AT4G27340

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011880
Physical Location & Seq
Reverse (-)
13687174 .. 13690538
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27340.3

Sequence Viewer

Length: 1860 bp
ATGGTGTCAAAGCTTTCTCTTTTCCGAGCCAATTCACTTCCTTTTCCAGTCATCTCTTCCTACTCTGCTCGTTTTATCCTTAAACCCTATCCCAAACCTAAAACCCTTATCTTCTGTGTCTTCTCCAGTAATCTCTCCTCCACCACCTTTCCGTATGGCCCGTCTCTTCTGAAAGGAAAGAAGCCTGTGCTAGATGATATTAGATTAGCTTCAATTGGTCGAGACAGAGATGCCCATCGAGGCAAAATTGGAGAGTTTGATGAATCAATTGAGAAAGATGTTCTTTTGAATGAAGATGAATTTACTAGGGTTTTTGAGATTTCGGCGATTCGAGTCCCGGCGAAGGATTGTTTCGCGCTCGAGAATCGTCTCCGTGGTCATCTTCTTAATTGGCCTAGGATTCGGAACATTGCTAGGGTTCCTGGAGATGAGATTGAAGAAGATGTGGTGAAGCTGTTGGGCCGTGAGACTGATGAAGAAGAAGAAGATGAAGATAGTGTTGTGGATTCGGTTAACCGGAGAATAAGAGGGAAAGCAGAAGGGGATGGGGAGAGATTGAGTTCAGTGTTGCATAGAGATAAACTCGCCAGGACATTTAATTCAACTGGGTATCTCAAATTCAGAAACTTGGCCAAGATTTCTCGGCCAAAGAGGAAGAGGAAGACAGAGAGAACAAGGGAGGGGAAGGAAAAGGAAATAGCAAGCAGACGCAACGAAATGGCGGTGGTGGAAGTGGTGGAAACTCGAGGAGGAGAAGAGGATTTTGAGGGGTTATTGGGAGAAGGCTATGGTAGTAGAGGAAGATGGAGAGGTTCAACAAGATTGTTGCTTTTGGATGAGAAATATTCCGGTGAGGAAGTTCAAGACTTGCCTGAGGCTATCAAGGTTCTGTTTGCAGAAGCTAAAATGGCTGATGCAAGCTTAAGTTTCGAGCTAGTGAAATGCAGACTGACTTTGTTCTATGACTATTGGCCAATGATTGAGATATTGGAGGCTGTACTACCTAAGGGTATGATTGTGCCTTCGGCATTTGAGATGGTGGGGCATATTGCGCATCTGAACCTAAGAGATGAGCATCTACCATACAAAAGGCTCATAGCTAAGGTAGTCCTGGACAAGAATCAGCCAAAGATACAAACCGTTGTGAATAAGATTGATCCTATCCACAATGACTTCAGAACTATGCAGCTAGAGGTTTTAGCTGGAAACCATTCGCTGGTGACTCTAGTTGTTGAAAATGGACTGCGCTTTCATGTTGATTTAGCTAGAGTATATTGGAATTCGAAACTTGGGACAGAAAGACAGAGGCTGCTACTCGGGTTCGACCAAAATGACGTTGTCTGTGATGTTTTTGCTGGAGTTGGTCCTATCGCACTTGCTGCAGCAAGAATTGTAAAACGTGTATATGCCAATGACCTGAATCCTCATGCAGTAGAATTTATGGAACAGAATAGTGTTGTCAATAAGCTCGAGAAGAGAATTGAGATCTTTAACATGGACGGACGAAGATTCATAAAGGCTATGTTTTCAAGCGAAAAAGGTCAAAAAGTCACCCAAGTAGTCATGAATTTACCAAAAGATGCTGCTGAATCTCTAGATGCATTTCGCGGAGTATACAACGATAGGCACAGAGATGAAGGCTTATCTTTCCCAACCATCCATGTGTATGGATTCTCCAAGGCTTCTGATCCAGAATTCGACTTCCACGAGCGGATACGGATTGCTTTATCTGAGGTAGCCGTGGATGTGAAGATGCGAAAGGTACGCCTTGTGGCTCCAGGGAAATGGATGCTATGTGCTTCATTCATACTTCCCAAGAATGTAGCCTTCTCAAGAAAAAACTTAAGTTATGTAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_10

Protein Analysis

619

Amino Acids

70.57

Weight (kDa)

8.86

Isoelectric Point (pI)

37.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW2_N_2 PF25133 328 - 404 1.2e-21 TYW2 N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27340 AT4G27340 AT4G27340
fragaria_vesca FvH4_3g21210
malus_domestica MD03G1217000.v1.1
prunus_persica Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1
pyrus_communis pycom03g16620
rosa_chinensis RchiOBHm_Chr5g0036281
rosa_laevigata RLG00000033683
rosa_multiflora Rmu_sc0000247.1_g000072
rosa_roxburghii Rroxscaffold_1G00044510
rosa_rugosa Rorug05G0154500
rosa_samantha Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500
rosa_wichuraiana Rw5G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1053
AccB7I CCANNNNNTGG 1 cut(s) 1216
AccBSI CCGCTC 1 cut(s) 1711
AccI GTMKAC 1 cut(s) 1614
AccII CGCG 2 cut(s) 356, 1608
AciI CCGC 3 cut(s) 722, 1608, 1711
AclWI GGATC 2 cut(s) 1151, 1682
AcoI YGGCCR 3 cut(s) 630, 644, 971
AcsI RAATTY 6 cut(s) 299, 617, 1279, 1436, 1567, 1694
AcuI CTGAAG 1 cut(s) 1159
AfaI GTAC 2 cut(s) 999, 1764
AfiI CCNNNNNNNGG 2 cut(s) 343, 1216
AflII CTTAAG 2 cut(s) 922, 1843
AflIII ACRYGT 1 cut(s) 1399
AgsI TTSAA 8 cut(s) 213, 289, 437, 603, 816, 863, 1235, 1530
AjnI CCWGG 4 cut(s) 421, 587, 1110, 1777
Alw26I GTCTC 4 cut(s) 168, 216, 374, 461
AlwI GGATC 2 cut(s) 1151, 1682
AlwNI CAGNNNCTG 1 cut(s) 1309
Ama87I CYCGRG 4 cut(s) 359, 744, 1316, 1469
AoxI GGCC 6 cut(s) 157, 392, 460, 630, 644, 971
ApeKI GCWGC 5 cut(s) 1186, 1309, 1379, 1382, 1583
ApoI RAATTY 6 cut(s) 299, 617, 1279, 1436, 1567, 1694
Asp700I GAANNNNTTC 1 cut(s) 1210
AspA2I CCTAGG 1 cut(s) 395
AspLEI GCGC 3 cut(s) 358, 1054, 1248
AspS9I GGNCC 3 cut(s) 158, 460, 1364
AsuC2I CCSGG 1 cut(s) 338
AsuHPI GGTGA 4 cut(s) 460, 863, 1231, 1543
AsuII TTCGAA 1 cut(s) 1283
AvaI CYCGRG 4 cut(s) 359, 744, 1316, 1469
AvaII GGWCC 1 cut(s) 1364
AvrII CCTAGG 1 cut(s) 395
AxyI CCTNAGG 2 cut(s) 873, 1005
BalI TGGCCA 2 cut(s) 632, 973
BauI CACGAG 1 cut(s) 1706
BbsI GAAGAC 2 cut(s) 112, 668
BbvI GCAGC 5 cut(s) 1198, 1296, 1366, 1394, 1570
BccI CCATC 5 cut(s) 243, 539, 798, 1030, 1664
BceAI ACGGC 2 cut(s) 447, 1724
BciT130I CCWGG 4 cut(s) 423, 589, 1112, 1779
BciVI GTATCC 1 cut(s) 1707
BcnI CCSGG 1 cut(s) 338
BcoDI GTCTC 4 cut(s) 168, 216, 374, 461
BfaI CTAG 9 cut(s) 191, 306, 396, 414, 935, 1190, 1226, 1266, 1595
BfmI CTRYAG 1 cut(s) 1380
BfrI CTTAAG 2 cut(s) 922, 1843
BfuI GTATCC 1 cut(s) 1707
BglII AGATCT 1 cut(s) 1485
BisI GCNGC 5 cut(s) 1187, 1310, 1380, 1383, 1584
BlnI CCTAGG 1 cut(s) 395
BlsI GCNGC 5 cut(s) 1188, 1311, 1381, 1384, 1585
Bme1390I CCNGG 5 cut(s) 338, 423, 589, 1112, 1779
Bme18I GGWCC 1 cut(s) 1364
BmeT110I CYCGRG 4 cut(s) 359, 744, 1316, 1469
BmgT120I GGNCC 3 cut(s) 158, 460, 1364
BmiI GGNNCC 2 cut(s) 420, 1776
BmrFI CCNGG 5 cut(s) 338, 423, 589, 1112, 1779
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 8 cut(s) 220, 904, 1063, 1084, 1570, 1588, 1743, 1779
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 2 cut(s) 112, 668
BplI GAGNNNNNCTC 2 cut(s) 567, 599
BpmI CTGGAG 4 cut(s) 109, 444, 1377, 1761
Bpu10I CCTNAGC 1 cut(s) 1101
Bpu14I TTCGAA 1 cut(s) 1283
BpuEI CTTGAG 1 cut(s) 1816
BpuMI CCSGG 1 cut(s) 338
BsaBI GATNNNNATC 2 cut(s) 234, 1074
BsaJI CCNNGG 5 cut(s) 373, 395, 1677, 1740, 1778
BsaWI WCCGGW 2 cut(s) 516, 848
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 1216
Bse1I ACTGG 3 cut(s) 47, 126, 610
Bse21I CCTNAGG 2 cut(s) 873, 1005
Bse3DI GCAATG 1 cut(s) 408
Bse8I GATNNNNATC 2 cut(s) 234, 1074
BseBI CCWGG 4 cut(s) 423, 589, 1112, 1779
BseDI CCNNGG 5 cut(s) 373, 395, 1677, 1740, 1778
BseGI GGATG 5 cut(s) 550, 841, 1656, 1750, 1794
BseJI GATNNNNATC 2 cut(s) 234, 1074
BseLI CCNNNNNNNGG 2 cut(s) 343, 1216
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 864, 1722
BseNI ACTGG 3 cut(s) 47, 126, 610
BseRI GAGGAG 3 cut(s) 127, 762, 765
BseXI GCAGC 5 cut(s) 1198, 1296, 1366, 1394, 1570
Bsh1236I CGCG 2 cut(s) 356, 1608
BshFI GGCC 6 cut(s) 159, 394, 462, 632, 646, 973
BsiHKCI CYCGRG 4 cut(s) 359, 744, 1316, 1469
BsiSI CCGG 3 cut(s) 338, 517, 849
BslFI GGGAC 2 cut(s) 320, 1306
BslI CCNNNNNNNGG 2 cut(s) 343, 1216
BsmAI GTCTC 4 cut(s) 168, 216, 374, 461
BsmBI CGTCTC 2 cut(s) 168, 374
BsmFI GGGAC 2 cut(s) 320, 1306
BsnI GGCC 6 cut(s) 159, 394, 462, 632, 646, 973
BsoBI CYCGRG 4 cut(s) 359, 744, 1316, 1469
Bsp119I TTCGAA 1 cut(s) 1283
Bsp143I GATC 3 cut(s) 1156, 1485, 1687
BspACI CCGC 3 cut(s) 722, 1608, 1711
BspANI GGCC 6 cut(s) 159, 394, 462, 632, 646, 973
BspCNI CTCAG 2 cut(s) 865, 1723
BspFNI CGCG 2 cut(s) 356, 1608
BspHI TCATGA 1 cut(s) 1563
BspLI GGNNCC 2 cut(s) 420, 1776
BspMAI CTGCAG 1 cut(s) 1384
BspPI GGATC 2 cut(s) 1151, 1682
BspT104I TTCGAA 1 cut(s) 1283
BspTI CTTAAG 2 cut(s) 922, 1843
BsrBI CCGCTC 1 cut(s) 1711
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 47, 126, 610
BssECI CCNNGG 5 cut(s) 373, 395, 1677, 1740, 1778
BssMI GATC 3 cut(s) 1156, 1485, 1687
BssNAI GTATAC 1 cut(s) 1615
BssSI CACGAG 1 cut(s) 1706
BssT1I CCWWGG 2 cut(s) 395, 1677
Bst1107I GTATAC 1 cut(s) 1615
Bst2BI CACGAG 1 cut(s) 1706
Bst2UI CCWGG 4 cut(s) 423, 589, 1112, 1779
Bst4CI ACNGT 1 cut(s) 1141
Bst6I CTCTTC 5 cut(s) 61, 171, 650, 750, 1469
BstAFI CTTAAG 2 cut(s) 922, 1843
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstBI TTCGAA 1 cut(s) 1283
BstC8I GCNNGC 2 cut(s) 703, 919
BstDEI CTNAG 5 cut(s) 873, 1005, 1064, 1101, 1731
BstDSI CCRYGG 2 cut(s) 373, 1740
BstF5I GGATG 5 cut(s) 550, 841, 1656, 1750, 1794
BstFNI CGCG 2 cut(s) 356, 1608
BstHHI GCGC 3 cut(s) 358, 1054, 1248
BstKTI GATC 3 cut(s) 1159, 1488, 1690
BstMAI GTCTC 4 cut(s) 168, 216, 374, 461
BstMBI GATC 3 cut(s) 1156, 1485, 1687
BstMWI GCNNNNNNNGC 3 cut(s) 908, 1051, 1379
BstNI CCWGG 4 cut(s) 423, 589, 1112, 1779
BstSCI CCNGG 5 cut(s) 336, 421, 587, 1110, 1777
BstSFI CTRYAG 1 cut(s) 1380
BstUI CGCG 2 cut(s) 356, 1608
BstV1I GCAGC 5 cut(s) 1198, 1296, 1366, 1394, 1570
BstV2I GAAGAC 2 cut(s) 112, 668
BstX2I RGATCY 1 cut(s) 1485
BstXI CCANNNNNNTGG 2 cut(s) 1667, 1785
BstYI RGATCY 1 cut(s) 1485
BstZ17I GTATAC 1 cut(s) 1615
Bsu36I CCTNAGG 2 cut(s) 873, 1005
BsuI GTATCC 1 cut(s) 1707
BsuRI GGCC 6 cut(s) 159, 394, 462, 632, 646, 973
BtgI CCRYGG 2 cut(s) 373, 1740
BtsCI GGATG 5 cut(s) 550, 841, 1656, 1750, 1794
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 2 cut(s) 703, 919
CaiI CAGNNNCTG 1 cut(s) 1309
CciI TCATGA 1 cut(s) 1563
CfoI GCGC 3 cut(s) 358, 1054, 1248
Cfr13I GGNCC 3 cut(s) 158, 460, 1364
CseI GACGC 1 cut(s) 717
Csp6I GTAC 2 cut(s) 998, 1763
CviAII CATG 5 cut(s) 1253, 1427, 1495, 1564, 1661
CviQI GTAC 2 cut(s) 998, 1763
DdeI CTNAG 5 cut(s) 873, 1005, 1064, 1101, 1731
DpnI GATC 3 cut(s) 1158, 1487, 1689
DpnII GATC 3 cut(s) 1156, 1485, 1687
EaeI YGGCCR 3 cut(s) 630, 644, 971
Eam1104I CTCTTC 5 cut(s) 61, 171, 650, 750, 1469
EarI CTCTTC 5 cut(s) 61, 171, 650, 750, 1469
Eco130I CCWWGG 2 cut(s) 395, 1677
Eco47I GGWCC 1 cut(s) 1364
Eco57I CTGAAG 1 cut(s) 1159
Eco81I CCTNAGG 2 cut(s) 873, 1005
Eco88I CYCGRG 4 cut(s) 359, 744, 1316, 1469
EcoRI GAATTC 2 cut(s) 1279, 1694
EcoRII CCWGG 4 cut(s) 421, 587, 1110, 1777
EcoT14I CCWWGG 2 cut(s) 395, 1677
EcoT22I ATGCAT 1 cut(s) 1603
ErhI CCWWGG 2 cut(s) 395, 1677
Esp3I CGTCTC 2 cut(s) 168, 374
FaeI CATG 5 cut(s) 1256, 1430, 1498, 1567, 1664
FalI AAGNNNNNCTT 2 cut(s) 267, 299
FaqI GGGAC 2 cut(s) 320, 1306
FatI CATG 5 cut(s) 1252, 1426, 1494, 1563, 1660
FblI GTMKAC 1 cut(s) 1614
Fnu4HI GCNGC 5 cut(s) 1187, 1310, 1380, 1383, 1584
FokI GGATG 5 cut(s) 557, 848, 1643, 1757, 1801
Fsp4HI GCNGC 5 cut(s) 1187, 1310, 1380, 1383, 1584
FspBI CTAG 9 cut(s) 191, 306, 396, 414, 935, 1190, 1226, 1266, 1595
FspI TGCGCA 1 cut(s) 1053
GlaI GCGC 3 cut(s) 357, 1053, 1247
GluI GCNGC 5 cut(s) 1187, 1310, 1380, 1383, 1584
GsuI CTGGAG 4 cut(s) 109, 444, 1377, 1761
HaeIII GGCC 6 cut(s) 159, 394, 462, 632, 646, 973
HapII CCGG 3 cut(s) 338, 517, 849
HgaI GACGC 1 cut(s) 717
HhaI GCGC 3 cut(s) 358, 1054, 1248
Hin1II CATG 5 cut(s) 1256, 1430, 1498, 1567, 1664
Hin6I GCGC 3 cut(s) 356, 1052, 1246
HinP1I GCGC 3 cut(s) 356, 1052, 1246
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 11, 919
HpaI GTTAAC 1 cut(s) 514
HpaII CCGG 3 cut(s) 338, 517, 849
HphI GGTGA 4 cut(s) 460, 863, 1231, 1543
Hpy166II GTNNAC 2 cut(s) 514, 1615
Hpy188I TCNGA 8 cut(s) 26, 171, 405, 623, 1059, 1178, 1687, 1732
Hpy188III TCNNGA 8 cut(s) 221, 361, 863, 1471, 1564, 1595, 1691, 1833
Hpy8I GTNNAC 2 cut(s) 514, 1615
HpyAV CCTTC 7 cut(s) 337, 533, 679, 776, 1032, 1631, 1837
HpyCH4III ACNGT 1 cut(s) 1141
HpyCH4IV ACGT 2 cut(s) 1335, 1399
HpyCH4V TGCA 8 cut(s) 571, 896, 917, 945, 1186, 1382, 1430, 1601
HpyF10VI GCNNNNNNNGC 3 cut(s) 908, 1051, 1379
HpyF3I CTNAG 5 cut(s) 873, 1005, 1064, 1101, 1731
HpySE526I ACGT 2 cut(s) 1335, 1399
Hsp92II CATG 5 cut(s) 1256, 1430, 1498, 1567, 1664
HspAI GCGC 3 cut(s) 356, 1052, 1246
KspAI GTTAAC 1 cut(s) 514
Kzo9I GATC 3 cut(s) 1156, 1485, 1687
LmnI GCTCC 1 cut(s) 1780
Lsp1109I GCAGC 5 cut(s) 1198, 1296, 1366, 1394, 1570
LweI GCATC 8 cut(s) 220, 904, 1063, 1084, 1570, 1588, 1743, 1779
MaeI CTAG 9 cut(s) 191, 306, 396, 414, 935, 1190, 1226, 1266, 1595
MaeII ACGT 2 cut(s) 1335, 1399
MaeIII GTNAC 2 cut(s) 1219, 1549
MalI GATC 3 cut(s) 1158, 1487, 1689
MbiI CCGCTC 1 cut(s) 1711
MboI GATC 3 cut(s) 1156, 1485, 1687
MfeI CAATTG 2 cut(s) 213, 267
MflI RGATCY 1 cut(s) 1485
MlsI TGGCCA 2 cut(s) 632, 973
MluNI TGGCCA 2 cut(s) 632, 973
MlyI GAGTC 2 cut(s) 342, 1216
Mox20I TGGCCA 2 cut(s) 632, 973
Mph1103I ATGCAT 1 cut(s) 1603
MroXI GAANNNNTTC 1 cut(s) 1210
MscI TGGCCA 2 cut(s) 632, 973
MseI TTAA 8 cut(s) 81, 387, 513, 597, 923, 1491, 1844, 1858
MslI CAYNNNNRTG 3 cut(s) 1632, 1661, 1665
Msp20I TGGCCA 2 cut(s) 632, 973
MspCI CTTAAG 2 cut(s) 922, 1843
MspI CCGG 3 cut(s) 338, 517, 849
MspR9I CCNGG 5 cut(s) 338, 423, 589, 1112, 1779
MunI CAATTG 2 cut(s) 213, 267
MvaI CCWGG 4 cut(s) 423, 589, 1112, 1779
MvnI CGCG 2 cut(s) 356, 1608
MwoI GCNNNNNNNGC 3 cut(s) 908, 1051, 1379
NciI CCSGG 1 cut(s) 338
NdeII GATC 3 cut(s) 1156, 1485, 1687
NlaIII CATG 5 cut(s) 1256, 1430, 1498, 1567, 1664
NlaIV GGNNCC 2 cut(s) 420, 1776
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 2 cut(s) 1219, 1549
NsbI TGCGCA 1 cut(s) 1053
NsiI ATGCAT 1 cut(s) 1603
NspV TTCGAA 1 cut(s) 1283
PaeR7I CTCGAG 3 cut(s) 359, 744, 1469
PagI TCATGA 1 cut(s) 1563
PcsI WCGNNNNNNNCGW 1 cut(s) 1704
PdmI GAANNNNTTC 1 cut(s) 1210
PflFI GACNNNGTC 1 cut(s) 1337
PflMI CCANNNNNTGG 1 cut(s) 1216
PfoI TCCNGGA 2 cut(s) 421, 1110
PkrI GCNGC 5 cut(s) 1188, 1311, 1381, 1384, 1585
PleI GAGTC 2 cut(s) 341, 1216
PpsI GAGTC 2 cut(s) 341, 1216
Psp6I CCWGG 4 cut(s) 421, 587, 1110, 1777
PspGI CCWGG 4 cut(s) 421, 587, 1110, 1777
PspN4I GGNNCC 2 cut(s) 420, 1776
PspPI GGNCC 3 cut(s) 158, 460, 1364
PspXI VCTCGAGB 1 cut(s) 744
PstI CTGCAG 1 cut(s) 1384
PstNI CAGNNNCTG 1 cut(s) 1309
PsuI RGATCY 1 cut(s) 1485
PsyI GACNNNGTC 1 cut(s) 1337
RsaI GTAC 2 cut(s) 999, 1764
RsaNI GTAC 2 cut(s) 998, 1763
RseI CAYNNNNRTG 3 cut(s) 1632, 1661, 1665
SaqAI TTAA 8 cut(s) 81, 387, 513, 597, 923, 1491, 1844, 1858
SatI GCNGC 5 cut(s) 1187, 1310, 1380, 1383, 1584
Sau3AI GATC 3 cut(s) 1156, 1485, 1687
Sau96I GGNCC 3 cut(s) 158, 460, 1364
SchI GAGTC 2 cut(s) 342, 1216
ScrFI CCNGG 5 cut(s) 338, 423, 589, 1112, 1779
SfaNI GCATC 8 cut(s) 220, 904, 1063, 1084, 1570, 1588, 1743, 1779
SfcI CTRYAG 1 cut(s) 1380
Sfr274I CTCGAG 3 cut(s) 359, 744, 1469
SfuI TTCGAA 1 cut(s) 1283
SinI GGWCC 1 cut(s) 1364
SlaI CTCGAG 3 cut(s) 359, 744, 1469
SmiMI CAYNNNNRTG 3 cut(s) 1632, 1661, 1665
SmlI CTYRAG 6 cut(s) 359, 744, 922, 1469, 1831, 1843
SmoI CTYRAG 6 cut(s) 359, 744, 922, 1469, 1831, 1843
SsiI CCGC 3 cut(s) 722, 1608, 1711
SspI AATATT 1 cut(s) 845
SspMI CTAG 9 cut(s) 191, 306, 396, 414, 935, 1190, 1226, 1266, 1595
StyD4I CCNGG 5 cut(s) 336, 421, 587, 1110, 1777
StyI CCWWGG 2 cut(s) 395, 1677
TaaI ACNGT 1 cut(s) 1141
TaiI ACGT 2 cut(s) 1338, 1402
TatI WGTACW 1 cut(s) 997
Tru1I TTAA 8 cut(s) 81, 387, 513, 597, 923, 1491, 1844, 1858
Tru9I TTAA 8 cut(s) 81, 387, 513, 597, 923, 1491, 1844, 1858
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 2 cut(s) 1219, 1549
TseI GCWGC 5 cut(s) 1186, 1309, 1379, 1382, 1583
Tsp45I GTSAC 2 cut(s) 1219, 1549
TspGWI ACGGA 4 cut(s) 141, 362, 1515, 1732
TspRI CASTG 1 cut(s) 570
Tth111I GACNNNGTC 1 cut(s) 1337
Van91I CCANNNNNTGG 1 cut(s) 1216
Vha464I CTTAAG 2 cut(s) 922, 1843
VpaK11BI GGWCC 1 cut(s) 1364
XapI RAATTY 6 cut(s) 299, 617, 1279, 1436, 1567, 1694
XbaI TCTAGA 1 cut(s) 1594
XhoI CTCGAG 3 cut(s) 359, 744, 1469
XmaJI CCTAGG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 1614
XmnI GAANNNNTTC 1 cut(s) 1210
XspI CTAG 9 cut(s) 191, 306, 396, 414, 935, 1190, 1226, 1266, 1595
Zsp2I ATGCAT 1 cut(s) 1603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.