AT4G24440

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008441
Physical Location & Seq
Forward (+)
12633180 .. 12634784
1605 bp
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UTR
Exon/CDS
Intron
AT4G24440.2

Sequence Viewer

Length: 321 bp
ATGGCGACGTTTGAGCTGTACAGGAGATCGACGATCGGGATGTGTCTGACGGAGACTTTGGACGAGATGGTTCAGAGCGGTACGCTGAGCCCTGAGCTAGCTATCCAAGTCCTTGTTCAATTTGACAAGTCCATGACTGAAGCTCTGGAGAGCCAAGTGAAGACCAAGGTGTCCATCAAGGGACACCTGCACACTTACAGGTTCTGTGACAATGTCTGGACCTTCATATTACAGGACGCAATGTTCAAGAGTGACGATCGTCAAGAGAATGTGAGCCGGGTGAAGATAGTGGCATGTGATTCCAAGCTGCTCACACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0003674 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005672 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016591 GO:0017025 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0060255 GO:0061695 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05203 map03022 map05203
Pfam Domains
Protein Families
TFIIA_gamma_C TFIIA_gamma_N

Protein Analysis

106

Amino Acids

12.14

Weight (kDa)

5.61

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIA_gamma_N PF02268 4 - 49 1.8e-22 Transcription initiation factor IIA, gamma subunit, helical domain
TFIIA_gamma_C PF02751 60 - 101 2.3e-22 Transcription initiation factor IIA, gamma subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 195
AccBSI CCGCTC 1 cut(s) 78
AciI CCGC 1 cut(s) 78
AcuI CTGAAG 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 318
AfaI GTAC 2 cut(s) 20, 82
AgsI TTSAA 2 cut(s) 119, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 99, 131
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 5 cut(s) 16, 97, 101, 143, 307
AluI AGCT 5 cut(s) 16, 97, 101, 143, 307
Alw26I GTCTC 1 cut(s) 47
AlwNI CAGNNNCTG 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 292
AsuNHI GCTAGC 1 cut(s) 97
AvaII GGWCC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 92
BbsI GAAGAC 1 cut(s) 167
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 61, 182
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 98
BfuAI ACCTGC 1 cut(s) 195
BisI GCNGC 1 cut(s) 308
BlpI GCTNAGC 1 cut(s) 86
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 278
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 219
BmrFI CCNGG 1 cut(s) 278
BmtI GCTAGC 1 cut(s) 101
BoxI GACNNNNGTC 1 cut(s) 258
BpiI GAAGAC 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 2 cut(s) 77, 84
BseXI GCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 36, 259
BsiEI CGRYCG 2 cut(s) 36, 259
BsiSI CCGG 1 cut(s) 277
BslFI GGGAC 1 cut(s) 195
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 92
Bsp1407I TGTACA 1 cut(s) 18
Bsp143I GATC 3 cut(s) 26, 33, 256
Bsp1720I GCTNAGC 1 cut(s) 86
BspACI CCGC 1 cut(s) 78
BspCNI CTCAG 2 cut(s) 78, 85
BspMI ACCTGC 1 cut(s) 195
BspOI GCTAGC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 78
BsrDI GCAATG 1 cut(s) 246
BsrGI TGTACA 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 26, 33, 256
BssT1I CCWWGG 1 cut(s) 165
Bst4CI ACNGT 1 cut(s) 318
BstAUI TGTACA 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 2 cut(s) 86, 93
BstF5I GGATG 1 cut(s) 45
BstKTI GATC 3 cut(s) 29, 36, 259
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 26, 33, 256
BstMCI CGRYCG 2 cut(s) 36, 259
BstNSI RCATGY 1 cut(s) 297
BstPAI GACNNNNGTC 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 167
BtsCI GGATG 1 cut(s) 45
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 219
CseI GACGC 1 cut(s) 245
Csp6I GTAC 2 cut(s) 19, 81
CviAII CATG 2 cut(s) 133, 294
CviJI RGCY 8 cut(s) 16, 90, 97, 101, 143, 153, 276, 307
CviKI_1 RGCY 8 cut(s) 16, 90, 97, 101, 143, 153, 276, 307
CviQI GTAC 2 cut(s) 19, 81
DdeI CTNAG 2 cut(s) 86, 93
DpnI GATC 3 cut(s) 28, 35, 258
DpnII GATC 3 cut(s) 26, 33, 256
DraIII CACNNNGTG 1 cut(s) 318
DrdI GACNNNNNNGTC 1 cut(s) 169
DseDI GACNNNNNNGTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 92
Eco47I GGWCC 1 cut(s) 219
Eco57I CTGAAG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 92
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 136, 297
FaiI YATR 3 cut(s) 134, 227, 295
FaqI GGGAC 1 cut(s) 195
FatI CATG 2 cut(s) 132, 293
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 92
Fsp4HI GCNGC 1 cut(s) 308
FspBI CTAG 1 cut(s) 98
GluI GCNGC 1 cut(s) 308
GsuI CTGGAG 1 cut(s) 167
HapII CCGG 1 cut(s) 277
HgaI GACGC 1 cut(s) 245
Hin1II CATG 2 cut(s) 136, 297
HinfI GANTC 1 cut(s) 299
HpaII CCGG 1 cut(s) 277
HphI GGTGA 1 cut(s) 292
Hpy188I TCNGA 2 cut(s) 48, 75
Hpy188III TCNNGA 5 cut(s) 37, 146, 217, 247, 263
Hpy99I CGWCG 2 cut(s) 10, 34
HpyAV CCTTC 1 cut(s) 232
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 86, 93
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 2 cut(s) 136, 297
Kzo9I GATC 3 cut(s) 26, 33, 256
LpnPI CCDG 8 cut(s) 7, 105, 131, 184, 200, 202, 218, 290
Lsp1109I GCAGC 1 cut(s) 294
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 206, 251
MalI GATC 3 cut(s) 28, 35, 258
MbiI CCGCTC 1 cut(s) 78
MboI GATC 3 cut(s) 26, 33, 256
MboII GAAGA 2 cut(s) 172, 295
MhlI GDGCHC 1 cut(s) 92
MluCI AATT 1 cut(s) 119
MspI CCGG 1 cut(s) 277
MspR9I CCNGG 1 cut(s) 278
NciI CCSGG 1 cut(s) 278
NdeII GATC 3 cut(s) 26, 33, 256
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 2 cut(s) 136, 297
NmuCI GTSAC 2 cut(s) 206, 251
NspI RCATGY 1 cut(s) 297
PaqCI CACCTGC 1 cut(s) 195
PfeI GAWTC 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 212
PkrI GCNGC 1 cut(s) 309
Ple19I CGATCG 2 cut(s) 36, 259
PshAI GACNNNNGTC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 212
PvuI CGATCG 2 cut(s) 36, 259
RsaI GTAC 2 cut(s) 20, 82
RsaNI GTAC 2 cut(s) 19, 81
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 3 cut(s) 26, 33, 256
Sau96I GGNCC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 1 cut(s) 92
SinI GGWCC 1 cut(s) 219
Sse9I AATT 1 cut(s) 119
SsiI CCGC 1 cut(s) 78
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 1 cut(s) 11
TaqI TCGA 1 cut(s) 29
TasI AATT 1 cut(s) 119
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 1 cut(s) 299
TseFI GTSAC 2 cut(s) 206, 251
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 2 cut(s) 206, 251
TspDTI ATGAA 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 65
Tth111I GACNNNGTC 1 cut(s) 212
VpaK11BI GGWCC 1 cut(s) 219
XceI RCATGY 1 cut(s) 297
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.