MD05G1356800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013927
Physical Location & Seq
Reverse (-)
47233209 .. 47236333
3125 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1356800.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGAGGGAGTTGGGGCCCGACTCGGGCGCACCTCGACCCGGCACGGACCGGCAACGGTGTTTACTGGGCCGGTGAGGAAGTGGAAGAAGAAGTGGGTACACGTAGCTCCTCCCTCCAACCACAACACCTCCATCCATCACTCTCACCAGATTAACGGTTCCTCTAATGGTAATAACGGCTCGCATCTCCTGCTTTTCAAGTGGACCCCGATTGCCATTAGTAATGGCGCAGTCGCCAATGGAGGGGACAGGGACAAGGTTTCTGCCAAGGACGACGGCGCTTTGGCAGCGAGCGAGGAACCGCCCCGCCGAAAATTCAAATACATTCCGATTGCTTTACTAGAAGAAGAGCAAAATGAGGCTACAGAAGATGAAGGTAACTCAATTGATAATGATCCAGTTGATACAGAGGCAAACCCCAAGAATCAGGGTTTAGATGAAAAACCCGATATTAATGACGTTCCTATGGAAGAAAATCAGGAGAATAATCAGGTAGTATGCCAAGATCTGAATGAAACGTTGGAGTTAAGTTTGGGCTCTGATGGGCATGAAAACGACAACGATTCTGGTCTGAAAGCTGAACAAACCAGAAATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

21.66

Weight (kDa)

4.98

Isoelectric Point (pI)

23.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22320 AT4G22320
fragaria_vesca FvH4_3g01090
malus_domestica MD05G1356800.v1.1 MD10G1329900.v1.1
prunus_persica Prupe.4G012100_v2.0.a1
pyrus_communis pycom05g32280
rosa_chinensis RchiOBHm_Chr5g0001661
rosa_laevigata RLG00000030952
rosa_multiflora Rmu_ssc0000237.1_g000027
rosa_roxburghii Rroxscaffold_1G00074670
rosa_rugosa Rorug04G0391600
rosa_samantha Rh5AG013100 Rh5BG016400 Rh5CG014100 Rh5DG014200
rosa_wichuraiana Rw5G001260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 302, 307
AclI AACGTT 1 cut(s) 518
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 1 cut(s) 314
AfaI GTAC 1 cut(s) 99
AfiI CCNNNNNNNGG 3 cut(s) 24, 39, 243
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 2 cut(s) 199, 319
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AluBI AGCT 2 cut(s) 107, 578
AluI AGCT 2 cut(s) 107, 578
AlwI GGATC 1 cut(s) 389
Ama87I CYCGRG 1 cut(s) 23
AoxI GGCC 2 cut(s) 15, 68
ApaI GGGCCC 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 314
AseI ATTAAT 1 cut(s) 453
AspLEI GCGC 3 cut(s) 30, 230, 281
AspS9I GGNCC 5 cut(s) 15, 16, 47, 68, 204
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 85, 137
AvaI CYCGRG 1 cut(s) 23
AvaII GGWCC 2 cut(s) 47, 204
BaeGI GKGCMC 1 cut(s) 19
BanII GRGCYC 2 cut(s) 19, 539
BbvI GCAGC 1 cut(s) 299
BccI CCATC 3 cut(s) 140, 144, 536
BceAI ACGGC 2 cut(s) 193, 292
BcnI CCSGG 1 cut(s) 40
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 282
BglII AGATCT 1 cut(s) 505
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 2 cut(s) 47, 204
BmeT110I CYCGRG 1 cut(s) 23
BmgT120I GGNCC 5 cut(s) 15, 16, 47, 68, 204
BmiI GGNNCC 5 cut(s) 16, 17, 160, 206, 300
BmrFI CCNGG 1 cut(s) 40
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 193
BmuI ACTGGG 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 393
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 39, 243
Bse118I RCCGGY 2 cut(s) 49, 70
Bse1I ACTGG 2 cut(s) 70, 398
Bse8I GATNNNNATC 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 1 cut(s) 132
BseJI GATNNNNATC 1 cut(s) 393
BseLI CCNNNNNNNGG 3 cut(s) 24, 39, 243
BseNI ACTGG 2 cut(s) 70, 398
BseRI GAGGAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 1 cut(s) 299
BshFI GGCC 2 cut(s) 17, 70
BsiHKCI CYCGRG 1 cut(s) 23
BsiSI CCGG 3 cut(s) 40, 50, 71
BslFI GGGAC 2 cut(s) 260, 266
BslI CCNNNNNNNGG 3 cut(s) 24, 39, 243
BsmFI GGGAC 2 cut(s) 260, 266
BsnI GGCC 2 cut(s) 17, 70
BsoBI CYCGRG 1 cut(s) 23
Bsp120I GGGCCC 1 cut(s) 15
Bsp1286I GDGCHC 2 cut(s) 19, 539
Bsp143I GATC 2 cut(s) 394, 505
BspACI CCGC 2 cut(s) 302, 307
BspANI GGCC 2 cut(s) 17, 70
BspLI GGNNCC 5 cut(s) 16, 17, 160, 206, 300
BspPI GGATC 1 cut(s) 389
BspQI GCTCTTC 1 cut(s) 342
BsrFI RCCGGY 2 cut(s) 49, 70
BsrI ACTGG 2 cut(s) 70, 398
BssAI RCCGGY 2 cut(s) 49, 70
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 2 cut(s) 394, 505
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 58, 158
Bst6I CTCTTC 1 cut(s) 342
BstAPI GCANNNNNTGC 1 cut(s) 190
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 2 cut(s) 182, 292
BstF5I GGATG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 3 cut(s) 30, 230, 281
BstKTI GATC 2 cut(s) 397, 508
BstMBI GATC 2 cut(s) 394, 505
BstMWI GCNNNNNNNGC 2 cut(s) 190, 287
BstSCI CCNGG 1 cut(s) 38
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 505
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 2 cut(s) 17, 70
BtsCI GGATG 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 182, 292
CfoI GCGC 3 cut(s) 30, 230, 281
Cfr10I RCCGGY 2 cut(s) 49, 70
Cfr13I GGNCC 5 cut(s) 15, 16, 47, 68, 204
CpoI CGGWCCG 1 cut(s) 47
Csp6I GTAC 1 cut(s) 98
CspI CGGWCCG 1 cut(s) 47
CviAII CATG 1 cut(s) 548
CviJI RGCY 7 cut(s) 17, 70, 107, 180, 362, 537, 578
CviKI_1 RGCY 7 cut(s) 17, 70, 107, 180, 362, 537, 578
CviQI GTAC 1 cut(s) 98
DpnI GATC 2 cut(s) 396, 507
DpnII GATC 2 cut(s) 394, 505
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 2 cut(s) 19, 539
Eco47I GGWCC 2 cut(s) 47, 204
Eco88I CYCGRG 1 cut(s) 23
EcoO109I RGGNCCY 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 19, 539
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 1 cut(s) 551
FaiI YATR 3 cut(s) 467, 499, 549
FaqI GGGAC 2 cut(s) 260, 266
FatI CATG 1 cut(s) 547
FauI CCCGC 1 cut(s) 314
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 2 cut(s) 19, 539
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 341
GlaI GCGC 3 cut(s) 29, 229, 280
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 2 cut(s) 17, 70
HapII CCGG 3 cut(s) 40, 50, 71
HhaI GCGC 3 cut(s) 30, 230, 281
Hin1II CATG 1 cut(s) 551
Hin6I GCGC 3 cut(s) 28, 228, 279
HinP1I GCGC 3 cut(s) 28, 228, 279
HinfI GANTC 3 cut(s) 21, 424, 563
HpaII CCGG 3 cut(s) 40, 50, 71
HphI GGTGA 2 cut(s) 85, 137
Hpy166II GTNNAC 3 cut(s) 63, 100, 204
Hpy188I TCNGA 4 cut(s) 330, 510, 541, 573
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 3 cut(s) 63, 100, 204
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 1 cut(s) 368
HpyCH4III ACNGT 2 cut(s) 58, 158
HpyCH4IV ACGT 3 cut(s) 102, 459, 518
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 287
HpySE526I ACGT 3 cut(s) 102, 459, 518
Hsp92II CATG 1 cut(s) 551
HspAI GCGC 3 cut(s) 28, 228, 279
Kzo9I GATC 2 cut(s) 394, 505
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 1 cut(s) 112
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 341
MaeII ACGT 3 cut(s) 102, 459, 518
MaeIII GTNAC 1 cut(s) 377
MalI GATC 2 cut(s) 396, 507
MboI GATC 2 cut(s) 394, 505
MboII GAAGA 6 cut(s) 97, 100, 356, 359, 380, 482
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 19, 539
MluCI AATT 2 cut(s) 314, 384
MlyI GAGTC 1 cut(s) 15
MmeI TCCRAC 2 cut(s) 141, 501
MseI TTAA 3 cut(s) 153, 453, 527
MspI CCGG 3 cut(s) 40, 50, 71
MspR9I CCNGG 1 cut(s) 40
MunI CAATTG 1 cut(s) 384
MwoI GCNNNNNNNGC 2 cut(s) 190, 287
NciI CCSGG 1 cut(s) 40
NdeII GATC 2 cut(s) 394, 505
NlaIII CATG 1 cut(s) 551
NlaIV GGNNCC 5 cut(s) 16, 17, 160, 206, 300
PciSI GCTCTTC 1 cut(s) 342
PfeI GAWTC 2 cut(s) 424, 563
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
Ppu21I YACGTR 1 cut(s) 103
PshBI ATTAAT 1 cut(s) 453
Psp1406I AACGTT 1 cut(s) 518
PspN4I GGNNCC 5 cut(s) 16, 17, 160, 206, 300
PspOMI GGGCCC 1 cut(s) 15
PspPI GGNCC 5 cut(s) 15, 16, 47, 68, 204
PsuI RGATCY 1 cut(s) 505
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 3 cut(s) 153, 453, 527
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 2 cut(s) 394, 505
Sau96I GGNCC 5 cut(s) 15, 16, 47, 68, 204
SchI GAGTC 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 2 cut(s) 19, 539
SfaNI GCATC 1 cut(s) 193
SfcI CTRYAG 1 cut(s) 363
SinI GGWCC 2 cut(s) 47, 204
Sse9I AATT 2 cut(s) 314, 384
SsiI CCGC 2 cut(s) 302, 307
SspMI CTAG 1 cut(s) 341
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 58, 158
TaiI ACGT 3 cut(s) 105, 462, 521
TaqI TCGA 1 cut(s) 35
TasI AATT 2 cut(s) 314, 384
TfiI GAWTC 2 cut(s) 424, 563
Tru1I TTAA 3 cut(s) 153, 453, 527
Tru9I TTAA 3 cut(s) 153, 453, 527
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 4 cut(s) 387, 453, 528, 564
TspGWI ACGGA 1 cut(s) 60
VpaK11BI GGWCC 2 cut(s) 47, 204
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.