Rmu_co8252409.1_g000001

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8252409.1
OG0012747
Physical Location & Seq
Forward (+)
173 .. 771
599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8252409.1_g000001.1.cds

Sequence Viewer

Length: 492 bp
atgcagagactatttgaattaggagggagaagggtgctggtgaccggtacaggcccattaggctgtgttcccgccgctatggcccagaggagtaggaatggtgaatgtgcaccagagctccagagggcagcagccatcttcaatcccctgcttgtccagatggttcaggggctcaacgctcagcttggtactgacgctttcattgctgctaatgcgtttgcaaatgtggagttcctcagtaacccccggaaatatggctttgttacgtcaaaggtggcatgttgcgggcaaggaccgtacaatgggttgggactatgtaccccattgtcgaatctgtgctccaaccgggatgtgtatgcgttttgggatcctttccatccatctgagcgggcgaaccggctgattgtgcagacgatcatggccgggtctagcaagtacatgagccctatgaacctaagcaccatcatggccatggactccgggaataactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

163

Amino Acids

17.54

Weight (kDa)

8.86

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 388
AciI CCGC 4 cut(s) 72, 75, 285, 388
AclWI GGATC 2 cut(s) 362, 375
AcoI YGGCCR 2 cut(s) 420, 468
AfaI GTAC 5 cut(s) 49, 190, 299, 319, 437
AfiI CCNNNNNNNGG 1 cut(s) 302
AgeI ACCGGT 1 cut(s) 44
AgsI TTSAA 2 cut(s) 17, 142
AluBI AGCT 2 cut(s) 118, 184
AluI AGCT 2 cut(s) 118, 184
Alw21I GWGCWC 3 cut(s) 112, 120, 341
Alw44I GTGCAC 1 cut(s) 108
AlwI GGATC 2 cut(s) 362, 375
AoxI GGCC 4 cut(s) 52, 81, 420, 468
ApaLI GTGCAC 1 cut(s) 108
ApeKI GCWGC 3 cut(s) 128, 131, 206
AsiGI ACCGGT 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 53, 82, 293
AsuC2I CCSGG 4 cut(s) 247, 347, 424, 481
AsuHPI GGTGA 2 cut(s) 52, 113
AvaII GGWCC 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 112
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 1 cut(s) 367
BanII GRGCYC 3 cut(s) 120, 174, 446
Bbv12I GWGCWC 3 cut(s) 112, 120, 341
BbvI GCAGC 3 cut(s) 140, 143, 193
BccI CCATC 5 cut(s) 143, 154, 384, 388, 470
BcnI CCSGG 4 cut(s) 247, 347, 424, 481
BfaI CTAG 2 cut(s) 429, 490
BglI GCCNNNNNGGC 2 cut(s) 60, 80
BisI GCNGC 4 cut(s) 75, 129, 132, 207
BlpI GCTNAGC 1 cut(s) 180
BlsI GCNGC 4 cut(s) 76, 130, 133, 208
Bme1390I CCNGG 4 cut(s) 247, 347, 424, 481
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 3 cut(s) 53, 82, 293
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 4 cut(s) 247, 347, 424, 481
BpmI CTGGAG 1 cut(s) 104
Bpu10I CCTNAGC 1 cut(s) 455
Bpu1102I GCTNAGC 1 cut(s) 180
BpuMI CCSGG 4 cut(s) 247, 347, 424, 481
BsaJI CCNNGG 2 cut(s) 245, 471
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse118I RCCGGY 2 cut(s) 44, 396
Bse3DI GCAATG 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 245, 471
BseGI GGATG 2 cut(s) 355, 376
BseLI CCNNNNNNNGG 1 cut(s) 302
BseMI GCAATG 1 cut(s) 201
BseMII CTCAG 3 cut(s) 194, 250, 375
BseRI GAGGAG 1 cut(s) 103
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 3 cut(s) 140, 143, 193
BsgI GTGCAG 1 cut(s) 428
BshFI GGCC 4 cut(s) 54, 83, 422, 470
BshTI ACCGGT 1 cut(s) 44
BsiHKAI GWGCWC 3 cut(s) 112, 120, 341
BsiSI CCGG 6 cut(s) 45, 247, 346, 397, 423, 480
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 324
BsnI GGCC 4 cut(s) 54, 83, 422, 470
Bsp1286I GDGCHC 5 cut(s) 112, 120, 174, 341, 446
Bsp143I GATC 2 cut(s) 367, 414
Bsp1720I GCTNAGC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 4 cut(s) 72, 75, 285, 388
BspANI GGCC 4 cut(s) 54, 83, 422, 470
BspCNI CTCAG 3 cut(s) 193, 249, 376
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 2 cut(s) 362, 375
BsrBI CCGCTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 201
BsrFI RCCGGY 2 cut(s) 44, 396
BssAI RCCGGY 2 cut(s) 44, 396
BssECI CCNNGG 2 cut(s) 245, 471
BssMI GATC 2 cut(s) 367, 414
BssT1I CCWWGG 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 287, 390
BstDEI CTNAG 4 cut(s) 180, 236, 384, 455
BstDSI CCRYGG 1 cut(s) 471
BstEII GGTNACC 1 cut(s) 40
BstF5I GGATG 2 cut(s) 355, 376
BstKTI GATC 2 cut(s) 370, 417
BstMBI GATC 2 cut(s) 367, 414
BstMWI GCNNNNNNNGC 5 cut(s) 60, 80, 203, 212, 406
BstNSI RCATGY 1 cut(s) 282
BstPI GGTNACC 1 cut(s) 40
BstSCI CCNGG 4 cut(s) 245, 345, 422, 479
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 140, 143, 193
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
BsuRI GGCC 4 cut(s) 54, 83, 422, 470
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 2 cut(s) 355, 376
Cac8I GCNNGC 2 cut(s) 287, 390
Cfr10I RCCGGY 2 cut(s) 44, 396
Cfr13I GGNCC 3 cut(s) 53, 82, 293
CseI GACGC 1 cut(s) 203
Csp6I GTAC 5 cut(s) 48, 189, 298, 318, 436
CspAI ACCGGT 1 cut(s) 44
CviAII CATG 5 cut(s) 279, 418, 439, 466, 472
CviQI GTAC 5 cut(s) 48, 189, 298, 318, 436
DdeI CTNAG 4 cut(s) 180, 236, 384, 455
DpnI GATC 2 cut(s) 369, 416
DpnII GATC 2 cut(s) 367, 414
EaeI YGGCCR 2 cut(s) 420, 468
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 471
Eco24I GRGCYC 3 cut(s) 120, 174, 446
Eco47I GGWCC 1 cut(s) 293
Eco53kI GAGCTC 1 cut(s) 118
Eco91I GGTNACC 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 471
EcoT38I GRGCYC 3 cut(s) 120, 174, 446
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 5 cut(s) 282, 421, 442, 469, 475
FaqI GGGAC 1 cut(s) 324
FatI CATG 5 cut(s) 278, 417, 438, 465, 471
FauI CCCGC 3 cut(s) 79, 278, 381
Fnu4HI GCNGC 4 cut(s) 75, 129, 132, 207
FokI GGATG 2 cut(s) 362, 363
FriOI GRGCYC 3 cut(s) 120, 174, 446
Fsp4HI GCNGC 4 cut(s) 75, 129, 132, 207
FspBI CTAG 2 cut(s) 429, 490
GluI GCNGC 4 cut(s) 75, 129, 132, 207
GsuI CTGGAG 1 cut(s) 104
HaeIII GGCC 4 cut(s) 54, 83, 422, 470
HapII CCGG 6 cut(s) 45, 247, 346, 397, 423, 480
HgaI GACGC 1 cut(s) 203
Hin1II CATG 5 cut(s) 282, 421, 442, 469, 475
HinfI GANTC 2 cut(s) 331, 476
HpaII CCGG 6 cut(s) 45, 247, 346, 397, 423, 480
HphI GGTGA 2 cut(s) 52, 113
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 1 cut(s) 385
Hpy188III TCNNGA 2 cut(s) 121, 157
Hpy8I GTNNAC 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 24
HpyCH4III ACNGT 1 cut(s) 297
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 4 cut(s) 4, 110, 221, 409
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 80, 203, 212, 406
HpyF3I CTNAG 4 cut(s) 180, 236, 384, 455
HpySE526I ACGT 1 cut(s) 266
Hsp92II CATG 5 cut(s) 282, 421, 442, 469, 475
Kzo9I GATC 2 cut(s) 367, 414
LmnI GCTCC 2 cut(s) 123, 344
Lsp1109I GCAGC 3 cut(s) 140, 143, 193
MaeI CTAG 2 cut(s) 429, 490
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 40, 239, 262
MalI GATC 2 cut(s) 369, 416
MbiI CCGCTC 1 cut(s) 388
MboI GATC 2 cut(s) 367, 414
MboII GAAGA 1 cut(s) 130
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 5 cut(s) 112, 120, 174, 341, 446
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 1 cut(s) 17
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 1 cut(s) 470
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 4 cut(s) 17, 81, 117, 245
Mox20I TGGCCA 1 cut(s) 470
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 2 cut(s) 464, 470
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 6 cut(s) 45, 247, 346, 397, 423, 480
MspR9I CCNGG 4 cut(s) 247, 347, 424, 481
MwoI GCNNNNNNNGC 5 cut(s) 60, 80, 203, 212, 406
NciI CCSGG 4 cut(s) 247, 347, 424, 481
NcoI CCATGG 1 cut(s) 471
NdeII GATC 2 cut(s) 367, 414
NlaIII CATG 5 cut(s) 282, 421, 442, 469, 475
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 1 cut(s) 40
NspI RCATGY 1 cut(s) 282
PfeI GAWTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 479
PinAI ACCGGT 1 cut(s) 44
PkrI GCNGC 4 cut(s) 76, 130, 133, 208
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 120
PspEI GGTNACC 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 3 cut(s) 53, 82, 293
PsuI RGATCY 1 cut(s) 367
RsaI GTAC 5 cut(s) 49, 190, 299, 319, 437
RsaNI GTAC 5 cut(s) 48, 189, 298, 318, 436
RseI CAYNNNNRTG 2 cut(s) 464, 470
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 4 cut(s) 75, 129, 132, 207
Sau3AI GATC 2 cut(s) 367, 414
Sau96I GGNCC 3 cut(s) 53, 82, 293
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 4 cut(s) 247, 347, 424, 481
SduI GDGCHC 5 cut(s) 112, 120, 174, 341, 446
SetI ASST 5 cut(s) 120, 186, 269, 276, 456
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 464, 470
Sse9I AATT 1 cut(s) 17
SsiI CCGC 4 cut(s) 72, 75, 285, 388
SspMI CTAG 2 cut(s) 429, 490
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 4 cut(s) 245, 345, 422, 479
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 297
TaiI ACGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 329
TasI AATT 1 cut(s) 17
TatI WGTACW 1 cut(s) 435
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 331
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 3 cut(s) 128, 131, 206
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 2 cut(s) 190, 464
VneI GTGCAC 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 293
XceI RCATGY 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 469
XspI CTAG 2 cut(s) 429, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.