Rh1AG427400

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015281
Physical Location & Seq
Reverse (-)
65559321 .. 65561142
1822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG427400.1

Sequence Viewer

Length: 936 bp
ATGGCTTCTTCGCTTCTCTCAATCTTTCTTCTTTTGCCGATCTTCTCCATCTCCTCCTGCTCTGCCGAAACTAGCAGGAAGGAGTTAAGGAGTAAGGAATTCAACCAGGAAGCATTGATACAGTTGGGTCACTCGGTTGATTACAACAGAATTGACCCGTCAAGGGTTGTTCAACTTTCTTGGCGACCAAGGGTGTTTCTGTACAAAGGATTCCTATCTGCTGAGGAGTGTGATCACCTTATTTCTTTGGCAAATGGTGGAGAAGAGAAGTCTTCAACAGAGTATGATGAAGCTGGAAACAGCAACACAAATAGGATGCTTAAAAGTTTAGACCTTCCCTTGAGCATGGGGGATGGTATAGTTTCAAAGATTGAGGAAAAGATCTCGGCCTGGACTTTCCTTCCTAAAGAGAACAGCAGAGCTTTACAGGTTTTGCATTACGACCTTGAGGATGCAGAGAAGAATTACAATTATTTTGGCAACAAATCCACATTGGAACAAAGTGAGCCCTTGTTAGCAACAGTTATTCTTTATCTCTCAAACGTCACTCGTGGTGGTGAGATTCTATTCCCGGAGTCAGAGCTGAAGAGCAATGCCCAGTCTGGTTGTGGAAAGAGTAGCAGTATCTTGAAACCAACCAAAGGAAACGCAATTCTGTTTTTCAATCTCCATCCTAATGGATCTCCCGACAAGAGCAGTGCCCATGCCCGATGCCCCGTACTCGAAGGCGAAATGTGGTGGGCCACAAAGTTCCTTCATGTGAAAGCCATTCCTCAGGAAAATTCCTCCTCCAATTCTGATAGCAGCGATTGCACTGATGAAGACGATAGCTGTCCCAGGTGGGCAGACATTGGGGAGTGTCAAAGGAACCCTGTATTCATGATTGGCTCCGATGATTACTACGGTACATGCAGGAAGAGTTGTAATGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

311

Amino Acids

34.57

Weight (kDa)

5.12

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 688
AcsI RAATTY 2 cut(s) 98, 781
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 3 cut(s) 203, 720, 907
AfiI CCNNNNNNNGG 2 cut(s) 163, 641
AgsI TTSAA 6 cut(s) 103, 173, 276, 366, 631, 664
AjnI CCWGG 3 cut(s) 105, 389, 836
AluBI AGCT 4 cut(s) 293, 422, 583, 831
AluI AGCT 4 cut(s) 293, 422, 583, 831
AlwI GGATC 1 cut(s) 688
AoxI GGCC 2 cut(s) 387, 741
ApeKI GCWGC 1 cut(s) 804
ApoI RAATTY 2 cut(s) 98, 781
AspS9I GGNCC 1 cut(s) 741
AsuC2I CCSGG 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 227, 569
AxyI CCTNAGG 1 cut(s) 774
BaeGI GKGCMC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 510
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 2 cut(s) 264, 828
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 816
BccI CCATC 3 cut(s) 56, 347, 678
BciT130I CCWGG 3 cut(s) 107, 391, 838
BclI TGATCA 1 cut(s) 232
BcnI CCSGG 1 cut(s) 572
BfaI CTAG 1 cut(s) 72
BglII AGATCT 1 cut(s) 381
BisI GCNGC 1 cut(s) 805
BlsI GCNGC 1 cut(s) 806
Bme1390I CCNGG 4 cut(s) 107, 391, 572, 838
BmgT120I GGNCC 1 cut(s) 741
BmiI GGNNCC 2 cut(s) 869, 889
BmrFI CCNGG 4 cut(s) 107, 391, 572, 838
BmrI ACTGGG 1 cut(s) 592
BmsI GCATC 3 cut(s) 306, 442, 701
BmuI ACTGGG 1 cut(s) 592
BpiI GAAGAC 2 cut(s) 264, 828
Bpu10I CCTNAGC 1 cut(s) 222
BpuEI CTTGAG 2 cut(s) 361, 467
BpuMI CCSGG 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 214
BsaJI CCNNGG 2 cut(s) 188, 836
Bsc4I CCNNNNNNNGG 2 cut(s) 163, 641
Bse1I ACTGG 1 cut(s) 598
Bse21I CCTNAGG 1 cut(s) 774
Bse3DI GCAATG 1 cut(s) 598
Bse8I GATNNNNATC 1 cut(s) 214
BseBI CCWGG 3 cut(s) 107, 391, 838
BseDI CCNNGG 2 cut(s) 188, 836
BseGI GGATG 4 cut(s) 321, 358, 457, 670
BseJI GATNNNNATC 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 163, 641
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 2 cut(s) 213, 788
BseNI ACTGG 1 cut(s) 598
BseRI GAGGAG 3 cut(s) 43, 239, 778
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 1 cut(s) 816
BshFI GGCC 2 cut(s) 389, 743
BsiSI CCGG 1 cut(s) 572
BslFI GGGAC 1 cut(s) 819
BslI CCNNNNNNNGG 2 cut(s) 163, 641
BsmFI GGGAC 1 cut(s) 819
BsnI GGCC 2 cut(s) 389, 743
Bsp1286I GDGCHC 2 cut(s) 510, 703
Bsp1407I TGTACA 1 cut(s) 201
Bsp143I GATC 4 cut(s) 39, 232, 381, 680
BspANI GGCC 2 cut(s) 389, 743
BspCNI CTCAG 2 cut(s) 214, 787
BspHI TCATGA 1 cut(s) 879
BspLI GGNNCC 2 cut(s) 869, 889
BspPI GGATC 1 cut(s) 688
BspQI GCTCTTC 1 cut(s) 581
BsrDI GCAATG 1 cut(s) 598
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 1 cut(s) 598
BssECI CCNNGG 2 cut(s) 188, 836
BssMI GATC 4 cut(s) 39, 232, 381, 680
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 3 cut(s) 107, 391, 838
Bst4CI ACNGT 3 cut(s) 123, 523, 905
Bst6I CTCTTC 3 cut(s) 258, 581, 911
BstAPI GCANNNNNTGC 1 cut(s) 810
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 931
BstDEI CTNAG 2 cut(s) 222, 774
BstF5I GGATG 4 cut(s) 321, 358, 457, 670
BstKTI GATC 4 cut(s) 42, 235, 384, 683
BstMBI GATC 4 cut(s) 39, 232, 381, 680
BstMWI GCNNNNNNNGC 1 cut(s) 810
BstNI CCWGG 3 cut(s) 107, 391, 838
BstNSI RCATGY 2 cut(s) 912, 933
BstSCI CCNGG 4 cut(s) 105, 389, 570, 836
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 1 cut(s) 816
BstV2I GAAGAC 2 cut(s) 264, 828
BstX2I RGATCY 2 cut(s) 381, 680
BstXI CCANNNNNNTGG 1 cut(s) 677
BstYI RGATCY 2 cut(s) 381, 680
Bsu36I CCTNAGG 1 cut(s) 774
BsuRI GGCC 2 cut(s) 389, 743
BtsCI GGATG 4 cut(s) 321, 358, 457, 670
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 703, 813
Cac8I GCNNGC 1 cut(s) 931
CciI TCATGA 1 cut(s) 879
Cfr13I GGNCC 1 cut(s) 741
Csp6I GTAC 3 cut(s) 202, 719, 906
CviAII CATG 6 cut(s) 346, 704, 758, 880, 909, 930
CviQI GTAC 3 cut(s) 202, 719, 906
DdeI CTNAG 2 cut(s) 222, 774
DpnI GATC 4 cut(s) 41, 234, 383, 682
DpnII GATC 4 cut(s) 39, 232, 381, 680
Eam1104I CTCTTC 3 cut(s) 258, 581, 911
EarI CTCTTC 3 cut(s) 258, 581, 911
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 510
Eco57I CTGAAG 1 cut(s) 605
Eco81I CCTNAGG 1 cut(s) 774
EcoRI GAATTC 1 cut(s) 98
EcoRII CCWGG 3 cut(s) 105, 389, 836
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 510
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 6 cut(s) 349, 707, 761, 883, 912, 933
FaiI YATR 8 cut(s) 285, 347, 359, 705, 759, 881, 910, 931
FaqI GGGAC 1 cut(s) 819
FatI CATG 6 cut(s) 345, 703, 757, 879, 908, 929
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 1 cut(s) 805
FokI GGATG 4 cut(s) 328, 365, 464, 657
FriOI GRGCYC 1 cut(s) 510
Fsp4HI GCNGC 1 cut(s) 805
FspBI CTAG 1 cut(s) 72
GluI GCNGC 1 cut(s) 805
HaeIII GGCC 2 cut(s) 389, 743
HapII CCGG 1 cut(s) 572
Hin1II CATG 6 cut(s) 349, 707, 761, 883, 912, 933
HinfI GANTC 3 cut(s) 210, 562, 575
HpaII CCGG 1 cut(s) 572
HphI GGTGA 2 cut(s) 227, 569
Hpy188I TCNGA 3 cut(s) 580, 799, 892
Hpy188III TCNNGA 4 cut(s) 628, 686, 776, 880
HpyAV CCTTC 5 cut(s) 73, 344, 410, 719, 764
HpyCH4III ACNGT 3 cut(s) 123, 523, 905
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 5 cut(s) 436, 455, 813, 912, 929
HpyF10VI GCNNNNNNNGC 1 cut(s) 810
HpyF3I CTNAG 2 cut(s) 222, 774
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 6 cut(s) 349, 707, 761, 883, 912, 933
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 4 cut(s) 39, 232, 381, 680
LguI GCTCTTC 1 cut(s) 581
LmnI GCTCC 1 cut(s) 893
Lsp1109I GCAGC 1 cut(s) 816
LweI GCATC 3 cut(s) 306, 442, 701
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 2 cut(s) 128, 544
MalI GATC 4 cut(s) 41, 234, 383, 682
MboI GATC 4 cut(s) 39, 232, 381, 680
MboII GAAGA 8 cut(s) 20, 34, 264, 275, 472, 598, 833, 928
MflI RGATCY 2 cut(s) 381, 680
MhlI GDGCHC 2 cut(s) 510, 703
MluCI AATT 7 cut(s) 98, 150, 463, 469, 651, 781, 793
MlyI GAGTC 1 cut(s) 584
MnlI CCTC 7 cut(s) 64, 217, 367, 442, 783, 796, 799
Mph1103I ATGCAT 1 cut(s) 931
MseI TTAA 2 cut(s) 86, 321
MslI CAYNNNNRTG 1 cut(s) 675
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 4 cut(s) 107, 391, 572, 838
MvaI CCWGG 3 cut(s) 107, 391, 838
MwoI GCNNNNNNNGC 1 cut(s) 810
NciI CCSGG 1 cut(s) 572
NdeII GATC 4 cut(s) 39, 232, 381, 680
NlaIII CATG 6 cut(s) 349, 707, 761, 883, 912, 933
NlaIV GGNNCC 2 cut(s) 869, 889
NmeAIII GCCGAG 1 cut(s) 365
NmuCI GTSAC 2 cut(s) 128, 544
NsiI ATGCAT 1 cut(s) 931
NspI RCATGY 2 cut(s) 912, 933
PaeI GCATGC 1 cut(s) 933
PagI TCATGA 1 cut(s) 879
PciSI GCTCTTC 1 cut(s) 581
PfeI GAWTC 2 cut(s) 210, 562
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 1 cut(s) 806
PleI GAGTC 1 cut(s) 583
PpsI GAGTC 1 cut(s) 583
Psp6I CCWGG 3 cut(s) 105, 389, 836
PspGI CCWGG 3 cut(s) 105, 389, 836
PspN4I GGNNCC 2 cut(s) 869, 889
PspPI GGNCC 1 cut(s) 741
PsuI RGATCY 2 cut(s) 381, 680
RsaI GTAC 3 cut(s) 203, 720, 907
RsaNI GTAC 3 cut(s) 202, 719, 906
RseI CAYNNNNRTG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 581
SaqAI TTAA 2 cut(s) 86, 321
SatI GCNGC 1 cut(s) 805
Sau3AI GATC 4 cut(s) 39, 232, 381, 680
Sau96I GGNCC 1 cut(s) 741
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 4 cut(s) 107, 391, 572, 838
SduI GDGCHC 2 cut(s) 510, 703
SfaNI GCATC 3 cut(s) 306, 442, 701
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 2 cut(s) 340, 446
SmoI CTYRAG 2 cut(s) 340, 446
SphI GCATGC 1 cut(s) 933
Sse9I AATT 7 cut(s) 98, 150, 463, 469, 651, 781, 793
SspMI CTAG 1 cut(s) 72
StyD4I CCNGG 4 cut(s) 105, 389, 570, 836
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 3 cut(s) 123, 523, 905
TaiI ACGT 1 cut(s) 546
TaqI TCGA 1 cut(s) 723
TasI AATT 7 cut(s) 98, 150, 463, 469, 651, 781, 793
TatI WGTACW 1 cut(s) 201
TfiI GAWTC 2 cut(s) 210, 562
Tru1I TTAA 2 cut(s) 86, 321
Tru9I TTAA 2 cut(s) 86, 321
TscAI CASTG 2 cut(s) 703, 820
TseFI GTSAC 2 cut(s) 128, 544
TseI GCWGC 1 cut(s) 804
Tsp45I GTSAC 2 cut(s) 128, 544
TspDTI ATGAA 4 cut(s) 303, 746, 834, 868
TspRI CASTG 2 cut(s) 703, 820
XapI RAATTY 2 cut(s) 98, 781
XceI RCATGY 2 cut(s) 912, 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
XspI CTAG 1 cut(s) 72
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.