MD10G1171100.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012747
Physical Location & Seq
Reverse (-)
26375971 .. 26380350
4380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1171100.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGTCAGGTACAACTCCCAGCTGCAAGGTCTTTGCTGCTGTCGCTCTGATAGTGGCAATGGCGGCAATTGCACCTGAAGCCGAGGCAGCTCGTGCTTTCTTTGTGTTTGGGGACTCACTCGTCGACAGTGGCAACAACAACTACTTAGCGACCACTGCACGTGCCGACTCTCCTCCCTACGGCATTGATTTTCCGACCCACCGCCCCACCGGCCGCTTCTCCAACGGCTTAAACCTCCCCGACATTATCAGTGAGCAAATTGGATCTGAACGCGTGTTGCCCTATTTGAATAGTGAGCTCACAGGAGAAAAGCTGCTTATTGGAGCCAACTTTGCTTCTGCAGGAATTGGAATCCTCAACGATACCGGGGTTCAATTTATTACCATAATAAGGATCTCCATGCAGTTTGAATATTTCCAACAATACCAGCAACGGCTGACTGCACTTGTTGGGGCCGAGCAGGCAACACGGCTGGTGAACAATGCACTTGTGCTCATAACGCTTGGTGGCAATGACTTCGTCAACAACTATTTCATCCAACCTTTCTCTTTACGATCACGGCAATACTCCGTCCCCGATTTTTCCCGTTACCTCATCTCCGAGTACCGAAAAATTCTCCTGAGATTATACGAATTAGGTGCCCGGAAGGTATTGGTAACTGGTACGGGCCCATTGGGTTGTGTTCCATCTGAGCTAGCCCAGAGGAGTCAAAATGGTGAGTGCAACCCAGAACTACAAAATGCAGGAGCAATCTTCAACCCACAACTCGTCCAGATGGTTCAAGGACTCAACAAGCAGCTCGGTTCAGATATTTTTATCACTGCTAATGCATTTGATATGCACATGGACTTCATTTCCGACCCCCAAAAATTTGGTTTTGTGACATCAAAAGTGGCATGTTGTGGACAAGGACCCTATAATGGGTTGGGACAGTGCAATAGATTGTCAAACCTGTGCCCAAACAGGGATCTCTATGCTTTCTGGGATGCTTTCCATCCAACTGAGAAAGCAACCAGGCTAATTGTGCAGCAGATTATGTCCGGGTCTAGCAAGTACATGAACCCAATGAACTTAAGCACCATAATGGCCTTGGACTCCATGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

40.34

Weight (kDa)

5.97

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 344 1.1e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AccB1I GGYRCC 1 cut(s) 638
AccI GTMKAC 1 cut(s) 123
AccII CGCG 1 cut(s) 273
AciI CCGC 3 cut(s) 62, 202, 214
AclWI GGATC 3 cut(s) 271, 401, 975
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 2 cut(s) 612, 869
AcuI CTGAAG 1 cut(s) 96
AcvI CACGTG 1 cut(s) 161
AfaI GTAC 4 cut(s) 10, 605, 664, 1055
AfiI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
AflII CTTAAG 1 cut(s) 1072
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 5 cut(s) 289, 374, 410, 757, 782
AjnI CCWGG 1 cut(s) 1013
AluBI AGCT 6 cut(s) 21, 89, 298, 313, 694, 799
AluI AGCT 6 cut(s) 21, 89, 298, 313, 694, 799
Alw21I GWGCWC 2 cut(s) 300, 495
AlwI GGATC 3 cut(s) 271, 401, 975
AoxI GGCC 4 cut(s) 211, 453, 667, 1086
ApaI GGGCCC 1 cut(s) 671
ApeKI GCWGC 6 cut(s) 21, 35, 86, 313, 796, 1027
ApoI RAATTY 2 cut(s) 612, 869
AspS9I GGNCC 4 cut(s) 453, 667, 668, 911
AsuC2I CCSGG 3 cut(s) 367, 643, 1042
AsuHPI GGTGA 2 cut(s) 487, 728
AsuNHI GCTAGC 1 cut(s) 694
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 3 cut(s) 643, 671, 959
BaeI ACNNNNGTAYC 2 cut(s) 354, 387
BanI GGYRCC 1 cut(s) 638
BanII GRGCYC 2 cut(s) 300, 671
BauI CACGAG 1 cut(s) 90
BbrPI CACGTG 1 cut(s) 161
Bbv12I GWGCWC 2 cut(s) 300, 495
BbvI GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BccI CCATC 3 cut(s) 694, 769, 1002
BceAI ACGGC 5 cut(s) 196, 241, 449, 485, 575
BcgI CGANNNNNNTGC 4 cut(s) 499, 533, 620, 654
BciT130I CCWGG 1 cut(s) 1015
BcnI CCSGG 3 cut(s) 367, 643, 1042
BfaI CTAG 2 cut(s) 695, 1047
BfmI CTRYAG 1 cut(s) 339
BfrI CTTAAG 1 cut(s) 1072
BglI GCCNNNNNGGC 2 cut(s) 210, 461
BisI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
BlsI GCNGC 8 cut(s) 23, 37, 64, 88, 215, 315, 798, 1029
Bme1390I CCNGG 4 cut(s) 367, 643, 1015, 1042
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 4 cut(s) 453, 667, 668, 911
BmiI GGNNCC 5 cut(s) 325, 454, 640, 669, 913
BmrFI CCNGG 4 cut(s) 367, 643, 1015, 1042
BmsI GCATC 1 cut(s) 976
BmtI GCTAGC 1 cut(s) 698
BpuMI CCSGG 3 cut(s) 367, 643, 1042
BsaAI YACGTR 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 81, 366, 1089
BsaXI ACNNNNNCTCC 2 cut(s) 581, 611
Bsc4I CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 664
Bse3DI GCAATG 2 cut(s) 63, 517
BseBI CCWGG 1 cut(s) 1015
BseDI CCNNGG 3 cut(s) 81, 366, 1089
BseGI GGATG 3 cut(s) 534, 991, 994
BseLI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
BseMI GCAATG 2 cut(s) 63, 517
BseMII CTCAG 3 cut(s) 611, 681, 993
BseNI ACTGG 1 cut(s) 664
BseRI GAGGAG 2 cut(s) 162, 718
BseSI GKGCMC 3 cut(s) 643, 671, 959
BseX3I CGGCCG 1 cut(s) 211
BseXI GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BseYI CCCAGC 1 cut(s) 17
BsgI GTGCAG 3 cut(s) 141, 426, 1046
Bsh1236I CGCG 1 cut(s) 273
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 4 cut(s) 213, 455, 669, 1088
BshNI GGYRCC 1 cut(s) 638
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 2 cut(s) 300, 495
BsiSI CCGG 4 cut(s) 210, 366, 643, 1041
BslFI GGGAC 3 cut(s) 125, 557, 942
BslI CCNNNNNNNGG 5 cut(s) 179, 390, 920, 921, 964
BsmFI GGGAC 3 cut(s) 125, 557, 942
BsnI GGCC 4 cut(s) 213, 455, 669, 1088
Bsp120I GGGCCC 1 cut(s) 667
Bsp1286I GDGCHC 5 cut(s) 300, 495, 643, 671, 959
Bsp143I GATC 4 cut(s) 263, 393, 554, 967
BspACI CCGC 3 cut(s) 62, 202, 214
BspANI GGCC 4 cut(s) 213, 455, 669, 1088
BspCNI CTCAG 3 cut(s) 612, 682, 994
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 5 cut(s) 325, 454, 640, 669, 913
BspMAI CTGCAG 1 cut(s) 343
BspOI GCTAGC 1 cut(s) 698
BspPI GGATC 3 cut(s) 271, 401, 975
BspT107I GGYRCC 1 cut(s) 638
BspTI CTTAAG 1 cut(s) 1072
BsrDI GCAATG 2 cut(s) 63, 517
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 81, 366, 1089
BssMI GATC 4 cut(s) 263, 393, 554, 967
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 1015
Bst4CI ACNGT 2 cut(s) 128, 933
BstAFI CTTAAG 1 cut(s) 1072
BstAPI GCANNNNNTGC 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 462, 696
BstDEI CTNAG 4 cut(s) 145, 620, 690, 1002
BstF5I GGATG 3 cut(s) 534, 991, 994
BstFNI CGCG 1 cut(s) 273
BstKTI GATC 4 cut(s) 266, 396, 557, 970
BstMBI GATC 4 cut(s) 263, 393, 554, 967
BstMCI CGRYCG 1 cut(s) 214
BstNI CCWGG 1 cut(s) 1015
BstNSI RCATGY 1 cut(s) 900
BstSCI CCNGG 4 cut(s) 365, 641, 1013, 1040
BstSFI CTRYAG 1 cut(s) 339
BstSLI GKGCMC 3 cut(s) 643, 671, 959
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
BstX2I RGATCY 3 cut(s) 263, 393, 967
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 3 cut(s) 263, 393, 967
BstZI CGGCCG 1 cut(s) 211
BsuRI GGCC 4 cut(s) 213, 455, 669, 1088
BtsCI GGATG 3 cut(s) 534, 991, 994
BtsI GCAGTG 2 cut(s) 153, 819
BtsIMutI CAGTG 5 cut(s) 133, 153, 256, 819, 938
Cac8I GCNNGC 2 cut(s) 462, 696
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 4 cut(s) 453, 667, 668, 911
Csp6I GTAC 4 cut(s) 9, 604, 663, 1054
CviAII CATG 5 cut(s) 400, 844, 897, 1057, 1099
CviQI GTAC 4 cut(s) 9, 604, 663, 1054
DdeI CTNAG 4 cut(s) 145, 620, 690, 1002
DpnI GATC 4 cut(s) 265, 395, 556, 969
DpnII GATC 4 cut(s) 263, 393, 554, 967
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 211
EagI CGGCCG 1 cut(s) 211
Ecl136II GAGCTC 1 cut(s) 298
EclXI CGGCCG 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 1089
Eco24I GRGCYC 2 cut(s) 300, 671
Eco47I GGWCC 1 cut(s) 911
Eco52I CGGCCG 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 96
Eco72I CACGTG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 911
EcoRII CCWGG 1 cut(s) 1013
EcoT14I CCWWGG 1 cut(s) 1089
EcoT22I ATGCAT 1 cut(s) 832
EcoT38I GRGCYC 2 cut(s) 300, 671
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 5 cut(s) 403, 847, 900, 1060, 1102
FaqI GGGAC 3 cut(s) 125, 557, 942
FatI CATG 5 cut(s) 399, 843, 896, 1056, 1098
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
FokI GGATG 3 cut(s) 521, 981, 998
FriOI GRGCYC 2 cut(s) 300, 671
Fsp4HI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
FspBI CTAG 2 cut(s) 695, 1047
GluI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
GsaI CCCAGC 1 cut(s) 21
HaeIII GGCC 4 cut(s) 213, 455, 669, 1088
HapII CCGG 4 cut(s) 210, 366, 643, 1041
Hin1II CATG 5 cut(s) 403, 847, 900, 1060, 1102
HincII GTYRAC 2 cut(s) 124, 523
HindII GTYRAC 2 cut(s) 124, 523
HinfI GANTC 6 cut(s) 113, 167, 351, 706, 786, 1094
HpaII CCGG 4 cut(s) 210, 366, 643, 1041
HphI GGTGA 2 cut(s) 487, 728
Hpy166II GTNNAC 4 cut(s) 124, 478, 523, 905
Hpy188I TCNGA 7 cut(s) 48, 195, 268, 601, 691, 808, 859
Hpy188III TCNNGA 2 cut(s) 619, 772
Hpy8I GTNNAC 4 cut(s) 124, 478, 523, 905
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 2 cut(s) 128, 933
HpyCH4IV ACGT 1 cut(s) 160
HpyF3I CTNAG 4 cut(s) 145, 620, 690, 1002
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 5 cut(s) 403, 847, 900, 1060, 1102
Kzo9I GATC 4 cut(s) 263, 393, 554, 967
LmnI GCTCC 2 cut(s) 323, 746
Lsp1109I GCAGC 6 cut(s) 8, 22, 98, 300, 808, 1039
LweI GCATC 1 cut(s) 976
MaeI CTAG 2 cut(s) 695, 1047
MaeII ACGT 1 cut(s) 160
MaeIII GTNAC 3 cut(s) 587, 655, 880
MalI GATC 4 cut(s) 265, 395, 556, 969
MboI GATC 4 cut(s) 263, 393, 554, 967
MboII GAAGA 1 cut(s) 745
MfeI CAATTG 1 cut(s) 66
MflI RGATCY 3 cut(s) 263, 393, 967
MhlI GDGCHC 5 cut(s) 300, 495, 643, 671, 959
MluCI AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
MluI ACGCGT 1 cut(s) 271
MlyI GAGTC 5 cut(s) 107, 161, 715, 780, 1088
MmeI TCCRAC 6 cut(s) 218, 246, 442, 562, 882, 1022
MnlI CCTC 6 cut(s) 76, 183, 245, 365, 602, 696
Mph1103I ATGCAT 1 cut(s) 832
MseI TTAA 2 cut(s) 230, 1073
MslI CAYNNNNRTG 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 21
MspCI CTTAAG 1 cut(s) 1072
MspI CCGG 4 cut(s) 210, 366, 643, 1041
MspR9I CCNGG 4 cut(s) 367, 643, 1015, 1042
MunI CAATTG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 1015
MvnI CGCG 1 cut(s) 273
NciI CCSGG 3 cut(s) 367, 643, 1042
NdeII GATC 4 cut(s) 263, 393, 554, 967
NheI GCTAGC 1 cut(s) 694
NlaIII CATG 5 cut(s) 403, 847, 900, 1060, 1102
NlaIV GGNNCC 5 cut(s) 325, 454, 640, 669, 913
NmeAIII GCCGAG 2 cut(s) 106, 481
NmuCI GTSAC 1 cut(s) 880
NsiI ATGCAT 1 cut(s) 832
NspI RCATGY 1 cut(s) 900
PfeI GAWTC 1 cut(s) 351
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 8 cut(s) 23, 37, 64, 88, 215, 315, 798, 1029
PleI GAGTC 5 cut(s) 107, 161, 714, 780, 1088
PmaCI CACGTG 1 cut(s) 161
PmlI CACGTG 1 cut(s) 161
PpsI GAGTC 5 cut(s) 107, 161, 714, 780, 1088
Ppu21I YACGTR 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 300
Psp5II RGGWCCY 1 cut(s) 911
Psp6I CCWGG 1 cut(s) 1013
PspCI CACGTG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 17
PspGI CCWGG 1 cut(s) 1013
PspN4I GGNNCC 5 cut(s) 325, 454, 640, 669, 913
PspOMI GGGCCC 1 cut(s) 667
PspPI GGNCC 4 cut(s) 453, 667, 668, 911
PspPPI RGGWCCY 1 cut(s) 911
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 3 cut(s) 263, 393, 967
PsyI GACNNNGTC 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 21
RsaI GTAC 4 cut(s) 10, 605, 664, 1055
RsaNI GTAC 4 cut(s) 9, 604, 663, 1054
RseI CAYNNNNRTG 1 cut(s) 1082
SacI GAGCTC 1 cut(s) 300
SalI GTCGAC 1 cut(s) 122
SaqAI TTAA 2 cut(s) 230, 1073
SatI GCNGC 8 cut(s) 22, 36, 63, 87, 214, 314, 797, 1028
Sau3AI GATC 4 cut(s) 263, 393, 554, 967
Sau96I GGNCC 4 cut(s) 453, 667, 668, 911
SchI GAGTC 5 cut(s) 107, 161, 715, 780, 1088
ScrFI CCNGG 4 cut(s) 367, 643, 1015, 1042
SduI GDGCHC 5 cut(s) 300, 495, 643, 671, 959
SfaNI GCATC 1 cut(s) 976
SfcI CTRYAG 1 cut(s) 339
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 1 cut(s) 1082
SmlI CTYRAG 1 cut(s) 1072
SmoI CTYRAG 1 cut(s) 1072
Sse9I AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
SsiI CCGC 3 cut(s) 62, 202, 214
SspI AATATT 1 cut(s) 413
SspMI CTAG 2 cut(s) 695, 1047
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 4 cut(s) 365, 641, 1013, 1040
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 2 cut(s) 128, 933
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 123
TasI AATT 8 cut(s) 66, 258, 345, 374, 612, 632, 869, 1020
TatI WGTACW 1 cut(s) 1053
TauI GCSGC 2 cut(s) 65, 216
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 2 cut(s) 230, 1073
Tru9I TTAA 2 cut(s) 230, 1073
TscAI CASTG 5 cut(s) 133, 160, 256, 826, 938
TseFI GTSAC 1 cut(s) 880
TseI GCWGC 6 cut(s) 21, 35, 86, 313, 796, 1027
Tsp45I GTSAC 1 cut(s) 880
TspDTI ATGAA 4 cut(s) 523, 841, 1073, 1082
TspGWI ACGGA 1 cut(s) 559
TspRI CASTG 5 cut(s) 133, 160, 256, 826, 938
Tth111I GACNNNGTC 1 cut(s) 518
Vha464I CTTAAG 1 cut(s) 1072
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 2 cut(s) 612, 869
XceI RCATGY 1 cut(s) 900
XcmI CCANNNNNNNNNTGG 1 cut(s) 1087
XmiI GTMKAC 1 cut(s) 123
XspI CTAG 2 cut(s) 695, 1047
Zsp2I ATGCAT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.