Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 338 |
| AccB1I | GGYRCC | 1 cut(s) | 338 |
| AccBSI | CCGCTC | 2 cut(s) | 395, 1000 |
| AccI | GTMKAC | 2 cut(s) | 1563, 1779 |
| AccII | CGCG | 3 cut(s) | 757, 1007, 1486 |
| AccIII | TCCGGA | 1 cut(s) | 1849 |
| AciI | CCGC | 6 cut(s) | 393, 526, 670, 840, 979, 1000 |
| AclWI | GGATC | 4 cut(s) | 244, 1779, 1810, 1841 |
| AcsI | RAATTY | 3 cut(s) | 439, 1438, 1726 |
| AcuI | CTGAAG | 2 cut(s) | 445, 1167 |
| AcvI | CACGTG | 1 cut(s) | 48 |
| AfaI | GTAC | 3 cut(s) | 340, 680, 1923 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 334, 377, 532, 1087 |
| AgsI | TTSAA | 9 cut(s) | 667, 925, 1193, 1394, 1474, 1579, 1595, 1631, 2005 |
| AjnI | CCWGG | 2 cut(s) | 1442, 1936 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 351, 383, 917, 949, 1130, 1162 |
| AluBI | AGCT | 9 cut(s) | 160, 459, 604, 613, 706, 736, 1093, 1252, 1479 |
| AluI | AGCT | 9 cut(s) | 160, 459, 604, 613, 706, 736, 1093, 1252, 1479 |
| Alw21I | GWGCWC | 2 cut(s) | 606, 1236 |
| Alw26I | GTCTC | 2 cut(s) | 183, 579 |
| AlwI | GGATC | 4 cut(s) | 244, 1779, 1810, 1841 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 138 |
| Aor13HI | TCCGGA | 1 cut(s) | 1849 |
| AoxI | GGCC | 4 cut(s) | 89, 535, 551, 797 |
| ApeKI | GCWGC | 4 cut(s) | 482, 1402, 1541, 1742 |
| ApoI | RAATTY | 3 cut(s) | 439, 1438, 1726 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 736, 768 |
| Asp718I | GGTACC | 1 cut(s) | 338 |
| AspLEI | GCGC | 4 cut(s) | 487, 1007, 1407, 1488 |
| AspS9I | GGNCC | 5 cut(s) | 89, 320, 493, 535, 1523 |
| AsuC2I | CCSGG | 2 cut(s) | 101, 533 |
| AsuHPI | GGTGA | 8 cut(s) | 163, 262, 376, 389, 707, 713, 854, 881 |
| AvaII | GGWCC | 3 cut(s) | 320, 493, 1523 |
| AxyI | CCTNAGG | 1 cut(s) | 1032 |
| BaeGI | GKGCMC | 1 cut(s) | 1082 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 322, 355 |
| BanI | GGYRCC | 1 cut(s) | 338 |
| BanII | GRGCYC | 1 cut(s) | 606 |
| BbrPI | CACGTG | 1 cut(s) | 48 |
| BbsI | GAAGAC | 1 cut(s) | 924 |
| Bbv12I | GWGCWC | 2 cut(s) | 606, 1236 |
| BbvCI | CCTCAGC | 1 cut(s) | 125 |
| BbvI | GCAGC | 4 cut(s) | 469, 1389, 1553, 1729 |
| BccI | CCATC | 4 cut(s) | 128, 380, 692, 1652 |
| BciT130I | CCWGG | 2 cut(s) | 1444, 1938 |
| BciVI | GTATCC | 1 cut(s) | 755 |
| BcnI | CCSGG | 2 cut(s) | 101, 533 |
| BcoDI | GTCTC | 2 cut(s) | 183, 579 |
| BfaI | CTAG | 4 cut(s) | 518, 884, 1421, 1754 |
| BfmI | CTRYAG | 5 cut(s) | 410, 1333, 1383, 1539, 1743 |
| BfuI | GTATCC | 1 cut(s) | 755 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 534 |
| BisI | GCNGC | 5 cut(s) | 483, 526, 1403, 1542, 1743 |
| BlpI | GCTNAGC | 1 cut(s) | 609 |
| BlsI | GCNGC | 5 cut(s) | 484, 527, 1404, 1543, 1744 |
| Bme1390I | CCNGG | 4 cut(s) | 101, 533, 1444, 1938 |
| Bme18I | GGWCC | 3 cut(s) | 320, 493, 1523 |
| BmgT120I | GGNCC | 5 cut(s) | 89, 320, 493, 535, 1523 |
| BmiI | GGNNCC | 7 cut(s) | 90, 322, 340, 494, 495, 536, 779 |
| BmrFI | CCNGG | 4 cut(s) | 101, 533, 1444, 1938 |
| BmrI | ACTGGG | 1 cut(s) | 1074 |
| BmsI | GCATC | 3 cut(s) | 70, 1306, 1747 |
| BmuI | ACTGGG | 1 cut(s) | 1074 |
| BoxI | GACNNNNGTC | 2 cut(s) | 175, 1301 |
| BpiI | GAAGAC | 1 cut(s) | 924 |
| Bpu10I | CCTNAGC | 1 cut(s) | 125 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 609 |
| BpuEI | CTTGAG | 2 cut(s) | 1370, 1827 |
| BpuMI | CCSGG | 2 cut(s) | 101, 533 |
| BsaAI | YACGTR | 2 cut(s) | 48, 766 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1578 |
| BsaJI | CCNNGG | 5 cut(s) | 39, 532, 690, 1788, 1836 |
| BsaWI | WCCGGW | 1 cut(s) | 1849 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 312, 342, 576, 606 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 334, 377, 532, 1087 |
| Bse1I | ACTGG | 3 cut(s) | 554, 1069, 1133 |
| Bse21I | CCTNAGG | 1 cut(s) | 1032 |
| Bse3DI | GCAATG | 3 cut(s) | 477, 1495, 1539 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1578 |
| BseAI | TCCGGA | 1 cut(s) | 1849 |
| BseBI | CCWGG | 2 cut(s) | 1444, 1938 |
| BseDI | CCNNGG | 5 cut(s) | 39, 532, 690, 1788, 1836 |
| BseGI | GGATG | 9 cut(s) | 120, 681, 829, 901, 946, 1663, 1798, 1953, 1965 |
| BseJI | GATNNNNATC | 1 cut(s) | 1578 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 334, 377, 532, 1087 |
| BseMI | GCAATG | 3 cut(s) | 477, 1495, 1539 |
| BseMII | CTCAG | 7 cut(s) | 139, 181, 188, 600, 891, 1023, 1214 |
| BseNI | ACTGG | 3 cut(s) | 554, 1069, 1133 |
| BseRI | GAGGAG | 2 cut(s) | 176, 832 |
| BseSI | GKGCMC | 1 cut(s) | 1082 |
| BseXI | GCAGC | 4 cut(s) | 469, 1389, 1553, 1729 |
| Bsh1236I | CGCG | 3 cut(s) | 757, 1007, 1486 |
| BshFI | GGCC | 4 cut(s) | 91, 537, 553, 799 |
| BshNI | GGYRCC | 1 cut(s) | 338 |
| BsiHKAI | GWGCWC | 2 cut(s) | 606, 1236 |
| BsiSI | CCGG | 5 cut(s) | 101, 532, 582, 673, 1850 |
| BslFI | GGGAC | 1 cut(s) | 479 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 334, 377, 532, 1087 |
| BsmAI | GTCTC | 2 cut(s) | 183, 579 |
| BsmBI | CGTCTC | 1 cut(s) | 579 |
| BsmFI | GGGAC | 1 cut(s) | 479 |
| BsmI | GAATGC | 1 cut(s) | 558 |
| BsnI | GGCC | 4 cut(s) | 91, 537, 553, 799 |
| Bsp1286I | GDGCHC | 3 cut(s) | 606, 1082, 1236 |
| Bsp13I | TCCGGA | 1 cut(s) | 1849 |
| Bsp143I | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 609 |
| Bsp19I | CCATGG | 1 cut(s) | 1788 |
| BspACI | CCGC | 6 cut(s) | 393, 526, 670, 840, 979, 1000 |
| BspANI | GGCC | 4 cut(s) | 91, 537, 553, 799 |
| BspCNI | CTCAG | 7 cut(s) | 138, 180, 187, 601, 890, 1024, 1215 |
| BspEI | TCCGGA | 1 cut(s) | 1849 |
| BspFNI | CGCG | 3 cut(s) | 757, 1007, 1486 |
| BspLI | GGNNCC | 7 cut(s) | 90, 322, 340, 494, 495, 536, 779 |
| BspMAI | CTGCAG | 2 cut(s) | 1543, 1747 |
| BspPI | GGATC | 4 cut(s) | 244, 1779, 1810, 1841 |
| BspQI | GCTCTTC | 1 cut(s) | 594 |
| BspT107I | GGYRCC | 1 cut(s) | 338 |
| BsrBI | CCGCTC | 2 cut(s) | 395, 1000 |
| BsrDI | GCAATG | 3 cut(s) | 477, 1495, 1539 |
| BsrI | ACTGG | 3 cut(s) | 554, 1069, 1133 |
| BssECI | CCNNGG | 5 cut(s) | 39, 532, 690, 1788, 1836 |
| BssMI | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| BssNAI | GTATAC | 1 cut(s) | 1564 |
| BssT1I | CCWWGG | 3 cut(s) | 39, 1788, 1836 |
| Bst1107I | GTATAC | 1 cut(s) | 1564 |
| Bst2UI | CCWGG | 2 cut(s) | 1444, 1938 |
| Bst4CI | ACNGT | 5 cut(s) | 401, 509, 1198, 1300, 1387 |
| Bst6I | CTCTTC | 6 cut(s) | 186, 314, 429, 594, 809, 848 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1538 |
| BstBAI | YACGTR | 2 cut(s) | 48, 766 |
| BstC8I | GCNNGC | 3 cut(s) | 606, 738, 1449 |
| BstDEI | CTNAG | 8 cut(s) | 125, 167, 174, 609, 877, 1032, 1223, 1974 |
| BstDSI | CCRYGG | 1 cut(s) | 1788 |
| BstEII | GGTNACC | 1 cut(s) | 151 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 332 |
| BstF5I | GGATG | 9 cut(s) | 120, 681, 829, 901, 946, 1663, 1798, 1953, 1965 |
| BstFNI | CGCG | 3 cut(s) | 757, 1007, 1486 |
| BstHHI | GCGC | 4 cut(s) | 487, 1007, 1407, 1488 |
| BstKTI | GATC | 5 cut(s) | 252, 1420, 1787, 1818, 1849 |
| BstMAI | GTCTC | 2 cut(s) | 183, 579 |
| BstMBI | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 534, 550, 578, 610, 1485, 1538, 1685 |
| BstNI | CCWGG | 2 cut(s) | 1444, 1938 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 175, 1301 |
| BstPI | GGTNACC | 1 cut(s) | 151 |
| BstSCI | CCNGG | 4 cut(s) | 99, 531, 1442, 1936 |
| BstSFI | CTRYAG | 5 cut(s) | 410, 1333, 1383, 1539, 1743 |
| BstSLI | GKGCMC | 1 cut(s) | 1082 |
| BstUI | CGCG | 3 cut(s) | 757, 1007, 1486 |
| BstV1I | GCAGC | 4 cut(s) | 469, 1389, 1553, 1729 |
| BstV2I | GAAGAC | 1 cut(s) | 924 |
| BstX2I | RGATCY | 2 cut(s) | 1784, 1846 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1826, 1944 |
| BstYI | RGATCY | 2 cut(s) | 1784, 1846 |
| BstZ17I | GTATAC | 1 cut(s) | 1564 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1032 |
| BsuI | GTATCC | 1 cut(s) | 755 |
| BsuRI | GGCC | 4 cut(s) | 91, 537, 553, 799 |
| BtgI | CCRYGG | 1 cut(s) | 1788 |
| BtgZI | GCGATG | 1 cut(s) | 553 |
| BtsCI | GGATG | 9 cut(s) | 120, 681, 829, 901, 946, 1663, 1798, 1953, 1965 |
| BtsIMutI | CAGTG | 2 cut(s) | 885, 1062 |
| Cac8I | GCNNGC | 3 cut(s) | 606, 738, 1449 |
| CaiI | CAGNNNCTG | 1 cut(s) | 138 |
| CfoI | GCGC | 4 cut(s) | 487, 1007, 1407, 1488 |
| Cfr13I | GGNCC | 5 cut(s) | 89, 320, 493, 535, 1523 |
| CseI | GACGC | 1 cut(s) | 1748 |
| Csp6I | GTAC | 3 cut(s) | 339, 679, 1922 |
| CviAII | CATG | 5 cut(s) | 145, 1654, 1715, 1789, 1820 |
| CviQI | GTAC | 3 cut(s) | 339, 679, 1922 |
| DdeI | CTNAG | 8 cut(s) | 125, 167, 174, 609, 877, 1032, 1223, 1974 |
| DpnI | GATC | 5 cut(s) | 251, 1419, 1786, 1817, 1848 |
| DpnII | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| Eam1104I | CTCTTC | 6 cut(s) | 186, 314, 429, 594, 809, 848 |
| EarI | CTCTTC | 6 cut(s) | 186, 314, 429, 594, 809, 848 |
| Ecl136II | GAGCTC | 1 cut(s) | 604 |
| Eco130I | CCWWGG | 3 cut(s) | 39, 1788, 1836 |
| Eco24I | GRGCYC | 1 cut(s) | 606 |
| Eco47I | GGWCC | 3 cut(s) | 320, 493, 1523 |
| Eco53kI | GAGCTC | 1 cut(s) | 604 |
| Eco57I | CTGAAG | 2 cut(s) | 445, 1167 |
| Eco72I | CACGTG | 1 cut(s) | 48 |
| Eco81I | CCTNAGG | 1 cut(s) | 1032 |
| Eco91I | GGTNACC | 1 cut(s) | 151 |
| EcoICRI | GAGCTC | 1 cut(s) | 604 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 332 |
| EcoO109I | RGGNCCY | 2 cut(s) | 89, 493 |
| EcoO65I | GGTNACC | 1 cut(s) | 151 |
| EcoRI | GAATTC | 1 cut(s) | 1438 |
| EcoRII | CCWGG | 2 cut(s) | 1442, 1936 |
| EcoT14I | CCWWGG | 3 cut(s) | 39, 1788, 1836 |
| EcoT22I | ATGCAT | 1 cut(s) | 1762 |
| EcoT38I | GRGCYC | 1 cut(s) | 606 |
| ErhI | CCWWGG | 3 cut(s) | 39, 1788, 1836 |
| Esp3I | CGTCTC | 1 cut(s) | 579 |
| FaeI | CATG | 5 cut(s) | 148, 1657, 1718, 1792, 1823 |
| FaqI | GGGAC | 1 cut(s) | 479 |
| FatI | CATG | 5 cut(s) | 144, 1653, 1714, 1788, 1819 |
| FauI | CCCGC | 2 cut(s) | 400, 986 |
| FblI | GTMKAC | 2 cut(s) | 1563, 1779 |
| Fnu4HI | GCNGC | 5 cut(s) | 483, 526, 1403, 1542, 1743 |
| FokI | GGATG | 9 cut(s) | 107, 688, 836, 908, 953, 1670, 1805, 1952, 1960 |
| FriOI | GRGCYC | 1 cut(s) | 606 |
| Fsp4HI | GCNGC | 5 cut(s) | 483, 526, 1403, 1542, 1743 |
| FspBI | CTAG | 4 cut(s) | 518, 884, 1421, 1754 |
| GlaI | GCGC | 4 cut(s) | 486, 1006, 1406, 1487 |
| GluI | GCNGC | 5 cut(s) | 483, 526, 1403, 1542, 1743 |
| HaeIII | GGCC | 4 cut(s) | 91, 537, 553, 799 |
| HapII | CCGG | 5 cut(s) | 101, 532, 582, 673, 1850 |
| HgaI | GACGC | 1 cut(s) | 1748 |
| HhaI | GCGC | 4 cut(s) | 487, 1007, 1407, 1488 |
| Hin1II | CATG | 5 cut(s) | 148, 1657, 1718, 1792, 1823 |
| Hin6I | GCGC | 4 cut(s) | 485, 1005, 1405, 1486 |
| HinP1I | GCGC | 4 cut(s) | 485, 1005, 1405, 1486 |
| HincII | GTYRAC | 1 cut(s) | 404 |
| HindII | GTYRAC | 1 cut(s) | 404 |
| HindIII | AAGCTT | 3 cut(s) | 457, 734, 1250 |
| HinfI | GANTC | 8 cut(s) | 381, 576, 622, 752, 1291, 1366, 1579, 1830 |
| HpaII | CCGG | 5 cut(s) | 101, 532, 582, 673, 1850 |
| HphI | GGTGA | 8 cut(s) | 163, 262, 376, 389, 707, 713, 854, 881 |
| Hpy166II | GTNNAC | 3 cut(s) | 404, 1564, 1780 |
| Hpy188I | TCNGA | 8 cut(s) | 76, 170, 298, 691, 777, 1296, 1869, 1891 |
| Hpy188III | TCNNGA | 6 cut(s) | 1163, 1271, 1601, 1754, 1844, 1850 |
| Hpy8I | GTNNAC | 3 cut(s) | 404, 1564, 1780 |
| HpyAV | CCTTC | 9 cut(s) | 224, 830, 839, 859, 905, 919, 1160, 1191, 1656 |
| HpyCH4III | ACNGT | 5 cut(s) | 401, 509, 1198, 1300, 1387 |
| HpyCH4IV | ACGT | 2 cut(s) | 47, 765 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 534, 550, 578, 610, 1485, 1538, 1685 |
| HpyF3I | CTNAG | 8 cut(s) | 125, 167, 174, 609, 877, 1032, 1223, 1974 |
| HpySE526I | ACGT | 2 cut(s) | 47, 765 |
| Hsp92II | CATG | 5 cut(s) | 148, 1657, 1718, 1792, 1823 |
| HspAI | GCGC | 4 cut(s) | 485, 1005, 1405, 1486 |
| KflI | GGGWCCC | 1 cut(s) | 493 |
| Kpn2I | TCCGGA | 1 cut(s) | 1849 |
| KpnI | GGTACC | 1 cut(s) | 342 |
| Kzo9I | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| LguI | GCTCTTC | 1 cut(s) | 594 |
| LmnI | GCTCC | 2 cut(s) | 237, 1090 |
| Lsp1109I | GCAGC | 4 cut(s) | 469, 1389, 1553, 1729 |
| LweI | GCATC | 3 cut(s) | 70, 1306, 1747 |
| MaeI | CTAG | 4 cut(s) | 518, 884, 1421, 1754 |
| MaeII | ACGT | 2 cut(s) | 47, 765 |
| MaeIII | GTNAC | 4 cut(s) | 43, 140, 151, 1378 |
| MalI | GATC | 5 cut(s) | 251, 1419, 1786, 1817, 1848 |
| MbiI | CCGCTC | 2 cut(s) | 395, 1000 |
| MboI | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| MfeI | CAATTG | 1 cut(s) | 1590 |
| MflI | RGATCY | 2 cut(s) | 1784, 1846 |
| MhlI | GDGCHC | 3 cut(s) | 606, 1082, 1236 |
| MluCI | AATT | 9 cut(s) | 242, 351, 439, 1311, 1438, 1590, 1638, 1726, 1878 |
| MlyI | GAGTC | 1 cut(s) | 585 |
| MmeI | TCCRAC | 1 cut(s) | 99 |
| Mph1103I | ATGCAT | 1 cut(s) | 1762 |
| MroI | TCCGGA | 1 cut(s) | 1849 |
| MseI | TTAA | 6 cut(s) | 66, 245, 443, 710, 1641, 1650 |
| MslI | CAYNNNNRTG | 2 cut(s) | 149, 1824 |
| MspI | CCGG | 5 cut(s) | 101, 532, 582, 673, 1850 |
| MspR9I | CCNGG | 4 cut(s) | 101, 533, 1444, 1938 |
| MunI | CAATTG | 1 cut(s) | 1590 |
| Mva1269I | GAATGC | 1 cut(s) | 558 |
| MvaI | CCWGG | 2 cut(s) | 1444, 1938 |
| MvnI | CGCG | 3 cut(s) | 757, 1007, 1486 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 534, 550, 578, 610, 1485, 1538, 1685 |
| NciI | CCSGG | 2 cut(s) | 101, 533 |
| NcoI | CCATGG | 1 cut(s) | 1788 |
| NdeII | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| NlaIII | CATG | 5 cut(s) | 148, 1657, 1718, 1792, 1823 |
| NlaIV | GGNNCC | 7 cut(s) | 90, 322, 340, 494, 495, 536, 779 |
| NmuCI | GTSAC | 3 cut(s) | 43, 151, 1378 |
| NsiI | ATGCAT | 1 cut(s) | 1762 |
| PciSI | GCTCTTC | 1 cut(s) | 594 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 585, 687 |
| PctI | GAATGC | 1 cut(s) | 558 |
| PfeI | GAWTC | 7 cut(s) | 381, 622, 752, 1291, 1366, 1579, 1830 |
| PkrI | GCNGC | 5 cut(s) | 484, 527, 1404, 1543, 1744 |
| PleI | GAGTC | 1 cut(s) | 584 |
| PmaCI | CACGTG | 1 cut(s) | 48 |
| PmlI | CACGTG | 1 cut(s) | 48 |
| PpsI | GAGTC | 1 cut(s) | 584 |
| Ppu21I | YACGTR | 2 cut(s) | 48, 766 |
| PpuMI | RGGWCCY | 1 cut(s) | 493 |
| PshAI | GACNNNNGTC | 2 cut(s) | 175, 1301 |
| Psp124BI | GAGCTC | 1 cut(s) | 606 |
| Psp5II | RGGWCCY | 1 cut(s) | 493 |
| Psp6I | CCWGG | 2 cut(s) | 1442, 1936 |
| PspCI | CACGTG | 1 cut(s) | 48 |
| PspEI | GGTNACC | 1 cut(s) | 151 |
| PspGI | CCWGG | 2 cut(s) | 1442, 1936 |
| PspN4I | GGNNCC | 7 cut(s) | 90, 322, 340, 494, 495, 536, 779 |
| PspPI | GGNCC | 5 cut(s) | 89, 320, 493, 535, 1523 |
| PspPPI | RGGWCCY | 1 cut(s) | 493 |
| PstI | CTGCAG | 2 cut(s) | 1543, 1747 |
| PstNI | CAGNNNCTG | 1 cut(s) | 138 |
| PsuI | RGATCY | 2 cut(s) | 1784, 1846 |
| RsaI | GTAC | 3 cut(s) | 340, 680, 1923 |
| RsaNI | GTAC | 3 cut(s) | 339, 679, 1922 |
| RseI | CAYNNNNRTG | 2 cut(s) | 149, 1824 |
| SacI | GAGCTC | 1 cut(s) | 606 |
| SapI | GCTCTTC | 1 cut(s) | 594 |
| SaqAI | TTAA | 6 cut(s) | 66, 245, 443, 710, 1641, 1650 |
| SatI | GCNGC | 5 cut(s) | 483, 526, 1403, 1542, 1743 |
| Sau3AI | GATC | 5 cut(s) | 249, 1417, 1784, 1815, 1846 |
| Sau96I | GGNCC | 5 cut(s) | 89, 320, 493, 535, 1523 |
| SchI | GAGTC | 1 cut(s) | 585 |
| ScrFI | CCNGG | 4 cut(s) | 101, 533, 1444, 1938 |
| SduI | GDGCHC | 3 cut(s) | 606, 1082, 1236 |
| SfaNI | GCATC | 3 cut(s) | 70, 1306, 1747 |
| SfcI | CTRYAG | 5 cut(s) | 410, 1333, 1383, 1539, 1743 |
| SinI | GGWCC | 3 cut(s) | 320, 493, 1523 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 149, 1824 |
| SmlI | CTYRAG | 2 cut(s) | 1349, 1842 |
| SmoI | CTYRAG | 2 cut(s) | 1349, 1842 |
| Sse9I | AATT | 9 cut(s) | 242, 351, 439, 1311, 1438, 1590, 1638, 1726, 1878 |
| SsiI | CCGC | 6 cut(s) | 393, 526, 670, 840, 979, 1000 |
| SspI | AATATT | 2 cut(s) | 25, 1773 |
| SspMI | CTAG | 4 cut(s) | 518, 884, 1421, 1754 |
| SstI | GAGCTC | 1 cut(s) | 606 |
| StyD4I | CCNGG | 4 cut(s) | 99, 531, 1442, 1936 |
| StyI | CCWWGG | 3 cut(s) | 39, 1788, 1836 |
| TaaI | ACNGT | 5 cut(s) | 401, 509, 1198, 1300, 1387 |
| TaiI | ACGT | 2 cut(s) | 50, 768 |
| TaqI | TCGA | 4 cut(s) | 750, 794, 974, 1765 |
| TasI | AATT | 9 cut(s) | 242, 351, 439, 1311, 1438, 1590, 1638, 1726, 1878 |
| TauI | GCSGC | 1 cut(s) | 528 |
| TfiI | GAWTC | 7 cut(s) | 381, 622, 752, 1291, 1366, 1579, 1830 |
| Tru1I | TTAA | 6 cut(s) | 66, 245, 443, 710, 1641, 1650 |
| Tru9I | TTAA | 6 cut(s) | 66, 245, 443, 710, 1641, 1650 |
| TscAI | CASTG | 2 cut(s) | 885, 1069 |
| TseFI | GTSAC | 3 cut(s) | 43, 151, 1378 |
| TseI | GCWGC | 4 cut(s) | 482, 1402, 1541, 1742 |
| Tsp45I | GTSAC | 3 cut(s) | 43, 151, 1378 |
| TspGWI | ACGGA | 1 cut(s) | 696 |
| TspRI | CASTG | 2 cut(s) | 885, 1069 |
| VpaK11BI | GGWCC | 3 cut(s) | 320, 493, 1523 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 332 |
| XapI | RAATTY | 3 cut(s) | 439, 1438, 1726 |
| XbaI | TCTAGA | 1 cut(s) | 1753 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1714 |
| XmiI | GTMKAC | 2 cut(s) | 1563, 1779 |
| XspI | CTAG | 4 cut(s) | 518, 884, 1421, 1754 |
| Zsp2I | ATGCAT | 1 cut(s) | 1762 |