Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0013167

Physical Location & Seq

Genomic Coordinates Reverse (-)

30074447 .. 30083500

Gene Structure 9054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1234100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3147 bp

ATGTCGCAGTCACTGGAGCTCTTGCTGATACAATTCCTGATGCCGGACAACGACGCCCGGCGGCAGGCGGAGGACCAGATAAAGCGCTTGGCCAAGGACCCTCAGGTGGTACCCGCGCTCGTACAGCACCTCCGCACCGCCAAGACGCCGAACGTGAGGCAATTAGCGGCAGTGCTTCTGAGGAAGAAGATCACCGGACACTGGGCCAAGCTCTCACCTCAAATTAAACACCTCGTTAAGCAGTCCCTCATTGAGAGCATCACAATGGAGCACAGTCCGCCGGTGAGGCGAGCCAGTGCCAATGTGGTTAGTGTTGTCGCAAAATATGCAGTCCCGGCTGGAGAATGGCCGGATTTGTTGCCCTTTCTGTTTCAATGTAGTCAGAGTGCACAGGAGGAACATAGAGAGGTGGCGTTGATATTGTTCAGCTCTTTGACTGAAACAATAGGGAATACGTTTCGGCCACATTTCGCAGATTTGCAAGCTCTTCTTCTGAAATGCCTGCAGGATGAGACAAGCAACCGCGTCAGAGTTGCTGCGCTCAAGGCCGTAGGGTCTTTTCTGGAATTCACTCATGATGGGGTTGAAGTGGTGAAGTTTCGGGAATTTATACCTAGCATCTTAAATGTATCAAGACAATGCCTTGCAGCTGGCGAGGAGGATGTTGCTATTATTGCTTTTGAAATTTTTGATGAGTTGATTGAATCTCCCGCACCTCTTCTTGGTGAATCTGTTAAATCCATTGTGCAATTCTCTCTTGAAGTTTGTTCAACTCAAAGTTTAGAATCTAATACACGTCATCAGGCAATTCAGATAGTTTCATGGCTAGCAAAGTATAAATCCAATTCCCTCAAAAAGCATAAGCTGGTCATCCCTATTCTGCAAGTTATGTGCCCATTGCTTGCAGAATCAAATGATGAAGATAAGGATGATGATCTTGCTCCAGATCGAGCTGCTGCAGAAGTTATTGATACTATGGCTTTGAACATACCAAAGCATGTTTTCCACCCTGTCTTTGAATTTTCTTCTTTAAGCAGTAAAAATGCAAATCCAAAATATCGGGAAGCGTCTGTTACCGCTTTAGGTGTCATTTCAGAGGGTTGTTTGGAGATGATAAAAGATAAGTTGGATCCAGTTCTTCATATTGTCTTAGGCGCTCTGAGAGATCCGGAGGAAATGGTTAGGGGGGCTGCATCATTTGCATTGGGTCAGTTTGCAGAGCATTTGCAGCCGGAAATTGTTTCCCACTATCAAAGTGTACTTCCCTGCATTTTGATTGCCCTTGAGGATGCATCTGACGAAGTGAAGGAGAAGTCATATTATGCTTTGGCAGCATTTTGTGACAACATGGGTGAGGAAATTCTTCCATTCCTTGATCCGTTGATGGGAAAACTACTGGGGGCCCTCCATAATAGCCCTCGTAATTTGCAGGAGACATGCATGTCTGCGATTGGTTCGGTTGCATCTGCGGCAGAACAGGCATTCATTCCGTATGCTGAAAGGGTTTTGGAGTTGATGAAAAGTTTCTTGGTGCTTAGTAATGATGAGGATCTTCGTTCACGAGCAAGAGCTACTGAATTAGTTGGAATTGTTGCAATGTGTGTGGGGAGAACTAGGATGGAACCAATTTTACCCCCTTATATAGAAGCTGCAATTTCTGGTTTTGGATTGGATTTCAGTGAACTTCGGGAGTATATTCATGGATTCTTTAGCAATGTGGTAGAAATTTTGGATGACAGCTTTATACAGTATCTTCCTCATGTTGTACCCCTGGCGTTTTCTTCCTGCAATCTTGATGATGGAGCTGCAGTGGACATCGATGAGTCTGATGATGAAAATATTAATGGATTTGGTGGAGTTTCATCTGATGATGAAGCTCATGATGAGCCAAGAGTACGAAATATCAGCGTAAGAACAGGAGTTTTAGATGAAAAGGCAGCTGCAACTCAAGCTCTTGGCTTATTTGCACTACATACGAAGGCCTCATATGCACCCTATTTGGAGGAATCATTTAAGATCCTAGTACGACACTCAGGGTATTTTCATGAAGATGTTCGGCTTCAGGCAATCATTTCTCTGAAACATATTTTAACAGCAGCACAGGCAGTTTACCAGAATCATAATGAAGGACAAGCAAGGGCTAAGGAAATTCTGGATACTGTGATGAACACTTATATCAAGACTATGACTGAAGATGATGACAAGGAAGTTGTTGCTCAAGCTTGTATGAGCCTTGCTGATATCATCAAAGATTATGGATATATGGCTGTTGAGCCTTATGTACCACGGGTTGTTGATGCTACTTTGGTACTGCTTCGGGAGGAATCAGCTTGTCAGCAGACAGAATCTGATGATGAAATTGATGATGATGATGTTGTACATGATGAAGAACTTATGGATGCAGTTTCTGACCTTCTTCCTGCTTTTGCAAAGTCAATGGGTCCTCATTTTGCACCTATTTTTGCTACGCTATTTGAACCTTTAATGAAATTTGCGAGAGCATCGCAGCCACTGCAAGATCGGACAATGGTTGTTGCCTGCCTTGCTGAAGTTGCTCAAGACATGGGTGCTCCAATTGCTGGATATGTCGATAGGGTGATGCCCTTGGTAATCAAAGAACTAGCATCCTCAGACGCAACCAATAGAAGGAATGCTGCATTTTGTGTTGGAGAGCTGTGCAAAAATGGGGGTGAGGGGACGTTGAAATACTATGGTGATATATTGCGTGGACTTTACCCACTATTTGGGGAATCTGAGCCAGATGATGCTGTCAGGGATAACGCAGCTGGTGCAGTGGCAAGAATGATAATGGTGCACCCTGAATCTATCCCATTGAATCAGGTACTTCCTGTTTTCTTGAAGGTTCTTCCACTAAAAGAAGATCGTGAAGAGTCCATGACGGTGTATAGTTGCGTCTCTACTCTTGTTTTATCATCTAATGCCCAGATCCTTTCTCTAGTCCCAGATCTGGTTAATGTATTTGCTCAAGTTGTGGCATCACCAGTTGAAACTCCTGAAGTAAAAGCACAAATCGGAAGAGCTTTTGCTCACCTGATTTCACTCTATGGCCAACAAATGCAGCCCCTTTTGAGTAACCTCTCCCCTGCTTATGCAAATGCCCTAGCTGCATTTGTCCCGAAAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000060 GO:0003674 GO:0005048 GO:0005215 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0006606 GO:0006607 GO:0006610 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008139 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0017038 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0033036 GO:0033218 GO:0033365 GO:0034399 GO:0034504 GO:0034613 GO:0042277 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0061608 GO:0070013 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HEAT IBN_N Vac14_Fab1_bd

Protein Analysis

1049

Amino Acids

115.38

Weight (kDa)

4.87

Isoelectric Point (pI)

47.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	23 - 87	2.1e-15	Importin-beta N-terminal domain
TPR_IPO5	PF25780	93 - 251	4.5e-33	IPO5-like, TPR repeats
HEAT_GCN1	PF23271	272 - 447	9.6e-06	Stalled ribosome sensor GCN1-like, HEAT repeats
TOR1L1_N	PF24714	317 - 534	1.4e-08	TORTIFOLIA1 N-terminal
HEAT_EZ	PF13513	351 - 405	5.1e-06	HEAT-like repeat
TPR_IMB1	PF25574	370 - 515	6.8e-10	Importin subunit beta-1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013167)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27640
fragaria_vesca	FvH4_5g29150
malus_domestica	MD08G1233900.v1.1 MD08G1234100.v1.1 MD15G1425400.v1.1
prunus_persica	Prupe.1G571100_v2.0.a1 Prupe.1G571100_v2.0.a1
pyrus_communis	pycom08g20240 pycom15g37710
rosa_chinensis	RchiOBHm_Chr7g0221821
rosa_laevigata	RLG00000002119
rosa_multiflora	Rmu_sc0005834.1_g000014
rosa_roxburghii	Rroxscaffold_3G00237670
rosa_rugosa	Rorug07G0203600
rosa_samantha	Rh7BG335300 Rh7CG362100 Rh7DG342400
rosa_wichuraiana	Rw7G029220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1441
Acc65I	GGTACC	1 cut(s)	109
AccB1I	GGYRCC	1 cut(s)	109
AccB7I	CCANNNNNTGG	2 cut(s)	2579, 2744
AccII	CGCG	2 cut(s)	116, 525
AccIII	TCCGGA	1 cut(s)	1168
AclWI	GGATC	7 cut(s)	1124, 1137, 1160, 1370, 1557, 2011, 2941
AcoI	YGGCCR	4 cut(s)	90, 347, 461, 3067
AcsI	RAATTY	8 cut(s)	566, 605, 684, 1019, 1359, 1725, 2148, 2489
AcuI	CTGAAG	4 cut(s)	2045, 2211, 2568, 3036
AcyI	GRCGYC	2 cut(s)	54, 146
AfeI	AGCGCT	1 cut(s)	86
AflIII	ACRYGT	1 cut(s)	794
AjiI	CACGTC	1 cut(s)	797
AjnI	CCWGG	1 cut(s)	1770
AjuI	GAANNNNNNNTTGG	2 cut(s)	1511, 1543
Alw21I	GWGCWC	5 cut(s)	21, 273, 391, 2572, 2817
Alw26I	GTCTC	3 cut(s)	506, 1427, 2920
Alw44I	GTGCAC	2 cut(s)	387, 2813
AlwI	GGATC	7 cut(s)	1124, 1137, 1160, 1370, 1557, 2011, 2941
AlwNI	CAGNNNCTG	4 cut(s)	13, 2348, 2408, 2512
Aor13HI	TCCGGA	1 cut(s)	1168
Aor51HI	AGCGCT	1 cut(s)	86
AoxI	GGCC	8 cut(s)	90, 204, 347, 461, 546, 1401, 1980, 3067
ApaI	GGGCCC	1 cut(s)	1405
ApaLI	GTGCAC	2 cut(s)	387, 2813
ApoI	RAATTY	8 cut(s)	566, 605, 684, 1019, 1359, 1725, 2148, 2489
AseI	ATTAAT	1 cut(s)	1842
Asp700I	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
Asp718I	GGTACC	1 cut(s)	109
AspLEI	GCGC	4 cut(s)	87, 118, 541, 1157
AspS9I	GGNCC	6 cut(s)	73, 97, 204, 1401, 1402, 2441
AsuC2I	CCSGG	2 cut(s)	58, 335
AsuNHI	GCTAGC	1 cut(s)	826
AvaII	GGWCC	3 cut(s)	73, 97, 2441
AxyI	CCTNAGG	1 cut(s)	102
BaeGI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BalI	TGGCCA	2 cut(s)	92, 3069
BamHI	GGATCC	1 cut(s)	1129
BanI	GGYRCC	1 cut(s)	109
BanII	GRGCYC	2 cut(s)	21, 1405
BauI	CACGAG	1 cut(s)	1560
Bbv12I	GWGCWC	5 cut(s)	21, 273, 391, 2572, 2817
BccI	CCATC	4 cut(s)	572, 1378, 1612, 1793
BceAI	ACGGC	1 cut(s)	533
BciT130I	CCWGG	1 cut(s)	1772
BciVI	GTATCC	1 cut(s)	2149
BcnI	CCSGG	2 cut(s)	58, 335
BcoDI	GTCTC	3 cut(s)	506, 1427, 2920
BfaI	CTAG	7 cut(s)	615, 827, 1614, 2021, 2621, 2957, 3122
BfmI	CTRYAG	3 cut(s)	503, 957, 1806
BfoI	RGCGCY	2 cut(s)	88, 1158
BfuI	GTATCC	1 cut(s)	2149
BglI	GCCNNNNNGGC	1 cut(s)	286
BglII	AGATCT	1 cut(s)	2965
Bme1390I	CCNGG	3 cut(s)	58, 335, 1772
Bme18I	GGWCC	3 cut(s)	73, 97, 2441
BmgBI	CACGTC	1 cut(s)	797
BmgT120I	GGNCC	6 cut(s)	73, 97, 204, 1401, 1402, 2441
BmiI	GGNNCC	7 cut(s)	99, 111, 1131, 1402, 1403, 1623, 2442
BmrFI	CCNGG	3 cut(s)	58, 335, 1772
BmrI	ACTGGG	2 cut(s)	211, 1406
BmtI	GCTAGC	1 cut(s)	830
BmuI	ACTGGG	2 cut(s)	211, 1406
BpmI	CTGGAG	3 cut(s)	35, 360, 927
Bpu10I	CCTNAGC	1 cut(s)	2142
BpuEI	CTTGAG	6 cut(s)	527, 1304, 1932, 2202, 2541, 2970
BpuMI	CCSGG	2 cut(s)	58, 335
Bsa29I	ATCGAT	1 cut(s)	1818
BsaBI	GATNNNNATC	2 cut(s)	933, 1548
BsaHI	GRCGYC	2 cut(s)	54, 146
BsaJI	CCNNGG	4 cut(s)	93, 1770, 2285, 2604
BsaWI	WCCGGW	2 cut(s)	194, 1168
BsaXI	ACNNNNNCTCC	4 cut(s)	114, 144, 1794, 1824
Bse118I	RCCGGY	1 cut(s)	280
Bse1I	ACTGG	6 cut(s)	18, 206, 294, 1133, 1401, 3002
Bse21I	CCTNAGG	1 cut(s)	102
Bse3DI	GCAATG	3 cut(s)	896, 1602, 1720
Bse8I	GATNNNNATC	2 cut(s)	933, 1548
BseAI	TCCGGA	1 cut(s)	1168
BseBI	CCWGG	1 cut(s)	1772
BseCI	ATCGAT	1 cut(s)	1818
BseDI	CCNNGG	4 cut(s)	93, 1770, 2285, 2604
BseGI	GGATG	9 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2404, 2624
BseJI	GATNNNNATC	2 cut(s)	933, 1548
BseMI	GCAATG	3 cut(s)	896, 1602, 1720
BseMII	CTCAG	6 cut(s)	116, 170, 1151, 2046, 2643, 2745
BseNI	ACTGG	6 cut(s)	18, 206, 294, 1133, 1401, 3002
BseRI	GAGGAG	1 cut(s)	671
BseSI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BsgI	GTGCAG	1 cut(s)	2811
Bsh1236I	CGCG	2 cut(s)	116, 525
BshFI	GGCC	8 cut(s)	92, 206, 349, 463, 548, 1403, 1982, 3069
BshNI	GGYRCC	1 cut(s)	109
BshVI	ATCGAT	1 cut(s)	1818
BsiHKAI	GWGCWC	5 cut(s)	21, 273, 391, 2572, 2817
BsiSI	CCGG	8 cut(s)	44, 58, 195, 281, 335, 350, 1169, 1232
BslFI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
BsmAI	GTCTC	3 cut(s)	506, 1427, 2920
BsmBI	CGTCTC	1 cut(s)	2920
BsmFI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
BsmI	GAATGC	2 cut(s)	1481, 2656
BsnI	GGCC	8 cut(s)	92, 206, 349, 463, 548, 1403, 1982, 3069
Bsp120I	GGGCCC	1 cut(s)	1401
Bsp1286I	GDGCHC	7 cut(s)	21, 273, 391, 896, 1405, 2572, 2817
Bsp13I	TCCGGA	1 cut(s)	1168
Bsp1407I	TGTACA	1 cut(s)	2377
BspANI	GGCC	8 cut(s)	92, 206, 349, 463, 548, 1403, 1982, 3069
BspCNI	CTCAG	6 cut(s)	115, 171, 1152, 2045, 2642, 2746
BspDI	ATCGAT	1 cut(s)	1818
BspEI	TCCGGA	1 cut(s)	1168
BspFNI	CGCG	2 cut(s)	116, 525
BspHI	TCATGA	3 cut(s)	574, 1879, 2044
BspLI	GGNNCC	7 cut(s)	99, 111, 1131, 1402, 1403, 1623, 2442
BspMAI	CTGCAG	3 cut(s)	507, 961, 1810
BspOI	GCTAGC	1 cut(s)	830
BspPI	GGATC	7 cut(s)	1124, 1137, 1160, 1370, 1557, 2011, 2941
BspQI	GCTCTTC	2 cut(s)	492, 3031
BspT107I	GGYRCC	1 cut(s)	109
BsrDI	GCAATG	3 cut(s)	896, 1602, 1720
BsrFI	RCCGGY	1 cut(s)	280
BsrGI	TGTACA	1 cut(s)	2377
BsrI	ACTGG	6 cut(s)	18, 206, 294, 1133, 1401, 3002
BssAI	RCCGGY	1 cut(s)	280
BssECI	CCNNGG	4 cut(s)	93, 1770, 2285, 2604
BssNI	GRCGYC	2 cut(s)	54, 146
BssSI	CACGAG	1 cut(s)	1560
BssT1I	CCWWGG	2 cut(s)	93, 2604
Bst2BI	CACGAG	1 cut(s)	1560
Bst2UI	CCWGG	1 cut(s)	1772
Bst4CI	ACNGT	4 cut(s)	275, 1749, 2161, 2902
Bst6I	CTCTTC	4 cut(s)	492, 723, 2883, 3031
BstACI	GRCGYC	2 cut(s)	54, 146
BstAPI	GCANNNNNTGC	4 cut(s)	326, 1199, 2512, 2789
BstAUI	TGTACA	1 cut(s)	2377
BstC8I	GCNNGC	8 cut(s)	66, 291, 483, 503, 652, 828, 903, 2539
BstDEI	CTNAG	9 cut(s)	102, 179, 1150, 1160, 1535, 2032, 2142, 2629, 2754
BstDSI	CCRYGG	1 cut(s)	2285
BstF5I	GGATG	9 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2404, 2624
BstFNI	CGCG	2 cut(s)	116, 525
BstH2I	RGCGCY	2 cut(s)	88, 1158
BstHHI	GCGC	4 cut(s)	87, 118, 541, 1157
BstMAI	GTCTC	3 cut(s)	506, 1427, 2920
BstNI	CCWGG	1 cut(s)	1772
BstNSI	RCATGY	3 cut(s)	1001, 1440, 1444
BstSCI	CCNGG	3 cut(s)	56, 333, 1770
BstSFI	CTRYAG	3 cut(s)	503, 957, 1806
BstSLI	GKGCMC	4 cut(s)	391, 896, 1405, 2817
BstUI	CGCG	2 cut(s)	116, 525
BstX2I	RGATCY	6 cut(s)	1129, 1165, 1549, 2016, 2946, 2965
BstYI	RGATCY	6 cut(s)	1129, 1165, 1549, 2016, 2946, 2965
Bsu15I	ATCGAT	1 cut(s)	1818
Bsu36I	CCTNAGG	1 cut(s)	102
BsuI	GTATCC	1 cut(s)	2149
BsuRI	GGCC	8 cut(s)	92, 206, 349, 463, 548, 1403, 1982, 3069
BsuTUI	ATCGAT	1 cut(s)	1818
BtgI	CCRYGG	1 cut(s)	2285
BtgZI	GCGATG	1 cut(s)	2487
BtrI	CACGTC	1 cut(s)	797
BtsCI	GGATG	9 cut(s)	514, 667, 870, 934, 1294, 1623, 1738, 2404, 2624
BtsI	GCAGTG	4 cut(s)	177, 1815, 2510, 2799
BtsIMutI	CAGTG	8 cut(s)	11, 177, 199, 301, 1684, 1815, 2510, 2799
Cac8I	GCNNGC	8 cut(s)	66, 291, 483, 503, 652, 828, 903, 2539
CaiI	CAGNNNCTG	4 cut(s)	13, 2348, 2408, 2512
CciI	TCATGA	3 cut(s)	574, 1879, 2044
CfoI	GCGC	4 cut(s)	87, 118, 541, 1157
Cfr10I	RCCGGY	1 cut(s)	280
Cfr13I	GGNCC	6 cut(s)	73, 97, 204, 1401, 1402, 2441
ClaI	ATCGAT	1 cut(s)	1818
CseI	GACGC	6 cut(s)	62, 154, 514, 1056, 2642, 2902
CspCI	CAANNNNNGTGG	2 cut(s)	1584, 1619
DdeI	CTNAG	9 cut(s)	102, 179, 1150, 1160, 1535, 2032, 2142, 2629, 2754
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	4 cut(s)	90, 347, 461, 3067
Eam1104I	CTCTTC	4 cut(s)	492, 723, 2883, 3031
EarI	CTCTTC	4 cut(s)	492, 723, 2883, 3031
EciI	GGCGGA	2 cut(s)	83, 267
Ecl136II	GAGCTC	1 cut(s)	19
Eco130I	CCWWGG	2 cut(s)	93, 2604
Eco147I	AGGCCT	1 cut(s)	1982
Eco24I	GRGCYC	2 cut(s)	21, 1405
Eco32I	GATATC	1 cut(s)	2242
Eco47I	GGWCC	3 cut(s)	73, 97, 2441
Eco47III	AGCGCT	1 cut(s)	86
Eco53kI	GAGCTC	1 cut(s)	19
Eco57I	CTGAAG	4 cut(s)	2045, 2211, 2568, 3036
Eco81I	CCTNAGG	1 cut(s)	102
EcoICRI	GAGCTC	1 cut(s)	19
EcoO109I	RGGNCCY	4 cut(s)	97, 1401, 1402, 2441
EcoRI	GAATTC	1 cut(s)	566
EcoRII	CCWGG	1 cut(s)	1770
EcoRV	GATATC	1 cut(s)	2242
EcoT14I	CCWWGG	2 cut(s)	93, 2604
EcoT22I	ATGCAT	2 cut(s)	1294, 1442
EcoT38I	GRGCYC	2 cut(s)	21, 1405
ErhI	CCWWGG	2 cut(s)	93, 2604
Esp3I	CGTCTC	1 cut(s)	2920
FalI	AAGNNNNNCTT	2 cut(s)	474, 506
FaqI	GGGAC	5 cut(s)	229, 317, 2710, 2945, 3119
FauI	CCCGC	2 cut(s)	121, 718
FauNDI	CATATG	1 cut(s)	1987
FokI	GGATG	9 cut(s)	521, 674, 857, 941, 1301, 1630, 1745, 2411, 2611
FriOI	GRGCYC	2 cut(s)	21, 1405
FspBI	CTAG	7 cut(s)	615, 827, 1614, 2021, 2621, 2957, 3122
GlaI	GCGC	4 cut(s)	86, 117, 540, 1156
GsuI	CTGGAG	3 cut(s)	35, 360, 927
HaeII	RGCGCY	2 cut(s)	88, 1158
HaeIII	GGCC	8 cut(s)	92, 206, 349, 463, 548, 1403, 1982, 3069
HapII	CCGG	8 cut(s)	44, 58, 195, 281, 335, 350, 1169, 1232
HgaI	GACGC	6 cut(s)	62, 154, 514, 1056, 2642, 2902
HhaI	GCGC	4 cut(s)	87, 118, 541, 1157
Hin1I	GRCGYC	2 cut(s)	54, 146
Hin6I	GCGC	4 cut(s)	85, 116, 539, 1155
HinP1I	GCGC	4 cut(s)	85, 116, 539, 1155
HindIII	AAGCTT	1 cut(s)	2220
HpaII	CCGG	8 cut(s)	44, 58, 195, 281, 335, 350, 1169, 1232
Hpy166II	GTNNAC	8 cut(s)	389, 1259, 1559, 1682, 1813, 2110, 2729, 2815
Hpy8I	GTNNAC	8 cut(s)	389, 1259, 1559, 1682, 1813, 2110, 2729, 2815
Hpy99I	CGWCG	1 cut(s)	56
HpyAV	CCTTC	6 cut(s)	1300, 1972, 2120, 2423, 2640, 2854
HpyCH4III	ACNGT	4 cut(s)	275, 1749, 2161, 2902
HpyCH4IV	ACGT	4 cut(s)	153, 455, 796, 2699
HpyF3I	CTNAG	9 cut(s)	102, 179, 1150, 1160, 1535, 2032, 2142, 2629, 2754
HpySE526I	ACGT	4 cut(s)	153, 455, 796, 2699
Hsp92I	GRCGYC	2 cut(s)	54, 146
HspAI	GCGC	4 cut(s)	85, 116, 539, 1155
Kpn2I	TCCGGA	1 cut(s)	1168
KpnI	GGTACC	1 cut(s)	113
LguI	GCTCTTC	2 cut(s)	492, 3031
LmnI	GCTCC	5 cut(s)	16, 268, 946, 1802, 2575
MaeI	CTAG	7 cut(s)	615, 827, 1614, 2021, 2621, 2957, 3122
MaeII	ACGT	4 cut(s)	153, 455, 796, 2699
MaeIII	GTNAC	4 cut(s)	9, 1072, 1340, 3092
MfeI	CAATTG	1 cut(s)	2574
MflI	RGATCY	6 cut(s)	1129, 1165, 1549, 2016, 2946, 2965
MhlI	GDGCHC	7 cut(s)	21, 273, 391, 896, 1405, 2572, 2817
MlsI	TGGCCA	2 cut(s)	92, 3069
MluNI	TGGCCA	2 cut(s)	92, 3069
MlyI	GAGTC	2 cut(s)	1832, 2900
MmeI	TCCRAC	3 cut(s)	1107, 1564, 2647
Mox20I	TGGCCA	2 cut(s)	92, 3069
Mph1103I	ATGCAT	2 cut(s)	1294, 1442
MroI	TCCGGA	1 cut(s)	1168
MroXI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
MscI	TGGCCA	2 cut(s)	92, 3069
MslI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
Msp20I	TGGCCA	2 cut(s)	92, 3069
MspA1I	CMGCKG	3 cut(s)	650, 1940, 2786
MspI	CCGG	8 cut(s)	44, 58, 195, 281, 335, 350, 1169, 1232
MspR9I	CCNGG	3 cut(s)	58, 335, 1772
MunI	CAATTG	1 cut(s)	2574
Mva1269I	GAATGC	2 cut(s)	1481, 2656
MvaI	CCWGG	1 cut(s)	1772
MvnI	CGCG	2 cut(s)	116, 525
NciI	CCSGG	2 cut(s)	58, 335
NdeI	CATATG	1 cut(s)	1987
NheI	GCTAGC	1 cut(s)	826
NlaIV	GGNNCC	7 cut(s)	99, 111, 1131, 1402, 1403, 1623, 2442
NmuCI	GTSAC	2 cut(s)	9, 1340
NsiI	ATGCAT	2 cut(s)	1294, 1442
NspI	RCATGY	3 cut(s)	1001, 1440, 1444
PagI	TCATGA	3 cut(s)	574, 1879, 2044
PceI	AGGCCT	1 cut(s)	1982
PciSI	GCTCTTC	2 cut(s)	492, 3031
PctI	GAATGC	2 cut(s)	1481, 2656
PdmI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
PflMI	CCANNNNNTGG	2 cut(s)	2579, 2744
PleI	GAGTC	2 cut(s)	1831, 2899
PpsI	GAGTC	2 cut(s)	1831, 2899
PpuMI	RGGWCCY	2 cut(s)	97, 2441
PshBI	ATTAAT	1 cut(s)	1842
Psp124BI	GAGCTC	1 cut(s)	21
Psp5II	RGGWCCY	2 cut(s)	97, 2441
Psp6I	CCWGG	1 cut(s)	1770
PspGI	CCWGG	1 cut(s)	1770
PspN4I	GGNNCC	7 cut(s)	99, 111, 1131, 1402, 1403, 1623, 2442
PspOMI	GGGCCC	1 cut(s)	1401
PspPI	GGNCC	6 cut(s)	73, 97, 204, 1401, 1402, 2441
PspPPI	RGGWCCY	2 cut(s)	97, 2441
PsrI	GAACNNNNNNTAC	2 cut(s)	1614, 1646
PstI	CTGCAG	3 cut(s)	507, 961, 1810
PstNI	CAGNNNCTG	4 cut(s)	13, 2348, 2408, 2512
PsuI	RGATCY	6 cut(s)	1129, 1165, 1549, 2016, 2946, 2965
PvuII	CAGCTG	3 cut(s)	650, 1940, 2786
RseI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
SacI	GAGCTC	1 cut(s)	21
SapI	GCTCTTC	2 cut(s)	492, 3031
Sau96I	GGNCC	6 cut(s)	73, 97, 204, 1401, 1402, 2441
SbfI	CCTGCAGG	1 cut(s)	507
SchI	GAGTC	2 cut(s)	1832, 2900
ScrFI	CCNGG	3 cut(s)	58, 335, 1772
SdaI	CCTGCAGG	1 cut(s)	507
SduI	GDGCHC	7 cut(s)	21, 273, 391, 896, 1405, 2572, 2817
SfcI	CTRYAG	3 cut(s)	503, 957, 1806
SgrAI	CRCCGGYG	1 cut(s)	280
SinI	GGWCC	3 cut(s)	73, 97, 2441
SmiMI	CAYNNNNRTG	3 cut(s)	263, 2049, 2900
SmlI	CTYRAG	6 cut(s)	542, 1283, 1947, 2217, 2556, 2985
SmoI	CTYRAG	6 cut(s)	542, 1283, 1947, 2217, 2556, 2985
Sse8387I	CCTGCAGG	1 cut(s)	507
SseBI	AGGCCT	1 cut(s)	1982
SspI	AATATT	1 cut(s)	1840
SspMI	CTAG	7 cut(s)	615, 827, 1614, 2021, 2621, 2957, 3122
SstI	GAGCTC	1 cut(s)	21
StuI	AGGCCT	1 cut(s)	1982
StyD4I	CCNGG	3 cut(s)	56, 333, 1770
StyI	CCWWGG	2 cut(s)	93, 2604
TaaI	ACNGT	4 cut(s)	275, 1749, 2161, 2902
TaiI	ACGT	4 cut(s)	156, 458, 799, 2702
TaqI	TCGA	3 cut(s)	949, 1818, 2589
TatI	WGTACW	2 cut(s)	1258, 2377
TauI	GCSGC	3 cut(s)	64, 170, 1472
TscAI	CASTG	8 cut(s)	18, 177, 206, 301, 1684, 1815, 2517, 2799
TseFI	GTSAC	2 cut(s)	9, 1340
Tsp45I	GTSAC	2 cut(s)	9, 1340
TspGWI	ACGGA	2 cut(s)	1368, 1479
TspRI	CASTG	8 cut(s)	18, 177, 206, 301, 1684, 1815, 2517, 2799
Van91I	CCANNNNNTGG	2 cut(s)	2579, 2744
VneI	GTGCAC	2 cut(s)	387, 2813
VpaK11BI	GGWCC	3 cut(s)	73, 97, 2441
VspI	ATTAAT	1 cut(s)	1842
XapI	RAATTY	8 cut(s)	566, 605, 684, 1019, 1359, 1725, 2148, 2489
XceI	RCATGY	3 cut(s)	1001, 1440, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	301
XmnI	GAANNNNTTC	3 cut(s)	1362, 1523, 2052
XspI	CTAG	7 cut(s)	615, 827, 1614, 2021, 2621, 2957, 3122
Zsp2I	ATGCAT	2 cut(s)	1294, 1442

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.