pycom07g24990

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015281
Physical Location & Seq
Forward (+)
25460393 .. 25462323
1931 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24990.3

Sequence Viewer

Length: 912 bp
ATGGCCTCCCTTGCCTCGATCTTCCTTCTTCTTTCCGTTACGTCTTCCTTCTTCAGCTCTTCTGCCGAAATTGGGAGGAAGGAACTGAGGAGTAAGGAAACCATCCAAGAAACTGTCATACATTTTGGCCACTCAGTTCATTCTAACCGAATTGACCCGTCACGCGTTGTCCAACTATCTTGGCAACCAAGGTCAGATGAGGAGTGTGACCACCTTGTTTCTTTGGCACTCGGGGGAGAAGATAAATCTGTGACCGAATATGATGAGTTAGGAAACACCAACACCATGAGGCTGCTTAAAAGCTTAGAAATTCCCTTGGTCATGGAGGATGAGGTAGTTTCAAGGATTGAGGCAAGAATTTCAGCTTGGACTTTCCTTCCGAAAGAGAACAGCAGAGCTTTACAAGTTTTCCATTTTGGGAACGAGGAGGGTGACAAGAATTTCAACTACTTCGGTAACAAGTCCACATTGGAACAAACCGAGCCTTTATTAGCAACAGTTGTTTTGTATCTCTCAAATGTCACTCGTGGTGGTGAGATTCTATTCCCCGAATCACAGCTGAGGAGCAAGGTCCGGTCTGATTGTAGAAGGAGCAGCAGCATCTTGAGACCCATTAAAGGGAACGCGATTTTGTTTTTCACTCTTCATCCGAATGCATCACCGGACAAGAGCAGTCCACATACCAGATGCCCTGTACTTGAAGGGGAAATGTGGTGTGCCACAAAATTCCTTCATGCTAAATCCATTGGTGGCGAGAAGATCTCATCCGATTCTGGGAGCAGTGAATGCACCGATGAAGACAGTAACTGTCCCAGGTGGGCTTTGATGGGGGAGTGTCAAAGGAACCCAGTTTTCATGGTTGGTTCCCCTGATTACTACGGGACTTGCAGGAAGAGTTGTAATGTGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

304

Amino Acids

33.82

Weight (kDa)

5.81

Isoelectric Point (pI)

57.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 165, 626
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 4 cut(s) 309, 357, 439, 725
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 1 cut(s) 696
AfiI CCNNNNNNNGG 4 cut(s) 72, 617, 618, 774
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 3 cut(s) 342, 445, 701
AjnI CCWGG 1 cut(s) 812
AjuI GAANNNNNNNTTGG 2 cut(s) 349, 381
AluBI AGCT 5 cut(s) 57, 303, 365, 398, 559
AluI AGCT 5 cut(s) 57, 303, 365, 398, 559
Alw26I GTCTC 1 cut(s) 601
AlwNI CAGNNNCTG 1 cut(s) 807
Ama87I CYCGRG 1 cut(s) 230
AoxI GGCC 2 cut(s) 3, 127
ApeKI GCWGC 3 cut(s) 292, 594, 597
ApoI RAATTY 4 cut(s) 309, 357, 439, 725
AspS9I GGNCC 1 cut(s) 571
AsuHPI GGTGA 3 cut(s) 443, 545, 651
AvaI CYCGRG 1 cut(s) 230
AvaII GGWCC 1 cut(s) 571
BalI TGGCCA 1 cut(s) 129
BauI CACGAG 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 36, 804
BbvCI CCTCAGC 1 cut(s) 560
BbvI GCAGC 3 cut(s) 279, 606, 609
BccI CCATC 2 cut(s) 110, 820
BciT130I CCWGG 1 cut(s) 814
BcoDI GTCTC 1 cut(s) 601
BglII AGATCT 1 cut(s) 759
BisI GCNGC 3 cut(s) 293, 595, 598
BlsI GCNGC 3 cut(s) 294, 596, 599
Bme1390I CCNGG 1 cut(s) 814
Bme18I GGWCC 1 cut(s) 571
BmeT110I CYCGRG 1 cut(s) 230
BmgT120I GGNCC 1 cut(s) 571
BmiI GGNNCC 2 cut(s) 845, 865
BmrFI CCNGG 1 cut(s) 814
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 3 cut(s) 609, 665, 677
BmuI ACTGGG 1 cut(s) 842
BpiI GAAGAC 2 cut(s) 36, 804
BplI GAGNNNNNCTC 2 cut(s) 746, 778
Bpu10I CCTNAGC 1 cut(s) 560
BpuEI CTTGAG 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 601
BsaJI CCNNGG 3 cut(s) 188, 315, 812
BsaWI WCCGGW 2 cut(s) 573, 661
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 617, 618, 774
Bse1I ACTGG 1 cut(s) 848
BseBI CCWGG 1 cut(s) 814
BseDI CCNNGG 3 cut(s) 188, 315, 812
BseGI GGATG 4 cut(s) 102, 334, 646, 764
BseLI CCNNNNNNNGG 4 cut(s) 72, 617, 618, 774
BseMII CTCAG 3 cut(s) 77, 147, 551
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 4 cut(s) 103, 215, 440, 577
BseXI GCAGC 3 cut(s) 279, 606, 609
Bsh1236I CGCG 2 cut(s) 165, 626
BshFI GGCC 2 cut(s) 5, 129
BsiHKCI CYCGRG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 574, 662
BslFI GGGAC 2 cut(s) 795, 895
BslI CCNNNNNNNGG 4 cut(s) 72, 617, 618, 774
BsmAI GTCTC 1 cut(s) 601
BsmFI GGGAC 2 cut(s) 795, 895
BsmI GAATGC 2 cut(s) 658, 791
BsnI GGCC 2 cut(s) 5, 129
Bso31I GGTCTC 1 cut(s) 601
BsoBI CYCGRG 1 cut(s) 230
Bsp143I GATC 2 cut(s) 18, 759
BspANI GGCC 2 cut(s) 5, 129
BspCNI CTCAG 3 cut(s) 78, 146, 552
BspFNI CGCG 2 cut(s) 165, 626
BspLI GGNNCC 2 cut(s) 845, 865
BspQI GCTCTTC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 601
BsrI ACTGG 1 cut(s) 848
BssECI CCNNGG 3 cut(s) 188, 315, 812
BssMI GATC 2 cut(s) 18, 759
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 2 cut(s) 188, 315
Bst2BI CACGAG 1 cut(s) 525
Bst2UI CCWGG 1 cut(s) 814
Bst4CI ACNGT 4 cut(s) 115, 499, 803, 809
Bst6I CTCTTC 3 cut(s) 64, 648, 887
BstAPI GCANNNNNTGC 1 cut(s) 786
BstDEI CTNAG 4 cut(s) 86, 133, 304, 560
BstF5I GGATG 4 cut(s) 102, 334, 646, 764
BstFNI CGCG 2 cut(s) 165, 626
BstKTI GATC 2 cut(s) 21, 762
BstMAI GTCTC 1 cut(s) 601
BstMBI GATC 2 cut(s) 18, 759
BstMWI GCNNNNNNNGC 2 cut(s) 11, 786
BstNI CCWGG 1 cut(s) 814
BstSCI CCNGG 1 cut(s) 812
BstUI CGCG 2 cut(s) 165, 626
BstV1I GCAGC 3 cut(s) 279, 606, 609
BstV2I GAAGAC 2 cut(s) 36, 804
BstX2I RGATCY 1 cut(s) 759
BstYI RGATCY 1 cut(s) 759
BsuRI GGCC 2 cut(s) 5, 129
BtsCI GGATG 4 cut(s) 102, 334, 646, 764
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 1 cut(s) 787
CaiI CAGNNNCTG 1 cut(s) 807
Cfr13I GGNCC 1 cut(s) 571
Csp6I GTAC 1 cut(s) 695
CviAII CATG 4 cut(s) 286, 322, 734, 856
CviQI GTAC 1 cut(s) 695
DdeI CTNAG 4 cut(s) 86, 133, 304, 560
DpnI GATC 2 cut(s) 20, 761
DpnII GATC 2 cut(s) 18, 759
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 64, 648, 887
EarI CTCTTC 3 cut(s) 64, 648, 887
Eco130I CCWWGG 2 cut(s) 188, 315
Eco31I GGTCTC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 571
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 812
EcoT14I CCWWGG 2 cut(s) 188, 315
EcoT22I ATGCAT 1 cut(s) 658
ErhI CCWWGG 2 cut(s) 188, 315
FaeI CATG 4 cut(s) 289, 325, 737, 859
FaiI YATR 7 cut(s) 119, 261, 287, 323, 681, 735, 857
FaqI GGGAC 2 cut(s) 795, 895
FatI CATG 4 cut(s) 285, 321, 733, 855
Fnu4HI GCNGC 3 cut(s) 293, 595, 598
FokI GGATG 4 cut(s) 89, 341, 633, 751
Fsp4HI GCNGC 3 cut(s) 293, 595, 598
GluI GCNGC 3 cut(s) 293, 595, 598
HaeIII GGCC 2 cut(s) 5, 129
HapII CCGG 2 cut(s) 574, 662
Hin1II CATG 4 cut(s) 289, 325, 737, 859
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 3 cut(s) 538, 551, 770
HpaII CCGG 2 cut(s) 574, 662
HphI GGTGA 3 cut(s) 443, 545, 651
Hpy166II GTNNAC 2 cut(s) 465, 677
Hpy188I TCNGA 5 cut(s) 196, 381, 580, 651, 769
Hpy188III TCNNGA 1 cut(s) 604
Hpy8I GTNNAC 2 cut(s) 465, 677
HpyAV CCTTC 7 cut(s) 35, 58, 73, 386, 582, 695, 740
HpyCH4III ACNGT 4 cut(s) 115, 499, 803, 809
HpyCH4IV ACGT 1 cut(s) 41
HpyCH4V TGCA 3 cut(s) 656, 789, 888
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 786
HpyF3I CTNAG 4 cut(s) 86, 133, 304, 560
HpySE526I ACGT 1 cut(s) 41
Hsp92II CATG 4 cut(s) 289, 325, 737, 859
Kzo9I GATC 2 cut(s) 18, 759
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 3 cut(s) 564, 591, 777
Lsp1109I GCAGC 3 cut(s) 279, 606, 609
LweI GCATC 3 cut(s) 609, 665, 677
MaeII ACGT 1 cut(s) 41
MaeIII GTNAC 8 cut(s) 37, 159, 206, 250, 431, 455, 520, 803
MalI GATC 2 cut(s) 20, 761
MboI GATC 2 cut(s) 18, 759
MflI RGATCY 1 cut(s) 759
MlsI TGGCCA 1 cut(s) 129
MluCI AATT 6 cut(s) 69, 150, 309, 357, 439, 725
MluI ACGCGT 1 cut(s) 163
MluNI TGGCCA 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 196
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 658
MscI TGGCCA 1 cut(s) 129
MseI TTAA 2 cut(s) 297, 615
MslI CAYNNNNRTG 1 cut(s) 651
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 559
MspI CCGG 2 cut(s) 574, 662
MspR9I CCNGG 1 cut(s) 814
Mva1269I GAATGC 2 cut(s) 658, 791
MvaI CCWGG 1 cut(s) 814
MvnI CGCG 2 cut(s) 165, 626
MwoI GCNNNNNNNGC 2 cut(s) 11, 786
NdeII GATC 2 cut(s) 18, 759
NlaIII CATG 4 cut(s) 289, 325, 737, 859
NlaIV GGNNCC 2 cut(s) 845, 865
NmuCI GTSAC 5 cut(s) 159, 206, 250, 431, 520
NsiI ATGCAT 1 cut(s) 658
PciSI GCTCTTC 1 cut(s) 64
PctI GAATGC 2 cut(s) 658, 791
PfeI GAWTC 3 cut(s) 538, 551, 770
PkrI GCNGC 3 cut(s) 294, 596, 599
Psp6I CCWGG 1 cut(s) 812
PspGI CCWGG 1 cut(s) 812
PspN4I GGNNCC 2 cut(s) 845, 865
PspPI GGNCC 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 807
PsuI RGATCY 1 cut(s) 759
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 1 cut(s) 696
RsaNI GTAC 1 cut(s) 695
RseI CAYNNNNRTG 1 cut(s) 651
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 2 cut(s) 297, 615
SatI GCNGC 3 cut(s) 293, 595, 598
Sau3AI GATC 2 cut(s) 18, 759
Sau96I GGNCC 1 cut(s) 571
ScrFI CCNGG 1 cut(s) 814
SfaNI GCATC 3 cut(s) 609, 665, 677
SinI GGWCC 1 cut(s) 571
SmiMI CAYNNNNRTG 1 cut(s) 651
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
Sse9I AATT 6 cut(s) 69, 150, 309, 357, 439, 725
StyD4I CCNGG 1 cut(s) 812
StyI CCWWGG 2 cut(s) 188, 315
TaaI ACNGT 4 cut(s) 115, 499, 803, 809
TaiI ACGT 1 cut(s) 44
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 269
TasI AATT 6 cut(s) 69, 150, 309, 357, 439, 725
TatI WGTACW 1 cut(s) 694
TfiI GAWTC 3 cut(s) 538, 551, 770
Tru1I TTAA 2 cut(s) 297, 615
Tru9I TTAA 2 cut(s) 297, 615
TscAI CASTG 1 cut(s) 787
TseFI GTSAC 5 cut(s) 159, 206, 250, 431, 520
TseI GCWGC 3 cut(s) 292, 594, 597
Tsp45I GTSAC 5 cut(s) 159, 206, 250, 431, 520
TspDTI ATGAA 5 cut(s) 128, 635, 722, 810, 844
TspGWI ACGGA 1 cut(s) 25
TspRI CASTG 1 cut(s) 787
VpaK11BI GGWCC 1 cut(s) 571
XapI RAATTY 4 cut(s) 309, 357, 439, 725
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.