AT4G28780

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012747
Physical Location & Seq
Forward (+)
14215473 .. 14217909
2437 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28780.1

Sequence Viewer

Length: 1104 bp
ATGTCCACATTTCTCCTAACATGGATTATTATGACCGTCGCGTTAAGCGTAACGCTTTTTCTTATGCCGCAACAAACAAATGCGGCTAGAGCGTTCTTCGTGTTTGGCGACTCACTCGTGGACAGTGGTAATAACAATTACTTAGTCACCACTGCTCGTGCCGATTCTCCACCGTATGGAATCGATTATCCGACTGGTCGACCAACCGGTCGTTTCTCCAACGGCTTGAACCTCCCTGACATCATCAGTGAACAAATTGGATCTGAGCCTACATTACCAATTCTTAGCCCTGAACTCACTGGAGAGAAGCTATTGATCGGAGCTAACTTTGCCTCTGCCGGCATTGGAATTCTCAACGATACTGGCGTTCAATTTTTGAACATACTAAGGATCGGTAGGCAATTCGAGCTGTTCCAAGAGTATCAAGAGAGAGTGAGTGAGATTATAGGTTCAGACAAAACGCAACAGCTTGTAAATGGGGCTCTCGTCCTCATGACTCTCGGCGGCAATGATTTCGTCAACAACTACTTCTTCCCCATCTCTACTAGAAGACGCCAATCATCTCTCGGTGAATTTAGCCAGCTTCTCATCTCCGAATACAAGAAAATCCTGACGAGTCTTTATGAGTTGGGAGCAAGGAGGGTGATGGTGACGGGAACAGGACCTTTGGGTTGCGTACCAGCGGAGCTTGCCTCATCGGGAAGTGTGAATGGAGAATGTGCACCCGAGGCGCAACAGGCTGCAGCAATCTTCAATCCTCTTTTAGTTCAAATGCTTCAAGGACTTAACCGTGAGATTGGTTCTGATGTTTTCATCGGGGCTAATGCCTTTAACACGAATGCGGATTTCATCAACAATCCCCAAAGATTTGGTTTTGTGACGTCAAAAGTAGCATGTTGTGGACAAGGAGCATACAACGGGCAAGGCGTTTGCACCCCATTGTCGACTTTATGCTCTGACCGCAATGCCTATGCTTTCTGGGACCCTTTCCACCCGACCGAGAAAGCCACCCGACTCATCGTCCAACAAATCATGACTGGTTCGGTTGAGTACATGAACCCCATGAACCTTAGTACTATTATGGCTTTAGACTCCAGAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

39.9

Weight (kDa)

4.9

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 343 2.9e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 207
AatII GACGTC 1 cut(s) 884
AccB7I CCANNNNNTGG 1 cut(s) 176
AccI GTMKAC 2 cut(s) 199, 944
AccII CGCG 1 cut(s) 41
AciI CCGC 6 cut(s) 68, 83, 504, 683, 842, 961
AclWI GGATC 2 cut(s) 268, 398
AcsI RAATTY 2 cut(s) 348, 572
AcyI GRCGYC 2 cut(s) 553, 881
AfaI GTAC 3 cut(s) 678, 1052, 1075
AfiI CCNNNNNNNGG 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 206
AgsI TTSAA 6 cut(s) 229, 371, 379, 754, 770, 779
AhdI GACNNNNNGTC 1 cut(s) 1019
AluBI AGCT 6 cut(s) 310, 323, 409, 469, 583, 688
AluI AGCT 6 cut(s) 310, 323, 409, 469, 583, 688
Alw21I GWGCWC 1 cut(s) 724
Alw44I GTGCAC 1 cut(s) 720
AlwI GGATC 2 cut(s) 268, 398
Ama87I CYCGRG 1 cut(s) 725
ApaLI GTGCAC 1 cut(s) 720
ApeKI GCWGC 2 cut(s) 740, 743
ApoI RAATTY 2 cut(s) 348, 572
AsiGI ACCGGT 1 cut(s) 206
AspLEI GCGC 1 cut(s) 733
AspS9I GGNCC 2 cut(s) 662, 982
AsuHPI GGTGA 4 cut(s) 139, 581, 655, 661
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 2 cut(s) 662, 982
BaeGI GKGCMC 1 cut(s) 724
BaeI ACNNNNGTAYC 2 cut(s) 351, 384
BanII GRGCYC 1 cut(s) 484
BauI CACGAG 2 cut(s) 116, 156
BbsI GAAGAC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 724
BbvI GCAGC 2 cut(s) 727, 755
BccI CCATC 2 cut(s) 545, 640
BceAI ACGGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BfaI CTAG 2 cut(s) 87, 546
BfmI CTRYAG 1 cut(s) 741
BisI GCNGC 5 cut(s) 68, 84, 505, 741, 744
BlsI GCNGC 5 cut(s) 69, 85, 506, 742, 745
BmcAI AGTACT 1 cut(s) 1075
Bme18I GGWCC 2 cut(s) 662, 982
BmeRI GACNNNNNGTC 1 cut(s) 1019
BmeT110I CYCGRG 1 cut(s) 725
BmgT120I GGNCC 2 cut(s) 662, 982
BmiI GGNNCC 2 cut(s) 983, 984
BpiI GAAGAC 1 cut(s) 556
BplI GAGNNNNNCTC 2 cut(s) 677, 709
BpmI CTGGAG 2 cut(s) 321, 1078
Bsa29I ATCGAT 1 cut(s) 183
BsaHI GRCGYC 2 cut(s) 553, 881
BsaJI CCNNGG 1 cut(s) 726
BsaWI WCCGGW 1 cut(s) 206
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse118I RCCGGY 2 cut(s) 206, 338
Bse1I ACTGG 4 cut(s) 199, 304, 367, 1042
Bse3DI GCAATG 2 cut(s) 514, 970
BseCI ATCGAT 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 726
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 2 cut(s) 514, 970
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 4 cut(s) 199, 304, 367, 1042
BseSI GKGCMC 1 cut(s) 724
BseXI GCAGC 2 cut(s) 727, 755
Bsh1236I CGCG 1 cut(s) 41
Bsh1285I CGRYCG 2 cut(s) 211, 999
BshTI ACCGGT 1 cut(s) 206
BshVI ATCGAT 1 cut(s) 183
BsiEI CGRYCG 2 cut(s) 211, 999
BsiHKAI GWGCWC 1 cut(s) 724
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 2 cut(s) 207, 339
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 995
BsmI GAATGC 1 cut(s) 844
BsoBI CYCGRG 1 cut(s) 725
Bsp1286I GDGCHC 2 cut(s) 484, 724
Bsp143I GATC 3 cut(s) 260, 315, 390
BspACI CCGC 6 cut(s) 68, 83, 504, 683, 842, 961
BspCNI CTCAG 1 cut(s) 256
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 41
BspHI TCATGA 2 cut(s) 492, 1032
BspLI GGNNCC 2 cut(s) 983, 984
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 2 cut(s) 268, 398
BsrDI GCAATG 2 cut(s) 514, 970
BsrFI RCCGGY 2 cut(s) 206, 338
BsrI ACTGG 4 cut(s) 199, 304, 367, 1042
BssAI RCCGGY 2 cut(s) 206, 338
BssECI CCNNGG 1 cut(s) 726
BssMI GATC 3 cut(s) 260, 315, 390
BssNI GRCGYC 2 cut(s) 553, 881
BssSI CACGAG 2 cut(s) 116, 156
Bst2BI CACGAG 2 cut(s) 116, 156
Bst4CI ACNGT 4 cut(s) 37, 125, 174, 791
BstACI GRCGYC 2 cut(s) 553, 881
BstC8I GCNNGC 3 cut(s) 340, 581, 690
BstDEI CTNAG 5 cut(s) 142, 264, 284, 386, 1070
BstFNI CGCG 1 cut(s) 41
BstHHI GCGC 1 cut(s) 733
BstKTI GATC 3 cut(s) 263, 318, 393
BstMBI GATC 3 cut(s) 260, 315, 390
BstMCI CGRYCG 2 cut(s) 211, 999
BstMWI GCNNNNNNNGC 7 cut(s) 89, 329, 406, 689, 728, 737, 960
BstNSI RCATGY 1 cut(s) 897
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 1 cut(s) 724
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 2 cut(s) 727, 755
BstV2I GAAGAC 1 cut(s) 556
BstX2I RGATCY 1 cut(s) 260
BstXI CCANNNNNNTGG 1 cut(s) 869
BstYI RGATCY 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 4 cut(s) 130, 150, 253, 297
Cac8I GCNNGC 3 cut(s) 340, 581, 690
CciI TCATGA 2 cut(s) 492, 1032
CfoI GCGC 1 cut(s) 733
Cfr10I RCCGGY 2 cut(s) 206, 338
Cfr13I GGNCC 2 cut(s) 662, 982
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 561
Csp6I GTAC 3 cut(s) 677, 1051, 1074
CspAI ACCGGT 1 cut(s) 206
CviAII CATG 6 cut(s) 21, 493, 894, 1033, 1054, 1063
CviQI GTAC 3 cut(s) 677, 1051, 1074
DdeI CTNAG 5 cut(s) 142, 264, 284, 386, 1070
DpnI GATC 3 cut(s) 262, 317, 392
DpnII GATC 3 cut(s) 260, 315, 390
DrdI GACNNNNNNGTC 1 cut(s) 207
DriI GACNNNNNGTC 1 cut(s) 1019
DseDI GACNNNNNNGTC 1 cut(s) 207
Eam1105I GACNNNNNGTC 1 cut(s) 1019
Eco24I GRGCYC 1 cut(s) 484
Eco47I GGWCC 2 cut(s) 662, 982
Eco88I CYCGRG 1 cut(s) 725
EcoO109I RGGNCCY 2 cut(s) 662, 982
EcoRI GAATTC 1 cut(s) 348
EcoT38I GRGCYC 1 cut(s) 484
FaeI CATG 6 cut(s) 24, 496, 897, 1036, 1057, 1066
FaqI GGGAC 1 cut(s) 995
FatI CATG 6 cut(s) 20, 492, 893, 1032, 1053, 1062
FblI GTMKAC 2 cut(s) 199, 944
Fnu4HI GCNGC 5 cut(s) 68, 84, 505, 741, 744
FriOI GRGCYC 1 cut(s) 484
Fsp4HI GCNGC 5 cut(s) 68, 84, 505, 741, 744
FspBI CTAG 2 cut(s) 87, 546
GlaI GCGC 1 cut(s) 732
GluI GCNGC 5 cut(s) 68, 84, 505, 741, 744
GsuI CTGGAG 2 cut(s) 321, 1078
HapII CCGG 2 cut(s) 207, 339
HgaI GACGC 1 cut(s) 561
HhaI GCGC 1 cut(s) 733
Hin1I GRCGYC 2 cut(s) 553, 881
Hin1II CATG 6 cut(s) 24, 496, 897, 1036, 1057, 1066
Hin6I GCGC 1 cut(s) 731
HinP1I GCGC 1 cut(s) 731
HincII GTYRAC 3 cut(s) 200, 520, 945
HindII GTYRAC 3 cut(s) 200, 520, 945
HinfI GANTC 7 cut(s) 110, 164, 180, 496, 616, 1014, 1091
HpaII CCGG 2 cut(s) 207, 339
HphI GGTGA 4 cut(s) 139, 581, 655, 661
Hpy166II GTNNAC 8 cut(s) 6, 121, 200, 251, 520, 722, 902, 945
Hpy188I TCNGA 7 cut(s) 192, 265, 320, 454, 595, 805, 958
Hpy188III TCNNGA 6 cut(s) 425, 493, 610, 699, 1033, 1095
Hpy8I GTNNAC 8 cut(s) 6, 121, 200, 251, 520, 722, 902, 945
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 4 cut(s) 37, 125, 174, 791
HpyCH4IV ACGT 1 cut(s) 881
HpyCH4V TGCA 3 cut(s) 722, 743, 933
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 329, 406, 689, 728, 737, 960
HpyF3I CTNAG 5 cut(s) 142, 264, 284, 386, 1070
HpySE526I ACGT 1 cut(s) 881
Hsp92I GRCGYC 2 cut(s) 553, 881
Hsp92II CATG 6 cut(s) 24, 496, 897, 1036, 1057, 1066
HspAI GCGC 1 cut(s) 731
KflI GGGWCCC 1 cut(s) 982
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
Kzo9I GATC 3 cut(s) 260, 315, 390
LmnI GCTCC 4 cut(s) 320, 632, 685, 908
Lsp1109I GCAGC 2 cut(s) 727, 755
MaeI CTAG 2 cut(s) 87, 546
MaeII ACGT 1 cut(s) 881
MaeIII GTNAC 4 cut(s) 49, 145, 649, 877
MalI GATC 3 cut(s) 262, 317, 392
MboI GATC 3 cut(s) 260, 315, 390
MboII GAAGA 4 cut(s) 88, 523, 561, 742
MflI RGATCY 1 cut(s) 260
MhlI GDGCHC 2 cut(s) 484, 724
MluCI AATT 7 cut(s) 136, 255, 279, 348, 371, 401, 572
MlyI GAGTC 5 cut(s) 104, 490, 625, 1008, 1085
MmeI TCCRAC 3 cut(s) 215, 243, 1048
MnlI CCTC 7 cut(s) 242, 343, 500, 633, 703, 721, 768
MroNI GCCGGC 1 cut(s) 338
MseI TTAA 3 cut(s) 44, 786, 831
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 2 cut(s) 207, 339
Mva1269I GAATGC 1 cut(s) 844
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 7 cut(s) 89, 329, 406, 689, 728, 737, 960
NaeI GCCGGC 1 cut(s) 340
NdeII GATC 3 cut(s) 260, 315, 390
NgoMIV GCCGGC 1 cut(s) 338
NlaIII CATG 6 cut(s) 24, 496, 897, 1036, 1057, 1066
NlaIV GGNNCC 2 cut(s) 983, 984
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 3 cut(s) 145, 649, 877
NspI RCATGY 1 cut(s) 897
PagI TCATGA 2 cut(s) 492, 1032
PcsI WCGNNNNNNNCGW 4 cut(s) 45, 105, 363, 924
PctI GAATGC 1 cut(s) 844
PdiI GCCGGC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 164, 180
PflMI CCANNNNNTGG 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 206
PkrI GCNGC 5 cut(s) 69, 85, 506, 742, 745
PleI GAGTC 5 cut(s) 104, 490, 624, 1008, 1085
PpsI GAGTC 5 cut(s) 104, 490, 624, 1008, 1085
PpuMI RGGWCCY 2 cut(s) 662, 982
Psp5II RGGWCCY 2 cut(s) 662, 982
PspN4I GGNNCC 2 cut(s) 983, 984
PspPI GGNCC 2 cut(s) 662, 982
PspPPI RGGWCCY 2 cut(s) 662, 982
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 260
RsaI GTAC 3 cut(s) 678, 1052, 1075
RsaNI GTAC 3 cut(s) 677, 1051, 1074
SalI GTCGAC 2 cut(s) 198, 943
SaqAI TTAA 3 cut(s) 44, 786, 831
SatI GCNGC 5 cut(s) 68, 84, 505, 741, 744
Sau3AI GATC 3 cut(s) 260, 315, 390
Sau96I GGNCC 2 cut(s) 662, 982
ScaI AGTACT 1 cut(s) 1075
SchI GAGTC 5 cut(s) 104, 490, 625, 1008, 1085
SduI GDGCHC 2 cut(s) 484, 724
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 2 cut(s) 662, 982
Sse9I AATT 7 cut(s) 136, 255, 279, 348, 371, 401, 572
SsiI CCGC 6 cut(s) 68, 83, 504, 683, 842, 961
SspMI CTAG 2 cut(s) 87, 546
TaaI ACNGT 4 cut(s) 37, 125, 174, 791
TaiI ACGT 1 cut(s) 884
TaqI TCGA 4 cut(s) 183, 199, 405, 944
TaqII GACCGA 1 cut(s) 1013
TasI AATT 7 cut(s) 136, 255, 279, 348, 371, 401, 572
TatI WGTACW 2 cut(s) 1050, 1073
TauI GCSGC 3 cut(s) 70, 86, 507
TfiI GAWTC 2 cut(s) 164, 180
Tru1I TTAA 3 cut(s) 44, 786, 831
Tru9I TTAA 3 cut(s) 44, 786, 831
TscAI CASTG 4 cut(s) 130, 157, 253, 304
TseFI GTSAC 3 cut(s) 145, 649, 877
TseI GCWGC 2 cut(s) 740, 743
Tsp45I GTSAC 3 cut(s) 145, 649, 877
TspDTI ATGAA 4 cut(s) 802, 838, 1070, 1079
TspRI CASTG 4 cut(s) 130, 157, 253, 304
Van91I CCANNNNNTGG 1 cut(s) 176
VneI GTGCAC 1 cut(s) 720
VpaK11BI GGWCC 2 cut(s) 662, 982
XapI RAATTY 2 cut(s) 348, 572
XceI RCATGY 1 cut(s) 897
XmiI GTMKAC 2 cut(s) 199, 944
XspI CTAG 2 cut(s) 87, 546
ZraI GACGTC 1 cut(s) 882
ZrmI AGTACT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.