Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 203 |
| AccB1I | GGYRCC | 1 cut(s) | 209 |
| AccBSI | CCGCTC | 2 cut(s) | 140, 844 |
| AccI | GTMKAC | 1 cut(s) | 1407 |
| AccII | CGCG | 2 cut(s) | 320, 595 |
| AccIII | TCCGGA | 1 cut(s) | 1693 |
| AclWI | GGATC | 4 cut(s) | 810, 823, 1654, 1685 |
| AcoI | YGGCCR | 1 cut(s) | 6 |
| AcsI | RAATTY | 5 cut(s) | 62, 156, 1282, 1570, 1643 |
| AcuI | CTGAAG | 4 cut(s) | 283, 693, 1011, 1029 |
| AfaI | GTAC | 5 cut(s) | 518, 563, 602, 971, 1767 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 205, 370 |
| AjnI | CCWGG | 3 cut(s) | 908, 1286, 1780 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 761, 793, 974, 1006 |
| Alw21I | GWGCWC | 1 cut(s) | 444 |
| Alw26I | GTCTC | 3 cut(s) | 118, 237, 465 |
| AlwI | GGATC | 4 cut(s) | 810, 823, 1654, 1685 |
| Ama87I | CYCGRG | 1 cut(s) | 356 |
| Aor13HI | TCCGGA | 1 cut(s) | 1693 |
| AoxI | GGCC | 6 cut(s) | 6, 373, 389, 635, 923, 1366 |
| ApeKI | GCWGC | 3 cut(s) | 829, 1003, 1586 |
| ApoI | RAATTY | 5 cut(s) | 62, 156, 1282, 1570, 1643 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 574, 606 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 584 |
| AspLEI | GCGC | 4 cut(s) | 204, 325, 1251, 1729 |
| AspS9I | GGNCC | 3 cut(s) | 191, 373, 1367 |
| AsuC2I | CCSGG | 2 cut(s) | 371, 420 |
| AsuHPI | GGTGA | 4 cut(s) | 227, 692, 758, 1120 |
| AsuNHI | GCTAGC | 1 cut(s) | 574 |
| AvaI | CYCGRG | 1 cut(s) | 356 |
| AvaII | GGWCC | 1 cut(s) | 191 |
| AxyI | CCTNAGG | 1 cut(s) | 876 |
| BaeGI | GKGCMC | 1 cut(s) | 212 |
| BalI | TGGCCA | 1 cut(s) | 8 |
| BamHI | GGATCC | 1 cut(s) | 815 |
| BanI | GGYRCC | 1 cut(s) | 209 |
| BanII | GRGCYC | 2 cut(s) | 444, 546 |
| BbsI | GAAGAC | 3 cut(s) | 486, 587, 1483 |
| Bbv12I | GWGCWC | 1 cut(s) | 444 |
| BbvI | GCAGC | 3 cut(s) | 816, 990, 1573 |
| BccI | CCATC | 2 cut(s) | 690, 1496 |
| BceAI | ACGGC | 1 cut(s) | 553 |
| BciT130I | CCWGG | 3 cut(s) | 910, 1288, 1782 |
| BclI | TGATCA | 1 cut(s) | 1461 |
| BcnI | CCSGG | 2 cut(s) | 371, 420 |
| BcoDI | GTCTC | 3 cut(s) | 118, 237, 465 |
| BfaI | CTAG | 3 cut(s) | 575, 1202, 1265 |
| BfmI | CTRYAG | 2 cut(s) | 1177, 1230 |
| BfoI | RGCGCY | 1 cut(s) | 326 |
| BisI | GCNGC | 8 cut(s) | 135, 138, 321, 830, 1004, 1386, 1587, 1740 |
| BlsI | GCNGC | 8 cut(s) | 136, 139, 322, 831, 1005, 1387, 1588, 1741 |
| BmcAI | AGTACT | 1 cut(s) | 971 |
| Bme1390I | CCNGG | 5 cut(s) | 371, 420, 910, 1288, 1782 |
| Bme18I | GGWCC | 1 cut(s) | 191 |
| BmeT110I | CYCGRG | 1 cut(s) | 356 |
| BmgT120I | GGNCC | 3 cut(s) | 191, 373, 1367 |
| BmiI | GGNNCC | 4 cut(s) | 193, 211, 374, 817 |
| BmrFI | CCNGG | 5 cut(s) | 371, 420, 910, 1288, 1782 |
| BmrI | ACTGGG | 1 cut(s) | 1368 |
| BmsI | GCATC | 5 cut(s) | 935, 1150, 1176, 1573, 1591 |
| BmtI | GCTAGC | 1 cut(s) | 578 |
| BmuI | ACTGGG | 1 cut(s) | 1368 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1145 |
| BpiI | GAAGAC | 3 cut(s) | 486, 587, 1483 |
| BpmI | CTGGAG | 1 cut(s) | 743 |
| BpuEI | CTTGAG | 2 cut(s) | 1542, 1671 |
| BpuMI | CCSGG | 2 cut(s) | 371, 420 |
| BsaAI | YACGTR | 1 cut(s) | 604 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1422 |
| BsaI | GGTCTC | 2 cut(s) | 237, 465 |
| BsaJI | CCNNGG | 3 cut(s) | 255, 370, 418 |
| BsaWI | WCCGGW | 2 cut(s) | 553, 1693 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 75, 105, 179, 209 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 205, 370 |
| Bse118I | RCCGGY | 1 cut(s) | 925 |
| Bse1I | ACTGG | 3 cut(s) | 392, 977, 1363 |
| Bse21I | CCTNAGG | 1 cut(s) | 876 |
| Bse3DI | GCAATG | 1 cut(s) | 1383 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1422 |
| BseAI | TCCGGA | 1 cut(s) | 1693 |
| BseBI | CCWGG | 3 cut(s) | 910, 1288, 1782 |
| BseDI | CCNNGG | 3 cut(s) | 255, 370, 418 |
| BseGI | GGATG | 7 cut(s) | 633, 667, 739, 790, 844, 1507, 1797 |
| BseJI | GATNNNNATC | 1 cut(s) | 1422 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 205, 370 |
| BseMI | GCAATG | 1 cut(s) | 1383 |
| BseMII | CTCAG | 4 cut(s) | 34, 867, 1058, 1746 |
| BseNI | ACTGG | 3 cut(s) | 392, 977, 1363 |
| BseRI | GAGGAG | 1 cut(s) | 881 |
| BseSI | GKGCMC | 1 cut(s) | 212 |
| BseXI | GCAGC | 3 cut(s) | 816, 990, 1573 |
| BseYI | CCCAGC | 1 cut(s) | 1205 |
| Bsh1236I | CGCG | 2 cut(s) | 320, 595 |
| BshFI | GGCC | 6 cut(s) | 8, 375, 391, 637, 925, 1368 |
| BshNI | GGYRCC | 1 cut(s) | 209 |
| BsiHKAI | GWGCWC | 1 cut(s) | 444 |
| BsiHKCI | CYCGRG | 1 cut(s) | 356 |
| BsiSI | CCGG | 5 cut(s) | 370, 420, 554, 926, 1694 |
| BslFI | GGGAC | 2 cut(s) | 317, 401 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 205, 370 |
| BsmAI | GTCTC | 3 cut(s) | 118, 237, 465 |
| BsmBI | CGTCTC | 1 cut(s) | 118 |
| BsmFI | GGGAC | 2 cut(s) | 317, 401 |
| BsmI | GAATGC | 1 cut(s) | 396 |
| BsnI | GGCC | 6 cut(s) | 8, 375, 391, 637, 925, 1368 |
| Bso31I | GGTCTC | 2 cut(s) | 237, 465 |
| BsoBI | CYCGRG | 1 cut(s) | 356 |
| Bsp1286I | GDGCHC | 3 cut(s) | 212, 444, 546 |
| Bsp13I | TCCGGA | 1 cut(s) | 1693 |
| Bsp1407I | TGTACA | 1 cut(s) | 561 |
| Bsp143I | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| BspANI | GGCC | 6 cut(s) | 8, 375, 391, 637, 925, 1368 |
| BspCNI | CTCAG | 4 cut(s) | 33, 868, 1059, 1745 |
| BspEI | TCCGGA | 1 cut(s) | 1693 |
| BspFNI | CGCG | 2 cut(s) | 320, 595 |
| BspLI | GGNNCC | 4 cut(s) | 193, 211, 374, 817 |
| BspOI | GCTAGC | 1 cut(s) | 578 |
| BspPI | GGATC | 4 cut(s) | 810, 823, 1654, 1685 |
| BspQI | GCTCTTC | 1 cut(s) | 698 |
| BspT107I | GGYRCC | 1 cut(s) | 209 |
| BspTNI | GGTCTC | 2 cut(s) | 237, 465 |
| BsrBI | CCGCTC | 2 cut(s) | 140, 844 |
| BsrDI | GCAATG | 1 cut(s) | 1383 |
| BsrFI | RCCGGY | 1 cut(s) | 925 |
| BsrGI | TGTACA | 1 cut(s) | 561 |
| BsrI | ACTGG | 3 cut(s) | 392, 977, 1363 |
| BssAI | RCCGGY | 1 cut(s) | 925 |
| BssECI | CCNNGG | 3 cut(s) | 255, 370, 418 |
| BssMI | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| BssNAI | GTATAC | 1 cut(s) | 1408 |
| Bst1107I | GTATAC | 1 cut(s) | 1408 |
| Bst2UI | CCWGG | 3 cut(s) | 910, 1288, 1782 |
| Bst4CI | ACNGT | 7 cut(s) | 347, 825, 958, 1042, 1144, 1231, 1273 |
| Bst6I | CTCTTC | 7 cut(s) | 42, 285, 463, 647, 686, 698, 735 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1382 |
| BstAUI | TGTACA | 1 cut(s) | 561 |
| BstBAI | YACGTR | 1 cut(s) | 604 |
| BstC8I | GCNNGC | 5 cut(s) | 444, 542, 576, 927, 1293 |
| BstDEI | CTNAG | 6 cut(s) | 20, 876, 1067, 1545, 1732, 1818 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 203 |
| BstF5I | GGATG | 7 cut(s) | 633, 667, 739, 790, 844, 1507, 1797 |
| BstFNI | CGCG | 2 cut(s) | 320, 595 |
| BstH2I | RGCGCY | 1 cut(s) | 326 |
| BstHHI | GCGC | 4 cut(s) | 204, 325, 1251, 1729 |
| BstKTI | GATC | 4 cut(s) | 818, 1464, 1662, 1693 |
| BstMAI | GTCTC | 3 cut(s) | 118, 237, 465 |
| BstMBI | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 134, 388, 408, 443, 448, 1382, 1529 |
| BstNI | CCWGG | 3 cut(s) | 910, 1288, 1782 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1145 |
| BstSCI | CCNGG | 5 cut(s) | 369, 418, 908, 1286, 1780 |
| BstSFI | CTRYAG | 2 cut(s) | 1177, 1230 |
| BstSLI | GKGCMC | 1 cut(s) | 212 |
| BstUI | CGCG | 2 cut(s) | 320, 595 |
| BstV1I | GCAGC | 3 cut(s) | 816, 990, 1573 |
| BstV2I | GAAGAC | 3 cut(s) | 486, 587, 1483 |
| BstX2I | RGATCY | 2 cut(s) | 815, 1690 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1670, 1788 |
| BstYI | RGATCY | 2 cut(s) | 815, 1690 |
| BstZ17I | GTATAC | 1 cut(s) | 1408 |
| Bsu36I | CCTNAGG | 1 cut(s) | 876 |
| BsuRI | GGCC | 6 cut(s) | 8, 375, 391, 637, 925, 1368 |
| BtgZI | GCGATG | 2 cut(s) | 301, 908 |
| BtsCI | GGATG | 7 cut(s) | 633, 667, 739, 790, 844, 1507, 1797 |
| BtsIMutI | CAGTG | 2 cut(s) | 1278, 1356 |
| Cac8I | GCNNGC | 5 cut(s) | 444, 542, 576, 927, 1293 |
| CfoI | GCGC | 4 cut(s) | 204, 325, 1251, 1729 |
| Cfr10I | RCCGGY | 1 cut(s) | 925 |
| Cfr13I | GGNCC | 3 cut(s) | 191, 373, 1367 |
| CseI | GACGC | 1 cut(s) | 1791 |
| Csp6I | GTAC | 5 cut(s) | 517, 562, 601, 970, 1766 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 870, 905 |
| CviAII | CATG | 3 cut(s) | 1256, 1498, 1664 |
| CviQI | GTAC | 5 cut(s) | 517, 562, 601, 970, 1766 |
| DdeI | CTNAG | 6 cut(s) | 20, 876, 1067, 1545, 1732, 1818 |
| DpnI | GATC | 4 cut(s) | 817, 1463, 1661, 1692 |
| DpnII | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| EaeI | YGGCCR | 1 cut(s) | 6 |
| Eam1104I | CTCTTC | 7 cut(s) | 42, 285, 463, 647, 686, 698, 735 |
| EarI | CTCTTC | 7 cut(s) | 42, 285, 463, 647, 686, 698, 735 |
| EciI | GGCGGA | 1 cut(s) | 115 |
| Ecl136II | GAGCTC | 1 cut(s) | 442 |
| Eco24I | GRGCYC | 2 cut(s) | 444, 546 |
| Eco31I | GGTCTC | 2 cut(s) | 237, 465 |
| Eco47I | GGWCC | 1 cut(s) | 191 |
| Eco53kI | GAGCTC | 1 cut(s) | 442 |
| Eco57I | CTGAAG | 4 cut(s) | 283, 693, 1011, 1029 |
| Eco81I | CCTNAGG | 1 cut(s) | 876 |
| Eco88I | CYCGRG | 1 cut(s) | 356 |
| EcoICRI | GAGCTC | 1 cut(s) | 442 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 203 |
| EcoRI | GAATTC | 1 cut(s) | 1282 |
| EcoRII | CCWGG | 3 cut(s) | 908, 1286, 1780 |
| EcoT22I | ATGCAT | 1 cut(s) | 1606 |
| EcoT38I | GRGCYC | 2 cut(s) | 444, 546 |
| Esp3I | CGTCTC | 1 cut(s) | 118 |
| FaeI | CATG | 3 cut(s) | 1259, 1501, 1667 |
| FaqI | GGGAC | 2 cut(s) | 317, 401 |
| FatI | CATG | 3 cut(s) | 1255, 1497, 1663 |
| FauI | CCCGC | 1 cut(s) | 588 |
| FbaI | TGATCA | 1 cut(s) | 1461 |
| FblI | GTMKAC | 1 cut(s) | 1407 |
| Fnu4HI | GCNGC | 8 cut(s) | 135, 138, 321, 830, 1004, 1386, 1587, 1740 |
| FokI | GGATG | 7 cut(s) | 640, 674, 746, 797, 851, 1514, 1804 |
| FriOI | GRGCYC | 2 cut(s) | 444, 546 |
| Fsp4HI | GCNGC | 8 cut(s) | 135, 138, 321, 830, 1004, 1386, 1587, 1740 |
| FspBI | CTAG | 3 cut(s) | 575, 1202, 1265 |
| FspI | TGCGCA | 1 cut(s) | 203 |
| GlaI | GCGC | 4 cut(s) | 203, 324, 1250, 1728 |
| GluI | GCNGC | 8 cut(s) | 135, 138, 321, 830, 1004, 1386, 1587, 1740 |
| GsaI | CCCAGC | 1 cut(s) | 1209 |
| GsuI | CTGGAG | 1 cut(s) | 743 |
| HaeII | RGCGCY | 1 cut(s) | 326 |
| HaeIII | GGCC | 6 cut(s) | 8, 375, 391, 637, 925, 1368 |
| HapII | CCGG | 5 cut(s) | 370, 420, 554, 926, 1694 |
| HgaI | GACGC | 1 cut(s) | 1791 |
| HhaI | GCGC | 4 cut(s) | 204, 325, 1251, 1729 |
| Hin1II | CATG | 3 cut(s) | 1259, 1501, 1667 |
| Hin6I | GCGC | 4 cut(s) | 202, 323, 1249, 1727 |
| HinP1I | GCGC | 4 cut(s) | 202, 323, 1249, 1727 |
| HincII | GTYRAC | 1 cut(s) | 1261 |
| HindII | GTYRAC | 1 cut(s) | 1261 |
| HindIII | AAGCTT | 1 cut(s) | 1094 |
| HpaII | CCGG | 5 cut(s) | 370, 420, 554, 926, 1694 |
| HphI | GGTGA | 4 cut(s) | 227, 692, 758, 1120 |
| Hpy166II | GTNNAC | 3 cut(s) | 1261, 1318, 1408 |
| Hpy188I | TCNGA | 5 cut(s) | 23, 614, 1140, 1713, 1735 |
| Hpy188III | TCNNGA | 7 cut(s) | 247, 358, 1115, 1445, 1598, 1688, 1694 |
| Hpy8I | GTNNAC | 3 cut(s) | 1261, 1318, 1408 |
| Hpy99I | CGWCG | 5 cut(s) | 272, 275, 278, 428, 539 |
| HpyAV | CCTTC | 8 cut(s) | 253, 668, 677, 743, 749, 1004, 1035, 1500 |
| HpyCH4III | ACNGT | 7 cut(s) | 347, 825, 958, 1042, 1144, 1231, 1273 |
| HpyCH4IV | ACGT | 2 cut(s) | 584, 603 |
| HpyCH4V | TGCA | 8 cut(s) | 338, 948, 1055, 1295, 1532, 1604, 1778, 1835 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 134, 388, 408, 443, 448, 1382, 1529 |
| HpyF3I | CTNAG | 6 cut(s) | 20, 876, 1067, 1545, 1732, 1818 |
| HpySE526I | ACGT | 2 cut(s) | 584, 603 |
| Hsp92II | CATG | 3 cut(s) | 1259, 1501, 1667 |
| HspAI | GCGC | 4 cut(s) | 202, 323, 1249, 1727 |
| Kpn2I | TCCGGA | 1 cut(s) | 1693 |
| KroI | GCCGGC | 1 cut(s) | 925 |
| KroNI | GCCGGC | 1 cut(s) | 927 |
| Ksp22I | TGATCA | 1 cut(s) | 1461 |
| Kzo9I | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| LguI | GCTCTTC | 1 cut(s) | 698 |
| Lsp1109I | GCAGC | 3 cut(s) | 816, 990, 1573 |
| LweI | GCATC | 5 cut(s) | 935, 1150, 1176, 1573, 1591 |
| MaeI | CTAG | 3 cut(s) | 575, 1202, 1265 |
| MaeII | ACGT | 2 cut(s) | 584, 603 |
| MaeIII | GTNAC | 1 cut(s) | 1222 |
| MalI | GATC | 4 cut(s) | 817, 1463, 1661, 1692 |
| MbiI | CCGCTC | 2 cut(s) | 140, 844 |
| MboI | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| MfeI | CAATTG | 1 cut(s) | 1434 |
| MflI | RGATCY | 2 cut(s) | 815, 1690 |
| MhlI | GDGCHC | 3 cut(s) | 212, 444, 546 |
| MlsI | TGGCCA | 1 cut(s) | 8 |
| MluCI | AATT | 9 cut(s) | 62, 156, 172, 1155, 1282, 1434, 1570, 1643, 1722 |
| MluNI | TGGCCA | 1 cut(s) | 8 |
| MlyI | GAGTC | 4 cut(s) | 339, 360, 423, 1185 |
| Mox20I | TGGCCA | 1 cut(s) | 8 |
| Mph1103I | ATGCAT | 1 cut(s) | 1606 |
| MroI | TCCGGA | 1 cut(s) | 1693 |
| MroNI | GCCGGC | 1 cut(s) | 925 |
| MroXI | GAANNNNTTC | 1 cut(s) | 584 |
| MscI | TGGCCA | 1 cut(s) | 8 |
| MseI | TTAA | 2 cut(s) | 221, 1485 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1668 |
| Msp20I | TGGCCA | 1 cut(s) | 8 |
| MspI | CCGG | 5 cut(s) | 370, 420, 554, 926, 1694 |
| MspR9I | CCNGG | 5 cut(s) | 371, 420, 910, 1288, 1782 |
| MunI | CAATTG | 1 cut(s) | 1434 |
| Mva1269I | GAATGC | 1 cut(s) | 396 |
| MvaI | CCWGG | 3 cut(s) | 910, 1288, 1782 |
| MvnI | CGCG | 2 cut(s) | 320, 595 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 134, 388, 408, 443, 448, 1382, 1529 |
| NaeI | GCCGGC | 1 cut(s) | 927 |
| NciI | CCSGG | 2 cut(s) | 371, 420 |
| NdeII | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| NgoMIV | GCCGGC | 1 cut(s) | 925 |
| NheI | GCTAGC | 1 cut(s) | 574 |
| NlaIII | CATG | 3 cut(s) | 1259, 1501, 1667 |
| NlaIV | GGNNCC | 4 cut(s) | 193, 211, 374, 817 |
| NmeAIII | GCCGAG | 2 cut(s) | 463, 472 |
| NmuCI | GTSAC | 1 cut(s) | 1222 |
| NsbI | TGCGCA | 1 cut(s) | 203 |
| NsiI | ATGCAT | 1 cut(s) | 1606 |
| PaeR7I | CTCGAG | 1 cut(s) | 356 |
| PciSI | GCTCTTC | 1 cut(s) | 698 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 267, 273 |
| PctI | GAATGC | 1 cut(s) | 396 |
| PdiI | GCCGGC | 1 cut(s) | 927 |
| PdmI | GAANNNNTTC | 1 cut(s) | 584 |
| PfeI | GAWTC | 8 cut(s) | 102, 505, 590, 1111, 1135, 1210, 1423, 1674 |
| PkrI | GCNGC | 8 cut(s) | 136, 139, 322, 831, 1005, 1387, 1588, 1741 |
| PleI | GAGTC | 4 cut(s) | 338, 360, 422, 1185 |
| PpsI | GAGTC | 4 cut(s) | 338, 360, 422, 1185 |
| Ppu21I | YACGTR | 1 cut(s) | 604 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1145 |
| Psp124BI | GAGCTC | 1 cut(s) | 444 |
| Psp6I | CCWGG | 3 cut(s) | 908, 1286, 1780 |
| PspFI | CCCAGC | 1 cut(s) | 1205 |
| PspGI | CCWGG | 3 cut(s) | 908, 1286, 1780 |
| PspN4I | GGNNCC | 4 cut(s) | 193, 211, 374, 817 |
| PspPI | GGNCC | 3 cut(s) | 191, 373, 1367 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 593, 625 |
| PsuI | RGATCY | 2 cut(s) | 815, 1690 |
| RsaI | GTAC | 5 cut(s) | 518, 563, 602, 971, 1767 |
| RsaNI | GTAC | 5 cut(s) | 517, 562, 601, 970, 1766 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1668 |
| SacI | GAGCTC | 1 cut(s) | 444 |
| SapI | GCTCTTC | 1 cut(s) | 698 |
| SaqAI | TTAA | 2 cut(s) | 221, 1485 |
| SatI | GCNGC | 8 cut(s) | 135, 138, 321, 830, 1004, 1386, 1587, 1740 |
| Sau3AI | GATC | 4 cut(s) | 815, 1461, 1659, 1690 |
| Sau96I | GGNCC | 3 cut(s) | 191, 373, 1367 |
| ScaI | AGTACT | 1 cut(s) | 971 |
| SchI | GAGTC | 4 cut(s) | 339, 360, 423, 1185 |
| ScrFI | CCNGG | 5 cut(s) | 371, 420, 910, 1288, 1782 |
| SduI | GDGCHC | 3 cut(s) | 212, 444, 546 |
| SfaNI | GCATC | 5 cut(s) | 935, 1150, 1176, 1573, 1591 |
| SfcI | CTRYAG | 2 cut(s) | 1177, 1230 |
| Sfr274I | CTCGAG | 1 cut(s) | 356 |
| SinI | GGWCC | 1 cut(s) | 191 |
| SlaI | CTCGAG | 1 cut(s) | 356 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1668 |
| SmlI | CTYRAG | 3 cut(s) | 356, 1557, 1686 |
| SmoI | CTYRAG | 3 cut(s) | 356, 1557, 1686 |
| Sse9I | AATT | 9 cut(s) | 62, 156, 172, 1155, 1282, 1434, 1570, 1643, 1722 |
| SspI | AATATT | 1 cut(s) | 1617 |
| SspMI | CTAG | 3 cut(s) | 575, 1202, 1265 |
| SstI | GAGCTC | 1 cut(s) | 444 |
| StyD4I | CCNGG | 5 cut(s) | 369, 418, 908, 1286, 1780 |
| TaaI | ACNGT | 7 cut(s) | 347, 825, 958, 1042, 1144, 1231, 1273 |
| TaiI | ACGT | 2 cut(s) | 587, 606 |
| TaqI | TCGA | 8 cut(s) | 179, 312, 328, 357, 477, 588, 632, 1194 |
| TasI | AATT | 9 cut(s) | 62, 156, 172, 1155, 1282, 1434, 1570, 1643, 1722 |
| TatI | WGTACW | 2 cut(s) | 561, 969 |
| TauI | GCSGC | 5 cut(s) | 137, 140, 323, 1388, 1742 |
| TfiI | GAWTC | 8 cut(s) | 102, 505, 590, 1111, 1135, 1210, 1423, 1674 |
| Tru1I | TTAA | 2 cut(s) | 221, 1485 |
| Tru9I | TTAA | 2 cut(s) | 221, 1485 |
| TscAI | CASTG | 2 cut(s) | 1278, 1363 |
| TseFI | GTSAC | 1 cut(s) | 1222 |
| TseI | GCWGC | 3 cut(s) | 829, 1003, 1586 |
| Tsp45I | GTSAC | 1 cut(s) | 1222 |
| TspDTI | ATGAA | 8 cut(s) | 911, 995, 1089, 1157, 1244, 1504, 1583, 1697 |
| TspGWI | ACGGA | 3 cut(s) | 204, 1345, 1649 |
| TspRI | CASTG | 2 cut(s) | 1278, 1363 |
| VpaK11BI | GGWCC | 1 cut(s) | 191 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 203 |
| XapI | RAATTY | 5 cut(s) | 62, 156, 1282, 1570, 1643 |
| XhoI | CTCGAG | 1 cut(s) | 356 |
| XmiI | GTMKAC | 1 cut(s) | 1407 |
| XmnI | GAANNNNTTC | 1 cut(s) | 584 |
| XspI | CTAG | 3 cut(s) | 575, 1202, 1265 |
| ZrmI | AGTACT | 1 cut(s) | 971 |
| Zsp2I | ATGCAT | 1 cut(s) | 1606 |