Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 396 |
| Acc36I | ACCTGC | 1 cut(s) | 396 |
| AccB1I | GGYRCC | 2 cut(s) | 71, 222 |
| AccII | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| AccIII | TCCGGA | 1 cut(s) | 1702 |
| AclWI | GGATC | 3 cut(s) | 1632, 1663, 1694 |
| AcoI | YGGCCR | 3 cut(s) | 3, 418, 1054 |
| AcsI | RAATTY | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| AcuI | CTGAAG | 2 cut(s) | 292, 1716 |
| AcyI | GRCGYC | 1 cut(s) | 507 |
| AfaI | GTAC | 4 cut(s) | 42, 533, 578, 1286 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 81, 130, 379 |
| AgsI | TTSAA | 9 cut(s) | 50, 322, 778, 1046, 1178, 1205, 1327, 1432, 1448 |
| AjnI | CCWGG | 3 cut(s) | 633, 894, 1789 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 227, 259, 983, 1015 |
| Alw21I | GWGCWC | 1 cut(s) | 462 |
| AlwI | GGATC | 3 cut(s) | 1632, 1663, 1694 |
| Ama87I | CYCGRG | 1 cut(s) | 365 |
| Aor13HI | TCCGGA | 1 cut(s) | 1702 |
| AoxI | GGCC | 8 cut(s) | 3, 382, 398, 418, 650, 932, 1038, 1054 |
| ApeKI | GCWGC | 3 cut(s) | 1198, 1394, 1595 |
| ApoI | RAATTY | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 599 |
| AspLEI | GCGC | 2 cut(s) | 612, 1532 |
| AspS9I | GGNCC | 3 cut(s) | 382, 932, 1376 |
| AsuC2I | CCSGG | 3 cut(s) | 83, 380, 429 |
| AsuHPI | GGTGA | 5 cut(s) | 685, 707, 734, 1129, 1585 |
| AvaI | CYCGRG | 1 cut(s) | 365 |
| AvaII | GGWCC | 1 cut(s) | 1376 |
| AxyI | CCTNAGG | 1 cut(s) | 885 |
| BaeGI | GKGCMC | 1 cut(s) | 225 |
| BalI | TGGCCA | 2 cut(s) | 5, 1056 |
| BanI | GGYRCC | 2 cut(s) | 71, 222 |
| BanII | GRGCYC | 1 cut(s) | 462 |
| BbsI | GAAGAC | 3 cut(s) | 450, 501, 602 |
| Bbv12I | GWGCWC | 1 cut(s) | 462 |
| BbvI | GCAGC | 3 cut(s) | 1210, 1406, 1582 |
| BccI | CCATC | 7 cut(s) | 14, 80, 84, 161, 810, 1505, 1725 |
| BceAI | ACGGC | 3 cut(s) | 562, 568, 706 |
| BciT130I | CCWGG | 3 cut(s) | 635, 896, 1791 |
| BcnI | CCSGG | 3 cut(s) | 83, 380, 429 |
| BfaI | CTAG | 2 cut(s) | 1274, 1607 |
| BfmI | CTRYAG | 3 cut(s) | 1186, 1392, 1596 |
| BfoI | RGCGCY | 1 cut(s) | 1533 |
| BfuAI | ACCTGC | 1 cut(s) | 396 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 381 |
| BisI | GCNGC | 8 cut(s) | 330, 373, 421, 526, 560, 1199, 1395, 1596 |
| BlsI | GCNGC | 8 cut(s) | 331, 374, 422, 527, 561, 1200, 1396, 1597 |
| Bme1390I | CCNGG | 6 cut(s) | 83, 380, 429, 635, 896, 1791 |
| Bme18I | GGWCC | 1 cut(s) | 1376 |
| BmeT110I | CYCGRG | 1 cut(s) | 365 |
| BmgT120I | GGNCC | 3 cut(s) | 382, 932, 1376 |
| BmiI | GGNNCC | 7 cut(s) | 27, 73, 224, 383, 632, 713, 933 |
| BmrFI | CCNGG | 6 cut(s) | 83, 380, 429, 635, 896, 1791 |
| BmrI | ACTGGG | 1 cut(s) | 119 |
| BmsI | GCATC | 3 cut(s) | 944, 1159, 1820 |
| BmuI | ACTGGG | 1 cut(s) | 119 |
| BpiI | GAAGAC | 3 cut(s) | 450, 501, 602 |
| BpmI | CTGGAG | 1 cut(s) | 758 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1113 |
| BpuMI | CCSGG | 3 cut(s) | 83, 380, 429 |
| Bsa29I | ATCGAT | 1 cut(s) | 1167 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1431 |
| BsaHI | GRCGYC | 1 cut(s) | 507 |
| BsaJI | CCNNGG | 4 cut(s) | 379, 427, 1641, 1790 |
| BsaWI | WCCGGW | 2 cut(s) | 714, 1702 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 81, 130, 379 |
| Bse1I | ACTGG | 5 cut(s) | 125, 401, 986, 1292, 1850 |
| Bse21I | CCTNAGG | 1 cut(s) | 885 |
| Bse3DI | GCAATG | 1 cut(s) | 1348 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1431 |
| BseAI | TCCGGA | 1 cut(s) | 1702 |
| BseBI | CCWGG | 3 cut(s) | 635, 896, 1791 |
| BseCI | ATCGAT | 1 cut(s) | 1167 |
| BseDI | CCNNGG | 4 cut(s) | 379, 427, 1641, 1790 |
| BseJI | GATNNNNATC | 1 cut(s) | 1431 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 81, 130, 379 |
| BseMI | GCAATG | 1 cut(s) | 1348 |
| BseMII | CTCAG | 3 cut(s) | 554, 899, 1380 |
| BseNI | ACTGG | 5 cut(s) | 125, 401, 986, 1292, 1850 |
| BseRI | GAGGAG | 4 cut(s) | 452, 682, 780, 890 |
| BseSI | GKGCMC | 1 cut(s) | 225 |
| BseX3I | CGGCCG | 1 cut(s) | 418 |
| BseXI | GCAGC | 3 cut(s) | 1210, 1406, 1582 |
| BseYI | CCCAGC | 2 cut(s) | 787, 1214 |
| Bsh1236I | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| Bsh1285I | CGRYCG | 1 cut(s) | 421 |
| BshFI | GGCC | 8 cut(s) | 5, 384, 400, 420, 652, 934, 1040, 1056 |
| BshNI | GGYRCC | 2 cut(s) | 71, 222 |
| BshVI | ATCGAT | 1 cut(s) | 1167 |
| BsiEI | CGRYCG | 1 cut(s) | 421 |
| BsiHKAI | GWGCWC | 1 cut(s) | 462 |
| BsiHKCI | CYCGRG | 1 cut(s) | 365 |
| BsiSI | CCGG | 6 cut(s) | 75, 82, 379, 429, 715, 1703 |
| BslFI | GGGAC | 2 cut(s) | 326, 410 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 81, 130, 379 |
| BsmFI | GGGAC | 2 cut(s) | 326, 410 |
| BsnI | GGCC | 8 cut(s) | 5, 384, 400, 420, 652, 934, 1040, 1056 |
| BsoBI | CYCGRG | 1 cut(s) | 365 |
| Bsp1286I | GDGCHC | 2 cut(s) | 225, 462 |
| Bsp13I | TCCGGA | 1 cut(s) | 1702 |
| Bsp1407I | TGTACA | 1 cut(s) | 576 |
| Bsp143I | GATC | 3 cut(s) | 1637, 1668, 1699 |
| BspANI | GGCC | 8 cut(s) | 5, 384, 400, 420, 652, 934, 1040, 1056 |
| BspCNI | CTCAG | 3 cut(s) | 555, 898, 1379 |
| BspDI | ATCGAT | 1 cut(s) | 1167 |
| BspEI | TCCGGA | 1 cut(s) | 1702 |
| BspFNI | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| BspLI | GGNNCC | 7 cut(s) | 27, 73, 224, 383, 632, 713, 933 |
| BspMAI | CTGCAG | 2 cut(s) | 1396, 1600 |
| BspMI | ACCTGC | 1 cut(s) | 396 |
| BspPI | GGATC | 3 cut(s) | 1632, 1663, 1694 |
| BspT107I | GGYRCC | 2 cut(s) | 71, 222 |
| BsrDI | GCAATG | 1 cut(s) | 1348 |
| BsrGI | TGTACA | 1 cut(s) | 576 |
| BsrI | ACTGG | 5 cut(s) | 125, 401, 986, 1292, 1850 |
| BssECI | CCNNGG | 4 cut(s) | 379, 427, 1641, 1790 |
| BssMI | GATC | 3 cut(s) | 1637, 1668, 1699 |
| BssNI | GRCGYC | 1 cut(s) | 507 |
| BssT1I | CCWWGG | 1 cut(s) | 1641 |
| Bst2UI | CCWGG | 3 cut(s) | 635, 896, 1791 |
| Bst4CI | ACNGT | 6 cut(s) | 356, 855, 1051, 1153, 1282, 1740 |
| Bst6I | CTCTTC | 3 cut(s) | 659, 701, 806 |
| BstACI | GRCGYC | 1 cut(s) | 507 |
| BstAUI | TGTACA | 1 cut(s) | 576 |
| BstC8I | GCNNGC | 4 cut(s) | 557, 591, 936, 1661 |
| BstDEI | CTNAG | 4 cut(s) | 563, 885, 1113, 1366 |
| BstFNI | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| BstH2I | RGCGCY | 1 cut(s) | 1533 |
| BstHHI | GCGC | 2 cut(s) | 612, 1532 |
| BstKTI | GATC | 3 cut(s) | 1640, 1671, 1702 |
| BstMBI | GATC | 3 cut(s) | 1637, 1668, 1699 |
| BstMCI | CGRYCG | 1 cut(s) | 421 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 381, 397, 417, 429, 838, 896, 1338, 1391, 1538 |
| BstNI | CCWGG | 3 cut(s) | 635, 896, 1791 |
| BstSCI | CCNGG | 6 cut(s) | 81, 378, 427, 633, 894, 1789 |
| BstSFI | CTRYAG | 3 cut(s) | 1186, 1392, 1596 |
| BstSLI | GKGCMC | 1 cut(s) | 225 |
| BstUI | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| BstV1I | GCAGC | 3 cut(s) | 1210, 1406, 1582 |
| BstV2I | GAAGAC | 3 cut(s) | 450, 501, 602 |
| BstX2I | RGATCY | 2 cut(s) | 1637, 1699 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1679, 1797 |
| BstYI | RGATCY | 2 cut(s) | 1637, 1699 |
| BstZI | CGGCCG | 1 cut(s) | 418 |
| Bsu15I | ATCGAT | 1 cut(s) | 1167 |
| Bsu36I | CCTNAGG | 1 cut(s) | 885 |
| BsuRI | GGCC | 8 cut(s) | 5, 384, 400, 420, 652, 934, 1040, 1056 |
| BsuTUI | ATCGAT | 1 cut(s) | 1167 |
| BtgZI | GCGATG | 1 cut(s) | 310 |
| BtsIMutI | CAGTG | 4 cut(s) | 132, 1287, 1736, 1857 |
| BveI | ACCTGC | 1 cut(s) | 396 |
| Cac8I | GCNNGC | 4 cut(s) | 557, 591, 936, 1661 |
| CfoI | GCGC | 2 cut(s) | 612, 1532 |
| Cfr13I | GGNCC | 3 cut(s) | 382, 932, 1376 |
| ClaI | ATCGAT | 1 cut(s) | 1167 |
| CseI | GACGC | 5 cut(s) | 394, 412, 496, 838, 1601 |
| Csp6I | GTAC | 4 cut(s) | 41, 532, 577, 1285 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 1833 |
| CviAII | CATG | 4 cut(s) | 928, 1534, 1673, 1757 |
| CviQI | GTAC | 4 cut(s) | 41, 532, 577, 1285 |
| DdeI | CTNAG | 4 cut(s) | 563, 885, 1113, 1366 |
| DpnI | GATC | 3 cut(s) | 1639, 1670, 1701 |
| DpnII | GATC | 3 cut(s) | 1637, 1668, 1699 |
| EaeI | YGGCCR | 3 cut(s) | 3, 418, 1054 |
| EagI | CGGCCG | 1 cut(s) | 418 |
| Eam1104I | CTCTTC | 3 cut(s) | 659, 701, 806 |
| EarI | CTCTTC | 3 cut(s) | 659, 701, 806 |
| EciI | GGCGGA | 1 cut(s) | 18 |
| Ecl136II | GAGCTC | 1 cut(s) | 460 |
| EclXI | CGGCCG | 1 cut(s) | 418 |
| Eco130I | CCWWGG | 1 cut(s) | 1641 |
| Eco24I | GRGCYC | 1 cut(s) | 462 |
| Eco47I | GGWCC | 1 cut(s) | 1376 |
| Eco52I | CGGCCG | 1 cut(s) | 418 |
| Eco53kI | GAGCTC | 1 cut(s) | 460 |
| Eco57I | CTGAAG | 2 cut(s) | 292, 1716 |
| Eco81I | CCTNAGG | 1 cut(s) | 885 |
| Eco88I | CYCGRG | 1 cut(s) | 365 |
| EcoICRI | GAGCTC | 1 cut(s) | 460 |
| EcoO109I | RGGNCCY | 1 cut(s) | 932 |
| EcoRI | GAATTC | 1 cut(s) | 1291 |
| EcoRII | CCWGG | 3 cut(s) | 633, 894, 1789 |
| EcoT14I | CCWWGG | 1 cut(s) | 1641 |
| EcoT38I | GRGCYC | 1 cut(s) | 462 |
| ErhI | CCWWGG | 1 cut(s) | 1641 |
| FaeI | CATG | 4 cut(s) | 931, 1537, 1676, 1760 |
| FaqI | GGGAC | 2 cut(s) | 326, 410 |
| FatI | CATG | 4 cut(s) | 927, 1533, 1672, 1756 |
| FauI | CCCGC | 3 cut(s) | 352, 605, 1346 |
| Fnu4HI | GCNGC | 8 cut(s) | 330, 373, 421, 526, 560, 1199, 1395, 1596 |
| FokI | GGATG | 9 cut(s) | 98, 102, 125, 764, 806, 860, 1523, 1658, 1813 |
| FriOI | GRGCYC | 1 cut(s) | 462 |
| Fsp4HI | GCNGC | 8 cut(s) | 330, 373, 421, 526, 560, 1199, 1395, 1596 |
| FspBI | CTAG | 2 cut(s) | 1274, 1607 |
| GlaI | GCGC | 2 cut(s) | 611, 1531 |
| GluI | GCNGC | 8 cut(s) | 330, 373, 421, 526, 560, 1199, 1395, 1596 |
| GsaI | CCCAGC | 2 cut(s) | 791, 1218 |
| GsuI | CTGGAG | 1 cut(s) | 758 |
| HaeII | RGCGCY | 1 cut(s) | 1533 |
| HaeIII | GGCC | 8 cut(s) | 5, 384, 400, 420, 652, 934, 1040, 1056 |
| HapII | CCGG | 6 cut(s) | 75, 82, 379, 429, 715, 1703 |
| HgaI | GACGC | 5 cut(s) | 394, 412, 496, 838, 1601 |
| HhaI | GCGC | 2 cut(s) | 612, 1532 |
| Hin1I | GRCGYC | 1 cut(s) | 507 |
| Hin1II | CATG | 4 cut(s) | 931, 1537, 1676, 1760 |
| Hin6I | GCGC | 2 cut(s) | 610, 1530 |
| HinP1I | GCGC | 2 cut(s) | 610, 1530 |
| HincII | GTYRAC | 1 cut(s) | 1270 |
| HindII | GTYRAC | 1 cut(s) | 1270 |
| HindIII | AAGCTT | 1 cut(s) | 304 |
| HpaII | CCGG | 6 cut(s) | 75, 82, 379, 429, 715, 1703 |
| HphI | GGTGA | 5 cut(s) | 685, 707, 734, 1129, 1585 |
| Hpy166II | GTNNAC | 3 cut(s) | 532, 1270, 1285 |
| Hpy188I | TCNGA | 6 cut(s) | 338, 544, 630, 1149, 1722, 1744 |
| Hpy188III | TCNNGA | 7 cut(s) | 57, 211, 367, 1124, 1296, 1368, 1703 |
| Hpy8I | GTNNAC | 3 cut(s) | 532, 1270, 1285 |
| Hpy99I | CGWCG | 8 cut(s) | 71, 299, 437, 488, 512, 548, 554, 832 |
| HpyCH4III | ACNGT | 6 cut(s) | 356, 855, 1051, 1153, 1282, 1740 |
| HpyCH4IV | ACGT | 1 cut(s) | 599 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 381, 397, 417, 429, 838, 896, 1338, 1391, 1538 |
| HpyF3I | CTNAG | 4 cut(s) | 563, 885, 1113, 1366 |
| HpySE526I | ACGT | 1 cut(s) | 599 |
| Hsp92I | GRCGYC | 1 cut(s) | 507 |
| Hsp92II | CATG | 4 cut(s) | 931, 1537, 1676, 1760 |
| HspAI | GCGC | 2 cut(s) | 610, 1530 |
| Kpn2I | TCCGGA | 1 cut(s) | 1702 |
| Kzo9I | GATC | 3 cut(s) | 1637, 1668, 1699 |
| LmnI | GCTCC | 3 cut(s) | 31, 465, 1017 |
| Lsp1109I | GCAGC | 3 cut(s) | 1210, 1406, 1582 |
| LweI | GCATC | 3 cut(s) | 944, 1159, 1820 |
| MaeI | CTAG | 2 cut(s) | 1274, 1607 |
| MaeII | ACGT | 1 cut(s) | 599 |
| MaeIII | GTNAC | 2 cut(s) | 673, 1231 |
| MalI | GATC | 3 cut(s) | 1639, 1670, 1701 |
| MboI | GATC | 3 cut(s) | 1637, 1668, 1699 |
| MflI | RGATCY | 2 cut(s) | 1637, 1699 |
| MhlI | GDGCHC | 2 cut(s) | 225, 462 |
| MlsI | TGGCCA | 2 cut(s) | 5, 1056 |
| MluCI | AATT | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| MluNI | TGGCCA | 2 cut(s) | 5, 1056 |
| MlyI | GAGTC | 5 cut(s) | 69, 87, 348, 599, 958 |
| MmeI | TCCRAC | 1 cut(s) | 567 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 1056 |
| MroI | TCCGGA | 1 cut(s) | 1702 |
| MroXI | GAANNNNTTC | 1 cut(s) | 599 |
| MscI | TGGCCA | 2 cut(s) | 5, 1056 |
| MseI | TTAA | 4 cut(s) | 140, 1494, 1503, 1628 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1677 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 1056 |
| MspA1I | CMGCKG | 1 cut(s) | 562 |
| MspI | CCGG | 6 cut(s) | 75, 82, 379, 429, 715, 1703 |
| MspR9I | CCNGG | 6 cut(s) | 83, 380, 429, 635, 896, 1791 |
| MvaI | CCWGG | 3 cut(s) | 635, 896, 1791 |
| MvnI | CGCG | 6 cut(s) | 405, 423, 528, 610, 612, 832 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 381, 397, 417, 429, 838, 896, 1338, 1391, 1538 |
| NciI | CCSGG | 3 cut(s) | 83, 380, 429 |
| NdeII | GATC | 3 cut(s) | 1637, 1668, 1699 |
| NlaIII | CATG | 4 cut(s) | 931, 1537, 1676, 1760 |
| NlaIV | GGNNCC | 7 cut(s) | 27, 73, 224, 383, 632, 713, 933 |
| NmeAIII | GCCGAG | 1 cut(s) | 481 |
| NmuCI | GTSAC | 2 cut(s) | 673, 1231 |
| PaeR7I | CTCGAG | 1 cut(s) | 365 |
| PaqCI | CACCTGC | 1 cut(s) | 396 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 274, 453, 540, 552 |
| PdmI | GAANNNNTTC | 1 cut(s) | 599 |
| PfeI | GAWTC | 7 cut(s) | 53, 1120, 1144, 1219, 1432, 1683, 1731 |
| PflFI | GACNNNGTC | 1 cut(s) | 437 |
| PfoI | TCCNGGA | 1 cut(s) | 81 |
| PkrI | GCNGC | 8 cut(s) | 331, 374, 422, 527, 561, 1200, 1396, 1597 |
| PleI | GAGTC | 5 cut(s) | 68, 86, 347, 599, 958 |
| PpsI | GAGTC | 5 cut(s) | 68, 86, 347, 599, 958 |
| Psp124BI | GAGCTC | 1 cut(s) | 462 |
| Psp6I | CCWGG | 3 cut(s) | 633, 894, 1789 |
| PspFI | CCCAGC | 2 cut(s) | 787, 1214 |
| PspGI | CCWGG | 3 cut(s) | 633, 894, 1789 |
| PspN4I | GGNNCC | 7 cut(s) | 27, 73, 224, 383, 632, 713, 933 |
| PspPI | GGNCC | 3 cut(s) | 382, 932, 1376 |
| PstI | CTGCAG | 2 cut(s) | 1396, 1600 |
| PsuI | RGATCY | 2 cut(s) | 1637, 1699 |
| PsyI | GACNNNGTC | 1 cut(s) | 437 |
| RsaI | GTAC | 4 cut(s) | 42, 533, 578, 1286 |
| RsaNI | GTAC | 4 cut(s) | 41, 532, 577, 1285 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1677 |
| SacI | GAGCTC | 1 cut(s) | 462 |
| SaqAI | TTAA | 4 cut(s) | 140, 1494, 1503, 1628 |
| SatI | GCNGC | 8 cut(s) | 330, 373, 421, 526, 560, 1199, 1395, 1596 |
| Sau3AI | GATC | 3 cut(s) | 1637, 1668, 1699 |
| Sau96I | GGNCC | 3 cut(s) | 382, 932, 1376 |
| SchI | GAGTC | 5 cut(s) | 69, 87, 348, 599, 958 |
| ScrFI | CCNGG | 6 cut(s) | 83, 380, 429, 635, 896, 1791 |
| SduI | GDGCHC | 2 cut(s) | 225, 462 |
| SfaNI | GCATC | 3 cut(s) | 944, 1159, 1820 |
| SfcI | CTRYAG | 3 cut(s) | 1186, 1392, 1596 |
| Sfr274I | CTCGAG | 1 cut(s) | 365 |
| SinI | GGWCC | 1 cut(s) | 1376 |
| SlaI | CTCGAG | 1 cut(s) | 365 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1677 |
| SmlI | CTYRAG | 2 cut(s) | 10, 365 |
| SmoI | CTYRAG | 2 cut(s) | 10, 365 |
| Sse9I | AATT | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| SspMI | CTAG | 2 cut(s) | 1274, 1607 |
| SstI | GAGCTC | 1 cut(s) | 462 |
| StyD4I | CCNGG | 6 cut(s) | 81, 378, 427, 633, 894, 1789 |
| StyI | CCWWGG | 1 cut(s) | 1641 |
| TaaI | ACNGT | 6 cut(s) | 356, 855, 1051, 1153, 1282, 1740 |
| TaiI | ACGT | 1 cut(s) | 602 |
| TasI | AATT | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| TatI | WGTACW | 2 cut(s) | 576, 1284 |
| TauI | GCSGC | 5 cut(s) | 332, 375, 423, 528, 562 |
| TfiI | GAWTC | 7 cut(s) | 53, 1120, 1144, 1219, 1432, 1683, 1731 |
| Tru1I | TTAA | 4 cut(s) | 140, 1494, 1503, 1628 |
| Tru9I | TTAA | 4 cut(s) | 140, 1494, 1503, 1628 |
| TscAI | CASTG | 4 cut(s) | 132, 1287, 1743, 1857 |
| TseFI | GTSAC | 2 cut(s) | 673, 1231 |
| TseI | GCWGC | 3 cut(s) | 1198, 1394, 1595 |
| Tsp45I | GTSAC | 2 cut(s) | 673, 1231 |
| TspGWI | ACGGA | 2 cut(s) | 501, 692 |
| TspRI | CASTG | 4 cut(s) | 132, 1287, 1743, 1857 |
| Tth111I | GACNNNGTC | 1 cut(s) | 437 |
| VpaK11BI | GGWCC | 1 cut(s) | 1376 |
| XapI | RAATTY | 5 cut(s) | 622, 1291, 1579, 1623, 1652 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1567 |
| XhoI | CTCGAG | 1 cut(s) | 365 |
| XmnI | GAANNNNTTC | 1 cut(s) | 599 |
| XspI | CTAG | 2 cut(s) | 1274, 1607 |