Rmu_sc0000247.1_g000072

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000247.1
OG0011880
Physical Location & Seq
Forward (+)
368328 .. 374907
6580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000247.1_g000072.1.cds

Sequence Viewer

Length: 2028 bp
atgtaccgctcggccatggtgagccagccgacgatgtcaccgccattgaaacgaggcaggttgagttttgggggtctgtacgggtggtggtcatggtgcaagggagggggggcagcgttggttgttcggcggggtgaagtggaaggagttgggaggttgggaagcgagagagggtggtttgtgaggagtgtggaaggggtgggaaggagccaaatgagagctgagtggtgtacggagtggagggaattgggggttcagaggtgtgggtcggaggtggtgacgaaggggtgtgctggcggagggggcaatggtgggatagccttgagccagggaggtcgtcatgttgcgattgcagtgtttcgttttgggtttggagcatatccacggtggcttgaaaagttgcgatttgaaagcttcagtagggttttcaacatcgccgccctccgagtccccgcaaaagactgttttgccctcgagagccgcctccggggccacctgctgaactggcctcgcgtccgcaacatcggccgagtccccggcgacgaagtcgaaggcgacctcgccgagctcctcgctaataatagtaatcaaagcaacgaagaggaagaccaaaacgacgtcgtttcgctgaaccgccgcgtgtacgggaaatccgacggcgacggcgagccgctgagtgcggtgctgtacagagagaagctggcgaagacgttcgactcgcgcgggtatgtgaattttcggaacctggcgaagatgtcgaggccgaaccagaagaagaggagggtgacggaggaagataacggcggtgaagggaggaagaggaaccggaagggtgagttttcggtgctggaggttgtggcggatgaagaagaagaagaaggagaggacttgaagggtttgctgggggatgagtttcaggggaggaagagatggaggggttcgacgcggttgttgctcttggatgaacggtatggggataaaggcgtggaggagttgcctcaggcaatccaggctgtgatgaaggaaaatacctcgaaaagcatggggcctgctattgagcttgctgtgatgaaggaaaatacctcgaaaagcatggggcctgctattgagcttgtgagatgcagactgactcttttgtatgattactggcagatgaatgagatattggaggctttgctccccgaacgtatgattgttccttcggcctttgaaacagtcggccatattgcacacctaaacctgagagatgaacatttaccttacagaaagtttatagctaaggtgattcttgataaaaataagccaaagattcagacagttgtaaataaaatcgatgccattcaaaatgactatagaacaatgcagcttgaagttttagctgggaatcattcgcttgtgacaaaagtagttgagaatggtttgcgatttcaggttgacctagcaacagtgtactggaattccagacttgcgacagaaagacaaaggctattgaacagctttacccgcaatgatgttgtgtgtgatgttttctctggagttggtccaatagctatctctgcagcaaagattgtaaaacgagtttatgccaatgatttgaatccccacgctgttgaatatctgcaaagaaatagtggtatcaacaaactcgataggaagattaaggtctttaatatggatggaaggaggttcattagcgccatgtttgcaagtgataaagctaaatccattacacaagtggtgatgaatttgccaaaggacgctgcagagtatctagatgcatttcggggaattttaagagatagatccaaggatgaagaatttagcttgccaatgatccatgtttatggattctccaaagctgaagatccggagtttgacttccatcagagaataagaatcgcactgtcagaagtggcagttgatgtagaaatgcgtagggttcgccttgttgcaccagggaaatggatgttatgtgcatcatttatcctcccaaagagtatagctttttcaaaaccagtggatgtataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_10

Protein Analysis

675

Amino Acids

76.17

Weight (kDa)

9.27

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27340 AT4G27340 AT4G27340
fragaria_vesca FvH4_3g21210
malus_domestica MD03G1217000.v1.1
prunus_persica Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1
pyrus_communis pycom03g16620
rosa_chinensis RchiOBHm_Chr5g0036281
rosa_laevigata RLG00000033683
rosa_multiflora Rmu_sc0000247.1_g000072
rosa_roxburghii Rroxscaffold_1G00044510
rosa_rugosa Rorug05G0154500
rosa_samantha Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500
rosa_wichuraiana Rw5G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 504
AatII GACGTC 1 cut(s) 621
Acc36I ACCTGC 2 cut(s) 48, 504
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 5 cut(s) 513, 639, 721, 723, 946
AccIII TCCGGA 1 cut(s) 1867
AclWI GGATC 3 cut(s) 1797, 1828, 1859
AcoI YGGCCR 3 cut(s) 12, 526, 1219
AcsI RAATTY 5 cut(s) 733, 1456, 1744, 1788, 1817
AcuI CTGAAG 2 cut(s) 400, 1881
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 6 cut(s) 5, 80, 232, 644, 689, 1451
AfiI CCNNNNNNNGG 1 cut(s) 487
AjnI CCWGG 4 cut(s) 327, 744, 1008, 1954
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
Alw21I GWGCWC 1 cut(s) 570
AlwI GGATC 3 cut(s) 1797, 1828, 1859
Ama87I CYCGRG 1 cut(s) 473
Aor13HI TCCGGA 1 cut(s) 1867
AoxI GGCC 9 cut(s) 12, 490, 506, 526, 761, 1046, 1097, 1203, 1219
ApeKI GCWGC 4 cut(s) 113, 1363, 1559, 1760
ApoI RAATTY 5 cut(s) 733, 1456, 1744, 1788, 1817
Asp700I GAANNNNTTC 1 cut(s) 710
AspLEI GCGC 2 cut(s) 723, 1697
AspS9I GGNCC 4 cut(s) 490, 1046, 1097, 1541
AsuC2I CCSGG 2 cut(s) 488, 537
AsuHPI GGTGA 9 cut(s) 30, 31, 146, 289, 796, 818, 845, 1294, 1750
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 1541
AxyI CCTNAGG 1 cut(s) 999
BanII GRGCYC 1 cut(s) 570
BbsI GAAGAC 2 cut(s) 612, 713
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 4 cut(s) 125, 1375, 1571, 1747
BccI CCATC 3 cut(s) 924, 1670, 1890
BceAI ACGGC 3 cut(s) 673, 679, 817
BciT130I CCWGG 4 cut(s) 329, 746, 1010, 1956
BcnI CCSGG 2 cut(s) 488, 537
BfaI CTAG 2 cut(s) 1439, 1772
BfmI CTRYAG 3 cut(s) 1351, 1557, 1761
BfoI RGCGCY 1 cut(s) 1698
BfuAI ACCTGC 2 cut(s) 48, 504
BglI GCCNNNNNGGC 1 cut(s) 489
BisI GCNGC 8 cut(s) 114, 438, 481, 637, 671, 1364, 1560, 1761
BlsI GCNGC 8 cut(s) 115, 439, 482, 638, 672, 1365, 1561, 1762
Bme1390I CCNGG 6 cut(s) 329, 488, 537, 746, 1010, 1956
Bme18I GGWCC 1 cut(s) 1541
BmeT110I CYCGRG 1 cut(s) 473
BmgT120I GGNCC 4 cut(s) 490, 1046, 1097, 1541
BmiI GGNNCC 6 cut(s) 209, 491, 743, 824, 1047, 1098
BmrFI CCNGG 6 cut(s) 329, 488, 537, 746, 1010, 1956
BmsI GCATC 4 cut(s) 1109, 1324, 1765, 1985
BpiI GAAGAC 2 cut(s) 612, 713
BpmI CTGGAG 2 cut(s) 869, 1554
Bpu10I CCTNAGC 1 cut(s) 1278
BpuEI CTTGAG 1 cut(s) 343
BpuMI CCSGG 2 cut(s) 488, 537
Bsa29I ATCGAT 1 cut(s) 1332
BsaBI GATNNNNATC 1 cut(s) 1596
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 7 cut(s) 15, 328, 383, 487, 535, 1806, 1955
BsaWI WCCGGW 2 cut(s) 825, 1867
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse1I ACTGG 4 cut(s) 509, 1151, 1457, 2015
Bse21I CCTNAGG 1 cut(s) 999
Bse3DI GCAATG 2 cut(s) 313, 1513
Bse8I GATNNNNATC 1 cut(s) 1596
BseAI TCCGGA 1 cut(s) 1867
BseBI CCWGG 4 cut(s) 329, 746, 1010, 1956
BseCI ATCGAT 1 cut(s) 1332
BseDI CCNNGG 7 cut(s) 15, 328, 383, 487, 535, 1806, 1955
BseGI GGATG 7 cut(s) 868, 913, 967, 1681, 1816, 1971, 2026
BseJI GATNNNNATC 1 cut(s) 1596
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMI GCAATG 2 cut(s) 313, 1513
BseMII CTCAG 4 cut(s) 213, 665, 1013, 1232
BseNI ACTGG 4 cut(s) 509, 1151, 1457, 2015
BseRI GAGGAG 4 cut(s) 199, 560, 793, 1004
BseX3I CGGCCG 1 cut(s) 526
BseXI GCAGC 4 cut(s) 125, 1375, 1571, 1747
BseYI CCCAGC 2 cut(s) 901, 1379
Bsh1236I CGCG 5 cut(s) 513, 639, 721, 723, 946
Bsh1285I CGRYCG 1 cut(s) 529
BshFI GGCC 9 cut(s) 14, 492, 508, 528, 763, 1048, 1099, 1205, 1221
BshVI ATCGAT 1 cut(s) 1332
BsiEI CGRYCG 1 cut(s) 529
BsiHKAI GWGCWC 1 cut(s) 570
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 4 cut(s) 487, 537, 826, 1868
BslFI GGGAC 2 cut(s) 434, 518
BslI CCNNNNNNNGG 1 cut(s) 487
BsmFI GGGAC 2 cut(s) 434, 518
BsnI GGCC 9 cut(s) 14, 492, 508, 528, 763, 1048, 1099, 1205, 1221
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 570
Bsp13I TCCGGA 1 cut(s) 1867
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 1802, 1833, 1864
Bsp19I CCATGG 1 cut(s) 15
BspANI GGCC 9 cut(s) 14, 492, 508, 528, 763, 1048, 1099, 1205, 1221
BspCNI CTCAG 4 cut(s) 214, 666, 1012, 1233
BspDI ATCGAT 1 cut(s) 1332
BspEI TCCGGA 1 cut(s) 1867
BspFNI CGCG 5 cut(s) 513, 639, 721, 723, 946
BspLI GGNNCC 6 cut(s) 209, 491, 743, 824, 1047, 1098
BspMAI CTGCAG 2 cut(s) 1561, 1765
BspMI ACCTGC 2 cut(s) 48, 504
BspPI GGATC 3 cut(s) 1797, 1828, 1859
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 2 cut(s) 313, 1513
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 4 cut(s) 509, 1151, 1457, 2015
BssECI CCNNGG 7 cut(s) 15, 328, 383, 487, 535, 1806, 1955
BssMI GATC 3 cut(s) 1802, 1833, 1864
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 15, 1806
Bst2UI CCWGG 4 cut(s) 329, 746, 1010, 1956
Bst4CI ACNGT 7 cut(s) 387, 464, 969, 1216, 1318, 1447, 1905
Bst6I CTCTTC 4 cut(s) 594, 770, 812, 920
BstACI GRCGYC 1 cut(s) 618
BstAUI TGTACA 1 cut(s) 687
BstC8I GCNNGC 8 cut(s) 26, 295, 668, 702, 1050, 1062, 1101, 1826
BstDEI CTNAG 5 cut(s) 222, 674, 999, 1241, 1278
BstDSI CCRYGG 2 cut(s) 15, 383
BstF5I GGATG 7 cut(s) 868, 913, 967, 1681, 1816, 1971, 2026
BstFNI CGCG 5 cut(s) 513, 639, 721, 723, 946
BstH2I RGCGCY 1 cut(s) 1698
BstHHI GCGC 2 cut(s) 723, 1697
BstKTI GATC 3 cut(s) 1805, 1836, 1867
BstMBI GATC 3 cut(s) 1802, 1833, 1864
BstMCI CGRYCG 1 cut(s) 529
BstMWI GCNNNNNNNGC 9 cut(s) 303, 489, 505, 525, 952, 1010, 1503, 1556, 1703
BstNI CCWGG 4 cut(s) 329, 746, 1010, 1956
BstSCI CCNGG 6 cut(s) 327, 486, 535, 744, 1008, 1954
BstSFI CTRYAG 3 cut(s) 1351, 1557, 1761
BstUI CGCG 5 cut(s) 513, 639, 721, 723, 946
BstV1I GCAGC 4 cut(s) 125, 1375, 1571, 1747
BstV2I GAAGAC 2 cut(s) 612, 713
BstX2I RGATCY 2 cut(s) 1802, 1864
BstXI CCANNNNNNTGG 2 cut(s) 1844, 1962
BstYI RGATCY 2 cut(s) 1802, 1864
BstZI CGGCCG 1 cut(s) 526
Bsu15I ATCGAT 1 cut(s) 1332
Bsu36I CCTNAGG 1 cut(s) 999
BsuRI GGCC 9 cut(s) 14, 492, 508, 528, 763, 1048, 1099, 1205, 1221
BsuTUI ATCGAT 1 cut(s) 1332
BtgI CCRYGG 2 cut(s) 15, 383
BtgZI GCGATG 1 cut(s) 418
BtsCI GGATG 7 cut(s) 868, 913, 967, 1681, 1816, 1971, 2026
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 4 cut(s) 360, 1452, 1901, 2022
BveI ACCTGC 2 cut(s) 48, 504
Cac8I GCNNGC 8 cut(s) 26, 295, 668, 702, 1050, 1062, 1101, 1826
CfoI GCGC 2 cut(s) 723, 1697
Cfr13I GGNCC 4 cut(s) 490, 1046, 1097, 1541
ClaI ATCGAT 1 cut(s) 1332
CseI GACGC 3 cut(s) 502, 952, 1766
Csp6I GTAC 6 cut(s) 4, 79, 231, 643, 688, 1450
CspCI CAANNNNNGTGG 1 cut(s) 1998
CviAII CATG 7 cut(s) 16, 93, 341, 1042, 1093, 1699, 1838
CviQI GTAC 6 cut(s) 4, 79, 231, 643, 688, 1450
DdeI CTNAG 5 cut(s) 222, 674, 999, 1241, 1278
DpnI GATC 3 cut(s) 1804, 1835, 1866
DpnII GATC 3 cut(s) 1802, 1833, 1864
EaeI YGGCCR 3 cut(s) 12, 526, 1219
EagI CGGCCG 1 cut(s) 526
Eam1104I CTCTTC 4 cut(s) 594, 770, 812, 920
EarI CTCTTC 4 cut(s) 594, 770, 812, 920
EciI GGCGGA 2 cut(s) 312, 875
Ecl136II GAGCTC 1 cut(s) 568
EclXI CGGCCG 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 15, 1806
Eco24I GRGCYC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 1541
Eco52I CGGCCG 1 cut(s) 526
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 2 cut(s) 400, 1881
Eco81I CCTNAGG 1 cut(s) 999
Eco88I CYCGRG 1 cut(s) 473
EcoICRI GAGCTC 1 cut(s) 568
EcoO109I RGGNCCY 2 cut(s) 1046, 1097
EcoRI GAATTC 1 cut(s) 1456
EcoRII CCWGG 4 cut(s) 327, 744, 1008, 1954
EcoT14I CCWWGG 2 cut(s) 15, 1806
EcoT22I ATGCAT 1 cut(s) 1780
EcoT38I GRGCYC 1 cut(s) 570
ErhI CCWWGG 2 cut(s) 15, 1806
FaeI CATG 7 cut(s) 19, 96, 344, 1045, 1096, 1702, 1841
FaqI GGGAC 2 cut(s) 434, 518
FatI CATG 7 cut(s) 15, 92, 340, 1041, 1092, 1698, 1837
FauI CCCGC 4 cut(s) 123, 460, 716, 1511
Fnu4HI GCNGC 8 cut(s) 114, 438, 481, 637, 671, 1364, 1560, 1761
FokI GGATG 6 cut(s) 875, 920, 974, 1688, 1823, 1978
FriOI GRGCYC 1 cut(s) 570
Fsp4HI GCNGC 8 cut(s) 114, 438, 481, 637, 671, 1364, 1560, 1761
FspBI CTAG 2 cut(s) 1439, 1772
GlaI GCGC 2 cut(s) 722, 1696
GluI GCNGC 8 cut(s) 114, 438, 481, 637, 671, 1364, 1560, 1761
GsaI CCCAGC 2 cut(s) 905, 1383
GsuI CTGGAG 2 cut(s) 869, 1554
HaeII RGCGCY 1 cut(s) 1698
HaeIII GGCC 9 cut(s) 14, 492, 508, 528, 763, 1048, 1099, 1205, 1221
HapII CCGG 4 cut(s) 487, 537, 826, 1868
HgaI GACGC 3 cut(s) 502, 952, 1766
HhaI GCGC 2 cut(s) 723, 1697
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 7 cut(s) 19, 96, 344, 1045, 1096, 1702, 1841
Hin6I GCGC 2 cut(s) 721, 1695
HinP1I GCGC 2 cut(s) 721, 1695
HincII GTYRAC 1 cut(s) 1435
HindII GTYRAC 1 cut(s) 1435
HindIII AAGCTT 1 cut(s) 412
HpaII CCGG 4 cut(s) 487, 537, 826, 1868
HphI GGTGA 9 cut(s) 30, 31, 146, 289, 796, 818, 845, 1294, 1750
Hpy166II GTNNAC 4 cut(s) 231, 643, 1435, 1450
Hpy188I TCNGA 8 cut(s) 258, 271, 446, 655, 741, 1314, 1887, 1909
Hpy188III TCNNGA 6 cut(s) 475, 1289, 1461, 1533, 1772, 1868
Hpy8I GTNNAC 4 cut(s) 231, 643, 1435, 1450
Hpy99I CGWCG 7 cut(s) 34, 545, 620, 623, 659, 665, 946
HpyCH4III ACNGT 7 cut(s) 387, 464, 969, 1216, 1318, 1447, 1905
HpyCH4IV ACGT 3 cut(s) 618, 710, 1186
HpyF10VI GCNNNNNNNGC 9 cut(s) 303, 489, 505, 525, 952, 1010, 1503, 1556, 1703
HpyF3I CTNAG 5 cut(s) 222, 674, 999, 1241, 1278
HpySE526I ACGT 3 cut(s) 618, 710, 1186
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 7 cut(s) 19, 96, 344, 1045, 1096, 1702, 1841
HspAI GCGC 2 cut(s) 721, 1695
Kpn2I TCCGGA 1 cut(s) 1867
Kzo9I GATC 3 cut(s) 1802, 1833, 1864
LmnI GCTCC 4 cut(s) 207, 374, 573, 1182
Lsp1109I GCAGC 4 cut(s) 125, 1375, 1571, 1747
LweI GCATC 4 cut(s) 1109, 1324, 1765, 1985
MaeI CTAG 2 cut(s) 1439, 1772
MaeII ACGT 3 cut(s) 618, 710, 1186
MaeIII GTNAC 4 cut(s) 36, 277, 784, 1396
MalI GATC 3 cut(s) 1804, 1835, 1866
MbiI CCGCTC 1 cut(s) 9
MboI GATC 3 cut(s) 1802, 1833, 1864
MflI RGATCY 2 cut(s) 1802, 1864
MhlI GDGCHC 1 cut(s) 570
MluCI AATT 6 cut(s) 245, 733, 1456, 1744, 1788, 1817
MlyI GAGTC 4 cut(s) 456, 540, 710, 1123
MmeI TCCRAC 2 cut(s) 249, 678
Mph1103I ATGCAT 1 cut(s) 1780
MroI TCCGGA 1 cut(s) 1867
MroXI GAANNNNTTC 1 cut(s) 710
MseI TTAA 3 cut(s) 1659, 1668, 1793
MslI CAYNNNNRTG 1 cut(s) 1842
MspA1I CMGCKG 1 cut(s) 673
MspI CCGG 4 cut(s) 487, 537, 826, 1868
MspR9I CCNGG 6 cut(s) 329, 488, 537, 746, 1010, 1956
MvaI CCWGG 4 cut(s) 329, 746, 1010, 1956
MvnI CGCG 5 cut(s) 513, 639, 721, 723, 946
MwoI GCNNNNNNNGC 9 cut(s) 303, 489, 505, 525, 952, 1010, 1503, 1556, 1703
NciI CCSGG 2 cut(s) 488, 537
NcoI CCATGG 1 cut(s) 15
NdeII GATC 3 cut(s) 1802, 1833, 1864
NlaIII CATG 7 cut(s) 19, 96, 344, 1045, 1096, 1702, 1841
NlaIV GGNNCC 6 cut(s) 209, 491, 743, 824, 1047, 1098
NmeAIII GCCGAG 2 cut(s) 554, 589
NmuCI GTSAC 4 cut(s) 36, 277, 784, 1396
NsiI ATGCAT 1 cut(s) 1780
PaeR7I CTCGAG 1 cut(s) 473
PaqCI CACCTGC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 2 cut(s) 651, 663
PdmI GAANNNNTTC 1 cut(s) 710
PfeI GAWTC 6 cut(s) 1285, 1309, 1384, 1597, 1848, 1896
PflFI GACNNNGTC 2 cut(s) 34, 545
PkrI GCNGC 8 cut(s) 115, 439, 482, 638, 672, 1365, 1561, 1762
PleI GAGTC 4 cut(s) 455, 539, 710, 1123
PpsI GAGTC 4 cut(s) 455, 539, 710, 1123
Psp124BI GAGCTC 1 cut(s) 570
Psp6I CCWGG 4 cut(s) 327, 744, 1008, 1954
PspFI CCCAGC 2 cut(s) 901, 1379
PspGI CCWGG 4 cut(s) 327, 744, 1008, 1954
PspN4I GGNNCC 6 cut(s) 209, 491, 743, 824, 1047, 1098
PspPI GGNCC 4 cut(s) 490, 1046, 1097, 1541
PstI CTGCAG 2 cut(s) 1561, 1765
PsuI RGATCY 2 cut(s) 1802, 1864
PsyI GACNNNGTC 2 cut(s) 34, 545
RsaI GTAC 6 cut(s) 5, 80, 232, 644, 689, 1451
RsaNI GTAC 6 cut(s) 4, 79, 231, 643, 688, 1450
RseI CAYNNNNRTG 1 cut(s) 1842
SacI GAGCTC 1 cut(s) 570
SaqAI TTAA 3 cut(s) 1659, 1668, 1793
SatI GCNGC 8 cut(s) 114, 438, 481, 637, 671, 1364, 1560, 1761
Sau3AI GATC 3 cut(s) 1802, 1833, 1864
Sau96I GGNCC 4 cut(s) 490, 1046, 1097, 1541
SchI GAGTC 4 cut(s) 456, 540, 710, 1123
ScrFI CCNGG 6 cut(s) 329, 488, 537, 746, 1010, 1956
SduI GDGCHC 1 cut(s) 570
SfaNI GCATC 4 cut(s) 1109, 1324, 1765, 1985
SfcI CTRYAG 3 cut(s) 1351, 1557, 1761
Sfr274I CTCGAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 1541
SlaI CTCGAG 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 1842
SmlI CTYRAG 2 cut(s) 322, 473
SmoI CTYRAG 2 cut(s) 322, 473
Sse9I AATT 6 cut(s) 245, 733, 1456, 1744, 1788, 1817
SspMI CTAG 2 cut(s) 1439, 1772
SstI GAGCTC 1 cut(s) 570
StyD4I CCNGG 6 cut(s) 327, 486, 535, 744, 1008, 1954
StyI CCWWGG 2 cut(s) 15, 1806
TaaI ACNGT 7 cut(s) 387, 464, 969, 1216, 1318, 1447, 1905
TaiI ACGT 3 cut(s) 621, 713, 1189
TaqI TCGA 9 cut(s) 474, 549, 714, 758, 941, 1034, 1085, 1332, 1647
TasI AATT 6 cut(s) 245, 733, 1456, 1744, 1788, 1817
TatI WGTACW 2 cut(s) 687, 1449
TauI GCSGC 4 cut(s) 440, 483, 639, 673
TfiI GAWTC 6 cut(s) 1285, 1309, 1384, 1597, 1848, 1896
Tru1I TTAA 3 cut(s) 1659, 1668, 1793
Tru9I TTAA 3 cut(s) 1659, 1668, 1793
TscAI CASTG 4 cut(s) 360, 1452, 1908, 2022
TseFI GTSAC 4 cut(s) 36, 277, 784, 1396
TseI GCWGC 4 cut(s) 113, 1363, 1559, 1760
Tsp45I GTSAC 4 cut(s) 36, 277, 784, 1396
TspDTI ATGAA 9 cut(s) 879, 978, 1034, 1085, 1169, 1263, 1678, 1757, 1827
TspGWI ACGGA 2 cut(s) 248, 803
TspRI CASTG 4 cut(s) 360, 1452, 1908, 2022
Tth111I GACNNNGTC 2 cut(s) 34, 545
VpaK11BI GGWCC 1 cut(s) 1541
XapI RAATTY 5 cut(s) 733, 1456, 1744, 1788, 1817
XbaI TCTAGA 1 cut(s) 1771
XcmI CCANNNNNNNNNTGG 1 cut(s) 1732
XhoI CTCGAG 1 cut(s) 473
XmnI GAANNNNTTC 1 cut(s) 710
XspI CTAG 2 cut(s) 1439, 1772
ZraI GACGTC 1 cut(s) 619
Zsp2I ATGCAT 1 cut(s) 1780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.