Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0013167

Physical Location & Seq

Genomic Coordinates Reverse (-)

20107104 .. 20112008

Gene Structure 4905 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g20240.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGTCGCAGTCACTGGAGCTCTTGCTGATACAATTCCTGATGCCGGACAACGACGCCCGGCGGCAGGCGGAGGACCAGATAAAGCGCTTGGCCAAGGACCCTCAGGTGGTTCCCGCGCTCGTACAGCACCTCCGCACCGCCAAGACGCCGAACGTGAGGCAATTGGCGGCCGTGCTTCTGAGGAAGAAGATCACCGGACACTGGGCCAAGCTCTCACCTCAAATTAAACACCTCGTTAAGCAGTCCCTCATTGAGAGCATCACAATGGAGCACAGTCCACCGGTGAGGCGAGCCAGTGCCAATGTGGTTAGTGTCGTCGCAAAATATGCAGTCCCGGCTGGAGAATGGCCGGATTTGTTGCCCTTTCTGTTTCAATGTAGTCAGAGTGCACAGGAAGAACATAGAGAGGTGGCATTGATCCTGTTCAGCTCTTTGACTGAAACAATAGGGAATACGTTTCGGCCACATTTCGCAGATTTGCAAGCTCTTCTTCTGAAATGCCTCCAGGATGAGACAAGCAACCGCGTCAGAGTTGCTGCGCTCAAGGCCGTAGGGTCTTTTCTGGAATTCACTCATGATGGGGTTGAAGTGGTGAAGTTTCGGGAATTCATACCTAGCATCTTAAATGTATCAAGACAATGCCTTGCAGCTGGCGAGGAGGATGTTGCTATTATTGCTTTTGAAATTTTTGATGAGTTGATTGAATCTCCTGCACCTCTTCTTGGTGAATCTGTTAAATCCATTGTGCAATTCTCTCTTGAAGTTTGTTCAACTCAAAGTTTAGAATCTAATACACGTCATCAGGCAATTCAGATAGTTTCATGGCTAGCAAAGTATAAATCCAATTCCCTCAAAAAGCATAAGCTGGTCATCCCTATTCTGCAAGTTATGTGCCCATTGCTTGCAGAATCAAATGATGAAGATAAGGATGATGATCTTGCTCCAGATCGAGCTGCTGCAGAAGTTATTGATACTATGGCTTTGAACATACCAAAGCATGTTTTCCACCCTGTCTTTGAATTTTCTTCTTTAAGCAGTCAAAACGCAAATCCAAAATATCGGGAAGCATCTGTTACCGCTTTAGGTGTCATTTCAGAGGGTTGTCTGGAGATGATAAAAGATAAGTTGGATCCAGTTCTTCATATTGTCTTAGGCGCTCTGAGCGATCCTGAGGAAATGGTTAGGGGGGCTGCATCATTTGCATTGGGTCAGTTTGCAGAGCATTTGCAGCCGGAAATTGTTTCCCACTATCAAAGTGTACTTCCCTGCATTTTGAATGCCCTTGAGGATACATCTGATGAAGTGAAGGAGAAGTCATATTATGCTTTGGCAGCATTTTGTGACAACATGGGTGAGGAAATTCTTCCATTCCTAGATCCGTTGATGGGAAAACTACTGGGGGCCCTCCATAATAGCCCTCGTAATTTGCAGGAGACATGCATGTCTGCGATTGGTTCGGTTGCATCTGCGGCAGAACAGGCATTTGTTCCGTATGCTGAAAGGGTTTTGGAGTTGATGAAAAGTTTCTTGGTGCTTAGTAATGATGAGGATCGATTCATTCACGAGCAAGAGCTACTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0017038 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061608 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT IBN_N Vac14_Fab1_bd

Protein Analysis

528

Amino Acids

58.5

Weight (kDa)

5.2

Isoelectric Point (pI)

52.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27640
fragaria_vesca	FvH4_5g29150
malus_domestica	MD08G1233900.v1.1 MD08G1234100.v1.1 MD15G1425400.v1.1
prunus_persica	Prupe.1G571100_v2.0.a1 Prupe.1G571100_v2.0.a1
pyrus_communis	pycom08g20240 pycom15g37710
rosa_chinensis	RchiOBHm_Chr7g0221821
rosa_laevigata	RLG00000002119
rosa_multiflora	Rmu_sc0005834.1_g000014
rosa_roxburghii	Rroxscaffold_3G00237670
rosa_rugosa	Rorug07G0203600
rosa_samantha	Rh7BG335300 Rh7CG362100 Rh7DG342400
rosa_wichuraiana	Rw7G029220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1441
AccII	CGCG	2 cut(s)	116, 525
AciI	CCGC	9 cut(s)	61, 68, 114, 133, 138, 167, 523, 1077, 1469
AclWI	GGATC	6 cut(s)	412, 1124, 1137, 1160, 1370, 1557
AcoI	YGGCCR	4 cut(s)	90, 168, 347, 461
AcsI	RAATTY	5 cut(s)	566, 605, 684, 1019, 1359
AcyI	GRCGYC	2 cut(s)	54, 146
AfaI	GTAC	2 cut(s)	123, 1260
AfeI	AGCGCT	1 cut(s)	86
AfiI	CCNNNNNNNGG	6 cut(s)	43, 64, 106, 201, 722, 1385
AflIII	ACRYGT	1 cut(s)	794
AgeI	ACCGGT	1 cut(s)	280
AgsI	TTSAA	9 cut(s)	374, 587, 683, 704, 761, 771, 985, 1019, 1276
AjiI	CACGTC	1 cut(s)	797
AjnI	CCWGG	1 cut(s)	504
AjuI	GAANNNNNNNTTGG	2 cut(s)	1511, 1543
AluBI	AGCT	8 cut(s)	19, 211, 429, 485, 650, 865, 953, 1573
AluI	AGCT	8 cut(s)	19, 211, 429, 485, 650, 865, 953, 1573
Alw21I	GWGCWC	3 cut(s)	21, 273, 391
Alw26I	GTCTC	2 cut(s)	506, 1427
Alw44I	GTGCAC	1 cut(s)	387
AlwI	GGATC	6 cut(s)	412, 1124, 1137, 1160, 1370, 1557
AlwNI	CAGNNNCTG	1 cut(s)	13
Aor51HI	AGCGCT	1 cut(s)	86
AoxI	GGCC	7 cut(s)	90, 168, 204, 347, 461, 546, 1401
ApaI	GGGCCC	1 cut(s)	1405
ApaLI	GTGCAC	1 cut(s)	387
ApeKI	GCWGC	7 cut(s)	536, 647, 953, 956, 1190, 1228, 1331
ApoI	RAATTY	5 cut(s)	566, 605, 684, 1019, 1359
AsiGI	ACCGGT	1 cut(s)	280
Asp700I	GAANNNNTTC	2 cut(s)	1362, 1523
AspLEI	GCGC	4 cut(s)	87, 118, 541, 1157
AspS9I	GGNCC	5 cut(s)	73, 97, 204, 1401, 1402
AsuC2I	CCSGG	2 cut(s)	58, 335
AsuHPI	GGTGA	6 cut(s)	184, 207, 295, 604, 737, 1364
AsuNHI	GCTAGC	1 cut(s)	826
AvaII	GGWCC	2 cut(s)	73, 97
AxyI	CCTNAGG	2 cut(s)	102, 1170
BaeGI	GKGCMC	3 cut(s)	391, 896, 1405
BalI	TGGCCA	1 cut(s)	92
BamHI	GGATCC	1 cut(s)	1129
BanII	GRGCYC	2 cut(s)	21, 1405
BauI	CACGAG	1 cut(s)	1562
Bbv12I	GWGCWC	3 cut(s)	21, 273, 391
BbvI	GCAGC	7 cut(s)	523, 659, 940, 943, 1177, 1240, 1343
BccI	CCATC	2 cut(s)	572, 1378
BceAI	ACGGC	2 cut(s)	155, 533
BciT130I	CCWGG	1 cut(s)	506
BciVI	GTATCC	1 cut(s)	1282
BcnI	CCSGG	2 cut(s)	58, 335
BcoDI	GTCTC	2 cut(s)	506, 1427
BfaI	CTAG	3 cut(s)	615, 827, 1373
BfmI	CTRYAG	1 cut(s)	957
BfoI	RGCGCY	2 cut(s)	88, 1158
BfuI	GTATCC	1 cut(s)	1282
Bme1390I	CCNGG	3 cut(s)	58, 335, 506
Bme18I	GGWCC	2 cut(s)	73, 97
BmgBI	CACGTC	1 cut(s)	797
BmgT120I	GGNCC	5 cut(s)	73, 97, 204, 1401, 1402
BmiI	GGNNCC	5 cut(s)	99, 111, 1131, 1402, 1403
BmrFI	CCNGG	3 cut(s)	58, 335, 506
BmrI	ACTGGG	2 cut(s)	211, 1406
BmsI	GCATC	6 cut(s)	30, 267, 627, 1076, 1202, 1472
BmtI	GCTAGC	1 cut(s)	830
BmuI	ACTGGG	2 cut(s)	211, 1406
BpmI	CTGGAG	5 cut(s)	35, 360, 488, 927, 1127
BpuEI	CTTGAG	2 cut(s)	527, 1304
BpuMI	CCSGG	2 cut(s)	58, 335
Bsa29I	ATCGAT	1 cut(s)	1552
BsaBI	GATNNNNATC	2 cut(s)	933, 1548
BsaHI	GRCGYC	2 cut(s)	54, 146
BsaJI	CCNNGG	1 cut(s)	93
BsaWI	WCCGGW	2 cut(s)	194, 280
BsaXI	ACNNNNNCTCC	2 cut(s)	114, 144
Bsc4I	CCNNNNNNNGG	6 cut(s)	43, 64, 106, 201, 722, 1385
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	5 cut(s)	18, 206, 294, 1133, 1401
Bse21I	CCTNAGG	2 cut(s)	102, 1170
Bse3DI	GCAATG	1 cut(s)	896
Bse8I	GATNNNNATC	2 cut(s)	933, 1548
BseBI	CCWGG	1 cut(s)	506
BseCI	ATCGAT	1 cut(s)	1552
BseDI	CCNNGG	1 cut(s)	93
BseGI	GGATG	4 cut(s)	514, 667, 870, 934
BseJI	GATNNNNATC	2 cut(s)	933, 1548
BseLI	CCNNNNNNNGG	6 cut(s)	43, 64, 106, 201, 722, 1385
BseMI	GCAATG	1 cut(s)	896
BseMII	CTCAG	4 cut(s)	116, 170, 1151, 1161
BseNI	ACTGG	5 cut(s)	18, 206, 294, 1133, 1401
BseRI	GAGGAG	1 cut(s)	671
BseSI	GKGCMC	3 cut(s)	391, 896, 1405
BseX3I	CGGCCG	1 cut(s)	168
BseXI	GCAGC	7 cut(s)	523, 659, 940, 943, 1177, 1240, 1343
BsgI	GTGCAG	1 cut(s)	696
Bsh1236I	CGCG	2 cut(s)	116, 525
Bsh1285I	CGRYCG	1 cut(s)	171
BshFI	GGCC	7 cut(s)	92, 170, 206, 349, 463, 548, 1403
BshTI	ACCGGT	1 cut(s)	280
BshVI	ATCGAT	1 cut(s)	1552
BsiEI	CGRYCG	1 cut(s)	171
BsiHKAI	GWGCWC	3 cut(s)	21, 273, 391
BsiSI	CCGG	7 cut(s)	44, 58, 195, 281, 335, 350, 1232
BslFI	GGGAC	2 cut(s)	229, 317
BslI	CCNNNNNNNGG	6 cut(s)	43, 64, 106, 201, 722, 1385
BsmAI	GTCTC	2 cut(s)	506, 1427
BsmFI	GGGAC	2 cut(s)	229, 317
BsmI	GAATGC	1 cut(s)	1282
BsnI	GGCC	7 cut(s)	92, 170, 206, 349, 463, 548, 1403
Bsp120I	GGGCCC	1 cut(s)	1401
Bsp1286I	GDGCHC	5 cut(s)	21, 273, 391, 896, 1405
Bsp143I	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
BspACI	CCGC	9 cut(s)	61, 68, 114, 133, 138, 167, 523, 1077, 1469
BspANI	GGCC	7 cut(s)	92, 170, 206, 349, 463, 548, 1403
BspCNI	CTCAG	4 cut(s)	115, 171, 1152, 1162
BspDI	ATCGAT	1 cut(s)	1552
BspFNI	CGCG	2 cut(s)	116, 525
BspHI	TCATGA	1 cut(s)	574
BspLI	GGNNCC	5 cut(s)	99, 111, 1131, 1402, 1403
BspMAI	CTGCAG	1 cut(s)	961
BspOI	GCTAGC	1 cut(s)	830
BspPI	GGATC	6 cut(s)	412, 1124, 1137, 1160, 1370, 1557
BspQI	GCTCTTC	1 cut(s)	492
BsrDI	GCAATG	1 cut(s)	896
BsrFI	RCCGGY	1 cut(s)	280
BsrI	ACTGG	5 cut(s)	18, 206, 294, 1133, 1401
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	1 cut(s)	93
BssMI	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
BssNI	GRCGYC	2 cut(s)	54, 146
BssSI	CACGAG	1 cut(s)	1562
BssT1I	CCWWGG	1 cut(s)	93
Bst2BI	CACGAG	1 cut(s)	1562
Bst2UI	CCWGG	1 cut(s)	506
Bst4CI	ACNGT	1 cut(s)	275
Bst6I	CTCTTC	2 cut(s)	492, 723
BstACI	GRCGYC	2 cut(s)	54, 146
BstAPI	GCANNNNNTGC	2 cut(s)	326, 1199
BstC8I	GCNNGC	6 cut(s)	66, 291, 483, 652, 828, 903
BstDEI	CTNAG	6 cut(s)	102, 179, 1150, 1160, 1170, 1535
BstF5I	GGATG	4 cut(s)	514, 667, 870, 934
BstFNI	CGCG	2 cut(s)	116, 525
BstH2I	RGCGCY	2 cut(s)	88, 1158
BstHHI	GCGC	4 cut(s)	87, 118, 541, 1157
BstKTI	GATC	8 cut(s)	192, 420, 937, 949, 1132, 1168, 1378, 1552
BstMAI	GTCTC	2 cut(s)	506, 1427
BstMBI	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
BstMCI	CGRYCG	1 cut(s)	171
BstNI	CCWGG	1 cut(s)	506
BstNSI	RCATGY	3 cut(s)	1001, 1440, 1444
BstSCI	CCNGG	3 cut(s)	56, 333, 504
BstSFI	CTRYAG	1 cut(s)	957
BstSLI	GKGCMC	3 cut(s)	391, 896, 1405
BstUI	CGCG	2 cut(s)	116, 525
BstV1I	GCAGC	7 cut(s)	523, 659, 940, 943, 1177, 1240, 1343
BstX2I	RGATCY	2 cut(s)	1129, 1375
BstYI	RGATCY	2 cut(s)	1129, 1375
BstZI	CGGCCG	1 cut(s)	168
Bsu15I	ATCGAT	1 cut(s)	1552
Bsu36I	CCTNAGG	2 cut(s)	102, 1170
BsuI	GTATCC	1 cut(s)	1282
BsuRI	GGCC	7 cut(s)	92, 170, 206, 349, 463, 548, 1403
BsuTUI	ATCGAT	1 cut(s)	1552
BtrI	CACGTC	1 cut(s)	797
BtsCI	GGATG	4 cut(s)	514, 667, 870, 934
BtsIMutI	CAGTG	3 cut(s)	11, 199, 301
Cac8I	GCNNGC	6 cut(s)	66, 291, 483, 652, 828, 903
CaiI	CAGNNNCTG	1 cut(s)	13
CciI	TCATGA	1 cut(s)	574
CfoI	GCGC	4 cut(s)	87, 118, 541, 1157
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	5 cut(s)	73, 97, 204, 1401, 1402
ClaI	ATCGAT	1 cut(s)	1552
CseI	GACGC	3 cut(s)	62, 154, 514
Csp6I	GTAC	2 cut(s)	122, 1259
CspAI	ACCGGT	1 cut(s)	280
CviAII	CATG	6 cut(s)	575, 822, 998, 1348, 1437, 1441
CviQI	GTAC	2 cut(s)	122, 1259
DdeI	CTNAG	6 cut(s)	102, 179, 1150, 1160, 1170, 1535
DpnI	GATC	8 cut(s)	191, 419, 936, 948, 1131, 1167, 1377, 1551
DpnII	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	4 cut(s)	90, 168, 347, 461
EagI	CGGCCG	1 cut(s)	168
Eam1104I	CTCTTC	2 cut(s)	492, 723
EarI	CTCTTC	2 cut(s)	492, 723
EciI	GGCGGA	1 cut(s)	83
Ecl136II	GAGCTC	1 cut(s)	19
EclXI	CGGCCG	1 cut(s)	168
Eco130I	CCWWGG	1 cut(s)	93
Eco24I	GRGCYC	2 cut(s)	21, 1405
Eco47I	GGWCC	2 cut(s)	73, 97
Eco47III	AGCGCT	1 cut(s)	86
Eco52I	CGGCCG	1 cut(s)	168
Eco53kI	GAGCTC	1 cut(s)	19
Eco81I	CCTNAGG	2 cut(s)	102, 1170
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	3 cut(s)	97, 1401, 1402
EcoRI	GAATTC	2 cut(s)	566, 605
EcoRII	CCWGG	1 cut(s)	504
EcoT14I	CCWWGG	1 cut(s)	93
EcoT22I	ATGCAT	1 cut(s)	1442
EcoT38I	GRGCYC	2 cut(s)	21, 1405
ErhI	CCWWGG	1 cut(s)	93
FaeI	CATG	6 cut(s)	578, 825, 1001, 1351, 1440, 1444
FalI	AAGNNNNNCTT	2 cut(s)	474, 506
FaqI	GGGAC	2 cut(s)	229, 317
FatI	CATG	6 cut(s)	574, 821, 997, 1347, 1436, 1440
FauI	CCCGC	1 cut(s)	121
FokI	GGATG	4 cut(s)	521, 674, 857, 941
FriOI	GRGCYC	2 cut(s)	21, 1405
FspBI	CTAG	3 cut(s)	615, 827, 1373
GlaI	GCGC	4 cut(s)	86, 117, 540, 1156
GsuI	CTGGAG	5 cut(s)	35, 360, 488, 927, 1127
HaeII	RGCGCY	2 cut(s)	88, 1158
HaeIII	GGCC	7 cut(s)	92, 170, 206, 349, 463, 548, 1403
HapII	CCGG	7 cut(s)	44, 58, 195, 281, 335, 350, 1232
HgaI	GACGC	3 cut(s)	62, 154, 514
HhaI	GCGC	4 cut(s)	87, 118, 541, 1157
Hin1I	GRCGYC	2 cut(s)	54, 146
Hin1II	CATG	6 cut(s)	578, 825, 1001, 1351, 1440, 1444
Hin6I	GCGC	4 cut(s)	85, 116, 539, 1155
HinP1I	GCGC	4 cut(s)	85, 116, 539, 1155
HinfI	GANTC	5 cut(s)	704, 728, 785, 908, 1554
HpaII	CCGG	7 cut(s)	44, 58, 195, 281, 335, 350, 1232
HphI	GGTGA	6 cut(s)	184, 207, 295, 604, 737, 1364
Hpy166II	GTNNAC	3 cut(s)	278, 389, 1259
Hpy188I	TCNGA	8 cut(s)	180, 384, 495, 530, 813, 1096, 1161, 1297
Hpy8I	GTNNAC	3 cut(s)	278, 389, 1259
Hpy99I	CGWCG	2 cut(s)	56, 320
HpyAV	CCTTC	1 cut(s)	1300
HpyCH4III	ACNGT	1 cut(s)	275
HpyCH4IV	ACGT	3 cut(s)	153, 455, 796
HpyF3I	CTNAG	6 cut(s)	102, 179, 1150, 1160, 1170, 1535
HpySE526I	ACGT	3 cut(s)	153, 455, 796
Hsp92I	GRCGYC	2 cut(s)	54, 146
Hsp92II	CATG	6 cut(s)	578, 825, 1001, 1351, 1440, 1444
HspAI	GCGC	4 cut(s)	85, 116, 539, 1155
Kzo9I	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
LguI	GCTCTTC	1 cut(s)	492
LmnI	GCTCC	3 cut(s)	16, 268, 946
Lsp1109I	GCAGC	7 cut(s)	523, 659, 940, 943, 1177, 1240, 1343
LweI	GCATC	6 cut(s)	30, 267, 627, 1076, 1202, 1472
MaeI	CTAG	3 cut(s)	615, 827, 1373
MaeII	ACGT	3 cut(s)	153, 455, 796
MaeIII	GTNAC	3 cut(s)	9, 1072, 1340
MalI	GATC	8 cut(s)	191, 419, 936, 948, 1131, 1167, 1377, 1551
MboI	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
MfeI	CAATTG	1 cut(s)	161
MflI	RGATCY	2 cut(s)	1129, 1375
MhlI	GDGCHC	5 cut(s)	21, 273, 391, 896, 1405
MlsI	TGGCCA	1 cut(s)	92
MluNI	TGGCCA	1 cut(s)	92
MmeI	TCCRAC	1 cut(s)	1107
Mox20I	TGGCCA	1 cut(s)	92
Mph1103I	ATGCAT	1 cut(s)	1442
MroXI	GAANNNNTTC	2 cut(s)	1362, 1523
MscI	TGGCCA	1 cut(s)	92
MseI	TTAA	5 cut(s)	225, 237, 623, 735, 1031
MslI	CAYNNNNRTG	1 cut(s)	263
Msp20I	TGGCCA	1 cut(s)	92
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	7 cut(s)	44, 58, 195, 281, 335, 350, 1232
MspR9I	CCNGG	3 cut(s)	58, 335, 506
MunI	CAATTG	1 cut(s)	161
Mva1269I	GAATGC	1 cut(s)	1282
MvaI	CCWGG	1 cut(s)	506
MvnI	CGCG	2 cut(s)	116, 525
NciI	CCSGG	2 cut(s)	58, 335
NdeII	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
NheI	GCTAGC	1 cut(s)	826
NlaIII	CATG	6 cut(s)	578, 825, 1001, 1351, 1440, 1444
NlaIV	GGNNCC	5 cut(s)	99, 111, 1131, 1402, 1403
NmuCI	GTSAC	2 cut(s)	9, 1340
NsiI	ATGCAT	1 cut(s)	1442
NspI	RCATGY	3 cut(s)	1001, 1440, 1444
PagI	TCATGA	1 cut(s)	574
PciSI	GCTCTTC	1 cut(s)	492
PctI	GAATGC	1 cut(s)	1282
PdmI	GAANNNNTTC	2 cut(s)	1362, 1523
PfeI	GAWTC	5 cut(s)	704, 728, 785, 908, 1554
PfoI	TCCNGGA	1 cut(s)	504
PinAI	ACCGGT	1 cut(s)	280
PpuMI	RGGWCCY	1 cut(s)	97
Psp124BI	GAGCTC	1 cut(s)	21
Psp5II	RGGWCCY	1 cut(s)	97
Psp6I	CCWGG	1 cut(s)	504
PspGI	CCWGG	1 cut(s)	504
PspN4I	GGNNCC	5 cut(s)	99, 111, 1131, 1402, 1403
PspOMI	GGGCCC	1 cut(s)	1401
PspPI	GGNCC	5 cut(s)	73, 97, 204, 1401, 1402
PspPPI	RGGWCCY	1 cut(s)	97
PstI	CTGCAG	1 cut(s)	961
PstNI	CAGNNNCTG	1 cut(s)	13
PsuI	RGATCY	2 cut(s)	1129, 1375
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	2 cut(s)	123, 1260
RsaNI	GTAC	2 cut(s)	122, 1259
RseI	CAYNNNNRTG	1 cut(s)	263
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	1 cut(s)	492
SaqAI	TTAA	5 cut(s)	225, 237, 623, 735, 1031
Sau3AI	GATC	8 cut(s)	189, 417, 934, 946, 1129, 1165, 1375, 1549
Sau96I	GGNCC	5 cut(s)	73, 97, 204, 1401, 1402
ScrFI	CCNGG	3 cut(s)	58, 335, 506
SduI	GDGCHC	5 cut(s)	21, 273, 391, 896, 1405
SfaNI	GCATC	6 cut(s)	30, 267, 627, 1076, 1202, 1472
SfcI	CTRYAG	1 cut(s)	957
SgrAI	CRCCGGYG	1 cut(s)	280
SinI	GGWCC	2 cut(s)	73, 97
SmiMI	CAYNNNNRTG	1 cut(s)	263
SmlI	CTYRAG	2 cut(s)	542, 1283
SmoI	CTYRAG	2 cut(s)	542, 1283
SsiI	CCGC	9 cut(s)	61, 68, 114, 133, 138, 167, 523, 1077, 1469
SspMI	CTAG	3 cut(s)	615, 827, 1373
SstI	GAGCTC	1 cut(s)	21
StyD4I	CCNGG	3 cut(s)	56, 333, 504
StyI	CCWWGG	1 cut(s)	93
TaaI	ACNGT	1 cut(s)	275
TaiI	ACGT	3 cut(s)	156, 458, 799
TaqI	TCGA	2 cut(s)	949, 1552
TatI	WGTACW	1 cut(s)	1258
TauI	GCSGC	3 cut(s)	64, 170, 1472
TfiI	GAWTC	5 cut(s)	704, 728, 785, 908, 1554
Tru1I	TTAA	5 cut(s)	225, 237, 623, 735, 1031
Tru9I	TTAA	5 cut(s)	225, 237, 623, 735, 1031
TscAI	CASTG	3 cut(s)	18, 206, 301
TseFI	GTSAC	2 cut(s)	9, 1340
TseI	GCWGC	7 cut(s)	536, 647, 953, 956, 1190, 1228, 1331
Tsp45I	GTSAC	2 cut(s)	9, 1340
TspDTI	ATGAA	7 cut(s)	598, 810, 933, 1130, 1314, 1532, 1546
TspGWI	ACGGA	2 cut(s)	1368, 1479
TspRI	CASTG	3 cut(s)	18, 206, 301
VneI	GTGCAC	1 cut(s)	387
VpaK11BI	GGWCC	2 cut(s)	73, 97
XapI	RAATTY	5 cut(s)	566, 605, 684, 1019, 1359
XceI	RCATGY	3 cut(s)	1001, 1440, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	301
XmnI	GAANNNNTTC	2 cut(s)	1362, 1523
XspI	CTAG	3 cut(s)	615, 827, 1373
Zsp2I	ATGCAT	1 cut(s)	1442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.