AT4G28100

gpi-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011246
Physical Location & Seq
Reverse (-)
13965099 .. 13967076
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28100.1

Sequence Viewer

Length: 915 bp
ATGAAGAAAAGTCTCACTCTACTCATCCTCCTCCTCTGTTCTCTCCTCTTCTCCACCGTCTTGAGCAACCTACTCGTCGAACCGGTCCAACCCAACACCGTACCAGCCTTCCCCGTCGAAACCCAAGCTCAAAGCTGCCGACTTGACCTCTCCAACGAGCTATTCGGTGGCGTCAACGAAGCTTGCGGAAGAAACCTTGACCGGAGTCGATGTTGCCCTGTTTTAGCCGCTTGGCTCTTCGCAGCTCACGCTCGCTCTGCTCTCCAGTTACCAGCTCCTGCTCCAACGCCGGAATCTTCTGATCCCGACGAACCCATGAAGCCTGACGACTCTCAGAAGTGCGTCAACACATTGCAAAGTGCGCTTTTGACTAAACAAATCAAGATCCCTCAGCCTAATTCTAGCTGTGACGCAATCTTGTGCTTCTGTGGTATTCGTCTCCACCAGATTAGCTCACTCTCTTGTCCCGCCGCTTTCAATGTCTCCTCCGGCTTCAAAAACGCAACTCCGACCGCCGCCGTGAAGAATCTTGAAAAAGAATGCCGGAACTCTTCTTACTCCGGTTGTACCAGATGCCTCGGTGCTCTTCAAAAGCTAAAGGTAAGAGGAGGAAACAAGAAGACAACAACAGAGAGAGGGACTAAGATGATGAGCAAAGATTGCCAGCTCATGGGACTCACATGGCTACTAGCTCGTAACAAAACGGCATACATTCCCACTGTTTCGGCTGTGTTAAGAGCTATTATGTATAGTCCACACCCACCTCACCTCAACAAGTGTAGTCCTGACCAAGAGAACATGCCTTTAGCAGTTGACTCTCTACAGTTTCAAAAGAGCTTCTCTTCATCATCTCATCTTTTTGGGGTTTTACCGTTTTTACCCTTAGTCCTTTGTATCTTTCTCTTTCTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

304

Amino Acids

33.1

Weight (kDa)

8.93

Isoelectric Point (pI)

60.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28100
fragaria_vesca FvH4_6g44390
malus_domestica MD09G1097800.v1.1 MD17G1086100.v1.1
prunus_persica Prupe.3G228400_v2.0.a1
pyrus_communis pycom09g02190 pycom17g08360
rosa_chinensis RchiOBHm_Chr2g0161331 RchiOBHm_Chr2g0161381
rosa_laevigata RLG00000021280
rosa_multiflora Rmu_co8294437.1_g000001 Rmu_ssc0000196.1_g000002
rosa_roxburghii Rroxscaffold_2G00088890
rosa_rugosa Rorug02G0488500
rosa_samantha Rh2AG555000 Rh2BG568200 Rh2CG538700 Rh2CG538800 Rh2DG577900
rosa_wichuraiana Rw2G045920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 670
AciI CCGC 6 cut(s) 186, 228, 468, 471, 513, 516
AclWI GGATC 2 cut(s) 296, 379
AcyI GRCGYC 1 cut(s) 171
AfaI GTAC 2 cut(s) 102, 568
AfiI CCNNNNNNNGG 1 cut(s) 670
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 5 cut(s) 478, 496, 533, 590, 830
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 3 cut(s) 17, 443, 487
AlwI GGATC 2 cut(s) 296, 379
AlwNI CAGNNNCTG 1 cut(s) 278
ApeKI GCWGC 2 cut(s) 135, 242
AsiGI ACCGGT 1 cut(s) 82
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 85
AsuHPI GGTGA 1 cut(s) 758
AvaII GGWCC 1 cut(s) 85
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 586
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 122, 254
BceAI ACGGC 2 cut(s) 503, 720
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BcoDI GTCTC 3 cut(s) 17, 443, 487
BfaI CTAG 2 cut(s) 402, 689
BfmI CTRYAG 1 cut(s) 821
BisI GCNGC 5 cut(s) 136, 228, 243, 471, 516
BlsI GCNGC 5 cut(s) 137, 229, 244, 472, 517
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmsI GCATC 1 cut(s) 563
BoxI GACNNNNGTC 1 cut(s) 204
BpiI GAAGAC 1 cut(s) 626
BpmI CTGGAG 1 cut(s) 248
Bpu10I CCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 1 cut(s) 577
BsaWI WCCGGW 3 cut(s) 82, 201, 560
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 196, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 670
Bse118I RCCGGY 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 350
BseDI CCNNGG 1 cut(s) 577
BseGI GGATG 1 cut(s) 24
BseLI CCNNNNNNNGG 1 cut(s) 670
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 2 cut(s) 347, 404
BseNI ACTGG 1 cut(s) 265
BseRI GAGGAG 5 cut(s) 20, 23, 35, 475, 621
BseXI GCAGC 2 cut(s) 122, 254
Bsh1285I CGRYCG 1 cut(s) 513
BshTI ACCGGT 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 6 cut(s) 83, 202, 290, 489, 544, 561
BslFI GGGAC 3 cut(s) 450, 652, 687
BslI CCNNNNNNNGG 1 cut(s) 670
BsmAI GTCTC 3 cut(s) 17, 443, 487
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 3 cut(s) 450, 652, 687
BsmI GAATGC 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 2 cut(s) 301, 384
BspACI CCGC 6 cut(s) 186, 228, 468, 471, 513, 516
BspCNI CTCAG 2 cut(s) 346, 403
BspPI GGATC 2 cut(s) 296, 379
BspQI GCTCTTC 2 cut(s) 242, 591
BsrDI GCAATG 1 cut(s) 350
BsrFI RCCGGY 1 cut(s) 82
BsrI ACTGG 1 cut(s) 265
BssAI RCCGGY 1 cut(s) 82
BssECI CCNNGG 1 cut(s) 577
BssMI GATC 2 cut(s) 301, 384
BssNI GRCGYC 1 cut(s) 171
Bst4CI ACNGT 6 cut(s) 58, 100, 721, 825, 873, 912
Bst6I CTCTTC 5 cut(s) 53, 242, 556, 591, 847
BstACI GRCGYC 1 cut(s) 171
BstAPI GCANNNNNTGC 1 cut(s) 660
BstC8I GCNNGC 3 cut(s) 184, 253, 665
BstDEI CTNAG 4 cut(s) 333, 390, 642, 883
BstF5I GGATG 1 cut(s) 24
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 2 cut(s) 304, 387
BstMAI GTCTC 3 cut(s) 17, 443, 487
BstMBI GATC 2 cut(s) 301, 384
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 4 cut(s) 248, 257, 361, 660
BstNSI RCATGY 1 cut(s) 802
BstPAI GACNNNNGTC 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 821
BstV1I GCAGC 2 cut(s) 122, 254
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BtsCI GGATG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 717
Cac8I GCNNGC 3 cut(s) 184, 253, 665
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 364
Cfr10I RCCGGY 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 85
CseI GACGC 3 cut(s) 160, 331, 419
Csp6I GTAC 2 cut(s) 101, 567
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 4 cut(s) 316, 670, 681, 799
CviQI GTAC 2 cut(s) 101, 567
DdeI CTNAG 4 cut(s) 333, 390, 642, 883
DpnI GATC 2 cut(s) 303, 386
DpnII GATC 2 cut(s) 301, 384
Eam1104I CTCTTC 5 cut(s) 53, 242, 556, 591, 847
EarI CTCTTC 5 cut(s) 53, 242, 556, 591, 847
Eco47I GGWCC 1 cut(s) 85
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 4 cut(s) 319, 673, 684, 802
FaiI YATR 7 cut(s) 317, 671, 682, 709, 746, 750, 800
FaqI GGGAC 3 cut(s) 450, 652, 687
FatI CATG 4 cut(s) 315, 669, 680, 798
FauI CCCGC 1 cut(s) 475
Fnu4HI GCNGC 5 cut(s) 136, 228, 243, 471, 516
FokI GGATG 1 cut(s) 11
Fsp4HI GCNGC 5 cut(s) 136, 228, 243, 471, 516
FspBI CTAG 2 cut(s) 402, 689
GlaI GCGC 1 cut(s) 363
GluI GCNGC 5 cut(s) 136, 228, 243, 471, 516
GsuI CTGGAG 1 cut(s) 248
HapII CCGG 6 cut(s) 83, 202, 290, 489, 544, 561
HgaI GACGC 3 cut(s) 160, 331, 419
HhaI GCGC 1 cut(s) 364
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 4 cut(s) 319, 673, 684, 802
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 3 cut(s) 175, 346, 814
HindII GTYRAC 3 cut(s) 175, 346, 814
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 6 cut(s) 205, 293, 329, 526, 675, 815
HpaII CCGG 6 cut(s) 83, 202, 290, 489, 544, 561
HphI GGTGA 1 cut(s) 758
Hpy166II GTNNAC 4 cut(s) 175, 346, 755, 814
Hpy188I TCNGA 3 cut(s) 301, 336, 510
Hpy188III TCNNGA 5 cut(s) 61, 305, 382, 530, 785
Hpy8I GTNNAC 4 cut(s) 175, 346, 755, 814
Hpy99I CGWCG 3 cut(s) 80, 119, 311
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 6 cut(s) 58, 100, 721, 825, 873, 912
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 4 cut(s) 248, 257, 361, 660
HpyF3I CTNAG 4 cut(s) 333, 390, 642, 883
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 4 cut(s) 319, 673, 684, 802
HspAI GCGC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 301, 384
LguI GCTCTTC 2 cut(s) 242, 591
LmnI GCTCC 2 cut(s) 280, 286
Lsp1109I GCAGC 2 cut(s) 122, 254
LweI GCATC 1 cut(s) 563
MaeI CTAG 2 cut(s) 402, 689
MaeIII GTNAC 3 cut(s) 267, 407, 695
MalI GATC 2 cut(s) 303, 386
MboI GATC 2 cut(s) 301, 384
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 1 cut(s) 397
MlyI GAGTC 4 cut(s) 214, 323, 669, 809
MmeI TCCRAC 4 cut(s) 112, 177, 308, 533
MseI TTAA 1 cut(s) 734
MspI CCGG 6 cut(s) 83, 202, 290, 489, 544, 561
Mva1269I GAATGC 1 cut(s) 545
MwoI GCNNNNNNNGC 4 cut(s) 248, 257, 361, 660
NdeII GATC 2 cut(s) 301, 384
NlaIII CATG 4 cut(s) 319, 673, 684, 802
NmuCI GTSAC 1 cut(s) 407
NspI RCATGY 1 cut(s) 802
PciSI GCTCTTC 2 cut(s) 242, 591
PctI GAATGC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 293, 526
PflMI CCANNNNNTGG 1 cut(s) 670
PinAI ACCGGT 1 cut(s) 82
PkrI GCNGC 5 cut(s) 137, 229, 244, 472, 517
PleI GAGTC 4 cut(s) 213, 323, 669, 809
PpsI GAGTC 4 cut(s) 213, 323, 669, 809
PshAI GACNNNNGTC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 85
PsrI GAACNNNNNNTAC 2 cut(s) 539, 571
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 102, 568
RsaNI GTAC 2 cut(s) 101, 567
SapI GCTCTTC 2 cut(s) 242, 591
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 5 cut(s) 136, 228, 243, 471, 516
Sau3AI GATC 2 cut(s) 301, 384
Sau96I GGNCC 1 cut(s) 85
SchI GAGTC 4 cut(s) 214, 323, 669, 809
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 1 cut(s) 563
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 1 cut(s) 397
SsiI CCGC 6 cut(s) 186, 228, 468, 471, 513, 516
SspMI CTAG 2 cut(s) 402, 689
TaaI ACNGT 6 cut(s) 58, 100, 721, 825, 873, 912
TaqI TCGA 3 cut(s) 78, 117, 208
TasI AATT 1 cut(s) 397
TauI GCSGC 3 cut(s) 230, 473, 518
TfiI GAWTC 2 cut(s) 293, 526
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 2 cut(s) 135, 242
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 17, 332, 834
TspRI CASTG 1 cut(s) 724
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 85
XceI RCATGY 1 cut(s) 802
XspI CTAG 2 cut(s) 402, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.