Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0013167

Physical Location & Seq

Genomic Coordinates Reverse (-)

52630995 .. 52640986

Gene Structure 9992 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1425400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGTCGCAGTCACTGGAGCTTTTGCTGATACAATTCCTGATGCCGGACAACGACGCCCGGCGGCAGGCGGAGGACCAGATAAAGCGGTTGGCCAAGGACCCTCAGGTGGTTCCTGCGCTCGTACAGCACCTCCGCACCGCCAAGACGCCGAACGTGCGGCAATTGGCCGCCGTGCTTCTGAGGAAGAAGATCACTGGACACTGGGCCAAGCTCTCTCCTCAGAGTAAACACCTCGTTAAGCAGTCCCTCATTGAGAGCATCACAATGGAGCACAGTCCGCCGGTGAGGAGAGCCAGTGCCAATGTGGTTAGCGTCGTGGCAAAATATGCGGTCCCGGCTGGAGAATGGCCGGATTTGTTGCCCTTTCTGTTTCAATGTAGTCAGAGTGCACAGGAAGAACATAGAGAGGTGGCTTTGATCCTGTTCAGCTCTTTGACTGAAACAATAGGGAATACTTTTCGGCCACATTTCGCAGATTTGCAAGCTCTTCTGTTGAAATGCCTGCAGGATGAGACAAGCAACCGCGTCAGAGTTGCTGCACTCAAGGCTGTAGGGTCTTTTCTGGAATTCACTCATGATGGGGTTGAAGTGGTGAAGTTTCGAGAATTCATACCCAGCATCTTAAATGTTTCAAGACAATGCCTTGCAGCCGGCGAGGAGGATGTTGCTATTATTGCTTTTGAAATTTTTGATGAGTTGATTGAATCTCCCGCACCTCTTCTTGGGGAATCTGTTAAATCCATTGTGCAATTCTCTCTTGAAGTTTGCTCAAGTCAAAGTTTAGAATCTAATACACGTCATCAGGCAGTTCAGATAGTTTCATGGCTAGCAAAGTATAAATCCAATTCCCTCAAAAAGCATAAGCTGGTCATCCCTATTCTGCAAGTTATGTGCCCATTGCTCGCTGAATCAAATGATGAAGATAAGGATGATGATCTTGCTCCAGATCGGGCTGCTGCAGAAGTTATTGATACTATGGCTTTGAACATACCAAAGCATGTTTTCCACCCTGTCTTTGAGTTTTCTTCTTTAAGCAGTCAAAATGCAAATCCAAAATATCGGGAAGCATCTGTTACCGCTTTAGGTGTCATTTCAGAGGGTTGTTTGGAGATGATAAAAGATAAGTTGGATCCAGTTCTTCTTATTGTTTTAGGCGCTCTGAGAGATCCTGAGGAAGTGGTTAGGGGGGCTGCATCATTTGCTTTGGGTCAGTTTGCGGAGCATTTGCAACCTGAAATTGTTTCCCACTACCAAAGTGTGCTTCCTTGCATTTTGAATGCCCTCGAGGATGCATCTGACGAAGTGAAGGAGAAGTCATATTATGCTTTGGCAGCATTTTGTGACAACATGGGTGAGGAAATTCTTCCATTCCTTGATCCGTTGATGGGAAAACTACTGGGGGCCCTCCATAATAGCCCTCGTAATTTGCAGGAGACGTGCATGTCAGCAATTGGTTCAGTTGCATCTGCTGCAGAACAGGCATTCGTTCCCTATGCTGAAAGGGTTTTGGAGTTGATGAAAAATTTCTTGGTGCTTAGTAATGATGAGGATCTTCGTTCACGAGCAAGAGCCACTGAATTAGTTGGAATAGTTGCAATGTGTGTGGGGAGAACTAGGATGGAACCAATTTTACCCCCTTATATAGAAGCTGCAATTTCTGGTTTTGGATTGGAATTCAGTGAACTTCGGGAGTATATTCATGGATTCTTCAGCAATTTGGCAGAAATTTTGGATGATGGCTTTATACAGTATCTTCCTCATGTCGTACCCCTGGCTTTTTCTTCCTGCAATCTTGATGATGGAGCTGCAGTGGACATCGATGAGTCTGATGATGAAAACATTAATGGATTTGGTGGAGTTTCATCTGATGATGAAGCTCATGATGAGCCAAGAGTACGAAATATCAGCGTAAGAACAGGAGTTTTAGATGAAAAAGCAGCTGCGACTCAAGCTCTTGGCTTATTTGCACTACATACGAAGGCCTCATATGCAGTCTATTTGGAGGAATCATTTAAGATCCTAGTACGACACTCAGGGTATTTCCATGAAGATGTTCGGCTACAAGCAATCATTTCACTGAAACATATTTTAACAGCAGCACAGGCAGTTTACCAGAATCATAATGAAGGACAAGCAAGGGCTAAGGAAATCCTCGATACTGTGATGAATACTTATATCAAGACTATGACTGAAGATGATGACAAGGAAGTTGTTGCTCAAGCTTGTATGAGCCTTGCTGATATCATCAAAGATTATGGATATATGGTTGTTGAGCCTTATGTACCACGGGTTGTTGATGCTACTCTGGTACTGCTTCGGGAGGAATCAGCTTGTCAGCAGACAGAATCCGATGATGAAATTGATGACGATGATGTTGTACATGATGAAGAACTTATGGACGCAGTTTCTGACCTTCTTCCTGCTTTTGCAAAGTCAATGGGTCCTCATTTTGCACCTATTTTTGCTAAGCTATTTGAACCTTTAATGAAATTCGCGAGAGCTTCACGGCCATTGCAAGATCGGACAATGGTTGTTGCCTGCCTTGCTGAAGTTGCTCAAGACATGGGTGCTCCAATTGCTGGATATGTCGATAGGGTGATGCCCTTGGTAATCAAAGAACTAGCATCATCAGACGCCACCAATAGAAGGAATGCTGCTTTTTGTGTTGGAGAGTTCTGCAAAAATGGGGGCGAGGGGACGTTGAAATATTACGGTGATATATTGCGTGGACTTTACCCACTATTTGGGGAATCTGAGCCAGATGATGCTGTCAGGGATAATGCAGCTGGTGCAGTGGCAAGGATGATAATGGTGCACGCTGAATCTATCCCATTGAATCAGGTACTTCCTGTTTTCTTGAAGGCTCTTCCACTAAAAGAAGATCGTGAAGAGTCCATGGCGGTGTATAGTTGCGTCTGTACTCTTGTTTTATCATCGAATGCTCAGATCCTTTCTCTAGTCCCTGAATTGATTAATGTATTTGCTCAAGTTGTGGCGTCTCCGGTTGAAACTCCTGAAGTAAAAGCACAAATCGGAAGAGCTTTTTCTCACCTGATTTCCCTCTATGGCCATCAAATGCAGCCCCTTTTGAGTAACCTCTCCCCTGCTTATGCAAATGCCCTAGCTGCATTTGTCCCGAAAAGCTTATTCGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0017038 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061608 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT IBN_N Vac14_Fab1_bd

Protein Analysis

1052

Amino Acids

115.68

Weight (kDa)

4.89

Isoelectric Point (pI)

48.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	23 - 87	4.6e-15	Importin-beta N-terminal domain
TPR_IPO5	PF25780	93 - 251	4.5e-33	IPO5-like, TPR repeats
HEAT_GCN1	PF23271	271 - 447	9.8e-06	Stalled ribosome sensor GCN1-like, HEAT repeats
TOR1L1_N	PF24714	317 - 533	3e-09	TORTIFOLIA1 N-terminal
TPR_IMB1	PF25574	374 - 516	1.7e-10	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27640
fragaria_vesca	FvH4_5g29150
malus_domestica	MD08G1233900.v1.1 MD08G1234100.v1.1 MD15G1425400.v1.1
prunus_persica	Prupe.1G571100_v2.0.a1 Prupe.1G571100_v2.0.a1
pyrus_communis	pycom08g20240 pycom15g37710
rosa_chinensis	RchiOBHm_Chr7g0221821
rosa_laevigata	RLG00000002119
rosa_multiflora	Rmu_sc0005834.1_g000014
rosa_roxburghii	Rroxscaffold_3G00237670
rosa_rugosa	Rorug07G0203600
rosa_samantha	Rh7BG335300 Rh7CG362100 Rh7DG342400
rosa_wichuraiana	Rw7G029220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1441
AbsI	CCTCGAGG	1 cut(s)	1283
AccB7I	CCANNNNNTGG	2 cut(s)	2579, 2744
AccII	CGCG	2 cut(s)	525, 2495
AclWI	GGATC	8 cut(s)	412, 1124, 1137, 1160, 1370, 1557, 2011, 2941
AcoI	YGGCCR	6 cut(s)	90, 165, 347, 461, 2507, 3067
AcsI	RAATTY	8 cut(s)	566, 605, 684, 1359, 1522, 1673, 1725, 2489
AcuI	CTGAAG	4 cut(s)	1693, 2211, 2568, 3036
AcyI	GRCGYC	4 cut(s)	54, 146, 2634, 2996
AfaI	GTAC	9 cut(s)	123, 1767, 1896, 2025, 2283, 2310, 2379, 2844, 2920
AfiI	CCNNNNNNNGG	8 cut(s)	43, 64, 106, 722, 1385, 2579, 2646, 2744
AflIII	ACRYGT	1 cut(s)	794
AjiI	CACGTC	2 cut(s)	797, 1437
AjnI	CCWGG	1 cut(s)	1770
AjuI	GAANNNNNNNTTGG	4 cut(s)	1245, 1277, 1511, 1543
Alw21I	GWGCWC	4 cut(s)	273, 391, 2572, 2817
Alw26I	GTCTC	3 cut(s)	506, 1427, 3003
Alw44I	GTGCAC	2 cut(s)	387, 2813
AlwI	GGATC	8 cut(s)	412, 1124, 1137, 1160, 1370, 1557, 2011, 2941
AlwNI	CAGNNNCTG	2 cut(s)	13, 2408
Ama87I	CYCGRG	1 cut(s)	1283
AoxI	GGCC	9 cut(s)	90, 165, 204, 347, 461, 1401, 1980, 2507, 3067
ApaI	GGGCCC	1 cut(s)	1405
ApaLI	GTGCAC	2 cut(s)	387, 2813
ApoI	RAATTY	8 cut(s)	566, 605, 684, 1359, 1522, 1673, 1725, 2489
AseI	ATTAAT	2 cut(s)	1842, 2973
Asp700I	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
AspLEI	GCGC	2 cut(s)	118, 1157
AspS9I	GGNCC	7 cut(s)	73, 97, 204, 331, 1401, 1402, 2441
AsuC2I	CCSGG	2 cut(s)	58, 335
AsuHPI	GGTGA	6 cut(s)	295, 604, 1364, 2608, 2726, 3041
AsuNHI	GCTAGC	1 cut(s)	826
AvaI	CYCGRG	1 cut(s)	1283
AvaII	GGWCC	4 cut(s)	73, 97, 331, 2441
AxyI	CCTNAGG	2 cut(s)	102, 1170
BaeGI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BalI	TGGCCA	2 cut(s)	92, 3069
BamHI	GGATCC	1 cut(s)	1129
BanII	GRGCYC	1 cut(s)	1405
BauI	CACGAG	1 cut(s)	1560
Bbv12I	GWGCWC	4 cut(s)	273, 391, 2572, 2817
BccI	CCATC	6 cut(s)	572, 1378, 1612, 1730, 1793, 3078
BceAI	ACGGC	2 cut(s)	155, 2522
BciT130I	CCWGG	1 cut(s)	1772
BcnI	CCSGG	2 cut(s)	58, 335
BcoDI	GTCTC	3 cut(s)	506, 1427, 3003
BfaI	CTAG	6 cut(s)	827, 1614, 2021, 2621, 2957, 3122
BfmI	CTRYAG	5 cut(s)	503, 549, 957, 1470, 1806
BfoI	RGCGCY	1 cut(s)	1158
BlpI	GCTNAGC	1 cut(s)	2466
Bme1390I	CCNGG	3 cut(s)	58, 335, 1772
Bme18I	GGWCC	4 cut(s)	73, 97, 331, 2441
BmeT110I	CYCGRG	1 cut(s)	1283
BmgBI	CACGTC	2 cut(s)	797, 1437
BmgT120I	GGNCC	7 cut(s)	73, 97, 204, 331, 1401, 1402, 2441
BmiI	GGNNCC	8 cut(s)	99, 111, 333, 1131, 1402, 1403, 1623, 2442
BmrFI	CCNGG	3 cut(s)	58, 335, 1772
BmrI	ACTGGG	2 cut(s)	211, 1406
BmtI	GCTAGC	1 cut(s)	830
BmuI	ACTGGG	2 cut(s)	211, 1406
BpmI	CTGGAG	3 cut(s)	35, 360, 927
Bpu10I	CCTNAGC	1 cut(s)	2142
Bpu1102I	GCTNAGC	1 cut(s)	2466
BpuEI	CTTGAG	6 cut(s)	527, 754, 1932, 2202, 2541, 2970
BpuMI	CCSGG	2 cut(s)	58, 335
Bsa29I	ATCGAT	1 cut(s)	1818
BsaBI	GATNNNNATC	2 cut(s)	933, 1548
BsaHI	GRCGYC	4 cut(s)	54, 146, 2634, 2996
BsaJI	CCNNGG	5 cut(s)	93, 1770, 2285, 2604, 2895
BsaWI	WCCGGW	1 cut(s)	3001
BsaXI	ACNNNNNCTCC	6 cut(s)	114, 144, 280, 310, 1794, 1824
Bsc4I	CCNNNNNNNGG	8 cut(s)	43, 64, 106, 722, 1385, 2579, 2646, 2744
Bse118I	RCCGGY	2 cut(s)	280, 650
Bse1I	ACTGG	6 cut(s)	18, 199, 206, 294, 1133, 1401
Bse21I	CCTNAGG	2 cut(s)	102, 1170
Bse3DI	GCAATG	3 cut(s)	896, 1602, 2510
Bse8I	GATNNNNATC	2 cut(s)	933, 1548
BseBI	CCWGG	1 cut(s)	1772
BseCI	ATCGAT	1 cut(s)	1818
BseDI	CCNNGG	5 cut(s)	93, 1770, 2285, 2604, 2895
BseGI	GGATG	8 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2808
BseJI	GATNNNNATC	2 cut(s)	933, 1548
BseLI	CCNNNNNNNGG	8 cut(s)	43, 64, 106, 722, 1385, 2579, 2646, 2744
BseMI	GCAATG	3 cut(s)	896, 1602, 2510
BseMII	CTCAG	8 cut(s)	116, 170, 233, 1151, 1161, 2046, 2745, 2957
BseNI	ACTGG	6 cut(s)	18, 199, 206, 294, 1133, 1401
BseRI	GAGGAG	3 cut(s)	207, 301, 671
BseSI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BseYI	CCCAGC	1 cut(s)	614
BsgI	GTGCAG	2 cut(s)	522, 2811
Bsh1236I	CGCG	2 cut(s)	525, 2495
BshFI	GGCC	9 cut(s)	92, 167, 206, 349, 463, 1403, 1982, 2509, 3069
BshVI	ATCGAT	1 cut(s)	1818
BsiHKAI	GWGCWC	4 cut(s)	273, 391, 2572, 2817
BsiHKCI	CYCGRG	1 cut(s)	1283
BsiSI	CCGG	7 cut(s)	44, 58, 281, 335, 350, 651, 3002
BslFI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
BslI	CCNNNNNNNGG	8 cut(s)	43, 64, 106, 722, 1385, 2579, 2646, 2744
BsmAI	GTCTC	3 cut(s)	506, 1427, 3003
BsmBI	CGTCTC	2 cut(s)	1427, 3003
BsmFI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
BsmI	GAATGC	4 cut(s)	1282, 1481, 2656, 2944
BsnI	GGCC	9 cut(s)	92, 167, 206, 349, 463, 1403, 1982, 2509, 3069
BsoBI	CYCGRG	1 cut(s)	1283
Bsp120I	GGGCCC	1 cut(s)	1401
Bsp1286I	GDGCHC	6 cut(s)	273, 391, 896, 1405, 2572, 2817
Bsp1407I	TGTACA	1 cut(s)	2377
Bsp1720I	GCTNAGC	1 cut(s)	2466
Bsp19I	CCATGG	1 cut(s)	2895
Bsp68I	TCGCGA	1 cut(s)	2495
BspANI	GGCC	9 cut(s)	92, 167, 206, 349, 463, 1403, 1982, 2509, 3069
BspCNI	CTCAG	8 cut(s)	115, 171, 232, 1152, 1162, 2045, 2746, 2956
BspDI	ATCGAT	1 cut(s)	1818
BspFNI	CGCG	2 cut(s)	525, 2495
BspHI	TCATGA	2 cut(s)	574, 1879
BspLI	GGNNCC	8 cut(s)	99, 111, 333, 1131, 1402, 1403, 1623, 2442
BspMAI	CTGCAG	4 cut(s)	507, 961, 1474, 1810
BspOI	GCTAGC	1 cut(s)	830
BspPI	GGATC	8 cut(s)	412, 1124, 1137, 1160, 1370, 1557, 2011, 2941
BspQI	GCTCTTC	3 cut(s)	492, 2871, 3031
BsrDI	GCAATG	3 cut(s)	896, 1602, 2510
BsrFI	RCCGGY	2 cut(s)	280, 650
BsrGI	TGTACA	1 cut(s)	2377
BsrI	ACTGG	6 cut(s)	18, 199, 206, 294, 1133, 1401
BssAI	RCCGGY	2 cut(s)	280, 650
BssECI	CCNNGG	5 cut(s)	93, 1770, 2285, 2604, 2895
BssNI	GRCGYC	4 cut(s)	54, 146, 2634, 2996
BssSI	CACGAG	1 cut(s)	1560
BssT1I	CCWWGG	3 cut(s)	93, 2604, 2895
Bst2BI	CACGAG	1 cut(s)	1560
Bst2UI	CCWGG	1 cut(s)	1772
Bst4CI	ACNGT	4 cut(s)	275, 1749, 2161, 2714
Bst6I	CTCTTC	5 cut(s)	492, 723, 2871, 2883, 3031
BstACI	GRCGYC	4 cut(s)	54, 146, 2634, 2996
BstAPI	GCANNNNNTGC	4 cut(s)	326, 1199, 1469, 2789
BstAUI	TGTACA	1 cut(s)	2377
BstC8I	GCNNGC	8 cut(s)	66, 483, 503, 652, 828, 903, 2539, 2817
BstDSI	CCRYGG	2 cut(s)	2285, 2895
BstF5I	GGATG	8 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2808
BstFNI	CGCG	2 cut(s)	525, 2495
BstH2I	RGCGCY	1 cut(s)	1158
BstHHI	GCGC	2 cut(s)	118, 1157
BstMAI	GTCTC	3 cut(s)	506, 1427, 3003
BstNI	CCWGG	1 cut(s)	1772
BstNSI	RCATGY	2 cut(s)	1001, 1444
BstSCI	CCNGG	3 cut(s)	56, 333, 1770
BstSFI	CTRYAG	5 cut(s)	503, 549, 957, 1470, 1806
BstSLI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BstUI	CGCG	2 cut(s)	525, 2495
BstX2I	RGATCY	5 cut(s)	1129, 1165, 1549, 2016, 2946
BstYI	RGATCY	5 cut(s)	1129, 1165, 1549, 2016, 2946
Bsu15I	ATCGAT	1 cut(s)	1818
Bsu36I	CCTNAGG	2 cut(s)	102, 1170
BsuRI	GGCC	9 cut(s)	92, 167, 206, 349, 463, 1403, 1982, 2509, 3069
BsuTUI	ATCGAT	1 cut(s)	1818
BtgI	CCRYGG	2 cut(s)	2285, 2895
BtrI	CACGTC	2 cut(s)	797, 1437
BtsCI	GGATG	8 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2808
BtsI	GCAGTG	2 cut(s)	1815, 2799
BtsIMutI	CAGTG	9 cut(s)	11, 192, 199, 301, 1572, 1684, 1815, 2075, 2799
BtuMI	TCGCGA	1 cut(s)	2495
Cac8I	GCNNGC	8 cut(s)	66, 483, 503, 652, 828, 903, 2539, 2817
CaiI	CAGNNNCTG	2 cut(s)	13, 2408
CciI	TCATGA	2 cut(s)	574, 1879
CfoI	GCGC	2 cut(s)	118, 1157
Cfr10I	RCCGGY	2 cut(s)	280, 650
Cfr13I	GGNCC	7 cut(s)	73, 97, 204, 331, 1401, 1402, 2441
ClaI	ATCGAT	1 cut(s)	1818
CseI	GACGC	8 cut(s)	62, 154, 301, 514, 2408, 2642, 2902, 2985
Csp6I	GTAC	9 cut(s)	122, 1766, 1895, 2024, 2282, 2309, 2378, 2843, 2919
CspCI	CAANNNNNGTGG	2 cut(s)	1584, 1619
CviQI	GTAC	9 cut(s)	122, 1766, 1895, 2024, 2282, 2309, 2378, 2843, 2919
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	6 cut(s)	90, 165, 347, 461, 2507, 3067
Eam1104I	CTCTTC	5 cut(s)	492, 723, 2871, 2883, 3031
EarI	CTCTTC	5 cut(s)	492, 723, 2871, 2883, 3031
EciI	GGCGGA	2 cut(s)	83, 267
Eco130I	CCWWGG	3 cut(s)	93, 2604, 2895
Eco147I	AGGCCT	1 cut(s)	1982
Eco24I	GRGCYC	1 cut(s)	1405
Eco32I	GATATC	1 cut(s)	2242
Eco47I	GGWCC	4 cut(s)	73, 97, 331, 2441
Eco57I	CTGAAG	4 cut(s)	1693, 2211, 2568, 3036
Eco81I	CCTNAGG	2 cut(s)	102, 1170
Eco88I	CYCGRG	1 cut(s)	1283
EcoO109I	RGGNCCY	4 cut(s)	97, 1401, 1402, 2441
EcoRI	GAATTC	3 cut(s)	566, 605, 1673
EcoRII	CCWGG	1 cut(s)	1770
EcoRV	GATATC	1 cut(s)	2242
EcoT14I	CCWWGG	3 cut(s)	93, 2604, 2895
EcoT22I	ATGCAT	1 cut(s)	1294
EcoT38I	GRGCYC	1 cut(s)	1405
ErhI	CCWWGG	3 cut(s)	93, 2604, 2895
Esp3I	CGTCTC	2 cut(s)	1427, 3003
FaqI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
FauI	CCCGC	1 cut(s)	718
FauNDI	CATATG	1 cut(s)	1987
FokI	GGATG	8 cut(s)	521, 674, 857, 941, 1301, 1630, 1745, 2815
FriOI	GRGCYC	1 cut(s)	1405
FspBI	CTAG	6 cut(s)	827, 1614, 2021, 2621, 2957, 3122
GlaI	GCGC	2 cut(s)	117, 1156
GsaI	CCCAGC	1 cut(s)	618
GsuI	CTGGAG	3 cut(s)	35, 360, 927
HaeII	RGCGCY	1 cut(s)	1158
HaeIII	GGCC	9 cut(s)	92, 167, 206, 349, 463, 1403, 1982, 2509, 3069
HapII	CCGG	7 cut(s)	44, 58, 281, 335, 350, 651, 3002
HgaI	GACGC	8 cut(s)	62, 154, 301, 514, 2408, 2642, 2902, 2985
HhaI	GCGC	2 cut(s)	118, 1157
Hin1I	GRCGYC	4 cut(s)	54, 146, 2634, 2996
Hin6I	GCGC	2 cut(s)	116, 1155
HinP1I	GCGC	2 cut(s)	116, 1155
HindIII	AAGCTT	2 cut(s)	2220, 3142
HpaII	CCGG	7 cut(s)	44, 58, 281, 335, 350, 651, 3002
HphI	GGTGA	6 cut(s)	295, 604, 1364, 2608, 2726, 3041
Hpy166II	GTNNAC	8 cut(s)	227, 389, 1559, 1682, 1813, 2110, 2729, 2815
Hpy8I	GTNNAC	8 cut(s)	227, 389, 1559, 1682, 1813, 2110, 2729, 2815
Hpy99I	CGWCG	2 cut(s)	56, 317
HpyAV	CCTTC	6 cut(s)	1300, 1972, 2120, 2423, 2640, 2854
HpyCH4III	ACNGT	4 cut(s)	275, 1749, 2161, 2714
HpyCH4IV	ACGT	4 cut(s)	153, 796, 1436, 2699
HpySE526I	ACGT	4 cut(s)	153, 796, 1436, 2699
Hsp92I	GRCGYC	4 cut(s)	54, 146, 2634, 2996
HspAI	GCGC	2 cut(s)	116, 1155
KroI	GCCGGC	1 cut(s)	650
KroNI	GCCGGC	1 cut(s)	652
LguI	GCTCTTC	3 cut(s)	492, 2871, 3031
LmnI	GCTCC	6 cut(s)	16, 268, 946, 1219, 1802, 2575
MaeI	CTAG	6 cut(s)	827, 1614, 2021, 2621, 2957, 3122
MaeII	ACGT	4 cut(s)	153, 796, 1436, 2699
MaeIII	GTNAC	4 cut(s)	9, 1072, 1340, 3092
MfeI	CAATTG	3 cut(s)	161, 1449, 2574
MflI	RGATCY	5 cut(s)	1129, 1165, 1549, 2016, 2946
MhlI	GDGCHC	6 cut(s)	273, 391, 896, 1405, 2572, 2817
MlsI	TGGCCA	2 cut(s)	92, 3069
MluNI	TGGCCA	2 cut(s)	92, 3069
MlyI	GAGTC	3 cut(s)	1832, 1939, 2900
MmeI	TCCRAC	3 cut(s)	1107, 1564, 2647
Mox20I	TGGCCA	2 cut(s)	92, 3069
Mph1103I	ATGCAT	1 cut(s)	1294
MroNI	GCCGGC	1 cut(s)	650
MroXI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
MscI	TGGCCA	2 cut(s)	92, 3069
MseI	TTAA	9 cut(s)	237, 623, 735, 1031, 1842, 2013, 2090, 2483, 2973
MslI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
Msp20I	TGGCCA	2 cut(s)	92, 3069
MspA1I	CMGCKG	2 cut(s)	1940, 2786
MspI	CCGG	7 cut(s)	44, 58, 281, 335, 350, 651, 3002
MspR9I	CCNGG	3 cut(s)	58, 335, 1772
MunI	CAATTG	3 cut(s)	161, 1449, 2574
Mva1269I	GAATGC	4 cut(s)	1282, 1481, 2656, 2944
MvaI	CCWGG	1 cut(s)	1772
MvnI	CGCG	2 cut(s)	525, 2495
NaeI	GCCGGC	1 cut(s)	652
NciI	CCSGG	2 cut(s)	58, 335
NcoI	CCATGG	1 cut(s)	2895
NdeI	CATATG	1 cut(s)	1987
NgoMIV	GCCGGC	1 cut(s)	650
NheI	GCTAGC	1 cut(s)	826
NlaIV	GGNNCC	8 cut(s)	99, 111, 333, 1131, 1402, 1403, 1623, 2442
NmuCI	GTSAC	2 cut(s)	9, 1340
NruI	TCGCGA	1 cut(s)	2495
NsiI	ATGCAT	1 cut(s)	1294
NspI	RCATGY	2 cut(s)	1001, 1444
PaeR7I	CTCGAG	1 cut(s)	1283
PagI	TCATGA	2 cut(s)	574, 1879
PceI	AGGCCT	1 cut(s)	1982
PciSI	GCTCTTC	3 cut(s)	492, 2871, 3031
PctI	GAATGC	4 cut(s)	1282, 1481, 2656, 2944
PdiI	GCCGGC	1 cut(s)	652
PdmI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
PflMI	CCANNNNNTGG	2 cut(s)	2579, 2744
PleI	GAGTC	3 cut(s)	1831, 1939, 2899
PpsI	GAGTC	3 cut(s)	1831, 1939, 2899
PpuMI	RGGWCCY	2 cut(s)	97, 2441
PshBI	ATTAAT	2 cut(s)	1842, 2973
Psp5II	RGGWCCY	2 cut(s)	97, 2441
Psp6I	CCWGG	1 cut(s)	1770
PspFI	CCCAGC	1 cut(s)	614
PspGI	CCWGG	1 cut(s)	1770
PspN4I	GGNNCC	8 cut(s)	99, 111, 333, 1131, 1402, 1403, 1623, 2442
PspOMI	GGGCCC	1 cut(s)	1401
PspPI	GGNCC	7 cut(s)	73, 97, 204, 331, 1401, 1402, 2441
PspPPI	RGGWCCY	2 cut(s)	97, 2441
PspXI	VCTCGAGB	1 cut(s)	1283
PsrI	GAACNNNNNNTAC	2 cut(s)	1614, 1646
PstI	CTGCAG	4 cut(s)	507, 961, 1474, 1810
PstNI	CAGNNNCTG	2 cut(s)	13, 2408
PsuI	RGATCY	5 cut(s)	1129, 1165, 1549, 2016, 2946
PvuII	CAGCTG	2 cut(s)	1940, 2786
RruI	TCGCGA	1 cut(s)	2495
RsaI	GTAC	9 cut(s)	123, 1767, 1896, 2025, 2283, 2310, 2379, 2844, 2920
RsaNI	GTAC	9 cut(s)	122, 1766, 1895, 2024, 2282, 2309, 2378, 2843, 2919
RseI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
SapI	GCTCTTC	3 cut(s)	492, 2871, 3031
SaqAI	TTAA	9 cut(s)	237, 623, 735, 1031, 1842, 2013, 2090, 2483, 2973
Sau96I	GGNCC	7 cut(s)	73, 97, 204, 331, 1401, 1402, 2441
SbfI	CCTGCAGG	1 cut(s)	507
SchI	GAGTC	3 cut(s)	1832, 1939, 2900
ScrFI	CCNGG	3 cut(s)	58, 335, 1772
SdaI	CCTGCAGG	1 cut(s)	507
SduI	GDGCHC	6 cut(s)	273, 391, 896, 1405, 2572, 2817
SfcI	CTRYAG	5 cut(s)	503, 549, 957, 1470, 1806
Sfr274I	CTCGAG	1 cut(s)	1283
SgrAI	CRCCGGYG	1 cut(s)	280
SinI	GGWCC	4 cut(s)	73, 97, 331, 2441
SlaI	CTCGAG	1 cut(s)	1283
SmiMI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
SmlI	CTYRAG	7 cut(s)	542, 769, 1283, 1947, 2217, 2556, 2985
SmoI	CTYRAG	7 cut(s)	542, 769, 1283, 1947, 2217, 2556, 2985
Sse8387I	CCTGCAGG	1 cut(s)	507
SseBI	AGGCCT	1 cut(s)	1982
SspI	AATATT	1 cut(s)	2708
SspMI	CTAG	6 cut(s)	827, 1614, 2021, 2621, 2957, 3122
StuI	AGGCCT	1 cut(s)	1982
StyD4I	CCNGG	3 cut(s)	56, 333, 1770
StyI	CCWWGG	3 cut(s)	93, 2604, 2895
TaaI	ACNGT	4 cut(s)	275, 1749, 2161, 2714
TaiI	ACGT	4 cut(s)	156, 799, 1439, 2702
TaqI	TCGA	6 cut(s)	601, 1284, 1818, 2154, 2589, 2936
TatI	WGTACW	2 cut(s)	2377, 2918
TauI	GCSGC	3 cut(s)	64, 160, 170
Tru1I	TTAA	9 cut(s)	237, 623, 735, 1031, 1842, 2013, 2090, 2483, 2973
Tru9I	TTAA	9 cut(s)	237, 623, 735, 1031, 1842, 2013, 2090, 2483, 2973
TscAI	CASTG	9 cut(s)	18, 199, 206, 301, 1579, 1684, 1815, 2082, 2799
TseFI	GTSAC	2 cut(s)	9, 1340
Tsp45I	GTSAC	2 cut(s)	9, 1340
TspGWI	ACGGA	1 cut(s)	1368
TspRI	CASTG	9 cut(s)	18, 199, 206, 301, 1579, 1684, 1815, 2082, 2799
Van91I	CCANNNNNTGG	2 cut(s)	2579, 2744
VneI	GTGCAC	2 cut(s)	387, 2813
VpaK11BI	GGWCC	4 cut(s)	73, 97, 331, 2441
VspI	ATTAAT	2 cut(s)	1842, 2973
XapI	RAATTY	8 cut(s)	566, 605, 684, 1359, 1522, 1673, 1725, 2489
XceI	RCATGY	2 cut(s)	1001, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	301
XhoI	CTCGAG	1 cut(s)	1283
XmnI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
XspI	CTAG	6 cut(s)	827, 1614, 2021, 2621, 2957, 3122
Zsp2I	ATGCAT	1 cut(s)	1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.