Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 363 |
| Acc36I | ACCTGC | 1 cut(s) | 363 |
| AccB1I | GGYRCC | 1 cut(s) | 188 |
| AccII | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| AccIII | TCCGGA | 1 cut(s) | 1672 |
| AclWI | GGATC | 3 cut(s) | 1602, 1633, 1664 |
| AcoI | YGGCCR | 3 cut(s) | 365, 385, 1024 |
| AcsI | RAATTY | 6 cut(s) | 62, 592, 1261, 1549, 1593, 1622 |
| AcuI | CTGAAG | 2 cut(s) | 259, 1686 |
| AcyI | GRCGYC | 1 cut(s) | 477 |
| AfaI | GTAC | 3 cut(s) | 503, 548, 1256 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 184, 346 |
| AflIII | ACRYGT | 1 cut(s) | 370 |
| AgsI | TTSAA | 9 cut(s) | 289, 748, 1016, 1148, 1175, 1297, 1402, 1418, 1815 |
| AjnI | CCWGG | 3 cut(s) | 603, 864, 1759 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 193, 225, 953, 985 |
| Alw21I | GWGCWC | 1 cut(s) | 429 |
| AlwI | GGATC | 3 cut(s) | 1602, 1633, 1664 |
| Ama87I | CYCGRG | 1 cut(s) | 332 |
| Aor13HI | TCCGGA | 1 cut(s) | 1672 |
| AoxI | GGCC | 7 cut(s) | 349, 365, 385, 620, 902, 1008, 1024 |
| ApeKI | GCWGC | 3 cut(s) | 1168, 1364, 1565 |
| ApoI | RAATTY | 6 cut(s) | 62, 592, 1261, 1549, 1593, 1622 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 569 |
| AspLEI | GCGC | 1 cut(s) | 582 |
| AspS9I | GGNCC | 4 cut(s) | 170, 349, 902, 1346 |
| AsuC2I | CCSGG | 2 cut(s) | 347, 396 |
| AsuHPI | GGTGA | 5 cut(s) | 655, 677, 704, 1099, 1555 |
| AvaI | CYCGRG | 1 cut(s) | 332 |
| AvaII | GGWCC | 2 cut(s) | 170, 1346 |
| AxyI | CCTNAGG | 1 cut(s) | 855 |
| BaeGI | GKGCMC | 1 cut(s) | 191 |
| BalI | TGGCCA | 1 cut(s) | 367 |
| BanI | GGYRCC | 1 cut(s) | 188 |
| BanII | GRGCYC | 1 cut(s) | 429 |
| BbsI | GAAGAC | 2 cut(s) | 417, 572 |
| Bbv12I | GWGCWC | 1 cut(s) | 429 |
| BbvI | GCAGC | 3 cut(s) | 1180, 1376, 1552 |
| BccI | CCATC | 4 cut(s) | 113, 780, 1475, 1695 |
| BceAI | ACGGC | 4 cut(s) | 490, 532, 538, 676 |
| BciT130I | CCWGG | 3 cut(s) | 605, 866, 1761 |
| BcnI | CCSGG | 2 cut(s) | 347, 396 |
| BfaI | CTAG | 2 cut(s) | 1244, 1577 |
| BfmI | CTRYAG | 3 cut(s) | 1156, 1362, 1566 |
| BfuAI | ACCTGC | 1 cut(s) | 363 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 348 |
| BisI | GCNGC | 7 cut(s) | 297, 340, 496, 530, 1169, 1365, 1566 |
| BlsI | GCNGC | 7 cut(s) | 298, 341, 497, 531, 1170, 1366, 1567 |
| Bme1390I | CCNGG | 5 cut(s) | 347, 396, 605, 866, 1761 |
| Bme18I | GGWCC | 2 cut(s) | 170, 1346 |
| BmeT110I | CYCGRG | 1 cut(s) | 332 |
| BmgT120I | GGNCC | 4 cut(s) | 170, 349, 902, 1346 |
| BmiI | GGNNCC | 6 cut(s) | 172, 190, 350, 602, 683, 903 |
| BmrFI | CCNGG | 5 cut(s) | 347, 396, 605, 866, 1761 |
| BmsI | GCATC | 4 cut(s) | 914, 1129, 1570, 1790 |
| BpiI | GAAGAC | 2 cut(s) | 417, 572 |
| BpmI | CTGGAG | 2 cut(s) | 728, 1359 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1083 |
| BpuMI | CCSGG | 2 cut(s) | 347, 396 |
| Bsa29I | ATCGAT | 1 cut(s) | 1137 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1401, 1686 |
| BsaHI | GRCGYC | 1 cut(s) | 477 |
| BsaJI | CCNNGG | 5 cut(s) | 346, 394, 1611, 1659, 1760 |
| BsaWI | WCCGGW | 2 cut(s) | 684, 1672 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 72, 102, 162, 192 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 184, 346 |
| Bse1I | ACTGG | 4 cut(s) | 368, 956, 1262, 1820 |
| Bse21I | CCTNAGG | 1 cut(s) | 855 |
| Bse3DI | GCAATG | 1 cut(s) | 1318 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1401, 1686 |
| BseAI | TCCGGA | 1 cut(s) | 1672 |
| BseBI | CCWGG | 3 cut(s) | 605, 866, 1761 |
| BseCI | ATCGAT | 1 cut(s) | 1137 |
| BseDI | CCNNGG | 5 cut(s) | 346, 394, 1611, 1659, 1760 |
| BseGI | GGATG | 7 cut(s) | 724, 769, 823, 1486, 1621, 1776, 1831 |
| BseJI | GATNNNNATC | 2 cut(s) | 1401, 1686 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 184, 346 |
| BseMI | GCAATG | 1 cut(s) | 1318 |
| BseMII | CTCAG | 4 cut(s) | 37, 524, 869, 1037 |
| BseNI | ACTGG | 4 cut(s) | 368, 956, 1262, 1820 |
| BseRI | GAGGAG | 5 cut(s) | 11, 260, 419, 652, 860 |
| BseSI | GKGCMC | 1 cut(s) | 191 |
| BseX3I | CGGCCG | 1 cut(s) | 385 |
| BseXI | GCAGC | 3 cut(s) | 1180, 1376, 1552 |
| BseYI | CCCAGC | 2 cut(s) | 757, 1184 |
| Bsh1236I | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| Bsh1285I | CGRYCG | 1 cut(s) | 388 |
| BshFI | GGCC | 7 cut(s) | 351, 367, 387, 622, 904, 1010, 1026 |
| BshNI | GGYRCC | 1 cut(s) | 188 |
| BshVI | ATCGAT | 1 cut(s) | 1137 |
| BsiEI | CGRYCG | 1 cut(s) | 388 |
| BsiHKAI | GWGCWC | 1 cut(s) | 429 |
| BsiHKCI | CYCGRG | 1 cut(s) | 332 |
| BsiSI | CCGG | 4 cut(s) | 346, 396, 685, 1673 |
| BslFI | GGGAC | 2 cut(s) | 293, 377 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 184, 346 |
| BsmFI | GGGAC | 2 cut(s) | 293, 377 |
| BsnI | GGCC | 7 cut(s) | 351, 367, 387, 622, 904, 1010, 1026 |
| BsoBI | CYCGRG | 1 cut(s) | 332 |
| Bsp1286I | GDGCHC | 2 cut(s) | 191, 429 |
| Bsp13I | TCCGGA | 1 cut(s) | 1672 |
| Bsp1407I | TGTACA | 1 cut(s) | 546 |
| Bsp143I | GATC | 3 cut(s) | 1607, 1638, 1669 |
| BspANI | GGCC | 7 cut(s) | 351, 367, 387, 622, 904, 1010, 1026 |
| BspCNI | CTCAG | 4 cut(s) | 36, 525, 868, 1038 |
| BspDI | ATCGAT | 1 cut(s) | 1137 |
| BspEI | TCCGGA | 1 cut(s) | 1672 |
| BspFNI | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| BspLI | GGNNCC | 6 cut(s) | 172, 190, 350, 602, 683, 903 |
| BspMAI | CTGCAG | 2 cut(s) | 1366, 1570 |
| BspMI | ACCTGC | 1 cut(s) | 363 |
| BspPI | GGATC | 3 cut(s) | 1602, 1633, 1664 |
| BspQI | GCTCTTC | 1 cut(s) | 20 |
| BspT107I | GGYRCC | 1 cut(s) | 188 |
| BsrDI | GCAATG | 1 cut(s) | 1318 |
| BsrGI | TGTACA | 1 cut(s) | 546 |
| BsrI | ACTGG | 4 cut(s) | 368, 956, 1262, 1820 |
| BssECI | CCNNGG | 5 cut(s) | 346, 394, 1611, 1659, 1760 |
| BssMI | GATC | 3 cut(s) | 1607, 1638, 1669 |
| BssNI | GRCGYC | 1 cut(s) | 477 |
| BssT1I | CCWWGG | 2 cut(s) | 1611, 1659 |
| Bst2UI | CCWGG | 3 cut(s) | 605, 866, 1761 |
| Bst4CI | ACNGT | 6 cut(s) | 323, 825, 1021, 1123, 1252, 1710 |
| Bst6I | CTCTTC | 8 cut(s) | 20, 50, 74, 453, 629, 671, 732, 776 |
| BstACI | GRCGYC | 1 cut(s) | 477 |
| BstAUI | TGTACA | 1 cut(s) | 546 |
| BstC8I | GCNNGC | 4 cut(s) | 527, 561, 906, 1631 |
| BstDEI | CTNAG | 5 cut(s) | 23, 533, 855, 1046, 1083 |
| BstEII | GGTNACC | 1 cut(s) | 4 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 182 |
| BstF5I | GGATG | 7 cut(s) | 724, 769, 823, 1486, 1621, 1776, 1831 |
| BstFNI | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| BstHHI | GCGC | 1 cut(s) | 582 |
| BstKTI | GATC | 3 cut(s) | 1610, 1641, 1672 |
| BstMBI | GATC | 3 cut(s) | 1607, 1638, 1669 |
| BstMCI | CGRYCG | 1 cut(s) | 388 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 348, 364, 384, 808, 866, 1308, 1361 |
| BstNI | CCWGG | 3 cut(s) | 605, 866, 1761 |
| BstPI | GGTNACC | 1 cut(s) | 4 |
| BstSCI | CCNGG | 5 cut(s) | 345, 394, 603, 864, 1759 |
| BstSFI | CTRYAG | 3 cut(s) | 1156, 1362, 1566 |
| BstSLI | GKGCMC | 1 cut(s) | 191 |
| BstUI | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| BstV1I | GCAGC | 3 cut(s) | 1180, 1376, 1552 |
| BstV2I | GAAGAC | 2 cut(s) | 417, 572 |
| BstX2I | RGATCY | 2 cut(s) | 1607, 1669 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1649, 1767 |
| BstYI | RGATCY | 2 cut(s) | 1607, 1669 |
| BstZI | CGGCCG | 1 cut(s) | 385 |
| Bsu15I | ATCGAT | 1 cut(s) | 1137 |
| Bsu36I | CCTNAGG | 1 cut(s) | 855 |
| BsuRI | GGCC | 7 cut(s) | 351, 367, 387, 622, 904, 1010, 1026 |
| BsuTUI | ATCGAT | 1 cut(s) | 1137 |
| BtgZI | GCGATG | 1 cut(s) | 277 |
| BtsCI | GGATG | 7 cut(s) | 724, 769, 823, 1486, 1621, 1776, 1831 |
| BtsIMutI | CAGTG | 3 cut(s) | 1257, 1706, 1827 |
| BveI | ACCTGC | 1 cut(s) | 363 |
| Cac8I | GCNNGC | 4 cut(s) | 527, 561, 906, 1631 |
| CfoI | GCGC | 1 cut(s) | 582 |
| Cfr13I | GGNCC | 4 cut(s) | 170, 349, 902, 1346 |
| ClaI | ATCGAT | 1 cut(s) | 1137 |
| CseI | GACGC | 4 cut(s) | 361, 466, 808, 1571 |
| Csp6I | GTAC | 3 cut(s) | 502, 547, 1255 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 1803 |
| CviAII | CATG | 4 cut(s) | 898, 1409, 1504, 1643 |
| CviQI | GTAC | 3 cut(s) | 502, 547, 1255 |
| DdeI | CTNAG | 5 cut(s) | 23, 533, 855, 1046, 1083 |
| DpnI | GATC | 3 cut(s) | 1609, 1640, 1671 |
| DpnII | GATC | 3 cut(s) | 1607, 1638, 1669 |
| EaeI | YGGCCR | 3 cut(s) | 365, 385, 1024 |
| EagI | CGGCCG | 1 cut(s) | 385 |
| Eam1104I | CTCTTC | 8 cut(s) | 20, 50, 74, 453, 629, 671, 732, 776 |
| EarI | CTCTTC | 8 cut(s) | 20, 50, 74, 453, 629, 671, 732, 776 |
| Ecl136II | GAGCTC | 1 cut(s) | 427 |
| EclXI | CGGCCG | 1 cut(s) | 385 |
| Eco130I | CCWWGG | 2 cut(s) | 1611, 1659 |
| Eco24I | GRGCYC | 1 cut(s) | 429 |
| Eco47I | GGWCC | 2 cut(s) | 170, 1346 |
| Eco52I | CGGCCG | 1 cut(s) | 385 |
| Eco53kI | GAGCTC | 1 cut(s) | 427 |
| Eco57I | CTGAAG | 2 cut(s) | 259, 1686 |
| Eco81I | CCTNAGG | 1 cut(s) | 855 |
| Eco88I | CYCGRG | 1 cut(s) | 332 |
| Eco91I | GGTNACC | 1 cut(s) | 4 |
| EcoICRI | GAGCTC | 1 cut(s) | 427 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 182 |
| EcoO109I | RGGNCCY | 1 cut(s) | 902 |
| EcoO65I | GGTNACC | 1 cut(s) | 4 |
| EcoRI | GAATTC | 1 cut(s) | 1261 |
| EcoRII | CCWGG | 3 cut(s) | 603, 864, 1759 |
| EcoT14I | CCWWGG | 2 cut(s) | 1611, 1659 |
| EcoT22I | ATGCAT | 1 cut(s) | 1585 |
| EcoT38I | GRGCYC | 1 cut(s) | 429 |
| ErhI | CCWWGG | 2 cut(s) | 1611, 1659 |
| FaeI | CATG | 4 cut(s) | 901, 1412, 1507, 1646 |
| FaqI | GGGAC | 2 cut(s) | 293, 377 |
| FatI | CATG | 4 cut(s) | 897, 1408, 1503, 1642 |
| FauI | CCCGC | 3 cut(s) | 319, 575, 1316 |
| Fnu4HI | GCNGC | 7 cut(s) | 297, 340, 496, 530, 1169, 1365, 1566 |
| FokI | GGATG | 6 cut(s) | 731, 776, 830, 1493, 1628, 1783 |
| FriOI | GRGCYC | 1 cut(s) | 429 |
| Fsp4HI | GCNGC | 7 cut(s) | 297, 340, 496, 530, 1169, 1365, 1566 |
| FspBI | CTAG | 2 cut(s) | 1244, 1577 |
| GlaI | GCGC | 1 cut(s) | 581 |
| GluI | GCNGC | 7 cut(s) | 297, 340, 496, 530, 1169, 1365, 1566 |
| GsaI | CCCAGC | 2 cut(s) | 761, 1188 |
| GsuI | CTGGAG | 2 cut(s) | 728, 1359 |
| HaeIII | GGCC | 7 cut(s) | 351, 367, 387, 622, 904, 1010, 1026 |
| HapII | CCGG | 4 cut(s) | 346, 396, 685, 1673 |
| HgaI | GACGC | 4 cut(s) | 361, 466, 808, 1571 |
| HhaI | GCGC | 1 cut(s) | 582 |
| Hin1I | GRCGYC | 1 cut(s) | 477 |
| Hin1II | CATG | 4 cut(s) | 901, 1412, 1507, 1646 |
| Hin6I | GCGC | 1 cut(s) | 580 |
| HinP1I | GCGC | 1 cut(s) | 580 |
| HincII | GTYRAC | 1 cut(s) | 1240 |
| HindII | GTYRAC | 1 cut(s) | 1240 |
| HindIII | AAGCTT | 1 cut(s) | 271 |
| HpaII | CCGG | 4 cut(s) | 346, 396, 685, 1673 |
| HphI | GGTGA | 5 cut(s) | 655, 677, 704, 1099, 1555 |
| Hpy166II | GTNNAC | 3 cut(s) | 502, 1240, 1255 |
| Hpy188I | TCNGA | 7 cut(s) | 26, 305, 514, 600, 1119, 1692, 1714 |
| Hpy188III | TCNNGA | 7 cut(s) | 149, 334, 1094, 1266, 1338, 1577, 1673 |
| Hpy8I | GTNNAC | 3 cut(s) | 502, 1240, 1255 |
| Hpy99I | CGWCG | 6 cut(s) | 266, 404, 482, 518, 524, 802 |
| HpyAV | CCTTC | 8 cut(s) | 130, 662, 682, 742, 871, 983, 1014, 1479 |
| HpyCH4III | ACNGT | 6 cut(s) | 323, 825, 1021, 1123, 1252, 1710 |
| HpyCH4IV | ACGT | 1 cut(s) | 569 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 348, 364, 384, 808, 866, 1308, 1361 |
| HpyF3I | CTNAG | 5 cut(s) | 23, 533, 855, 1046, 1083 |
| HpySE526I | ACGT | 1 cut(s) | 569 |
| Hsp92I | GRCGYC | 1 cut(s) | 477 |
| Hsp92II | CATG | 4 cut(s) | 901, 1412, 1507, 1646 |
| HspAI | GCGC | 1 cut(s) | 580 |
| Kpn2I | TCCGGA | 1 cut(s) | 1672 |
| Kzo9I | GATC | 3 cut(s) | 1607, 1638, 1669 |
| LguI | GCTCTTC | 1 cut(s) | 20 |
| LmnI | GCTCC | 3 cut(s) | 93, 432, 987 |
| Lsp1109I | GCAGC | 3 cut(s) | 1180, 1376, 1552 |
| LweI | GCATC | 4 cut(s) | 914, 1129, 1570, 1790 |
| MaeI | CTAG | 2 cut(s) | 1244, 1577 |
| MaeII | ACGT | 1 cut(s) | 569 |
| MaeIII | GTNAC | 3 cut(s) | 4, 643, 1201 |
| MalI | GATC | 3 cut(s) | 1609, 1640, 1671 |
| MboI | GATC | 3 cut(s) | 1607, 1638, 1669 |
| MflI | RGATCY | 2 cut(s) | 1607, 1669 |
| MhlI | GDGCHC | 2 cut(s) | 191, 429 |
| MlsI | TGGCCA | 1 cut(s) | 367 |
| MluCI | AATT | 7 cut(s) | 62, 143, 592, 1261, 1549, 1593, 1622 |
| MluI | ACGCGT | 1 cut(s) | 370 |
| MluNI | TGGCCA | 1 cut(s) | 367 |
| MlyI | GAGTC | 4 cut(s) | 315, 399, 569, 928 |
| MmeI | TCCRAC | 1 cut(s) | 537 |
| Mox20I | TGGCCA | 1 cut(s) | 367 |
| Mph1103I | ATGCAT | 1 cut(s) | 1585 |
| MroI | TCCGGA | 1 cut(s) | 1672 |
| MroXI | GAANNNNTTC | 1 cut(s) | 569 |
| MscI | TGGCCA | 1 cut(s) | 367 |
| MseI | TTAA | 4 cut(s) | 489, 1464, 1473, 1598 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1647 |
| Msp20I | TGGCCA | 1 cut(s) | 367 |
| MspA1I | CMGCKG | 1 cut(s) | 532 |
| MspI | CCGG | 4 cut(s) | 346, 396, 685, 1673 |
| MspR9I | CCNGG | 5 cut(s) | 347, 396, 605, 866, 1761 |
| MvaI | CCWGG | 3 cut(s) | 605, 866, 1761 |
| MvnI | CGCG | 5 cut(s) | 372, 498, 580, 582, 802 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 348, 364, 384, 808, 866, 1308, 1361 |
| NciI | CCSGG | 2 cut(s) | 347, 396 |
| NdeII | GATC | 3 cut(s) | 1607, 1638, 1669 |
| NlaIII | CATG | 4 cut(s) | 901, 1412, 1507, 1646 |
| NlaIV | GGNNCC | 6 cut(s) | 172, 190, 350, 602, 683, 903 |
| NmeAIII | GCCGAG | 2 cut(s) | 413, 448 |
| NmuCI | GTSAC | 3 cut(s) | 4, 643, 1201 |
| NsiI | ATGCAT | 1 cut(s) | 1585 |
| PaeR7I | CTCGAG | 1 cut(s) | 332 |
| PaqCI | CACCTGC | 1 cut(s) | 363 |
| PciSI | GCTCTTC | 1 cut(s) | 20 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 240, 420, 510, 522 |
| PdmI | GAANNNNTTC | 1 cut(s) | 569 |
| PfeI | GAWTC | 7 cut(s) | 99, 1090, 1114, 1189, 1402, 1653, 1701 |
| PflFI | GACNNNGTC | 1 cut(s) | 404 |
| PkrI | GCNGC | 7 cut(s) | 298, 341, 497, 531, 1170, 1366, 1567 |
| PleI | GAGTC | 4 cut(s) | 314, 398, 569, 928 |
| PpsI | GAGTC | 4 cut(s) | 314, 398, 569, 928 |
| Psp124BI | GAGCTC | 1 cut(s) | 429 |
| Psp6I | CCWGG | 3 cut(s) | 603, 864, 1759 |
| PspEI | GGTNACC | 1 cut(s) | 4 |
| PspFI | CCCAGC | 2 cut(s) | 757, 1184 |
| PspGI | CCWGG | 3 cut(s) | 603, 864, 1759 |
| PspN4I | GGNNCC | 6 cut(s) | 172, 190, 350, 602, 683, 903 |
| PspPI | GGNCC | 4 cut(s) | 170, 349, 902, 1346 |
| PstI | CTGCAG | 2 cut(s) | 1366, 1570 |
| PsuI | RGATCY | 2 cut(s) | 1607, 1669 |
| PsyI | GACNNNGTC | 1 cut(s) | 404 |
| RsaI | GTAC | 3 cut(s) | 503, 548, 1256 |
| RsaNI | GTAC | 3 cut(s) | 502, 547, 1255 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1647 |
| SacI | GAGCTC | 1 cut(s) | 429 |
| SapI | GCTCTTC | 1 cut(s) | 20 |
| SaqAI | TTAA | 4 cut(s) | 489, 1464, 1473, 1598 |
| SatI | GCNGC | 7 cut(s) | 297, 340, 496, 530, 1169, 1365, 1566 |
| Sau3AI | GATC | 3 cut(s) | 1607, 1638, 1669 |
| Sau96I | GGNCC | 4 cut(s) | 170, 349, 902, 1346 |
| SchI | GAGTC | 4 cut(s) | 315, 399, 569, 928 |
| ScrFI | CCNGG | 5 cut(s) | 347, 396, 605, 866, 1761 |
| SduI | GDGCHC | 2 cut(s) | 191, 429 |
| SfaNI | GCATC | 4 cut(s) | 914, 1129, 1570, 1790 |
| SfcI | CTRYAG | 3 cut(s) | 1156, 1362, 1566 |
| Sfr274I | CTCGAG | 1 cut(s) | 332 |
| SinI | GGWCC | 2 cut(s) | 170, 1346 |
| SlaI | CTCGAG | 1 cut(s) | 332 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1647 |
| SmlI | CTYRAG | 1 cut(s) | 332 |
| SmoI | CTYRAG | 1 cut(s) | 332 |
| Sse9I | AATT | 7 cut(s) | 62, 143, 592, 1261, 1549, 1593, 1622 |
| SspMI | CTAG | 2 cut(s) | 1244, 1577 |
| SstI | GAGCTC | 1 cut(s) | 429 |
| StyD4I | CCNGG | 5 cut(s) | 345, 394, 603, 864, 1759 |
| StyI | CCWWGG | 2 cut(s) | 1611, 1659 |
| TaaI | ACNGT | 6 cut(s) | 323, 825, 1021, 1123, 1252, 1710 |
| TaiI | ACGT | 1 cut(s) | 572 |
| TaqI | TCGA | 9 cut(s) | 234, 261, 333, 408, 573, 617, 797, 1137, 1452 |
| TasI | AATT | 7 cut(s) | 62, 143, 592, 1261, 1549, 1593, 1622 |
| TatI | WGTACW | 2 cut(s) | 546, 1254 |
| TauI | GCSGC | 4 cut(s) | 299, 342, 498, 532 |
| TfiI | GAWTC | 7 cut(s) | 99, 1090, 1114, 1189, 1402, 1653, 1701 |
| Tru1I | TTAA | 4 cut(s) | 489, 1464, 1473, 1598 |
| Tru9I | TTAA | 4 cut(s) | 489, 1464, 1473, 1598 |
| TscAI | CASTG | 3 cut(s) | 1257, 1713, 1827 |
| TseFI | GTSAC | 3 cut(s) | 4, 643, 1201 |
| TseI | GCWGC | 3 cut(s) | 1168, 1364, 1565 |
| Tsp45I | GTSAC | 3 cut(s) | 4, 643, 1201 |
| TspDTI | ATGAA | 9 cut(s) | 37, 735, 834, 890, 974, 1068, 1483, 1562, 1632 |
| TspGWI | ACGGA | 1 cut(s) | 662 |
| TspRI | CASTG | 3 cut(s) | 1257, 1713, 1827 |
| Tth111I | GACNNNGTC | 1 cut(s) | 404 |
| VpaK11BI | GGWCC | 2 cut(s) | 170, 1346 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 182 |
| XapI | RAATTY | 6 cut(s) | 62, 592, 1261, 1549, 1593, 1622 |
| XbaI | TCTAGA | 1 cut(s) | 1576 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1537 |
| XhoI | CTCGAG | 1 cut(s) | 332 |
| XmnI | GAANNNNTTC | 1 cut(s) | 569 |
| XspI | CTAG | 2 cut(s) | 1244, 1577 |
| Zsp2I | ATGCAT | 1 cut(s) | 1585 |