MD07G1273300.v1.1

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015281
Physical Location & Seq
Forward (+)
33926134 .. 33928392
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1273300.v1.1.491

Sequence Viewer

Length: 936 bp
ATGGCCTCCCTTGCCTCGATCTTCCTTCTTCTTTCCGTTACGTCTTCCTTCTTCAGCTCTTCTGCCGAAATTAGCAGGAAGGAACTGAGGACTAATCAAACCATCCAAGAAACTGTCATACATTTTGGCCACTCGGTTCATTCTAACCGAATTGACCCGTCACGCGTTGTCCAATTATCTTGGCAACCAAGGGTGTTTTTGTATAAAGGTTTTCTATCAGATGAGGAGTGTGACCACCTTGTTTCTTTGGCACTCGGGGGAGAAGATAAATCTGTGACCGAATATGATGAGTTAGGAAACACCAACACCATGAGGTTAATTAAAAGCTTAGAAATTCCCTTGGACATGGAGGATGAGGTAGTTTCAAGGATTGAGGCAAGAATTTCAGCTTGGACTTTCCTTCCGAAAGAGAACAGCAGAGCTATACAAGTTTTCCATTTTGGGAACGAGGAGGGTGACAAGAATTTCAACTACTTCGGTAACAAGTCCACATTGGAACAAACTGAGCCTTTATTAGCAACAGTTATTTTGTATCTCTCAAATGTCACTCGTGGTGGTGAGATTCTATTCCCCGAATCAGAGCTGACGAGCAAGGTCCAGTCTGATTGTAGAAGGAGCAGCAGCATCTTGAGACCCGTTAAAGGGAACGCGATTTTGTTTTTCACTCTTCATCCGAATGCATCACCTGACAAGAGCAGTCCACATACCAGATGCCCTGTACTTGAAGGGGAAATGTGGTGTGCCACAAAATTCCTTCATGCTAAAGCCATTGCTGGCGAGAAGATCTCTTCTGATTCCGGGAGCAGTGAATGCACCGATGAAGATGATAACTGTCCCAGGTGGGCTTCGATGGGGGAGTGTCAAAGGAACCCGGTTTTCATGGTTGGTTCCCCTGATTACTACGGGACTTGCAGGAAGAGTTGTAATGTGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

312

Amino Acids

34.76

Weight (kDa)

5.36

Isoelectric Point (pI)

49.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 165, 650
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 333, 381, 463, 749
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 641, 642
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 3 cut(s) 366, 469, 725
AjnI CCWGG 1 cut(s) 836
AjuI GAANNNNNNNTTGG 2 cut(s) 373, 405
AluBI AGCT 5 cut(s) 57, 327, 389, 422, 583
AluI AGCT 5 cut(s) 57, 327, 389, 422, 583
Alw26I GTCTC 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 2 cut(s) 3, 127
ApeKI GCWGC 2 cut(s) 618, 621
ApoI RAATTY 4 cut(s) 333, 381, 463, 749
AspS9I GGNCC 1 cut(s) 595
AsuC2I CCSGG 2 cut(s) 799, 872
AsuHPI GGTGA 3 cut(s) 467, 569, 675
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 1 cut(s) 595
BalI TGGCCA 1 cut(s) 129
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 630, 633
BccI CCATC 2 cut(s) 110, 844
BciT130I CCWGG 1 cut(s) 838
BcnI CCSGG 2 cut(s) 799, 872
BcoDI GTCTC 1 cut(s) 625
BglII AGATCT 1 cut(s) 783
BisI GCNGC 2 cut(s) 619, 622
BlsI GCNGC 2 cut(s) 620, 623
Bme1390I CCNGG 3 cut(s) 799, 838, 872
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 254
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 2 cut(s) 869, 889
BmrFI CCNGG 3 cut(s) 799, 838, 872
BmsI GCATC 3 cut(s) 633, 689, 701
BpiI GAAGAC 1 cut(s) 36
BplI GAGNNNNNCTC 2 cut(s) 770, 802
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 2 cut(s) 799, 872
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 3 cut(s) 188, 339, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 641, 642
Bse1I ACTGG 1 cut(s) 598
Bse3DI GCAATG 1 cut(s) 768
BseBI CCWGG 1 cut(s) 838
BseDI CCNNGG 3 cut(s) 188, 339, 836
BseGI GGATG 3 cut(s) 102, 358, 670
BseLI CCNNNNNNNGG 2 cut(s) 641, 642
BseMI GCAATG 1 cut(s) 768
BseMII CTCAG 2 cut(s) 77, 495
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 2 cut(s) 239, 464
BseXI GCAGC 2 cut(s) 630, 633
Bsh1236I CGCG 2 cut(s) 165, 650
BshFI GGCC 2 cut(s) 5, 129
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 2 cut(s) 798, 872
BslFI GGGAC 2 cut(s) 819, 919
BslI CCNNNNNNNGG 2 cut(s) 641, 642
BsmAI GTCTC 1 cut(s) 625
BsmFI GGGAC 2 cut(s) 819, 919
BsmI GAATGC 2 cut(s) 682, 815
BsnI GGCC 2 cut(s) 5, 129
Bso31I GGTCTC 1 cut(s) 625
BsoBI CYCGRG 1 cut(s) 254
Bsp143I GATC 2 cut(s) 18, 783
BspANI GGCC 2 cut(s) 5, 129
BspCNI CTCAG 2 cut(s) 78, 496
BspFNI CGCG 2 cut(s) 165, 650
BspLI GGNNCC 2 cut(s) 869, 889
BspQI GCTCTTC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 768
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 3 cut(s) 188, 339, 836
BssMI GATC 2 cut(s) 18, 783
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 2 cut(s) 188, 339
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 1 cut(s) 838
Bst4CI ACNGT 3 cut(s) 115, 523, 833
Bst6I CTCTTC 4 cut(s) 64, 672, 793, 911
BstAPI GCANNNNNTGC 1 cut(s) 810
BstC8I GCNNGC 1 cut(s) 775
BstDEI CTNAG 3 cut(s) 86, 328, 504
BstF5I GGATG 3 cut(s) 102, 358, 670
BstFNI CGCG 2 cut(s) 165, 650
BstKTI GATC 2 cut(s) 21, 786
BstMAI GTCTC 1 cut(s) 625
BstMBI GATC 2 cut(s) 18, 783
BstMWI GCNNNNNNNGC 2 cut(s) 11, 810
BstNI CCWGG 1 cut(s) 838
BstSCI CCNGG 3 cut(s) 797, 836, 870
BstUI CGCG 2 cut(s) 165, 650
BstV1I GCAGC 2 cut(s) 630, 633
BstV2I GAAGAC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 783
BstYI RGATCY 1 cut(s) 783
BsuRI GGCC 2 cut(s) 5, 129
BtsCI GGATG 3 cut(s) 102, 358, 670
BtsI GCAGTG 1 cut(s) 811
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 1 cut(s) 775
Cfr13I GGNCC 1 cut(s) 595
Csp6I GTAC 1 cut(s) 719
CviAII CATG 4 cut(s) 310, 346, 758, 880
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 3 cut(s) 86, 328, 504
DpnI GATC 2 cut(s) 20, 785
DpnII GATC 2 cut(s) 18, 783
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 4 cut(s) 64, 672, 793, 911
EarI CTCTTC 4 cut(s) 64, 672, 793, 911
Eco130I CCWWGG 2 cut(s) 188, 339
Eco31I GGTCTC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 836
EcoT14I CCWWGG 2 cut(s) 188, 339
EcoT22I ATGCAT 1 cut(s) 682
ErhI CCWWGG 2 cut(s) 188, 339
FaeI CATG 4 cut(s) 313, 349, 761, 883
FaiI YATR 9 cut(s) 119, 204, 285, 311, 347, 425, 705, 759, 881
FaqI GGGAC 2 cut(s) 819, 919
FatI CATG 4 cut(s) 309, 345, 757, 879
Fnu4HI GCNGC 2 cut(s) 619, 622
FokI GGATG 3 cut(s) 89, 365, 657
Fsp4HI GCNGC 2 cut(s) 619, 622
GluI GCNGC 2 cut(s) 619, 622
HaeIII GGCC 2 cut(s) 5, 129
HapII CCGG 2 cut(s) 798, 872
Hin1II CATG 4 cut(s) 313, 349, 761, 883
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 3 cut(s) 562, 575, 794
HpaII CCGG 2 cut(s) 798, 872
HphI GGTGA 3 cut(s) 467, 569, 675
Hpy166II GTNNAC 2 cut(s) 489, 701
Hpy188I TCNGA 6 cut(s) 220, 405, 580, 604, 675, 793
Hpy188III TCNNGA 1 cut(s) 628
Hpy8I GTNNAC 2 cut(s) 489, 701
HpyAV CCTTC 7 cut(s) 35, 58, 73, 410, 606, 719, 764
HpyCH4III ACNGT 3 cut(s) 115, 523, 833
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 680, 813, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 810
HpyF3I CTNAG 3 cut(s) 86, 328, 504
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 4 cut(s) 313, 349, 761, 883
Kzo9I GATC 2 cut(s) 18, 783
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 2 cut(s) 615, 801
Lsp1109I GCAGC 2 cut(s) 630, 633
LweI GCATC 3 cut(s) 633, 689, 701
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 7 cut(s) 37, 159, 230, 274, 455, 479, 544
MalI GATC 2 cut(s) 20, 785
MboI GATC 2 cut(s) 18, 783
MflI RGATCY 1 cut(s) 783
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 8 cut(s) 69, 150, 173, 318, 333, 381, 463, 749
MluI ACGCGT 1 cut(s) 163
MluNI TGGCCA 1 cut(s) 129
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 682
MscI TGGCCA 1 cut(s) 129
MseI TTAA 3 cut(s) 317, 321, 639
MslI CAYNNNNRTG 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 129
MspI CCGG 2 cut(s) 798, 872
MspR9I CCNGG 3 cut(s) 799, 838, 872
Mva1269I GAATGC 2 cut(s) 682, 815
MvaI CCWGG 1 cut(s) 838
MvnI CGCG 2 cut(s) 165, 650
MwoI GCNNNNNNNGC 2 cut(s) 11, 810
NciI CCSGG 2 cut(s) 799, 872
NdeII GATC 2 cut(s) 18, 783
NlaIII CATG 4 cut(s) 313, 349, 761, 883
NlaIV GGNNCC 2 cut(s) 869, 889
NmuCI GTSAC 5 cut(s) 159, 230, 274, 455, 544
NsiI ATGCAT 1 cut(s) 682
PacI TTAATTAA 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 64
PctI GAATGC 2 cut(s) 682, 815
PfeI GAWTC 3 cut(s) 562, 575, 794
PfoI TCCNGGA 1 cut(s) 797
PkrI GCNGC 2 cut(s) 620, 623
Psp6I CCWGG 1 cut(s) 836
PspGI CCWGG 1 cut(s) 836
PspN4I GGNNCC 2 cut(s) 869, 889
PspPI GGNCC 1 cut(s) 595
PsuI RGATCY 1 cut(s) 783
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 3 cut(s) 317, 321, 639
SatI GCNGC 2 cut(s) 619, 622
Sau3AI GATC 2 cut(s) 18, 783
Sau96I GGNCC 1 cut(s) 595
ScrFI CCNGG 3 cut(s) 799, 838, 872
SfaNI GCATC 3 cut(s) 633, 689, 701
SinI GGWCC 1 cut(s) 595
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 628
SmoI CTYRAG 1 cut(s) 628
Sse9I AATT 8 cut(s) 69, 150, 173, 318, 333, 381, 463, 749
StyD4I CCNGG 3 cut(s) 797, 836, 870
StyI CCWWGG 2 cut(s) 188, 339
TaaI ACNGT 3 cut(s) 115, 523, 833
TaiI ACGT 1 cut(s) 44
TaqI TCGA 2 cut(s) 17, 848
TaqII GACCGA 1 cut(s) 293
TasI AATT 8 cut(s) 69, 150, 173, 318, 333, 381, 463, 749
TatI WGTACW 1 cut(s) 718
TfiI GAWTC 3 cut(s) 562, 575, 794
Tru1I TTAA 3 cut(s) 317, 321, 639
Tru9I TTAA 3 cut(s) 317, 321, 639
TscAI CASTG 1 cut(s) 811
TseFI GTSAC 5 cut(s) 159, 230, 274, 455, 544
TseI GCWGC 2 cut(s) 618, 621
Tsp45I GTSAC 5 cut(s) 159, 230, 274, 455, 544
TspDTI ATGAA 5 cut(s) 128, 659, 746, 834, 868
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 811
VpaK11BI GGWCC 1 cut(s) 595
XapI RAATTY 4 cut(s) 333, 381, 463, 749
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.