RchiOBHm_Chr6g0285571

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012747
Physical Location & Seq
Reverse (-)
48923736 .. 48925566
1831 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25615

Sequence Viewer

Length: 1221 bp
ATGCCCCTCACATTTCTAAATAAAACCCTAAAAGGCTCTAGTATTCCCAACAACTTAATTTATACTGTAGTCTCCAAAGAAACTCCCGCATTAGCTTTCATGTCTCCTCCTAGTGTTTGTTCCGGTGCCACCATGGCTGTTGCCCTAGTGCTAATGGTGGCAATGAGCACGGTGATTCTCGCACCTCATCAAGCTGAGGCAGCTCGAGCTTTCTTTGTTTTCGGAGACTCCCTTGTGGATAACGGCAACAACAATTACTTGGCCACCACCGCGCGTGCCGATTTTCCTCCCTACGGCATTGATTTCCCTACCCACCGCCCCACCGGCCGCTTCTCCAACGGCTTAAACCTCCCTGATCTCATCAGCGAGCAAATAGGATCGGAGCTAGTCTTGCCATATTTGAGCAGTGAGCTCACCGGACAAAAGCTGCTTATTGGTGCTAACTTTGCTTCTGCTGGAATTGGAATCCTCAATGATACTGGTGTTCAATTTATCAACATAATAAGGATCTTTAGGCAGTTTGAGATGTTCCAGCAATACCAGCAACGAGTGAGTGCCCTAATCGGAGCAGCGCAAGCCAAGAGGCTGGTAAACGACGCTCTTGTCCTCATAACTCTCGGCGGCAATGACTTCGTCAACAACTACTTCTTGGTACCCTTCTCTGCAAGATCACGCCAGTACTCAATCCCTCAGTACTCCCAGTACCTCATCTCCGAGTACCGGAAAATTCTCCGGAGACTATTTGGATTAGGAGGGAGAAGGGTGCTGGTGACCGGTACAGGCCCATTAGGCTGTGTTCCCGCCGCTATGGCCCAGAGGAGTAGGAATGGTGAATGTGCACCAGAGCTCCAGAGGGCAGCAGCCATTTTCAATCCCCTGCTTGTCCAGATGGTTCAGGGGCTCAACGCTCAGCTTGGTACTGACGCTTTCATTGCTGCTAATGCGTTTGCAAATGTGGAGTTCCTCAGTAACCCCCGGAAATATGGCTTTGCTACGTCAAAGGTGGCATGTTGCGGGCAAGGACCGTACAATGGGTTGGGACTATGTACCCCATTGTCGAATCTGTGCTCCAACCGGGATGTGTATGCGTTTTGGGATCCTTTCCATCCATCTGAGCGGGCGAACCGGCTGATTGTACAGACGATCATGACCGGGTCTAGCAAGTACATGAGCCCTATGAACCTAAGCACCATCATGGCCATGGACTCCGGGAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

406

Amino Acids

44.08

Weight (kDa)

9.15

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 72 - 380 3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 602
Acc65I GGTACC 1 cut(s) 652
AccB1I GGYRCC 2 cut(s) 125, 652
AccBSI CCGCTC 1 cut(s) 1117
AccII CGCG 2 cut(s) 272, 274
AccIII TCCGGA 1 cut(s) 732
AciI CCGC 9 cut(s) 87, 270, 316, 328, 621, 801, 804, 1014, 1117
AclWI GGATC 4 cut(s) 385, 515, 1091, 1104
AcoI YGGCCR 3 cut(s) 261, 325, 1197
AcsI RAATTY 1 cut(s) 726
AfiI CCNNNNNNNGG 3 cut(s) 293, 720, 1031
AgeI ACCGGT 1 cut(s) 773
AgsI TTSAA 2 cut(s) 488, 871
AluBI AGCT 9 cut(s) 95, 194, 203, 209, 385, 412, 427, 847, 913
AluI AGCT 9 cut(s) 95, 194, 203, 209, 385, 412, 427, 847, 913
Alw21I GWGCWC 5 cut(s) 170, 414, 841, 849, 1070
Alw26I GTCTC 4 cut(s) 76, 108, 219, 730
Alw44I GTGCAC 1 cut(s) 837
AlwI GGATC 4 cut(s) 385, 515, 1091, 1104
Ama87I CYCGRG 1 cut(s) 204
Aor13HI TCCGGA 1 cut(s) 732
AoxI GGCC 5 cut(s) 261, 325, 781, 810, 1197
ApaLI GTGCAC 1 cut(s) 837
ApeKI GCWGC 6 cut(s) 200, 427, 569, 857, 860, 935
ApoI RAATTY 1 cut(s) 726
AsiGI ACCGGT 1 cut(s) 773
Asp718I GGTACC 1 cut(s) 652
AspLEI GCGC 2 cut(s) 274, 574
AspS9I GGNCC 3 cut(s) 782, 811, 1022
AsuC2I CCSGG 4 cut(s) 976, 1076, 1153, 1210
AsuHPI GGTGA 4 cut(s) 184, 406, 781, 842
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 1 cut(s) 1022
BaeGI GKGCMC 2 cut(s) 559, 841
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BalI TGGCCA 2 cut(s) 263, 1199
BamHI GGATCC 1 cut(s) 1096
BanI GGYRCC 2 cut(s) 125, 652
BanII GRGCYC 4 cut(s) 414, 849, 903, 1175
Bbv12I GWGCWC 5 cut(s) 170, 414, 841, 849, 1070
BbvCI CCTCAGC 1 cut(s) 195
BbvI GCAGC 6 cut(s) 212, 414, 581, 869, 872, 922
BccI CCATC 4 cut(s) 883, 1113, 1117, 1199
BceAI ACGGC 3 cut(s) 259, 310, 355
BcgI CGANNNNNNTGC 2 cut(s) 613, 647
BcnI CCSGG 4 cut(s) 976, 1076, 1153, 1210
BcoDI GTCTC 4 cut(s) 76, 108, 219, 730
BfaI CTAG 6 cut(s) 39, 111, 146, 386, 1158, 1219
BfmI CTRYAG 1 cut(s) 66
BglI GCCNNNNNGGC 4 cut(s) 134, 324, 789, 809
BisI GCNGC 9 cut(s) 201, 328, 428, 570, 622, 804, 858, 861, 936
BlpI GCTNAGC 1 cut(s) 909
BlsI GCNGC 9 cut(s) 202, 329, 429, 571, 623, 805, 859, 862, 937
BmcAI AGTACT 2 cut(s) 680, 695
Bme1390I CCNGG 4 cut(s) 976, 1076, 1153, 1210
Bme18I GGWCC 1 cut(s) 1022
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 3 cut(s) 782, 811, 1022
BmiI GGNNCC 3 cut(s) 127, 654, 1098
BmrFI CCNGG 4 cut(s) 976, 1076, 1153, 1210
BmrI ACTGGG 1 cut(s) 694
BmuI ACTGGG 1 cut(s) 694
BpmI CTGGAG 1 cut(s) 833
Bpu10I CCTNAGC 2 cut(s) 195, 1184
Bpu1102I GCTNAGC 1 cut(s) 909
BpuMI CCSGG 4 cut(s) 976, 1076, 1153, 1210
BsaJI CCNNGG 3 cut(s) 132, 974, 1200
BsaWI WCCGGW 5 cut(s) 122, 416, 720, 732, 773
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 831, 861
Bsc4I CCNNNNNNNGG 3 cut(s) 293, 720, 1031
Bse118I RCCGGY 3 cut(s) 323, 773, 1125
Bse1I ACTGG 3 cut(s) 484, 676, 700
Bse3DI GCAATG 3 cut(s) 168, 631, 930
BseAI TCCGGA 1 cut(s) 732
BseDI CCNNGG 3 cut(s) 132, 974, 1200
BseGI GGATG 2 cut(s) 1084, 1105
BseLI CCNNNNNNNGG 3 cut(s) 293, 720, 1031
BseMI GCAATG 3 cut(s) 168, 631, 930
BseMII CTCAG 5 cut(s) 186, 704, 923, 979, 1104
BseNI ACTGG 3 cut(s) 484, 676, 700
BseRI GAGGAG 2 cut(s) 96, 832
BseSI GKGCMC 2 cut(s) 559, 841
BseX3I CGGCCG 1 cut(s) 325
BseXI GCAGC 6 cut(s) 212, 414, 581, 869, 872, 922
Bsh1236I CGCG 2 cut(s) 272, 274
Bsh1285I CGRYCG 1 cut(s) 328
BshFI GGCC 5 cut(s) 263, 327, 783, 812, 1199
BshNI GGYRCC 2 cut(s) 125, 652
BshTI ACCGGT 1 cut(s) 773
BsiEI CGRYCG 1 cut(s) 328
BsiHKAI GWGCWC 5 cut(s) 170, 414, 841, 849, 1070
BsiHKCI CYCGRG 1 cut(s) 204
BslFI GGGAC 1 cut(s) 1053
BslI CCNNNNNNNGG 3 cut(s) 293, 720, 1031
BsmAI GTCTC 4 cut(s) 76, 108, 219, 730
BsmFI GGGAC 1 cut(s) 1053
BsnI GGCC 5 cut(s) 263, 327, 783, 812, 1199
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 8 cut(s) 170, 414, 559, 841, 849, 903, 1070, 1175
Bsp13I TCCGGA 1 cut(s) 732
Bsp1407I TGTACA 1 cut(s) 1135
Bsp143I GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp19I CCATGG 2 cut(s) 132, 1200
BspACI CCGC 9 cut(s) 87, 270, 316, 328, 621, 801, 804, 1014, 1117
BspANI GGCC 5 cut(s) 263, 327, 783, 812, 1199
BspCNI CTCAG 5 cut(s) 187, 703, 922, 978, 1105
BspEI TCCGGA 1 cut(s) 732
BspFNI CGCG 2 cut(s) 272, 274
BspHI TCATGA 1 cut(s) 1146
BspLI GGNNCC 3 cut(s) 127, 654, 1098
BspPI GGATC 4 cut(s) 385, 515, 1091, 1104
BspT107I GGYRCC 2 cut(s) 125, 652
BsrBI CCGCTC 1 cut(s) 1117
BsrDI GCAATG 3 cut(s) 168, 631, 930
BsrFI RCCGGY 3 cut(s) 323, 773, 1125
BsrGI TGTACA 1 cut(s) 1135
BsrI ACTGG 3 cut(s) 484, 676, 700
BssAI RCCGGY 3 cut(s) 323, 773, 1125
BssECI CCNNGG 3 cut(s) 132, 974, 1200
BssMI GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
BssT1I CCWWGG 2 cut(s) 132, 1200
Bst4CI ACNGT 3 cut(s) 67, 172, 1026
BstAUI TGTACA 1 cut(s) 1135
BstC8I GCNNGC 5 cut(s) 276, 368, 576, 1016, 1119
BstDEI CTNAG 6 cut(s) 195, 690, 909, 965, 1113, 1184
BstDSI CCRYGG 2 cut(s) 132, 1200
BstEII GGTNACC 1 cut(s) 769
BstF5I GGATG 2 cut(s) 1084, 1105
BstFNI CGCG 2 cut(s) 272, 274
BstHHI GCGC 2 cut(s) 274, 574
BstKTI GATC 6 cut(s) 358, 380, 510, 671, 1099, 1146
BstMAI GTCTC 4 cut(s) 76, 108, 219, 730
BstMBI GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
BstMCI CGRYCG 1 cut(s) 328
BstNSI RCATGY 1 cut(s) 1011
BstPI GGTNACC 1 cut(s) 769
BstSCI CCNGG 4 cut(s) 974, 1074, 1151, 1208
BstSFI CTRYAG 1 cut(s) 66
BstSLI GKGCMC 2 cut(s) 559, 841
BstUI CGCG 2 cut(s) 272, 274
BstV1I GCAGC 6 cut(s) 212, 414, 581, 869, 872, 922
BstX2I RGATCY 2 cut(s) 507, 1096
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 2 cut(s) 507, 1096
BstZI CGGCCG 1 cut(s) 325
BsuRI GGCC 5 cut(s) 263, 327, 783, 812, 1199
BtgI CCRYGG 2 cut(s) 132, 1200
BtsCI GGATG 2 cut(s) 1084, 1105
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 5 cut(s) 276, 368, 576, 1016, 1119
CciI TCATGA 1 cut(s) 1146
CfoI GCGC 2 cut(s) 274, 574
Cfr10I RCCGGY 3 cut(s) 323, 773, 1125
Cfr13I GGNCC 3 cut(s) 782, 811, 1022
CseI GACGC 2 cut(s) 605, 932
CspAI ACCGGT 1 cut(s) 773
CviAII CATG 7 cut(s) 100, 133, 1008, 1147, 1168, 1195, 1201
DdeI CTNAG 6 cut(s) 195, 690, 909, 965, 1113, 1184
DpnI GATC 6 cut(s) 357, 379, 509, 670, 1098, 1145
DpnII GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 3 cut(s) 261, 325, 1197
EagI CGGCCG 1 cut(s) 325
Ecl136II GAGCTC 2 cut(s) 412, 847
EclXI CGGCCG 1 cut(s) 325
Eco130I CCWWGG 2 cut(s) 132, 1200
Eco24I GRGCYC 4 cut(s) 414, 849, 903, 1175
Eco47I GGWCC 1 cut(s) 1022
Eco52I CGGCCG 1 cut(s) 325
Eco53kI GAGCTC 2 cut(s) 412, 847
Eco88I CYCGRG 1 cut(s) 204
Eco91I GGTNACC 1 cut(s) 769
EcoICRI GAGCTC 2 cut(s) 412, 847
EcoO65I GGTNACC 1 cut(s) 769
EcoT14I CCWWGG 2 cut(s) 132, 1200
EcoT38I GRGCYC 4 cut(s) 414, 849, 903, 1175
ErhI CCWWGG 2 cut(s) 132, 1200
FaeI CATG 7 cut(s) 103, 136, 1011, 1150, 1171, 1198, 1204
FaqI GGGAC 1 cut(s) 1053
FatI CATG 7 cut(s) 99, 132, 1007, 1146, 1167, 1194, 1200
FauI CCCGC 4 cut(s) 94, 808, 1007, 1110
Fnu4HI GCNGC 9 cut(s) 201, 328, 428, 570, 622, 804, 858, 861, 936
FokI GGATG 2 cut(s) 1091, 1092
FriOI GRGCYC 4 cut(s) 414, 849, 903, 1175
Fsp4HI GCNGC 9 cut(s) 201, 328, 428, 570, 622, 804, 858, 861, 936
FspBI CTAG 6 cut(s) 39, 111, 146, 386, 1158, 1219
GlaI GCGC 2 cut(s) 273, 573
GluI GCNGC 9 cut(s) 201, 328, 428, 570, 622, 804, 858, 861, 936
GsuI CTGGAG 1 cut(s) 833
HaeIII GGCC 5 cut(s) 263, 327, 783, 812, 1199
HgaI GACGC 2 cut(s) 605, 932
HhaI GCGC 2 cut(s) 274, 574
Hin1II CATG 7 cut(s) 103, 136, 1011, 1150, 1171, 1198, 1204
Hin6I GCGC 2 cut(s) 272, 572
HinP1I GCGC 2 cut(s) 272, 572
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HinfI GANTC 5 cut(s) 175, 227, 465, 1060, 1205
HphI GGTGA 4 cut(s) 184, 406, 781, 842
Hpy166II GTNNAC 3 cut(s) 592, 637, 839
Hpy188I TCNGA 5 cut(s) 224, 382, 566, 715, 1114
Hpy188III TCNNGA 4 cut(s) 733, 850, 886, 1147
Hpy8I GTNNAC 3 cut(s) 592, 637, 839
Hpy99I CGWCG 1 cut(s) 599
HpyAV CCTTC 2 cut(s) 667, 753
HpyCH4III ACNGT 3 cut(s) 67, 172, 1026
HpyCH4IV ACGT 1 cut(s) 995
HpyCH4V TGCA 3 cut(s) 665, 839, 950
HpyF3I CTNAG 6 cut(s) 195, 690, 909, 965, 1113, 1184
HpySE526I ACGT 1 cut(s) 995
Hsp92II CATG 7 cut(s) 103, 136, 1011, 1150, 1171, 1198, 1204
HspAI GCGC 2 cut(s) 272, 572
Kpn2I TCCGGA 1 cut(s) 732
KpnI GGTACC 1 cut(s) 656
Kzo9I GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
LmnI GCTCC 4 cut(s) 382, 566, 852, 1073
Lsp1109I GCAGC 6 cut(s) 212, 414, 581, 869, 872, 922
MaeI CTAG 6 cut(s) 39, 111, 146, 386, 1158, 1219
MaeII ACGT 1 cut(s) 995
MaeIII GTNAC 2 cut(s) 769, 968
MalI GATC 6 cut(s) 357, 379, 509, 670, 1098, 1145
MbiI CCGCTC 1 cut(s) 1117
MboI GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
MflI RGATCY 2 cut(s) 507, 1096
MhlI GDGCHC 8 cut(s) 170, 414, 559, 841, 849, 903, 1070, 1175
MlsI TGGCCA 2 cut(s) 263, 1199
MluCI AATT 5 cut(s) 57, 253, 459, 488, 726
MluNI TGGCCA 2 cut(s) 263, 1199
MlyI GAGTC 2 cut(s) 221, 1199
MmeI TCCRAC 2 cut(s) 360, 1095
Mox20I TGGCCA 2 cut(s) 263, 1199
MroI TCCGGA 1 cut(s) 732
MscI TGGCCA 2 cut(s) 263, 1199
MseI TTAA 2 cut(s) 56, 344
MslI CAYNNNNRTG 2 cut(s) 1193, 1199
Msp20I TGGCCA 2 cut(s) 263, 1199
MspR9I CCNGG 4 cut(s) 976, 1076, 1153, 1210
MvnI CGCG 2 cut(s) 272, 274
NciI CCSGG 4 cut(s) 976, 1076, 1153, 1210
NcoI CCATGG 2 cut(s) 132, 1200
NdeII GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
NlaIII CATG 7 cut(s) 103, 136, 1011, 1150, 1171, 1198, 1204
NlaIV GGNNCC 3 cut(s) 127, 654, 1098
NmeAIII GCCGAG 1 cut(s) 597
NmuCI GTSAC 1 cut(s) 769
NspI RCATGY 1 cut(s) 1011
PaeR7I CTCGAG 1 cut(s) 204
PagI TCATGA 1 cut(s) 1146
PfeI GAWTC 3 cut(s) 175, 465, 1060
PflFI GACNNNGTC 2 cut(s) 632, 1153
PfoI TCCNGGA 1 cut(s) 1208
PinAI ACCGGT 1 cut(s) 773
PkrI GCNGC 9 cut(s) 202, 329, 429, 571, 623, 805, 859, 862, 937
PleI GAGTC 2 cut(s) 221, 1199
PpsI GAGTC 2 cut(s) 221, 1199
Psp124BI GAGCTC 2 cut(s) 414, 849
PspEI GGTNACC 1 cut(s) 769
PspN4I GGNNCC 3 cut(s) 127, 654, 1098
PspPI GGNCC 3 cut(s) 782, 811, 1022
PspXI VCTCGAGB 1 cut(s) 204
PsuI RGATCY 2 cut(s) 507, 1096
PsyI GACNNNGTC 2 cut(s) 632, 1153
RseI CAYNNNNRTG 2 cut(s) 1193, 1199
SacI GAGCTC 2 cut(s) 414, 849
SaqAI TTAA 2 cut(s) 56, 344
SatI GCNGC 9 cut(s) 201, 328, 428, 570, 622, 804, 858, 861, 936
Sau3AI GATC 6 cut(s) 355, 377, 507, 668, 1096, 1143
Sau96I GGNCC 3 cut(s) 782, 811, 1022
ScaI AGTACT 2 cut(s) 680, 695
SchI GAGTC 2 cut(s) 221, 1199
ScrFI CCNGG 4 cut(s) 976, 1076, 1153, 1210
SduI GDGCHC 8 cut(s) 170, 414, 559, 841, 849, 903, 1070, 1175
SfcI CTRYAG 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 1022
SlaI CTCGAG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 1193, 1199
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 5 cut(s) 57, 253, 459, 488, 726
SsiI CCGC 9 cut(s) 87, 270, 316, 328, 621, 801, 804, 1014, 1117
SspMI CTAG 6 cut(s) 39, 111, 146, 386, 1158, 1219
SstI GAGCTC 2 cut(s) 414, 849
StyD4I CCNGG 4 cut(s) 974, 1074, 1151, 1208
StyI CCWWGG 2 cut(s) 132, 1200
TaaI ACNGT 3 cut(s) 67, 172, 1026
TaiI ACGT 1 cut(s) 998
TaqI TCGA 2 cut(s) 205, 1058
TasI AATT 5 cut(s) 57, 253, 459, 488, 726
TatI WGTACW 4 cut(s) 678, 693, 1135, 1164
TauI GCSGC 3 cut(s) 330, 624, 806
TfiI GAWTC 3 cut(s) 175, 465, 1060
Tru1I TTAA 2 cut(s) 56, 344
Tru9I TTAA 2 cut(s) 56, 344
TscAI CASTG 1 cut(s) 412
TseFI GTSAC 1 cut(s) 769
TseI GCWGC 6 cut(s) 200, 427, 569, 857, 860, 935
Tsp45I GTSAC 1 cut(s) 769
TspDTI ATGAA 3 cut(s) 88, 919, 1193
TspRI CASTG 1 cut(s) 412
Tth111I GACNNNGTC 2 cut(s) 632, 1153
VneI GTGCAC 1 cut(s) 837
VpaK11BI GGWCC 1 cut(s) 1022
XapI RAATTY 1 cut(s) 726
XceI RCATGY 1 cut(s) 1011
XcmI CCANNNNNNNNNTGG 1 cut(s) 1198
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 6 cut(s) 39, 111, 146, 386, 1158, 1219
ZrmI AGTACT 2 cut(s) 680, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.