AT4G25600

ShK toxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015281
Physical Location & Seq
Forward (+)
13060612 .. 13062680
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25600.3

Sequence Viewer

Length: 876 bp
ATGGCTTGCCTTAGCCGAATCTTCCTTATTCTGATGATTACTATGTCTTCTTCGTCTCCTCCTTTCTGTTCCGGTGGAAGTCGGAAGGAGCTCCGAGATAAAGAAATTACGAGTAAGAGTGATGATACACAGGCGAGTTATGTTTTAGGATCTAAATTTGTGGACCCGACACGTGTTCTTCAACTTTCATGGCTACCAAGGGTCTTTCTATACCGCGGGTTCTTGTCGGAGGAAGAGTGTGATCATCTGATATCTCTGAGAAAGGAGACTACAGAGGTTTACTCAGTGGATGCTGATGGAAAGACGCAACTTGATCCAGTAGTTGCTGGGATTGAAGAAAAAGTTTCTGCGTGGACTTTCCTCCCAGGAGAAAATGGCGGCTCTATCAAGGTAAGAAGTTACACTTCTGAGAAGTCAGGAAAGAAGTTGGATTATTTTGGAGAGGAACCTAGCTCCGTATTGCATGAATCTTTGCTGGCAACCGTCGTTCTCTACCTCTCAAACACAACTCAAGGAGGAGAGCTTCTCTTCCCAAATTCAGAGATGAAACCCAAAAACAGTTGCTTGGAGGGCGGTAACATTTTAAGACCAGTAAAAGGAAACGCAATTCTGTTCTTCACCAGGCTCTTGAATGCATCTTTGGATGGGAAAAGCACTCATTTGAGGTGCCCTGTGGTGAAAGGAGAACTGTTAGTGGCAACAAAACTGATCTATGCGAAGAAACAAGCAAGAATTGAGGAGAGTGGGGAATGCAGTGATGAAGACGAAAACTGTGGACGATGGGCTAAGCTTGGGGAGTGCAAGAAAAACCCTGTCTATATGATTGGCTCTCCTGATTACTACGGTACCTGCAGAAAGAGTTGTAACGCTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

291

Amino Acids

32.17

Weight (kDa)

6.83

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 857
Acc65I GGTACC 1 cut(s) 845
AccB1I GGYRCC 2 cut(s) 666, 845
AccII CGCG 1 cut(s) 216
AciI CCGC 4 cut(s) 214, 216, 378, 573
AclWI GGATC 2 cut(s) 157, 308
AcsI RAATTY 2 cut(s) 155, 535
AcvI CACGTG 1 cut(s) 173
AfaI GTAC 1 cut(s) 847
AfiI CCNNNNNNNGG 1 cut(s) 596
AflIII ACRYGT 2 cut(s) 170, 172
AgsI TTSAA 3 cut(s) 182, 335, 631
AjnI CCWGG 2 cut(s) 364, 620
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AluBI AGCT 4 cut(s) 91, 453, 523, 790
AluI AGCT 4 cut(s) 91, 453, 523, 790
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 2 cut(s) 60, 260
AlwI GGATC 2 cut(s) 157, 308
ApoI RAATTY 2 cut(s) 155, 535
Asp718I GGTACC 1 cut(s) 845
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 610, 688
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 671
BaeI ACNNNNGTAYC 2 cut(s) 829, 862
BanI GGYRCC 2 cut(s) 666, 845
BanII GRGCYC 1 cut(s) 93
BbrPI CACGTG 1 cut(s) 173
BbsI GAAGAC 2 cut(s) 39, 768
Bbv12I GWGCWC 1 cut(s) 93
BccI CCATC 3 cut(s) 290, 638, 774
BciT130I CCWGG 2 cut(s) 366, 622
BclI TGATCA 1 cut(s) 241
BcoDI GTCTC 2 cut(s) 60, 260
BfaI CTAG 1 cut(s) 450
BfmI CTRYAG 2 cut(s) 270, 850
BfuAI ACCTGC 1 cut(s) 857
BisI GCNGC 1 cut(s) 379
BlpI GCTNAGC 1 cut(s) 786
BlsI GCNGC 1 cut(s) 380
Bme1390I CCNGG 2 cut(s) 366, 622
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 4 cut(s) 165, 447, 668, 847
BmrFI CCNGG 2 cut(s) 366, 622
BmsI GCATC 2 cut(s) 280, 644
BpiI GAAGAC 2 cut(s) 39, 768
BplI GAGNNNNNCTC 4 cut(s) 266, 298, 510, 542
Bpu10I CCTNAGC 1 cut(s) 11
Bpu1102I GCTNAGC 1 cut(s) 786
BpuEI CTTGAG 1 cut(s) 495
BsaAI YACGTR 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 197, 214, 364
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 1 cut(s) 596
Bse1I ACTGG 2 cut(s) 317, 590
BseBI CCWGG 2 cut(s) 366, 622
BseDI CCNNGG 3 cut(s) 197, 214, 364
BseGI GGATG 2 cut(s) 295, 649
BseLI CCNNNNNNNGG 1 cut(s) 596
BseMII CTCAG 3 cut(s) 248, 297, 399
BseNI ACTGG 2 cut(s) 317, 590
BseRI GAGGAG 3 cut(s) 48, 531, 752
BseSI GKGCMC 1 cut(s) 671
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 216
BshNI GGYRCC 2 cut(s) 666, 845
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 596
BsmAI GTCTC 2 cut(s) 60, 260
BsmBI CGTCTC 1 cut(s) 60
BsmI GAATGC 2 cut(s) 637, 755
Bsp1286I GDGCHC 2 cut(s) 93, 671
Bsp143I GATC 4 cut(s) 149, 241, 313, 708
Bsp1720I GCTNAGC 1 cut(s) 786
BspACI CCGC 4 cut(s) 214, 216, 378, 573
BspCNI CTCAG 3 cut(s) 249, 296, 400
BspFNI CGCG 1 cut(s) 216
BspLI GGNNCC 4 cut(s) 165, 447, 668, 847
BspMAI CTGCAG 1 cut(s) 854
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 2 cut(s) 157, 308
BspT107I GGYRCC 2 cut(s) 666, 845
BsrI ACTGG 2 cut(s) 317, 590
BssECI CCNNGG 3 cut(s) 197, 214, 364
BssMI GATC 4 cut(s) 149, 241, 313, 708
BssT1I CCWWGG 1 cut(s) 197
Bst2UI CCWGG 2 cut(s) 366, 622
Bst4CI ACNGT 5 cut(s) 484, 560, 690, 773, 845
Bst6I CTCTTC 2 cut(s) 228, 533
BstBAI YACGTR 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 7, 477
BstDEI CTNAG 5 cut(s) 11, 257, 283, 408, 786
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 2 cut(s) 295, 649
BstFNI CGCG 1 cut(s) 216
BstKTI GATC 4 cut(s) 152, 244, 316, 711
BstMAI GTCTC 2 cut(s) 60, 260
BstMBI GATC 4 cut(s) 149, 241, 313, 708
BstMWI GCNNNNNNNGC 1 cut(s) 570
BstNI CCWGG 2 cut(s) 366, 622
BstSCI CCNGG 2 cut(s) 364, 620
BstSFI CTRYAG 2 cut(s) 270, 850
BstSLI GKGCMC 1 cut(s) 671
BstUI CGCG 1 cut(s) 216
BstV2I GAAGAC 2 cut(s) 39, 768
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 2 cut(s) 295, 649
BtsI GCAGTG 1 cut(s) 760
BtsIMutI CAGTG 2 cut(s) 291, 760
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 2 cut(s) 7, 477
Cfr13I GGNCC 1 cut(s) 163
Cfr42I CCGCGG 1 cut(s) 217
CseI GACGC 1 cut(s) 313
Csp6I GTAC 1 cut(s) 846
CviAII CATG 2 cut(s) 189, 464
CviQI GTAC 1 cut(s) 846
DdeI CTNAG 5 cut(s) 11, 257, 283, 408, 786
DpnI GATC 4 cut(s) 151, 243, 315, 710
DpnII GATC 4 cut(s) 149, 241, 313, 708
Eam1104I CTCTTC 2 cut(s) 228, 533
EarI CTCTTC 2 cut(s) 228, 533
Ecl136II GAGCTC 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 93
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 163
Eco53kI GAGCTC 1 cut(s) 91
Eco72I CACGTG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 364, 620
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 197
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 197
Esp3I CGTCTC 1 cut(s) 60
FaeI CATG 2 cut(s) 192, 467
FaiI YATR 8 cut(s) 44, 141, 190, 211, 465, 714, 819, 821
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FatI CATG 2 cut(s) 188, 463
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 1 cut(s) 379
FokI GGATG 2 cut(s) 302, 656
FriOI GRGCYC 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 379
FspBI CTAG 1 cut(s) 450
GluI GCNGC 1 cut(s) 379
GsaI CCCAGC 1 cut(s) 330
HapII CCGG 1 cut(s) 72
HgaI GACGC 1 cut(s) 313
Hin1II CATG 2 cut(s) 192, 467
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 2 cut(s) 18, 467
HpaII CCGG 1 cut(s) 72
HphI GGTGA 2 cut(s) 610, 688
Hpy166II GTNNAC 4 cut(s) 163, 280, 354, 776
Hpy188I TCNGA 8 cut(s) 33, 84, 95, 229, 249, 258, 409, 541
Hpy188III TCNNGA 3 cut(s) 417, 628, 833
Hpy8I GTNNAC 4 cut(s) 163, 280, 354, 776
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 1 cut(s) 79
HpyCH4III ACNGT 5 cut(s) 484, 560, 690, 773, 845
HpyCH4IV ACGT 1 cut(s) 172
HpyCH4V TGCA 5 cut(s) 463, 635, 753, 801, 852
HpyF10VI GCNNNNNNNGC 1 cut(s) 570
HpyF3I CTNAG 5 cut(s) 11, 257, 283, 408, 786
HpySE526I ACGT 1 cut(s) 172
Hsp92II CATG 2 cut(s) 192, 467
KpnI GGTACC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 241
KspI CCGCGG 1 cut(s) 217
Kzo9I GATC 4 cut(s) 149, 241, 313, 708
LmnI GCTCC 3 cut(s) 88, 96, 458
LweI GCATC 2 cut(s) 280, 644
MaeI CTAG 1 cut(s) 450
MaeII ACGT 1 cut(s) 172
MaeIII GTNAC 3 cut(s) 398, 575, 863
MalI GATC 4 cut(s) 151, 243, 315, 710
MboI GATC 4 cut(s) 149, 241, 313, 708
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 2 cut(s) 93, 671
MluCI AATT 5 cut(s) 105, 155, 535, 606, 732
MmeI TCCRAC 3 cut(s) 62, 207, 408
Mph1103I ATGCAT 1 cut(s) 637
MseI TTAA 1 cut(s) 584
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 366, 622
Mva1269I GAATGC 2 cut(s) 637, 755
MvaI CCWGG 2 cut(s) 366, 622
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 570
NdeII GATC 4 cut(s) 149, 241, 313, 708
NlaIII CATG 2 cut(s) 192, 467
NlaIV GGNNCC 4 cut(s) 165, 447, 668, 847
NsiI ATGCAT 1 cut(s) 637
PctI GAATGC 2 cut(s) 637, 755
PfeI GAWTC 2 cut(s) 18, 467
PkrI GCNGC 1 cut(s) 380
PmaCI CACGTG 1 cut(s) 173
PmlI CACGTG 1 cut(s) 173
Ppu21I YACGTR 1 cut(s) 173
Psp124BI GAGCTC 1 cut(s) 93
Psp6I CCWGG 2 cut(s) 364, 620
PspCI CACGTG 1 cut(s) 173
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 364, 620
PspN4I GGNNCC 4 cut(s) 165, 447, 668, 847
PspPI GGNCC 1 cut(s) 163
PstI CTGCAG 1 cut(s) 854
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 847
RsaNI GTAC 1 cut(s) 846
SacI GAGCTC 1 cut(s) 93
SacII CCGCGG 1 cut(s) 217
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 1 cut(s) 379
Sau3AI GATC 4 cut(s) 149, 241, 313, 708
Sau96I GGNCC 1 cut(s) 163
ScrFI CCNGG 2 cut(s) 366, 622
SduI GDGCHC 2 cut(s) 93, 671
SfaNI GCATC 2 cut(s) 280, 644
SfcI CTRYAG 2 cut(s) 270, 850
Sfr303I CCGCGG 1 cut(s) 217
SgrBI CCGCGG 1 cut(s) 217
SinI GGWCC 1 cut(s) 163
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 5 cut(s) 105, 155, 535, 606, 732
SsiI CCGC 4 cut(s) 214, 216, 378, 573
SspMI CTAG 1 cut(s) 450
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 2 cut(s) 364, 620
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 5 cut(s) 484, 560, 690, 773, 845
TaiI ACGT 1 cut(s) 175
TasI AATT 5 cut(s) 105, 155, 535, 606, 732
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 2 cut(s) 18, 467
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 2 cut(s) 291, 760
TspDTI ATGAA 4 cut(s) 177, 480, 560, 774
TspGWI ACGGA 1 cut(s) 445
TspRI CASTG 2 cut(s) 291, 760
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 2 cut(s) 155, 535
XspI CTAG 1 cut(s) 450
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.