Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT4G27340 AT4G27340 AT4G27340 |
| fragaria_vesca | FvH4_3g21210 |
| malus_domestica | MD03G1217000.v1.1 |
| prunus_persica | Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 |
| pyrus_communis | pycom03g16620 |
| rosa_chinensis | RchiOBHm_Chr5g0036281 |
| rosa_laevigata | RLG00000033683 |
| rosa_multiflora | Rmu_sc0000247.1_g000072 |
| rosa_roxburghii | Rroxscaffold_1G00044510 |
| rosa_rugosa | Rorug05G0154500 |
| rosa_samantha | Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500 |
| rosa_wichuraiana | Rw5G022770 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 378 |
| Acc36I | ACCTGC | 1 cut(s) | 378 |
| AccB1I | GGYRCC | 1 cut(s) | 203 |
| AccII | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| AccIII | TCCGGA | 1 cut(s) | 1690 |
| AclWI | GGATC | 3 cut(s) | 1620, 1651, 1682 |
| AcoI | YGGCCR | 2 cut(s) | 400, 1042 |
| AcsI | RAATTY | 6 cut(s) | 62, 607, 1279, 1567, 1611, 1640 |
| AcuI | CTGAAG | 2 cut(s) | 274, 1704 |
| AcyI | GRCGYC | 1 cut(s) | 492 |
| AfaI | GTAC | 3 cut(s) | 518, 563, 1274 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 199, 361 |
| AgsI | TTSAA | 9 cut(s) | 304, 766, 1034, 1166, 1193, 1315, 1420, 1436, 1833 |
| AjnI | CCWGG | 3 cut(s) | 618, 882, 1777 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 208, 240, 971, 1003 |
| Alw21I | GWGCWC | 1 cut(s) | 444 |
| Alw26I | GTCTC | 2 cut(s) | 127, 468 |
| AlwI | GGATC | 3 cut(s) | 1620, 1651, 1682 |
| Ama87I | CYCGRG | 1 cut(s) | 347 |
| Aor13HI | TCCGGA | 1 cut(s) | 1690 |
| AoxI | GGCC | 7 cut(s) | 364, 380, 400, 635, 920, 1026, 1042 |
| ApeKI | GCWGC | 3 cut(s) | 1186, 1382, 1583 |
| ApoI | RAATTY | 6 cut(s) | 62, 607, 1279, 1567, 1611, 1640 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 584 |
| AspLEI | GCGC | 2 cut(s) | 597, 1520 |
| AspS9I | GGNCC | 4 cut(s) | 185, 364, 920, 1364 |
| AsuC2I | CCSGG | 2 cut(s) | 362, 411 |
| AsuHPI | GGTGA | 5 cut(s) | 670, 692, 719, 1117, 1573 |
| AvaI | CYCGRG | 1 cut(s) | 347 |
| AvaII | GGWCC | 2 cut(s) | 185, 1364 |
| AxyI | CCTNAGG | 1 cut(s) | 873 |
| BaeGI | GKGCMC | 1 cut(s) | 206 |
| BanI | GGYRCC | 1 cut(s) | 203 |
| BanII | GRGCYC | 1 cut(s) | 444 |
| BbsI | GAAGAC | 3 cut(s) | 432, 486, 587 |
| Bbv12I | GWGCWC | 1 cut(s) | 444 |
| BbvI | GCAGC | 3 cut(s) | 1198, 1394, 1570 |
| BccI | CCATC | 4 cut(s) | 113, 798, 1493, 1713 |
| BceAI | ACGGC | 4 cut(s) | 505, 547, 553, 691 |
| BciT130I | CCWGG | 3 cut(s) | 620, 884, 1779 |
| BcnI | CCSGG | 2 cut(s) | 362, 411 |
| BcoDI | GTCTC | 2 cut(s) | 127, 468 |
| BfaI | CTAG | 2 cut(s) | 1262, 1595 |
| BfmI | CTRYAG | 3 cut(s) | 1174, 1380, 1584 |
| BfoI | RGCGCY | 1 cut(s) | 1521 |
| BfuAI | ACCTGC | 1 cut(s) | 378 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 363 |
| BisI | GCNGC | 7 cut(s) | 312, 355, 511, 545, 1187, 1383, 1584 |
| BlsI | GCNGC | 7 cut(s) | 313, 356, 512, 546, 1188, 1384, 1585 |
| Bme1390I | CCNGG | 5 cut(s) | 362, 411, 620, 884, 1779 |
| Bme18I | GGWCC | 2 cut(s) | 185, 1364 |
| BmeT110I | CYCGRG | 1 cut(s) | 347 |
| BmgT120I | GGNCC | 4 cut(s) | 185, 364, 920, 1364 |
| BmiI | GGNNCC | 6 cut(s) | 187, 205, 365, 617, 698, 921 |
| BmrFI | CCNGG | 5 cut(s) | 362, 411, 620, 884, 1779 |
| BmsI | GCATC | 4 cut(s) | 932, 1147, 1588, 1808 |
| BpiI | GAAGAC | 3 cut(s) | 432, 486, 587 |
| BpmI | CTGGAG | 2 cut(s) | 743, 1377 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1101 |
| BpuMI | CCSGG | 2 cut(s) | 362, 411 |
| Bsa29I | ATCGAT | 1 cut(s) | 1155 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1419 |
| BsaHI | GRCGYC | 1 cut(s) | 492 |
| BsaJI | CCNNGG | 4 cut(s) | 361, 409, 1629, 1778 |
| BsaWI | WCCGGW | 2 cut(s) | 699, 1690 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 72, 102, 177, 207 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 199, 361 |
| Bse1I | ACTGG | 4 cut(s) | 383, 974, 1280, 1838 |
| Bse21I | CCTNAGG | 1 cut(s) | 873 |
| Bse3DI | GCAATG | 1 cut(s) | 1336 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1419 |
| BseAI | TCCGGA | 1 cut(s) | 1690 |
| BseBI | CCWGG | 3 cut(s) | 620, 884, 1779 |
| BseCI | ATCGAT | 1 cut(s) | 1155 |
| BseDI | CCNNGG | 4 cut(s) | 361, 409, 1629, 1778 |
| BseGI | GGATG | 8 cut(s) | 105, 742, 787, 841, 1504, 1639, 1794, 1849 |
| BseJI | GATNNNNATC | 1 cut(s) | 1419 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 199, 361 |
| BseMI | GCAATG | 1 cut(s) | 1336 |
| BseMII | CTCAG | 3 cut(s) | 37, 887, 1055 |
| BseNI | ACTGG | 4 cut(s) | 383, 974, 1280, 1838 |
| BseRI | GAGGAG | 5 cut(s) | 11, 275, 434, 667, 878 |
| BseSI | GKGCMC | 1 cut(s) | 206 |
| BseX3I | CGGCCG | 1 cut(s) | 400 |
| BseXI | GCAGC | 3 cut(s) | 1198, 1394, 1570 |
| BseYI | CCCAGC | 2 cut(s) | 775, 1202 |
| Bsh1236I | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| Bsh1285I | CGRYCG | 1 cut(s) | 403 |
| BshFI | GGCC | 7 cut(s) | 366, 382, 402, 637, 922, 1028, 1044 |
| BshNI | GGYRCC | 1 cut(s) | 203 |
| BshVI | ATCGAT | 1 cut(s) | 1155 |
| BsiEI | CGRYCG | 1 cut(s) | 403 |
| BsiHKAI | GWGCWC | 1 cut(s) | 444 |
| BsiHKCI | CYCGRG | 1 cut(s) | 347 |
| BsiSI | CCGG | 4 cut(s) | 361, 411, 700, 1691 |
| BslFI | GGGAC | 2 cut(s) | 308, 392 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 199, 361 |
| BsmAI | GTCTC | 2 cut(s) | 127, 468 |
| BsmBI | CGTCTC | 2 cut(s) | 127, 468 |
| BsmFI | GGGAC | 2 cut(s) | 308, 392 |
| BsnI | GGCC | 7 cut(s) | 366, 382, 402, 637, 922, 1028, 1044 |
| BsoBI | CYCGRG | 1 cut(s) | 347 |
| Bsp1286I | GDGCHC | 2 cut(s) | 206, 444 |
| Bsp13I | TCCGGA | 1 cut(s) | 1690 |
| Bsp1407I | TGTACA | 1 cut(s) | 561 |
| Bsp143I | GATC | 3 cut(s) | 1625, 1656, 1687 |
| BspANI | GGCC | 7 cut(s) | 366, 382, 402, 637, 922, 1028, 1044 |
| BspCNI | CTCAG | 3 cut(s) | 36, 886, 1056 |
| BspDI | ATCGAT | 1 cut(s) | 1155 |
| BspEI | TCCGGA | 1 cut(s) | 1690 |
| BspFNI | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| BspLI | GGNNCC | 6 cut(s) | 187, 205, 365, 617, 698, 921 |
| BspMAI | CTGCAG | 2 cut(s) | 1384, 1588 |
| BspMI | ACCTGC | 1 cut(s) | 378 |
| BspPI | GGATC | 3 cut(s) | 1620, 1651, 1682 |
| BspQI | GCTCTTC | 1 cut(s) | 20 |
| BspT107I | GGYRCC | 1 cut(s) | 203 |
| BsrDI | GCAATG | 1 cut(s) | 1336 |
| BsrGI | TGTACA | 1 cut(s) | 561 |
| BsrI | ACTGG | 4 cut(s) | 383, 974, 1280, 1838 |
| BssECI | CCNNGG | 4 cut(s) | 361, 409, 1629, 1778 |
| BssMI | GATC | 3 cut(s) | 1625, 1656, 1687 |
| BssNI | GRCGYC | 1 cut(s) | 492 |
| BssT1I | CCWWGG | 1 cut(s) | 1629 |
| Bst2UI | CCWGG | 3 cut(s) | 620, 884, 1779 |
| Bst4CI | ACNGT | 6 cut(s) | 338, 843, 1039, 1141, 1270, 1728 |
| Bst6I | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 644, 686, 794 |
| BstACI | GRCGYC | 1 cut(s) | 492 |
| BstAUI | TGTACA | 1 cut(s) | 561 |
| BstC8I | GCNNGC | 4 cut(s) | 542, 576, 924, 1649 |
| BstDEI | CTNAG | 4 cut(s) | 23, 873, 1064, 1101 |
| BstEII | GGTNACC | 1 cut(s) | 4 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 197 |
| BstF5I | GGATG | 8 cut(s) | 105, 742, 787, 841, 1504, 1639, 1794, 1849 |
| BstFNI | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| BstH2I | RGCGCY | 1 cut(s) | 1521 |
| BstHHI | GCGC | 2 cut(s) | 597, 1520 |
| BstKTI | GATC | 3 cut(s) | 1628, 1659, 1690 |
| BstMAI | GTCTC | 2 cut(s) | 127, 468 |
| BstMBI | GATC | 3 cut(s) | 1625, 1656, 1687 |
| BstMCI | CGRYCG | 1 cut(s) | 403 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 363, 379, 399, 826, 884, 1326, 1379, 1526 |
| BstNI | CCWGG | 3 cut(s) | 620, 884, 1779 |
| BstPI | GGTNACC | 1 cut(s) | 4 |
| BstSCI | CCNGG | 5 cut(s) | 360, 409, 618, 882, 1777 |
| BstSFI | CTRYAG | 3 cut(s) | 1174, 1380, 1584 |
| BstSLI | GKGCMC | 1 cut(s) | 206 |
| BstUI | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| BstV1I | GCAGC | 3 cut(s) | 1198, 1394, 1570 |
| BstV2I | GAAGAC | 3 cut(s) | 432, 486, 587 |
| BstX2I | RGATCY | 2 cut(s) | 1625, 1687 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1667, 1785 |
| BstYI | RGATCY | 2 cut(s) | 1625, 1687 |
| BstZI | CGGCCG | 1 cut(s) | 400 |
| Bsu15I | ATCGAT | 1 cut(s) | 1155 |
| Bsu36I | CCTNAGG | 1 cut(s) | 873 |
| BsuRI | GGCC | 7 cut(s) | 366, 382, 402, 637, 922, 1028, 1044 |
| BsuTUI | ATCGAT | 1 cut(s) | 1155 |
| BtgZI | GCGATG | 1 cut(s) | 292 |
| BtsCI | GGATG | 8 cut(s) | 105, 742, 787, 841, 1504, 1639, 1794, 1849 |
| BtsIMutI | CAGTG | 3 cut(s) | 1275, 1724, 1845 |
| BveI | ACCTGC | 1 cut(s) | 378 |
| Cac8I | GCNNGC | 4 cut(s) | 542, 576, 924, 1649 |
| CfoI | GCGC | 2 cut(s) | 597, 1520 |
| Cfr13I | GGNCC | 4 cut(s) | 185, 364, 920, 1364 |
| ClaI | ATCGAT | 1 cut(s) | 1155 |
| CseI | GACGC | 4 cut(s) | 376, 481, 826, 1589 |
| Csp6I | GTAC | 3 cut(s) | 517, 562, 1273 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 1821 |
| CviAII | CATG | 3 cut(s) | 916, 1522, 1661 |
| CviQI | GTAC | 3 cut(s) | 517, 562, 1273 |
| DdeI | CTNAG | 4 cut(s) | 23, 873, 1064, 1101 |
| DpnI | GATC | 3 cut(s) | 1627, 1658, 1689 |
| DpnII | GATC | 3 cut(s) | 1625, 1656, 1687 |
| EaeI | YGGCCR | 2 cut(s) | 400, 1042 |
| EagI | CGGCCG | 1 cut(s) | 400 |
| Eam1104I | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 644, 686, 794 |
| EarI | CTCTTC | 7 cut(s) | 20, 42, 50, 74, 644, 686, 794 |
| Ecl136II | GAGCTC | 1 cut(s) | 442 |
| EclXI | CGGCCG | 1 cut(s) | 400 |
| Eco130I | CCWWGG | 1 cut(s) | 1629 |
| Eco24I | GRGCYC | 1 cut(s) | 444 |
| Eco47I | GGWCC | 2 cut(s) | 185, 1364 |
| Eco52I | CGGCCG | 1 cut(s) | 400 |
| Eco53kI | GAGCTC | 1 cut(s) | 442 |
| Eco57I | CTGAAG | 2 cut(s) | 274, 1704 |
| Eco81I | CCTNAGG | 1 cut(s) | 873 |
| Eco88I | CYCGRG | 1 cut(s) | 347 |
| Eco91I | GGTNACC | 1 cut(s) | 4 |
| EcoICRI | GAGCTC | 1 cut(s) | 442 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 197 |
| EcoO109I | RGGNCCY | 1 cut(s) | 920 |
| EcoO65I | GGTNACC | 1 cut(s) | 4 |
| EcoRI | GAATTC | 1 cut(s) | 1279 |
| EcoRII | CCWGG | 3 cut(s) | 618, 882, 1777 |
| EcoT14I | CCWWGG | 1 cut(s) | 1629 |
| EcoT22I | ATGCAT | 1 cut(s) | 1603 |
| EcoT38I | GRGCYC | 1 cut(s) | 444 |
| ErhI | CCWWGG | 1 cut(s) | 1629 |
| Esp3I | CGTCTC | 2 cut(s) | 127, 468 |
| FaeI | CATG | 3 cut(s) | 919, 1525, 1664 |
| FaqI | GGGAC | 2 cut(s) | 308, 392 |
| FatI | CATG | 3 cut(s) | 915, 1521, 1660 |
| FauI | CCCGC | 3 cut(s) | 334, 590, 1334 |
| Fnu4HI | GCNGC | 7 cut(s) | 312, 355, 511, 545, 1187, 1383, 1584 |
| FokI | GGATG | 7 cut(s) | 92, 749, 794, 848, 1511, 1646, 1801 |
| FriOI | GRGCYC | 1 cut(s) | 444 |
| Fsp4HI | GCNGC | 7 cut(s) | 312, 355, 511, 545, 1187, 1383, 1584 |
| FspBI | CTAG | 2 cut(s) | 1262, 1595 |
| GlaI | GCGC | 2 cut(s) | 596, 1519 |
| GluI | GCNGC | 7 cut(s) | 312, 355, 511, 545, 1187, 1383, 1584 |
| GsaI | CCCAGC | 2 cut(s) | 779, 1206 |
| GsuI | CTGGAG | 2 cut(s) | 743, 1377 |
| HaeII | RGCGCY | 1 cut(s) | 1521 |
| HaeIII | GGCC | 7 cut(s) | 366, 382, 402, 637, 922, 1028, 1044 |
| HapII | CCGG | 4 cut(s) | 361, 411, 700, 1691 |
| HgaI | GACGC | 4 cut(s) | 376, 481, 826, 1589 |
| HhaI | GCGC | 2 cut(s) | 597, 1520 |
| Hin1I | GRCGYC | 1 cut(s) | 492 |
| Hin1II | CATG | 3 cut(s) | 919, 1525, 1664 |
| Hin6I | GCGC | 2 cut(s) | 595, 1518 |
| HinP1I | GCGC | 2 cut(s) | 595, 1518 |
| HincII | GTYRAC | 1 cut(s) | 1258 |
| HindII | GTYRAC | 1 cut(s) | 1258 |
| HindIII | AAGCTT | 1 cut(s) | 286 |
| HpaII | CCGG | 4 cut(s) | 361, 411, 700, 1691 |
| HphI | GGTGA | 5 cut(s) | 670, 692, 719, 1117, 1573 |
| Hpy166II | GTNNAC | 3 cut(s) | 517, 1258, 1273 |
| Hpy188I | TCNGA | 7 cut(s) | 26, 320, 529, 615, 1137, 1710, 1732 |
| Hpy188III | TCNNGA | 7 cut(s) | 164, 349, 1112, 1284, 1356, 1595, 1691 |
| Hpy8I | GTNNAC | 3 cut(s) | 517, 1258, 1273 |
| Hpy99I | CGWCG | 7 cut(s) | 281, 419, 473, 497, 533, 539, 820 |
| HpyAV | CCTTC | 8 cut(s) | 677, 697, 746, 760, 889, 1001, 1032, 1497 |
| HpyCH4III | ACNGT | 6 cut(s) | 338, 843, 1039, 1141, 1270, 1728 |
| HpyCH4IV | ACGT | 1 cut(s) | 584 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 363, 379, 399, 826, 884, 1326, 1379, 1526 |
| HpyF3I | CTNAG | 4 cut(s) | 23, 873, 1064, 1101 |
| HpySE526I | ACGT | 1 cut(s) | 584 |
| Hsp92I | GRCGYC | 1 cut(s) | 492 |
| Hsp92II | CATG | 3 cut(s) | 919, 1525, 1664 |
| HspAI | GCGC | 2 cut(s) | 595, 1518 |
| Kpn2I | TCCGGA | 1 cut(s) | 1690 |
| Kzo9I | GATC | 3 cut(s) | 1625, 1656, 1687 |
| LguI | GCTCTTC | 1 cut(s) | 20 |
| LmnI | GCTCC | 3 cut(s) | 93, 447, 1005 |
| Lsp1109I | GCAGC | 3 cut(s) | 1198, 1394, 1570 |
| LweI | GCATC | 4 cut(s) | 932, 1147, 1588, 1808 |
| MaeI | CTAG | 2 cut(s) | 1262, 1595 |
| MaeII | ACGT | 1 cut(s) | 584 |
| MaeIII | GTNAC | 4 cut(s) | 4, 458, 658, 1219 |
| MalI | GATC | 3 cut(s) | 1627, 1658, 1689 |
| MboI | GATC | 3 cut(s) | 1625, 1656, 1687 |
| MflI | RGATCY | 2 cut(s) | 1625, 1687 |
| MhlI | GDGCHC | 2 cut(s) | 206, 444 |
| MluCI | AATT | 7 cut(s) | 62, 158, 607, 1279, 1567, 1611, 1640 |
| MlyI | GAGTC | 4 cut(s) | 330, 414, 584, 946 |
| MmeI | TCCRAC | 1 cut(s) | 552 |
| Mph1103I | ATGCAT | 1 cut(s) | 1603 |
| MroI | TCCGGA | 1 cut(s) | 1690 |
| MroXI | GAANNNNTTC | 1 cut(s) | 584 |
| MseI | TTAA | 3 cut(s) | 1482, 1491, 1616 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1665 |
| MspA1I | CMGCKG | 1 cut(s) | 547 |
| MspI | CCGG | 4 cut(s) | 361, 411, 700, 1691 |
| MspR9I | CCNGG | 5 cut(s) | 362, 411, 620, 884, 1779 |
| MvaI | CCWGG | 3 cut(s) | 620, 884, 1779 |
| MvnI | CGCG | 6 cut(s) | 387, 513, 554, 595, 597, 820 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 363, 379, 399, 826, 884, 1326, 1379, 1526 |
| NciI | CCSGG | 2 cut(s) | 362, 411 |
| NdeII | GATC | 3 cut(s) | 1625, 1656, 1687 |
| NlaIII | CATG | 3 cut(s) | 919, 1525, 1664 |
| NlaIV | GGNNCC | 6 cut(s) | 187, 205, 365, 617, 698, 921 |
| NmeAIII | GCCGAG | 2 cut(s) | 428, 463 |
| NmuCI | GTSAC | 3 cut(s) | 4, 658, 1219 |
| NsiI | ATGCAT | 1 cut(s) | 1603 |
| PaeR7I | CTCGAG | 1 cut(s) | 347 |
| PaqCI | CACCTGC | 1 cut(s) | 378 |
| PciSI | GCTCTTC | 1 cut(s) | 20 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 255, 435, 525, 537 |
| PdmI | GAANNNNTTC | 1 cut(s) | 584 |
| PfeI | GAWTC | 7 cut(s) | 99, 1108, 1132, 1207, 1420, 1671, 1719 |
| PflFI | GACNNNGTC | 1 cut(s) | 419 |
| PkrI | GCNGC | 7 cut(s) | 313, 356, 512, 546, 1188, 1384, 1585 |
| PleI | GAGTC | 4 cut(s) | 329, 413, 584, 946 |
| PpsI | GAGTC | 4 cut(s) | 329, 413, 584, 946 |
| Psp124BI | GAGCTC | 1 cut(s) | 444 |
| Psp6I | CCWGG | 3 cut(s) | 618, 882, 1777 |
| PspEI | GGTNACC | 1 cut(s) | 4 |
| PspFI | CCCAGC | 2 cut(s) | 775, 1202 |
| PspGI | CCWGG | 3 cut(s) | 618, 882, 1777 |
| PspN4I | GGNNCC | 6 cut(s) | 187, 205, 365, 617, 698, 921 |
| PspPI | GGNCC | 4 cut(s) | 185, 364, 920, 1364 |
| PstI | CTGCAG | 2 cut(s) | 1384, 1588 |
| PsuI | RGATCY | 2 cut(s) | 1625, 1687 |
| PsyI | GACNNNGTC | 1 cut(s) | 419 |
| RsaI | GTAC | 3 cut(s) | 518, 563, 1274 |
| RsaNI | GTAC | 3 cut(s) | 517, 562, 1273 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1665 |
| SacI | GAGCTC | 1 cut(s) | 444 |
| SapI | GCTCTTC | 1 cut(s) | 20 |
| SaqAI | TTAA | 3 cut(s) | 1482, 1491, 1616 |
| SatI | GCNGC | 7 cut(s) | 312, 355, 511, 545, 1187, 1383, 1584 |
| Sau3AI | GATC | 3 cut(s) | 1625, 1656, 1687 |
| Sau96I | GGNCC | 4 cut(s) | 185, 364, 920, 1364 |
| SchI | GAGTC | 4 cut(s) | 330, 414, 584, 946 |
| ScrFI | CCNGG | 5 cut(s) | 362, 411, 620, 884, 1779 |
| SduI | GDGCHC | 2 cut(s) | 206, 444 |
| SfaNI | GCATC | 4 cut(s) | 932, 1147, 1588, 1808 |
| SfcI | CTRYAG | 3 cut(s) | 1174, 1380, 1584 |
| Sfr274I | CTCGAG | 1 cut(s) | 347 |
| SinI | GGWCC | 2 cut(s) | 185, 1364 |
| SlaI | CTCGAG | 1 cut(s) | 347 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1665 |
| SmlI | CTYRAG | 1 cut(s) | 347 |
| SmoI | CTYRAG | 1 cut(s) | 347 |
| Sse9I | AATT | 7 cut(s) | 62, 158, 607, 1279, 1567, 1611, 1640 |
| SspMI | CTAG | 2 cut(s) | 1262, 1595 |
| SstI | GAGCTC | 1 cut(s) | 444 |
| StyD4I | CCNGG | 5 cut(s) | 360, 409, 618, 882, 1777 |
| StyI | CCWWGG | 1 cut(s) | 1629 |
| TaaI | ACNGT | 6 cut(s) | 338, 843, 1039, 1141, 1270, 1728 |
| TaiI | ACGT | 1 cut(s) | 587 |
| TasI | AATT | 7 cut(s) | 62, 158, 607, 1279, 1567, 1611, 1640 |
| TatI | WGTACW | 2 cut(s) | 561, 1272 |
| TauI | GCSGC | 4 cut(s) | 314, 357, 513, 547 |
| TfiI | GAWTC | 7 cut(s) | 99, 1108, 1132, 1207, 1420, 1671, 1719 |
| Tru1I | TTAA | 3 cut(s) | 1482, 1491, 1616 |
| Tru9I | TTAA | 3 cut(s) | 1482, 1491, 1616 |
| TscAI | CASTG | 3 cut(s) | 1275, 1731, 1845 |
| TseFI | GTSAC | 3 cut(s) | 4, 658, 1219 |
| TseI | GCWGC | 3 cut(s) | 1186, 1382, 1583 |
| Tsp45I | GTSAC | 3 cut(s) | 4, 658, 1219 |
| TspDTI | ATGAA | 8 cut(s) | 753, 852, 908, 992, 1086, 1501, 1580, 1650 |
| TspGWI | ACGGA | 2 cut(s) | 109, 486 |
| TspRI | CASTG | 3 cut(s) | 1275, 1731, 1845 |
| Tth111I | GACNNNGTC | 1 cut(s) | 419 |
| VpaK11BI | GGWCC | 2 cut(s) | 185, 1364 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 197 |
| XapI | RAATTY | 6 cut(s) | 62, 607, 1279, 1567, 1611, 1640 |
| XbaI | TCTAGA | 1 cut(s) | 1594 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1555 |
| XhoI | CTCGAG | 1 cut(s) | 347 |
| XmnI | GAANNNNTTC | 1 cut(s) | 584 |
| XspI | CTAG | 2 cut(s) | 1262, 1595 |
| Zsp2I | ATGCAT | 1 cut(s) | 1603 |