Rroxscaffold_4G00281070

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015281
Physical Location & Seq
Reverse (-)
3453700 .. 3455667
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281070.1

Sequence Viewer

Length: 993 bp
ATGAAAATCTTCCTGAAATTCTGCTGCCTATTTTCTTCTTCTTGTTCTGAATCTTCAATGGCTTCTTCGCTTCTCTCAATCTTTCTTCTTTTGCCGATCTTCTCCATCTCCTCCTGCTCTGCCGAAACTAGCAGGAAGGAGTTGAGGAGTAAGGAATTCAACCAGGAAGCATTGATACAGTTGGGTCACTCGGTTGATTACAACAGAATTGACCCGTCAAGGGTTGTTCAACTTTCTTGGCGACCAAGGGTGTTTCTGTACAAAGGATTCCTATCTGATGAGGAGTGTGACCACCTTATTTCTTTGGCAAATGGTGGAGAAGAGAAGTCTTCGACAGAATATGATGAATCCGGAAACAGCAACACAAATCGGATGCTTAAAAGTTTAGAACTTCCCTTGAACACGAAGGATGGTATAGTTTCAAAGATTGAGGAAAAGATCTCGGCCTGGACTTTCCTTCCTAAAGAGAACAGCAGAGCTTTACAGGTTTTGCATTACGACCTTGAGGAGGCAGAGAAGAATTACAATTATTTTGGCAACAAATCCACATTGGAACAAAGTGAGCCCTTGTTAGCAACAGTTATTCTTTATCTCTCAAACGTCACTCGTGGTGGTGAGATTCTATTCCCAGAGTCAGAGCTGAAGAGCAATGCCAAGTCTGGTTGTGGAAAGAGTAGCAGTATCTTGAAACCAACCAAAGGAAATGCAATTTTGTTTTTCAATCTCCATCCTAACGCTTCTCCCGACAAGAGCAGTTCCCATGCCCGATGCCCCGTACTCGAAGGCGAAATGTGGTGCGCCACAAAGTTCCTTCATGTGAAAGCCATTCCTCAGGAAAATTCCTTCTCCAATTCTGATAGCAGCGATTGCACTGATGAAGACGATAGCTGTCCCAGGTGGGCAGACATTGGGGAGTGTCAAAGGAACCCTGTATTCATGATTGGCTCCGATGATTACTACGGTACATGCAGGAAGAGTTGTAATGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

330

Amino Acids

36.86

Weight (kDa)

5.33

Isoelectric Point (pI)

53.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 350
AcsI RAATTY 3 cut(s) 17, 155, 838
AcuI CTGAAG 1 cut(s) 662
AfaI GTAC 3 cut(s) 260, 777, 964
AfiI CCNNNNNNNGG 3 cut(s) 220, 508, 698
AgsI TTSAA 7 cut(s) 57, 160, 230, 400, 423, 688, 721
AjnI CCWGG 3 cut(s) 162, 446, 893
AluBI AGCT 3 cut(s) 479, 640, 888
AluI AGCT 3 cut(s) 479, 640, 888
Aor13HI TCCGGA 1 cut(s) 350
AoxI GGCC 1 cut(s) 444
ApeKI GCWGC 2 cut(s) 24, 861
ApoI RAATTY 3 cut(s) 17, 155, 838
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 1 cut(s) 800
AsuHPI GGTGA 1 cut(s) 626
AxyI CCTNAGG 1 cut(s) 831
BanII GRGCYC 1 cut(s) 567
BarI GAAGNNNNNNTAC 2 cut(s) 159, 191
BauI CACGAG 1 cut(s) 606
BbsI GAAGAC 2 cut(s) 321, 885
BbvI GCAGC 2 cut(s) 11, 873
BccI CCATC 3 cut(s) 113, 404, 735
BciT130I CCWGG 3 cut(s) 164, 448, 895
BfaI CTAG 1 cut(s) 129
BglII AGATCT 1 cut(s) 438
BisI GCNGC 2 cut(s) 25, 862
BlsI GCNGC 2 cut(s) 26, 863
Bme1390I CCNGG 3 cut(s) 164, 448, 895
BmiI GGNNCC 2 cut(s) 926, 946
BmrFI CCNGG 3 cut(s) 164, 448, 895
BmsI GCATC 2 cut(s) 363, 758
BpiI GAAGAC 2 cut(s) 321, 885
BpuEI CTTGAG 1 cut(s) 524
BsaBI GATNNNNATC 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 245, 893
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 508, 698
Bse21I CCTNAGG 1 cut(s) 831
Bse3DI GCAATG 1 cut(s) 655
Bse8I GATNNNNATC 1 cut(s) 271
BseAI TCCGGA 1 cut(s) 350
BseBI CCWGG 3 cut(s) 164, 448, 895
BseDI CCNNGG 2 cut(s) 245, 893
BseGI GGATG 3 cut(s) 378, 415, 727
BseJI GATNNNNATC 1 cut(s) 271
BseLI CCNNNNNNNGG 3 cut(s) 220, 508, 698
BseMI GCAATG 1 cut(s) 655
BseMII CTCAG 1 cut(s) 845
BseRI GAGGAG 4 cut(s) 100, 160, 296, 521
BseXI GCAGC 2 cut(s) 11, 873
BshFI GGCC 1 cut(s) 446
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 876
BslI CCNNNNNNNGG 3 cut(s) 220, 508, 698
BsmFI GGGAC 1 cut(s) 876
BsnI GGCC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 567
Bsp13I TCCGGA 1 cut(s) 350
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 2 cut(s) 96, 438
BspANI GGCC 1 cut(s) 446
BspCNI CTCAG 1 cut(s) 844
BspEI TCCGGA 1 cut(s) 350
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 2 cut(s) 926, 946
BspQI GCTCTTC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 655
BsrGI TGTACA 1 cut(s) 258
BssECI CCNNGG 2 cut(s) 245, 893
BssMI GATC 2 cut(s) 96, 438
BssSI CACGAG 1 cut(s) 606
BssT1I CCWWGG 1 cut(s) 245
Bst2BI CACGAG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 164, 448, 895
Bst4CI ACNGT 3 cut(s) 180, 580, 962
Bst6I CTCTTC 3 cut(s) 315, 638, 968
BstAPI GCANNNNNTGC 1 cut(s) 867
BstAUI TGTACA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 988
BstDEI CTNAG 1 cut(s) 831
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 3 cut(s) 378, 415, 727
BstHHI GCGC 1 cut(s) 800
BstKTI GATC 2 cut(s) 99, 441
BstMBI GATC 2 cut(s) 96, 438
BstMWI GCNNNNNNNGC 1 cut(s) 867
BstNI CCWGG 3 cut(s) 164, 448, 895
BstNSI RCATGY 2 cut(s) 969, 990
BstSCI CCNGG 3 cut(s) 162, 446, 893
BstV1I GCAGC 2 cut(s) 11, 873
BstV2I GAAGAC 2 cut(s) 321, 885
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
Bsu36I CCTNAGG 1 cut(s) 831
BsuRI GGCC 1 cut(s) 446
BtsCI GGATG 3 cut(s) 378, 415, 727
BtsIMutI CAGTG 1 cut(s) 870
Cac8I GCNNGC 1 cut(s) 988
CciI TCATGA 1 cut(s) 936
CfoI GCGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 259, 776, 963
CviAII CATG 5 cut(s) 761, 815, 937, 966, 987
CviJI RGCY 8 cut(s) 62, 446, 479, 565, 640, 824, 888, 945
CviKI_1 RGCY 8 cut(s) 62, 446, 479, 565, 640, 824, 888, 945
CviQI GTAC 3 cut(s) 259, 776, 963
DdeI CTNAG 1 cut(s) 831
DpnI GATC 2 cut(s) 98, 440
DpnII GATC 2 cut(s) 96, 438
Eam1104I CTCTTC 3 cut(s) 315, 638, 968
EarI CTCTTC 3 cut(s) 315, 638, 968
Eco130I CCWWGG 1 cut(s) 245
Eco24I GRGCYC 1 cut(s) 567
Eco57I CTGAAG 1 cut(s) 662
Eco81I CCTNAGG 1 cut(s) 831
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoRI GAATTC 1 cut(s) 155
EcoRII CCWGG 3 cut(s) 162, 446, 893
EcoT14I CCWWGG 1 cut(s) 245
EcoT22I ATGCAT 1 cut(s) 988
EcoT38I GRGCYC 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 245
FaeI CATG 5 cut(s) 764, 818, 940, 969, 990
FaiI YATR 7 cut(s) 342, 416, 762, 816, 938, 967, 988
FaqI GGGAC 1 cut(s) 876
FatI CATG 5 cut(s) 760, 814, 936, 965, 986
Fnu4HI GCNGC 2 cut(s) 25, 862
FokI GGATG 3 cut(s) 385, 422, 714
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 2 cut(s) 25, 862
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 799
GluI GCNGC 2 cut(s) 25, 862
HaeIII GGCC 1 cut(s) 446
HapII CCGG 1 cut(s) 351
HhaI GCGC 1 cut(s) 800
Hin1II CATG 5 cut(s) 764, 818, 940, 969, 990
Hin6I GCGC 1 cut(s) 798
HinP1I GCGC 1 cut(s) 798
HinfI GANTC 5 cut(s) 50, 267, 347, 619, 632
HpaII CCGG 1 cut(s) 351
HphI GGTGA 1 cut(s) 626
Hpy188I TCNGA 6 cut(s) 49, 277, 372, 637, 856, 949
Hpy188III TCNNGA 6 cut(s) 13, 351, 685, 743, 833, 937
HpyAV CCTTC 6 cut(s) 130, 400, 467, 776, 821, 853
HpyCH4III ACNGT 3 cut(s) 180, 580, 962
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 5 cut(s) 493, 707, 870, 969, 986
HpyF10VI GCNNNNNNNGC 1 cut(s) 867
HpyF3I CTNAG 1 cut(s) 831
HpySE526I ACGT 1 cut(s) 600
Hsp92II CATG 5 cut(s) 764, 818, 940, 969, 990
HspAI GCGC 1 cut(s) 798
Kpn2I TCCGGA 1 cut(s) 350
Kzo9I GATC 2 cut(s) 96, 438
LguI GCTCTTC 1 cut(s) 638
LmnI GCTCC 1 cut(s) 950
Lsp1109I GCAGC 2 cut(s) 11, 873
LweI GCATC 2 cut(s) 363, 758
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 3 cut(s) 185, 287, 601
MalI GATC 2 cut(s) 98, 440
MboI GATC 2 cut(s) 96, 438
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 8 cut(s) 17, 155, 207, 520, 526, 708, 838, 850
MlyI GAGTC 1 cut(s) 641
MnlI CCTC 7 cut(s) 121, 138, 274, 424, 499, 502, 840
Mph1103I ATGCAT 1 cut(s) 988
MroI TCCGGA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 1 cut(s) 378
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 3 cut(s) 164, 448, 895
MvaI CCWGG 3 cut(s) 164, 448, 895
MwoI GCNNNNNNNGC 1 cut(s) 867
NdeII GATC 2 cut(s) 96, 438
NlaIII CATG 5 cut(s) 764, 818, 940, 969, 990
NlaIV GGNNCC 2 cut(s) 926, 946
NmeAIII GCCGAG 1 cut(s) 422
NmuCI GTSAC 3 cut(s) 185, 287, 601
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 2 cut(s) 969, 990
PaeI GCATGC 1 cut(s) 990
PagI TCATGA 1 cut(s) 936
PciSI GCTCTTC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 4 cut(s) 50, 267, 347, 619
PkrI GCNGC 2 cut(s) 26, 863
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 162, 446, 893
PspGI CCWGG 3 cut(s) 162, 446, 893
PspN4I GGNNCC 2 cut(s) 926, 946
PsuI RGATCY 1 cut(s) 438
RsaI GTAC 3 cut(s) 260, 777, 964
RsaNI GTAC 3 cut(s) 259, 776, 963
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 1 cut(s) 378
SatI GCNGC 2 cut(s) 25, 862
Sau3AI GATC 2 cut(s) 96, 438
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 3 cut(s) 164, 448, 895
SduI GDGCHC 1 cut(s) 567
SetI ASST 8 cut(s) 297, 481, 489, 504, 603, 642, 890, 899
SfaNI GCATC 2 cut(s) 363, 758
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SphI GCATGC 1 cut(s) 990
Sse9I AATT 8 cut(s) 17, 155, 207, 520, 526, 708, 838, 850
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 162, 446, 893
StyI CCWWGG 1 cut(s) 245
TaaI ACNGT 3 cut(s) 180, 580, 962
TaiI ACGT 1 cut(s) 603
TaqI TCGA 2 cut(s) 332, 780
TasI AATT 8 cut(s) 17, 155, 207, 520, 526, 708, 838, 850
TatI WGTACW 1 cut(s) 258
TfiI GAWTC 4 cut(s) 50, 267, 347, 619
Tru1I TTAA 1 cut(s) 378
Tru9I TTAA 1 cut(s) 378
TscAI CASTG 1 cut(s) 877
TseFI GTSAC 3 cut(s) 185, 287, 601
TseI GCWGC 2 cut(s) 24, 861
Tsp45I GTSAC 3 cut(s) 185, 287, 601
TspDTI ATGAA 5 cut(s) 17, 360, 803, 891, 925
TspRI CASTG 1 cut(s) 877
XagI CCTNNNNNAGG 1 cut(s) 506
XapI RAATTY 3 cut(s) 17, 155, 838
XceI RCATGY 2 cut(s) 969, 990
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.