RLG00000026500

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015281
Physical Location & Seq
Reverse (-)
3419517 .. 3421451
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026500

Sequence Viewer

Length: 939 bp
ATGGCTTCTTCGCTTCTCTCAATCTTTCTTCTTTTGCCGATCTTCTCCTCCATCTCCTCCTGCTCTGCCGAAACTAGCAGGAAGGAGTTAAGGAGTAAGGAATTCAACCAGGAAGCATTGATACAGTTGGGTCACTCGGTTGATTACAACAGAATTGACCCGTCAAGGGTTGTTCAACTTTCTTGGCGACCAAGGGTGTTTCTGTACAAAGGATTCCTATCTGATGAGGAGTGTGATCACCTTATTTCTTTGGCAAATGGTGGAGAAGAGAAGTCTTCAACAGAATATTATGAAGCTGGAAACAGCAACACAAATAGGATGCTTAAAAGTTTTGACCTTCCCTTGAGCACGGAGGATGGTATAGTTTCAAAGATTGAGGAAAAGATATCGGCCTGGACTTTCCTTCCTAAAGAGAACAGCAGAGCTTTACAGGTTTTGCATTACGACCTTGAGGAGGCAGAGAAGAATTACAATTATTTTGGCAACAAATCCACATTGGAACAAAGTGAGCCCTTGTTAGCAACAGTTATTCTTTATCTCTCAAACGTCACTCGTGGTGGTGAGATTCTATTCCCGGAGTCAGAGCTGAAGAGCAATGCCCAGTCTGGTTGTGGAAAGAGTAGCAGTATCTTGAAACCAACCAAAGGAAATGCAATTCTGTTTTTCAATCTCCATCCTAACGCTTCTCCCGACAAGAGCAGTTCCCATGCCCGATGCCCCATACTCGAAGGCGAAATGTGGTGCGCGACAAAGTTCCTTCATGTGAAAGCCATTCCTCAGGAAAATTCCTCCTCCAATTCTGATAGCAGCGATTGCACTGATGAAGACGATAGCTGTCCCAGGTGGGCAGACATTGGGGAGTGTCAAAGGAACCCTGTATTCATGATTGGCTCCGATGATTACTACGGTACATGCAGGAAGAGTTGTAATGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

313

Amino Acids

34.8

Weight (kDa)

5.07

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015281)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25600 AT4G25600 AT4G25600 AT4G25600
fragaria_vesca FvH4_7g29771 FvH4_7g29771
malus_domestica MD07G1273300.v1.1
prunus_persica Prupe.2G297000_v2.0.a1
pyrus_communis pycom07g24990
rosa_chinensis RchiOBHm_Chr1g0377771
rosa_laevigata RLG00000026500
rosa_multiflora Rmu_sc0005758.1_g000003
rosa_roxburghii Rroxscaffold_4G00281070
rosa_rugosa Rorug01G0404900
rosa_samantha Rh1AG427400
rosa_wichuraiana Rw1G037110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 746
AcsI RAATTY 2 cut(s) 101, 784
AcuI CTGAAG 1 cut(s) 608
AfaI GTAC 2 cut(s) 206, 910
AfiI CCNNNNNNNGG 3 cut(s) 166, 454, 644
AgsI TTSAA 6 cut(s) 106, 176, 279, 369, 634, 667
AjnI CCWGG 3 cut(s) 108, 392, 839
AluBI AGCT 4 cut(s) 296, 425, 586, 834
AluI AGCT 4 cut(s) 296, 425, 586, 834
Alw21I GWGCWC 1 cut(s) 350
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 1 cut(s) 807
ApoI RAATTY 2 cut(s) 101, 784
AspLEI GCGC 1 cut(s) 746
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 230, 572
AxyI CCTNAGG 1 cut(s) 777
BanII GRGCYC 1 cut(s) 513
BarI GAAGNNNNNNTAC 2 cut(s) 105, 137
BauI CACGAG 1 cut(s) 552
BbsI GAAGAC 2 cut(s) 267, 831
Bbv12I GWGCWC 1 cut(s) 350
BbvI GCAGC 1 cut(s) 819
BccI CCATC 3 cut(s) 59, 350, 681
BciT130I CCWGG 3 cut(s) 110, 394, 841
BclI TGATCA 1 cut(s) 235
BcnI CCSGG 1 cut(s) 575
BfaI CTAG 1 cut(s) 75
BisI GCNGC 1 cut(s) 808
BlsI GCNGC 1 cut(s) 809
Bme1390I CCNGG 4 cut(s) 110, 394, 575, 841
BmiI GGNNCC 2 cut(s) 872, 892
BmrFI CCNGG 4 cut(s) 110, 394, 575, 841
BmrI ACTGGG 1 cut(s) 595
BmsI GCATC 2 cut(s) 309, 704
BmuI ACTGGG 1 cut(s) 595
BpiI GAAGAC 2 cut(s) 267, 831
BpuEI CTTGAG 2 cut(s) 364, 470
BpuMI CCSGG 1 cut(s) 575
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 191, 839
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 454, 644
Bse1I ACTGG 1 cut(s) 601
Bse21I CCTNAGG 1 cut(s) 777
Bse3DI GCAATG 1 cut(s) 601
Bse8I GATNNNNATC 1 cut(s) 217
BseBI CCWGG 3 cut(s) 110, 394, 841
BseDI CCNNGG 2 cut(s) 191, 839
BseGI GGATG 3 cut(s) 324, 361, 673
BseJI GATNNNNATC 1 cut(s) 217
BseLI CCNNNNNNNGG 3 cut(s) 166, 454, 644
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 1 cut(s) 791
BseNI ACTGG 1 cut(s) 601
BseRI GAGGAG 5 cut(s) 37, 46, 242, 467, 781
BseXI GCAGC 1 cut(s) 819
Bsh1236I CGCG 1 cut(s) 746
BshFI GGCC 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 1 cut(s) 575
BslFI GGGAC 1 cut(s) 822
BslI CCNNNNNNNGG 3 cut(s) 166, 454, 644
BsmFI GGGAC 1 cut(s) 822
BsnI GGCC 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 350, 513
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 39, 235
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 1 cut(s) 790
BspFNI CGCG 1 cut(s) 746
BspHI TCATGA 1 cut(s) 882
BspLI GGNNCC 2 cut(s) 872, 892
BspQI GCTCTTC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 601
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 191, 839
BssMI GATC 2 cut(s) 39, 235
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 191
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 3 cut(s) 110, 394, 841
Bst4CI ACNGT 3 cut(s) 126, 526, 908
Bst6I CTCTTC 3 cut(s) 261, 584, 914
BstAPI GCANNNNNTGC 1 cut(s) 813
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 934
BstDEI CTNAG 1 cut(s) 777
BstENI CCTNNNNNAGG 1 cut(s) 452
BstF5I GGATG 3 cut(s) 324, 361, 673
BstFNI CGCG 1 cut(s) 746
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 2 cut(s) 42, 238
BstMBI GATC 2 cut(s) 39, 235
BstMWI GCNNNNNNNGC 1 cut(s) 813
BstNI CCWGG 3 cut(s) 110, 394, 841
BstNSI RCATGY 2 cut(s) 915, 936
BstSCI CCNGG 4 cut(s) 108, 392, 573, 839
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 1 cut(s) 819
BstV2I GAAGAC 2 cut(s) 267, 831
Bsu36I CCTNAGG 1 cut(s) 777
BsuRI GGCC 1 cut(s) 392
BtsCI GGATG 3 cut(s) 324, 361, 673
BtsIMutI CAGTG 1 cut(s) 816
Cac8I GCNNGC 1 cut(s) 934
CciI TCATGA 1 cut(s) 882
CfoI GCGC 1 cut(s) 746
Csp6I GTAC 2 cut(s) 205, 909
CviAII CATG 5 cut(s) 707, 761, 883, 912, 933
CviJI RGCY 9 cut(s) 5, 296, 392, 425, 511, 586, 770, 834, 891
CviKI_1 RGCY 9 cut(s) 5, 296, 392, 425, 511, 586, 770, 834, 891
CviQI GTAC 2 cut(s) 205, 909
DdeI CTNAG 1 cut(s) 777
DpnI GATC 2 cut(s) 41, 237
DpnII GATC 2 cut(s) 39, 235
Eam1104I CTCTTC 3 cut(s) 261, 584, 914
EarI CTCTTC 3 cut(s) 261, 584, 914
Eco130I CCWWGG 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 513
Eco32I GATATC 1 cut(s) 387
Eco57I CTGAAG 1 cut(s) 608
Eco81I CCTNAGG 1 cut(s) 777
EcoNI CCTNNNNNAGG 1 cut(s) 452
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 3 cut(s) 108, 392, 839
EcoRV GATATC 1 cut(s) 387
EcoT14I CCWWGG 1 cut(s) 191
EcoT22I ATGCAT 1 cut(s) 934
EcoT38I GRGCYC 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 5 cut(s) 710, 764, 886, 915, 936
FaiI YATR 8 cut(s) 291, 362, 708, 722, 762, 884, 913, 934
FaqI GGGAC 1 cut(s) 822
FatI CATG 5 cut(s) 706, 760, 882, 911, 932
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 808
FokI GGATG 3 cut(s) 331, 368, 660
FriOI GRGCYC 1 cut(s) 513
Fsp4HI GCNGC 1 cut(s) 808
FspBI CTAG 1 cut(s) 75
GlaI GCGC 1 cut(s) 745
GluI GCNGC 1 cut(s) 808
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 575
HhaI GCGC 1 cut(s) 746
Hin1II CATG 5 cut(s) 710, 764, 886, 915, 936
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HinfI GANTC 3 cut(s) 213, 565, 578
HpaII CCGG 1 cut(s) 575
HphI GGTGA 2 cut(s) 230, 572
Hpy188I TCNGA 4 cut(s) 223, 583, 802, 895
Hpy188III TCNNGA 4 cut(s) 631, 689, 779, 883
HpyAV CCTTC 5 cut(s) 76, 347, 413, 722, 767
HpyCH4III ACNGT 3 cut(s) 126, 526, 908
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 5 cut(s) 439, 653, 816, 915, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 813
HpyF3I CTNAG 1 cut(s) 777
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 5 cut(s) 710, 764, 886, 915, 936
HspAI GCGC 1 cut(s) 744
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 2 cut(s) 39, 235
LguI GCTCTTC 1 cut(s) 584
LmnI GCTCC 1 cut(s) 896
Lsp1109I GCAGC 1 cut(s) 819
LweI GCATC 2 cut(s) 309, 704
MaeI CTAG 1 cut(s) 75
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 2 cut(s) 131, 547
MalI GATC 2 cut(s) 41, 237
MboI GATC 2 cut(s) 39, 235
MboII GAAGA 8 cut(s) 20, 34, 267, 278, 475, 601, 836, 931
MhlI GDGCHC 2 cut(s) 350, 513
MluCI AATT 7 cut(s) 101, 153, 466, 472, 654, 784, 796
MlyI GAGTC 1 cut(s) 587
Mph1103I ATGCAT 1 cut(s) 934
MseI TTAA 2 cut(s) 89, 324
MspI CCGG 1 cut(s) 575
MspR9I CCNGG 4 cut(s) 110, 394, 575, 841
MvaI CCWGG 3 cut(s) 110, 394, 841
MvnI CGCG 1 cut(s) 746
MwoI GCNNNNNNNGC 1 cut(s) 813
NciI CCSGG 1 cut(s) 575
NdeII GATC 2 cut(s) 39, 235
NlaIII CATG 5 cut(s) 710, 764, 886, 915, 936
NlaIV GGNNCC 2 cut(s) 872, 892
NmuCI GTSAC 2 cut(s) 131, 547
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 2 cut(s) 915, 936
PaeI GCATGC 1 cut(s) 936
PagI TCATGA 1 cut(s) 882
PciSI GCTCTTC 1 cut(s) 584
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PfeI GAWTC 2 cut(s) 213, 565
PfoI TCCNGGA 1 cut(s) 573
PkrI GCNGC 1 cut(s) 809
PleI GAGTC 1 cut(s) 586
PpsI GAGTC 1 cut(s) 586
Psp6I CCWGG 3 cut(s) 108, 392, 839
PspGI CCWGG 3 cut(s) 108, 392, 839
PspN4I GGNNCC 2 cut(s) 872, 892
RsaI GTAC 2 cut(s) 206, 910
RsaNI GTAC 2 cut(s) 205, 909
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 2 cut(s) 89, 324
SatI GCNGC 1 cut(s) 808
Sau3AI GATC 2 cut(s) 39, 235
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 4 cut(s) 110, 394, 575, 841
SduI GDGCHC 2 cut(s) 350, 513
SfaNI GCATC 2 cut(s) 309, 704
SmlI CTYRAG 2 cut(s) 343, 449
SmoI CTYRAG 2 cut(s) 343, 449
SphI GCATGC 1 cut(s) 936
Sse9I AATT 7 cut(s) 101, 153, 466, 472, 654, 784, 796
SspI AATATT 1 cut(s) 287
SspMI CTAG 1 cut(s) 75
StyD4I CCNGG 4 cut(s) 108, 392, 573, 839
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 3 cut(s) 126, 526, 908
TaiI ACGT 1 cut(s) 549
TaqI TCGA 1 cut(s) 726
TasI AATT 7 cut(s) 101, 153, 466, 472, 654, 784, 796
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 2 cut(s) 213, 565
Tru1I TTAA 2 cut(s) 89, 324
Tru9I TTAA 2 cut(s) 89, 324
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 2 cut(s) 131, 547
TseI GCWGC 1 cut(s) 807
Tsp45I GTSAC 2 cut(s) 131, 547
TspDTI ATGAA 4 cut(s) 306, 749, 837, 871
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 823
XagI CCTNNNNNAGG 1 cut(s) 452
XapI RAATTY 2 cut(s) 101, 784
XceI RCATGY 2 cut(s) 915, 936
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XspI CTAG 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.