MD17G1086100.v1.1

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011246
Physical Location & Seq
Forward (+)
7068321 .. 7070519
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1086100.v1.1.491

Sequence Viewer

Length: 981 bp
ATGTCATCATTTCTTCCCACCTCCGCTCTCACATTCTTCCTCCTCCTCTTATTAATATCCAGCTTCCACATTTCCCTCGCCGGTCTACTCGCCGAACCGGTCACCGGTCACCCCCAGCCGCTCAAACCGGGGGAATACTCCAGCCCCAACACCATCCCGGCCCTCCCGTCCCAAACCCAGACCCAAACCTGCCACCTTGACCTCTCCGCGGAGCTCTTCGGCGGTGTCAGCCAAGCATGCGGCCGAGACCTCGACCGGAGCCGGTGCTGCCCTGTTTTAGCCGCCTGGCTCTTCGCCGCCCACGCCAGATCAGCCCTCGAGCTCCCCTCGTCGGATACGGCTCCCTCGGCGGTGCCCGACCAGCCGATGATGCCGGACGACTCGCAGAAGTGCGTCAACTCGCTGCAGTCCTCGCTCGTCAGCCGGAACATTCGGATTCCTCAGCCCAACGCCAGCTGCGACGCCGTTCTGTGCTTCTGCGGAATCCGGCTCCACCAGATCGGCTCCCTGAGCTGCCCCGCTGCCTTCAACGTGTCTCGAGGATTTCACAACGCCACCCCCACCGCCGCCGTCAGGAATCTCGAGAAGAACTGCCGCAACTCTTCGTACTCTGGATGCACCAAGTGCCTTGGAGCTCTCCAGAAGCTCAAGGGAGGGAGCAAGAACGGAACGGCGGGGGAGAGGAGCACGAGCGAGAGAGCGAACAAGATGTTCAACCTTGACTGCCAGCTAATGGGGCTGACGTGGCTCCTCGCCCGGAACAAAACGGCGTACATTCCGACCGTTTCGGCCGTGTTGCGCGCCATAATGTACAGCGCGCACCCGCATCACGAGAACACGTGTAGCCCGGACCAGGAGAACATGCCGCTGGCCGTGGATTCGCTGCAGATCGAGAACGGGCAAGGGTTCGCCGCATCGCCGCCGTCCATCTTGATGTTACCGATTCTCCCTCTGATCATTTGGGCGTCTCTGTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

34.92

Weight (kDa)

7.93

Isoelectric Point (pI)

59.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011246)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28100
fragaria_vesca FvH4_6g44390
malus_domestica MD09G1097800.v1.1 MD17G1086100.v1.1
prunus_persica Prupe.3G228400_v2.0.a1
pyrus_communis pycom09g02190 pycom17g08360
rosa_chinensis RchiOBHm_Chr2g0161331 RchiOBHm_Chr2g0161381
rosa_laevigata RLG00000021280
rosa_multiflora Rmu_co8294437.1_g000001 Rmu_ssc0000196.1_g000002
rosa_roxburghii Rroxscaffold_2G00088890
rosa_rugosa Rorug02G0488500
rosa_samantha Rh2AG555000 Rh2BG568200 Rh2CG538700 Rh2CG538800 Rh2DG577900
rosa_wichuraiana Rw2G045920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 733
AccBSI CCGCTC 2 cut(s) 26, 121
AccI GTMKAC 1 cut(s) 85
AccII CGCG 3 cut(s) 209, 801, 818
AcoI YGGCCR 3 cut(s) 241, 789, 870
AcvI CACGTG 1 cut(s) 840
AcyI GRCGYC 2 cut(s) 462, 965
AdeI CACNNNGTG 1 cut(s) 624
AfaI GTAC 3 cut(s) 608, 773, 812
AfiI CCNNNNNNNGG 6 cut(s) 104, 208, 331, 573, 733, 853
AflIII ACRYGT 3 cut(s) 531, 837, 839
AgeI ACCGGT 2 cut(s) 97, 104
AgsI TTSAA 2 cut(s) 529, 715
AjiI CACGTC 1 cut(s) 744
AjnI CCWGG 2 cut(s) 284, 852
AluBI AGCT 8 cut(s) 63, 214, 322, 456, 513, 635, 646, 730
AluI AGCT 8 cut(s) 63, 214, 322, 456, 513, 635, 646, 730
Alw21I GWGCWC 4 cut(s) 216, 324, 637, 689
Alw26I GTCTC 3 cut(s) 240, 540, 972
Ama87I CYCGRG 3 cut(s) 317, 537, 581
AoxI GGCC 4 cut(s) 159, 241, 789, 870
ApeKI GCWGC 6 cut(s) 267, 403, 456, 513, 521, 883
AseI ATTAAT 1 cut(s) 53
AsiGI ACCGGT 2 cut(s) 97, 104
AspLEI GCGC 4 cut(s) 801, 803, 818, 820
AspS9I GGNCC 2 cut(s) 160, 850
AsuC2I CCSGG 4 cut(s) 129, 158, 757, 848
AsuHPI GGTGA 2 cut(s) 94, 101
AvaI CYCGRG 3 cut(s) 317, 537, 581
AvaII GGWCC 1 cut(s) 850
BaeGI GKGCMC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 352
BanII GRGCYC 3 cut(s) 216, 324, 637
BauI CACGAG 2 cut(s) 688, 830
BbrPI CACGTG 1 cut(s) 840
Bbv12I GWGCWC 4 cut(s) 216, 324, 637, 689
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 6 cut(s) 254, 390, 443, 500, 508, 870
BccI CCATC 2 cut(s) 161, 935
BceAI ACGGC 8 cut(s) 354, 449, 554, 687, 776, 783, 857, 907
BciT130I CCWGG 2 cut(s) 286, 854
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 1 cut(s) 954
BcnI CCSGG 4 cut(s) 129, 158, 757, 848
BcoDI GTCTC 3 cut(s) 240, 540, 972
BfmI CTRYAG 2 cut(s) 404, 884
BfuAI ACCTGC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 328
Bme1390I CCNGG 6 cut(s) 129, 158, 286, 757, 848, 854
Bme18I GGWCC 1 cut(s) 850
BmeT110I CYCGRG 3 cut(s) 317, 537, 581
BmgBI CACGTC 1 cut(s) 744
BmgT120I GGNCC 2 cut(s) 160, 850
BmiI GGNNCC 6 cut(s) 260, 342, 354, 491, 505, 749
BmrFI CCNGG 6 cut(s) 129, 158, 286, 757, 848, 854
BmsI GCATC 4 cut(s) 360, 605, 835, 923
BplI GAGNNNNNCTC 2 cut(s) 311, 343
BpmI CTGGAG 2 cut(s) 124, 623
Bpu10I CCTNAGC 2 cut(s) 441, 509
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 4 cut(s) 129, 158, 757, 848
BsaAI YACGTR 1 cut(s) 840
BsaHI GRCGYC 2 cut(s) 462, 965
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 5 cut(s) 128, 207, 345, 628, 873
BsaWI WCCGGW 3 cut(s) 97, 104, 255
BsaXI ACNNNNNCTCC 2 cut(s) 930, 960
Bsc4I CCNNNNNNNGG 6 cut(s) 104, 208, 331, 573, 733, 853
Bse118I RCCGGY 4 cut(s) 80, 97, 104, 261
BseBI CCWGG 2 cut(s) 286, 854
BseDI CCNNGG 5 cut(s) 128, 207, 345, 628, 873
BseGI GGATG 2 cut(s) 153, 620
BseLI CCNNNNNNNGG 6 cut(s) 104, 208, 331, 573, 733, 853
BseMII CTCAG 2 cut(s) 455, 500
BsePI GCGCGC 2 cut(s) 799, 816
BseRI GAGGAG 4 cut(s) 32, 35, 697, 740
BseSI GKGCMC 1 cut(s) 357
BseX3I CGGCCG 2 cut(s) 241, 789
BseXI GCAGC 6 cut(s) 254, 390, 443, 500, 508, 870
BseYI CCCAGC 1 cut(s) 114
Bsh1236I CGCG 3 cut(s) 209, 801, 818
Bsh1285I CGRYCG 4 cut(s) 244, 256, 783, 792
BshFI GGCC 4 cut(s) 161, 243, 791, 872
BshNI GGYRCC 1 cut(s) 352
BshTI ACCGGT 2 cut(s) 97, 104
BsiEI CGRYCG 4 cut(s) 244, 256, 783, 792
BsiHKAI GWGCWC 4 cut(s) 216, 324, 637, 689
BsiHKCI CYCGRG 3 cut(s) 317, 537, 581
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 6 cut(s) 104, 208, 331, 573, 733, 853
BsmAI GTCTC 3 cut(s) 240, 540, 972
BsmBI CGTCTC 1 cut(s) 972
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 4 cut(s) 161, 243, 791, 872
Bso31I GGTCTC 1 cut(s) 240
BsoBI CYCGRG 3 cut(s) 317, 537, 581
Bsp1286I GDGCHC 5 cut(s) 216, 324, 357, 637, 689
Bsp1407I TGTACA 1 cut(s) 810
Bsp143I GATC 4 cut(s) 308, 498, 888, 954
BspANI GGCC 4 cut(s) 161, 243, 791, 872
BspCNI CTCAG 2 cut(s) 454, 501
BspFNI CGCG 3 cut(s) 209, 801, 818
BspLI GGNNCC 6 cut(s) 260, 342, 354, 491, 505, 749
BspMAI CTGCAG 2 cut(s) 408, 888
BspMI ACCTGC 1 cut(s) 197
BspQI GCTCTTC 2 cut(s) 221, 296
BspT107I GGYRCC 1 cut(s) 352
BspTNI GGTCTC 1 cut(s) 240
BsrBI CCGCTC 2 cut(s) 26, 121
BsrFI RCCGGY 4 cut(s) 80, 97, 104, 261
BsrGI TGTACA 1 cut(s) 810
BssAI RCCGGY 4 cut(s) 80, 97, 104, 261
BssECI CCNNGG 5 cut(s) 128, 207, 345, 628, 873
BssHII GCGCGC 2 cut(s) 799, 816
BssMI GATC 4 cut(s) 308, 498, 888, 954
BssNI GRCGYC 2 cut(s) 462, 965
BssSI CACGAG 2 cut(s) 688, 830
BssT1I CCWWGG 1 cut(s) 628
Bst2BI CACGAG 2 cut(s) 688, 830
Bst2UI CCWGG 2 cut(s) 286, 854
Bst4CI ACNGT 1 cut(s) 784
Bst6I CTCTTC 3 cut(s) 221, 296, 607
BstACI GRCGYC 2 cut(s) 462, 965
BstAPI GCANNNNNTGC 1 cut(s) 624
BstAUI TGTACA 1 cut(s) 810
BstBAI YACGTR 1 cut(s) 840
BstC8I GCNNGC 6 cut(s) 238, 454, 728, 801, 818, 870
BstDEI CTNAG 2 cut(s) 441, 509
BstDSI CCRYGG 2 cut(s) 207, 873
BstEII GGTNACC 2 cut(s) 100, 107
BstF5I GGATG 2 cut(s) 153, 620
BstFNI CGCG 3 cut(s) 209, 801, 818
BstHHI GCGC 4 cut(s) 801, 803, 818, 820
BstKTI GATC 4 cut(s) 311, 501, 891, 957
BstMAI GTCTC 3 cut(s) 240, 540, 972
BstMBI GATC 4 cut(s) 308, 498, 888, 954
BstMCI CGRYCG 4 cut(s) 244, 256, 783, 792
BstNI CCWGG 2 cut(s) 286, 854
BstNSI RCATGY 2 cut(s) 240, 865
BstPI GGTNACC 2 cut(s) 100, 107
BstSCI CCNGG 6 cut(s) 127, 156, 284, 755, 846, 852
BstSFI CTRYAG 2 cut(s) 404, 884
BstSLI GKGCMC 1 cut(s) 357
BstUI CGCG 3 cut(s) 209, 801, 818
BstV1I GCAGC 6 cut(s) 254, 390, 443, 500, 508, 870
BstZI CGGCCG 2 cut(s) 241, 789
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 4 cut(s) 161, 243, 791, 872
BtgI CCRYGG 2 cut(s) 207, 873
BtgZI GCGATG 1 cut(s) 900
BtrI CACGTC 1 cut(s) 744
BtsCI GGATG 2 cut(s) 153, 620
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 6 cut(s) 238, 454, 728, 801, 818, 870
CfoI GCGC 4 cut(s) 801, 803, 818, 820
Cfr10I RCCGGY 4 cut(s) 80, 97, 104, 261
Cfr13I GGNCC 2 cut(s) 160, 850
Cfr42I CCGCGG 1 cut(s) 210
CseI GACGC 3 cut(s) 382, 470, 954
Csp6I GTAC 3 cut(s) 607, 772, 811
CspAI ACCGGT 2 cut(s) 97, 104
CviAII CATG 2 cut(s) 237, 862
CviQI GTAC 3 cut(s) 607, 772, 811
DdeI CTNAG 2 cut(s) 441, 509
DpnI GATC 4 cut(s) 310, 500, 890, 956
DpnII GATC 4 cut(s) 308, 498, 888, 954
DraIII CACNNNGTG 1 cut(s) 624
EaeI YGGCCR 3 cut(s) 241, 789, 870
EagI CGGCCG 2 cut(s) 241, 789
Eam1104I CTCTTC 3 cut(s) 221, 296, 607
EarI CTCTTC 3 cut(s) 221, 296, 607
Ecl136II GAGCTC 3 cut(s) 214, 322, 635
EclXI CGGCCG 2 cut(s) 241, 789
Eco130I CCWWGG 1 cut(s) 628
Eco24I GRGCYC 3 cut(s) 216, 324, 637
Eco31I GGTCTC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 850
Eco52I CGGCCG 2 cut(s) 241, 789
Eco53kI GAGCTC 3 cut(s) 214, 322, 635
Eco72I CACGTG 1 cut(s) 840
Eco88I CYCGRG 3 cut(s) 317, 537, 581
Eco91I GGTNACC 2 cut(s) 100, 107
EcoICRI GAGCTC 3 cut(s) 214, 322, 635
EcoO65I GGTNACC 2 cut(s) 100, 107
EcoRII CCWGG 2 cut(s) 284, 852
EcoT14I CCWWGG 1 cut(s) 628
EcoT38I GRGCYC 3 cut(s) 216, 324, 637
ErhI CCWWGG 1 cut(s) 628
Esp3I CGTCTC 1 cut(s) 972
FaeI CATG 2 cut(s) 240, 865
FaiI YATR 3 cut(s) 238, 806, 863
FaqI GGGAC 1 cut(s) 154
FatI CATG 2 cut(s) 236, 861
FauI CCCGC 3 cut(s) 526, 667, 831
FbaI TGATCA 1 cut(s) 954
FblI GTMKAC 1 cut(s) 85
FokI GGATG 2 cut(s) 140, 627
FriOI GRGCYC 3 cut(s) 216, 324, 637
GlaI GCGC 4 cut(s) 800, 802, 817, 819
GsaI CCCAGC 1 cut(s) 118
GsuI CTGGAG 2 cut(s) 124, 623
HaeIII GGCC 4 cut(s) 161, 243, 791, 872
HgaI GACGC 3 cut(s) 382, 470, 954
HhaI GCGC 4 cut(s) 801, 803, 818, 820
Hin1I GRCGYC 2 cut(s) 462, 965
Hin1II CATG 2 cut(s) 240, 865
Hin6I GCGC 4 cut(s) 799, 801, 816, 818
HinP1I GCGC 4 cut(s) 799, 801, 816, 818
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 6 cut(s) 380, 436, 483, 577, 878, 943
HphI GGTGA 2 cut(s) 94, 101
Hpy166II GTNNAC 2 cut(s) 86, 397
Hpy188I TCNGA 4 cut(s) 334, 435, 780, 954
Hpy188III TCNNGA 9 cut(s) 537, 574, 581, 583, 612, 640, 830, 892, 931
Hpy8I GTNNAC 2 cut(s) 86, 397
Hpy99I CGWCG 2 cut(s) 334, 464
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 784
HpyCH4IV ACGT 3 cut(s) 531, 743, 839
HpyCH4V TGCA 3 cut(s) 406, 618, 886
HpyF3I CTNAG 2 cut(s) 441, 509
HpySE526I ACGT 3 cut(s) 531, 743, 839
Hsp92I GRCGYC 2 cut(s) 462, 965
Hsp92II CATG 2 cut(s) 240, 865
HspAI GCGC 4 cut(s) 799, 801, 816, 818
Ksp22I TGATCA 1 cut(s) 954
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 4 cut(s) 308, 498, 888, 954
LguI GCTCTTC 2 cut(s) 221, 296
Lsp1109I GCAGC 6 cut(s) 254, 390, 443, 500, 508, 870
LweI GCATC 4 cut(s) 360, 605, 835, 923
MaeII ACGT 3 cut(s) 531, 743, 839
MaeIII GTNAC 3 cut(s) 100, 107, 936
MalI GATC 4 cut(s) 310, 500, 890, 956
MbiI CCGCTC 2 cut(s) 26, 121
MboI GATC 4 cut(s) 308, 498, 888, 954
MboII GAAGA 6 cut(s) 5, 28, 208, 283, 594, 598
MhlI GDGCHC 5 cut(s) 216, 324, 357, 637, 689
MlyI GAGTC 1 cut(s) 374
MmeI TCCRAC 2 cut(s) 312, 803
MseI TTAA 1 cut(s) 53
MslI CAYNNNNRTG 1 cut(s) 932
MspA1I CMGCKG 4 cut(s) 209, 456, 521, 868
MspR9I CCNGG 6 cut(s) 129, 158, 286, 757, 848, 854
MvaI CCWGG 2 cut(s) 286, 854
MvnI CGCG 3 cut(s) 209, 801, 818
NciI CCSGG 4 cut(s) 129, 158, 757, 848
NdeII GATC 4 cut(s) 308, 498, 888, 954
NlaIII CATG 2 cut(s) 240, 865
NlaIV GGNNCC 6 cut(s) 260, 342, 354, 491, 505, 749
NmeAIII GCCGAG 2 cut(s) 269, 326
NmuCI GTSAC 2 cut(s) 100, 107
NspI RCATGY 2 cut(s) 240, 865
PaeI GCATGC 1 cut(s) 240
PaeR7I CTCGAG 3 cut(s) 317, 537, 581
PauI GCGCGC 2 cut(s) 799, 816
PciSI GCTCTTC 2 cut(s) 221, 296
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 5 cut(s) 436, 483, 577, 878, 943
PflMI CCANNNNNTGG 1 cut(s) 733
PinAI ACCGGT 2 cut(s) 97, 104
PleI GAGTC 1 cut(s) 374
PmaCI CACGTG 1 cut(s) 840
PmlI CACGTG 1 cut(s) 840
PpsI GAGTC 1 cut(s) 374
Ppu21I YACGTR 1 cut(s) 840
PshBI ATTAAT 1 cut(s) 53
Psp124BI GAGCTC 3 cut(s) 216, 324, 637
Psp6I CCWGG 2 cut(s) 284, 852
PspCI CACGTG 1 cut(s) 840
PspEI GGTNACC 2 cut(s) 100, 107
PspFI CCCAGC 1 cut(s) 114
PspGI CCWGG 2 cut(s) 284, 852
PspN4I GGNNCC 6 cut(s) 260, 342, 354, 491, 505, 749
PspPI GGNCC 2 cut(s) 160, 850
PspXI VCTCGAGB 1 cut(s) 317
PstI CTGCAG 2 cut(s) 408, 888
PteI GCGCGC 2 cut(s) 799, 816
PvuII CAGCTG 1 cut(s) 456
RsaI GTAC 3 cut(s) 608, 773, 812
RsaNI GTAC 3 cut(s) 607, 772, 811
RseI CAYNNNNRTG 1 cut(s) 932
SacI GAGCTC 3 cut(s) 216, 324, 637
SacII CCGCGG 1 cut(s) 210
SapI GCTCTTC 2 cut(s) 221, 296
SaqAI TTAA 1 cut(s) 53
Sau3AI GATC 4 cut(s) 308, 498, 888, 954
Sau96I GGNCC 2 cut(s) 160, 850
SchI GAGTC 1 cut(s) 374
ScrFI CCNGG 6 cut(s) 129, 158, 286, 757, 848, 854
SduI GDGCHC 5 cut(s) 216, 324, 357, 637, 689
SfaNI GCATC 4 cut(s) 360, 605, 835, 923
SfcI CTRYAG 2 cut(s) 404, 884
Sfr274I CTCGAG 3 cut(s) 317, 537, 581
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SinI GGWCC 1 cut(s) 850
SlaI CTCGAG 3 cut(s) 317, 537, 581
SmiMI CAYNNNNRTG 1 cut(s) 932
SmlI CTYRAG 4 cut(s) 317, 537, 581, 647
SmoI CTYRAG 4 cut(s) 317, 537, 581, 647
SphI GCATGC 1 cut(s) 240
SstI GAGCTC 3 cut(s) 216, 324, 637
StyD4I CCNGG 6 cut(s) 127, 156, 284, 755, 846, 852
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 1 cut(s) 784
TaiI ACGT 3 cut(s) 534, 746, 842
TaqI TCGA 5 cut(s) 252, 318, 538, 582, 891
TatI WGTACW 1 cut(s) 810
TauI GCSGC 9 cut(s) 121, 243, 284, 299, 569, 597, 868, 914, 922
TfiI GAWTC 5 cut(s) 436, 483, 577, 878, 943
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TseFI GTSAC 2 cut(s) 100, 107
TseI GCWGC 6 cut(s) 267, 403, 456, 513, 521, 883
Tsp45I GTSAC 2 cut(s) 100, 107
TspGWI ACGGA 1 cut(s) 681
Van91I CCANNNNNTGG 1 cut(s) 733
VpaK11BI GGWCC 1 cut(s) 850
VspI ATTAAT 1 cut(s) 53
XceI RCATGY 2 cut(s) 240, 865
XhoI CTCGAG 3 cut(s) 317, 537, 581
XmiI GTMKAC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.