FvH4_3g21210

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011880
Physical Location & Seq
Forward (+)
14214673 .. 14219820
5148 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21210.t1

Sequence Viewer

Length: 1809 bp
ATGGTCGCCAACCTCTTCCTCATCAGACCTCACTCTCTTCCCCTCTTCACTCTCCCCTCAAAATTCCTTTTCCCCAATCCACCCTCGCTCCCCAAAAGATTCCCCATCCTCTCCCTCTCCTTCTCCACCCACCCCCAAATCCCAGACCCGGACCTCTCCTATGGACCCTCCCTCCACAAGGGCACCAAGCCCCCACTCCCCATTCCTCTCTCCACCGACCAACCAATCGAAGAAGCCCTAATCGATGAAGAGAGCTTCACTAGGGTTTTCAACATCGCCGCCCTCCGAGTCCCCGCAAAAGACTGTTTTGCCCTCGAGAGCCGCCTCCGGGGCCACCTCCTCAACTGGCCCCGCGTCCGCAACATCGGCCGGGTCCCTGGCGACGAAGTGGAAGACGAAATCGCCGAGCTTCTCGCTAATAATCAAAACGGCGAGGAGGAAGACCAAAACGGCGTCGTTTCGCTGAACCGCCGCGTGTACGGGAAATCCGAGGGCGACGGCGAGCCGCTGAGCCCGGTGCTGTACAGAGAGAAGCTGGCGAAGACGTTTGACTCGCGCGGGTATGTGAATTTTCGGAACCTGGCGAAGATGTCGAGGCCGAACCAGAAGAAGAGGAGGGCGGCGGAGGGAGGTGACGGCGGTGAAGGGAGGAGGAGGAACCGGAAGAGTGAGTTTTCGGTGCTGGAGGTTGTGGAGGATGAAGATGAGGAAGGAGAGGACTTGAAGGGTTTGCTGGGGGATGCGTTTCAGGGGAGGAAGAAATGGAGGGGTTCGACGCGGTTGTTGCTGTTGGATGAAAGGTATGGGGATAAAGGAGTGGAGGAGTTGCCTCGGGCAATCCAGGCAGTGGTGAAGGAAGATACTGGGAAAAGTATGAGGCCGACCATTGAGCTTGTGAGATGCAAATTGACTCTTTTTTATGATTACTGGCAGATGAATGAGATATTGGAGGCATTGCTGCCCGAAGGTATGATTGTTCCTTCAGCCTTTGAAACAGTTGGCCATATTGCCCACCTAAACCTGAGAGATGAACACTTACCTTACAGAAAGTTTATAGCTAAGGTGATTCTTGATAAAAATAAGCCAAAGATTCAGACTGTTGTAAATAAAATCGATGTCATTCAAAATGACTATAGAACAATGCAGCTTGAAGTTTTAGCTGGGAATCATTCGCTTGTGACAAAAGTAGTTGAGAATGGTTTGCGATTTCAGGTTGACCTAGCAACAGTGTACTGGAATTCCAGACTTGCAACAGAACGACAAAGGCTTTTGAACAGCTTTACCCGCAATGATGTTGTGTCTGATGTTTTCTCTGGAGTTGGTCCAATAGCTATCTCTGCAGCAAAGATTGTAAAACGAGTTTATGCCAATGATTTGAATCCCCATGCTGTTGAATATCTGCAAAGAAATTGTGGTCTCAACAAACTCGATAAGAAGGTTAAGGTCTTCAATATGGATGGAAGGAGGTTCATAAGAGCCATGTTTGCAAGTGATAAAGCTAAATCTATCACACAAGTGGTGATGAATTTGCCAAAGGATGCTGCAGAGTATCTAGATGCATTTCGGGGAATTTTAAGAGATAGATCCAAGGATGAAGAATTTAACTTGCCAATGATCCATGTTTATGGATTCTCCAAAGCTGAAGATCCTGACTTTGACTTCCATCAGAGAATAAGAATCGCATTGGCAGAAGTGGCAGTAGATGTAGAAATGCGTAGGGTTCGCCTTGTTGCACCAGGAAAATGGATGTTGTGCGCATCATTTATCCTCCCAAAAAATGTAGCTTTTGCAAATCCAGTGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Met_10

Protein Analysis

603

Amino Acids

68.35

Weight (kDa)

8.72

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TYW2_N_2 PF25133 312 - 390 4.2e-20 TYW2 N-terminal domain
TRM5-TYW2_MTfase PF02475 394 - 529 3.9e-31 TRM5/TYW2 methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011880)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27340 AT4G27340 AT4G27340
fragaria_vesca FvH4_3g21210
malus_domestica MD03G1217000.v1.1
prunus_persica Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1 Prupe.4G192400_v2.0.a1
pyrus_communis pycom03g16620
rosa_chinensis RchiOBHm_Chr5g0036281
rosa_laevigata RLG00000033683
rosa_multiflora Rmu_sc0000247.1_g000072
rosa_roxburghii Rroxscaffold_1G00044510
rosa_rugosa Rorug05G0154500
rosa_samantha Rh5AG248200 Rh5BG248800 Rh5CG280100 Rh5DG257500
rosa_wichuraiana Rw5G022770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1756
AccB1I GGYRCC 1 cut(s) 182
AccB7I CCANNNNNTGG 1 cut(s) 847
AccII CGCG 5 cut(s) 354, 474, 556, 558, 778
AclWI GGATC 3 cut(s) 1578, 1609, 1640
AcoI YGGCCR 2 cut(s) 367, 1000
AcsI RAATTY 6 cut(s) 62, 568, 1237, 1525, 1569, 1598
AcuI CTGAAG 2 cut(s) 966, 1662
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 479, 524, 1232
AfiI CCNNNNNNNGG 4 cut(s) 148, 178, 328, 847
AgsI TTSAA 9 cut(s) 271, 724, 992, 1124, 1151, 1273, 1378, 1394, 1450
AjnI CCWGG 4 cut(s) 376, 579, 840, 1735
AjuI GAANNNNNNNTTGG 2 cut(s) 929, 961
AleI CACNNNNGTG 1 cut(s) 1514
Alw26I GTCTC 1 cut(s) 1421
AlwI GGATC 3 cut(s) 1578, 1609, 1640
Ama87I CYCGRG 2 cut(s) 314, 831
AoxI GGCC 6 cut(s) 331, 347, 367, 596, 878, 1000
ApeKI GCWGC 4 cut(s) 958, 1144, 1340, 1541
ApoI RAATTY 6 cut(s) 62, 568, 1237, 1525, 1569, 1598
AspLEI GCGC 2 cut(s) 558, 1757
AspS9I GGNCC 6 cut(s) 151, 164, 331, 348, 373, 1322
AsuC2I CCSGG 4 cut(s) 149, 329, 371, 515
AsuHPI GGTGA 5 cut(s) 644, 653, 862, 1075, 1531
AvaI CYCGRG 2 cut(s) 314, 831
AvaII GGWCC 4 cut(s) 151, 164, 373, 1322
BaeGI GKGCMC 1 cut(s) 185
BalI TGGCCA 1 cut(s) 1002
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 515
BbsI GAAGAC 4 cut(s) 399, 447, 548, 1438
BbvI GCAGC 4 cut(s) 945, 1156, 1352, 1528
BccI CCATC 3 cut(s) 113, 1451, 1671
BceAI ACGGC 4 cut(s) 445, 466, 514, 652
BciT130I CCWGG 4 cut(s) 378, 581, 842, 1737
BcnI CCSGG 4 cut(s) 149, 329, 371, 515
BcoDI GTCTC 1 cut(s) 1421
BfaI CTAG 3 cut(s) 261, 1220, 1553
BfmI CTRYAG 3 cut(s) 1132, 1338, 1542
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 9 cut(s) 279, 322, 472, 506, 621, 959, 1145, 1341, 1542
BlpI GCTNAGC 1 cut(s) 509
BlsI GCNGC 9 cut(s) 280, 323, 473, 507, 622, 960, 1146, 1342, 1543
Bme1390I CCNGG 8 cut(s) 149, 329, 371, 378, 515, 581, 842, 1737
Bme18I GGWCC 4 cut(s) 151, 164, 373, 1322
BmeT110I CYCGRG 2 cut(s) 314, 831
BmgT120I GGNCC 6 cut(s) 151, 164, 331, 348, 373, 1322
BmiI GGNNCC 8 cut(s) 166, 184, 332, 350, 374, 375, 578, 659
BmrFI CCNGG 8 cut(s) 149, 329, 371, 378, 515, 581, 842, 1737
BmrI ACTGGG 1 cut(s) 873
BmsI GCATC 5 cut(s) 730, 890, 1528, 1546, 1766
BmuI ACTGGG 1 cut(s) 873
BpiI GAAGAC 4 cut(s) 399, 447, 548, 1438
BpmI CTGGAG 2 cut(s) 704, 1335
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu1102I GCTNAGC 1 cut(s) 509
BpuMI CCSGG 4 cut(s) 149, 329, 371, 515
Bsa29I ATCGAT 2 cut(s) 243, 1113
BsaBI GATNNNNATC 1 cut(s) 1377
BsaHI GRCGYC 1 cut(s) 453
BsaI GGTCTC 1 cut(s) 1421
BsaJI CCNNGG 5 cut(s) 328, 376, 489, 830, 1587
BsaWI WCCGGW 1 cut(s) 660
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 156, 186
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 178, 328, 847
Bse1I ACTGG 5 cut(s) 350, 868, 932, 1238, 1796
Bse3DI GCAATG 2 cut(s) 953, 1294
Bse8I GATNNNNATC 1 cut(s) 1377
BseBI CCWGG 4 cut(s) 378, 581, 842, 1737
BseCI ATCGAT 2 cut(s) 243, 1113
BseDI CCNNGG 5 cut(s) 328, 376, 489, 830, 1587
BseGI GGATG 8 cut(s) 105, 703, 745, 799, 1462, 1543, 1597, 1752
BseJI GATNNNNATC 1 cut(s) 1377
BseLI CCNNNNNNNGG 4 cut(s) 148, 178, 328, 847
BseMI GCAATG 2 cut(s) 953, 1294
BseMII CTCAG 2 cut(s) 500, 1013
BseNI ACTGG 5 cut(s) 350, 868, 932, 1238, 1796
BseRI GAGGAG 6 cut(s) 329, 449, 628, 664, 667, 836
BseSI GKGCMC 1 cut(s) 185
BseX3I CGGCCG 1 cut(s) 367
BseXI GCAGC 4 cut(s) 945, 1156, 1352, 1528
BseYI CCCAGC 2 cut(s) 733, 1160
Bsh1236I CGCG 5 cut(s) 354, 474, 556, 558, 778
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 6 cut(s) 333, 349, 369, 598, 880, 1002
BshNI GGYRCC 1 cut(s) 182
BshVI ATCGAT 2 cut(s) 243, 1113
BsiEI CGRYCG 1 cut(s) 370
BsiHKCI CYCGRG 2 cut(s) 314, 831
BsiSI CCGG 5 cut(s) 149, 328, 370, 515, 661
BslFI GGGAC 2 cut(s) 275, 359
BslI CCNNNNNNNGG 4 cut(s) 148, 178, 328, 847
BsmAI GTCTC 1 cut(s) 1421
BsmFI GGGAC 2 cut(s) 275, 359
BsnI GGCC 6 cut(s) 333, 349, 369, 598, 880, 1002
Bso31I GGTCTC 1 cut(s) 1421
BsoBI CYCGRG 2 cut(s) 314, 831
Bsp1286I GDGCHC 2 cut(s) 185, 515
Bsp1407I TGTACA 1 cut(s) 522
Bsp143I GATC 3 cut(s) 1583, 1614, 1645
Bsp1720I GCTNAGC 1 cut(s) 509
BspANI GGCC 6 cut(s) 333, 349, 369, 598, 880, 1002
BspCNI CTCAG 2 cut(s) 501, 1014
BspDI ATCGAT 2 cut(s) 243, 1113
BspFNI CGCG 5 cut(s) 354, 474, 556, 558, 778
BspLI GGNNCC 8 cut(s) 166, 184, 332, 350, 374, 375, 578, 659
BspMAI CTGCAG 2 cut(s) 1342, 1546
BspPI GGATC 3 cut(s) 1578, 1609, 1640
BspT107I GGYRCC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 1421
BsrDI GCAATG 2 cut(s) 953, 1294
BsrGI TGTACA 1 cut(s) 522
BsrI ACTGG 5 cut(s) 350, 868, 932, 1238, 1796
BssECI CCNNGG 5 cut(s) 328, 376, 489, 830, 1587
BssMI GATC 3 cut(s) 1583, 1614, 1645
BssNI GRCGYC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 1587
Bst2UI CCWGG 4 cut(s) 378, 581, 842, 1737
Bst4CI ACNGT 4 cut(s) 305, 997, 1099, 1228
Bst6I CTCTTC 6 cut(s) 20, 42, 50, 243, 605, 659
BstACI GRCGYC 1 cut(s) 453
BstAUI TGTACA 1 cut(s) 522
BstC8I GCNNGC 2 cut(s) 503, 537
BstDEI CTNAG 3 cut(s) 509, 1022, 1059
BstENI CCTNNNNNAGG 1 cut(s) 176
BstF5I GGATG 8 cut(s) 105, 703, 745, 799, 1462, 1543, 1597, 1752
BstFNI CGCG 5 cut(s) 354, 474, 556, 558, 778
BstHHI GCGC 2 cut(s) 558, 1757
BstKTI GATC 3 cut(s) 1586, 1617, 1648
BstMAI GTCTC 1 cut(s) 1421
BstMBI GATC 3 cut(s) 1583, 1614, 1645
BstMCI CGRYCG 1 cut(s) 370
BstMWI GCNNNNNNNGC 8 cut(s) 330, 366, 784, 842, 1284, 1337, 1484, 1694
BstNI CCWGG 4 cut(s) 378, 581, 842, 1737
BstSCI CCNGG 8 cut(s) 147, 327, 369, 376, 513, 579, 840, 1735
BstSFI CTRYAG 3 cut(s) 1132, 1338, 1542
BstSLI GKGCMC 1 cut(s) 185
BstUI CGCG 5 cut(s) 354, 474, 556, 558, 778
BstV1I GCAGC 4 cut(s) 945, 1156, 1352, 1528
BstV2I GAAGAC 4 cut(s) 399, 447, 548, 1438
BstX2I RGATCY 2 cut(s) 1583, 1645
BstXI CCANNNNNNTGG 2 cut(s) 1625, 1743
BstYI RGATCY 2 cut(s) 1583, 1645
BstZI CGGCCG 1 cut(s) 367
Bsu15I ATCGAT 2 cut(s) 243, 1113
BsuRI GGCC 6 cut(s) 333, 349, 369, 598, 880, 1002
BsuTUI ATCGAT 2 cut(s) 243, 1113
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 8 cut(s) 105, 703, 745, 799, 1462, 1543, 1597, 1752
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 3 cut(s) 852, 1233, 1803
Cac8I GCNNGC 2 cut(s) 503, 537
CfoI GCGC 2 cut(s) 558, 1757
Cfr13I GGNCC 6 cut(s) 151, 164, 331, 348, 373, 1322
ClaI ATCGAT 2 cut(s) 243, 1113
CseI GACGC 3 cut(s) 343, 442, 784
Csp6I GTAC 3 cut(s) 478, 523, 1231
CspCI CAANNNNNGTGG 1 cut(s) 1779
CviAII CATG 3 cut(s) 1385, 1480, 1619
CviQI GTAC 3 cut(s) 478, 523, 1231
DdeI CTNAG 3 cut(s) 509, 1022, 1059
DpnI GATC 3 cut(s) 1585, 1616, 1647
DpnII GATC 3 cut(s) 1583, 1614, 1645
EaeI YGGCCR 2 cut(s) 367, 1000
EagI CGGCCG 1 cut(s) 367
Eam1104I CTCTTC 6 cut(s) 20, 42, 50, 243, 605, 659
EarI CTCTTC 6 cut(s) 20, 42, 50, 243, 605, 659
EciI GGCGGA 1 cut(s) 638
EclXI CGGCCG 1 cut(s) 367
Eco130I CCWWGG 1 cut(s) 1587
Eco24I GRGCYC 1 cut(s) 515
Eco31I GGTCTC 1 cut(s) 1421
Eco47I GGWCC 4 cut(s) 151, 164, 373, 1322
Eco52I CGGCCG 1 cut(s) 367
Eco57I CTGAAG 2 cut(s) 966, 1662
Eco88I CYCGRG 2 cut(s) 314, 831
EcoNI CCTNNNNNAGG 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 4 cut(s) 376, 579, 840, 1735
EcoT14I CCWWGG 1 cut(s) 1587
EcoT22I ATGCAT 1 cut(s) 1561
EcoT38I GRGCYC 1 cut(s) 515
ErhI CCWWGG 1 cut(s) 1587
FaeI CATG 3 cut(s) 1388, 1483, 1622
FaqI GGGAC 2 cut(s) 275, 359
FatI CATG 3 cut(s) 1384, 1479, 1618
FauI CCCGC 4 cut(s) 301, 359, 551, 1292
Fnu4HI GCNGC 9 cut(s) 279, 322, 472, 506, 621, 959, 1145, 1341, 1542
FokI GGATG 8 cut(s) 92, 710, 752, 806, 1469, 1550, 1604, 1759
FriOI GRGCYC 1 cut(s) 515
Fsp4HI GCNGC 9 cut(s) 279, 322, 472, 506, 621, 959, 1145, 1341, 1542
FspAI RTGCGCAY 1 cut(s) 1756
FspBI CTAG 3 cut(s) 261, 1220, 1553
FspI TGCGCA 1 cut(s) 1756
GlaI GCGC 2 cut(s) 557, 1756
GluI GCNGC 9 cut(s) 279, 322, 472, 506, 621, 959, 1145, 1341, 1542
GsaI CCCAGC 2 cut(s) 737, 1164
GsuI CTGGAG 2 cut(s) 704, 1335
HaeIII GGCC 6 cut(s) 333, 349, 369, 598, 880, 1002
HapII CCGG 5 cut(s) 149, 328, 370, 515, 661
HgaI GACGC 3 cut(s) 343, 442, 784
HhaI GCGC 2 cut(s) 558, 1757
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 3 cut(s) 1388, 1483, 1622
Hin6I GCGC 2 cut(s) 556, 1755
HinP1I GCGC 2 cut(s) 556, 1755
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HpaII CCGG 5 cut(s) 149, 328, 370, 515, 661
HphI GGTGA 5 cut(s) 644, 653, 862, 1075, 1531
Hpy166II GTNNAC 3 cut(s) 478, 1216, 1231
Hpy188I TCNGA 7 cut(s) 26, 287, 490, 576, 1095, 1303, 1668
Hpy188III TCNNGA 6 cut(s) 316, 1070, 1242, 1314, 1553, 1649
Hpy8I GTNNAC 3 cut(s) 478, 1216, 1231
Hpy99I CGWCG 4 cut(s) 386, 458, 500, 778
HpyAV CCTTC 9 cut(s) 130, 638, 704, 718, 847, 959, 990, 1429, 1455
HpyCH4III ACNGT 4 cut(s) 305, 997, 1099, 1228
HpyCH4IV ACGT 1 cut(s) 545
HpyF10VI GCNNNNNNNGC 8 cut(s) 330, 366, 784, 842, 1284, 1337, 1484, 1694
HpyF3I CTNAG 3 cut(s) 509, 1022, 1059
HpySE526I ACGT 1 cut(s) 545
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 3 cut(s) 1388, 1483, 1622
HspAI GCGC 2 cut(s) 556, 1755
KflI GGGWCCC 1 cut(s) 373
Kzo9I GATC 3 cut(s) 1583, 1614, 1645
LmnI GCTCC 1 cut(s) 93
Lsp1109I GCAGC 4 cut(s) 945, 1156, 1352, 1528
LweI GCATC 5 cut(s) 730, 890, 1528, 1546, 1766
MaeI CTAG 3 cut(s) 261, 1220, 1553
MaeII ACGT 1 cut(s) 545
MaeIII GTNAC 2 cut(s) 632, 1177
MalI GATC 3 cut(s) 1585, 1616, 1647
MboI GATC 3 cut(s) 1583, 1614, 1645
MflI RGATCY 2 cut(s) 1583, 1645
MhlI GDGCHC 2 cut(s) 185, 515
MlsI TGGCCA 1 cut(s) 1002
MluCI AATT 8 cut(s) 62, 568, 905, 1237, 1408, 1525, 1569, 1598
MluNI TGGCCA 1 cut(s) 1002
MlyI GAGTC 3 cut(s) 297, 545, 904
MmeI TCCRAC 1 cut(s) 771
Mox20I TGGCCA 1 cut(s) 1002
Mph1103I ATGCAT 1 cut(s) 1561
MscI TGGCCA 1 cut(s) 1002
MseI TTAA 3 cut(s) 1440, 1574, 1602
MslI CAYNNNNRTG 2 cut(s) 1514, 1623
Msp20I TGGCCA 1 cut(s) 1002
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 5 cut(s) 149, 328, 370, 515, 661
MspR9I CCNGG 8 cut(s) 149, 329, 371, 378, 515, 581, 842, 1737
MvaI CCWGG 4 cut(s) 378, 581, 842, 1737
MvnI CGCG 5 cut(s) 354, 474, 556, 558, 778
MwoI GCNNNNNNNGC 8 cut(s) 330, 366, 784, 842, 1284, 1337, 1484, 1694
NciI CCSGG 4 cut(s) 149, 329, 371, 515
NdeII GATC 3 cut(s) 1583, 1614, 1645
NlaIII CATG 3 cut(s) 1388, 1483, 1622
NlaIV GGNNCC 8 cut(s) 166, 184, 332, 350, 374, 375, 578, 659
NmeAIII GCCGAG 1 cut(s) 430
NmuCI GTSAC 2 cut(s) 632, 1177
NsbI TGCGCA 1 cut(s) 1756
NsiI ATGCAT 1 cut(s) 1561
OliI CACNNNNGTG 1 cut(s) 1514
PaeR7I CTCGAG 1 cut(s) 314
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 486
PfeI GAWTC 7 cut(s) 99, 1066, 1090, 1165, 1378, 1629, 1677
PflMI CCANNNNNTGG 1 cut(s) 847
PkrI GCNGC 9 cut(s) 280, 323, 473, 507, 622, 960, 1146, 1342, 1543
PleI GAGTC 3 cut(s) 296, 545, 904
PpsI GAGTC 3 cut(s) 296, 545, 904
PpuMI RGGWCCY 1 cut(s) 373
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 4 cut(s) 376, 579, 840, 1735
PspFI CCCAGC 2 cut(s) 733, 1160
PspGI CCWGG 4 cut(s) 376, 579, 840, 1735
PspN4I GGNNCC 8 cut(s) 166, 184, 332, 350, 374, 375, 578, 659
PspPI GGNCC 6 cut(s) 151, 164, 331, 348, 373, 1322
PspPPI RGGWCCY 1 cut(s) 373
PstI CTGCAG 2 cut(s) 1342, 1546
PsuI RGATCY 2 cut(s) 1583, 1645
RsaI GTAC 3 cut(s) 479, 524, 1232
RsaNI GTAC 3 cut(s) 478, 523, 1231
RseI CAYNNNNRTG 2 cut(s) 1514, 1623
SaqAI TTAA 3 cut(s) 1440, 1574, 1602
SatI GCNGC 9 cut(s) 279, 322, 472, 506, 621, 959, 1145, 1341, 1542
Sau3AI GATC 3 cut(s) 1583, 1614, 1645
Sau96I GGNCC 6 cut(s) 151, 164, 331, 348, 373, 1322
SchI GAGTC 3 cut(s) 297, 545, 904
ScrFI CCNGG 8 cut(s) 149, 329, 371, 378, 515, 581, 842, 1737
SduI GDGCHC 2 cut(s) 185, 515
SfaNI GCATC 5 cut(s) 730, 890, 1528, 1546, 1766
SfcI CTRYAG 3 cut(s) 1132, 1338, 1542
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 4 cut(s) 151, 164, 373, 1322
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 2 cut(s) 1514, 1623
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 8 cut(s) 62, 568, 905, 1237, 1408, 1525, 1569, 1598
SspMI CTAG 3 cut(s) 261, 1220, 1553
StyD4I CCNGG 8 cut(s) 147, 327, 369, 376, 513, 579, 840, 1735
StyI CCWWGG 1 cut(s) 1587
TaaI ACNGT 4 cut(s) 305, 997, 1099, 1228
TaiI ACGT 1 cut(s) 548
TaqI TCGA 7 cut(s) 228, 243, 315, 593, 773, 1113, 1428
TasI AATT 8 cut(s) 62, 568, 905, 1237, 1408, 1525, 1569, 1598
TatI WGTACW 2 cut(s) 522, 1230
TauI GCSGC 5 cut(s) 281, 324, 474, 508, 623
TfiI GAWTC 7 cut(s) 99, 1066, 1090, 1165, 1378, 1629, 1677
Tru1I TTAA 3 cut(s) 1440, 1574, 1602
Tru9I TTAA 3 cut(s) 1440, 1574, 1602
TscAI CASTG 3 cut(s) 852, 1233, 1803
TseFI GTSAC 2 cut(s) 632, 1177
TseI GCWGC 4 cut(s) 958, 1144, 1340, 1541
Tsp45I GTSAC 2 cut(s) 632, 1177
TspDTI ATGAA 8 cut(s) 261, 714, 810, 950, 1044, 1459, 1538, 1608
TspRI CASTG 3 cut(s) 852, 1233, 1803
Van91I CCANNNNNTGG 1 cut(s) 847
VpaK11BI GGWCC 4 cut(s) 151, 164, 373, 1322
XagI CCTNNNNNAGG 1 cut(s) 176
XapI RAATTY 6 cut(s) 62, 568, 1237, 1525, 1569, 1598
XbaI TCTAGA 1 cut(s) 1552
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 3 cut(s) 261, 1220, 1553
Zsp2I ATGCAT 1 cut(s) 1561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.