) 16, 17, 98, 160, 181, 206
BmrFI CCNGG 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 414
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 2 cut(s) 54, 285
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 261
Bse118I RCCGGY 2 cut(s) 49, 70
Bse1I ACTGG 2 cut(s) 70, 425
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 2 cut(s) 54, 285
BseLI CCNNNNNNNGG 2 cut(s) 24, 261
BseNI ACTGG 2 cut(s) 70, 425
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 2 cut(s) 292, 367
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 4 cut(s) 17, 53, 70, 297
BshVI ATCGAT 1 cut(s) 414
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 3 cut(s) 40, 50, 71
BslFI GGGAC 2 cut(s) 278, 284
BslI CCNNNNNNNGG 2 cut(s) 24, 261
BsmFI GGGAC 2 cut(s) 278, 284
BsnI GGCC 4 cut(s) 17, 53, 70, 297
BsoBI CYCGRG 1 cut(s) 23
Bsp120I GGGCCC 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 1 cut(s) 532
BspACI CCGC 3 cut(s) 28, 320, 325
BspANI GGCC 4 cut(s) 17, 53, 70, 297
BspDI ATCGAT 1 cut(s) 414
BspLI GGNNCC 6 cut(s) 16, 17, 98, 160, 181, 206
BspMAI CTGCAG 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 379
BsrFI RCCGGY 2 cut(s) 49, 70
BsrI ACTGG 2 cut(s) 70, 425
BssAI RCCGGY 2 cut(s) 49, 70
BssECI CCNNGG 2 cut(s) 54, 285
BssMI GATC 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 58, 158, 170
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 310
BstDSI CCRYGG 1 cut(s) 54
BstKTI GATC 1 cut(s) 535
BstMBI GATC 1 cut(s) 532
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 19
BstV1I GCAGC 2 cut(s) 292, 367
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 4 cut(s) 17, 53, 70, 297
BsuTUI ATCGAT 1 cut(s) 414
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 63
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 1 cut(s) 310
Cfr10I RCCGGY 2 cut(s) 49, 70
Cfr13I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
ClaI ATCGAT 1 cut(s) 414
CpoI CGGWCCG 1 cut(s) 47
CspI CGGWCCG 1 cut(s) 47
CviAII CATG 1 cut(s) 563
DpnI GATC 1 cut(s) 534
DpnII GATC 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 3 cut(s) 47, 97, 204
Eco88I CYCGRG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 285
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 285
FaeI CATG 1 cut(s) 566
FaiI YATR 3 cut(s) 494, 526, 564
FaqI GGGAC 2 cut(s) 278, 284
FatI CATG 1 cut(s) 562
FauI CCCGC 1 cut(s) 332
Fnu4HI GCNGC 3 cut(s) 306, 320, 381
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 3 cut(s) 306, 320, 381
FspBI CTAG 1 cut(s) 359
GluI GCNGC 3 cut(s) 306, 320, 381
HaeIII GGCC 4 cut(s) 17, 53, 70, 297
HapII CCGG 3 cut(s) 40, 50, 71
Hin1II CATG 1 cut(s) 566
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 2 cut(s) 21, 578
HpaII CCGG 3 cut(s) 40, 50, 71
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 3 cut(s) 63, 100, 204
Hpy188I TCNGA 3 cut(s) 348, 537, 588
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 3 cut(s) 63, 100, 204
Hpy99I CGWCG 1 cut(s) 296
HpyCH4III ACNGT 3 cut(s) 58, 158, 170
HpyCH4IV ACGT 2 cut(s) 102, 486
HpyCH4V TGCA 2 cut(s) 248, 383
HpySE526I ACGT 2 cut(s) 102, 486
Hsp92II CATG 1 cut(s) 566
Kzo9I GATC 1 cut(s) 532
LmnI GCTCC 2 cut(s) 112, 185
Lsp1109I GCAGC 2 cut(s) 292, 367
MaeI CTAG 1 cut(s) 359
MaeII ACGT 2 cut(s) 102, 486
MalI GATC 1 cut(s) 534
MboI GATC 1 cut(s) 532
MboII GAAGA 5 cut(s) 97, 100, 374, 407, 509
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 3 cut(s) 210, 332, 538
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 6 cut(s) 120, 124, 139, 172, 254, 370
MseI TTAA 5 cut(s) 153, 174, 480, 542, 595
MspI CCGG 3 cut(s) 40, 50, 71
MspR9I CCNGG 1 cut(s) 40
MunI CAATTG 1 cut(s) 210
NciI CCSGG 1 cut(s) 40
NdeII GATC 1 cut(s) 532
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 6 cut(s) 16, 17, 98, 160, 181, 206
PfeI GAWTC 1 cut(s) 578
PkrI GCNGC 3 cut(s) 307, 321, 382
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 480
PspN4I GGNNCC 6 cut(s) 16, 17, 98, 160, 181, 206
PspOMI GGGCCC 1 cut(s) 15
PspPI GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 1 cut(s) 532
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SaqAI TTAA 5 cut(s) 153, 174, 480, 542, 595
SatI GCNGC 3 cut(s) 306, 320, 381
Sau3AI GATC 1 cut(s) 532
Sau96I GGNCC 6 cut(s) 15, 16, 47, 68, 97, 204
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 1 cut(s) 19
SetI ASST 9 cut(s) 105, 109, 131, 279, 393, 475, 489, 522, 595
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 3 cut(s) 47, 97, 204
Sse9I AATT 3 cut(s) 210, 332, 538
SsiI CCGC 3 cut(s) 28, 320, 325
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 1 cut(s) 285
TaaI ACNGT 3 cut(s) 58, 158, 170
TaiI ACGT 2 cut(s) 105, 489
TaqI TCGA 2 cut(s) 35, 414
TasI AATT 3 cut(s) 210, 332, 538
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 1 cut(s) 578
Tru1I TTAA 5 cut(s) 153, 174, 480, 542, 595
Tru9I TTAA 5 cut(s) 153, 174, 480, 542, 595
TscAI CASTG 1 cut(s) 70
TseI GCWGC 2 cut(s) 305, 380
TspDTI ATGAA 2 cut(s) 480, 579
TspGWI ACGGA 2 cut(s) 60, 417
TspRI CASTG 1 cut(s) 70
VpaK11BI GGWCC 3 cut(s) 47, 97, 204
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 2 cut(s) 332, 538
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.