FvH4_5g34180

TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008441
Physical Location & Seq
Forward (+)
24856823 .. 24861400
4578 bp
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UTR
Exon/CDS
Intron
FvH4_5g34180.t1

Sequence Viewer

Length: 321 bp
ATGGCGACGTTTGAGCTATACAGGAGGTCGACGATCGGAATGTGTTTGACTGAGACTTTGGACGAGATGGTTCAGAACGGGACTCTCAGTCCTGAGCTCGCAATTCAGGTTCTTGTTCAGTTCGATAAGTCTATGACTGAAGCTCTCGAAACCCAGGTGAAGAGCAAGGTCTCCATCAAGGGACATCTGCACACCTACAGATTCTGTGACAATGTTTGGACCTTCATCTTACAGGATGCTTTGTTTAAGAATGAGGATAGTCAGGAAAATGTTGGGCGTGTTAAAATAGTGGCATGTGACTCAAAGCTGCTCTCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0003674 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005672 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016591 GO:0017025 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022607 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043933 GO:0044085 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051090 GO:0051091 GO:0051123 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0060255 GO:0061695 GO:0065003 GO:0065004 GO:0065007 GO:0065009 GO:0070013 GO:0070897 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05203 map03022 map05203
Pfam Domains
Protein Families
TFIIA_gamma_C TFIIA_gamma_N

Protein Analysis

107

Amino Acids

12.08

Weight (kDa)

5.29

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIA_gamma_N PF02268 4 - 49 1.8e-22 Transcription initiation factor IIA, gamma subunit, helical domain
TFIIA_gamma_C PF02751 60 - 101 6.6e-22 Transcription initiation factor IIA, gamma subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 159
AhdI GACNNNNNGTC 1 cut(s) 87
AjnI CCWGG 1 cut(s) 153
AluBI AGCT 4 cut(s) 16, 97, 143, 307
AluI AGCT 4 cut(s) 16, 97, 143, 307
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 2 cut(s) 47, 175
AlwNI CAGNNNCTG 1 cut(s) 204
ApeKI GCWGC 1 cut(s) 307
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspS9I GGNCC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 169
AvaII GGWCC 1 cut(s) 219
BanII GRGCYC 1 cut(s) 99
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 61, 182
BciT130I CCWGG 1 cut(s) 155
BcoDI GTCTC 2 cut(s) 47, 175
BfmI CTRYAG 1 cut(s) 196
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 1 cut(s) 219
BmeRI GACNNNNNGTC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 219
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 1 cut(s) 226
Bpu10I CCTNAGC 1 cut(s) 93
BsaI GGTCTC 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 153
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 1 cut(s) 241
BseMII CTCAG 3 cut(s) 42, 84, 100
BseXI GCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 173
Bsh1285I CGRYCG 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 99
BslFI GGGAC 2 cut(s) 94, 195
BsmAI GTCTC 2 cut(s) 47, 175
BsmFI GGGAC 2 cut(s) 94, 195
Bso31I GGTCTC 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 1 cut(s) 33
BspCNI CTCAG 3 cut(s) 43, 85, 99
BspQI GCTCTTC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 3 cut(s) 51, 86, 93
BstF5I GGATG 1 cut(s) 241
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 2 cut(s) 47, 175
BstMBI GATC 1 cut(s) 33
BstMCI CGRYCG 1 cut(s) 36
BstNI CCWGG 1 cut(s) 155
BstNSI RCATGY 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 153
BstSFI CTRYAG 1 cut(s) 196
BstV1I GCAGC 1 cut(s) 294
BtsCI GGATG 1 cut(s) 241
Cac8I GCNNGC 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 219
CviAII CATG 1 cut(s) 294
CviJI RGCY 4 cut(s) 16, 97, 143, 307
CviKI_1 RGCY 4 cut(s) 16, 97, 143, 307
DdeI CTNAG 3 cut(s) 51, 86, 93
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DriI GACNNNNNGTC 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 153
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 1 cut(s) 297
FaiI YATR 3 cut(s) 19, 134, 295
FaqI GGGAC 2 cut(s) 94, 195
FatI CATG 1 cut(s) 293
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 1 cut(s) 248
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 308
Hin1II CATG 1 cut(s) 297
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 3 cut(s) 82, 201, 299
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 38, 75
Hpy188III TCNNGA 3 cut(s) 92, 146, 263
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 2 cut(s) 10, 34
HpyAV CCTTC 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 1 cut(s) 190
HpyF3I CTNAG 3 cut(s) 51, 86, 93
HpySE526I ACGT 1 cut(s) 8
Hsp92II CATG 1 cut(s) 297
Kzo9I GATC 1 cut(s) 33
LguI GCTCTTC 1 cut(s) 155
LpnPI CCDG 7 cut(s) 7, 92, 105, 140, 167, 218, 248
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 1 cut(s) 226
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 2 cut(s) 206, 296
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 1 cut(s) 102
MlyI GAGTC 2 cut(s) 76, 293
MnlI CCTC 2 cut(s) 18, 247
MseI TTAA 2 cut(s) 246, 282
MspR9I CCNGG 1 cut(s) 155
MvaI CCWGG 1 cut(s) 155
NdeII GATC 1 cut(s) 33
NlaIII CATG 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 206, 296
NspI RCATGY 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 201
PkrI GCNGC 1 cut(s) 309
Ple19I CGATCG 1 cut(s) 36
PleI GAGTC 2 cut(s) 76, 293
PpsI GAGTC 2 cut(s) 76, 293
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 153
PspGI CCWGG 1 cut(s) 153
PspPI GGNCC 1 cut(s) 219
PstNI CAGNNNCTG 1 cut(s) 204
PvuI CGATCG 1 cut(s) 36
SacI GAGCTC 1 cut(s) 99
SalI GTCGAC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 2 cut(s) 246, 282
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 2 cut(s) 76, 293
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 1 cut(s) 226
SfcI CTRYAG 1 cut(s) 196
SinI GGWCC 1 cut(s) 219
Sse9I AATT 1 cut(s) 102
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 153
TaiI ACGT 1 cut(s) 11
TaqI TCGA 3 cut(s) 29, 123, 147
TasI AATT 1 cut(s) 102
TfiI GAWTC 1 cut(s) 201
Tru1I TTAA 2 cut(s) 246, 282
Tru9I TTAA 2 cut(s) 246, 282
TseFI GTSAC 2 cut(s) 206, 296
TseI GCWGC 1 cut(s) 307
Tsp45I GTSAC 2 cut(s) 206, 296
TspDTI ATGAA 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 219
XceI RCATGY 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.