FvH4_2g19880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012747
Physical Location & Seq
Reverse (-)
16796137 .. 16798310
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g19880.t1

Sequence Viewer

Length: 1110 bp
ATGTCAACACCTCCTAGGGTTTGTTCCAGTGTCACCATGGCTGTTGCCCTAGTATTAATGGTGATACTGCCTGGACCTCCTCAAGCCGAGGCAGCTCGCGCTTTCTTTGTGTTTGGAGACTCACTTGTGGACAATGGCAACAACAACTACTTGGCCACCACTGCACGTGCCGATTCTCCTCCCTACGGAATTGATTTCCCGACCCACCGCCCCACCGGCCACTTCTCCAACGGTTTGAACCTCCCTGACCTGGTCAGTGAACAAATGGGATCAGAGCTACTCTTGCCATACTTAAGCAGTGAGCTTACCGGACAAAAGCTGCTTACTGGTGCCAACTTTGCTTCTGCTGGAATTGGAATCCTCAATGATACTGGGGTTCAATTTGTCAACATAATAAGGATCTTCAAGCAGTTTGAGCTGTTCCAGCAATACCAACAACGAGTGAGTGCCCTAATCGGAGGAGCTCAAGCCAAGAGGCTAGTAAACGATGCTCTTGTCCTTGTAACTCTTGGCGGCAATGACTTCGTTAACAACTATTTTTTGGTACCCTTCTCTGCAAGATCCCGCCAGTACTCTATTTCTCAGTACTCCCAGTACCTCATCTCCGAGTACCGCAAAATTCTTCTGAGGCTATATGAATTAGGAGGGAGAAGGGTGTTGGTGACTGGTACAGGTCCATTGGGCTGTGTTCCGGCCGCGATGGCGCAGAGGAGTAGGAATGGTGAATGTGCACCAGAGCTGCAAAGGGCAGCAGCCATCTTCAATCCCTTGCTTGTACAGATGGTTCAGGGACTCAACGCACAGCTTGGTACTGAAGCTTTTGTTGCTGCAAATGCTCATGCAAGTATGGACTTCCTCAGCAACCCCCGGAAATATGGCTTTGTTACATCAAGGGTGGTATGTTGCGGGCAAGGACCGTACAATGGGGTGGGACTGTGTACCCCGTTGTCGAATCTGTGCTCTAACCGTGATGTCTATGCATTTTGGGATCCTTTCCATCCATCCGAAAGGGCGAACCGGCTGATTGTGCAGACAATGATGACCGGATCTAGCAAGTACATGAACCCTATGAACCTGAGCACCATCATGGCCATGGACTCCAGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

40.22

Weight (kDa)

8.82

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 343 2.2e-29 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 544
AccB1I GGYRCC 2 cut(s) 329, 544
AccII CGCG 2 cut(s) 99, 698
AciI CCGC 6 cut(s) 208, 513, 565, 613, 696, 906
AclWI GGATC 6 cut(s) 277, 407, 555, 983, 996, 1054
AcoI YGGCCR 4 cut(s) 153, 217, 693, 1089
AcsI RAATTY 1 cut(s) 618
AcuI CTGAAG 1 cut(s) 834
AcvI CACGTG 1 cut(s) 167
AfiI CCNNNNNNNGG 3 cut(s) 185, 250, 923
AflII CTTAAG 1 cut(s) 292
AgsI TTSAA 4 cut(s) 238, 380, 406, 763
AjnI CCWGG 3 cut(s) 70, 249, 1100
AluBI AGCT 9 cut(s) 95, 277, 304, 319, 418, 464, 739, 805, 818
AluI AGCT 9 cut(s) 95, 277, 304, 319, 418, 464, 739, 805, 818
Alw21I GWGCWC 4 cut(s) 466, 733, 962, 1082
Alw26I GTCTC 1 cut(s) 111
Alw44I GTGCAC 1 cut(s) 729
AlwI GGATC 6 cut(s) 277, 407, 555, 983, 996, 1054
AoxI GGCC 4 cut(s) 153, 217, 693, 1089
ApaLI GTGCAC 1 cut(s) 729
ApeKI GCWGC 6 cut(s) 92, 319, 739, 749, 752, 827
ApoI RAATTY 1 cut(s) 618
AseI ATTAAT 1 cut(s) 56
Asp718I GGTACC 1 cut(s) 544
AspA2I CCTAGG 1 cut(s) 14
AspLEI GCGC 2 cut(s) 101, 706
AspS9I GGNCC 3 cut(s) 74, 674, 914
AsuC2I CCSGG 1 cut(s) 868
AsuHPI GGTGA 4 cut(s) 25, 73, 673, 734
AvaII GGWCC 3 cut(s) 74, 674, 914
AvrII CCTAGG 1 cut(s) 14
BaeGI GKGCMC 2 cut(s) 451, 733
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BalI TGGCCA 2 cut(s) 155, 1091
BamHI GGATCC 1 cut(s) 988
BanI GGYRCC 2 cut(s) 329, 544
BanII GRGCYC 1 cut(s) 466
BbrPI CACGTG 1 cut(s) 167
Bbv12I GWGCWC 4 cut(s) 466, 733, 962, 1082
BbvCI CCTCAGC 1 cut(s) 857
BbvI GCAGC 6 cut(s) 104, 306, 726, 761, 764, 814
BccI CCATC 6 cut(s) 694, 764, 775, 1005, 1009, 1091
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 3 cut(s) 72, 251, 1102
BcnI CCSGG 1 cut(s) 868
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 5 cut(s) 15, 50, 479, 1050, 1108
BfrI CTTAAG 1 cut(s) 292
BglI GCCNNNNNGGC 2 cut(s) 216, 701
BisI GCNGC 8 cut(s) 93, 320, 514, 696, 740, 750, 753, 828
BlnI CCTAGG 1 cut(s) 14
BlsI GCNGC 8 cut(s) 94, 321, 515, 697, 741, 751, 754, 829
BmcAI AGTACT 2 cut(s) 572, 587
Bme1390I CCNGG 4 cut(s) 72, 251, 868, 1102
Bme18I GGWCC 3 cut(s) 74, 674, 914
BmgT120I GGNCC 3 cut(s) 74, 674, 914
BmiI GGNNCC 3 cut(s) 331, 546, 990
BmrFI CCNGG 4 cut(s) 72, 251, 868, 1102
BmrI ACTGGG 2 cut(s) 381, 586
BmsI GCATC 1 cut(s) 478
BmuI ACTGGG 2 cut(s) 381, 586
BpmI CTGGAG 1 cut(s) 1084
Bpu10I CCTNAGC 2 cut(s) 857, 1076
BpuEI CTTGAG 2 cut(s) 66, 450
BpuMI CCSGG 1 cut(s) 868
BsaAI YACGTR 1 cut(s) 167
BsaJI CCNNGG 5 cut(s) 14, 36, 87, 866, 1092
BsaWI WCCGGW 2 cut(s) 308, 1043
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 250, 923
Bse118I RCCGGY 2 cut(s) 215, 1017
Bse1I ACTGG 6 cut(s) 27, 331, 376, 568, 592, 670
Bse3DI GCAATG 1 cut(s) 523
BseBI CCWGG 3 cut(s) 72, 251, 1102
BseDI CCNNGG 5 cut(s) 14, 36, 87, 866, 1092
BseGI GGATG 2 cut(s) 997, 1001
BseLI CCNNNNNNNGG 3 cut(s) 185, 250, 923
BseMI GCAATG 1 cut(s) 523
BseMII CTCAG 4 cut(s) 596, 617, 871, 1067
BseNI ACTGG 6 cut(s) 27, 331, 376, 568, 592, 670
BseRI GAGGAG 4 cut(s) 69, 168, 474, 724
BseSI GKGCMC 2 cut(s) 451, 733
BseX3I CGGCCG 1 cut(s) 693
BseXI GCAGC 6 cut(s) 104, 306, 726, 761, 764, 814
BsgI GTGCAG 2 cut(s) 147, 1049
Bsh1236I CGCG 2 cut(s) 99, 698
Bsh1285I CGRYCG 1 cut(s) 696
BshFI GGCC 4 cut(s) 155, 219, 695, 1091
BshNI GGYRCC 2 cut(s) 329, 544
BsiEI CGRYCG 1 cut(s) 696
BsiHKAI GWGCWC 4 cut(s) 466, 733, 962, 1082
BsiSI CCGG 6 cut(s) 216, 309, 692, 868, 1018, 1044
BslFI GGGAC 2 cut(s) 804, 945
BslI CCNNNNNNNGG 3 cut(s) 185, 250, 923
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 2 cut(s) 804, 945
BsnI GGCC 4 cut(s) 155, 219, 695, 1091
Bsp1286I GDGCHC 5 cut(s) 451, 466, 733, 962, 1082
Bsp1407I TGTACA 1 cut(s) 775
Bsp143I GATC 5 cut(s) 269, 399, 560, 988, 1046
Bsp19I CCATGG 2 cut(s) 36, 1092
BspACI CCGC 6 cut(s) 208, 513, 565, 613, 696, 906
BspANI GGCC 4 cut(s) 155, 219, 695, 1091
BspCNI CTCAG 4 cut(s) 595, 618, 870, 1068
BspFNI CGCG 2 cut(s) 99, 698
BspLI GGNNCC 3 cut(s) 331, 546, 990
BspPI GGATC 6 cut(s) 277, 407, 555, 983, 996, 1054
BspT107I GGYRCC 2 cut(s) 329, 544
BspTI CTTAAG 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 523
BsrFI RCCGGY 2 cut(s) 215, 1017
BsrGI TGTACA 1 cut(s) 775
BsrI ACTGG 6 cut(s) 27, 331, 376, 568, 592, 670
BssAI RCCGGY 2 cut(s) 215, 1017
BssECI CCNNGG 5 cut(s) 14, 36, 87, 866, 1092
BssMI GATC 5 cut(s) 269, 399, 560, 988, 1046
BssT1I CCWWGG 3 cut(s) 14, 36, 1092
Bst2UI CCWGG 3 cut(s) 72, 251, 1102
Bst4CI ACNGT 4 cut(s) 233, 918, 936, 968
BstAFI CTTAAG 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 775
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 97, 908
BstDEI CTNAG 4 cut(s) 582, 626, 857, 1076
BstDSI CCRYGG 2 cut(s) 36, 1092
BstF5I GGATG 2 cut(s) 997, 1001
BstFNI CGCG 2 cut(s) 99, 698
BstHHI GCGC 2 cut(s) 101, 706
BstKTI GATC 5 cut(s) 272, 402, 563, 991, 1049
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 5 cut(s) 269, 399, 560, 988, 1046
BstMCI CGRYCG 1 cut(s) 696
BstNI CCWGG 3 cut(s) 72, 251, 1102
BstSCI CCNGG 4 cut(s) 70, 249, 866, 1100
BstSLI GKGCMC 2 cut(s) 451, 733
BstUI CGCG 2 cut(s) 99, 698
BstV1I GCAGC 6 cut(s) 104, 306, 726, 761, 764, 814
BstX2I RGATCY 4 cut(s) 399, 560, 988, 1046
BstYI RGATCY 4 cut(s) 399, 560, 988, 1046
BstZI CGGCCG 1 cut(s) 693
BsuRI GGCC 4 cut(s) 155, 219, 695, 1091
BtgI CCRYGG 2 cut(s) 36, 1092
BtgZI GCGATG 1 cut(s) 713
BtsCI GGATG 2 cut(s) 997, 1001
BtsI GCAGTG 2 cut(s) 159, 304
BtsIMutI CAGTG 4 cut(s) 34, 159, 262, 304
Cac8I GCNNGC 2 cut(s) 97, 908
CfoI GCGC 2 cut(s) 101, 706
Cfr10I RCCGGY 2 cut(s) 215, 1017
Cfr13I GGNCC 3 cut(s) 74, 674, 914
CsiI ACCWGGT 1 cut(s) 249
CviAII CATG 5 cut(s) 37, 839, 1060, 1087, 1093
DdeI CTNAG 4 cut(s) 582, 626, 857, 1076
DpnI GATC 5 cut(s) 271, 401, 562, 990, 1048
DpnII GATC 5 cut(s) 269, 399, 560, 988, 1046
EaeI YGGCCR 4 cut(s) 153, 217, 693, 1089
EagI CGGCCG 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 464
EclXI CGGCCG 1 cut(s) 693
Eco130I CCWWGG 3 cut(s) 14, 36, 1092
Eco24I GRGCYC 1 cut(s) 466
Eco47I GGWCC 3 cut(s) 74, 674, 914
Eco52I CGGCCG 1 cut(s) 693
Eco53kI GAGCTC 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 834
Eco72I CACGTG 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 464
EcoRII CCWGG 3 cut(s) 70, 249, 1100
EcoT14I CCWWGG 3 cut(s) 14, 36, 1092
EcoT22I ATGCAT 1 cut(s) 982
EcoT38I GRGCYC 1 cut(s) 466
ErhI CCWWGG 3 cut(s) 14, 36, 1092
FaeI CATG 5 cut(s) 40, 842, 1063, 1090, 1096
FaqI GGGAC 2 cut(s) 804, 945
FatI CATG 5 cut(s) 36, 838, 1059, 1086, 1092
FauI CCCGC 2 cut(s) 572, 899
Fnu4HI GCNGC 8 cut(s) 93, 320, 514, 696, 740, 750, 753, 828
FokI GGATG 2 cut(s) 984, 988
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 8 cut(s) 93, 320, 514, 696, 740, 750, 753, 828
FspBI CTAG 5 cut(s) 15, 50, 479, 1050, 1108
GlaI GCGC 2 cut(s) 100, 705
GluI GCNGC 8 cut(s) 93, 320, 514, 696, 740, 750, 753, 828
GsuI CTGGAG 1 cut(s) 1084
HaeIII GGCC 4 cut(s) 155, 219, 695, 1091
HapII CCGG 6 cut(s) 216, 309, 692, 868, 1018, 1044
HhaI GCGC 2 cut(s) 101, 706
Hin1II CATG 5 cut(s) 40, 842, 1063, 1090, 1096
Hin6I GCGC 2 cut(s) 99, 704
HinP1I GCGC 2 cut(s) 99, 704
HincII GTYRAC 3 cut(s) 6, 388, 529
HindII GTYRAC 3 cut(s) 6, 388, 529
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 6 cut(s) 119, 173, 357, 792, 952, 1097
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 6 cut(s) 216, 309, 692, 868, 1018, 1044
HphI GGTGA 4 cut(s) 25, 73, 673, 734
Hpy166II GTNNAC 8 cut(s) 6, 130, 260, 388, 484, 529, 731, 939
Hpy188I TCNGA 5 cut(s) 274, 458, 607, 627, 1006
Hpy188III TCNNGA 1 cut(s) 199
Hpy8I GTNNAC 8 cut(s) 6, 130, 260, 388, 484, 529, 731, 939
HpyAV CCTTC 2 cut(s) 559, 645
HpyCH4III ACNGT 4 cut(s) 233, 918, 936, 968
HpyCH4IV ACGT 1 cut(s) 166
HpyCH4V TGCA 8 cut(s) 164, 557, 731, 742, 830, 842, 980, 1030
HpyF3I CTNAG 4 cut(s) 582, 626, 857, 1076
HpySE526I ACGT 1 cut(s) 166
Hsp92II CATG 5 cut(s) 40, 842, 1063, 1090, 1096
HspAI GCGC 2 cut(s) 99, 704
KpnI GGTACC 1 cut(s) 548
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 5 cut(s) 269, 399, 560, 988, 1046
LmnI GCTCC 1 cut(s) 461
Lsp1109I GCAGC 6 cut(s) 104, 306, 726, 761, 764, 814
LweI GCATC 1 cut(s) 478
MabI ACCWGGT 1 cut(s) 249
MaeI CTAG 5 cut(s) 15, 50, 479, 1050, 1108
MaeII ACGT 1 cut(s) 166
MaeIII GTNAC 4 cut(s) 31, 502, 661, 883
MalI GATC 5 cut(s) 271, 401, 562, 990, 1048
MboI GATC 5 cut(s) 269, 399, 560, 988, 1046
MboII GAAGA 3 cut(s) 394, 614, 751
MflI RGATCY 4 cut(s) 399, 560, 988, 1046
MhlI GDGCHC 5 cut(s) 451, 466, 733, 962, 1082
MlsI TGGCCA 2 cut(s) 155, 1091
MluCI AATT 5 cut(s) 189, 351, 380, 618, 638
MluNI TGGCCA 2 cut(s) 155, 1091
MlyI GAGTC 3 cut(s) 113, 786, 1091
MmeI TCCRAC 1 cut(s) 252
Mox20I TGGCCA 2 cut(s) 155, 1091
Mph1103I ATGCAT 1 cut(s) 982
MscI TGGCCA 2 cut(s) 155, 1091
MseI TTAA 3 cut(s) 56, 293, 528
MslI CAYNNNNRTG 2 cut(s) 1085, 1091
Msp20I TGGCCA 2 cut(s) 155, 1091
MspCI CTTAAG 1 cut(s) 292
MspI CCGG 6 cut(s) 216, 309, 692, 868, 1018, 1044
MspR9I CCNGG 4 cut(s) 72, 251, 868, 1102
MvaI CCWGG 3 cut(s) 72, 251, 1102
MvnI CGCG 2 cut(s) 99, 698
NciI CCSGG 1 cut(s) 868
NcoI CCATGG 2 cut(s) 36, 1092
NdeII GATC 5 cut(s) 269, 399, 560, 988, 1046
NlaIII CATG 5 cut(s) 40, 842, 1063, 1090, 1096
NlaIV GGNNCC 3 cut(s) 331, 546, 990
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 31, 661
NsiI ATGCAT 1 cut(s) 982
PfeI GAWTC 3 cut(s) 173, 357, 952
PflFI GACNNNGTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 1100
PkrI GCNGC 8 cut(s) 94, 321, 515, 697, 741, 751, 754, 829
PleI GAGTC 3 cut(s) 113, 786, 1091
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 3 cut(s) 113, 786, 1091
Ppu21I YACGTR 1 cut(s) 167
PshBI ATTAAT 1 cut(s) 56
Psp124BI GAGCTC 1 cut(s) 466
Psp6I CCWGG 3 cut(s) 70, 249, 1100
PspCI CACGTG 1 cut(s) 167
PspGI CCWGG 3 cut(s) 70, 249, 1100
PspN4I GGNNCC 3 cut(s) 331, 546, 990
PspPI GGNCC 3 cut(s) 74, 674, 914
PsrI GAACNNNNNNTAC 2 cut(s) 768, 800
PsuI RGATCY 4 cut(s) 399, 560, 988, 1046
PsyI GACNNNGTC 1 cut(s) 251
RseI CAYNNNNRTG 2 cut(s) 1085, 1091
SacI GAGCTC 1 cut(s) 466
SaqAI TTAA 3 cut(s) 56, 293, 528
SatI GCNGC 8 cut(s) 93, 320, 514, 696, 740, 750, 753, 828
Sau3AI GATC 5 cut(s) 269, 399, 560, 988, 1046
Sau96I GGNCC 3 cut(s) 74, 674, 914
ScaI AGTACT 2 cut(s) 572, 587
SchI GAGTC 3 cut(s) 113, 786, 1091
ScrFI CCNGG 4 cut(s) 72, 251, 868, 1102
SduI GDGCHC 5 cut(s) 451, 466, 733, 962, 1082
SexAI ACCWGGT 1 cut(s) 249
SfaNI GCATC 1 cut(s) 478
SinI GGWCC 3 cut(s) 74, 674, 914
SmiMI CAYNNNNRTG 2 cut(s) 1085, 1091
SmlI CTYRAG 3 cut(s) 81, 292, 465
SmoI CTYRAG 3 cut(s) 81, 292, 465
Sse9I AATT 5 cut(s) 189, 351, 380, 618, 638
SsiI CCGC 6 cut(s) 208, 513, 565, 613, 696, 906
SspMI CTAG 5 cut(s) 15, 50, 479, 1050, 1108
SstI GAGCTC 1 cut(s) 466
StyD4I CCNGG 4 cut(s) 70, 249, 866, 1100
StyI CCWWGG 3 cut(s) 14, 36, 1092
TaaI ACNGT 4 cut(s) 233, 918, 936, 968
TaiI ACGT 1 cut(s) 169
TaqI TCGA 1 cut(s) 950
TasI AATT 5 cut(s) 189, 351, 380, 618, 638
TatI WGTACW 4 cut(s) 570, 585, 775, 1056
TauI GCSGC 2 cut(s) 516, 698
TfiI GAWTC 3 cut(s) 173, 357, 952
Tru1I TTAA 3 cut(s) 56, 293, 528
Tru9I TTAA 3 cut(s) 56, 293, 528
TscAI CASTG 4 cut(s) 34, 166, 262, 304
TseFI GTSAC 2 cut(s) 31, 661
TseI GCWGC 6 cut(s) 92, 319, 739, 749, 752, 827
Tsp45I GTSAC 2 cut(s) 31, 661
TspDTI ATGAA 3 cut(s) 651, 1076, 1085
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 4 cut(s) 34, 166, 262, 304
Tth111I GACNNNGTC 1 cut(s) 251
Vha464I CTTAAG 1 cut(s) 292
VneI GTGCAC 1 cut(s) 729
VpaK11BI GGWCC 3 cut(s) 74, 674, 914
VspI ATTAAT 1 cut(s) 56
XapI RAATTY 1 cut(s) 618
XcmI CCANNNNNNNNNTGG 2 cut(s) 34, 1090
XmaJI CCTAGG 1 cut(s) 14
XspI CTAG 5 cut(s) 15, 50, 479, 1050, 1108
ZrmI AGTACT 2 cut(s) 572, 587
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.