Rw6G024690

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012747
Physical Location & Seq
Reverse (-)
47854399 .. 47856105
1707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G024690.1

Sequence Viewer

Length: 1089 bp
ATGGCTGTTGCCCTAGTGCTAATGGTGGCAATGAGCACGGTGATTCTCGCACCTCATCAAGCTGAGGCAGCTCGCGCTTTCTTTGTTTTCGGAGACTCCCTTGTGGATAACGGCAACAACAATTACTTGGCCACCACCGCGCGTGCCGATTTTCCTCCCTACGGCATTGATTTCCCTACCCACCGCCCCACCGGCCGCTTCTCCAACGGCTTAAACCTCCCTGATCTCATCAGCGAGCAAATAGGATCGGAGCTAGTCTTGCCATATTTGAGCAGTGAGCTCACCGGACAAAAGCTGCTTATTGGTGCTAACTTTGCTTCTGCTGGAATTGGAATCCTCAATGATACCGGTGTTCAATTTATCAACATAATAAGGATCTTTAGGCAGTTTGAGATGTTCCAGCAATACCAGCAACGAGTGAGTGCCCTAATCGGAGCAGCGCAAGCCAAGAGGCTGGTAAACGACGCTCTTGTCCTCATAACTCTCGGCGGCAATGACTTCGTCAACAACTACTTCTTGGTACCCTTCTCTGCAAGATCACTCCAGTACTCAATCCCTCAGTACTCCCAGTACCTCATCTCCGAGTACCGGAAAATTCTCCTGAGACTATTTGAATTAGGAGGGAGAAGGGTGCTGGTGACCGGTACAGGCCCATTAGGCTGTGTTCCCGCCGCTATGGCCCAGAGGAGTAGGAATGGTGAATGTGCACCAGAGCTCCAGAGGGCAGCAGCCATCTTCAATCCCCTGCTTGTCCAGATGGTTCAGGGACTCAACGCTCAGCTTGATACTGACGCTTTCATTGCTGCTAATGCGTTTGCAAATGTGGAGTTCCTCAGTAACCCCCGGAAATATGGCTTTGTTACGTCAAAGGTGGCATGTTGCGGGCAAGGACCGTACAATGGGTTGGGACTATGTACGCCATTGTCGAATCTGTGCTCCAACCGGGATGTGTATGCGTTTTGGGATCCTTTCCATCCATCTGAGCGGGCGAACCGGCTGATTGTGCAGACGATCATGACCGGGTCTAGCAAGTACATGAGCCCTATGAACCTAAGCACCATCATGGCCATGGACTCCGGGAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.54

Weight (kDa)

8.18

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 336 2.5e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 470
Acc65I GGTACC 1 cut(s) 520
AccB1I GGYRCC 1 cut(s) 520
AccBSI CCGCTC 1 cut(s) 985
AccII CGCG 3 cut(s) 75, 140, 142
AciI CCGC 8 cut(s) 138, 184, 196, 489, 669, 672, 882, 985
AclWI GGATC 4 cut(s) 253, 383, 959, 972
AcoI YGGCCR 3 cut(s) 129, 193, 1065
AcsI RAATTY 1 cut(s) 594
AfaI GTAC 9 cut(s) 522, 548, 563, 572, 587, 646, 896, 916, 1034
AfiI CCNNNNNNNGG 3 cut(s) 161, 588, 899
AgeI ACCGGT 2 cut(s) 347, 641
AgsI TTSAA 3 cut(s) 356, 614, 739
AluBI AGCT 7 cut(s) 62, 71, 253, 280, 295, 715, 781
AluI AGCT 7 cut(s) 62, 71, 253, 280, 295, 715, 781
Alw21I GWGCWC 5 cut(s) 38, 282, 709, 717, 938
Alw26I GTCTC 2 cut(s) 87, 598
Alw44I GTGCAC 1 cut(s) 705
AlwI GGATC 4 cut(s) 253, 383, 959, 972
AoxI GGCC 5 cut(s) 129, 193, 649, 678, 1065
ApaLI GTGCAC 1 cut(s) 705
ApeKI GCWGC 6 cut(s) 68, 295, 437, 725, 728, 803
ApoI RAATTY 1 cut(s) 594
AsiGI ACCGGT 2 cut(s) 347, 641
Asp718I GGTACC 1 cut(s) 520
AspLEI GCGC 3 cut(s) 77, 142, 442
AspS9I GGNCC 3 cut(s) 650, 679, 890
AsuC2I CCSGG 4 cut(s) 844, 944, 1021, 1078
AsuHPI GGTGA 4 cut(s) 52, 274, 649, 710
AvaII GGWCC 1 cut(s) 890
BaeGI GKGCMC 2 cut(s) 427, 709
BaeI ACNNNNGTAYC 2 cut(s) 336, 369
BalI TGGCCA 2 cut(s) 131, 1067
BamHI GGATCC 1 cut(s) 964
BanI GGYRCC 1 cut(s) 520
BanII GRGCYC 3 cut(s) 282, 717, 1043
Bbv12I GWGCWC 5 cut(s) 38, 282, 709, 717, 938
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 6 cut(s) 80, 282, 449, 737, 740, 790
BccI CCATC 5 cut(s) 740, 751, 981, 985, 1067
BceAI ACGGC 3 cut(s) 127, 178, 223
BcgI CGANNNNNNTGC 2 cut(s) 481, 515
BcnI CCSGG 4 cut(s) 844, 944, 1021, 1078
BcoDI GTCTC 2 cut(s) 87, 598
BfaI CTAG 4 cut(s) 14, 254, 1026, 1087
BglI GCCNNNNNGGC 3 cut(s) 192, 657, 677
BisI GCNGC 9 cut(s) 69, 196, 296, 438, 490, 672, 726, 729, 804
BlpI GCTNAGC 1 cut(s) 777
BlsI GCNGC 9 cut(s) 70, 197, 297, 439, 491, 673, 727, 730, 805
BmcAI AGTACT 2 cut(s) 548, 563
Bme1390I CCNGG 4 cut(s) 844, 944, 1021, 1078
Bme18I GGWCC 1 cut(s) 890
BmgT120I GGNCC 3 cut(s) 650, 679, 890
BmiI GGNNCC 2 cut(s) 522, 966
BmrFI CCNGG 4 cut(s) 844, 944, 1021, 1078
BmrI ACTGGG 1 cut(s) 562
BmuI ACTGGG 1 cut(s) 562
BpmI CTGGAG 2 cut(s) 527, 701
Bpu10I CCTNAGC 2 cut(s) 63, 1052
Bpu1102I GCTNAGC 1 cut(s) 777
BpuMI CCSGG 4 cut(s) 844, 944, 1021, 1078
BsaJI CCNNGG 2 cut(s) 842, 1068
BsaWI WCCGGW 4 cut(s) 284, 347, 588, 641
BsaXI ACNNNNNCTCC 4 cut(s) 563, 593, 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 588, 899
Bse118I RCCGGY 4 cut(s) 191, 347, 641, 993
Bse1I ACTGG 2 cut(s) 544, 568
Bse3DI GCAATG 3 cut(s) 36, 499, 798
BseDI CCNNGG 2 cut(s) 842, 1068
BseGI GGATG 2 cut(s) 952, 973
BseLI CCNNNNNNNGG 3 cut(s) 161, 588, 899
BseMI GCAATG 3 cut(s) 36, 499, 798
BseMII CTCAG 6 cut(s) 54, 572, 593, 791, 847, 972
BseNI ACTGG 2 cut(s) 544, 568
BseRI GAGGAG 1 cut(s) 700
BseSI GKGCMC 2 cut(s) 427, 709
BseX3I CGGCCG 1 cut(s) 193
BseXI GCAGC 6 cut(s) 80, 282, 449, 737, 740, 790
BsgI GTGCAG 1 cut(s) 1025
Bsh1236I CGCG 3 cut(s) 75, 140, 142
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 5 cut(s) 131, 195, 651, 680, 1067
BshNI GGYRCC 1 cut(s) 520
BshTI ACCGGT 2 cut(s) 347, 641
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 5 cut(s) 38, 282, 709, 717, 938
BslFI GGGAC 2 cut(s) 780, 921
BslI CCNNNNNNNGG 3 cut(s) 161, 588, 899
BsmAI GTCTC 2 cut(s) 87, 598
BsmFI GGGAC 2 cut(s) 780, 921
BsnI GGCC 5 cut(s) 131, 195, 651, 680, 1067
Bsp1286I GDGCHC 7 cut(s) 38, 282, 427, 709, 717, 938, 1043
Bsp143I GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
Bsp1720I GCTNAGC 1 cut(s) 777
Bsp19I CCATGG 1 cut(s) 1068
BspACI CCGC 8 cut(s) 138, 184, 196, 489, 669, 672, 882, 985
BspANI GGCC 5 cut(s) 131, 195, 651, 680, 1067
BspCNI CTCAG 6 cut(s) 55, 571, 594, 790, 846, 973
BspFNI CGCG 3 cut(s) 75, 140, 142
BspHI TCATGA 1 cut(s) 1014
BspLI GGNNCC 2 cut(s) 522, 966
BspPI GGATC 4 cut(s) 253, 383, 959, 972
BspT107I GGYRCC 1 cut(s) 520
BsrBI CCGCTC 1 cut(s) 985
BsrDI GCAATG 3 cut(s) 36, 499, 798
BsrFI RCCGGY 4 cut(s) 191, 347, 641, 993
BsrI ACTGG 2 cut(s) 544, 568
BssAI RCCGGY 4 cut(s) 191, 347, 641, 993
BssECI CCNNGG 2 cut(s) 842, 1068
BssMI GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
BssT1I CCWWGG 1 cut(s) 1068
Bst4CI ACNGT 2 cut(s) 40, 894
BstC8I GCNNGC 6 cut(s) 73, 144, 236, 444, 884, 987
BstDEI CTNAG 7 cut(s) 63, 558, 602, 777, 833, 981, 1052
BstDSI CCRYGG 1 cut(s) 1068
BstEII GGTNACC 1 cut(s) 637
BstF5I GGATG 2 cut(s) 952, 973
BstFNI CGCG 3 cut(s) 75, 140, 142
BstHHI GCGC 3 cut(s) 77, 142, 442
BstKTI GATC 6 cut(s) 226, 248, 378, 539, 967, 1014
BstMAI GTCTC 2 cut(s) 87, 598
BstMBI GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
BstMCI CGRYCG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 879
BstPI GGTNACC 1 cut(s) 637
BstSCI CCNGG 4 cut(s) 842, 942, 1019, 1076
BstSLI GKGCMC 2 cut(s) 427, 709
BstUI CGCG 3 cut(s) 75, 140, 142
BstV1I GCAGC 6 cut(s) 80, 282, 449, 737, 740, 790
BstX2I RGATCY 2 cut(s) 375, 964
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 2 cut(s) 375, 964
BstZI CGGCCG 1 cut(s) 193
BsuRI GGCC 5 cut(s) 131, 195, 651, 680, 1067
BtgI CCRYGG 1 cut(s) 1068
BtsCI GGATG 2 cut(s) 952, 973
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 1 cut(s) 280
Cac8I GCNNGC 6 cut(s) 73, 144, 236, 444, 884, 987
CciI TCATGA 1 cut(s) 1014
CfoI GCGC 3 cut(s) 77, 142, 442
Cfr10I RCCGGY 4 cut(s) 191, 347, 641, 993
Cfr13I GGNCC 3 cut(s) 650, 679, 890
CseI GACGC 2 cut(s) 473, 800
Csp6I GTAC 9 cut(s) 521, 547, 562, 571, 586, 645, 895, 915, 1033
CspAI ACCGGT 2 cut(s) 347, 641
CviAII CATG 5 cut(s) 876, 1015, 1036, 1063, 1069
CviQI GTAC 9 cut(s) 521, 547, 562, 571, 586, 645, 895, 915, 1033
DdeI CTNAG 7 cut(s) 63, 558, 602, 777, 833, 981, 1052
DpnI GATC 6 cut(s) 225, 247, 377, 538, 966, 1013
DpnII GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
DrdI GACNNNNNNGTC 1 cut(s) 470
DseDI GACNNNNNNGTC 1 cut(s) 470
EaeI YGGCCR 3 cut(s) 129, 193, 1065
EagI CGGCCG 1 cut(s) 193
Ecl136II GAGCTC 2 cut(s) 280, 715
EclXI CGGCCG 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 1068
Eco24I GRGCYC 3 cut(s) 282, 717, 1043
Eco47I GGWCC 1 cut(s) 890
Eco52I CGGCCG 1 cut(s) 193
Eco53kI GAGCTC 2 cut(s) 280, 715
Eco91I GGTNACC 1 cut(s) 637
EcoICRI GAGCTC 2 cut(s) 280, 715
EcoO65I GGTNACC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 1068
EcoT38I GRGCYC 3 cut(s) 282, 717, 1043
ErhI CCWWGG 1 cut(s) 1068
FaeI CATG 5 cut(s) 879, 1018, 1039, 1066, 1072
FaqI GGGAC 2 cut(s) 780, 921
FatI CATG 5 cut(s) 875, 1014, 1035, 1062, 1068
FauI CCCGC 3 cut(s) 676, 875, 978
Fnu4HI GCNGC 9 cut(s) 69, 196, 296, 438, 490, 672, 726, 729, 804
FokI GGATG 2 cut(s) 959, 960
FriOI GRGCYC 3 cut(s) 282, 717, 1043
Fsp4HI GCNGC 9 cut(s) 69, 196, 296, 438, 490, 672, 726, 729, 804
FspBI CTAG 4 cut(s) 14, 254, 1026, 1087
GlaI GCGC 3 cut(s) 76, 141, 441
GluI GCNGC 9 cut(s) 69, 196, 296, 438, 490, 672, 726, 729, 804
GsuI CTGGAG 2 cut(s) 527, 701
HaeIII GGCC 5 cut(s) 131, 195, 651, 680, 1067
HgaI GACGC 2 cut(s) 473, 800
HhaI GCGC 3 cut(s) 77, 142, 442
Hin1II CATG 5 cut(s) 879, 1018, 1039, 1066, 1072
Hin6I GCGC 3 cut(s) 75, 140, 440
HinP1I GCGC 3 cut(s) 75, 140, 440
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 6 cut(s) 43, 95, 333, 768, 928, 1073
HphI GGTGA 4 cut(s) 52, 274, 649, 710
Hpy166II GTNNAC 3 cut(s) 460, 505, 707
Hpy188I TCNGA 5 cut(s) 92, 250, 434, 583, 982
Hpy188III TCNNGA 4 cut(s) 601, 718, 754, 1015
Hpy8I GTNNAC 3 cut(s) 460, 505, 707
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 2 cut(s) 535, 621
HpyCH4III ACNGT 2 cut(s) 40, 894
HpyCH4IV ACGT 1 cut(s) 863
HpyCH4V TGCA 4 cut(s) 533, 707, 818, 1006
HpyF3I CTNAG 7 cut(s) 63, 558, 602, 777, 833, 981, 1052
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 5 cut(s) 879, 1018, 1039, 1066, 1072
HspAI GCGC 3 cut(s) 75, 140, 440
KpnI GGTACC 1 cut(s) 524
Kzo9I GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
LmnI GCTCC 4 cut(s) 250, 434, 720, 941
Lsp1109I GCAGC 6 cut(s) 80, 282, 449, 737, 740, 790
MaeI CTAG 4 cut(s) 14, 254, 1026, 1087
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 3 cut(s) 637, 836, 859
MalI GATC 6 cut(s) 225, 247, 377, 538, 966, 1013
MbiI CCGCTC 1 cut(s) 985
MboI GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
MboII GAAGA 1 cut(s) 727
MflI RGATCY 2 cut(s) 375, 964
MhlI GDGCHC 7 cut(s) 38, 282, 427, 709, 717, 938, 1043
MlsI TGGCCA 2 cut(s) 131, 1067
MluCI AATT 5 cut(s) 121, 327, 356, 594, 614
MluNI TGGCCA 2 cut(s) 131, 1067
MlyI GAGTC 3 cut(s) 89, 762, 1067
MmeI TCCRAC 2 cut(s) 228, 963
Mox20I TGGCCA 2 cut(s) 131, 1067
MscI TGGCCA 2 cut(s) 131, 1067
MseI TTAA 1 cut(s) 212
MslI CAYNNNNRTG 2 cut(s) 1061, 1067
Msp20I TGGCCA 2 cut(s) 131, 1067
MspR9I CCNGG 4 cut(s) 844, 944, 1021, 1078
MvnI CGCG 3 cut(s) 75, 140, 142
NciI CCSGG 4 cut(s) 844, 944, 1021, 1078
NcoI CCATGG 1 cut(s) 1068
NdeII GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
NlaIII CATG 5 cut(s) 879, 1018, 1039, 1066, 1072
NlaIV GGNNCC 2 cut(s) 522, 966
NmeAIII GCCGAG 1 cut(s) 465
NmuCI GTSAC 1 cut(s) 637
NspI RCATGY 1 cut(s) 879
PagI TCATGA 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 923
PfeI GAWTC 3 cut(s) 43, 333, 928
PflFI GACNNNGTC 2 cut(s) 500, 1021
PfoI TCCNGGA 1 cut(s) 1076
PinAI ACCGGT 2 cut(s) 347, 641
PkrI GCNGC 9 cut(s) 70, 197, 297, 439, 491, 673, 727, 730, 805
PleI GAGTC 3 cut(s) 89, 762, 1067
PpsI GAGTC 3 cut(s) 89, 762, 1067
Psp124BI GAGCTC 2 cut(s) 282, 717
PspEI GGTNACC 1 cut(s) 637
PspN4I GGNNCC 2 cut(s) 522, 966
PspPI GGNCC 3 cut(s) 650, 679, 890
PsuI RGATCY 2 cut(s) 375, 964
PsyI GACNNNGTC 2 cut(s) 500, 1021
RsaI GTAC 9 cut(s) 522, 548, 563, 572, 587, 646, 896, 916, 1034
RsaNI GTAC 9 cut(s) 521, 547, 562, 571, 586, 645, 895, 915, 1033
RseI CAYNNNNRTG 2 cut(s) 1061, 1067
SacI GAGCTC 2 cut(s) 282, 717
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 9 cut(s) 69, 196, 296, 438, 490, 672, 726, 729, 804
Sau3AI GATC 6 cut(s) 223, 245, 375, 536, 964, 1011
Sau96I GGNCC 3 cut(s) 650, 679, 890
ScaI AGTACT 2 cut(s) 548, 563
SchI GAGTC 3 cut(s) 89, 762, 1067
ScrFI CCNGG 4 cut(s) 844, 944, 1021, 1078
SduI GDGCHC 7 cut(s) 38, 282, 427, 709, 717, 938, 1043
SinI GGWCC 1 cut(s) 890
SmiMI CAYNNNNRTG 2 cut(s) 1061, 1067
Sse9I AATT 5 cut(s) 121, 327, 356, 594, 614
SsiI CCGC 8 cut(s) 138, 184, 196, 489, 669, 672, 882, 985
SspMI CTAG 4 cut(s) 14, 254, 1026, 1087
SstI GAGCTC 2 cut(s) 282, 717
StyD4I CCNGG 4 cut(s) 842, 942, 1019, 1076
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 2 cut(s) 40, 894
TaiI ACGT 1 cut(s) 866
TaqI TCGA 1 cut(s) 926
TasI AATT 5 cut(s) 121, 327, 356, 594, 614
TatI WGTACW 3 cut(s) 546, 561, 1032
TauI GCSGC 3 cut(s) 198, 492, 674
TfiI GAWTC 3 cut(s) 43, 333, 928
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 6 cut(s) 68, 295, 437, 725, 728, 803
Tsp45I GTSAC 1 cut(s) 637
TspDTI ATGAA 2 cut(s) 787, 1061
TspRI CASTG 1 cut(s) 280
Tth111I GACNNNGTC 2 cut(s) 500, 1021
VneI GTGCAC 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 890
XapI RAATTY 1 cut(s) 594
XceI RCATGY 1 cut(s) 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 1066
XspI CTAG 4 cut(s) 14, 254, 1026, 1087
ZrmI AGTACT 2 cut(s) 548, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.