Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 369 |
| Acc36I | ACCTGC | 1 cut(s) | 369 |
| AccB1I | GGYRCC | 1 cut(s) | 194 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1838 |
| AccI | GTMKAC | 1 cut(s) | 940 |
| AccII | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| AccIII | TCCGGA | 1 cut(s) | 1684 |
| AclWI | GGATC | 3 cut(s) | 1614, 1645, 1676 |
| AcoI | YGGCCR | 2 cut(s) | 391, 1036 |
| AcsI | RAATTY | 6 cut(s) | 62, 598, 1273, 1561, 1605, 1634 |
| AcuI | CTGAAG | 2 cut(s) | 265, 1698 |
| AcyI | GRCGYC | 1 cut(s) | 483 |
| AfaI | GTAC | 3 cut(s) | 509, 554, 1268 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 190, 352, 1838 |
| AgsI | TTSAA | 9 cut(s) | 295, 760, 1028, 1160, 1187, 1309, 1414, 1430, 1827 |
| AjnI | CCWGG | 3 cut(s) | 609, 876, 1771 |
| Alw21I | GWGCWC | 1 cut(s) | 435 |
| AlwI | GGATC | 3 cut(s) | 1614, 1645, 1676 |
| Ama87I | CYCGRG | 1 cut(s) | 338 |
| Aor13HI | TCCGGA | 1 cut(s) | 1684 |
| AoxI | GGCC | 6 cut(s) | 355, 371, 391, 626, 1020, 1036 |
| ApeKI | GCWGC | 3 cut(s) | 1180, 1376, 1577 |
| ApoI | RAATTY | 6 cut(s) | 62, 598, 1273, 1561, 1605, 1634 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 575 |
| AspLEI | GCGC | 2 cut(s) | 588, 1514 |
| AspS9I | GGNCC | 3 cut(s) | 176, 355, 1358 |
| AsuC2I | CCSGG | 2 cut(s) | 353, 402 |
| AsuHPI | GGTGA | 5 cut(s) | 217, 683, 710, 1111, 1567 |
| AvaI | CYCGRG | 1 cut(s) | 338 |
| AvaII | GGWCC | 2 cut(s) | 176, 1358 |
| AxyI | CCTNAGG | 1 cut(s) | 867 |
| BaeGI | GKGCMC | 1 cut(s) | 197 |
| BanI | GGYRCC | 1 cut(s) | 194 |
| BanII | GRGCYC | 1 cut(s) | 435 |
| BbsI | GAAGAC | 3 cut(s) | 423, 477, 578 |
| Bbv12I | GWGCWC | 1 cut(s) | 435 |
| BbvI | GCAGC | 3 cut(s) | 1192, 1388, 1564 |
| BccI | CCATC | 5 cut(s) | 113, 792, 1487, 1707, 1832 |
| BceAI | ACGGC | 4 cut(s) | 496, 538, 544, 682 |
| BciT130I | CCWGG | 3 cut(s) | 611, 878, 1773 |
| BcnI | CCSGG | 2 cut(s) | 353, 402 |
| BfaI | CTAG | 2 cut(s) | 1256, 1589 |
| BfmI | CTRYAG | 3 cut(s) | 1168, 1374, 1578 |
| BfoI | RGCGCY | 1 cut(s) | 1515 |
| BfuAI | ACCTGC | 1 cut(s) | 369 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 354 |
| BisI | GCNGC | 7 cut(s) | 303, 346, 502, 536, 1181, 1377, 1578 |
| BlsI | GCNGC | 7 cut(s) | 304, 347, 503, 537, 1182, 1378, 1579 |
| Bme1390I | CCNGG | 5 cut(s) | 353, 402, 611, 878, 1773 |
| Bme18I | GGWCC | 2 cut(s) | 176, 1358 |
| BmeT110I | CYCGRG | 1 cut(s) | 338 |
| BmgT120I | GGNCC | 3 cut(s) | 176, 355, 1358 |
| BmiI | GGNNCC | 5 cut(s) | 178, 196, 356, 608, 689 |
| BmrFI | CCNGG | 5 cut(s) | 353, 402, 611, 878, 1773 |
| BmsI | GCATC | 3 cut(s) | 1141, 1582, 1802 |
| BpiI | GAAGAC | 3 cut(s) | 423, 477, 578 |
| BpmI | CTGGAG | 2 cut(s) | 734, 1371 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1095 |
| BpuMI | CCSGG | 2 cut(s) | 353, 402 |
| Bsa29I | ATCGAT | 1 cut(s) | 1149 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1413 |
| BsaHI | GRCGYC | 1 cut(s) | 483 |
| BsaJI | CCNNGG | 4 cut(s) | 352, 400, 1623, 1772 |
| BsaWI | WCCGGW | 2 cut(s) | 690, 1684 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 72, 102 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 190, 352, 1838 |
| Bse1I | ACTGG | 4 cut(s) | 374, 968, 1274, 1832 |
| Bse21I | CCTNAGG | 1 cut(s) | 867 |
| Bse3DI | GCAATG | 1 cut(s) | 1330 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1413 |
| BseAI | TCCGGA | 1 cut(s) | 1684 |
| BseBI | CCWGG | 3 cut(s) | 611, 878, 1773 |
| BseCI | ATCGAT | 1 cut(s) | 1149 |
| BseDI | CCNNGG | 4 cut(s) | 352, 400, 1623, 1772 |
| BseGI | GGATG | 9 cut(s) | 105, 733, 781, 835, 1498, 1633, 1788, 1843, 1847 |
| BseJI | GATNNNNATC | 1 cut(s) | 1413 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 190, 352, 1838 |
| BseMI | GCAATG | 1 cut(s) | 1330 |
| BseMII | CTCAG | 4 cut(s) | 37, 530, 881, 1049 |
| BseNI | ACTGG | 4 cut(s) | 374, 968, 1274, 1832 |
| BseRI | GAGGAG | 5 cut(s) | 11, 266, 425, 762, 872 |
| BseSI | GKGCMC | 1 cut(s) | 197 |
| BseX3I | CGGCCG | 1 cut(s) | 391 |
| BseXI | GCAGC | 3 cut(s) | 1192, 1388, 1564 |
| BseYI | CCCAGC | 2 cut(s) | 769, 1196 |
| Bsh1236I | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| Bsh1285I | CGRYCG | 1 cut(s) | 394 |
| BshFI | GGCC | 6 cut(s) | 357, 373, 393, 628, 1022, 1038 |
| BshNI | GGYRCC | 1 cut(s) | 194 |
| BshVI | ATCGAT | 1 cut(s) | 1149 |
| BsiEI | CGRYCG | 1 cut(s) | 394 |
| BsiHKAI | GWGCWC | 1 cut(s) | 435 |
| BsiHKCI | CYCGRG | 1 cut(s) | 338 |
| BsiSI | CCGG | 4 cut(s) | 352, 402, 691, 1685 |
| BslFI | GGGAC | 3 cut(s) | 299, 383, 802 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 190, 352, 1838 |
| BsmFI | GGGAC | 3 cut(s) | 299, 383, 802 |
| BsnI | GGCC | 6 cut(s) | 357, 373, 393, 628, 1022, 1038 |
| BsoBI | CYCGRG | 1 cut(s) | 338 |
| Bsp1286I | GDGCHC | 2 cut(s) | 197, 435 |
| Bsp13I | TCCGGA | 1 cut(s) | 1684 |
| Bsp1407I | TGTACA | 1 cut(s) | 552 |
| Bsp143I | GATC | 3 cut(s) | 1619, 1650, 1681 |
| BspANI | GGCC | 6 cut(s) | 357, 373, 393, 628, 1022, 1038 |
| BspCNI | CTCAG | 4 cut(s) | 36, 531, 880, 1050 |
| BspDI | ATCGAT | 1 cut(s) | 1149 |
| BspEI | TCCGGA | 1 cut(s) | 1684 |
| BspFNI | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| BspLI | GGNNCC | 5 cut(s) | 178, 196, 356, 608, 689 |
| BspMAI | CTGCAG | 2 cut(s) | 1378, 1582 |
| BspMI | ACCTGC | 1 cut(s) | 369 |
| BspPI | GGATC | 3 cut(s) | 1614, 1645, 1676 |
| BspQI | GCTCTTC | 1 cut(s) | 20 |
| BspT107I | GGYRCC | 1 cut(s) | 194 |
| BsrDI | GCAATG | 1 cut(s) | 1330 |
| BsrGI | TGTACA | 1 cut(s) | 552 |
| BsrI | ACTGG | 4 cut(s) | 374, 968, 1274, 1832 |
| BssECI | CCNNGG | 4 cut(s) | 352, 400, 1623, 1772 |
| BssMI | GATC | 3 cut(s) | 1619, 1650, 1681 |
| BssNI | GRCGYC | 1 cut(s) | 483 |
| BssT1I | CCWWGG | 1 cut(s) | 1623 |
| Bst2UI | CCWGG | 3 cut(s) | 611, 878, 1773 |
| Bst4CI | ACNGT | 7 cut(s) | 329, 816, 837, 1033, 1135, 1264, 1722 |
| Bst6I | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 459, 677, 788 |
| BstACI | GRCGYC | 1 cut(s) | 483 |
| BstAUI | TGTACA | 1 cut(s) | 552 |
| BstC8I | GCNNGC | 3 cut(s) | 533, 567, 1643 |
| BstDEI | CTNAG | 5 cut(s) | 23, 539, 867, 1058, 1095 |
| BstEII | GGTNACC | 1 cut(s) | 4 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 188 |
| BstF5I | GGATG | 9 cut(s) | 105, 733, 781, 835, 1498, 1633, 1788, 1843, 1847 |
| BstFNI | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| BstH2I | RGCGCY | 1 cut(s) | 1515 |
| BstHHI | GCGC | 2 cut(s) | 588, 1514 |
| BstKTI | GATC | 3 cut(s) | 1622, 1653, 1684 |
| BstMBI | GATC | 3 cut(s) | 1619, 1650, 1681 |
| BstMCI | CGRYCG | 1 cut(s) | 394 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 354, 370, 390, 878, 1320, 1373, 1520 |
| BstNI | CCWGG | 3 cut(s) | 611, 878, 1773 |
| BstPI | GGTNACC | 1 cut(s) | 4 |
| BstSCI | CCNGG | 5 cut(s) | 351, 400, 609, 876, 1771 |
| BstSFI | CTRYAG | 3 cut(s) | 1168, 1374, 1578 |
| BstSLI | GKGCMC | 1 cut(s) | 197 |
| BstUI | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| BstV1I | GCAGC | 3 cut(s) | 1192, 1388, 1564 |
| BstV2I | GAAGAC | 3 cut(s) | 423, 477, 578 |
| BstX2I | RGATCY | 2 cut(s) | 1619, 1681 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1661, 1779 |
| BstYI | RGATCY | 2 cut(s) | 1619, 1681 |
| BstZI | CGGCCG | 1 cut(s) | 391 |
| Bsu15I | ATCGAT | 1 cut(s) | 1149 |
| Bsu36I | CCTNAGG | 1 cut(s) | 867 |
| BsuRI | GGCC | 6 cut(s) | 357, 373, 393, 628, 1022, 1038 |
| BsuTUI | ATCGAT | 1 cut(s) | 1149 |
| BtgZI | GCGATG | 2 cut(s) | 283, 420 |
| BtsCI | GGATG | 9 cut(s) | 105, 733, 781, 835, 1498, 1633, 1788, 1843, 1847 |
| BtsIMutI | CAGTG | 3 cut(s) | 1269, 1718, 1839 |
| BveI | ACCTGC | 1 cut(s) | 369 |
| Cac8I | GCNNGC | 3 cut(s) | 533, 567, 1643 |
| CfoI | GCGC | 2 cut(s) | 588, 1514 |
| Cfr13I | GGNCC | 3 cut(s) | 176, 355, 1358 |
| ClaI | ATCGAT | 1 cut(s) | 1149 |
| CseI | GACGC | 3 cut(s) | 367, 472, 1583 |
| Csp6I | GTAC | 3 cut(s) | 508, 553, 1267 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1815, 1850 |
| CviAII | CATG | 3 cut(s) | 910, 1516, 1655 |
| CviQI | GTAC | 3 cut(s) | 508, 553, 1267 |
| DdeI | CTNAG | 5 cut(s) | 23, 539, 867, 1058, 1095 |
| DpnI | GATC | 3 cut(s) | 1621, 1652, 1683 |
| DpnII | GATC | 3 cut(s) | 1619, 1650, 1681 |
| EaeI | YGGCCR | 2 cut(s) | 391, 1036 |
| EagI | CGGCCG | 1 cut(s) | 391 |
| Eam1104I | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 459, 677, 788 |
| EarI | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 459, 677, 788 |
| Ecl136II | GAGCTC | 1 cut(s) | 433 |
| EclXI | CGGCCG | 1 cut(s) | 391 |
| Eco130I | CCWWGG | 1 cut(s) | 1623 |
| Eco24I | GRGCYC | 1 cut(s) | 435 |
| Eco47I | GGWCC | 2 cut(s) | 176, 1358 |
| Eco52I | CGGCCG | 1 cut(s) | 391 |
| Eco53kI | GAGCTC | 1 cut(s) | 433 |
| Eco57I | CTGAAG | 2 cut(s) | 265, 1698 |
| Eco81I | CCTNAGG | 1 cut(s) | 867 |
| Eco88I | CYCGRG | 1 cut(s) | 338 |
| Eco91I | GGTNACC | 1 cut(s) | 4 |
| EcoICRI | GAGCTC | 1 cut(s) | 433 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 188 |
| EcoO65I | GGTNACC | 1 cut(s) | 4 |
| EcoRI | GAATTC | 1 cut(s) | 1273 |
| EcoRII | CCWGG | 3 cut(s) | 609, 876, 1771 |
| EcoT14I | CCWWGG | 1 cut(s) | 1623 |
| EcoT22I | ATGCAT | 1 cut(s) | 1597 |
| EcoT38I | GRGCYC | 1 cut(s) | 435 |
| ErhI | CCWWGG | 1 cut(s) | 1623 |
| FaeI | CATG | 3 cut(s) | 913, 1519, 1658 |
| FaqI | GGGAC | 3 cut(s) | 299, 383, 802 |
| FatI | CATG | 3 cut(s) | 909, 1515, 1654 |
| FauI | CCCGC | 3 cut(s) | 325, 581, 1328 |
| FblI | GTMKAC | 1 cut(s) | 940 |
| Fnu4HI | GCNGC | 7 cut(s) | 303, 346, 502, 536, 1181, 1377, 1578 |
| FokI | GGATG | 9 cut(s) | 92, 740, 788, 842, 1505, 1640, 1795, 1850, 1854 |
| FriOI | GRGCYC | 1 cut(s) | 435 |
| Fsp4HI | GCNGC | 7 cut(s) | 303, 346, 502, 536, 1181, 1377, 1578 |
| FspBI | CTAG | 2 cut(s) | 1256, 1589 |
| GlaI | GCGC | 2 cut(s) | 587, 1513 |
| GluI | GCNGC | 7 cut(s) | 303, 346, 502, 536, 1181, 1377, 1578 |
| GsaI | CCCAGC | 2 cut(s) | 773, 1200 |
| GsuI | CTGGAG | 2 cut(s) | 734, 1371 |
| HaeII | RGCGCY | 1 cut(s) | 1515 |
| HaeIII | GGCC | 6 cut(s) | 357, 373, 393, 628, 1022, 1038 |
| HapII | CCGG | 4 cut(s) | 352, 402, 691, 1685 |
| HgaI | GACGC | 3 cut(s) | 367, 472, 1583 |
| HhaI | GCGC | 2 cut(s) | 588, 1514 |
| Hin1I | GRCGYC | 1 cut(s) | 483 |
| Hin1II | CATG | 3 cut(s) | 913, 1519, 1658 |
| Hin6I | GCGC | 2 cut(s) | 586, 1512 |
| HinP1I | GCGC | 2 cut(s) | 586, 1512 |
| HincII | GTYRAC | 1 cut(s) | 1252 |
| HindII | GTYRAC | 1 cut(s) | 1252 |
| HindIII | AAGCTT | 1 cut(s) | 277 |
| HpaII | CCGG | 4 cut(s) | 352, 402, 691, 1685 |
| HphI | GGTGA | 5 cut(s) | 217, 683, 710, 1111, 1567 |
| Hpy166II | GTNNAC | 4 cut(s) | 508, 941, 1252, 1267 |
| Hpy188I | TCNGA | 7 cut(s) | 26, 311, 520, 606, 1131, 1704, 1726 |
| Hpy188III | TCNNGA | 7 cut(s) | 155, 340, 1106, 1278, 1350, 1589, 1685 |
| Hpy8I | GTNNAC | 4 cut(s) | 508, 941, 1252, 1267 |
| Hpy99I | CGWCG | 4 cut(s) | 272, 488, 524, 530 |
| HpyAV | CCTTC | 9 cut(s) | 130, 668, 688, 737, 754, 883, 995, 1026, 1491 |
| HpyCH4III | ACNGT | 7 cut(s) | 329, 816, 837, 1033, 1135, 1264, 1722 |
| HpyCH4IV | ACGT | 1 cut(s) | 575 |
| HpyCH4V | TGCA | 9 cut(s) | 1046, 1180, 1376, 1438, 1523, 1580, 1595, 1769, 1793 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 354, 370, 390, 878, 1320, 1373, 1520 |
| HpyF3I | CTNAG | 5 cut(s) | 23, 539, 867, 1058, 1095 |
| HpySE526I | ACGT | 1 cut(s) | 575 |
| Hsp92I | GRCGYC | 1 cut(s) | 483 |
| Hsp92II | CATG | 3 cut(s) | 913, 1519, 1658 |
| HspAI | GCGC | 2 cut(s) | 586, 1512 |
| Kpn2I | TCCGGA | 1 cut(s) | 1684 |
| Kzo9I | GATC | 3 cut(s) | 1619, 1650, 1681 |
| LguI | GCTCTTC | 1 cut(s) | 20 |
| LmnI | GCTCC | 3 cut(s) | 93, 438, 999 |
| Lsp1109I | GCAGC | 3 cut(s) | 1192, 1388, 1564 |
| LweI | GCATC | 3 cut(s) | 1141, 1582, 1802 |
| MaeI | CTAG | 2 cut(s) | 1256, 1589 |
| MaeII | ACGT | 1 cut(s) | 575 |
| MaeIII | GTNAC | 2 cut(s) | 4, 1213 |
| MalI | GATC | 3 cut(s) | 1621, 1652, 1683 |
| MboI | GATC | 3 cut(s) | 1619, 1650, 1681 |
| MflI | RGATCY | 2 cut(s) | 1619, 1681 |
| MhlI | GDGCHC | 2 cut(s) | 197, 435 |
| MluCI | AATT | 7 cut(s) | 62, 149, 598, 1273, 1561, 1605, 1634 |
| MlyI | GAGTC | 4 cut(s) | 321, 405, 575, 940 |
| MmeI | TCCRAC | 1 cut(s) | 543 |
| Mph1103I | ATGCAT | 1 cut(s) | 1597 |
| MroI | TCCGGA | 1 cut(s) | 1684 |
| MroXI | GAANNNNTTC | 1 cut(s) | 575 |
| MseI | TTAA | 3 cut(s) | 1476, 1485, 1610 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1659 |
| MspA1I | CMGCKG | 1 cut(s) | 538 |
| MspI | CCGG | 4 cut(s) | 352, 402, 691, 1685 |
| MspR9I | CCNGG | 5 cut(s) | 353, 402, 611, 878, 1773 |
| MvaI | CCWGG | 3 cut(s) | 611, 878, 1773 |
| MvnI | CGCG | 4 cut(s) | 378, 504, 586, 588 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 354, 370, 390, 878, 1320, 1373, 1520 |
| NciI | CCSGG | 2 cut(s) | 353, 402 |
| NdeII | GATC | 3 cut(s) | 1619, 1650, 1681 |
| NlaIII | CATG | 3 cut(s) | 913, 1519, 1658 |
| NlaIV | GGNNCC | 5 cut(s) | 178, 196, 356, 608, 689 |
| NmeAIII | GCCGAG | 2 cut(s) | 419, 454 |
| NmuCI | GTSAC | 2 cut(s) | 4, 1213 |
| NsiI | ATGCAT | 1 cut(s) | 1597 |
| PaeR7I | CTCGAG | 1 cut(s) | 338 |
| PaqCI | CACCTGC | 1 cut(s) | 369 |
| PciSI | GCTCTTC | 1 cut(s) | 20 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 246, 426, 516, 528 |
| PdmI | GAANNNNTTC | 1 cut(s) | 575 |
| PfeI | GAWTC | 7 cut(s) | 99, 1102, 1126, 1201, 1414, 1665, 1713 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1838 |
| PkrI | GCNGC | 7 cut(s) | 304, 347, 503, 537, 1182, 1378, 1579 |
| PleI | GAGTC | 4 cut(s) | 320, 404, 575, 940 |
| PpsI | GAGTC | 4 cut(s) | 320, 404, 575, 940 |
| Psp124BI | GAGCTC | 1 cut(s) | 435 |
| Psp6I | CCWGG | 3 cut(s) | 609, 876, 1771 |
| PspEI | GGTNACC | 1 cut(s) | 4 |
| PspFI | CCCAGC | 2 cut(s) | 769, 1196 |
| PspGI | CCWGG | 3 cut(s) | 609, 876, 1771 |
| PspN4I | GGNNCC | 5 cut(s) | 178, 196, 356, 608, 689 |
| PspPI | GGNCC | 3 cut(s) | 176, 355, 1358 |
| PstI | CTGCAG | 2 cut(s) | 1378, 1582 |
| PsuI | RGATCY | 2 cut(s) | 1619, 1681 |
| RsaI | GTAC | 3 cut(s) | 509, 554, 1268 |
| RsaNI | GTAC | 3 cut(s) | 508, 553, 1267 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1659 |
| SacI | GAGCTC | 1 cut(s) | 435 |
| SapI | GCTCTTC | 1 cut(s) | 20 |
| SaqAI | TTAA | 3 cut(s) | 1476, 1485, 1610 |
| SatI | GCNGC | 7 cut(s) | 303, 346, 502, 536, 1181, 1377, 1578 |
| Sau3AI | GATC | 3 cut(s) | 1619, 1650, 1681 |
| Sau96I | GGNCC | 3 cut(s) | 176, 355, 1358 |
| SchI | GAGTC | 4 cut(s) | 321, 405, 575, 940 |
| ScrFI | CCNGG | 5 cut(s) | 353, 402, 611, 878, 1773 |
| SduI | GDGCHC | 2 cut(s) | 197, 435 |
| SfaNI | GCATC | 3 cut(s) | 1141, 1582, 1802 |
| SfcI | CTRYAG | 3 cut(s) | 1168, 1374, 1578 |
| Sfr274I | CTCGAG | 1 cut(s) | 338 |
| SinI | GGWCC | 2 cut(s) | 176, 1358 |
| SlaI | CTCGAG | 1 cut(s) | 338 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1659 |
| SmlI | CTYRAG | 1 cut(s) | 338 |
| SmoI | CTYRAG | 1 cut(s) | 338 |
| Sse9I | AATT | 7 cut(s) | 62, 149, 598, 1273, 1561, 1605, 1634 |
| SspMI | CTAG | 2 cut(s) | 1256, 1589 |
| SstI | GAGCTC | 1 cut(s) | 435 |
| StyD4I | CCNGG | 5 cut(s) | 351, 400, 609, 876, 1771 |
| StyI | CCWWGG | 1 cut(s) | 1623 |
| TaaI | ACNGT | 7 cut(s) | 329, 816, 837, 1033, 1135, 1264, 1722 |
| TaiI | ACGT | 1 cut(s) | 578 |
| TasI | AATT | 7 cut(s) | 62, 149, 598, 1273, 1561, 1605, 1634 |
| TatI | WGTACW | 2 cut(s) | 552, 1266 |
| TauI | GCSGC | 4 cut(s) | 305, 348, 504, 538 |
| TfiI | GAWTC | 7 cut(s) | 99, 1102, 1126, 1201, 1414, 1665, 1713 |
| Tru1I | TTAA | 3 cut(s) | 1476, 1485, 1610 |
| Tru9I | TTAA | 3 cut(s) | 1476, 1485, 1610 |
| TscAI | CASTG | 3 cut(s) | 1269, 1725, 1839 |
| TseFI | GTSAC | 2 cut(s) | 4, 1213 |
| TseI | GCWGC | 3 cut(s) | 1180, 1376, 1577 |
| Tsp45I | GTSAC | 2 cut(s) | 4, 1213 |
| TspDTI | ATGAA | 9 cut(s) | 423, 744, 846, 902, 986, 1080, 1495, 1574, 1644 |
| TspGWI | ACGGA | 1 cut(s) | 668 |
| TspRI | CASTG | 3 cut(s) | 1269, 1725, 1839 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1838 |
| VpaK11BI | GGWCC | 2 cut(s) | 176, 1358 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 188 |
| XapI | RAATTY | 6 cut(s) | 62, 598, 1273, 1561, 1605, 1634 |
| XbaI | TCTAGA | 1 cut(s) | 1588 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1549 |
| XhoI | CTCGAG | 1 cut(s) | 338 |
| XmiI | GTMKAC | 1 cut(s) | 940 |
| XmnI | GAANNNNTTC | 1 cut(s) | 575 |
| XspI | CTAG | 2 cut(s) | 1256, 1589 |
| Zsp2I | ATGCAT | 1 cut(s) | 1597 |