Rorug06G0175600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0012747
Physical Location & Seq
Forward (+)
27609335 .. 27609694
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0175600.1

Sequence Viewer

Length: 360 bp
ATGGACACCGGAGCGGCGGGAGTGGGTCTCGCCGCGTCGTCGTCGTCGGCGGAGGACCCGTCGTCTCGGGGGATCGCCGCTCAACGCCGTCCAGGCGTGGAGCTTCGGCGTGGAGCTTCGGCGTGTCGCGCCGATACCAGCACCTGCTCGACAAAACGACGCCGCACGTCCTCCACCGCTGGCTCGCGGCGTGGCCTTGGTCTACGTCCTCCGCGTCTACTTCGTCCAGGGATTCTACATCATGCCATACGGCTTGGGGATCTACCTCCTAAACTTGCTCATCGGCTTTCTCTCTCCTCAGGTCGACCTGAAGATCCACGACCTTTCCGACGGCCCCAGCCTCCCGACCCGCGGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

119

Amino Acids

12.67

Weight (kDa)

11.88

Isoelectric Point (pI)

78.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28780
fragaria_vesca FvH4_2g19880
malus_domestica MD05G1183000.v1.1 MD10G1171100.v1.1
prunus_persica Prupe.8G209600_v2.0.a1
pyrus_communis pycom10g14750
rosa_chinensis RchiOBHm_Chr6g0285571
rosa_laevigata RLG00000012626
rosa_multiflora Rmu_co8252409.1_g000001 Rmu_sc0017770.1_g000005
rosa_roxburghii Rroxscaffold_7G00182950
rosa_rugosa Rorug06G0175600 Rorug06G0175700.1
rosa_samantha Rh6AG287200 Rh6BG288400 Rh6CG289600 Rh6DG282400
rosa_wichuraiana Rw6G024690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 152
Acc36I ACCTGC 1 cut(s) 152
AccBSI CCGCTC 2 cut(s) 14, 80
AccI GTMKAC 3 cut(s) 202, 217, 304
AccII CGCG 5 cut(s) 35, 129, 187, 214, 352
AclWI GGATC 3 cut(s) 80, 267, 308
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 160
AfiI CCNNNNNNNGG 1 cut(s) 351
AhdI GACNNNNNGTC 1 cut(s) 61
AjiI CACGTC 1 cut(s) 168
AjnI CCWGG 2 cut(s) 91, 226
AluBI AGCT 2 cut(s) 103, 116
AluI AGCT 2 cut(s) 103, 116
Alw26I GTCTC 2 cut(s) 32, 69
AlwI GGATC 3 cut(s) 80, 267, 308
AlwNI CAGNNNCTG 1 cut(s) 144
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 2 cut(s) 193, 332
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 55, 333
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 55
AxyI CCTNAGG 1 cut(s) 298
BceAI ACGGC 3 cut(s) 72, 266, 347
BciT130I CCWGG 2 cut(s) 93, 228
BcoDI GTCTC 2 cut(s) 32, 69
BfuAI ACCTGC 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 5 cut(s) 15, 33, 78, 163, 188
BlsI GCNGC 5 cut(s) 16, 34, 79, 164, 189
Bme1390I CCNGG 2 cut(s) 93, 228
Bme18I GGWCC 1 cut(s) 55
BmeRI GACNNNNNGTC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 168
BmgT120I GGNCC 2 cut(s) 55, 333
BmiI GGNNCC 2 cut(s) 57, 335
BmrFI CCNGG 2 cut(s) 93, 228
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 196, 227, 350
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 1 cut(s) 351
Bse21I CCTNAGG 1 cut(s) 298
BseBI CCWGG 2 cut(s) 93, 228
BseDI CCNNGG 3 cut(s) 196, 227, 350
BseLI CCNNNNNNNGG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 286
BseYI CCCAGC 1 cut(s) 336
Bsh1236I CGCG 5 cut(s) 35, 129, 187, 214, 352
BshFI GGCC 2 cut(s) 195, 334
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 9
BslI CCNNNNNNNGG 1 cut(s) 351
BsmAI GTCTC 2 cut(s) 32, 69
BsmBI CGTCTC 1 cut(s) 69
BsnI GGCC 2 cut(s) 195, 334
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 66
Bsp143I GATC 3 cut(s) 72, 259, 313
BspANI GGCC 2 cut(s) 195, 334
BspCNI CTCAG 1 cut(s) 311
BspFNI CGCG 5 cut(s) 35, 129, 187, 214, 352
BspLI GGNNCC 2 cut(s) 57, 335
BspMI ACCTGC 1 cut(s) 152
BspPI GGATC 3 cut(s) 80, 267, 308
BspTNI GGTCTC 1 cut(s) 32
BsrBI CCGCTC 2 cut(s) 14, 80
BssECI CCNNGG 3 cut(s) 196, 227, 350
BssMI GATC 3 cut(s) 72, 259, 313
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 196
Bst2UI CCWGG 2 cut(s) 93, 228
BstACI GRCGYC 1 cut(s) 160
BstC8I GCNNGC 2 cut(s) 181, 185
BstDEI CTNAG 1 cut(s) 298
BstDSI CCRYGG 1 cut(s) 350
BstFNI CGCG 5 cut(s) 35, 129, 187, 214, 352
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 75, 262, 316
BstMAI GTCTC 2 cut(s) 32, 69
BstMBI GATC 3 cut(s) 72, 259, 313
BstMWI GCNNNNNNNGC 2 cut(s) 93, 128
BstNI CCWGG 2 cut(s) 93, 228
BstSCI CCNGG 2 cut(s) 91, 226
BstUI CGCG 5 cut(s) 35, 129, 187, 214, 352
BstX2I RGATCY 2 cut(s) 259, 313
BstYI RGATCY 2 cut(s) 259, 313
Bsu36I CCTNAGG 1 cut(s) 298
BsuRI GGCC 2 cut(s) 195, 334
BtgI CCRYGG 1 cut(s) 350
BtrI CACGTC 1 cut(s) 168
BveI ACCTGC 1 cut(s) 152
Cac8I GCNNGC 2 cut(s) 181, 185
CaiI CAGNNNCTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 55, 333
Cfr42I CCGCGG 1 cut(s) 353
CseI GACGC 3 cut(s) 24, 168, 203
CviAII CATG 1 cut(s) 242
CviJI RGCY 8 cut(s) 103, 116, 183, 195, 253, 286, 334, 340
CviKI_1 RGCY 8 cut(s) 103, 116, 183, 195, 253, 286, 334, 340
DdeI CTNAG 1 cut(s) 298
DpnI GATC 3 cut(s) 74, 261, 315
DpnII GATC 3 cut(s) 72, 259, 313
DriI GACNNNNNGTC 1 cut(s) 61
Eam1105I GACNNNNNGTC 1 cut(s) 61
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 196
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 330
Eco81I CCTNAGG 1 cut(s) 298
Eco88I CYCGRG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 91, 226
EcoT14I CCWWGG 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 196
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 1 cut(s) 245
FaiI YATR 2 cut(s) 243, 248
FatI CATG 1 cut(s) 241
FauI CCCGC 2 cut(s) 10, 357
FblI GTMKAC 3 cut(s) 202, 217, 304
Fnu4HI GCNGC 5 cut(s) 15, 33, 78, 163, 188
Fsp4HI GCNGC 5 cut(s) 15, 33, 78, 163, 188
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 15, 33, 78, 163, 188
GsaI CCCAGC 1 cut(s) 340
HaeIII GGCC 2 cut(s) 195, 334
HapII CCGG 1 cut(s) 9
HgaI GACGC 3 cut(s) 24, 168, 203
HhaI GCGC 1 cut(s) 131
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 1 cut(s) 245
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 305
HindII GTYRAC 1 cut(s) 305
HinfI GANTC 1 cut(s) 232
HpaII CCGG 1 cut(s) 9
Hpy166II GTNNAC 3 cut(s) 203, 218, 305
Hpy188I TCNGA 1 cut(s) 329
Hpy188III TCNNGA 1 cut(s) 344
Hpy8I GTNNAC 3 cut(s) 203, 218, 305
Hpy99I CGWCG 7 cut(s) 40, 43, 46, 49, 64, 162, 333
HpyCH4IV ACGT 2 cut(s) 167, 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 128
HpyF3I CTNAG 1 cut(s) 298
HpySE526I ACGT 2 cut(s) 167, 205
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 1 cut(s) 245
HspAI GCGC 1 cut(s) 129
KspI CCGCGG 1 cut(s) 353
Kzo9I GATC 3 cut(s) 72, 259, 313
LmnI GCTCC 3 cut(s) 11, 100, 113
MaeII ACGT 2 cut(s) 167, 205
MalI GATC 3 cut(s) 74, 261, 315
MbiI CCGCTC 2 cut(s) 14, 80
MboI GATC 3 cut(s) 72, 259, 313
MboII GAAGA 1 cut(s) 323
MflI RGATCY 2 cut(s) 259, 313
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 6 cut(s) 46, 181, 219, 276, 307, 351
MspA1I CMGCKG 2 cut(s) 179, 352
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 93, 228
MvaI CCWGG 2 cut(s) 93, 228
MvnI CGCG 5 cut(s) 35, 129, 187, 214, 352
MwoI GCNNNNNNNGC 2 cut(s) 93, 128
NdeII GATC 3 cut(s) 72, 259, 313
NlaIII CATG 1 cut(s) 245
NlaIV GGNNCC 2 cut(s) 57, 335
PaqCI CACCTGC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 2 cut(s) 211, 325
PfeI GAWTC 1 cut(s) 232
PkrI GCNGC 5 cut(s) 16, 34, 79, 164, 189
PpuMI RGGWCCY 1 cut(s) 55
Psp5II RGGWCCY 1 cut(s) 55
Psp6I CCWGG 2 cut(s) 91, 226
PspFI CCCAGC 1 cut(s) 336
PspGI CCWGG 2 cut(s) 91, 226
PspN4I GGNNCC 2 cut(s) 57, 335
PspPI GGNCC 2 cut(s) 55, 333
PspPPI RGGWCCY 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 259, 313
SacII CCGCGG 1 cut(s) 353
SalI GTCGAC 1 cut(s) 303
SatI GCNGC 5 cut(s) 15, 33, 78, 163, 188
Sau3AI GATC 3 cut(s) 72, 259, 313
Sau96I GGNCC 2 cut(s) 55, 333
ScrFI CCNGG 2 cut(s) 93, 228
SetI ASST 9 cut(s) 105, 118, 146, 170, 208, 268, 304, 310, 325
Sfr303I CCGCGG 1 cut(s) 353
SgrBI CCGCGG 1 cut(s) 353
SinI GGWCC 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 91, 226
StyI CCWWGG 1 cut(s) 196
TaiI ACGT 2 cut(s) 170, 208
TaqI TCGA 2 cut(s) 149, 304
TauI GCSGC 5 cut(s) 17, 35, 80, 165, 190
TfiI GAWTC 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 55
XmiI GTMKAC 3 cut(s) 202, 217, 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.