Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013167

Physical Location & Seq

Genomic Coordinates Reverse (-)

13797616 .. 13803082

Gene Structure 5467 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G27640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3147 bp

ATGGCGCAATCTCTCGAACTTCTGTTGATCCAATTCCTCATGCCAGACAACGATGCTCGGCGTCAAGCAGAGGATCAGATCAAACGTCTTGCCAAAGATCCTCAAGTTGTTCCCGCTCTCGTTCAGCATCTCCGCACCGCTAAAACCCCCAACGTCCGCCAGCTCGCCGCCGTTCTCCTCCGCAAAAGGATTACCGGACATTGGGCTAAGCTTTCTCCTCAGCTTAAACAGCATGTTAAGCAATCCTTAATCGAGAGCATCACTGTGGAGAATAGTCCACCGGTGAGACGTGCGAGTGCTAATGTTGTGAGTGTAGTAGCCAAGTACGCTGTTCCAGCTGGAGAGTGGCCTGATTTATTAACCTTTCTCTTCCAGTGTAGTCAGAGTGCTCAAGAAGATCATCGTGAAGTAGCATTGATCCTTTTCAGCTCTTTGACAGAAACGATTGGGAACACATTTAGGCCATACTTTGCAGACTTGCAGGCTCTTCTTCTCAAGTGTATGCAGGATGAAAGCAGTAGTCGTGTCAGAGTTGCTGCTCTCAAGGCGGTTGGTTCTTTTCTGGAATTCACCAACGACGGAGATGAAGTGGTGAAGTTCCGAGATTTCATTCCCAGTATATTGGATGTATCAAGGAAATGTATTGCATCTGGAGAGGAGGATGTTGCTATACTTGCTTTTGAAATTTTTGATGAGCTGATTGAATCTCCTGCTCCCCTTCTTGGAGATTCTGTCAAAGCAATTGTGCAGTTCTCTCTTGAAGTTTCCTGTAACCAAAATCTGGAAAGTAGCACCCGTCACCAGGCAATCCAAATAGTTTCATGGTTGGCAAAGTATAAATACAATTCCCTTAAAAAACATAAGCTTGTCATACCAATCTTGCAAGTTATGTGCCCTTTACTCGCTGAGTCATCTGATCAAGAGGACGATGATGATCTTGCTCCTGATCGTGCATCTGCTGAAGTTATTGACACTCTAGCTATGAACCTGCCAAAGCATGTATTCCTCCCTGTTCTTGAATTTGCTTCCGTGCATTGTCAAAGTACAAACCTCAAGTTTCGGGAAGCTTCTGTAACAGCTCTTGGTGTTATATCAGAGGGTTGCTTTGATCTGATGAAAGAGAAGCTGGATACTGTTCTTAATATAGTCTTAGGAGCTTTAAGAGACCCTGAGCTAGTGGTTCGAGGGGCTGCATCATTTGCAATTGGCCAATTCGCTGAGCATCTTCAGCCCGAGATCCTATCACACTATCAGAGTGTCCTTCCATGCCTATTGATTGCAATTGAAGATACATCTGAGGAAGTGAAGGAAAAGTCACACTATGCTTTAGCAGCATTCTGTGAGAACATGGGAGAGGAAATTGTTCCGCTGCTTGATCATTTAATGGGGAAGCTTATGGCAGCCCTAGAAAATAGTCCGCGTAATCTACAAGAGACATGCATGTCTGCAATTGGGTCGGTTGCTGCTGCTGCAGAGCAAGCATTCAATCCATACGCTGAGAGGGTTCTAGAGTTGATGAAGTTTTTCATGGTGCTCACCAAAGACGAGGATCTTCGTGCCCGTGCCAGGTCTACTGAGCTTGTAGGGATTGTTGCAATGTCTGTTGGGAGAAAAGGGATGGAGGCCATTTTGCCACCTTTCATTGATGCTGCAATATCAGGATTTGAATTGGAGTTCAGTGAGTTGCGAGAATATACTCATGGATTCTTCAGCAACGTAGCAGAAATATTGGATGACACTTTTGCCCAGTATTTGCCTCGTGTTATGCCATTAGTATTTGCTTCGTGCAATCTTGATGATGGATCTGCAGTGGACATTGATGAGTCAGATGATGAAAATGTCAATGATTTTGGTGGGGTATCCTCTGATGATGATGCTGATGATGAGCCCAGAGTTAGGAACATTAGCGTTAGAACAGGAGTTTTAGATGAAAAGGCAGCTGCAACTCAAGCTCTTGGCCTGTTTGCACTCCACACTAAATCTGCTTTTGCACCCTACCTCGAGGAATCATTGAAGATTATGGACAAACATTCTGCATATTTTCATGAAGATGTTCGGCTTCAAGCAGTTACTGGTTTGAAACATATATTGGCTGCAGCTCATGCTATCTTCCAGACTCATAATGATGGAACTGGAAAGGCAAATGAAATTCTTGATACAGTTATGAATAATTATATCAAAACTATGACTGATGATGACGACAAGGAGGTTGTTGCTCAGGCTTGCATCAGTGTTGCCGATATCATGAAGGATTATGGTTACCCAGCCATTCAAAAGTATTTATCACCCCTTGTTGATGCGACATTGCTACTGCTGACGGAAAAAGCAGCTTGCCAGCAGTTAGAAGATGAGAGTGATATTGATGATGATGATACTGGACATGATGAGGTCCTCATGGATGCAGTTTCTGACCTCCTTCCCGCCTTTGCAAAGTGTATGGGTTCTCAATTTGAACCTGTCTTTGCCCAATTCTTTGAGCCGTTGATGAAATTTGCGAAAGCTTCACGTCCTCCACAGGATAGGACAATGGTAGTTGCTAGTCTTGCTGAGGTTGCTCAAGACATGGGTCTTCCAATCTCTTCCTATGTTGATAGATTAATGCCTTTGGTGCTCAAAGAGTTGGGGTCACCAGAAGCAACCAATAGAAGGAATGCAGCTTTCTGTGTTGGAGAGCTATGCAAAAACGGGGGTGAAACTGCTCTTAAATATTTTGGTGATGTGCTACGCGGAATTTCCCCACTTTTCGGGGATTCAGAACCAGACCTTGCTGTTAGGGATAATGCAGCAGGTGCTACCGCAAGGATGATTGTTGTTCATCCTCAACTAGTCCCGCTAAATCAAGTACTTCCAGTGTTCTTAAGAGGTCTACCTCTAAAGGAAGATCAAGAGGAGTCCATGGCTGTTTACACCTGTATATATTCCCTTGTTTCGTCATCCAATCCACAGATCTTCTCTCATGTTCCGGAGCTGGTTAAAATCTTTGGGCAAGTATTGGAGTCGCCAGTCGAAAAAGTTGAAGTGAAAGCAATTGTAGGCAGAACTTTCTCCCATCTAATCTCGGTTTATGGGAATCAGTTGCAGCCAATAATAAGTAGTCTACCGCCTTCTCAAGCTAACGTTCTCGCTGCGTTTGCATCCACGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0017038 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061608 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT IBN_N Vac14_Fab1_bd

Protein Analysis

1048

Amino Acids

115.23

Weight (kDa)

4.77

Isoelectric Point (pI)

46.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	23 - 87	2.5e-15	Importin-beta N-terminal domain
TPR_IPO5	PF25780	92 - 251	1.1e-33	IPO5-like, TPR repeats
TOR1L1_N	PF24714	320 - 534	4.3e-10	TORTIFOLIA1 N-terminal
TPR_IMB1	PF25574	374 - 515	5.5e-10	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27640
fragaria_vesca	FvH4_5g29150
malus_domestica	MD08G1233900.v1.1 MD08G1234100.v1.1 MD15G1425400.v1.1
prunus_persica	Prupe.1G571100_v2.0.a1 Prupe.1G571100_v2.0.a1
pyrus_communis	pycom08g20240 pycom15g37710
rosa_chinensis	RchiOBHm_Chr7g0221821
rosa_laevigata	RLG00000002119
rosa_multiflora	Rmu_sc0005834.1_g000014
rosa_roxburghii	Rroxscaffold_3G00237670
rosa_rugosa	Rorug07G0203600
rosa_samantha	Rh7BG335300 Rh7CG362100 Rh7DG342400
rosa_wichuraiana	Rw7G029220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2777
AasI	GACNNNNNNGTC	1 cut(s)	1441
AbsI	CCTCGAGG	1 cut(s)	2000
Acc36I	ACCTGC	2 cut(s)	996, 2777
AccB7I	CCANNNNNTGG	1 cut(s)	781
AccBSI	CCGCTC	1 cut(s)	116
AccI	GTMKAC	3 cut(s)	1571, 2866, 3097
AccII	CGCG	2 cut(s)	1420, 2727
AccIII	TCCGGA	1 cut(s)	2962
AclI	AACGTT	1 cut(s)	3117
AclWI	GGATC	7 cut(s)	22, 81, 92, 412, 1231, 1557, 1810
AcoI	YGGCCR	1 cut(s)	1207
AcsI	RAATTY	6 cut(s)	566, 684, 1019, 2148, 2489, 2730
AcuI	CTGAAG	3 cut(s)	981, 1211, 1693
AcyI	GRCGYC	1 cut(s)	61
AfaI	GTAC	3 cut(s)	326, 1045, 2844
AfiI	CCNNNNNNNGG	8 cut(s)	201, 722, 781, 802, 1566, 1896, 2646, 2744
AflII	CTTAAG	1 cut(s)	2857
AgeI	ACCGGT	1 cut(s)	280
AhlI	ACTAGT	1 cut(s)	2824
AjiI	CACGTC	2 cut(s)	290, 2507
AjnI	CCWGG	2 cut(s)	801, 1565
AjuI	GAANNNNNNNTTGG	2 cut(s)	2072, 2104
Alw21I	GWGCWC	3 cut(s)	391, 1536, 2613
Alw26I	GTCTC	3 cut(s)	280, 1158, 1427
AlwI	GGATC	7 cut(s)	22, 81, 92, 412, 1231, 1557, 1810
AlwNI	CAGNNNCTG	2 cut(s)	2072, 2408
Ama87I	CYCGRG	2 cut(s)	1232, 2000
Aor13HI	TCCGGA	1 cut(s)	2962
AoxI	GGCC	5 cut(s)	347, 461, 1207, 1623, 1957
ApoI	RAATTY	6 cut(s)	566, 684, 1019, 2148, 2489, 2730
AseI	ATTAAT	1 cut(s)	2597
AsiGI	ACCGGT	1 cut(s)	280
Asp700I	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	1 cut(s)	2389
AsuHPI	GGTGA	9 cut(s)	295, 562, 604, 791, 1528, 2277, 2619, 2702, 2726
AvaI	CYCGRG	2 cut(s)	1232, 2000
AvaII	GGWCC	1 cut(s)	2389
BaeGI	GKGCMC	2 cut(s)	896, 1561
BalI	TGGCCA	1 cut(s)	1209
BanII	GRGCYC	1 cut(s)	1890
BauI	CACGAG	2 cut(s)	1758, 3139
BbsI	GAAGAC	1 cut(s)	2561
Bbv12I	GWGCWC	3 cut(s)	391, 1536, 2613
BbvCI	CCTCAGC	2 cut(s)	219, 2547
BccI	CCATC	4 cut(s)	1612, 1793, 2120, 3057
BceAI	ACGGC	2 cut(s)	155, 2464
BcgI	CGANNNNNNTGC	2 cut(s)	1437, 1471
BciT130I	CCWGG	2 cut(s)	803, 1567
BciVI	GTATCC	2 cut(s)	1123, 1870
BclI	TGATCA	2 cut(s)	916, 1375
BcoDI	GTCTC	3 cut(s)	280, 1158, 1427
BcuI	ACTAGT	1 cut(s)	2824
BfaI	CTAG	6 cut(s)	977, 1175, 1406, 1508, 2538, 2825
BfmI	CTRYAG	3 cut(s)	1470, 1806, 2094
BfrI	CTTAAG	1 cut(s)	2857
BfuAI	ACCTGC	2 cut(s)	996, 2777
BfuI	GTATCC	2 cut(s)	1123, 1870
BglII	AGATCT	1 cut(s)	2946
BlpI	GCTNAGC	2 cut(s)	207, 1218
BmcAI	AGTACT	1 cut(s)	2844
Bme1390I	CCNGG	2 cut(s)	803, 1567
Bme18I	GGWCC	1 cut(s)	2389
BmeT110I	CYCGRG	2 cut(s)	1232, 2000
BmgBI	CACGTC	2 cut(s)	290, 2507
BmgT120I	GGNCC	1 cut(s)	2389
BmrFI	CCNGG	2 cut(s)	803, 1567
BmrI	ACTGGG	2 cut(s)	609, 1741
BmuI	ACTGGG	2 cut(s)	609, 1741
BoxI	GACNNNNGTC	1 cut(s)	2565
BpiI	GAAGAC	1 cut(s)	2561
BpmI	CTGGAG	2 cut(s)	360, 672
Bpu10I	CCTNAGC	4 cut(s)	219, 1170, 2217, 2547
Bpu1102I	GCTNAGC	2 cut(s)	207, 1218
BpuEI	CTTGAG	8 cut(s)	87, 375, 479, 527, 1037, 1932, 2541, 3093
BsaBI	GATNNNNATC	1 cut(s)	933
BsaHI	GRCGYC	1 cut(s)	61
BsaI	GGTCTC	1 cut(s)	1158
BsaJI	CCNNGG	1 cut(s)	2895
BsaWI	WCCGGW	3 cut(s)	194, 280, 2962
BsaXI	ACNNNNNCTCC	4 cut(s)	573, 603, 717, 747
Bsc4I	CCNNNNNNNGG	8 cut(s)	201, 722, 781, 802, 1566, 1896, 2646, 2744
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	8 cut(s)	373, 615, 1747, 2077, 2137, 2380, 2849, 3002
Bse3DI	GCAATG	2 cut(s)	1602, 2303
Bse8I	GATNNNNATC	1 cut(s)	933
BseAI	TCCGGA	1 cut(s)	2962
BseBI	CCWGG	2 cut(s)	803, 1567
BseDI	CCNNGG	1 cut(s)	2895
BseJI	GATNNNNATC	1 cut(s)	933
BseLI	CCNNNNNNNGG	8 cut(s)	201, 722, 781, 802, 1566, 1896, 2646, 2744
BseMI	GCAATG	2 cut(s)	1602, 2303
BseMII	CTCAG	9 cut(s)	233, 897, 1161, 1209, 1287, 1488, 1566, 2231, 2538
BseNI	ACTGG	8 cut(s)	373, 615, 1747, 2077, 2137, 2380, 2849, 3002
BseRI	GAGGAG	4 cut(s)	167, 207, 671, 2903
BseSI	GKGCMC	2 cut(s)	896, 1561
BseYI	CCCAGC	1 cut(s)	2263
BsgI	GTGCAG	1 cut(s)	767
Bsh1236I	CGCG	2 cut(s)	1420, 2727
BshFI	GGCC	5 cut(s)	349, 463, 1209, 1625, 1959
BshTI	ACCGGT	1 cut(s)	280
BsiHKAI	GWGCWC	3 cut(s)	391, 1536, 2613
BsiHKCI	CYCGRG	2 cut(s)	1232, 2000
BsiSI	CCGG	3 cut(s)	195, 281, 2963
BslFI	GGGAC	1 cut(s)	2813
BslI	CCNNNNNNNGG	8 cut(s)	201, 722, 781, 802, 1566, 1896, 2646, 2744
BsmAI	GTCTC	3 cut(s)	280, 1158, 1427
BsmBI	CGTCTC	1 cut(s)	280
BsmFI	GGGAC	1 cut(s)	2813
BsmI	GAATGC	3 cut(s)	1334, 1481, 2656
BsnI	GGCC	5 cut(s)	349, 463, 1209, 1625, 1959
Bso31I	GGTCTC	1 cut(s)	1158
BsoBI	CYCGRG	2 cut(s)	1232, 2000
Bsp1286I	GDGCHC	6 cut(s)	391, 896, 1536, 1561, 1890, 2613
Bsp13I	TCCGGA	1 cut(s)	2962
Bsp1720I	GCTNAGC	2 cut(s)	207, 1218
Bsp19I	CCATGG	1 cut(s)	2895
BspANI	GGCC	5 cut(s)	349, 463, 1209, 1625, 1959
BspCNI	CTCAG	9 cut(s)	232, 898, 1162, 1210, 1288, 1489, 1567, 2230, 2539
BspEI	TCCGGA	1 cut(s)	2962
BspFNI	CGCG	2 cut(s)	1420, 2727
BspHI	TCATGA	2 cut(s)	2044, 2244
BspMAI	CTGCAG	3 cut(s)	1474, 1810, 2098
BspMI	ACCTGC	2 cut(s)	996, 2777
BspPI	GGATC	7 cut(s)	22, 81, 92, 412, 1231, 1557, 1810
BspQI	GCTCTTC	1 cut(s)	492
BspTI	CTTAAG	1 cut(s)	2857
BspTNI	GGTCTC	1 cut(s)	1158
BsrBI	CCGCTC	1 cut(s)	116
BsrDI	GCAATG	2 cut(s)	1602, 2303
BsrFI	RCCGGY	1 cut(s)	280
BsrI	ACTGG	8 cut(s)	373, 615, 1747, 2077, 2137, 2380, 2849, 3002
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	1 cut(s)	2895
BssNI	GRCGYC	1 cut(s)	61
BssSI	CACGAG	2 cut(s)	1758, 3139
BssT1I	CCWWGG	1 cut(s)	2895
Bst2BI	CACGAG	2 cut(s)	1758, 3139
Bst2UI	CCWGG	2 cut(s)	803, 1567
Bst4CI	ACNGT	3 cut(s)	265, 1135, 2161
Bst6I	CTCTTC	3 cut(s)	374, 492, 2584
BstACI	GRCGYC	1 cut(s)	61
BstAFI	CTTAAG	1 cut(s)	2857
BstAPI	GCANNNNNTGC	3 cut(s)	1199, 2102, 2789
BstC8I	GCNNGC	7 cut(s)	161, 165, 483, 1479, 2224, 2332, 2336
BstDSI	CCRYGG	1 cut(s)	2895
BstEII	GGTNACC	2 cut(s)	2258, 2625
BstFNI	CGCG	2 cut(s)	1420, 2727
BstHHI	GCGC	1 cut(s)	7
BstMAI	GTCTC	3 cut(s)	280, 1158, 1427
BstNI	CCWGG	2 cut(s)	803, 1567
BstNSI	RCATGY	4 cut(s)	236, 1001, 1440, 1444
BstPAI	GACNNNNGTC	1 cut(s)	2565
BstPI	GGTNACC	2 cut(s)	2258, 2625
BstSCI	CCNGG	2 cut(s)	801, 1565
BstSFI	CTRYAG	3 cut(s)	1470, 1806, 2094
BstSLI	GKGCMC	2 cut(s)	896, 1561
BstUI	CGCG	2 cut(s)	1420, 2727
BstV2I	GAAGAC	1 cut(s)	2561
BstX2I	RGATCY	5 cut(s)	97, 1236, 1549, 1802, 2946
BstXI	CCANNNNNNTGG	1 cut(s)	622
BstYI	RGATCY	5 cut(s)	97, 1236, 1549, 1802, 2946
BsuI	GTATCC	2 cut(s)	1123, 1870
BsuRI	GGCC	5 cut(s)	349, 463, 1209, 1625, 1959
BtgI	CCRYGG	1 cut(s)	2895
BtrI	CACGTC	2 cut(s)	290, 2507
BtsI	GCAGTG	1 cut(s)	1815
BtsIMutI	CAGTG	6 cut(s)	261, 380, 1684, 1815, 2236, 2856
BveI	ACCTGC	2 cut(s)	996, 2777
Cac8I	GCNNGC	7 cut(s)	161, 165, 483, 1479, 2224, 2332, 2336
CaiI	CAGNNNCTG	2 cut(s)	2072, 2408
CciI	TCATGA	2 cut(s)	2044, 2244
CfoI	GCGC	1 cut(s)	7
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	1 cut(s)	2389
CseI	GACGC	1 cut(s)	50
Csp6I	GTAC	3 cut(s)	325, 1044, 2843
CspAI	ACCGGT	1 cut(s)	280
CviQI	GTAC	3 cut(s)	325, 1044, 2843
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	1 cut(s)	1207
Eam1104I	CTCTTC	3 cut(s)	374, 492, 2584
EarI	CTCTTC	3 cut(s)	374, 492, 2584
EciI	GGCGGA	1 cut(s)	146
Eco130I	CCWWGG	1 cut(s)	2895
Eco24I	GRGCYC	1 cut(s)	1890
Eco31I	GGTCTC	1 cut(s)	1158
Eco32I	GATATC	1 cut(s)	2242
Eco47I	GGWCC	1 cut(s)	2389
Eco57I	CTGAAG	3 cut(s)	981, 1211, 1693
Eco88I	CYCGRG	2 cut(s)	1232, 2000
Eco91I	GGTNACC	2 cut(s)	2258, 2625
EcoO109I	RGGNCCY	1 cut(s)	2389
EcoO65I	GGTNACC	2 cut(s)	2258, 2625
EcoRI	GAATTC	1 cut(s)	566
EcoRII	CCWGG	2 cut(s)	801, 1565
EcoRV	GATATC	1 cut(s)	2242
EcoT14I	CCWWGG	1 cut(s)	2895
EcoT22I	ATGCAT	1 cut(s)	1442
EcoT38I	GRGCYC	1 cut(s)	1890
ErhI	CCWWGG	1 cut(s)	2895
Esp3I	CGTCTC	1 cut(s)	280
FalI	AAGNNNNNCTT	2 cut(s)	230, 262
FaqI	GGGAC	1 cut(s)	2813
FauI	CCCGC	3 cut(s)	121, 2428, 2838
FbaI	TGATCA	2 cut(s)	916, 1375
FblI	GTMKAC	3 cut(s)	1571, 2866, 3097
FriOI	GRGCYC	1 cut(s)	1890
FspBI	CTAG	6 cut(s)	977, 1175, 1406, 1508, 2538, 2825
GlaI	GCGC	1 cut(s)	6
GsaI	CCCAGC	1 cut(s)	2267
GsuI	CTGGAG	2 cut(s)	360, 672
HaeIII	GGCC	5 cut(s)	349, 463, 1209, 1625, 1959
HapII	CCGG	3 cut(s)	195, 281, 2963
HgaI	GACGC	1 cut(s)	50
HhaI	GCGC	1 cut(s)	7
Hin1I	GRCGYC	1 cut(s)	61
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HindIII	AAGCTT	5 cut(s)	209, 863, 1065, 1391, 2499
HpaII	CCGG	3 cut(s)	195, 281, 2963
HphI	GGTGA	9 cut(s)	295, 562, 604, 791, 1528, 2277, 2619, 2702, 2726
Hpy166II	GTNNAC	6 cut(s)	278, 1572, 1813, 2867, 2905, 3098
Hpy8I	GTNNAC	6 cut(s)	278, 1572, 1813, 2867, 2905, 3098
Hpy99I	CGWCG	1 cut(s)	581
HpyAV	CCTTC	7 cut(s)	728, 1271, 1300, 2242, 2426, 2640, 3114
HpyCH4III	ACNGT	3 cut(s)	265, 1135, 2161
HpyCH4IV	ACGT	6 cut(s)	85, 153, 289, 1716, 2506, 3117
HpySE526I	ACGT	6 cut(s)	85, 153, 289, 1716, 2506, 3117
Hsp92I	GRCGYC	1 cut(s)	61
HspAI	GCGC	1 cut(s)	5
Kpn2I	TCCGGA	1 cut(s)	2962
Ksp22I	TGATCA	2 cut(s)	916, 1375
LguI	GCTCTTC	1 cut(s)	492
LmnI	GCTCC	4 cut(s)	718, 946, 1154, 2965
MaeI	CTAG	6 cut(s)	977, 1175, 1406, 1508, 2538, 2825
MaeII	ACGT	6 cut(s)	85, 153, 289, 1716, 2506, 3117
MaeIII	GTNAC	7 cut(s)	770, 797, 1072, 1314, 2068, 2258, 2625
MbiI	CCGCTC	1 cut(s)	116
MfeI	CAATTG	5 cut(s)	741, 1203, 1281, 1449, 3027
MflI	RGATCY	5 cut(s)	97, 1236, 1549, 1802, 2946
MhlI	GDGCHC	6 cut(s)	391, 896, 1536, 1561, 1890, 2613
MlsI	TGGCCA	1 cut(s)	1209
MluNI	TGGCCA	1 cut(s)	1209
MlyI	GAGTC	5 cut(s)	917, 1832, 2110, 2900, 3005
MmeI	TCCRAC	1 cut(s)	2647
Mox20I	TGGCCA	1 cut(s)	1209
Mph1103I	ATGCAT	1 cut(s)	1442
MroI	TCCGGA	1 cut(s)	2962
MroXI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
MscI	TGGCCA	1 cut(s)	1209
MslI	CAYNNNNRTG	3 cut(s)	263, 2049, 2124
Msp20I	TGGCCA	1 cut(s)	1209
MspA1I	CMGCKG	3 cut(s)	338, 1369, 1940
MspCI	CTTAAG	1 cut(s)	2857
MspI	CCGG	3 cut(s)	195, 281, 2963
MspR9I	CCNGG	2 cut(s)	803, 1567
MunI	CAATTG	5 cut(s)	741, 1203, 1281, 1449, 3027
Mva1269I	GAATGC	3 cut(s)	1334, 1481, 2656
MvaI	CCWGG	2 cut(s)	803, 1567
MvnI	CGCG	2 cut(s)	1420, 2727
NcoI	CCATGG	1 cut(s)	2895
NmeAIII	GCCGAG	1 cut(s)	37
NmuCI	GTSAC	3 cut(s)	797, 1314, 2625
NsiI	ATGCAT	1 cut(s)	1442
NspI	RCATGY	4 cut(s)	236, 1001, 1440, 1444
PaeR7I	CTCGAG	1 cut(s)	2000
PagI	TCATGA	2 cut(s)	2044, 2244
PaqCI	CACCTGC	1 cut(s)	2777
PciSI	GCTCTTC	1 cut(s)	492
PctI	GAATGC	3 cut(s)	1334, 1481, 2656
PdmI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
PfeI	GAWTC	6 cut(s)	704, 728, 1704, 2006, 2750, 3070
PflMI	CCANNNNNTGG	1 cut(s)	781
PinAI	ACCGGT	1 cut(s)	280
PleI	GAGTC	5 cut(s)	916, 1831, 2110, 2899, 3004
PpsI	GAGTC	5 cut(s)	916, 1831, 2110, 2899, 3004
PpuMI	RGGWCCY	1 cut(s)	2389
PshAI	GACNNNNGTC	1 cut(s)	2565
PshBI	ATTAAT	1 cut(s)	2597
Psp1406I	AACGTT	1 cut(s)	3117
Psp5II	RGGWCCY	1 cut(s)	2389
Psp6I	CCWGG	2 cut(s)	801, 1565
PspEI	GGTNACC	2 cut(s)	2258, 2625
PspFI	CCCAGC	1 cut(s)	2263
PspGI	CCWGG	2 cut(s)	801, 1565
PspPI	GGNCC	1 cut(s)	2389
PspPPI	RGGWCCY	1 cut(s)	2389
PspXI	VCTCGAGB	1 cut(s)	2000
PstI	CTGCAG	3 cut(s)	1474, 1810, 2098
PstNI	CAGNNNCTG	2 cut(s)	2072, 2408
PsuI	RGATCY	5 cut(s)	97, 1236, 1549, 1802, 2946
PvuII	CAGCTG	2 cut(s)	338, 1940
RsaI	GTAC	3 cut(s)	326, 1045, 2844
RsaNI	GTAC	3 cut(s)	325, 1044, 2843
RseI	CAYNNNNRTG	3 cut(s)	263, 2049, 2124
SapI	GCTCTTC	1 cut(s)	492
Sau96I	GGNCC	1 cut(s)	2389
ScaI	AGTACT	1 cut(s)	2844
SchI	GAGTC	5 cut(s)	917, 1832, 2110, 2900, 3005
ScrFI	CCNGG	2 cut(s)	803, 1567
SduI	GDGCHC	6 cut(s)	391, 896, 1536, 1561, 1890, 2613
SfcI	CTRYAG	3 cut(s)	1470, 1806, 2094
Sfr274I	CTCGAG	1 cut(s)	2000
SgrAI	CRCCGGYG	1 cut(s)	280
SinI	GGWCC	1 cut(s)	2389
SlaI	CTCGAG	1 cut(s)	2000
SmiMI	CAYNNNNRTG	3 cut(s)	263, 2049, 2124
SpeI	ACTAGT	1 cut(s)	2824
SspI	AATATT	2 cut(s)	1728, 2708
SspMI	CTAG	6 cut(s)	977, 1175, 1406, 1508, 2538, 2825
StyD4I	CCNGG	2 cut(s)	801, 1565
StyI	CCWWGG	1 cut(s)	2895
TaaI	ACNGT	3 cut(s)	265, 1135, 2161
TaiI	ACGT	6 cut(s)	88, 156, 292, 1719, 2509, 3120
TaqI	TCGA	5 cut(s)	15, 252, 1183, 2001, 3006
TatI	WGTACW	2 cut(s)	1043, 2842
TauI	GCSGC	1 cut(s)	170
TfiI	GAWTC	6 cut(s)	704, 728, 1704, 2006, 2750, 3070
TscAI	CASTG	6 cut(s)	268, 380, 1684, 1815, 2236, 2856
TseFI	GTSAC	3 cut(s)	797, 1314, 2625
Tsp45I	GTSAC	3 cut(s)	797, 1314, 2625
TspGWI	ACGGA	3 cut(s)	594, 1018, 2333
TspRI	CASTG	6 cut(s)	268, 380, 1684, 1815, 2236, 2856
Van91I	CCANNNNNTGG	1 cut(s)	781
Vha464I	CTTAAG	1 cut(s)	2857
VpaK11BI	GGWCC	1 cut(s)	2389
VspI	ATTAAT	1 cut(s)	2597
XapI	RAATTY	6 cut(s)	566, 684, 1019, 2148, 2489, 2730
XbaI	TCTAGA	1 cut(s)	1507
XceI	RCATGY	4 cut(s)	236, 1001, 1440, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	342
XhoI	CTCGAG	1 cut(s)	2000
XmiI	GTMKAC	3 cut(s)	1571, 2866, 3097
XmnI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
XspI	CTAG	6 cut(s)	977, 1175, 1406, 1508, 2538, 2825
ZrmI	AGTACT	1 cut(s)	2844
Zsp2I	ATGCAT	1 cut(s)	1442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.